Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus.
In conclusion, we provide evidence that iPSCs reprogrammed from CNS-SLE patient are a powerful useful tool to investigate the molecular mechanisms underlying the disease and to eventually develop innovative therapeutic approaches. PMID: 31536674 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 19, 2019 Category: Molecular Biology Authors: De Angelis MT, Santamaria G, Parrotta EI, Scalise S, Lo Conte M, Gasparini S, Ferlazzo E, Aguglia U, Ciampi C, Sgura A, Cuda G Tags: J Cell Mol Med Source Type: research
Helicobacter pylori recrudescence and its influencing factors.
This study reviewed the recrudescence of H pylori infection and its influencing factors. PMID: 31536675 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 19, 2019 Category: Molecular Biology Authors: Sun Y, Zhang J Tags: J Cell Mol Med Source Type: research
A gain of function paradox: Targeted therapy for glioblastoma associated with abnormal NHE9 expression.
Abstract Glioblastoma (GBM) is the most frequent and inevitably lethal primary brain cancer in adults. It is recognized that the overexpression of the endosomal Na+ /H+ exchanger NHE9 is a potent driver of GBM progression. Patients with NHE9 overexpression have a threefold lower median survival relative to GBM patients with normal NHE9 expression, using available treatment options. New treatment strategies tailored for this GBM subset are much needed. According to the prevailing model, NHE9 overexpression leads to an increase in plasma membrane density of epidermal growth factor receptors (EGFRs) which consequentl...
Source: J Cell Mol Med - September 18, 2019 Category: Molecular Biology Authors: Pall AE, Juratli L, Guntur D, Bandyopadhyay K, Kondapalli KC Tags: J Cell Mol Med Source Type: research
Iron chelation and 2-oxoglutarate-dependent dioxygenase inhibition suppress mantle cell lymphoma's cyclin D1.
Abstract The patients with mantle cell lymphoma (MCL) have translocation t(11;14) associated with cyclin D1 overexpression. We observed that iron (an essential cofactor of dioxygenases including prolyl hydroxylases [PHDs]) depletion by deferoxamine blocked MCL cells' proliferation, increased expression of DNA damage marker γH2AX, induced cell cycle arrest and decreased cyclin D1 level. Treatment of MCL cell lines with dimethyloxalylglycine, which blocks dioxygenases involving PHDs by competing with their substrate 2-oxoglutarate, leads to their decreased proliferation and the decrease of cyclin D1 level. We ...
Source: J Cell Mol Med - September 13, 2019 Category: Molecular Biology Authors: Babosova O, Kapralova K, Raskova Kafkova L, Korinek V, Divoky V, Prchal JT, Lanikova L Tags: J Cell Mol Med Source Type: research
FOXC1 up-regulates the expression of toll-like receptors in myocardial ischaemia.
Abstract Myocardial ischaemia (MI) remains a major cause of death and disability worldwide. Accumulating evidence suggests a significant role for innate immunity, in which the family of toll-like receptors (TLRs) acts as an essential player. We previously reported and reviewed the changes of Tlr expression in models of MI. However, the underlying mechanisms regulating Tlr expression in MI remain unclear. The present study first screened transcription factors (TFs) that potentially regulate Tlr gene transcription based on in silico analyses followed by experimental verification, using both in vivo and in vitro mode...
Source: J Cell Mol Med - September 13, 2019 Category: Molecular Biology Authors: Zhang SP, Yang RH, Shang J, Gao T, Wang R, Peng XD, Miao X, Pan L, Yuan WJ, Lin L, Hu QK Tags: J Cell Mol Med Source Type: research
PFN1 and integrin- β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells.
In this study we postulate that pfn-1 knock down promotes epithelial lineage by inhibiting the integrin-β1(CD29)/mTOR pathway and subsequent NANOG down-expression. We showed that it is possible modulate pfn1 expression levels by treating f-LSCs with Resveratrol (RSV), a natural compound: pfn1 decline is accompanied with up-regulation of the specific differentiation epithelial genes pax6 (paired-box 6), sox17 (sex determining region Y-box 17) and ΔNp63-α (p63 splice variant), consistent with drop-down of the principle stem gene levels. These results contribute to understand the molecular biology of corneal ...
