LIX1 regulates YAP activity and controls gastrointestinal cancer cell plasticity.
ure S Abstract Gastrointestinal stromal tumours (GISTs), the most common mesenchymal neoplasm of the gastrointestinal tract, result from deregulated proliferation of transformed KIT-positive interstitial cells of Cajal that share mesenchymal progenitors with smooth muscle cells. Despite the identification of selective KIT inhibitors, primary resistance and relapse remain a major concern. Moreover, most patients develop resistance partly through reactivation of KIT and its downstream signalling pathways. We previously identified the Limb Expression 1 (LIX1) gene as a unique marker of digestive mesenchyme immaturity...
Source: J Cell Mol Med - July 7, 2020 Category: Molecular Biology Authors: Guérin A, Martire D, Trenquier E, Lesluyes T, Sagnol S, Pratlong M, Lefebvre E, Chibon F, de Santa Barbara P, Faure S Tags: J Cell Mol Med Source Type: research

Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.
In this study, db/db mice and C2C12 myoblasts treated by high glucose and palmitate and oleate were chose as animal and cellular models. We explored how exogenous H2 S attenuated the degradation of skeletal muscle via the modification of MuRF1 S-sulfhydration in db/db mice. Our results show cystathionine-r-lyase expression, and H2 S level in skeletal muscle of db/db mice was reduced. Simultaneously, exogenous H2 S could alleviate ROS production and reverse expression of ER stress protein markers. Exogenous H2 S could decrease the ubiquitination level of MYOM1 and MYH4 in db/db mice. In addition, exogenous H2 S reduced the ...
Source: J Cell Mol Med - July 7, 2020 Category: Molecular Biology Authors: Lu F, Lu B, Zhang L, Wen J, Wang M, Zhang S, Li Q, Shu F, Sun Y, Liu N, Peng S, Zhao Y, Dong S, Zhao D, Lu F, Zhang W Tags: J Cell Mol Med Source Type: research

Positive feedback in Cav-1-ROS signalling in PSCs mediates metabolic coupling between PSCs and tumour cells.
In this study, we aimed to investigate the effect of pancreatic stellate cell (PSC) Cav-1 on PDAC metabolism and aggression. We found that Cav-1 is expressed at low levels in PDAC stroma and that the loss of stromal Cav-1 is associated with poor survival. In PSCs, knockdown of Cav-1 promoted the production of reactive oxygen species (ROS), while ROS production further reduced the expression of Cav-1. Positive feedback occurs in Cav-1-ROS signalling in PSCs, which promotes PDAC growth and induces stroma-tumour metabolic coupling in PDAC. In PSCs, positive feedback in Cav-1-ROS signalling induced a shift in energy metabolism...
Source: J Cell Mol Med - July 7, 2020 Category: Molecular Biology Authors: Shao S, Qin T, Qian W, Yue Y, Xiao Y, Li X, Zhang D, Wang Z, Ma Q, Lei J Tags: J Cell Mol Med Source Type: research

Spirulina platensis alleviates chronic inflammation with modulation of gut microbiota and intestinal permeability in rats fed a high-fat diet.
In this study, rats were fed a high-fat diet fed with 3% S platensis for 14 weeks. We analysed endotoxin, the composition of the microbiota, inflammation and gut permeability. We found that S platensis decreased the bodyweight and visceral fat pads weight of the HFD-fed rats. In addition, it lowered the levels of lipopolysaccharide and pro-inflammatory cytokines in serum. Our results showed that S platensis could largely reduce the relative amount of Proteobacteria and the Firmicutes/Bacteroidetes ratio in faecal samples from HFD-fed rats. S platensis significantly reduced intestinal inflammation, ...
Source: J Cell Mol Med - July 7, 2020 Category: Molecular Biology Authors: Yu T, Wang Y, Chen X, Xiong W, Tang Y, Lin L Tags: J Cell Mol Med Source Type: research

Autophagy regulation is an effective strategy to improve the prognosis of chemically induced acute liver injury based on experimental studies.
Abstract Acute liver injury (ALI) induced by chemicals in current experimental studies is characterized by inflammation, oxidative stress and necrosis, which can greatly influence the long-term outcome and lead to liver failure. In liver cells, different autophagy forms envelop cytoplasm components, including proteins, endoplasmic reticulum (ER), mitochondria and lipids, and they effectively participate in breaking down the cargo enclosed inside lysosomes to replenish cellular energy and contents. In general, autophagy serves as a cell survival mechanism in stressful microenvironments, but it also serves as a dest...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Hu C, Zhao L, Shen M, Wu Z, Li L Tags: J Cell Mol Med Source Type: research

D1 receptor-mediated endogenous tPA upregulation contributes to blood-brain barrier injury after acute ischaemic stroke.
Abstract Blood-brain barrier (BBB) integrity injury within the thrombolytic time window is becoming a critical target to reduce haemorrhage transformation (HT). We have previously reported that BBB damage was initially damaged in non-infarcted striatum after acute ischaemia stroke. However, the underlying mechanism is not clear. Since acute ischaemic stroke could induce a significant increase of dopamine release in striatum, in current study, our aim is to investigate the role of dopamine receptor signal pathway in BBB integrity injury after acute ischaemia using rat middle cerebral artery occlusion model. Our dat...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Wang Y, Wang X, Zhang X, Chen S, Sun Y, Liu W, Jin X, Zheng G Tags: J Cell Mol Med Source Type: research

