CircSAMD4A aggravates H/R-induced cardiomyocyte apoptosis and inflammatory response by sponging miR-138-5p.
In conclusion, CircSAMD4A can sponge miR-138-5p to promote H/R-induced apoptosis and inflammatory response. PMID: 33219594 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 21, 2020 Category: Molecular Biology Authors: Hu X, Ma R, Cao J, Du X, Cai X, Fan Y Tags: J Cell Mol Med Source Type: research

Piperine protects against pyroptosis in myocardial ischaemia/reperfusion injury by regulating the miR-383/RP105/AKT signalling pathway.
This study focuses on whether PIP protects MIRI from pyroptosis via miR-383-dependent pathway. Rat MIRI model was established by 30 minutes of LAD ligation and 4 hours of reperfusion. Myocardial enzymes, histomorphology, structure and function were detected to evaluate MIRI. Recombinant adenoviral vectors for miR-383 overexpression or miR-383 silencing or RP105 knockdown were constructed, respectively. Luciferase reporter analysis was used to confirm RP105 as a target of miR-383. Pyroptosis-related markers were measured by Western blotting assay. The results showed that I/R provoked myocardial injury, as shown by...
Source: J Cell Mol Med - November 21, 2020 Category: Molecular Biology Authors: Guo X, Hu S, Liu JJ, Huang L, Zhong P, Fan ZX, Ye P, Chen MH Tags: J Cell Mol Med Source Type: research

Hypoxic bone marrow mesenchymal cell-extracellular vesicles containing miR-328-3p promote lung cancer progression via the NF2-mediated Hippo axis.
Abstract Lung cancer is the most aggressive tumour afflicting patients on a global scale. Extracellular vesicle (EV)-delivered microRNAs (miRs) have been reported to play critical roles in cancer development. The current study aimed to investigate the role of hypoxic bone marrow mesenchymal cell (BMSC)-derived EVs containing miR-328-3p in lung cancer. miR-328-3p expression was determined in a set of lung cancer tissues by RT-qPCR. BMSCs were infected with lentivirus-mediated miR-328-3p knock-down and then cultured in normoxic or hypoxic conditions, followed by isolation of EVs. Following ectopic expression and dep...
Source: J Cell Mol Med - November 21, 2020 Category: Molecular Biology Authors: Liu X, Jiang F, Wang Z, Tang L, Zou B, Xu P, Yu T Tags: J Cell Mol Med Source Type: research

Cardiac fibroblast miR-27a may function as an endogenous anti-fibrotic by negatively regulating Early Growth Response Protein 3 (EGR3).
Abstract Pathological myocardial fibrosis and hypertrophy occur due to chronic cardiac stress. The microRNA-27a (miR-27a) regulates collagen production across diverse cell types and organs to inhibit fibrosis and could constitute an important therapeutic avenue. However, its impact on hypertrophy and cardiac remodelling is less well-known. We employed a transverse aortic constriction (TAC) murine model of left ventricular pressure overload to investigate the in vivo effects of genetic miR-27a knockout, antisense inhibition of miR-27a-5p and fibroblast-specific miR-27a knockdown or overexpression. In silico Venn an...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Teng L, Huang Y, Guo J, Li B, Lin J, Ma L, Wang Y, Ye C, Chen Q Tags: J Cell Mol Med Source Type: research

Comprehensive analysis of partial epithelial mesenchymal transition-related genes in hepatocellular carcinoma.
This study implied that key p-EMT-related genes P4HA2, ITGA5, MMP9, MT1X, SPP1 could be prognostic biomarkers and potential targets of therapy for HCC patients. We constructed an mRNA-miRNA-lncRNA subnetwork containing p-EMT-related genes successfully, among which each component might be utilized as a prognostic biomarker of HCC. PMID: 33215860 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Lei Y, Yan W, Lin Z, Liu J, Tian D, Han P Tags: J Cell Mol Med Source Type: research

Herbal formula LLKL ameliorates hyperglycaemia, modulates the gut microbiota and regulates the gut-liver axis in Zucker diabetic fatty rats.
This study evaluated the LLKL anti-diabetic efficacy and its effect on gut microbiota to elucidate its mechanism of action in Zucker diabetic fatty rats. We found that administration of different LLKL concentrations (4.68, 2.34 and 1.17 g/kg/d) improved several diabetic parameters after a 6-week treatment. Moreover, LLKL modulated gut microbiota dysbiosis, increased the expression of occluding and maintained intestinal epithelial homeostasis, leading to a reduction in LPS, TNF-α and IL-6 levels. Hepatic transcriptomic analysis showed that the Toll-like receptor signalling pathway was markedly enriched by LLKL tr...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Li M, Ding L, Hu YL, Qin LL, Wu Y, Liu W, Wu LL, Liu TH Tags: J Cell Mol Med Source Type: research

Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction.
Abstract Chronic intermittent hypoxia (CIH) is the primary feature of obstructive sleep apnoea (OSA), a crucial risk factor for cardiovascular diseases. Long non-coding RNAs (lncRNAs) in myocardial infarction (MI) pathogenesis have drawn considerable attention. However, whether CIH participates in the modulation of lncRNA profiles during MI is yet unclear. To investigate the influence of CIH on MI, cardiac damage was assessed by histology and echocardiography, and lncRNA and mRNA integrated microarrays were screened. MI mouse model showed myocardial hypertrophy, aggravated inflammation and fibrosis, and compromise...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Hu C, Li J, Du Y, Li J, Yang Y, Jia Y, Peng L, Qin Y, Wei Y Tags: J Cell Mol Med Source Type: research

