Unraveling DEHP influence on hemoglobin S polymerization in sickle cell disease: Ex vivo, in vitro and in silico analysis
This study aimed to unravel DEHP effect on SCD patient's hemoglobin function. We found that HbS polymerization using whole erythrocytes is decreased by DEHP in ex vivo experiments and this effect might be mediated by the DEHP-VAL6 interaction, evaluated in silico. Isolated HbS exhibited less polymerization at low DEHP concentrations and increased polymerization rate at higher concentration. When analyzing the propensity to aggregate, HbS is more inclined to aggregate when compared to HbA due to the residue 6 mutation. Circular dichroism showed characteristic hemoglobin peaks for oxygenated HbS that are lost when oxygen is ...
Source: Toxicology in Vitro - April 23, 2024 Category: Toxicology Authors: Rodrigo Abreu Camacho Aghata Vitoria Machado Fernanda de Oliveira Mendon ça Lyzes Rosa Teixeira-Alves Camila Cristina Guimar ães-Nobre Evelyn Mendon ça-Reis Priscila Ferreira da Silva Thyago R Cardim-Pires Leandro Miranda-Alves Clemilson Berto-Junior Source Type: research
Effects of an environmentally relevant mixture of organochlorine pesticide compounds on adipogenesis and adipocyte function in an immortalized human adipocyte model
Toxicol In Vitro. 2024 Apr 20:105831. doi: 10.1016/j.tiv.2024.105831. Online ahead of print.ABSTRACTExposure to persistent organic pollutants (POPs), including organochlorine (OC) pesticide POPs, has been associated with the increased prevalence of obesity and type 2 diabetes. However, the underlying mechanisms through which exposure to these compounds may promote obesity and metabolic dysfunction remain an area of active investigation. To this end, the concentration dependent effects of an environmentally relevant mixture of OC pesticide POPs on adipocyte function was explored utilizing a translationally relevant immortal...
Source: Toxicology in Vitro - April 22, 2024 Category: Toxicology Authors: George E Howell Darian Young Source Type: research
In vitro evaluation of doxorubicin release from diopside particles on MG-63 and HF spheroids as a 3D model of tumor and healthy tissues
Toxicol In Vitro. 2024 Apr 17;98:105830. doi: 10.1016/j.tiv.2024.105830. Online ahead of print.ABSTRACTLocal drug delivery systems based on bioceramics ensure safe and effective treatment of bone defects and anticancer therapy. A promising drug delivery scaffold material for bone treatment applications is diopside (CaMgSi2O6) which is bioactive, degradable, and possesses drug-release ability. Currently, in vitro assessment of drug release from biomaterials is performed mostly on a 2D cell monolayer. However, to interpret and integrate biochemical signals, cells need a 3D microenvironment that provides cell-cell and cell-ex...
Source: Toxicology in Vitro - April 19, 2024 Category: Toxicology Authors: Inna N Bulygina Saida Sh Karshieva Elizaveta S Permyakova Artem A Korol Evgeny A Kolesnikov Rajan Choudhary Fedor S Senatov Elizaveta V Koudan Source Type: research
Substitution of bridge carbons for sulphur in calix[4]arene-bis- α-hydroxymethylphosphonic acid transformed mobile carrier into ionic channel accompanied with evoked muscle contraction and impaired neurotransmission powered by membrane action of resulting thiocalix[4]arene-bis-α-hydroxymethylphosphonic acid
Toxicol In Vitro. 2024 Apr 16:105815. doi: 10.1016/j.tiv.2024.105815. Online ahead of print.ABSTRACTThe action of calix[4]arenes C-424, C-425 and C-1193 has been investigated on suspended cholesterol/egg phosphatidylcholine lipid bilayer in a voltage-clamp mode. Comparative analysis with the membrane action by calix[4]arene-bis-α-hydroxymethylphosphonic acid (C-99) has shown that the substitution of bridge carbons for sulphur and addition of another methyl group to two alkyl tales in the lower rim of former dipropoxycalix[4]arene C-99 transformed mobile carrier that C-99 created in lipid bilayer (Shatursky et al., 2014) i...