Mesenchymal to epithelial transition driven by canine distemper virus infection of canine histiocytic sarcoma cells contributes to a reduced cell motility in vitro.
This study hypothesizes that a CDV infection of canine histiocytic sarcoma cells (DH82 cells) triggers the MET process leading to a decreased cellular motility. Immunofluorescence and immunoblotting were used to investigate the expression of epithelial and mesenchymal markers followed by scratch assay and an invasion assay as functional confirmation. Furthermore, microarray data were analysed for genes associated with the MET process, invasion and angiogenesis. CDV-infected cells exhibited an increased expression of epithelial markers such as E-cadherin and cytokeratin 8 compared to controls, indicating a MET process. This...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Armando F, Gambini M, Corradi A, Becker K, Marek K, Pfankuche VM, Mergani AE, Brogden G, de Buhr N, von Köckritz-Blickwede M, Naim HY, Baumgärtner W, Puff C Tags: J Cell Mol Med Source Type: research

Calpain-2 plays a pivotal role in the inhibitory effects of propofol against TNF- α-induced autophagy in mouse hippocampal neurons.
Calpain-2 plays a pivotal role in the inhibitory effects of propofol against TNF-α-induced autophagy in mouse hippocampal neurons. J Cell Mol Med. 2020 Jul 06;: Authors: Li Y, He Z, Lv H, Chen W, Chen J Abstract Calpains are calcium-dependent proteases and play critical roles in neuronal autophagy induced by inflammation. Propofol has been reported to exert anti-inflammatory effects in neurons. We aimed to identify whether and how propofol-modulated calpain activity and neuron autophagy in response to tumour necrosis factor-α (TNF-α). Mouse hippocampal neurons were pre-treated with p...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Li Y, He Z, Lv H, Chen W, Chen J Tags: J Cell Mol Med Source Type: research

Astragaloside IV alleviates mouse slow transit constipation by modulating gut microbiota profile and promoting butyric acid generation.
In this study, we examined the role of astragaloside IV in modulating mouse gut microbiota structure and the generation of SCFAs, as well as in slow transit constipation (STC). An STC model was established by treating mice with loperamide, in which the therapeutic effects of astragaloside IV were evaluated. The microbiota community structure and SCFA content were analysed by 16S rRNA gene sequencing and gas chromatography-mass spectrometry, respectively. The influence of butyrate on STC was assessed using a mouse model and Cajal cells (ICC). Astragaloside IV promoted defecation, improved intestinal mobility, suppressed ICC...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: He Q, Han C, Huang L, Yang H, Hu J, Chen H, Dou R, Ren D, Lin H Tags: J Cell Mol Med Source Type: research

Knock-out of MicroRNA 145 impairs cardiac fibroblast function and wound healing post-myocardial infarction.
Abstract Prevention of infarct scar thinning and dilatation and stimulation of scar contracture can prevent progressive heart failure. Since microRNA 145 (miR-145) plays an important role in cardiac fibroblast response to wound healing and cardiac repair after an myocardial infarction (MI), using a miR-145 knock-out (KO) mouse model, we evaluated contribution of down-regulation of miR-145 to cardiac fibroblast and myofibroblast function during adverse cardiac remodelling. Cardiac function decreased more and the infarct size was larger in miR-145 KO than that in WT mice after MI and this phenomenon was accompanied ...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Song HF, He S, Li SH, Wu J, Yin W, Shao Z, Du GQ, Wu J, Li J, Weisel RD, Verma S, Xie J, Li RK Tags: J Cell Mol Med Source Type: research

Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.
Abstract Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into a variety of cell types. Bortezomib, the first approved proteasome inhibitor used for the treatment of multiple myeloma (MM), has been shown to induce osteoblast differentiation, making it beneficial for myeloma bone disease. In the present study, we aimed to investigate the effects and underlying mechanisms of bortezomib on the cell cycle during osteogenic differentiation. We confirmed that low doses of bortezomib can induce MSCs towards osteogenic differentiation, but high doses are toxic. In the course of bortezomib-ind...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Zhang D, Fan R, Lei L, Lei L, Wang Y, Lv N, Chen P, Williamson RA, Wang B, Hu J Tags: J Cell Mol Med Source Type: research

Preclinical insights into the gut-skeletal muscle axis in chronic gastrointestinal diseases.
Abstract Muscle wasting represents a constant pathological feature of common chronic gastrointestinal diseases, including liver cirrhosis (LC), inflammatory bowel diseases (IBD), chronic pancreatitis (CP) and pancreatic cancer (PC), and is associated with increased morbidity and mortality. Recent clinical and experimental studies point to the existence of a gut-skeletal muscle axis that is constituted by specific gut-derived mediators which activate pro- and anti-sarcopenic signalling pathways in skeletal muscle cells. A pathophysiological link between both organs is also provided by low-grade systemic inflammatio...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Ehlers L, Bannert K, Rohde S, Berlin P, Reiner J, Wiese M, Doller J, Lerch MM, Aghdassi AA, Meyer F, Valentini L, Agrifoglio O, Metges CC, Lamprecht G, Jaster R Tags: J Cell Mol Med Source Type: research