Procyanidin B2 improves endothelial progenitor cell function and promotes wound healing in diabetic mice via activating Nrf2.
Abstract One of the major reasons for the delayed wound healing in diabetes is the dysfunction of endothelial progenitor cells (EPCs) induced by hyperglycaemia. Improvement of EPC function may be a potential strategy for accelerating wound healing in diabetes. Procyanidin B2 (PCB2) is one of the major components of procyanidins, which exhibits a variety of potent pharmacological activities. However, the effects of PCB2 on EPC function and diabetic wound repair remain elusive. We evaluated the protective effects of PCB2 in EPCs with high glucose (HG) treatment and in a diabetic wound healing model. EPCs derive...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Fan J, Liu H, Wang J, Zeng J, Tan Y, Wang Y, Yu X, Li W, Wang P, Yang Z, Dai X Tags: J Cell Mol Med Source Type: research

A novel autophagy-related lncRNA prognostic risk model for breast cancer.
In this study, we identified autophagy-related lncRNAs in breast cancer by constructing a co-expression network of autophagy-related mRNAs-lncRNAs from The Cancer Genome Atlas (TCGA). We evaluated the prognostic value of these autophagy-related lncRNAs by univariate and multivariate Cox proportional hazards analyses and eventually obtained a prognostic risk model consisting of 11 autophagy-related lncRNAs (U62317.4, LINC01016, LINC02166, C6orf99, LINC00992, BAIAP2-DT, AC245297.3, AC090912.1, Z68871.1, LINC00578 and LINC01871). The risk model was further validated as a novel independent prognostic factor for breast cancer p...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Li X, Jin F, Li Y Tags: J Cell Mol Med Source Type: research

Nucleolin regulates the proliferation of vascular smooth muscle cells in atherosclerotic via Aurora B.
In this study, we aimed to investigate the role of nucleolin in VSMCs proliferation and cell cycle. The expression of nucleolin increased in VSMCs of mice with aortas advanced plaques. With the left common carotid-artery ligation-injury model, immunofluorescence staining revealed that nucleolin and Ki67 expression increased in VSMCs in mice left carotid artery compared with right carotid artery after surgery. POVPC or ox-LDL up-regulated nucleolin mRNA and protein expression in a dose- and time-dependent manner in HAVSMCs. POVPC (5μg/ml) or ox-LDL (50μg/ml) promoted the proliferation of HAVSMCs. Nucleolin ablation re...
Source: J Cell Mol Med - November 20, 2020 Category: Molecular Biology Authors: Sun H, Huang L, Liang P, Tang Y, Chen C, Chen H, Lin X, Luo Z, Li Y, Jiang B, Xiao X Tags: J Cell Mol Med Source Type: research

The immuno-reactivity of polypseudorotaxane functionalized magnetic CDMNP-PEG-CD nanoparticles.
Abstract pH-magnetic dual-responsive nanocomposites have been widely used in drug delivery and gene therapy. Recently, a polypseudorotaxane functionalized magnetic nanoparticle (MNP) was developed by synthesizing the magnetic nanoparticles with cyclodextrin (CD) molecules (CDMNP) via polyethylene glycol (PEG) (CDMNP-PEG-CD). The purpose of this study was to explore the antigenicity and immunogenicity of the nanoparticles in vivo prior to their further application explorations. Here, nanoparticles were assessed in vivo for retention, bio-distribution and immuno-reactivity. The results showed that, once administered...
Source: J Cell Mol Med - November 19, 2020 Category: Molecular Biology Authors: Lan H, Huang T, Xiao J, Liao Z, Ouyang J, Dong J, Xian CJ, Hu J, Wang L, Ke Y, Liao H Tags: J Cell Mol Med Source Type: research

Maltol prevents the progression of osteoarthritis by targeting PI3K/Akt/NF- κB pathway: In vitro and in vivo studies.
Maltol prevents the progression of osteoarthritis by targeting PI3K/Akt/NF-κB pathway: In vitro and in vivo studies. J Cell Mol Med. 2020 Nov 19;: Authors: Lu H, Fu C, Kong S, Wang X, Sun L, Lin Z, Luo P, Jin H Abstract Osteoarthritis (OA), a prevalent degenerative arthritis disease, principle characterized by the destruction of cartilage and associated with the inflammatory response. Maltol, a product formed during the processing of red ginseng (Panax ginseng, CA Meyer), has been reported to have the potential effect of anti-inflammatory. However, its specific mechanisms are not demonstrated. W...
Source: J Cell Mol Med - November 19, 2020 Category: Molecular Biology Authors: Lu H, Fu C, Kong S, Wang X, Sun L, Lin Z, Luo P, Jin H Tags: J Cell Mol Med Source Type: research

