Ultraviolet Light-Emitting Diode Radiation Kills Leukemia Cells Without Affecting Hematopoiesis of Hematopoietic Stem Cells in Mice
CONCLUSIONS: Our results suggest that ultraviolet LED radiation can suppress the proliferative and tumorigenic abilities of leukemia cells without reducing the hematopoietic reconstitution capacity of hematopoietic stem cells, serving as a promising approach to kill leukemia cells in autograft before autologous hematopoietic stem cell transplant.PMID:38695592 | DOI:10.6002/ect.2023.0339 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - May 2, 2024 Category: Transplant Surgery Authors: Dong Xie Lirong Sun Xingqing Guo Jian Jiang Yan Sun Jiandong Sun Source Type: research
A new strategy for the treatment of middle ear infection using ciprofloxacin/amoxicillin-loaded ethyl cellulose/polyhydroxybutyrate nanofibers
In this study, excellent biocompatible ethyl cellulose and biodegradable polyhydroxybutyrate (PHB) biopolymer were used to fabricate drug-loaded nanofiber scaffolds using an electrospinning technique to overcome antibiotic overdose and insufficient efficacy of drug release during treatment. PHB polymer was produced from Halomonas sp., and the purity of PHB was found to around be 90 %. Additionally, ciprofloxacin and amoxicillin are highly preferable since both drugs are highly effective against gram-negative and gram-positive bacteria to treat several infections. Obtained smooth nanofibers were between 116.24 and 171.82 nm...
Source: International Journal of Biological Macromolecules - May 2, 2024 Category: Biochemistry Authors: Hatice Karabulut Dingli Xu Yuxi Ma Tufan Arslan Tut Songul Ulag Orkun Pinar Dilek Kazan Mehmet Mucahit Guncu Ali Sahin Hua Wei Jing Chen Oguzhan Gunduz Source Type: research
From islet transplantation to beta-cell regeneration: an update on beta-cell-based therapeutic approaches in type 1 diabetes
Expert Rev Endocrinol Metab. 2024 May 1:1-11. doi: 10.1080/17446651.2024.2347263. Online ahead of print.ABSTRACTINTRODUCTION: Type 1 diabetes (T1D) mellitus is an autoimmune disease in which immune cells, predominantly effector T cells, destroy insulin-secreting beta-cells. Beta-cell destruction led to various consequences ranging from retinopathy and nephropathy to neuropathy. Different strategies have been developed to achieve normoglycemia, including exogenous glucose compensation, whole pancreas transplantation, islet transplantation, and beta-cell replacement.AREAS COVERED: The last two decades of experience have show...
Source: Expert Review of Endocrinology and Metabolism - May 2, 2024 Category: Endocrinology Authors: Asef Azad Hasan Ali Altunbas Ayse Esra Manguoglu Source Type: research
Daratumumab and antineoplastic therapy versus antineoplastic therapy only for adults with newly diagnosed multiple myeloma ineligible for transplant
CONCLUSIONS: Overall analysis of four studies showed a potential benefit for daratumumab in terms of overall survival and progression-free survival and a slight potential benefit in quality of life. Participants treated with daratumumab probably experience increased serious adverse events. There were likely no differences between groups in adverse events (CTCAE grade ≥ 3); however, there are probably more infections (CTCAE grade ≥ 3) in participants treated with daratumumab. We identified six ongoing studies which might strengthen the certainty of evidence in a future update of this review.PMID:38695605 | DOI:10.1002/1...
Source: Cochrane Database of Systematic Reviews - May 2, 2024 Category: General Medicine Authors: Peter Langer Lukas John Ina Monsef Christof Scheid Vanessa Piechotta Nicole Skoetz Source Type: research
Outcomes and genetic dynamics of acute myeloid leukemia at first relapse
Haematologica. 2024 May 2. doi: 10.3324/haematol.2024.285057. Online ahead of print.ABSTRACTPatients with relapsed acute myeloid leukemia (rAML) experience dismal outcomes. We performed a comprehensive analysis of patients with rAML to determine the genetic dynamics and survival predictive factors. We analyzed 875 patients with newly diagnosed AML who received intensive treatment (IT) or low-intensity treatment (LIT). Of these patients, 197 experienced subsequent rAML. Data was available for 164 patients, with a median time from CR/CRi to relapse of 6.5 months. Thirty-five of the 164 patients (21%) experienced relapse afte...