Source: Toxicology in Vitro - April 18, 2024 Category: Toxicology Authors: Oleg Ya Shatursky Natalia V Krisanova Natalia Pozdnyakova Artem O Pastukhov Marina Dudarenko Lilia Kalynovska Alexander A Shkrabak Tatiana O Veklich Anna I Selikhova Serhii O Cherenok Tatiana A Borisova Vitaly I Kalchenko Sergyi O Kosterin Source Type: research
Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM < sub > 2.5 < /sub > on human lung cells
Toxicol In Vitro. 2024 Apr 16;98:105828. doi: 10.1016/j.tiv.2024.105828. Online ahead of print.ABSTRACTFine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season a...
Source: Toxicology in Vitro - April 15, 2024 Category: Toxicology Authors: Yaqian Wei Yan Chen Youwei Hong Jinsheng Chen Hong-Bo Li Hanhan Li Xuewen Yao Tariq Mehmood Xinyuan Feng Xiao-San Luo Source Type: research
Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM < sub > 2.5 < /sub > on human lung cells
Toxicol In Vitro. 2024 Apr 13:105828. doi: 10.1016/j.tiv.2024.105828. Online ahead of print.ABSTRACTFine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season and ...
Source: Toxicology in Vitro - April 15, 2024 Category: Toxicology Authors: Yaqian Wei Yan Chen Youwei Hong Jinsheng Chen Hong-Bo Li Hanhan Li Xuewen Yao Tariq Mehmood Xinyuan Feng Xiao-San Luo Source Type: research
Comparative in vitro toxicological effects of water-soluble and insoluble components of atmospheric PM < sub > 2.5 < /sub > on human lung cells
Toxicol In Vitro. 2024 Apr 13:105828. doi: 10.1016/j.tiv.2024.105828. Online ahead of print.ABSTRACTFine particulates in city air significantly impact human health, but the hazardous compositional mechanisms are still unclear. Besides the toxicity of environmental PM2.5 to in vitro human lung epithelial cells (A549), the independent cytotoxicity of PM2.5-bound water-soluble (WS-PM2.5) and water-insoluble (WIS-PM2.5) fractions were also compared by cell viability, oxidative stress (reactive oxygen species, ROS), and inflammatory injury (IL-6 and TNF-α). The cytotoxicity of PM2.5 varied significantly by sampling season and ...
Source: Toxicology in Vitro - April 15, 2024 Category: Toxicology Authors: Yaqian Wei Yan Chen Youwei Hong Jinsheng Chen Hong-Bo Li Hanhan Li Xuewen Yao Tariq Mehmood Xinyuan Feng Xiao-San Luo Source Type: research
Effects of iron oxide nanoparticles on the gene expression profiles of cerebral endotheliocytes and astrocytes
Toxicol In Vitro. 2024 Apr 12:105829. doi: 10.1016/j.tiv.2024.105829. Online ahead of print.ABSTRACTIron oxide nanoparticles (IONPs) are considered as the most biocompatible magnetic materials suitable for biomedical applications. Nevertheless, there are many evidences of their toxicity for living organisms and partially neurotoxicity. The central nervous system is protected from undesirable substances circulating in the bloodstream by the blood-brain barrier (BBB). And even if being small enough, some nanoparticles could be able to penetrate cell membranes in other cells but will often be delayed by the BBB cells. However...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Anna Tyumentseva Elena Khilazheva Valeria Petrova Sergey Stolyar Source Type: research
Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures
In conclusion, these data confirm the versatile use of iPSC differentiated cell types as standardizable and relevant model systems for in vitro toxicology.PMID:38615723 | DOI:10.1016/j.tiv.2024.105826 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Vidya Chandrasekaran Sara Wellens Aurore Bourguignon Ivo Djidrovski Leonie Fransen Sreya Ghosh Zahra Mazidi Cormac Murphy Carolina Nunes Pranika Singh Melinda Zana Lyle Armstrong Andr ás Dinnyés Johannes Grillari Regina Grillari-Voglauer Martin O Leonar Source Type: research
Risk assessment of Benzene, Toluene, Ethyl benzene, and Xylene (BTEX) in the atmospheric air around the world: A review