Multiple potential targets of opioids in the treatment of acute respiratory distress syndrome from COVID-19.
Abstract COVID-19 can present with a variety of clinical features, ranging from asymptomatic or mild respiratory symptoms to fulminant acute respiratory distress syndrome (ARDS) depending on the host's immune responses and the extent of the associated pathologies. This implies that several measures need to be taken to limit severely impairing symptoms caused by viral-induced pathology in vital organs. Opioids are most exploited for their analgesic effects but their usage in the palliation of dyspnoea, immunomodulation and lysosomotropism may represent potential usages of opioids in COVID-19. Here, we describe the ...
Source: J Cell Mol Med - November 19, 2020 Category: Molecular Biology Authors: Cismaru CA, Cismaru GL, Nabavi SF, Ghanei M, Burz CC, Nabavi SM, Berindan Neagoe I Tags: J Cell Mol Med Source Type: research

Sophoridine suppresses lenvatinib-resistant hepatocellular carcinoma growth by inhibiting RAS/MEK/ERK axis via decreasing VEGFR2 expression.
This study aims to investigate the underlying mechanism of lenvatinib resistance and explore the potential drug to improve the treatment for lenvatinib-resistant (LR) HCC. Here, we developed two human LR HCC cell lines by culturing with long-term exposure to lenvatinib. Results showed that the vascular endothelial growth factor receptors (VEGFR)2 expression and its downstream RAS/MEK/ERK signalling were obviously up-regulated in LR HCC cells, whereas the expression of VEGFR1, VEGFR3, FGFR1-4 and PDGFRα/β showed no difference. Furthermore, ETS-1 was identified to be responsible for VEGFR2 mediated lenvatinib resi...
Source: J Cell Mol Med - November 18, 2020 Category: Molecular Biology Authors: Zhao Z, Zhang D, Wu F, Tu J, Song J, Xu M, Ji J Tags: J Cell Mol Med Source Type: research

Circular RNA circ_0008305 aggravates hepatocellular carcinoma growth through binding to miR-186 and inducing TMED2.
Abstract Dysregulation of circRNAs is reported to exert crucial roles in cancers, including hepatocellular carcinoma (HCC). So far, the function of circRNAs in HCC development remains poorly known. Currently, our data showed that circ_0008305 was highly elevated in HCC cell lines and 30 paired tissue samples of HCC. As evidenced, suppression of circ_0008305 repressed HCC cell growth significantly. Meanwhile, up-regulation of circ_0008305 significantly reduced HCC cell growth. Mechanistically, we displayed that circ_0008305 could bind with miR-186 by using bioinformatics analysis. miR-186 has been reported to be a ...
Source: J Cell Mol Med - November 18, 2020 Category: Molecular Biology Authors: Zhang X, Hao HH, Zhuang HW, Wang J, Sheng Y, Xu F, Dou J, Chen C, Shen Y Tags: J Cell Mol Med Source Type: research

MiR155 modulates vascular calcification by regulating Akt-FOXO3a signalling and apoptosis in vascular smooth muscle cells.
In conclusion, miR155 deficiency attenuates vascular calcification by increasing Akt phosphorylation and FOXO3a degradation, and thus reducing VSMC apoptosis induced by calcification medium. PMID: 33210462 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 18, 2020 Category: Molecular Biology Authors: Li Y, Sun W, Saaoud F, Wang Y, Wang Q, Hodge J, Hui Y, Yin S, Lessner SM, Kong X, Fan D Tags: J Cell Mol Med Source Type: research

Chromosomal mosaicism detected by karyotyping and chromosomal microarray analysis in prenatal diagnosis.
Abstract To investigate the incidence and clinical significance of chromosomal mosaicism (CM) in prenatal diagnosis by G-banding karyotyping and chromosomal microarray analysis (CMA). This is a single-centre retrospective study of invasive prenatal diagnosis for CM. From 5758 karyotyping results and 6066 CMA results, 104 foetal cases with CM were selected and analysed further. In total, 50% (52/104) of foetal cases with CM were affected by ultrasound-detectable phenotypes. Regardless of whether they were singleton or twin pregnancies, isolated structural defects in one system (51.35%, 19/37 in singletons; 86.67%, ...
Source: J Cell Mol Med - November 17, 2020 Category: Molecular Biology Authors: Zhang Y, Zhong M, Zheng D Tags: J Cell Mol Med Source Type: research

Mixed-lineage leukaemia 1 contributes to endometrial stromal cells progesterone responsiveness during decidualization.
Abstract Studies have reported that non-receptive endometrium or abnormal decidualization was closely related to recurrent implantation failure (RIF). MLL1 is a histone H3 lysine 4 trimethylation (H3K4me3) transferase that regulates the transcriptional activation of target genes. The role of MLL1 has been underexplored during decidualization. In our research, we found the expression of MLL1 was closely related to endometrial receptivity, and it was responsible to hormone stimulation. Inhibiting the function of MLL1 by MM102 reduced the transformation of HESCs. Furthermore, down-regulation of MLL1 by siRNA transfec...
Source: J Cell Mol Med - November 17, 2020 Category: Molecular Biology Authors: Xiong Y, Wang Y, Ma L, Zhang Y, Qu X, Huang L, Wen X, Liu H, Zhang M, Zhang Y Tags: J Cell Mol Med Source Type: research