Source: Haematologica - May 2, 2024 Category: Hematology Authors: Alex Bataller Hagop Kantarjian Alexandre Bazinet Tapan Kadia Naval Daver Courtney D DiNardo Gautam Borthakur Sanam Loghavi Keyur Patel Guilin Tang Koji Sasaki Nicholas J Short Musa Yilmaz Ghayas C Issa Yesid Alvarado Guillermo Montalban-Bravo Abhishek Mai Source Type: research
Use of iPSC-Derived Smooth Muscle Cells to Model Physiology and Pathology
Arterioscler Thromb Vasc Biol. 2024 May 2. doi: 10.1161/ATVBAHA.123.319703. Online ahead of print.ABSTRACTThe implementation of human-induced pluripotent stem cell models has introduced an additional tool for identifying molecular mechanisms of disease that complement animal models. Patient-derived or CRISPR/Cas9-edited induced pluripotent stem cells differentiated into smooth muscle cells (SMCs) have been leveraged to discover novel mechanisms, screen potential therapeutic strategies, and model in vivo development. The field has evolved over almost 15 years of research using human-induced pluripotent stem cell-SMCs and ha...
Source: Arteriosclerosis, Thrombosis and Vascular Biology - May 2, 2024 Category: Cardiology Authors: Callie S Kwartler Jose Emiliano Esparza Pinelo Source Type: research
Remodeling ceramide homeostasis promotes functional maturation of human pluripotent stem cell-derived β cells
Cell Stem Cell. 2024 Apr 26:S1934-5909(24)00137-1. doi: 10.1016/j.stem.2024.04.010. Online ahead of print.ABSTRACTHuman pluripotent stem cell-derived β cells (hPSC-β cells) show the potential to restore euglycemia. However, the immature functionality of hPSC-β cells has limited their efficacy in application. Here, by deciphering the continuous maturation process of hPSC-β cells post transplantation via single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq), we show that functional maturation of hPSC-β cells is an orderly multistep process during which ...
Source: Cell Stem Cell - May 2, 2024 Category: Stem Cells Authors: Huijuan Hua Yaqi Wang Xiaofeng Wang Shusen Wang Yunlu Zhou Yinan Liu Zhen Liang Huixia Ren Sufang Lu Shuangshuang Wu Yong Jiang Yue Pu Xiang Zheng Chao Tang Zhongyang Shen Cheng Li Yuanyuan Du Hongkui Deng Source Type: research
Raman Spectroscopy Analysis of Human Amniotic Fluid Cells from fetuses with Myelomeningocele
Exp Cell Res. 2024 Apr 30:114048. doi: 10.1016/j.yexcr.2024.114048. Online ahead of print.ABSTRACTPrenatal surgery for the treatment of spina bifida (myelomeningocele, MMC) significantly enhances the neurological prognosis of the patient. To ensure better protection of the spinal cord by large defects, the application of skin grafts produced with cells gained from the amniotic fluid is presently studied. In order to determine the most appropriate cells for this purpose, we tried to shed light on the extremely complex amniotic fluid cellular composition in healthy and MMC pregnancies. We exploited the potential of micro-Ram...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Luca Pontiggia Katarzyna Michalak-Micka Nadine H ürlimann Hesham K Yosef Roland B öni Agnes S Klar Martin Ehrbar Nicole Ochsenbein-K ölble Thomas Biedermann Ueli Moehrlen Source Type: research
The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells
CONCLUSION: Our findings reveal the effectiveness of N-Ad-MSCs in differentiating into IPCs in vitro and controlling the hyperglycemia of STZ-induced diabetic rats in vivo compared to DM-Ad-MSCs.PMID:38698488 | DOI:10.1186/s40659-024-00502-4 (Source: Cell Research)
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Omar I Badr Mohamed M Kamal Shohda A El-Maraghy Heba R Ghaiad Source Type: research
Human umbilical cord mesenchymal stem cells promote steroid-induced osteonecrosis of the femoral head repair by improving microvascular endothelial cell function
Aging (Albany NY). 2024 Apr 29;16. doi: 10.18632/aging.205794. Online ahead of print.ABSTRACTRecently, there has been growing interest in using cell therapy through core decompression (CD) to treat osteonecrosis of the femoral head (ONFH). Our study aimed to investigate the effectiveness and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in treating steroid-induced ONFH. We constructed a steroid-induced ONFH rabbit model as well as dexamethasone (Dex)-treated bone microvascular endothelial cells (BMECs) model of human femoral head. We injected hUCMSCs into the rabbit femoral head via CD. The effects of ...
Source: Aging - May 2, 2024 Category: Biomedical Science Authors: Junwen Chen Wenyi Jin Changheng Zhong Wenxiang Cai Liangkun Huang Jianlin Zhou Hao Peng Source Type: research
Raman Spectroscopy Analysis of Human Amniotic Fluid Cells from fetuses with Myelomeningocele
Exp Cell Res. 2024 Apr 30:114048. doi: 10.1016/j.yexcr.2024.114048. Online ahead of print.ABSTRACTPrenatal surgery for the treatment of spina bifida (myelomeningocele, MMC) significantly enhances the neurological prognosis of the patient. To ensure better protection of the spinal cord by large defects, the application of skin grafts produced with cells gained from the amniotic fluid is presently studied. In order to determine the most appropriate cells for this purpose, we tried to shed light on the extremely complex amniotic fluid cellular composition in healthy and MMC pregnancies. We exploited the potential of micro-Ram...