PCSK9 inhibitor and atorvastatin reduce cardiac impairment in ovariectomized prediabetic rats via improved mitochondrial function and Ca2+ regulation.
Abstract Post-menopausal women have a higher risk of developing cardiometabolic dysfunction. Atorvastatin attenuates dyslipidaemia and cardiac dysfunction but it can have undesirable effects including increased risk of diabetes and myalgia. Currently, the proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor efficiently reduces low-density lipoprotein cholesterol (LDL-C) levels more effectively than atorvastatin. We have been suggested that PCSK9 inhibitor attenuated cardiometabolic impairment more effectively than atorvastatin in ovariectomized prediabetic rats. Female Wistar rats (n = 48) wer...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Amput P, Palee S, Arunsak B, Pratchayasakul W, Thonusin C, Kerdphoo S, Jaiwongkam T, Chattipakorn SC, Chattipakorn N Tags: J Cell Mol Med Source Type: research

Protective role of NRF2 in macrovascular complications of diabetes.
Abstract Macrovascular complications develop in over a half of the diabetic individuals, resulting in high morbidity and mortality. This poses a severe threat to public health and a heavy burden to social economy. It is therefore important to develop effective approaches to prevent or slow down the pathogenesis and progression of macrovascular complications of diabetes (MCD). Oxidative stress is a major contributor to MCD. Nuclear factor (erythroid-derived 2)-like 2 (NRF2) governs cellular antioxidant defence system by activating the transcription of various antioxidant genes, combating diabetes-induced oxidative ...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Wu J, Sun X, Jiang Z, Jiang J, Xu L, Tian A, Sun X, Meng H, Li Y, Huang W, Jia Y, Wu H Tags: J Cell Mol Med Source Type: research

Role of aldo-keto reductase family 1 member B1 (AKR1B1) in the cancer process and its therapeutic potential.
Abstract The role of aldo-keto reductase family 1 member B1 (AKR1B1) in cancer is not totally clear but growing evidence is suggesting to have a great impact on cancer progression. AKR1B1 could participate in a complicated network of signalling pathways, proteins and miRNAs such as mir-21 mediating mechanisms like inflammatory responses, cell cycle, epithelial to mesenchymal transition, cell survival and apoptosis. AKR1B1 has been shown to be mostly overexpressed in cancer. This overexpression has been associated with inflammatory mediators including nuclear factor kappa-light-chain-enhancer of activated B cells (...
Source: J Cell Mol Med - July 6, 2020 Category: Molecular Biology Authors: Khayami R, Hashemi SR, Kerachian MA Tags: J Cell Mol Med Source Type: research

WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.
In conclusion, WWP2 modulates hypertensive angiopathy by regulating SIRT1-STAT3 and WWP2 suppression in VSMCs can alleviate hypertensive angiopathy vitro and vivo. These findings provide new insights into the treatment of hypertensive vascular diseases. PMID: 32627301 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 5, 2020 Category: Molecular Biology Authors: Zhang Y, You S, Tian Y, Lu S, Cao L, Sun Y, Zhang N Tags: J Cell Mol Med Source Type: research

Ectodermal-neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/ β-catenin pathway.
Ectodermal-neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β-catenin pathway. J Cell Mol Med. 2020 Jul 03;: Authors: Zhou Y, Tang X, Niu L, Liu Y, Wang B, He J Abstract Breast cancer, as the most common malignancy, is the second leading cause of cancer-related death in women. One of the kelch family member ENC1 is involved in various pathophysiologic processes. But the role of ENC1 in breast cancer has not been investigated. The present study value the feature, clinical significance and the molecular mechanisms of ENC1 in breas...
Source: J Cell Mol Med - July 3, 2020 Category: Molecular Biology Authors: Zhou Y, Tang X, Niu L, Liu Y, Wang B, He J Tags: J Cell Mol Med Source Type: research

An in vitro model for hypertrophic adipocytes: Time-dependent adipocyte proteome and secretome changes under high glucose and high insulin conditions.
Abstract Obesity is the consequence of a positive energy balance and characterized by enlargement of the adipose tissue, which in part is due to hyperplasia and hypertrophy of the adipocytes. Not much is known about the transition of normal mature adipocytes to the hypertrophic state, which in vivo is very hard to study. Here, we have maintained mature human SGBS cells as a surrogate for adipocytes, changes of morphological and molecular metabolism of the adipocytes were monitored over the first 4 days and the last 4 days. In total, 393 cellular proteins and 246 secreted proteins were identified for furt...
Source: J Cell Mol Med - July 3, 2020 Category: Molecular Biology Authors: Qiao Q, Bouwman FG, Renes J, Mariman ECM Tags: J Cell Mol Med Source Type: research

