Overview of RAW264.7 for osteoclastogensis study: Phenotype and stimuli.
Abstract Bone homeostasis is preserved by the balance of maintaining between the activity of osteogenesis and osteoclastogenesis. However, investigations for the osteoclastogenesis were hampered by considerable difficulties associated with isolating and culturing osteoclast in vivo. As the alternative, stimuli-induced osteoclasts formation from RAW264.7 cells (RAW-OCs) have gain its importance for extensively osteoclastogenic study of bone diseases, such as rheumatoid arthritis, osteoporosis, osteolysis and periodontitis. However, considering the RAW-OCs have not yet been well-characterized and RAW264.7 cells are ...
Source: J Cell Mol Med - March 20, 2019 Category: Molecular Biology Authors: Kong L, Smith W, Hao D Tags: J Cell Mol Med Source Type: research

A novel splicing mutation in the PRPH2 gene causes autosomal dominant retinitis pigmentosa in a Chinese pedigree.
PMID: 30892800 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 20, 2019 Category: Molecular Biology Authors: Cheng J, Fu J, Zhou Q, Xiang X, Wei C, Yang L, Fu S, Khan MA, Lv H, Fu J Tags: J Cell Mol Med Source Type: research

Apoptotic bodies from endplate chondrocytes enhance the oxidative stress-induced mineralization by regulating PPi metabolism.
This study aimed to investigate the role of apoptotic bodies (Abs) from the oxidative stressed endplate chondrocytes in regulating mineralization and potential mechanisms. Endplate chondrocytes were isolated from rats and treated with H2O2 to induce oxidative stress. The calcium deposition for matrix mineralization in the cells was examined by histological staining. The expression levels of calcification-related genes in individual groups of cells were determined by quantitative real time-PCR (qRT-PCR). Subsequently, extracellular vesicles (EVs) were purified and characterized. The effect of treatment with H2O2 and/or Abs ...
Source: J Cell Mol Med - March 20, 2019 Category: Molecular Biology Authors: Yuan FL, Xu RS, Ye JX, Zhao MD, Ren LJ, Li X Tags: J Cell Mol Med Source Type: research

FER1L4/miR-372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells.
In this study, it was found that the expression of LncRNA FER1L4 is upregulated in high-grade gliomas than in low-grade cases and that a high expression of LncRNA FER1L4 predicts poor prognosis of gliomas. Meanwhile, in vitro study suggests that expression of FER1L4 with SiRNA knockdown obviously suppresses cell cycle and proliferation. It is further demonstrated by experiments that the FER1L4 knockdown suppresses growth of in vivo glioma. Besides, it is found in our study that LncRNA FER1L4 expression is positively correlated with E2F1 mRNA expression. After knockdown of FER1L4 expression, E2F1 expression is significantly...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Xia L, Nie D, Wang G, Sun C, Chen G Tags: J Cell Mol Med Source Type: research

Inhibition of mitochondrial autophagy protects donor lungs for lung transplantation against ischaemia-reperfusion injury in rats via the mTOR pathway.
Abstract Impaired mitochondrial function is a key factor attributing to lung ischaemia-reperfusion (IR) injury, which contributes to major post-transplant complications. Thus, the current study was performed to investigate the role of mitochondrial autophagy in lung I/R injury and the involvement of the mTOR pathway. We established rat models of orthotopic left lung transplantation to investigate the role of mitochondrial autophagy in I/R injury following lung transplantation. Next, we treated the donor lungs with 3-MA and Rapamycin to evaluate mitochondrial autophagy, lung function and cell apoptosis with differe...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Liu WC, Chen SB, Liu S, Ling X, Xu QR, Yu BT, Tang J Tags: J Cell Mol Med Source Type: research

Egr1 mediates retinal vascular dysfunction in diabetes mellitus via promoting p53 transcription.
CONCLUSION: Down-regulation of Egr1 partially reduced the injurious effects of hyperglycaemia on retinal vascular function via inhibiting p53 expression. PMID: 30887692 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Ao H, Liu B, Li H, Lu L Tags: J Cell Mol Med Source Type: research

PMEPA1 induces EMT via a non-canonical TGF- β signalling in colorectal cancer.
In this study, we first testified that PMEPA1 expresses higher in tumour than normal tissue in Gene Expression Omnibus database, in the Cancer Genome Atlas (TCGA) as well as in the clinical data we collected. Moreover, the higher expression was associated with poor prognosis. We furthermore demonstrated PMEPA1 promotes colorectal cancer metastasis and EMT in vivo and in vitro. We found that PMEPA1 activates the bone morphogenetic proteins (BMP) signalling of TGF-β signalling resulting in promoting EMT and accelerating the proliferation and metastasis of colorectal cancer. PMID: 30887697 [PubMed - as supplied by p...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Zhang L, Wang X, Lai C, Zhang H, Lai M Tags: J Cell Mol Med Source Type: research

circEPSTI1 regulates ovarian cancer progression via decoying miR-942.
Abstract Increasing studies show that circular RNAs (circRNAs) play vital roles in tumour progression. But, how circRNAs function in ovarian cancer is mostly unclear. Here, we detected the expression of circEPSTI1 in ovarian cancer and explored the function of circEPSTI1 in ovarian cancer via a series of experiments. Then, we performed luciferase assay and RNA immunoprecipitation (RIP) assay to explore the competing endogenous RNA (ceRNA) function of circEPSTI1 in ovarian cancer. qRT-PCR verified that circEPSTI1 was overexpressed in ovarian cancer. Inhibition of circEPSTI1 suppressed ovarian cancer cell proliferat...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Xie J, Wang S, Li G, Zhao X, Jiang F, Liu J, Tan W Tags: J Cell Mol Med Source Type: research