Source: J Cell Mol Med - September 12, 2019 Category: Molecular Biology Authors: Tomasello L, Coppola A, Pitrone M, Failla V, Cillino S, Pizzolanti G, Giordano C Tags: J Cell Mol Med Source Type: research
LncRNA SNHG14 potentiates pancreatic cancer progression via modulation of annexin A2 expression by acting as a competing endogenous RNA for miR-613.
This study aimed to determine long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) expression in pancreatic cancer and to explore the potential molecular actions of SNHG14 in mediating pancreatic cancer progression. Gene expression was detected by quantitative real-time PCR. Cell proliferation, growth and invasion were detected by respective CCK-8, colony formation, and transwell invasion assays. Protein levels were measured by Western blotting. Cell apoptosis and caspase-3 activity were detected by flow cytometry and caspase-3 activity assay. The link between miR-613 and its targets was evaluated by luci...
Source: J Cell Mol Med - September 12, 2019 Category: Molecular Biology Authors: Deng PC, Chen WB, Cai HH, An Y, Wu XQ, Chen XM, Sun DL, Yang Y, Shi LQ, Yang Y Tags: J Cell Mol Med Source Type: research
EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer.
Abstract EGR1 regulates the expression of its downstream target genes and may exert different biological effects in different tumours. We found that the expression of EGR1 was increased in gastric cancer (GC), and silencing the expression of EGR1 promoted the apoptosis of GC cells. Moreover, overexpression of EGR1 repressed the apoptosis of GC cells. Bioinformatics analysis showed that EGR1 had binding sites at the upstream promoter region of miR-195; ChIP assays were applied to determine EGR1 occupancy of the miR-195 promoter. The RT-PCR results showed that EGR1 suppressed the expression of miR-195. The mechanism...
Source: J Cell Mol Med - September 12, 2019 Category: Molecular Biology Authors: Yang Y, Wu F, Zhang J, Sun R, Li F, Li Y, Chang S, Wang L, Wang X, Liu L, Huang C Tags: J Cell Mol Med Source Type: research
Biphasic effects of autophagy on decompression bubble-induced endothelial injury.
In conclusion, autophagy was involved and played a biphasic role in bubble-induced endothelial injury. PMID: 31515946 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 12, 2019 Category: Molecular Biology Authors: Wang M, Zhang K, Nie S, Huang G, Yi H, He C, Buzzacott P, Xu W Tags: J Cell Mol Med Source Type: research
LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP-1 signalling-mediated RUNX2 expression.
In this study, we identify lncRNA HOTAIRM1 as a critical regulator to promote osteogenesis of MSCs. Loss of HOTAIRM1 significantly inhibits the calcium deposition and alkaline phosphatase activity of MSCs. Mechanistically, we find that HOTAIRM1 positively modulates the activity of JNK and c-Jun, both of which are widely accepted as crucial regulators of osteogenic differentiation. More importantly, c-Jun is found to be functionally involved in the regulation of RUNX2 expression, a master transcription factor of osteogenesis. In detail, c-Jun can help recruit the acetyltransferase p300 to RUNX2 promoter, facilitating acetyl...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Fu L, Peng S, Wu W, Ouyang Y, Tan D, Fu X Tags: J Cell Mol Med Source Type: research
Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition.
Abstract The role of Non-POU-domain-containing octamer-binding protein (NONO) in the formation and development of angiotensin II (Ang II)-induced abdominal aortic aneurysm (AAA) in apolipoprotein E-knockout (ApoE-/- ) mice is still unknown. In Part I, the protein level of NONO was suggestively greater in the AAA tissues compare to that in the normal abdominal aortas. In Part II, 20 ApoE-/- male mice were used to examine the transfection efficiency of lentivirus by detecting GFP fluorescence. In Part III, mice were arbitrarily separated into two groups: one was the control group without Ang II infusion, and another...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Xu X, Zhang F, Lu Y, Yu S, Sun W, Sun S, Cheng J, Ma J, Zhang M, Zhang C, Zhang Y, Zhang K Tags: J Cell Mol Med Source Type: research
Mesenchymal stem cell-derived exosomes ameliorate erection by reducing oxidative stress damage of corpus cavernosum in a rat model of artery injury.