Small molecules restore the function of mutant CLC5 associated with Dent disease.
Abstract Dent disease type 1 is caused by mutations in the CLCN5 gene that encodes CLC5, a 2Cl- /H+ exchanger. The CLC5 mutants that have been functionally analysed constitute three major classes based on protein expression, cellular localization and channel function. We tested two small molecules, 4-phenylbutyrate (4PBA) and its analogue 2-naphthoxyacetic acid (2-NOAA), for their effect on mutant CLC5 function and expression by whole-cell patch-clamp and Western blot, respectively. The expression and function of non-Class I CLC5 mutants that have reduced function could be restored by either treatment. Cell viabil...
Source: J Cell Mol Med - November 16, 2020 Category: Molecular Biology Authors: Liu J, Sadeh TT, Lippiat JD, Thakker RV, Black GC, Manson F Tags: J Cell Mol Med Source Type: research

circ-AKT3 aggravates renal ischaemia-reperfusion injury via regulating miR-144-5p /Wnt/ β-catenin pathway and oxidative stress.
In conclusion, our study suggests that the circAKT3 is involved RI/R injury progression through regulating miR-144-5p/Wnt/β-catenin pathway and oxidative stress. PMID: 33200535 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 16, 2020 Category: Molecular Biology Authors: Xu Y, Jiang W, Zhong L, Li H, Bai L, Chen X, Lin Y, Zheng D Tags: J Cell Mol Med Source Type: research

HN1L promotes migration and invasion of breast cancer by up-regulating the expression of HMGB1.
Abstract Recent reports showed that haematological and neurological expressed 1-like (HN1L) gene participated in tumorigenesis and tumour invasion. However, the expression and role of HN1L in breast cancer remain to be investigated. Here, bioinformatics, western blot and immunohistochemistry were used to detect the expression of HN1L in breast cancer. Wound healing, transwell assay, immunofluorescence assay and mass spectrum were used to explore the role and mechanism of HN1L on the migration and invasion of breast cancer, which was confirmed in vivo using a nude mice model. Results showed that HN1L was significan...
Source: J Cell Mol Med - November 16, 2020 Category: Molecular Biology Authors: Jiao D, Zhang J, Chen P, Guo X, Qiao J, Zhu J, Wang L, Lu Z, Liu Z Tags: J Cell Mol Med Source Type: research

CX3 CL1 promotes tumour cell by inducing tyrosine phosphorylation of cortactin in lung cancer.
Abstract It has been reported that chemokine CX3 CL1 can regulate various tumours by binding to its unique receptor CX3 CR1. However, the effect of CX3 CL1-CX3 CR1 on the lung adenocarcinoma and lung squamous cell carcinoma is still unclear. Here, we showed that CX3 CL1 can further invasion and migration of lung adenocarcinoma A549 and lung squamous cell carcinoma H520. In addition, Western blot and immunofluorescence test indicated CX3 CL1 up-regulated the phosphorylation level of cortactin, which is a marker of cell pseudopodium. Meanwhile, the phosphorylation levels of c-Src and c-Abl, which are closely related...
Source: J Cell Mol Med - November 15, 2020 Category: Molecular Biology Authors: Fan M, Wu J, Li X, Jiang Y, Wang X, Bie M, Weng Y, Chen S, Chen B, An L, Zhang M, Huang G, Zhu M, Shi Q Tags: J Cell Mol Med Source Type: research

Cask methylation involved in the injury of insulin secretion function caused by interleukin1- β.
This study aims to unveil the function of CASK in IL-1β-induced insulin secretion dysfunction and the possible mechanism thereof. Islets of Sprague-Dawley (SD) rats and INS-1 cells stimulated with IL-1β were utilized as models of chronic inflammation. Insulin secretion function associated with Cask and DNA methyltransferases (DNMT) expression were assessed. The possible mechanisms of IL-1β-induced pancreatic β-cell dysfunction were also explored. In this study, CASK overexpression effectively improved IL-1β-induced islet β-cells dysfunction, increased insulin secretion. DNA methyltransferases ...
Source: J Cell Mol Med - November 14, 2020 Category: Molecular Biology Authors: Wang TY, Liu XJ, Xie JY, Yuan QZ, Wang Y Tags: J Cell Mol Med Source Type: research

Circ_0075829 facilitates the progression of pancreatic carcinoma by sponging miR-1287-5p and activating LAMTOR3 signalling.
Abstract Pancreatic cancer (PC) is a leading cause of cancer-related mortality globally. Though increasing evidence has demonstrated that circular RNAs (circRNAs) are linked to the development and progression of cancers, the biological functions of circRNAs in PC remain largely unexplored so far. Based on previous studies, Hsc_circ_0075829 (circ_0075829) was screened out and then further identified in PC clinical specimens and cell lines by real-time PCR. After the stability tests, a series of in vitro and in vivo functional experiments were performed to investigate the role of circ_0075829 in PC development. Furt...
Source: J Cell Mol Med - November 13, 2020 Category: Molecular Biology Authors: Zhang X, Xue C, Cui X, Zhou Z, Fu Y, Yin X, Wu S, Gong Y, Liu Y, Zhu C, Qin X Tags: J Cell Mol Med Source Type: research