FAM3C-YY1 axis is essential for TGF β-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.
In conclusion, FAM3C activated YY1-HSF1 signalling axis to promote the proliferation and migration of breast cancer cells. Furthermore, novel FAM3C-YY1-HSF1 pathway plays an important role in TGFβ-triggered proliferation and migration of human breast cancer MDA-MB-231 cells. PMID: 30887707 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Yang W, Feng B, Meng Y, Wang J, Geng B, Cui Q, Zhang H, Yang Y, Yang J Tags: J Cell Mol Med Source Type: research

The role of NUDT21 in microRNA-binging sites of EZH2 gene increases the of risk preeclampsia.
CONCLUSIONS: This is the first report about the relationship of NUDT21 and EZH2. The data indicate that the aberrant expression of NUDT21 contributes to PE by targeting 3'-UTR of EZH2 mRNA. These findings may provide novel targets for future investigations into therapeutic strategies for PE. PMID: 30883033 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 18, 2019 Category: Molecular Biology Authors: Lang X, Zhao W, Huang D, Liu W, Shen H, Xu L, Xu S, Huang Y, Cheng W Tags: J Cell Mol Med Source Type: research

HC diet inhibited testosterone synthesis by activating endoplasmic reticulum stress in testicular Leydig cells.
Abstract Emerging epidemiological studies indicate that hypercholesterolaemia is a risk factor for testosterone deficiency. However, the underlying mechanism is unclear. Testicular Leydig cells are the primary source of testosterone in males. To identify the effect and mechanism of cholesterol overload on Leydig cell function, rats were fed with a HC (HC) diet to induce hypercholesterolaemia. During the 16-week feeding period, serum testosterone levels were reduced in a time-dependent manner in rats fed the HC diet. Accordingly, these steroidogenic enzymes within the Leydig cells, including steroidogenic acute reg...
Source: J Cell Mol Med - March 18, 2019 Category: Molecular Biology Authors: Yu C, Jiang F, Zhang M, Luo D, Shao S, Zhao J, Gao L, Zuo C, Guan Q Tags: J Cell Mol Med Source Type: research

Inhibition of circular RNA CDR1as increases chemosensitivity of 5-FU-resistant BC cells through up-regulating miR-7.
This study aims to explore the mechanism of Circular RNA CDR1as implicating in regulating 5-fluorouracil (5-FU) chemosensitivity in breast cancer (BC) by competitively inhibiting miR-7 to regulate CCNE1. Expressions of CDR1as and miR-7 in 5-FU-resistant BC cells were determined by RT-PCR. CCK-8, colony formation assay and flow cytometry were applied to measure half maximal inhibitory concentration (IC50), 5-Fu chemosensitivity and cell apoptosis. Western blot was used to detect the expressions of apoptosis-related factors. CDR1as was elevated while miR-7 was inhibited in 5-FU-resistant BC cells. Cells transfected with si-C...
Source: J Cell Mol Med - March 18, 2019 Category: Molecular Biology Authors: Yang W, Gu J, Wang X, Wang Y, Feng M, Zhou D, Guo J, Zhou M Tags: J Cell Mol Med Source Type: research

Ectopic expression of miR-944 impairs colorectal cancer cell proliferation and invasion by targeting GATA binding protein 6.
In conclusion, these findings indicated that miR-944 may be a tumour suppressor and could likely be used as a prognostic predictor and novel therapeutic target for CRC. PMID: 30873717 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 15, 2019 Category: Molecular Biology Authors: Tang JT, Zhao J, Sheng W, Zhou JP, Dong Q, Dong M Tags: J Cell Mol Med Source Type: research

Naftopidil reduced the proliferation of lung fibroblasts and bleomycin-induced lung fibrosis in mice.
In conclusion, naftopidil may have therapeutic effects on lung fibrosis. PMID: 30873733 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 15, 2019 Category: Molecular Biology Authors: Urushiyama H, Terasaki Y, Nagasaka S, Kokuho N, Endo Y, Terasaki M, Kunugi S, Makita K, Isago H, Hosoki K, Souma K, Ishii T, Matsuzaki H, Hiraishi Y, Mikami Y, Noguchi S, Tamiya H, Mitani A, Yamauchi Y, Shimizu A, Nagase T Tags: J Cell Mol Med Source Type: research

CTCF regulates the FoxO signaling pathway to affect the progression of prostate cancer.
In conclusion, CTCF regulates the FoxO signalling pathway to affect the progress of PC. PMID: 30873749 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 15, 2019 Category: Molecular Biology Authors: Shan Z, Li Y, Yu S, Wu J, Zhang C, Ma Y, Zhuang G, Wang J, Gao Z, Liu D Tags: J Cell Mol Med Source Type: research