CRISPR/Cas9-mediated whole genomic wide knockout screening identifies mitochondrial ribosomal proteins involving in oxygen-glucose deprivation/reperfusion resistance.
Abstract Recanalization therapy by intravenous thrombolysis or endovascular therapy is critical for the treatment of cerebral infarction. However, the recanalization treatment will also exacerbate acute brain injury and even severely threatens human life due to the reperfusion injury. So far, the underlying mechanisms for cerebral ischaemia-reperfusion injury are poorly understood and effective therapeutic interventions are yet to be discovered. Therefore, in the research, we subjected SK-N-BE(2) cells to oxygen-glucose deprivation/reperfusion (OGDR) insult and performed a pooled genome-wide CRISPR (clustered regu...
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Guan X, Zhang H, Qin H, Chen C, Hu Z, Tan J, Zeng L Tags: J Cell Mol Med Source Type: research

LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.
Abstract Lipase member H (LIPH), a novel member of the triglyceride lipase family. The clinical implications of its expression in breast cancer are still unclear. Therefore, in this study, we investigated the associations between LIPH and the tumorigenic behaviours of 144 triple-negative breast cancer (TNBC) patients. The ratio and mammosphere-forming ability of CD44+/CD24- stem-like cells were tested. The role of LIPH in breast cancer cell migration and invasion was also evaluated. In addition, the effect of LIPH silencing on mitochondrial respiration was determined using the Seahorse assay. Finally, the effect o...
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Zhang Y, Zhu X, Qiao X, Gu X, Xue J, Han Y, Sun L, Cui M, Liu C Tags: J Cell Mol Med Source Type: research

Dioscin elicits anti-tumour immunity by inhibiting macrophage M2 polarization via JNK and STAT3 pathways in lung cancer.
In conclusion, dioscin may act as a new anti-tumour agent by inhibiting TAMs via JNK and STAT3 pathways in lung cancer. PMID: 32618105 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Cui L, Yang G, Ye J, Yao Y, Lu G, Chen J, Fang L, Lu S, Zhou J Tags: J Cell Mol Med Source Type: research

Comprehensive analysis of autophagy-related prognostic genes in breast cancer.
Abstract Accumulating evidence revealed that autophagy played vital roles in breast cancer (BC) progression. Thus, the aim of this study was to investigate the prognostic value of autophagy-related genes (ARGs) and develop a ARG-based model to evaluate 5-year overall survival (OS) in BC patients. We acquired ARG expression profiling in a large BC cohort (N = 1007) from The Cancer Genome Atlas (TCGA) database. The correlation between ARGs and OS was confirmed by the LASSO and Cox regression analyses. A predictive model was established based on independent prognostic variables. Thus, time-dependent receive...
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Lai J, Chen B, Mok H, Zhang G, Ren C, Liao N Tags: J Cell Mol Med Source Type: research

Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice.
Abstract Mindin is important in broad spectrum of immune responses. On the other hand, we previously reported that mindin attenuated human colon cancer development by blocking angiogenesis through Egr-1-mediated regulation. However, the mice original mindin directly suppressed the syngenic colorectal cancer (CRC) growth in our recent study and we aimed to further define the role of mindin during CRC development in mice. We established the mouse syngeneic CRC CMT93 and CT26 WT cell lines with stable mindin knock-down or overexpression. These cells were also subcutaneously injected into C57BL/6 and BALB/c mice as we...
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Cheng XS, Huo YN, Fan YY, Xiao CX, Ouyang XM, Liang LY, Lin Y, Wu JF, Ren JL, Guleng B Tags: J Cell Mol Med Source Type: research

Association between METTL3 gene polymorphisms and neuroblastoma susceptibility: A nine-centre case-control study.
Abstract Neuroblastoma ranks as the most commonly seen and deadly solid tumour in infancy. The aberrant activity of m6 A-RNA methyltransferase METTL3 is involved in human cancers. Therefore, functional genetic variants in the METTL3 gene may contribute to neuroblastoma risk. In the current nine-centre case-control study, we aimed to analyse the association between the METTL3 gene single nucleotide polymorphisms (SNPs) and neuroblastoma susceptibility. We genotyped four METTL3 gene SNPs (rs1061026 T>G, rs1061027 C>A, rs1139130 A>G, and rs1263801 G>C) in 968 neuroblastoma patients and 1814 controls in Ch...
Source: J Cell Mol Med - July 2, 2020 Category: Molecular Biology Authors: Bian J, Zhuo Z, Zhu J, Yang Z, Jiao Z, Li Y, Cheng J, Zhou H, Li S, Li L, He J, Liu Y Tags: J Cell Mol Med Source Type: research