Chronic shift-lag promotes NK cell ageing and impairs immunosurveillance in mice by decreasing the expression of CD122.
In conclusion, our findings suggest that chronic circadian disruption attenuates NK cell cytolytic activity by decreasing the expression of CD122. PMID: 33185980 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 13, 2020 Category: Molecular Biology Authors: Zeng X, Liang C, Yao J Tags: J Cell Mol Med Source Type: research

Characterizing heterogeneity of non-small cell lung tumour microenvironment to identify signature prognostic genes.
This study focused on the characterization of immune infiltration profiling in patients with NSCLC and its correlation with survival outcome. All TCGA samples were divided into three heterogeneous clusters based on immune cell profiles: cluster 1 ('low infiltration' cluster), cluster 2 ('heterogeneous infiltration' cluster) and cluster 3 ('high infiltration' cluster). The immune cells were responsible for a significantly favourable prognosis for the 'high infiltration' community. Cluster 1 had the lowest cytotoxic activity, tumour-infiltrating lymphocytes and interferon-gamma (IFN-γ), as well as immune checkpoint mol...
Source: J Cell Mol Med - November 12, 2020 Category: Molecular Biology Authors: Mi K, Chen F, Qian Z, Chen J, Lv D, Zhang C, Xu Y, Wang H, Zhang Y, Jiang Y, Shang D Tags: J Cell Mol Med Source Type: research

Low let-7d exosomes from pulmonary vascular endothelial cells drive lung pericyte fibrosis through the TGF βRI/FoxM1/Smad/β-catenin pathway.
Low let-7d exosomes from pulmonary vascular endothelial cells drive lung pericyte fibrosis through the TGFβRI/FoxM1/Smad/β-catenin pathway. J Cell Mol Med. 2020 Nov 12;: Authors: Xie H, Gao YM, Zhang YC, Jia MW, Peng F, Meng QH, Wang YC Abstract The pathogenesis of pulmonary fibrosis (PF) was mediated by the progressive deposition of excessive extracellular matrix, but little is known about the regulatory mechanisms of fibrogenesis by lung pericytes. The mouse PF model was established by treatment with bleomycin, followed by isolation of exosomes from mouse broncho-alveolar lavage fluids by ...
Source: J Cell Mol Med - November 12, 2020 Category: Molecular Biology Authors: Xie H, Gao YM, Zhang YC, Jia MW, Peng F, Meng QH, Wang YC Tags: J Cell Mol Med Source Type: research

CircFAT1 promotes hepatocellular carcinoma progression via miR-30a-5p/REEP3 pathway.
In conclusion, circFAT1 sponges miR-30a-5p to regulate the expression of REEP3, thus promoting hepatocarcinogenesis. New HCC diagnosis or treatment strategies may be developed from circFAT1 as a target. PMID: 33179443 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 11, 2020 Category: Molecular Biology Authors: Wei H, Yan S, Hui Y, Liu Y, Guo H, Li Q, Li J, Chang Z Tags: J Cell Mol Med Source Type: research

Cranial irradiation alters neuroinflammation and neural proliferation in the pituitary gland and induces late-onset hormone deficiency.
Abstract Cranial radiotherapy induces endocrine disorders and reproductive abnormalities, particularly in long-term female cancer survivors, and this might in part be caused by injury to the pituitary gland, but the underlying mechanisms are unknown. The aim of this study was to investigate the influence of cranial irradiation on the pituitary gland and related endocrine function. Female Wistar rat pups on postnatal day 11 were subjected to a single dose of 6 Gy whole-head irradiation, and hormone levels and organ structure in the reproductive system were examined at 20 weeks after irradiation. We found ...
Source: J Cell Mol Med - November 10, 2020 Category: Molecular Biology Authors: Xu Y, Sun Y, Zhou K, Xie C, Li T, Wang Y, Zhang Y, Rodriguez J, Zhang X, Shao R, Wang X, Zhu C Tags: J Cell Mol Med Source Type: research

HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.
Abstract Prostate cancer is the most common malignancy in men in developed countries. In previous study, we identified HNF1B (Hepatocyte Nuclear Factor 1β) as a downstream effector of Enhancer of zeste homolog 2 (EZH2). HNF1B suppresses EZH2-mediated migration of two prostate cancer cell lines via represses the EMT process by inhibiting SLUG expression. Besides, HNF1B expression inhibits cell proliferation through unknown mechanisms. Here, we demonstrated that HNF1B inhibited the proliferation rate of prostate cancer cells. Overexpression of HNF1B in prostate cancer cells led to the arrest of G1 cell cycle an...
Source: J Cell Mol Med - November 10, 2020 Category: Molecular Biology Authors: Lu W, Sun J, Zhou H, Wang F, Zhao C, Li K, Fan C, Ding G, Wang J Tags: J Cell Mol Med Source Type: research

Circ_0067835 sponges miR-324-5p to induce HMGA1 expression in endometrial carcinoma cells.
In conclusion, circ_0067835 can compete with miR-324-5p, resulting in HMGA1 up-regulation, and therefore induce the development of endometrial cancer. PMID: 33169939 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 10, 2020 Category: Molecular Biology Authors: Liu Y, Chang Y, Cai Y Tags: J Cell Mol Med Source Type: research

Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling.
Abstract AMP-activated protein kinase (AMPK) is an intracellular sensor of energy homoeostasis that is activated under energy stress and suppressed in energy surplus. AMPK activation leads to inhibition of anabolic processes that consume ATP. Osteogenic differentiation is a process that highly demands ATP during which AMPK is inhibited. The bone morphogenetic proteins (BMPs) signalling pathway plays an essential role in osteogenic differentiation. The present study examines the inhibitory effect of metformin on BMP signalling, osteogenic differentiation and trauma-induced heterotopic ossification. Our results show...
Source: J Cell Mol Med - November 10, 2020 Category: Molecular Biology Authors: Lin H, Shi F, Jiang S, Wang Y, Zou J, Ying Y, Huang D, Luo L, Yan X, Luo Z Tags: J Cell Mol Med Source Type: research

Circulating exosomal small RNAs are promising non-invasive diagnostic biomarkers for gastric cancer.
In conclusion, our findings indicate that serum exosomal piRNAs are promising non-invasive diagnostic biomarkers for GC patients and potential markers for monitoring metastasis. PMID: 33169519 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 9, 2020 Category: Molecular Biology Authors: Ge L, Zhang N, Li D, Wu Y, Wang H, Wang J Tags: J Cell Mol Med Source Type: research

Tumour necrosis factor- α promotes BMHSC differentiation by increasing P2X7 receptor in oestrogen-deficient osteoporosis.
Tumour necrosis factor-α promotes BMHSC differentiation by increasing P2X7 receptor in oestrogen-deficient osteoporosis. J Cell Mol Med. 2020 Nov 09;: Authors: Lu J, Zhou Z, Ma J, Lu N, Lei Z, Du D, Chen A Abstract The exact mechanism of tumour necrosis factor α (TNF-α) promoting osteoclast differentiation is not completely clear. A variety of P2 purine receptor subtypes have been confirmed to be widely involved in bone metabolism. Thus, the purpose of this study was to explore whether P2 receptor is involved in the differentiation of osteoclasts. Mouse bone marrow haematopoietic ste...
Source: J Cell Mol Med - November 9, 2020 Category: Molecular Biology Authors: Lu J, Zhou Z, Ma J, Lu N, Lei Z, Du D, Chen A Tags: J Cell Mol Med Source Type: research

PDE1 or PDE5 inhibition augments NO-dependent hypoxic constriction of porcine coronary artery via elevating inosine 3',5'-cyclic monophosphate level.
Abstract Hypoxic coronary vasospasm may lead to myocardial ischaemia and cardiac dysfunction. Inosine 3',5'-cyclic monophosphate (cIMP) is a putative second messenger to mediate this pathological process. Nevertheless, it remains unclear as to whether levels of cIMP can be regulated in living tissue such as coronary artery and if so, what is the consequence of this regulation on hypoxia-induced vasoconstriction. In the present study, we found that cIMP was a key determinant of hypoxia-induced constriction but not that of the subsequent relaxation response in porcine coronary arteries. Subsequently, coronary arteri...
Source: J Cell Mol Med - November 9, 2020 Category: Molecular Biology Authors: Nan Y, Zeng X, Jin Z, Li N, Chen Z, Chen J, Wang D, Wang Y, Lin Z, Ying L Tags: J Cell Mol Med Source Type: research

Combining bioinformatics techniques to explore the molecular mechanisms involved in pancreatic cancer metastasis and prognosis.
This article aims to explore the underlying molecular mechanisms and prognosis-related genes in pancreatic cancer metastasis. Pancreatic cancer metastasis-related gene chip data were downloaded from GENE EXPRESSION OMNIBUS(GEO)database. Differentially expressed genes were screened after R-package pre-treatment. Functional annotations and related signalling pathways were analysed using DAVID software. GEPIA (Gene Expression Profiling Interactive Analysis) was used to perform prognostic analysis, and differential genes associated with prognosis were screened and validated using data from GEO. We screened 40 he...
Source: J Cell Mol Med - November 9, 2020 Category: Molecular Biology Authors: Xu JS, Wang X, He J, Liao KL, Wang XZ Tags: J Cell Mol Med Source Type: research

Farnesoid X receptor activation induces antitumour activity in colorectal cancer by suppressing JAK2/STAT3 signalling via transactivation of SOCS3 gene.
In conclusion, our study demonstrates that targeting FXR and improving its function might be a promising strategy for CRC treatment. PMID: 33164339 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 9, 2020 Category: Molecular Biology Authors: Li S, Xu Z, Guo J, Zheng J, Sun X, Yu J Tags: J Cell Mol Med Source Type: research

Polarization of ADAM17-driven EGFR signalling in electric field-guided collective migration of epidermal sheets.
In conclusion, our study indicated that ADAM17 contributed to the collective directional movement of the epidermal monolayer by driving HB-EGF release and activating EGFR under EFs, and this pathway also mediated the polarization of F-actin in migrating sheets, which is essential in directional migration. PMID: 33164313 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 8, 2020 Category: Molecular Biology Authors: Jia N, Liu J, Zhu G, Liang Y, Wang Y, Wang W, Chen Y, Yang J, Zhang W, Zhang J Tags: J Cell Mol Med Source Type: research