Anti-cancer targets of formononetin and molecular mechanisms in osteosarcoma: Findings of bioinformatic and experimental assays.
Abstract In current study, a bioinformatic-based network pharmacology was used to identify the osteosarcoma (OGS)-pathological targets and formononetin (FN)-treated targets before the main core predictive biotargets were screened. In addition, all core targets were selected through a number of bioinformatic databases, followed by identification of predominant biological processes and signalling pathways of FN anti-OGS. Further, top three core targets of FN anti-OGS were determined as oestrogen receptor 1 (ESR1), tumour protein p53 (TP53), receptor tyrosine-protein kinase erbB-2 (ERBB2) respectively. In clinical bi...
Source: J Cell Mol Med - March 15, 2019 Category: Molecular Biology Authors: Hu W, Wu X, Tang J, Zhao G, Xiao N, Zhang L, Li S Tags: J Cell Mol Med Source Type: research

Tetraspanin 1 as a mediator of fibrosis inhibits EMT process and Smad2/3 and beta-catenin pathway in human pulmonary fibrosis.
Abstract Tetraspanin 1(TSPAN1) as a clinically relevant gene target in cancer has been studied, but there is no direct in vivo or vitro evidence for pulmonary fibrosis (PF). Using reanalysing Gene Expression Omnibus data, here, we show for the first time that TSPAN1 was markedly down-regulated in lung tissue of patient with idiopathic PF (IPF) and verified the reduced protein expression of TSPAN1 in lung tissue samples of patient with IPF and bleomycin-induced PF mice. The expression of TSPAN1 was decreased and associated with transforming growth factor-β1 (TGF-β1 )-induced molecular characteristics of e...
Source: J Cell Mol Med - March 14, 2019 Category: Molecular Biology Authors: Liu G, Wang Y, Yang L, Zou B, Gao S, Song Z, Lin Z Tags: J Cell Mol Med Source Type: research

The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF- κB p65 translocation.
The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-κB p65 translocation. J Cell Mol Med. 2019 Mar 14;: Authors: Liu YS, Wang LF, Cheng XS, Huo YN, Ouyang XM, Liang LY, Lin Y, Wu JF, Ren JL, Guleng B Abstract Mindin has a broad spectrum of roles in the innate immune system, including in macrophage migration, antigen phagocytosis and cytokine production. Mindin functions as a pattern-recognition molecule for microbial pathogens. However, the underlying mechanisms of mindin-mediated phagocytosis and its exact membrane receptors ...
Source: J Cell Mol Med - March 14, 2019 Category: Molecular Biology Authors: Liu YS, Wang LF, Cheng XS, Huo YN, Ouyang XM, Liang LY, Lin Y, Wu JF, Ren JL, Guleng B Tags: J Cell Mol Med Source Type: research

E-cadherin expression is associated with somatostatin analogue response in acromegaly.
DA, Soto-Moreno A Abstract Acromegaly is a rare disease resulting from hypersecretion of growth hormone (GH) and insulin-like growth factor 1 (IGF1) typically caused by pituitary adenomas, which is associated with increased mortality and morbidity. Somatostatin analogues (SSAs) represent the primary medical therapy for acromegaly and are currently used as first-line treatment or as second-line therapy after unsuccessful pituitary surgery. However, a considerable proportion of patients do not adequately respond to SSAs treatment, and therefore, there is an urgent need to identify biomarkers predictors of response t...
Source: J Cell Mol Med - March 6, 2019 Category: Molecular Biology Authors: Venegas-Moreno E, Flores-Martinez A, Dios E, Vazquez-Borrego MC, Ibañez-Costa A, Madrazo-Atutxa A, Japón MA, Castaño JP, Luque RM, Cano DA, Soto-Moreno A Tags: J Cell Mol Med Source Type: research

HPCAL1 promotes glioblastoma proliferation via activation of Wnt/ β-catenin signalling pathway.
HPCAL1 promotes glioblastoma proliferation via activation of Wnt/β-catenin signalling pathway. J Cell Mol Med. 2019 Mar 06;: Authors: Zhang D, Liu X, Xu X, Xu J, Yi Z, Shan B, Liu B Abstract Glioblastoma (GBM) is the most prevalent primary malignancy of the central nervous system with obvious aggressiveness, and is associated with poor clinical outcome. Studies have indicated that calcium ion (Ca2+ ) can positively regulate the initiation of malignancy with regard to GBM by modulating quiescence, proliferation, migration and maintenance. Hippocalcin like-1 protein (HPCAL1) serves as a sensor of C...
Source: J Cell Mol Med - March 6, 2019 Category: Molecular Biology Authors: Zhang D, Liu X, Xu X, Xu J, Yi Z, Shan B, Liu B Tags: J Cell Mol Med Source Type: research

Increased level of fibrinogen chains in the proteome of blood platelets in secondary progressive multiple sclerosis patients.
This study provides new knowledge of the potential existence of the molecular mechanisms responsible for the acceleration of the platelet pro-coagulant function in SPMS. This can help to identify new targets for therapy, which can then be used not only in the second stage of the disease. PMID: 30838756 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 5, 2019 Category: Molecular Biology Authors: Bijak M, Olejnik A, Rokita B, Morel A, Dziedzic A, Miller E, Saluk-Bijak J Tags: J Cell Mol Med Source Type: research

