Cofilin-1 participates in the hyperfunction of myeloid dendritic cells in patients with severe aplastic anaemia
J Cell Mol Med. 2022 May 17. doi: 10.1111/jcmm.17359. Online ahead of print.ABSTRACTCofilin-1 interacts with actin to regulate cell movement. The importance of cofilin-1 in immunity has been established, and its involvement in a number of autoimmune diseases has been confirmed. However, its role in severe aplastic anaemia (SAA) remains elusive. Thus, the aim of the current study was to investigate the role of cofilin-1 in patients with SAA. Flow cytometry, Western blotting and real-time quantitative reverse transcription-polymerase chain reaction were performed to detect the mRNA and protein expression of cofilin-1 in myel...
Source: J Cell Mol Med - May 17, 2022 Category: Molecular Biology Authors: Yingying Sun Yu Zhang Hong Yu Huaquan Wang Zonghong Shao Chunyan Liu Source Type: research

Combination of FOXD1 and Plk2: A novel biomarker for predicting unfavourable prognosis of colorectal cancer
J Cell Mol Med. 2022 May 17. doi: 10.1111/jcmm.17361. Online ahead of print.ABSTRACTColorectal cancer (CRC) is a worldwide disease with worse survival. Our objective is to identify previously unrecognized prognostic factors to better evaluate disease progression. Seven GEO datasets were collected and analysed using R software, followed by KEGG enrichment analysis and TFs network construction. LASSO-COX analysis was performed to select the most useful prognostic features. COX model was used to analyse prognostic factors associated with OS. The survival curve was constructed using Kaplan-Meier analysis. A Nomogram model was ...
Source: J Cell Mol Med - May 17, 2022 Category: Molecular Biology Authors: Yaping Zong Yiming Miao Wenchang Li Minhua Zheng Zhuoqing Xu Han Gao Wenqing Feng Zifeng Xu Jingkun Zhao Lifei Shen Aiguo Lu Source Type: research

Alternatively activated macrophages at the recipient site improve fat graft retention by promoting angiogenesis and adipogenesis
This study aimed to trace host-derived macrophages and to evaluate the effects of macrophage infiltration at the recipient site during the early stage on long-term fat graft retention. In our novel mouse model, all mice underwent simulated liposuction and were divided into 2 groups. The fat procurement plus grafting (Pro-Grafting) group was engrafted with prepared fat (0.3 ml). The pro-Grafting+M2 group was engrafted with prepared fat (0.3 ml) mixed with 1.0 × 106 GFP+M0 macrophages, and then, 2 ng IL-4 was injected into the grafts on Day 3. In addition, 1.0 × 106 GFP+M0 macrophages were injected into the tail vein for t...
Source: J Cell Mol Med - May 16, 2022 Category: Molecular Biology Authors: Ye Li Xinyao Chen Lin Liu Yunzi Chen Xin Bi Yuting Chen Jialiang Zou Zijue Wang Ziqing Dong Feng Lu Source Type: research

Alternatively activated macrophages at the recipient site improve fat graft retention by promoting angiogenesis and adipogenesis
This study aimed to trace host-derived macrophages and to evaluate the effects of macrophage infiltration at the recipient site during the early stage on long-term fat graft retention. In our novel mouse model, all mice underwent simulated liposuction and were divided into 2 groups. The fat procurement plus grafting (Pro-Grafting) group was engrafted with prepared fat (0.3 ml). The pro-Grafting+M2 group was engrafted with prepared fat (0.3 ml) mixed with 1.0 × 106 GFP+M0 macrophages, and then, 2 ng IL-4 was injected into the grafts on Day 3. In addition, 1.0 × 106 GFP+M0 macrophages were injected into the tail vein for t...
Source: J Cell Mol Med - May 16, 2022 Category: Molecular Biology Authors: Ye Li Xinyao Chen Lin Liu Yunzi Chen Xin Bi Yuting Chen Jialiang Zou Zijue Wang Ziqing Dong Feng Lu Source Type: research