Glycation of fibronectin inhibits VEGF-induced angiogenesis by uncoupling VEGF receptor-2-c-Src crosstalk.
Abstract Glycation of extracellular matrix proteins has been demonstrated to contribute to the pathogenesis of vascular complications. However, no previous report has shown the role of glycated fibronectin (FN) in vascular endothelial growth factor (VEGF)-induced angiogenesis. Thus, this study aimed to investigate the effects of glycated FN on VEGF signalling and to clarify the molecular mechanisms involved. FN was incubated with methylglyoxal (MGO) in vitro to synthesize glycated FN, and human umbilical vein endothelial cells (HUVECs) were seeded onto unmodified and MGO-glycated FN. Then, VEGF-induced angiogenesi...
Source: J Cell Mol Med - July 1, 2020 Category: Molecular Biology Authors: Chen T, Dong J, Zhou H, Deng X, Li R, Chen N, Luo M, Li Y, Wu J, Wang L Tags: J Cell Mol Med Source Type: research

Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells.
Abstract In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to promote neurovascular repair. Autologous EPCs transfected to express specific therapeutic proteins offer an innovative therapeutic option. Here, we demonstrate that EPC transfection by electroporation with plasmids encoding the reporter protein GFP or an anti-β-amyloid antibody fragmen...
Source: J Cell Mol Med - July 1, 2020 Category: Molecular Biology Authors: Heller L, Thinard R, Chevalier M, Arpag S, Jing Y, Greferath R, Heller R, Nicolau C Tags: J Cell Mol Med Source Type: research

Development and validation of immune inflammation-based index for predicting the clinical outcome in patients with nasopharyngeal carcinoma.
In conclusion, the combined inflammation indicators were superior to single haematological indicator in the diagnosis and prognosis of NPC. These inflammation indicators can be used to supply the current evaluation system of the TNM staging system to help predict the prognosis in NPC patients. PMID: 32603520 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 30, 2020 Category: Molecular Biology Authors: Zeng X, Liu G, Pan Y, Li Y Tags: J Cell Mol Med Source Type: research

Neobavaisoflavone inhibits osteoclastogenesis through blocking RANKL signalling-mediated TRAF6 and c-Src recruitment and NF- κB, MAPK and Akt pathways.
In this study, applying comprehensive two-dimensional cell membrane chromatographic/C18 column/time-of-flight mass spectrometry (2D CMC/C18 column/TOFMS) system, neobavaisoflavone (NBIF), for the first time, was identified for the bioaffinity with RAW 264.7 cells membranes from the extracts of P corylifolia. Here, we revealed that NBIF inhibited RANKL-mediated osteoclastogenesis in bone marrow monocytes (BMMCs) and RAW264.7 cells dose dependently at the early stage. Moreover, NBIF inhibited osteoclasts function demonstrated by actin ring formation assay and pit-formation assay. With regard to the underlying molecular mecha...
Source: J Cell Mol Med - June 30, 2020 Category: Molecular Biology Authors: Chen H, Fang C, Zhi X, Song S, Gu Y, Chen X, Cui J, Hu Y, Weng W, Zhou Q, Wang Y, Wang Y, Jiang H, Li X, Cao L, Chen X, Su J Tags: J Cell Mol Med Source Type: research

Monocyte subsets in bone marrow grafts may contribute to a low incidence of acute graft-vs-host disease for young donors.
Abstract Young donors are associated with a lower cumulative incidence of acute graft-vs-host disease (aGVHD) after allogenic haematopoietic stem cell transplantation (allo-HSCT) than old donors. Although grafts are harvested from healthy donors, it is unclear whether donor age is associated with aGVHD occurrence owing to its effect on cell compositions in grafts. Moreover, the differences in monocyte subsets in grafts between young and old donors and the association between monocyte subsets in bone marrow (BM) grafts and aGVHD remain to be elucidated. In the current study, non-classical monocytes and the CD4+ /CD...
Source: J Cell Mol Med - June 30, 2020 Category: Molecular Biology Authors: Wen Q, Zhao HY, Yao WL, Zhang YY, Fu HX, Wang Y, Xu LP, Zhang XH, Kong Y, Huang XJ Tags: J Cell Mol Med Source Type: research

Salvianic acid A alleviates chronic alcoholic liver disease by inhibiting HMGB1 translocation via down-regulating BRD4.
Abstract Alcoholic liver disease (ALD) is the major cause of chronic liver disease and a global health concern. ALD pathogenesis is initiated with liver steatosis, and ALD can progress to steatohepatitis, fibrosis, cirrhosis and even hepatocellular carcinoma. Salvianic acid A (SAA) is a phenolic acid component of Danshen, a Chinese herbal medicine with possible hepatoprotective properties. The purpose of this study was to investigate the effect of SAA on chronic alcoholic liver injury and its molecular mechanism. We found that SAA significantly inhibited alcohol-induced liver injury and ameliorated ethanol-induced...
Source: J Cell Mol Med - June 29, 2020 Category: Molecular Biology Authors: Lan Y, Yan R, Shan W, Chu J, Sun R, Wang R, Zhao Y, Wang Z, Zhang N, Yao J Tags: J Cell Mol Med Source Type: research