A molecular overview of the primary dystroglycanopathies.
Abstract Dystroglycan is a major non-integrin adhesion complex that connects the cytoskeleton to the surrounding basement membranes, thus providing stability to skeletal muscle. In Vertebrates, hypoglycosylation of α-dystroglycan has been strongly linked to muscular dystrophy phenotypes, some of which also show variable degrees of cognitive impairments, collectively termed dystroglycanopathies. Only a small number of mutations in the dystroglycan gene, leading to the so called primary dystroglycanopathies, has been described so far, as opposed to the ever-growing number of identified secondary or tertiary dy...
Source: J Cell Mol Med - March 5, 2019 Category: Molecular Biology Authors: Brancaccio A Tags: J Cell Mol Med Source Type: research

Increase in the nuclear localization of PTEN by the Toxoplasma GRA16 protein and subsequent induction of p53-dependent apoptosis and anticancer effect.
This study investigated the efficacy of Toxoplasma GRA16, which binds to herpes virus-associated ubiquitin-specific protease (HAUSP), in anticancer treatment, and whether the expression of GRA16 in genetically modified hepatocellular carcinoma (HCC) cells (GRA16-p53-wild HepG2 and GRA16-p53-null Hep3B) regulates PTEN because alterations in phosphatase and tensin homologue (PTEN) and p53 are vital in liver carcinogenesis and the abnormal p53 gene appears in HCC. For this purpose, we established the GRA16 cell lines using the pBABE retrovirus system, assessed the detailed mechanism of PTEN regulation in vitro and established...
Source: J Cell Mol Med - March 4, 2019 Category: Molecular Biology Authors: Kim SG, Seo SH, Shin JH, Yang JP, Lee SH, Shin EH Tags: J Cell Mol Med Source Type: research

miR-148a inhibits early relapsed colorectal cancers and the secretion of VEGF by indirectly targeting HIF-1 α under non-hypoxia/hypoxia conditions.
This study investigated the role of miR-148a in the regulation of VEGF/angiogenesis and early relapse of CRC. We established a stable clone with miR-148a expression in HCT116 and HT29 cell lines and created a hypoxic condition by using CoCl2 to determine the underlying mechanism of miR-148a. The effects of miR-148a on the phosphoryl-ERK (pERK)/hypoxia-inducible factor-1α (HIF-1α)/VEGF pathway were evaluated through Western blotting and the inhibitory effect of miR-148a on angiogenesis was demonstrated through a tube formation assay. Sixty-three CRC tissues (28 early relapse and 35 non-early relapse) were analys...
Source: J Cell Mol Med - March 4, 2019 Category: Molecular Biology Authors: Tsai HL, Miao ZF, Chen YT, Huang CW, Yeh YS, Yang IP, Wang JY Tags: J Cell Mol Med Source Type: research

Effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryonic development.
In this study, in ovo electroporation was employed in the chicken spinal cord during chicken embryo development. Electroporation was conducted at stage 17 (E2.5), after electroporation the embryos were continued incubating to stage 28 (E6) for sampling, tissue fixation with 4% paraformaldehyde and frozen sectioning. Sonic hedgehog and related protein expressions were detected by in situ hybridization and fluorescence immunohistochemistry and the results were analysed after microphotography. Our results indicate that the ectopic expression of SHH leads to ventralization in the spinal cord during chicken embryonic developmen...
Source: J Cell Mol Med - March 4, 2019 Category: Molecular Biology Authors: Yang C, Li S, Li X, Li H, Li Y, Zhang C, Lin J Tags: J Cell Mol Med Source Type: research

ISG12a and its interaction partner NR4A1 are involved in TRAIL-induced apoptosis in hepatoma cells.
In this study, we found that ISG12a was localized in the mitochondria and nucleus and augmented TRAIL-induced apoptosis through intrinsic apoptotic pathway. In addition, ISG12a interacted with NR4A1 and promoted its nuclear-to-cytoplasm translocation. Upon translocate to cytoplasm, NR4A1 targeted mitochondria and induced Bcl2 conformational change, thereby exposing its BH3 domain. Moreover, TRAIL treatment can induce NR4A1 expression through the activation of NF-κB in TRAIL-resistant Huh7 hepatoma cells. Knockdown of NR4A1 could overcome TRAIL resistance. However, in TRAIL-sensitive LH86 liver cancer cells, TRAIL act...
Source: J Cell Mol Med - March 1, 2019 Category: Molecular Biology Authors: Liu N, Wu Z, Chen A, Chai D, Li L, Zhang L, Zheng J Tags: J Cell Mol Med Source Type: research

