Umbilical Cord Blood and UC-MSCs Combined with Low-Dose Immunosuppressant in the Treatment of Elderly Patients with Pure Red Cell Aplastic: A Case Series
CONCLUSION: This report provides an effective regimen of using UCB and UC-MSCs combined with low-dose CsA (1-3 mg/kg/d) to treat PRCA, especially for elderly patients with multiple complications who cannot use the conventional dosage.PMID:38698745 | DOI:10.2174/011574888X290378240424075002 (Source: Current Stem Cell Research and Therapy)
Source: Current Stem Cell Research and Therapy - May 3, 2024 Category: Stem Cells Authors: Wei-Wei Zhu Sujing Zhuang Zhe Yu Xin Li Tian-Jie Han Yue Ma Li-Jun Li Zhi-Rui Zhao Source Type: research
Forward programming human pluripotent stem cells into microglia
Trends Cell Biol. 2024 May 3:S0962-8924(24)00069-2. doi: 10.1016/j.tcb.2024.03.006. Online ahead of print.ABSTRACTMicroglia play vital roles in embryonic and post-natal development, homeostasis, and pathogen defence in the central nervous system. Human induced pluripotent stem cell (hiPSC)-based methods have emerged as an important source for the study of human microglia in vitro. Classical approaches to differentiate hiPSCs into microglia suffer from limitations including extended culture periods, consistency, and efficiency. More recently, forward programming has arisen as a promising alternative for the manufacture of b...
Source: Trends in Cell Biology - May 3, 2024 Category: Cytology Authors: J úlia Csatári Heinz Wiendl Matthias Pawlowski Source Type: research
Hyaluronan in mesenchymal stromal cell lineage differentiation from human pluripotent stem cells: application in serum free culture
CONCLUSION: Cultivation of human pluripotent stem cells on a planar substrate of HA in serum-free culture media systems is sufficient to yield a distinctive developmental mesenchymal stromal cell lineage with potential to modify the function of haematopoietic lineages in therapeutic applications.PMID:38702837 | DOI:10.1186/s13287-024-03719-y (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Paul A De Sousa Leo Perfect Jinpei Ye Kay Samuels Ewa Piotrowska Martin Gordon Ryan Mate Elsa Abranches Thomas M Wishart David H Dockrell Aidan Courtney Source Type: research
A GMP-compliant manufacturing method for Wharton's jelly-derived mesenchymal stromal cells
CONCLUSION: This study offers valuable insights into optimizing the isolation and culture of WJ-MSCs. Our scalable manufacturing processes facilitate the large-scale production of high-quality WJ-MSCs. These findings contribute to the advancement of WJ-MSCs-based therapies in regenerative medicine.PMID:38702793 | DOI:10.1186/s13287-024-03725-0 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Wanglong Chu Fen Zhang Xiuping Zeng Fangtao He Guanyan Shang Tao Guo Qingfang Wang Jianfu Wu Tongjing Li Zhen Zhong Zhong Xiao Liang Junyuan Hu Muyun Liu Source Type: research
Monocytes prevent apoptosis of iPSCs and promote differentiation of kidney organoids
CONCLUSION: Our data suggest that co-culturing of iPSCs with human monocytes strongly improves differentiation of kidney organoids. An underlying mechanism of monocytic action implies, but not limited to, an increased autophagy in CHIR-treated iPSCs. Our findings enhance the utility of kidney organoid models.PMID:38702808 | DOI:10.1186/s13287-024-03739-8 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Ekaterina Pecksen Sergey Tkachuk Cristoph Schr öder Marc Vives Enrich Anindita Neog Cory P Johnson Niko Lachmann Hermann Haller Yulia Kiyan Source Type: research
Metabolic reprogramming of tumor-associated macrophages using glutamine antagonist JHU083 drives tumor immunity in myeloid-rich prostate and bladder cancer tumors