Homocysteine concentration and adenosine A2A receptor production by peripheral blood mononuclear cells in coronary artery disease patients.
Abstract Hyperhomocysteinemia is associated with coronary artery disease (CAD). The mechanistic aspects of this relationship are unclear. In CAD patients, homocysteine (HCy) concentration correlates with plasma level of adenosine that controls the coronary circulation via the activation of adenosine A2A receptors (A2A R). We addressed in CAD patients the relationship between HCy and A2A R production, and in cellulo the effect of HCy on A2A R function. 46 patients with CAD and 20 control healthy subjects were included. We evaluated A2A R production by peripheral blood mononuclear cells using Western blotting. We st...
Source: J Cell Mol Med - June 29, 2020 Category: Molecular Biology Authors: Deharo P, Marlinge M, Guiol C, Vairo D, Fromonot J, Mace P, Chefrour M, Gastaldi M, Bruzzese L, Gaubert M, Gaudry M, Kipson N, Criado C, Cuisset T, Paganelli F, Ruf J, Guieu R, Fenouillet E, Mottola G Tags: J Cell Mol Med Source Type: research

The ACE2 expression in Sertoli cells and germ cells may cause male reproductive disorder after SARS-CoV-2 infection.
Abstract The serious coronavirus disease-2019 (COVID-19) was first reported in December 2019 in Wuhan, China. COVID-19 is an infectious disease caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2). Angiotensin converting enzyme 2(ACE2) is the cellular receptor for SARS-CoV-2. Considering the critical roles of testicular cells for the transmission of genetic information between generations, we analyzed single-cell RNA-sequencing (scRNA-seq) data of adult human testis. The mRNA expression of ACE2 was expressed in both germ cells and somatic cells. Moreover, the positive rate of ACE2 in testes of in...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Shen Q, Xiao X, Aierken A, Yue W, Wu X, Liao M, Hua J Tags: J Cell Mol Med Source Type: research

A SNP of miR-146a is involved in bladder cancer relapse by affecting the function of bladder cancer stem cells via the miR-146a signallings.
In conclusion, two signalling pathways, miR-146a/YAP1/ALDH1A1 and miR-146a/COX2/PGE2/let7/SOX2, were modulated by miR-146a. As an SNP regulating the expression of miR-146a, the rs2910164 G > C SNP could be utilized as a biomarker for BC relapse. PMID: 32596945 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Wang T, Yang Y, Wang Z, Zhang X, Li D, Wei J Tags: J Cell Mol Med Source Type: research

FDPS promotes glioma growth and macrophage recruitment by regulating CCL20 via Wnt/ β-catenin signalling pathway.
In conclusion, overexpressed FDPS exhibits an immunomodulatory role in glioma. Therefore, targeting FDPS may be an effective therapeutic strategy for glioma. PMID: 32596949 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Chen Z, Chen G, Zhao H Tags: J Cell Mol Med Source Type: research

HDAC1-mediated deacetylation of LSD1 regulates vascular calcification by promoting autophagy in chronic renal failure.
Abstract Chronic renal failure (CRF) is commonly associated with various adverse consequences including pathological vascular calcification (VC), which represents a significant clinical concern. Existing literature has suggested the involvement of histone deacetylases (HDACs) in the progression of CRF-induced VC. However, the underlying molecular mechanisms associated with HDACs remain largely unknown. Therefore, we established the adenine-induced CRF rat model and in vitro VC models based on vascular smooth muscle cells (VSMCs) to examine HDAC1/lysine demethylase 1A (LSD1)/SESN2 as a novel molecular pathway in CR...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Zhou J, Zhou H, Liu C, Huang L, Lu D, Gao C Tags: J Cell Mol Med Source Type: research

The oncogenic role of MUC12 in RCC progression depends on c-Jun/TGF- β signalling.
The oncogenic role of MUC12 in RCC progression depends on c-Jun/TGF-β signalling. J Cell Mol Med. 2020 Jun 28;: Authors: Gao SL, Yin R, Zhang LF, Wang SM, Chen JS, Wu XY, Yue C, Zuo L, Tang M Abstract Renal cell carcinoma (RCC) is a common kidney cancer worldwide. Even though current treatments show promising therapeutic effectiveness, metastatic RCC still has limited therapeutic options so that novel treatments were urgently needed. Here, we identified that MUC12 was overexpressed in RCC patients and served as poor prognostic factor for RCC progression. Overexpression of MUC12 increased RCC cell...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Gao SL, Yin R, Zhang LF, Wang SM, Chen JS, Wu XY, Yue C, Zuo L, Tang M Tags: J Cell Mol Med Source Type: research

Hypoxia-induced miR-3677-3p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells by suppressing SIRT5.
In conclusion, the overexpression of miR-3677-3p mediated SIRT5 inhibition, which could increase proliferation, migration and invasion of HCC in hypoxic microenvironments. PMID: 32596968 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Yao B, Li Y, Niu Y, Wang L, Chen T, Guo C, Liu Q Tags: J Cell Mol Med Source Type: research