A functional interaction between Hippo-YAP signalling and SREBPs mediates hepatic steatosis in diabetic mice.
Abstract The Hippo pathway is an evolutionarily conserved regulator of organ size and tumorigenesis that negatively regulates cell growth and survival. Whether the Hippo pathway regulates cell metabolism is unknown. Here, we report that in the nucleus of hepatocytes, Yes-associated protein(YAP)-the terminal effector of the Hippo pathway-directly interacts with sterol regulatory element binding proteins (SREBP-1c and SREBP-2) on the promoters of the fatty acid synthase (FAS) and 30-hydroxylmethyl glutaryl coenzyme A reductase (HMGCR), thereby stimulating their transcription and promoting hepatocyte lipogenesis and ...
Source: J Cell Mol Med - March 1, 2019 Category: Molecular Biology Authors: Shu Z, Gao Y, Zhang G, Zhou Y, Cao J, Wan D, Zhu X, Xiong W Tags: J Cell Mol Med Source Type: research

Glycyrrhizin protects against sodium iodate-induced RPE and retinal injury though activation of AKT and Nrf2/HO-1 pathway.
This study investigated the effects of glycyrrhizin on retinal pigment epithelium (RPE) in vitro and retina of mice in vivo treated with sodium iodate (SI). Glycyrrhizin significantly inhibited SI-induced reactive oxygen species (ROS), and decreased apoptosis of RPE in vitro. The underlying mechanisms included increased phosphorylation of Akt, and increased expression of nuclear factor erythroid 2-related factor2 (Nrf-2) and HO-1, thereby protecting RPE from SI-induced ROS and apoptosis. Furthermore, glycyrrhizin significantly decreased the apoptosis of retinal cells in vivo, resulting in the inhibition of thinning of reti...
Source: J Cell Mol Med - March 1, 2019 Category: Molecular Biology Authors: He H, Wei D, Liu H, Zhu C, Lu Y, Ke Z, Jiang S, Huang J Tags: J Cell Mol Med Source Type: research

Long non-coding RNA ZEB2-AS1 promotes the proliferation, metastasis and epithelial mesenchymal transition in triple-negative breast cancer by epigenetically activating ZEB2.
In this study, we found that LncRNA-ZEB2-AS1 was dramatically up-regulated in our breast cancer specimens and cells (MDA231), especially in metastatic tumor specimens and highly invasive cells, and high lncRNA-ZEB2-AS1 expression is associated with clinicopathologic features and short survival of breast cancer patients. LncRNA-ZEB2-AS1 promotes the proliferation and metastasis of MDA231 cells in SCID mice. Thus, it is regarded as an oncogene in triple-negative breast cancer. It is mainly endo-nuclear and situated near ZEB2, positively regulating ZEB2 expression and activating the epithelial mesenchymal transition via the P...
Source: J Cell Mol Med - March 1, 2019 Category: Molecular Biology Authors: Zhang G, Li H, Sun R, Li P, Yang Z, Liu Y, Wang Z, Yang Y, Yin C Tags: J Cell Mol Med Source Type: research

Complement C3a promotes proliferation, migration and stemness in cutaneous squamous cell carcinoma.
CONCLUSIONS: C3a enhanced cell proliferation, migration and stemness in cSCC, and this activity was correlated with activation of the Wnt and β-catenin pathway. PMID: 30825266 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 1, 2019 Category: Molecular Biology Authors: Fan Z, Qin J, Wang D, Geng S Tags: J Cell Mol Med Source Type: research

Establishment and characterization of a radiation-induced dermatitis rat model.
In this study, a single dose of 90 Gy irradiation to rats resulted in ulceration, dermal thickening, inflammation, hair follicle loss, and sebaceous glands loss, indicating successful establishment of the model. Few hair follicle cells migrated to form epidermal cells, and both the severity of skin fibrosis and hydroxyproline levels increased with time post-irradiation. Radiation damaged the mitochondria and induced both apoptosis and autophagy of the skin cells. Therefore, irradiation of 90 Gy can be used to successfully establish a rat model of radiation-induced dermatitis. This model will be helpful for develo...
Source: J Cell Mol Med - February 28, 2019 Category: Molecular Biology Authors: Sheng X, Zhou Y, Wang H, Shen Y, Liao Q, Rao Z, Deng F, Xie L, Yao C, Mao H, Liu Z, Peng M, Long Y, Zeng Y, Xue L, Gao N, Kong Y, Zhou X Tags: J Cell Mol Med Source Type: research

Nrf2 promotes breast cancer cell migration via up-regulation of G6PD/HIF-1 α/Notch1 axis.
In this study, we found that the overexpression of Nrf2 promoted proliferation and migration of breast cancers cells. Inhibition of Nrf2 and overexpression of Kelch-like ECH-associated protein 1 (Keap1) reduced the expression of glucose-6-phosphate dehydrogenase (G6PD) and transketolase of pentose phosphate pathway, and overexpression of Nrf2 and knockdown of Keap1 had opposite effects. Our results further showed that the overexpression of Nrf2 promoted the expression of G6PD and Hypoxia-inducing factor 1α (HIF-1α) in MCF-7 and MDA-MB-231 cells. Overexpression of Nrf2 up-regulated the expression of Notch1 via G...
Source: J Cell Mol Med - February 26, 2019 Category: Molecular Biology Authors: Zhang HS, Zhang ZG, Du GY, Sun HL, Liu HY, Zhou Z, Gou XM, Wu XH, Yu XY, Huang YH Tags: J Cell Mol Med Source Type: research