A possible new activator of PI3K-Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarction
This study evaluated effect of Huayu Qutan Recipe (HQR) pretreatment on myocardial apoptosis induced by AMI by regulating mitochondrial function via PI3K/Akt/Bad pathway in rats with obesity. For in vivo experiments, 60 male rats were randomly divided into 6 groups: sham group, AMI group, AMI (obese) group, 4.5, 9.0 and 18.0 g/kg/d HQR groups. The models fed on HQR with different concentrations for 2 weeks before AMI. For in vitro experiments, the cardiomyocytes line (H9c2) was used. Cells were pretreated with palmitic acid (PA) for 24 h, then to build hypoxia model followed by HQR-containing serum for 24 h. Related indica...
Source: J Cell Mol Med - May 14, 2022 Category: Molecular Biology Authors: He Tai Yu-Jing Tong Rui Yu You Yu Si-Cheng Yao Ling-Bing Li Ye Liu Xiao-Zheng Cui Jin-Song Kuang Xian-Sheng Meng Xiao-Lin Jiang Source Type: research

MIF/SCL3A2 depletion inhibits the proliferation and metastasis of colorectal cancer cells via the AKT/GSK-3 β pathway and cell iron death
This study investigated the mechanisms of migration inhibitory factor (MIF) and solute carrier family 3 member 2 (SLC3A2) in colorectal cancer progression. The levels of MIF and SLC3A2 expression in cells were measured by RT-qPCR. SW480 and SW620 cells were transfected with sh-MIF and sh-SLC3A2, respectively. MIF, SLC3A2, GPX4, E-cadherin and N-cadherin expression were detected by immunofluorescence (IF). CCK8 and Transwell assays were performed to detect cell proliferation and migration. Co-immunoprecipitation (CoIP) was used to measure the binding activity of MIF and SLC3A2. Finally, a nude mouse tumorigenicity assay was...
Source: J Cell Mol Med - May 14, 2022 Category: Molecular Biology Authors: Guan Huang Lili Ma Lan Shen Yan Lei Lili Guo Yongjian Deng Yanqing Ding Source Type: research

A possible new activator of PI3K-Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarction
This study evaluated effect of Huayu Qutan Recipe (HQR) pretreatment on myocardial apoptosis induced by AMI by regulating mitochondrial function via PI3K/Akt/Bad pathway in rats with obesity. For in vivo experiments, 60 male rats were randomly divided into 6 groups: sham group, AMI group, AMI (obese) group, 4.5, 9.0 and 18.0 g/kg/d HQR groups. The models fed on HQR with different concentrations for 2 weeks before AMI. For in vitro experiments, the cardiomyocytes line (H9c2) was used. Cells were pretreated with palmitic acid (PA) for 24 h, then to build hypoxia model followed by HQR-containing serum for 24 h. Related indica...
Source: J Cell Mol Med - May 14, 2022 Category: Molecular Biology Authors: He Tai Yu-Jing Tong Rui Yu You Yu Si-Cheng Yao Ling-Bing Li Ye Liu Xiao-Zheng Cui Jin-Song Kuang Xian-Sheng Meng Xiao-Lin Jiang Source Type: research

MIF/SCL3A2 depletion inhibits the proliferation and metastasis of colorectal cancer cells via the AKT/GSK-3 β pathway and cell iron death
This study investigated the mechanisms of migration inhibitory factor (MIF) and solute carrier family 3 member 2 (SLC3A2) in colorectal cancer progression. The levels of MIF and SLC3A2 expression in cells were measured by RT-qPCR. SW480 and SW620 cells were transfected with sh-MIF and sh-SLC3A2, respectively. MIF, SLC3A2, GPX4, E-cadherin and N-cadherin expression were detected by immunofluorescence (IF). CCK8 and Transwell assays were performed to detect cell proliferation and migration. Co-immunoprecipitation (CoIP) was used to measure the binding activity of MIF and SLC3A2. Finally, a nude mouse tumorigenicity assay was...
Source: J Cell Mol Med - May 14, 2022 Category: Molecular Biology Authors: Guan Huang Lili Ma Lan Shen Yan Lei Lili Guo Yongjian Deng Yanqing Ding Source Type: research

Inhibition of ferroptosis promotes megakaryocyte differentiation and platelet production
J Cell Mol Med. 2022 May 13. doi: 10.1111/jcmm.17289. Online ahead of print.NO ABSTRACTPMID:35560727 | DOI:10.1111/jcmm.17289 (Source: J Cell Mol Med)
Source: J Cell Mol Med - May 13, 2022 Category: Molecular Biology Authors: Baoquan Song Wenjing Miao Qingya Cui Bingyu Shi Jian Zhang Huiying Qiu Leisheng Zhang Yue Han Source Type: research