Toxicol In Vitro. 2024 Apr 12:105825. doi: 10.1016/j.tiv.2024.105825. Online ahead of print.ABSTRACTVolatile organic compounds, such as BTEX, have been the subject of numerous debates due to their detrimental effects on the environment and human health. Human beings have had a significant role in the emergence of this situation. Even though US EPA, WHO, and other health-related organizations have set standard limits as unhazardous levels, it has been observed that within or even below these limits, constant exposure to these toxic chemicals results in negative consequences as well. According to these facts, various studies...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Mohammad Ali Zahed Samira Salehi Mahtab Akbarzadeh Khoei Pedram Esmaeili Leila Mohajeri Source Type: research
Effects of iron oxide nanoparticles on the gene expression profiles of cerebral endotheliocytes and astrocytes
Toxicol In Vitro. 2024 Apr 12:105829. doi: 10.1016/j.tiv.2024.105829. Online ahead of print.ABSTRACTIron oxide nanoparticles (IONPs) are considered as the most biocompatible magnetic materials suitable for biomedical applications. Nevertheless, there are many evidences of their toxicity for living organisms and partially neurotoxicity. The central nervous system is protected from undesirable substances circulating in the bloodstream by the blood-brain barrier (BBB). And even if being small enough, some nanoparticles could be able to penetrate cell membranes in other cells but will often be delayed by the BBB cells. However...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Anna Tyumentseva Elena Khilazheva Valeria Petrova Sergey Stolyar Source Type: research
Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures
In conclusion, these data confirm the versatile use of iPSC differentiated cell types as standardizable and relevant model systems for in vitro toxicology.PMID:38615723 | DOI:10.1016/j.tiv.2024.105826 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Vidya Chandrasekaran Sara Wellens Aurore Bourguignon Ivo Djidrovski Leonie Fransen Sreya Ghosh Zahra Mazidi Cormac Murphy Carolina Nunes Pranika Singh Melinda Zana Lyle Armstrong Andr ás Dinnyés Johannes Grillari Regina Grillari-Voglauer Martin O Leonar Source Type: research
Risk assessment of Benzene, Toluene, Ethyl benzene, and Xylene (BTEX) in the atmospheric air around the world: A review
Toxicol In Vitro. 2024 Apr 12:105825. doi: 10.1016/j.tiv.2024.105825. Online ahead of print.ABSTRACTVolatile organic compounds, such as BTEX, have been the subject of numerous debates due to their detrimental effects on the environment and human health. Human beings have had a significant role in the emergence of this situation. Even though US EPA, WHO, and other health-related organizations have set standard limits as unhazardous levels, it has been observed that within or even below these limits, constant exposure to these toxic chemicals results in negative consequences as well. According to these facts, various studies...
Source: Toxicology in Vitro - April 14, 2024 Category: Toxicology Authors: Mohammad Ali Zahed Samira Salehi Mahtab Akbarzadeh Khoei Pedram Esmaeili Leila Mohajeri Source Type: research
Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations
In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the...
Source: Toxicology in Vitro - April 13, 2024 Category: Toxicology Authors: Lara Barroso Brito Carolina Motter Catarino Maria Claudia Passos Artur C Garcia Silva Desiree Cigaran Schuck Andrezza Di Pietro Micali Canavez Marize Campos Valadares Source Type: research
Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations
In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the...
Source: Toxicology in Vitro - April 13, 2024 Category: Toxicology Authors: Lara Barroso Brito Carolina Motter Catarino Maria Claudia Passos Artur C Garcia Silva Desiree Cigaran Schuck Andrezza Di Pietro Micali Canavez Marize Campos Valadares Source Type: research