Abstract Erectile dysfunction (ED) is a common ageing male's disease, and vascular ED accounts for the largest proportion of all types of ED. One of the mechanisms of vascular ED in the clinic is arterial insufficiency, which mainly caused by atherosclerosis, trauma and surgical. Moreover, oxidative stress damage after tissue ischemia usually aggravated the progress of ED. As a new way of acellular therapy, mesenchymal stem cell-derived exosomes (MSC-Exos) have great potential in ED treatment. In the current study, we have explored the mechanism of MSC-Exos therapy in a rat model of internal iliac artery injury-in...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Liu Y, Zhao S, Luo L, Wang J, Zhu Z, Xiang Q, Deng Y, Zhao Z Tags: J Cell Mol Med Source Type: research
Cardiac function modulation depends on the A-kinase anchoring protein complex.
Abstract The A-kinase anchoring proteins (AKAPs) are a group of structurally diverse proteins identified in various species and tissues. These proteins are able to anchor protein kinase and other signalling proteins to regulate cardiac function. Acting as a scaffold protein, AKAPs ensure specificity in signal transduction by enzymes close to their appropriate effectors and substrates. Over the decades, more than 70 different AKAPs have been discovered. Accumulative evidence indicates that AKAPs play crucial roles in the functional regulation of cardiac diseases, including cardiac hypertrophy, myofibre contractilit...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Zhu YR, Jiang XX, Zheng Y, Xiong J, Wei D, Zhang DM Tags: J Cell Mol Med Source Type: research
Pristimerin attenuates cell proliferation of uveal melanoma cells by inhibiting insulin-like growth factor-1 receptor and its downstream pathways.
Abstract Uveal melanoma (UM) has a high mortality rate due to liver metastasis. The insulin-like growth factor-1 receptor (IGF-1R) is highly expressed in UM and has been shown to be associated with hepatic metastases. Targeting IGF signalling may be considered as a promising approach to inhibit the process of metastatic UM cells. Pristimerin (PRI) has been demonstrated to inhibit the growth of several cancer cells, but its role and underlying mechanisms in the IGF-1-induced UM cell proliferation are largely unknown. The present study examined the anti-proliferative effect of PRI on UM cells and its possible role i...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Xie X, Xie S, Xie C, Fang Y, Li Z, Wang R, Jiang W Tags: J Cell Mol Med Source Type: research
Effects of autophagy on apoptosis of articular chondrocytes in adjuvant arthritis rats.
In this study, we explored the effect of autophagy on apoptosis of articular chondrocytes in vivo and in vitro. Adjuvant arthritis (AA) and acid-induced primary articular chondrocyte apoptosis were used as in vivo and in vitro models, respectively. Articular chondrocyte autophagy and apoptosis were both observed dynamically in AA rat articular cartilage at different stages (15 days, 25 days and 35 days). Moreover, chondrocyte apoptosis and articular cartilage injury in AA rats were increased by the autophagy inhibitor 3-methyladenine (3-MA) and decreased by the autophagy activator rapamycin. In addition, pre...
Source: J Cell Mol Med - September 11, 2019 Category: Molecular Biology Authors: Zhou R, Zhu F, Wu X, Song S, Chen Y, Zhu C, Dai B, Qian X, Wang K, Hu W, Chen F Tags: J Cell Mol Med Source Type: research
Icariin promotes angiogenesis in glucocorticoid-induced osteonecrosis of femoral heads: In vitro and in vivo studies.
This study revealed that ICA promotes angiogenesis of BMECs in vitro and improves femoral head blood vessel volume of rats treated with glucocorticoid, suggesting the efficacy of ICA in the prevention of glucocorticoid-induced ONFH. PMID: 31507078 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 10, 2019 Category: Molecular Biology Authors: Yu H, Yue J, Wang W, Liu P, Zuo W, Guo W, Zhang Q Tags: J Cell Mol Med Source Type: research
Sigma-1 receptor protects against ferroptosis in hepatocellular carcinoma cells.
In conclusion, we demonstrate that S1R protects HCC cells against sorafenib and subsequent ferroptosis. A better understanding of the role of S1R in ferroptosis may provide novel insight into this biological process. PMID: 31507082 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 10, 2019 Category: Molecular Biology Authors: Bai T, Lei P, Zhou H, Liang R, Zhu R, Wang W, Zhou L, Sun Y Tags: J Cell Mol Med Source Type: research
Reciprocal regulation of miR-206 and IL-6/STAT3 pathway mediates IL6-induced gefitinib resistance in EGFR-mutant lung cancer cells.