Novel evidence for oncogenic piRNA-823 as a promising prognostic biomarker and a potential therapeutic target in colorectal cancer.
In this study, we set up the cancer-promoting function recovery experiment of piRNA-823 by silencing G6PD gene to confirm the dominance of the above-mentioned pathways. Using clinical samples, we found that overexpression of piRNA-823 correlated with poor overall survival and predicted a poor response to adjuvant chemotherapy of patients with CRC. In a word, our research has further enriched the theory of piRNA-823 promoting the progression of CRC, and laid a solid foundation for the development of piRNA-823-based gene therapy for CRC and its use as a promising prognostic biomarker in CRC patients. PMID: 32596991 [Pub...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Feng J, Yang M, Wei Q, Song F, Zhang Y, Wang X, Liu B, Li J Tags: J Cell Mol Med Source Type: research

LncRNA SNHG3 promotes bladder cancer proliferation and metastasis through miR-515-5p/GINS2 axis.
Abstract Growing evidence suggests that long non-coding RNAs (lncRNAs) are associated with carcinogenesis. LncRNA small nucleolar RNA host gene 3 (SNHG3) is up-regulated in various cancers and positively associated with poor prognosis of these cancers. However, the precise role of lncRNA SNHG3 in bladder cancer (Bca) remains unclear. In our research, we first reported that lncRNA SNHG3 was up-regulated in bladder cancer tissues and positively related to poor clinical prognosis. Moreover, knockdown of lncRNA SNHG3 significantly suppressed the proliferation, migration, invasion and EMT process of Bca cells in vitro ...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Dai G, Huang C, Yang J, Jin L, Fu K, Yuan F, Zhu J, Xue B Tags: J Cell Mol Med Source Type: research

IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction.
Abstract Vascular endothelial growth factor (VEGF) is a well-known angiogenic factor, however its ability in promoting therapeutic angiogenesis following myocardial infarction (MI) is limited. Here, we aimed to investigate whether dual treatment with insulin-like growth factor binding protein-4 (IGFBP-4), an agent that protects against early oxidative damage, can be effective in enhancing the therapeutic effect of VEGF following MI. Combined treatment with IGFBP-4 enhanced VEGF-induced angiogenesis and prevented cell damage via enhancing the expression of a key angiogenic factor angiopoietin-1. Dual treatment with...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Wo D, Chen J, Li Q, Ma E, Yan H, Peng J, Zhu W, Fang Y, Ren DN Tags: J Cell Mol Med Source Type: research

Circ-AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR-296-3p/E-cadherin signals.
In conclusion, circ-AKT3 inhibited the extracellular matrix accumulation in diabetic nephropathy mesangial cells through modulating miR-296-3p/E-cadherin signals, which might offer novel potential opportunities for clinical diagnosis targets and therapeutic biomarkers for diabetic nephropathy. PMID: 32597022 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Tang B, Li W, Ji TT, Li XY, Qu X, Feng L, Bai S Tags: J Cell Mol Med Source Type: research

LncRNA TUG1 regulates ApoM to promote atherosclerosis progression through miR-92a/FXR1 axis.
This study aims to explore the possible mechanism of TUG1 regulating ApoM in AS. To this end, expression levels of TUG1 and ApoM were measured in high fat dieted C57BL/6J mice, normal dieted C57BL/6J mice, ob/ob mice and db/db mice. LV-TUG1 or sh-TUG1 was injected into C57BL/6J mice before isolating peritoneal macrophages to measure cholesterol efflux (CE) and expression levels of ABCA1, ABCG1 and SR-BI. Meanwhile, CE in RAW264.7 cells was also measured after cell transfection. Dual luciferase reporter assay and anti-AGO2 RIP were applied to verify the relationship among TUG1, FXR1 and miR-92a. Total cholesterol (TC), trig...
Source: J Cell Mol Med - June 28, 2020 Category: Molecular Biology Authors: Yang L, Li T Tags: J Cell Mol Med Source Type: research

Involvement of a novel circularRNA, hsa_circ_0000520, attenuates tumorigenesis of cervical cancer cell through competitively binding with miR-146b-3p.
In conclusion, circ_0000520 repressed CC progression in vitro and in vivo by sponging miR-146b-3p to release PAX5. PMID: 32592222 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 27, 2020 Category: Molecular Biology Authors: Zhang J, Cai R, Zhang Y, Wang X Tags: J Cell Mol Med Source Type: research

Reducing YAP expression in Pkd1 mutant mice does not improve the cystic phenotype.
Abstract The Hippo pathway is a highly conserved signalling route involved in organ size regulation. The final effectors of this pathway are two transcriptional coactivators, yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (WWTR1 or TAZ). Previously, we showed aberrant activation of the Hippo pathway in autosomal-dominant polycystic kidney disease (ADPKD), suggesting that YAP/TAZ might play a role in disease progression. Using antisense oligonucleotides (ASOs) in a mouse model for ADPKD, we efficiently down-regulated Yap levels in the kidneys. However, we did not see any effect ...
Source: J Cell Mol Med - June 27, 2020 Category: Molecular Biology Authors: Formica C, Kunnen S, Dauwerse JG, Mullick AE, Dijkstra KL, Scharpfenecker M, Peters DJM, DIPAK Consortium Tags: J Cell Mol Med Source Type: research