Differential expression of CD64 in patients with Mycobacterium tuberculosis infection: A potential biomarker for clinical diagnosis and prognosis.
Abstract To evaluate the clinical utility of neutrophil (n)CD64 index to diagnose pulmonary tuberculosis (PTB) and extrapulmonary TB (ePTB) and to predict the outcome of Mycobacterium tuberculosis infection. We recruited 189 patients with active TB and 140 controls and measured the differential expression of nCD64 index using flow cytometry. The receiver operating characteristics (ROC) curve analysis was performed to estimate the diagnostic performance of the nCD64 index and T-SPOT.TB assay for the diagnosis of TB. Furthermore, we analysed whether the nCD64 index in patients with TB was correlated with inflammator...
Source: J Cell Mol Med - November 8, 2020 Category: Molecular Biology Authors: Liu Q, Gao Y, Ou Q, Xu Y, Zhou Z, Li T, Lu Y, Sun F, Zhou X, Li Y, Shao L, Zhang W Tags: J Cell Mol Med Source Type: research

Neurexin-2 is a potential regulator of inflammatory pain in the spinal dorsal horn of rats.
Abstract Chronic pain is one of the serious conditions that affect human health and remains cure still remains a serious challenge as the molecular mechanism remains largely unclear. Here, we used label-free proteomics to identify potential target proteins that regulate peripheral inflammatory pain and reveal its mechanism of action. Inflammation in peripheral tissue was induced by injecting complete Freund's adjuvant (CFA) into rat hind paw. A proteomic method was adopted to compare the spinal dorsal horn (SDH) in peripheral inflammatory pain (PIP) model rats with controls. Differential proteins were identified i...
Source: J Cell Mol Med - November 8, 2020 Category: Molecular Biology Authors: Xu L, Feng Q, Deng H, Zhang X, Ni H, Yao M Tags: J Cell Mol Med Source Type: research

Thidiazuron decreases epithelial-mesenchymal transition activity through the NF-kB and PI3K/AKT signalling pathways in breast cancer.
Abstract Breast cancer is the major type among the women population globally. The treatment of cancer metastasis has made modest progress due to multiple factors. Thidiazuron (TDZ) is a novel plant growth regulator that has been shown to have anticancer effects. Therefore, we explored the anti-metastatic potentials of TDZ in cell lines by assessing its potential to suppress the epithelial-mesenchymal transition (EMT). We pretreated the BEAS-2B and breast cancer (MDA-MB-231) cells with TDZ and deliberated alteration in a cell viability, mammosphere, migration, NF-кB signalling, PI3K/AKT signalling and matrix metal...
Source: J Cell Mol Med - November 7, 2020 Category: Molecular Biology Authors: Rajendran P, Ben Ammar R, Al-Saeedi FJ, Elsayed Mohamed M, Islam M, Al-Ramadan SY Tags: J Cell Mol Med Source Type: research

Asprin-loaded strontium-containing α-calcium sulphate hemihydrate/nano-hydroxyapatite composite promotes regeneration of critical bone defects.
This study attempted to incorporate aspirin into it to produce a better bone graft material for critical bone defects. After 5% Sr-α-CSH was prepared by coprecipitation and hydrothermal methods, it was mixed with aspirin solution of different concentrations (50 μg/ml, 200 μg/ml, 800 μg/ml and 3200 μg/ml) at a fixed liquid-solid ratio (0.54 v/w) to obtain aspirin-loaded Sr-α-CSH/n-HA composite. In vitro experiments were performed on the composite extracts. The tibial defects (3 mm*5 mm) in SD rat model were filled with the composite for 4 weeks and 12 weeks to ...
Source: J Cell Mol Med - November 7, 2020 Category: Molecular Biology Authors: Jiang Y, Qin H, Wan H, Yang J, Yu Q, Jiang M, Yu B Tags: J Cell Mol Med Source Type: research

Unique inflammatory signature in haemophilic arthropathy: miRNA changes due to interaction between blood and fibroblast-like synoviocytes.
Abstract In haemophilia, the recurrence of hemarthrosis leads to irreversible arthropathy termed haemophilic arthropathy (HA). However, HA is a unique form of arthropathy in which resident cells, such as fibroblast-like synoviocytes (FLS), come into direct contact with blood. Therefore, we hypothesized that FLS in HA could have a unique inflammatory signature as a consequence of their contact with blood. We demonstrated with ELISA and ELISPOT analyses that HA-FLS expressed a unique profile of cytokine secretion, which differed from that of non-HA-FLS, mainly consisting of cytokines involved in innate immunity. We ...
Source: J Cell Mol Med - November 7, 2020 Category: Molecular Biology Authors: Mignot S, Cagnard N, Albaud B, Bally C, Siavellis J, Hermine O, Frenzel L Tags: J Cell Mol Med Source Type: research