Cancer Immunol Res. 2024 May 3. doi: 10.1158/2326-6066.CIR-23-1105. Online ahead of print.ABSTRACTGlutamine metabolism in tumor microenvironments critically regulates anti-tumor immunity. Using glutamine-antagonist prodrug JHU083, we report potent tumor growth inhibition in urologic tumors by JHU083-reprogrammed tumor-associated macrophages (TAMs) and tumor-infiltrating monocytes (TIMs). We show JHU083-mediated glutamine antagonism in tumor microenvironments induces TNF, pro-inflammatory, and mTORC1 signaling in intratumoral TAM clusters. JHU083-reprogrammed TAMs also exhibit increased tumor cell phagocytosis and diminishe...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Monali Praharaj Fan Shen Alex J Lee Liang Zhao Thomas R Nirschl Debebe Theodros Alok K Singh Xiaoxu Wang Kenneth M Adusei Kara A Lombardo Raekwon A Williams Laura A Sena Elizabeth A Thompson Ada Tam Srinivasan Yegnasubramanian Edward J Pearce Robert D Leo Source Type: research
Vaspin facilitates the proliferation and osteogenic differentiation of periodontal ligament stem cells
CONCLUSIONS: In summary, vaspin observably reduces HG-mediated inhibition of PDLSCs OD by modulating the TGF-β1/Smad signaling pathway. Vaspin may be a potential therapeutic for periodontal tissue regeneration in diabetic patients.PMID:38699861 | DOI:10.1111/jre.13254 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Zhihui Jing Xinran Feng Xin Li Xiaoyu Zhang Chunling Pan Source Type: research
Nirmatrelvir/ritonavir treatment of patients with COVID-19 taking tacrolimus: case series describing the results of drug-drug interactions
We describe the cases of two patients treated with nirmatrelvir/ritonavir: a patient who had undergone kidney transplantation and another with a history of hematopoietic stem cell transplantation. Toxic concentrations of tacrolimus were induced in both. This case series highlights the risk associated with the concomitant administration of tacrolimus and nirmatrelvir/ritonavir.PMID:38698526 | DOI:10.1177/03000605241247705 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Dan Shen Yinhua Gong Yulan Qian Jianguo Zhu Jie Gao Source Type: research
Umbilical Cord Blood and UC-MSCs Combined with Low-Dose Immunosuppressant in the Treatment of Elderly Patients with Pure Red Cell Aplastic: A Case Series
CONCLUSION: This report provides an effective regimen of using UCB and UC-MSCs combined with low-dose CsA (1-3 mg/kg/d) to treat PRCA, especially for elderly patients with multiple complications who cannot use the conventional dosage.PMID:38698745 | DOI:10.2174/011574888X290378240424075002 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Wei-Wei Zhu Sujing Zhuang Zhe Yu Xin Li Tian-Jie Han Yue Ma Li-Jun Li Zhi-Rui Zhao Source Type: research
AI identifies potent inducers of breast cancer stem cell differentiation based on adversarial learning from gene expression data
Brief Bioinform. 2024 Mar 27;25(3):bbae207. doi: 10.1093/bib/bbae207.ABSTRACTCancer stem cells (CSCs) are a subpopulation of cancer cells within tumors that exhibit stem-like properties and represent a potentially effective therapeutic target toward long-term remission by means of differentiation induction. By leveraging an artificial intelligence approach solely based on transcriptomics data, this study scored a large library of small molecules based on their predicted ability to induce differentiation in stem-like cells. In particular, a deep neural network model was trained using publicly available single-cell RNA-Seq d...
Source: Briefings in Bioinformatics - May 3, 2024 Category: Bioinformatics Authors: Zhongxiao Li Antonella Napolitano Monica Fedele Xin Gao Francesco Napolitano Source Type: research
Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods
Methods Mol Biol. 2024 May 4. doi: 10.1007/7651_2024_541. Online ahead of print.ABSTRACTEpidermal stem cells, located in the skin, together with keratinocytes are transplanted in regenerative therapies, e.g., for the treatment of burns or other wounds. Here, we describe the protocol of their enzymatic isolation from human skin. It includes separation of the epidermis form the dermis by incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be seeded on collagen IV-coated dishes. The methods of analysis of epidermal stem cells markers (e.g., CD71, CD29)...