Dopamine and cAMP-regulated phosphoprotein 32kDa (DARPP-32), protein phosphatase-1 and cyclin-dependent kinase 5 expression in ovarian cancer.
Abstract Dopamine and cyclic-AMP activated phosphoprotein Mr32kDa (DARPP-32) is a central signalling protein in neurotransmission. Following DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 becomes a potent protein phosphatase 1 (PP1) inhibitor. DARPP-32 can itself inhibit PKA following DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). Increasing evidence indicates a role for DARPP-32 and its associated signalling pathways in cancer; however, its role in ovarian cancer remains unclear. Using immunohistochemistry, expression of DARPP-32, PP1 and Cdk5 was determined in a large cohort of prima...
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Martin SG, Zhang S, Yang S, Saidy B, Deen S, Storr SJ Tags: J Cell Mol Med Source Type: research

Growth differentiation factor 11 promotes differentiation of MSCs into endothelial-like cells for angiogenesis.
Abstract Growth differentiation factor 11 (GDF11) is a member of the transforming growth factor-β super family. It has multiple effects on development, physiology and diseases. However, the role of GDF11 in the development of mesenchymal stem cells (MSCs) is not clear. To explore the effects of GDF11 on the differentiation and pro-angiogenic activities of MSCs, mouse bone marrow-derived MSCs were engineered to overexpress GDF11 (MSCGDF11 ) and their capacity for differentiation and paracrine actions were examined both in vitro and in vivo. Expression of endothelial markers CD31 and VEGFR2 at the levels o...
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Zhang C, Lin Y, Liu Q, He J, Xiang P, Wang D, Hu X, Chen J, Zhu W, Yu H Tags: J Cell Mol Med Source Type: research

TIPE1-mediated autophagy suppression promotes nasopharyngeal carcinoma cell proliferation via the AMPK/mTOR signalling pathway.
Abstract Recent studies have shown that tumour necrosis factor-α-induced protein 8 like-1(TIPE1) plays distinct roles in different cancers. TIPE1 inhibits tumour proliferation and metastasis in a variety of tumours but acts as an oncogene in cervical cancer. The role of TIPE1 in nasopharyngeal carcinoma (NPC) remains unknown. Interestingly, TIPE1 expression was remarkably increased in NPC tissue samples compared to adjacent normal nasopharyngeal epithelial tissue samples in our study. TIPE1 expression was positively correlated with that of the proliferation marker Ki67 and negatively correlated with patient ...
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Liu Y, Qi X, Zhao Z, Song D, Wang L, Zhai Q, Zhang X, Zhao P, Xiang X Tags: J Cell Mol Med Source Type: research

Targeting IL-11 in the treatment of BK virus-associated haemorrhagic cystitis-A promising new approach.
CA Abstract The BK polyomavirus (BKPyV) has pathogenic relevance especially in immunocompromised patients. No causal therapy has been established yet. Therefore, new therapeutic targets need to be identified in experimental studies. A 3D organotypic cell culture model with primary urothelial cells and fibroblasts was used as infection model. The detection of virus replication was performed with quantitative polymerase chain reaction (qPCR), and immunohistochemistry (IHC) was also used for analysis. Interleukin levels were measured by enzyme-linked immunosorbent assay (ELISA). Interestingly, the signal transducer ...
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Schneidewind L, Neumann T, Krüger W, Hakenberg OW, Schmidt CA Tags: J Cell Mol Med Source Type: research

Exosomal MiR-500a-3p promotes cisplatin resistance and stemness via negatively regulating FBXW7 in gastric cancer.
In this study, we found that microRNA-500a-3p was highly expressed in cisplatin (DDP) resistant GC cells (MGC803/DDP and MKN45/DDP) and their secreted exosomes than that in the corresponding parental cells. MGC803/DDP-derived exosomes enhance DDP resistance and stemness properties of MGC803 recipient cells via exosomal delivery of miR-500a-3p in vitro and in vivo through targeting FBXW7. However, reintroduction of FBXW7 in MGC803 cells reverses miR-500a-3p-mediated DDP resistance as well as stemness properties. Furthermore, elevated miR-500a-3p in the plasma exosomes of GC patients is correlated with DDP resistance and the...
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Lin H, Zhang L, Zhang C, Liu P Tags: J Cell Mol Med Source Type: research

Antibacterial potential of Forsythia suspensa polysaccharide against resistant Enterobacter cloacae with SHV-12 extended-spectrum β-lactamase (ESBL).
In conclusion, these data showed that FSP exhibited potent good antibacterial activity against E cloacae producing SHV-12 ESBL via inhibition of SHV-12 ESBL gene expression, which may promote the development of novel natural antibacterial agents to treat infections caused by this drug-resistant bacterial pathogen. PMID: 32588543 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 25, 2020 Category: Molecular Biology Authors: Liu J, Lin L, Jia Z, Chen J, Zhao Z, Zhao Y, Xu Z, Guo Z Tags: J Cell Mol Med Source Type: research