Source: Experimental Cell Research - May 2, 2024 Category: Cytology Authors: Luca Pontiggia Katarzyna Michalak-Micka Nadine H ürlimann Hesham K Yosef Roland B öni Agnes S Klar Martin Ehrbar Nicole Ochsenbein-K ölble Thomas Biedermann Ueli Moehrlen Source Type: research
Role of Hedgehog Signaling Pathways in Multipotent Mesenchymal Stem Cells Differentiation
Cell Transplant. 2024 Jan-Dec;33:9636897241244943. doi: 10.1177/09636897241244943.ABSTRACTMultipotent mesenchymal stem cells (MSCs) have high self-renewal and multi-lineage differentiation potentials and low immunogenicity, so they have attracted much attention in the field of regenerative medicine and have a promising clinical application. MSCs originate from the mesoderm and can differentiate not only into osteoblasts, cartilage, adipocytes, and muscle cells but also into ectodermal and endodermal cell lineages across embryonic layers. To design cell therapy for replacement of damaged tissues, it is essential to understa...
Source: Cell Transplantation - May 2, 2024 Category: Cytology Authors: Mengyu Wu Junwei Mi Guo-Xin Qu Shu Zhang Yi Jian Chu Gao Qingli Cai Jing Liu Jianxin Jiang Hong Huang Source Type: research
Improved therapeutic effects on vascular intimal hyperplasia by mesenchymal stem cells expressing MIR155HG that function as a ceRNA for microRNA-205
J Cell Mol Med. 2024 May;28(9):e18351. doi: 10.1111/jcmm.18351.ABSTRACTCoronary artery bypass grafting (CABG) is an effective treatment for coronary heart disease, with vascular transplantation as the key procedure. Intimal hyperplasia (IH) gradually leads to vascular stenosis, seriously affecting the curative effect of CABG. Mesenchymal stem cells (MSCs) were used to alleviate IH, but the effect was not satisfactory. This work aimed to investigate whether lncRNA MIR155HG could improve the efficacy of MSCs in the treatment of IH and to elucidate the role of the competing endogenous RNA (ceRNA). The effect of MIR155HG on MS...
Source: Molecular Medicine - May 2, 2024 Category: Molecular Biology Authors: Xiao Bai Zaiwen Qi Chuanliang Cai Hao Song Guangmin Song Xin Zhao Source Type: research
A new strategy for the treatment of middle ear infection using ciprofloxacin/amoxicillin-loaded ethyl cellulose/polyhydroxybutyrate nanofibers
In this study, excellent biocompatible ethyl cellulose and biodegradable polyhydroxybutyrate (PHB) biopolymer were used to fabricate drug-loaded nanofiber scaffolds using an electrospinning technique to overcome antibiotic overdose and insufficient efficacy of drug release during treatment. PHB polymer was produced from Halomonas sp., and the purity of PHB was found to around be 90 %. Additionally, ciprofloxacin and amoxicillin are highly preferable since both drugs are highly effective against gram-negative and gram-positive bacteria to treat several infections. Obtained smooth nanofibers were between 116.24 and 171.82 nm...
Source: International Journal of Biological Macromolecules - May 2, 2024 Category: Biochemistry Authors: Hatice Karabulut Dingli Xu Yuxi Ma Tufan Arslan Tut Songul Ulag Orkun Pinar Dilek Kazan Mehmet Mucahit Guncu Ali Sahin Hua Wei Jing Chen Oguzhan Gunduz Source Type: research
Improved therapeutic effects on vascular intimal hyperplasia by mesenchymal stem cells expressing MIR155HG that function as a ceRNA for microRNA-205
J Cell Mol Med. 2024 May;28(9):e18351. doi: 10.1111/jcmm.18351.ABSTRACTCoronary artery bypass grafting (CABG) is an effective treatment for coronary heart disease, with vascular transplantation as the key procedure. Intimal hyperplasia (IH) gradually leads to vascular stenosis, seriously affecting the curative effect of CABG. Mesenchymal stem cells (MSCs) were used to alleviate IH, but the effect was not satisfactory. This work aimed to investigate whether lncRNA MIR155HG could improve the efficacy of MSCs in the treatment of IH and to elucidate the role of the competing endogenous RNA (ceRNA). The effect of MIR155HG on MS...
Source: J Cell Mol Med - May 2, 2024 Category: Molecular Biology Authors: Xiao Bai Zaiwen Qi Chuanliang Cai Hao Song Guangmin Song Xin Zhao Source Type: research