Identification of mini-chromosome maintenance 8 as a potential prognostic marker and its effects on proliferation and apoptosis in gastric cancer.
Abstract Mini-chromosome maintenance (MCM) proteins play important roles in initiating eukaryotic genome replication. The MCM family of proteins includes several members associated with the development and progression of certain cancers. We performed online data mining to assess the expression of MCMs in gastric cancer (GC) and the correlation between their expression and survival in patients with GC. Notably, MCM8 expression was undoubtedly up-regulated in GC, and higher expression correlated with shorter overall survival (OS) and progression-free survival (PFS) in patients with GC. However, the role of MCM8 in G...
Source: J Cell Mol Med - November 6, 2020 Category: Molecular Biology Authors: Huang B, Lin M, Lu L, Chen W, Tan J, Zhao J, Cao Z, Zhu X, Lin J Tags: J Cell Mol Med Source Type: research

gB co-immunization with GP96 enhances pulmonary-resident CD8 T cells and exerts a long-term defence against MCMV pneumonitis.
In this study, N-terminal domain of GP96 (GP96-NT) was used as a mucosal adjuvant to enhance the induction of pulmonary-resident CD8 T cells elicited by MCMV glycoprotein B (gB) vaccine. Mice were intranasally co-immunized with 50 μg pgB and equal amount of pGP96-NT vaccine 4 times at 2-week intervals, and then i.n. challenged with MCMV at 16 weeks after the last immunization. Compared with pgB immunization alone, co-immunization with pgB/pGP96-NT enhanced a long-lasting protection against MCMV pneumonitis by significantly improved pneumonitis pathology, enhanced bodyweight, reduced viral burdens and increased...
Source: J Cell Mol Med - November 6, 2020 Category: Molecular Biology Authors: Guo B, Xu P, Chai D, Cao L, Liu L, Song T, Hu S, Chen Y, Yan X, Xu T Tags: J Cell Mol Med Source Type: research

IFN- γ+ IL-17+ Th17 cells regulate fibrosis through secreting IL-21 in systemic scleroderma.
This study aimed to explore the function of IFN-γ+ IL-17+ Th17 cells on fibrosis in systemic scleroderma (SSc). Blood and skin samples were collected from 20 SSc cases and 10 healthy individuals. The percentage of IFN-γ+ IL-17+ Th17 cells was detected using flow cytometry. The in vitro induction of IFN-γ+ IL-17+ Th17 cells was performed adopting PHA and rIL-12. Gene expression was detected via quantitative real-time polymerase chain reaction (qRT-PCR), whereas western blot analysis was adopted for protein analysis. The distribution of IFN-γ+ IL-17+ Th17 cells was significantly increased in SSc cases...
Source: J Cell Mol Med - November 6, 2020 Category: Molecular Biology Authors: Xing X, Li A, Tan H, Zhou Y Tags: J Cell Mol Med Source Type: research

Mesenchymal stroma/stem-like cells of GARP knockdown inhibits cell proliferation and invasion of mouse colon cancer cells (MC38) through exosomes.
In conclusion, MSC-derived exosomes targeting GARP are a potential strategy for cancer therapy. PMID: 33155413 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 5, 2020 Category: Molecular Biology Authors: Xing H, Liang C, Xu X, Sun H, Ma X, Jiang Z Tags: J Cell Mol Med Source Type: research

Aldosterone enhances high phosphate-induced vascular calcification through inhibition of AMPK-mediated autophagy.
Abstract It remains unclear whether the necessity of calcified mellitus induced by high inorganic phosphate (Pi) is required and the roles of autophagy plays in aldosterone (Aldo)-enhanced vascular calcification (VC) and vascular smooth muscle cell (VSMC) osteogenic differentiation. In the present study, we found that Aldo enhanced VC both in vivo and in vitro only in the presence of high Pi, alongside with increased expression of VSMC osteogenic proteins (BMP2, Runx2 and OCN) and decreased expression of VSMC contractile proteins (α-SMA, SM22α and smoothelin). However, these effects were blocked by min...
Source: J Cell Mol Med - November 4, 2020 Category: Molecular Biology Authors: Gao JW, He WB, Xie CM, Gao M, Feng LY, Liu ZY, Wang JF, Huang H, Liu PM Tags: J Cell Mol Med Source Type: research

Ursodeoxycholic acid protects against lung injury induced by fat embolism syndrome.
Abstract Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a life-threatening disease with a high mortality rate, which was a common complication of fat embolism syndrome (FES). Ursodeoxycholic acid (UDCA) has been reported to exert potent anti-inflammatory effects under various conditions. In vivo, perinephric fat was injected via tail vein to establish a rat FES model, the anti-inflammatory effects of UDCA on FES-induced lung injury were investigated through histological examination, ELISA, qRT-PCR, Western blot and immunofluorescence. In vitro, human lung microvascular endothelial cells (HPM...
Source: J Cell Mol Med - November 4, 2020 Category: Molecular Biology Authors: Niu F, Li H, Xu X, Sun L, Gan N, Wang A Tags: J Cell Mol Med Source Type: research

Optimized Langendorff perfusion system for cardiomyocyte isolation in adult mouse heart.
Abstract With the rapid development of single-cell sequencing technology, the Langendorff perfusion system has emerged as a common approach to decompose cardiac tissue and obtain living cardiomyocytes to study cardiovascular disease with the mechanism of cardiomyocyte biology. However, the traditional Langendorff perfusion system is difficult to master, and further, the viability and purity of cardiomyocytes are frequently unable to meet sequencing requirements due to complicated devices and manipulate processes. Here, we provide an optimized Langendorff perfusion system with a simplified and standardized operatin...
Source: J Cell Mol Med - November 4, 2020 Category: Molecular Biology Authors: Li H, Liu C, Bao M, Liu W, Nie Y, Lian H, Hu S Tags: J Cell Mol Med Source Type: research