BRD4 inhibition attenuates inflammatory response in microglia and facilitates recovery after spinal cord injury in rats.
In this study, we found that the expression of BRD4 was correlated with pro-inflammatory cytokines after SCI in rats; in vitro study in microglia showed that BRD4 inhibition either by lentivirus or JQ1 may both suppress the MAPK and NF-κB signalling pathways, which are the two major signalling pathways involved in inflammatory response in microglia. BRD4 inhibition by JQ1 not only blocked microglial M1 polarization, but also repressed the level of pro-inflammatory cytokines in microglia in vitro and in vivo. Furthermore, BRD4 inhibition by JQ1 can improve functional recovery and structural disorder as well as reduce ...
Source: J Cell Mol Med - February 26, 2019 Category: Molecular Biology Authors: Wang J, Chen J, Jin H, Lin D, Chen Y, Chen X, Wang B, Hu S, Wu Y, Wu Y, Zhou Y, Tian N, Gao W, Wang X, Zhang X Tags: J Cell Mol Med Source Type: research

Small non-coding RNA and colorectal cancer.
Abstract Colorectal cancer (CRC) is the third most common malignance. Although great efforts have been made to understand the pathogenesis of CRC, the underlying mechanisms are still unclear. It is now clear that more than 90% of the total genome is actively transcribed, but lack of protein-coding potential. The massive amount of RNA can be classified as housekeeping RNAs (such as ribosomal RNAs, transfer RNAs) and regulatory RNAs (such as microRNAs [miRNAs], PIWI-interacting RNA [piRNAs], tRNA-derived stress-induced RNA, tRNA-derived small RNA [tRFs] and long non-coding RNAs [lncRNAs]). Small non-coding RNAs are ...
Source: J Cell Mol Med - February 23, 2019 Category: Molecular Biology Authors: Chen H, Xu Z, Liu D Tags: J Cell Mol Med Source Type: research

MicroRNA-383 inhibits doxorubicin resistance in hepatocellular carcinoma by targeting eukaryotic translation initiation factor 5A2.
Abstract Drug resistance occurs commonly in cancers, especially in hepatocellular carcinoma (HCC). Accumulating evidence has demonstrated that microRNAs (miRNAs) play a vital role in tumour chemoresistance. However, little is known about the role of miR-383 in HCC chemoresistance. In the present study, RT-PCR and western blotting were used to identify the expression profile of miR-383 and eukaryotic translation initiation factor 5A2 (EIF5A2). The bioinformatics website Targetscan was used to predict the target genes of miR-383. In vitro and in vivo loss- and gain-of-function studies were performed to reveal t...
Source: J Cell Mol Med - February 23, 2019 Category: Molecular Biology Authors: Tu C, Chen W, Wang S, Tan W, Guo J, Shao C, Wang W Tags: J Cell Mol Med Source Type: research

The role of LINC00094/miR-224-5p (miR-497-5p)/Endophilin-1 axis in Memantine mediated protective effects on blood-brain barrier in AD microenvironment.
This study was performed to demonstrate the role of the MEM in regulating BBB permeability in AD microenvironment as well as its possible mechanisms. The present study showed that LINC00094 was dramatically increased in Abeta1-42 -incubated microvascular endothelial cells (ECs) of BBB model in vitro. Besides, it was decreased in MEM-incubated ECs. Silencing LINC00094 significantly decreased BBB permeability, meanwhile up-regulating the expression of ZO-1, occludin and claudin-5. Furthermore, silencing LINC00094 enhance the effect of MEM on decreasing BBB permeability in AD microenvironment. The analysis of the mechanism de...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Zhu L, Lin M, Ma J, Liu W, Gao L, Wei S, Xue Y, Shang X Tags: J Cell Mol Med Source Type: research

IL-35Ig-expressing dendritic cells induce tolerance via Arginase 1.
Abstract The cytokine interleukin IL-35 is known to exert strong immunosuppressive functions. Indoleamine 2,3-dioxygenase 1 (IDO1) and Arginase 1 (Arg1) are metabolic enzymes that, expressed by dendritic cells (DCs), contribute to immunoregulation. Here, we explored any possible link between IL-35 and the activity of those enzymes. We transfected a single chain IL-35Ig gene construct in murine splenic DCs (DC35 ) and assessed any IDO1 and Arg1 activities as resulting from ectopic IL-35Ig expression, both in vitro and in vivo. Unlike Ido1, Arg1 expression was induced in vitro in DC35 , and it conferred an immunosup...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Panfili E, Mondanelli G, Orabona C, Bianchi R, Gargaro M, Fallarino F, Puccetti P, Grohmann U, Volpi C, Belladonna ML Tags: J Cell Mol Med Source Type: research

Down-regulated lncRNA SLC25A5-AS1 facilitates cell growth and inhibits apoptosis via miR-19a-3p/PTEN/PI3K/AKT signalling pathway in gastric cancer.
In this study, we investigate the roles of lncRNA SLC25A5-AS1 on tumourigenesis and explore its potential mechanisms in GC. The results showed that the expressions of SLC25A5-AS1 in GC were significantly lower than that of adjacent normal tissues, which were significantly associated with tumour size, TNM stage and lymph node metastasis. Moreover, SLC25A5-AS1 could inhibit GC cell proliferation, induce G1/G1 cell cycle arrest and cell apoptosis in vitro, as well as GC growth in vivo. Dual-luciferase reporter assay confirmed the direct interaction between SLC25A5-AS1 and miR-19a-3p, rescue experiment showed that co-transfect...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Li X, Yan X, Wang F, Yang Q, Luo X, Kong J, Ju S Tags: J Cell Mol Med Source Type: research