Research progress of endothelial-mesenchymal transition in diabetic kidney disease
J Cell Mol Med. 2022 May 13. doi: 10.1111/jcmm.17356. Online ahead of print.ABSTRACTRenal fibrosis is an important pathological feature of diabetic kidney disease (DKD), manifested as tubular interstitial fibrosis, tubular atrophy, glomerulosclerosis and damage to the normal structure of the kidney. Renal fibrosis can eventually develop into renal failure. A better understanding of renal fibrosis in DKD is needed due to clinical limitations of current anti-fibrotic drugs in terms of effectiveness, cost-effectiveness and side effects. Fibrosis is characterized by local excessive deposition of extracellular matrix, which is ...
Source: J Cell Mol Med - May 13, 2022 Category: Molecular Biology Authors: Ying Chen Hang Zou Hongwei Lu Hong Xiang Shuhua Chen Source Type: research

Targeted regulation of FoxO1 in chondrocytes prevents age-related osteoarthritis via autophagy mechanism
J Cell Mol Med. 2022 May 13. doi: 10.1111/jcmm.17319. Online ahead of print.ABSTRACTAutophagy is designated as a biological recycling process to maintain cellular homeostasis by the sequestration of damaged proteins and organelles in plasma and cargo delivery to lysosomes for degradation and reclamation. This organelle recycling process promotes chondrocyte homeostasis and has been previously implicated in osteoarthritis (OA). Autophagy is widely involved in regulating chondrocyte degeneration markers such as MMPs, ADAMSTs and Col10 in chondrocytes. The critical autophagy-related (ATG) proteins have now been considered the...
Source: J Cell Mol Med - May 13, 2022 Category: Molecular Biology Authors: Jiaji Yue Aikebaier Aobulikasimu Weichao Sun Shuyu Liu Wei Xie Wei Sun Source Type: research

Inhibition of TRPC4 channel activity in colonic myocytes by tricyclic antidepressants disrupts colonic motility causing constipation
J Cell Mol Med. 2022 May 12. doi: 10.1111/jcmm.17348. Online ahead of print.ABSTRACTTricyclic antidepressants (TCAs) have been used to treat depression and were recently approved for treating irritable bowel syndrome (IBS) patients with severe or refractory IBS symptoms. However, the molecular mechanism of TCA action in the gastrointestinal (GI) tract remains poorly understood. Transient receptor potential channel canonical type 4 (TRPC4), which is a Ca2+ -permeable nonselective cation channel, is a critical regulator of GI excitability. Herein, we investigated whether TCA modulates TRPC4 channel activity and which mechani...
Source: J Cell Mol Med - May 13, 2022 Category: Molecular Biology Authors: Byeongseok Jeong Tae Sik Sung Dongju Jeon Kyu Joo Park Jae Yeoul Jun Insuk So Chansik Hong Source Type: research

Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia
CONCLUSION: This comprehensive molecular profiling of brain samples from patients with VaD reveals previously unknown molecular changes in cells of the neurovascular niche, and an attempt at regeneration in injured white matter.PMID:35543222 | DOI:10.1111/jcmm.17315 (Source: J Cell Mol Med)
Source: J Cell Mol Med - May 11, 2022 Category: Molecular Biology Authors: Daniel N Mitroi Min Tian Riki Kawaguchi William E Lowry S Thomas Carmichael Source Type: research

Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia
CONCLUSION: This comprehensive molecular profiling of brain samples from patients with VaD reveals previously unknown molecular changes in cells of the neurovascular niche, and an attempt at regeneration in injured white matter.PMID:35543222 | DOI:10.1111/jcmm.17315 (Source: J Cell Mol Med)
Source: J Cell Mol Med - May 11, 2022 Category: Molecular Biology Authors: Daniel N Mitroi Min Tian Riki Kawaguchi William E Lowry S Thomas Carmichael Source Type: research

Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia
CONCLUSION: This comprehensive molecular profiling of brain samples from patients with VaD reveals previously unknown molecular changes in cells of the neurovascular niche, and an attempt at regeneration in injured white matter.PMID:35543222 | DOI:10.1111/jcmm.17315 (Source: J Cell Mol Med)
Source: J Cell Mol Med - May 11, 2022 Category: Molecular Biology Authors: Daniel N Mitroi Min Tian Riki Kawaguchi William E Lowry S Thomas Carmichael Source Type: research