Source: Mol Biol Cell - May 3, 2024 Category: Molecular Biology Authors: Milena Deptu ła Ma łgorzata Zawrzykraj Paulina S łonimska Micha ł Pikuła Source Type: research
A Coculture System for Modeling Intestinal Epithelial-Fibroblast Crosstalk
Methods Mol Biol. 2024 May 4. doi: 10.1007/7651_2024_544. Online ahead of print.ABSTRACTEpithelial organoid monoculture is a powerful tool to model stem cell dynamics in vitro. However, extensive efforts have recently revealed various niche players and their significant roles in regulating epithelial stem cells. Among these niche components, fibroblasts have been heavily recognized in the field as a critical niche signal secretor. Thus, understanding the roles of fibroblasts in epithelial dynamics has become increasingly relevant and crucial. This propels the development of approaches to coculture epithelial 3D organoids w...
Source: Mol Biol Cell - May 3, 2024 Category: Molecular Biology Authors: Rebecca F Lee Mei-Lan Li Maria Figetakis Kaelyn Sumigray Source Type: research
Impact of HMGB1 on cancer development and therapeutic insights focused on CNS malignancy
Biochim Biophys Acta Rev Cancer. 2024 May 1:189105. doi: 10.1016/j.bbcan.2024.189105. Online ahead of print.ABSTRACTThe present study explores the complex roles of High Mobility Group Box 1 (HMGB1) in the context of cancer development, emphasizing glioblastoma (GBM) and other central nervous system (CNS) cancers. HMGB1, primarily known for its involvement in inflammation and angiogenesis, emerges as a multifaceted player in the tumorigenesis of GBM. The overexpression of HMGB1 correlates with glioma malignancy, influencing key pathways like RAGE/MEK/ERK and RAGE/Rac1. Additionally, HMGB1 secretion is linked to the maintena...
Source: Biochimica et Biophysica Acta - May 3, 2024 Category: Biochemistry Authors: Sucharita Patra Pritam Kumar Roy Ankita Dey Mahitosh Mandal Source Type: research
Application of stem cells in the study of developmental and functional toxicity of endodermal-derived organs caused by nanoparticles
This article discusses the use of stem cells to study the developmental and functional toxicity of organs of endodermal origin due to nanoparticles. The study discovered that various types of nanoparticles have varying effects on stem cells. The application of stem cell models can provide a possibility for studying the effects of nanoparticles on organ development and function, as they can more accurately reflect the toxic mechanisms of different types of nanoparticles. However, stem cell toxicology systems currently cannot fully reflect the effects of nanoparticles on entire organs. Therefore, the establishment of organoi...
Source: Toxicology in Vitro - May 3, 2024 Category: Toxicology Authors: Mulati Julaiti Haoqiang Guo Tingting Cui Nadire Nijiati Pengfei Huang Bowen Hu Source Type: research
Extracellular release of mitochondria induced by pre-hematopoietic stem cell transplant conditioning exacerbates GVHD
Blood Adv. 2024 May 3:bloodadvances.2023012328. doi: 10.1182/bloodadvances.2023012328. Online ahead of print.ABSTRACTDespite therapeutic advancements, GVHD is a major complication of HSCT. In current models of GVHD, tissue injury induced by cytotoxic conditioning regimens, along with translocation of microbes expressing Pathogen Associated Molecular Patterns (PAMPs), result in activation of host antigen-presenting cells (APC) to stimulate alloreactive donor T lymphocytes. Recent studies have demonstrated that in many pathologic states, tissue injury results in the release of mitochondria from the cytoplasm to the extracell...
Source: Adv Data - May 3, 2024 Category: Epidemiology Authors: Vijith Vijayan Hao Yan Juliane K Lohmeyer Kaylin A Prentiss Rachana V Patil Giulia Barbarito Ivan Lopez Aly Elezaby Kolten Peterson Jeanette Baker Nicolai P Ostberg Alice Bertaina Robert S Negrin Daria Mochly-Rosen Kenneth I Weinberg Bereketeab Haileselas Source Type: research