Inhibition of P53/miR-34a improves diabetic endothelial dysfunction via activation of SIRT1.
Abstract Endothelial dysfunction contributes to diabetic macrovascular complications, resulting in high mortality. Recent findings demonstrate a pathogenic role of P53 in endothelial dysfunction, encouraging the investigation of the effect of P53 inhibition on diabetic endothelial dysfunction. Thus, high glucose (HG)-treated endothelial cells (ECs) were subjected to pifithrin-α (PFT-α)-a specific inhibitor of P53, or P53-small interfering RNA (siRNA), both of which attenuated the HG-induced endothelial inflammation and oxidative stress. Moreover, inhibition of P53 by PFT-α or P53-siRNA prohibited...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Wu J, Liang W, Tian Y, Ma F, Huang W, Jia Y, Jiang Z, Wu H Tags: J Cell Mol Med Source Type: research

A novel role of HuR in -Epigallocatechin-3-gallate (EGCG) induces tumour cells apoptosis.
PMID: 30793483 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Jian W, Fang S, Chen T, Fang J, Mo Y, Li D, Xiong S, Liu W, Song L, Shen J, Xia Y, Wang Q, Hong H Tags: J Cell Mol Med Source Type: research

MARCH1 encourages tumour progression of hepatocellular carcinoma via regulation of PI3K-AKT- β-catenin pathways.
MARCH1 encourages tumour progression of hepatocellular carcinoma via regulation of PI3K-AKT-β-catenin pathways. J Cell Mol Med. 2019 Feb 22;: Authors: Xie L, Dai H, Li M, Yang W, Yu G, Wang X, Wang P, Liu W, Hu X, Zhao M Abstract Membrane-associated RING-CH-1 (MARCH1) is a membrane-anchored E3 ubiquitin ligase that is involved in a variety of cellular processes. MARCH1 was aberrantly expressed as a tumour promoter in ovarian cancer, but the signalling about the molecular mechanism has not yet been fully illuminated. Here, we first determined that MARCH1 was obviously highly expressed in human hep...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Xie L, Dai H, Li M, Yang W, Yu G, Wang X, Wang P, Liu W, Hu X, Zhao M Tags: J Cell Mol Med Source Type: research

Establishment and molecular characterization of decitabine-resistant K562 cells.
In this study, by gradually increasing concentration after a continuous induction of DAC, we established the DAC-resistant K562 cell line (K562/DAC) from its parental cell line K562. The proliferation and survival rate of K562/DAC was significantly increased, whereas the apoptosis rate was remarkably decreased than that of K562 after DAC treatment. In K562/DAC, a total of 108 genes were upregulated and 118 genes were downregulated by RNA-Seq. In addition, we also observed aberrant expression of DDX43/H19/miR-186 axis (increased DDX43/H19 and decreased miR-186) in K562/DAC cells. Ectopic expression of DDX43 in parental K562...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Wen XM, Zhang TJ, Ma JC, Zhou JD, Xu ZJ, Zhu XW, Yuan Q, Ji RB, Chen Q, Deng ZQ, Lin J, Qian J Tags: J Cell Mol Med Source Type: research

Acid-sensing ion channel 1a is involved in ischaemia/reperfusion induced kidney injury by increasing renal epithelia cell apoptosis.
Abstract Acidic microenvironment is commonly observed in ischaemic tissue. In the kidney, extracellular pH dropped from 7.4 to 6.5 within 10 minutes initiation of ischaemia. Acid-sensing ion channels (ASICs) can be activated by pH drops from 7.4 to 7.0 or lower and permeates to Ca2+ entrance. Thus, activation of ASIC1a can mediate the intracellular Ca2+ accumulation and play crucial roles in apoptosis of cells. However, the role of ASICs in renal ischaemic injury is unclear. The aim of the present study was to test the hypothesis that ischaemia increases renal epithelia cell apoptosis through ASIC1a-mediated ...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Song N, Lu Z, Zhang J, Shi Y, Ning Y, Chen J, Jin S, Shen B, Fang Y, Zou J, Teng J, Chu XP, Shen L, Ding X Tags: J Cell Mol Med Source Type: research

β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury.
β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury. J Cell Mol Med. 2019 Feb 22;: Authors: Chen L, Kong L, Wei X, Wang Y, Wang B, Zhang X, Sun J, Liu H Abstract We previously reported that nucleotide-binding oligomerization domain-containing protein (NOD) 2 was involved in the inflammatory responses to cerebral ischaemia/reperfusion (I/R) insult. However, the mechanism by which NOD2 participates in brain ischaemic injury and the regulation of NOD2 in the process are still obscure. Increased β-arres...
Source: J Cell Mol Med - February 22, 2019 Category: Molecular Biology Authors: Chen L, Kong L, Wei X, Wang Y, Wang B, Zhang X, Sun J, Liu H Tags: J Cell Mol Med Source Type: research

Inhibition of the amino-acid transporter LAT1 demonstrates anti-neoplastic activity in medulloblastoma.
In this study, we investigated the therapeutic potential of JPH203, a LAT1-specific pharmacological inhibitor, on two independent MB cell lines belonging to subgroups 3 (HD-MB03) and Shh (DAOY). We show that while displaying low toxicity towards normal cerebral cells, JPH203 disrupts AA homeostasis, mTORC1 activity, proliferation and survival in MB cells. Moreover, we demonstrate that a long-term treatment with JPH203 does not lead to resistance in MB cells. Therefore, this study suggests that targeting LAT1 with JPH203 is a promising therapeutic approach for MB treatment. PMID: 30784173 [PubMed - as supplied by publi...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Cormerais Y, Pagnuzzi-Boncompagni M, Schrötter S, Giuliano S, Tambutté E, Endou H, Wempe MF, Pagès G, Pouysségur J, Picco V Tags: J Cell Mol Med Source Type: research

The role of dendritic cells regulated by HMGB1/TLR4 signalling pathway in myocardial ischaemia reperfusion injury.
Abstract Inflammatory response plays an important role in ischaemia reperfusion injury (IRI) through a variety of inflammatory cells. Apart from neutrophils, macrophages and lymphocytes, the role of dendritic cells (DCs) in IRI has been noticed. The study was aimed at investigating whether the high-mobility group protein box-1/toll like receptor 4 (HMGB1/TLR4) signalling pathway regulate the migration, adhesion and aggregation of DCs to the myocardium, induce DCs activation and maturation, stimulate the expression of surface costimulatory molecules and participate in myocardial IRI. In vivo, migration, adhesion, a...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Xue J, Ge H, Lin Z, Wang H, Lin W, Liu Y, Wu G, Xia J, Zhao Q Tags: J Cell Mol Med Source Type: research

Notch1 activates angiogenic regulator Netrin4 in endothelial cells.
Abstract Netrin4 (NTN4) is a chemotropic factor that regulates angiogenesis. We found that endothelial expression of the activated, intracellular domain of Notch1 (NICD1), significantly up-regulated NTN4 mRNA as well as intracellular NTN4 protein in both transgenic mice and cultured human umbilical vein endothelial cells (HUVECs). Notch1 activation also increased NTN4 secretion from HUVECs. We subsequently demonstrated that NICD1 bound to CSL (CBF1, Suppressor of Hairless, Lag-1), a core component of Notch transcription complex, at the -53 element of the human NTN4 gene promoter. Loss of the -53 element compromise...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Liu Q, Allen TD, Song W, Wada Y, Lobe CG, Liu J Tags: J Cell Mol Med Source Type: research

Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy.
Abstract Diabetic nephropathy (DN) is characterized by inflammation of renal tissue. Glomerular endothelial cells (GEnCs) play an important role in inflammation and protein leakage in urine in DN patients. Chemerin and its receptor ChemR23 are inducers of inflammation. The aim of this study was to investigate the function of chemerin/ChemR23 in GEnCs of DN patients. Immunohistochemical staining and qRT-PCR were used to measure the expression of chemerin, ChemR23 and inflammatory factors in renal tissues of DN patients. Db/db mice were used as animal model. ChemR23 of DN mice was knocked down by injecting LV3-shRNA...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Shang J, Wang L, Zhang Y, Zhang S, Ning L, Zhao J, Cheng G, Liu D, Xiao J, Zhao Z Tags: J Cell Mol Med Source Type: research

Predicting overall survival of patients with hepatocellular carcinoma using a three-category method based on DNA methylation and machine learning.
In this study, we analyzed 450K methylation chip data from 377 HCC samples and 50 adjacent normal samples in the TCGA database. We screened 47,099 differentially methylated sites using Cox regression as well as SVM-RFE and FW-SVM algorithms, and constructed a model using three risk categories to predict the overall survival based on 134 methylation sites. The model showed a 10-fold cross-validation score of 0.95 and satisfactory predictive power, and correctly classified 26 of 33 samples in testing set obtained by stratified sampling from high, intermediate and low risk groups. PMID: 30784182 [PubMed - as supplied by ...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Dong RZ, Yang X, Zhang XY, Gao PT, Ke AW, Sun HC, Zhou J, Fan J, Cai JB, Shi GM Tags: J Cell Mol Med Source Type: research

The pathogenesis of endemic fluorosis: Research progress in the last 5  years.
The pathogenesis of endemic fluorosis: Research progress in the last 5 years. J Cell Mol Med. 2019 Feb 19;: Authors: Wei W, Pang S, Sun D Abstract Fluorine is one of the trace elements necessary for health. It has many physiological functions, and participates in normal metabolism. However, fluorine has paradoxical effects on the body. Many studies have shown that tissues and organs of humans and animals appear to suffer different degrees of damage after long-term direct or indirect exposure to more fluoride than required to meet the physiological demand. Although the aetiology of endemic fluoros...
Source: J Cell Mol Med - February 19, 2019 Category: Molecular Biology Authors: Wei W, Pang S, Sun D Tags: J Cell Mol Med Source Type: research