In this study, we investigated the role of miR-206 in IL6-induced gefitinib-resistant EGFR-mutated lung cancer cell lines. We showed that forced miR-206 expression restored gefitinib sensitivity in IL6-induced gefitinib-resistant EGFR-mutant lung cancer cells by inhibiting IL6/JAK1/STAT3 pathway. Specifically, mechanistic investigations revealed that miR-206 blocked IL-6/STAT3 signalling via directly targeting the 3'-UTR of intracellular IL-6 messenger RNA. Moreover, IL-6 induced miR-206 down-regulation by reducing the cropping process of primary miR-206 (pri-miR-206) into the Drosha/DGCR8 complex. Taken together, our find...
Source: J Cell Mol Med - September 10, 2019 Category: Molecular Biology Authors: Yang Y, Wang W, Chang H, Han Z, Yu X, Zhang T Tags: J Cell Mol Med Source Type: research
Protective role of p120-catenin on mitochondria by inhibiting NLRP3 in ventilator-induced lung injury.
Abstract Mitochondria supply energy to maintain the integrity of cell junctions. NLRP3, as the core component of the inflammatory response, is crucial in mechanical stretching. Mechanical stretching could activate NLRP3 and induce mitochondrial dysfunction. The relationship between p120 and mitochondria in ventilator-induced lung injury (VILI) has not been elucidated. MLE-12 cells and wild-type male C57BL/6 mice were pre-treated with MCC950 (specific and highly efficient inhibitor of NLRP3) or a p120 siRNA-liposome complex. Then, the cells were subjected to 20% cyclic stretching, and the mice were subjected to mec...
Source: J Cell Mol Med - September 10, 2019 Category: Molecular Biology Authors: Liu G, Gu C, Liu M, Liu H, Wang D, Liu X, Wang Y Tags: J Cell Mol Med Source Type: research
GSK-3 β inhibition protects the rat heart from the lipopolysaccharide-induced inflammation injury via suppressing FOXO3A activity.
GSK-3β inhibition protects the rat heart from the lipopolysaccharide-induced inflammation injury via suppressing FOXO3A activity. J Cell Mol Med. 2019 Sep 10;: Authors: Li Z, Zhu H, Liu C, Wang Y, Wang D, Liu H, Cao W, Hu Y, Lin Q, Tong C, Lu M, Sachinidis A, Li L, Peng L Abstract Sepsis-induced cardiac dysfunction represents a main cause of death in intensive care units. Previous studies have indicated that GSK-3β is involved in the modulation of sepsis. However, the signalling details of GSK-3β regulation in endotoxin lipopolysaccharide (LPS)-induced septic myocardial dysfunction are ...
Source: J Cell Mol Med - September 10, 2019 Category: Molecular Biology Authors: Li Z, Zhu H, Liu C, Wang Y, Wang D, Liu H, Cao W, Hu Y, Lin Q, Tong C, Lu M, Sachinidis A, Li L, Peng L Tags: J Cell Mol Med Source Type: research
Uridine phosphorylase 1 associates to biological and clinical significance in thyroid carcinoma cell lines.
Abstract Thyroid cancer incidence has been continuity increasing worldwide. Uridine phosphorylase 1 (UPP1) is a protein-coding gene and has been detected that UPP1 was the higher expression in many solid malignancies, just as head and neck cancers, breast cancer, compared with paired normal tissue. But the act of UPP1 in thyroid cancer is not explicit. In this article, we investigate the function of UPP1 expression in thyroid cancer. The Cancer Genome Atlas (TCGA) unpaired thyroid cancer and normal RNA-seq data were downloaded, and our paired thyroid cancer and normal samples were analysed by a polymerase chain re...
Source: J Cell Mol Med - September 9, 2019 Category: Molecular Biology Authors: Guan Y, Bhandari A, Zhang X, Wang O Tags: J Cell Mol Med Source Type: research
Testis developmental related gene 1 regulates the chemosensitivity of seminoma TCam-2 cells to cisplatin via autophagy.
This study has uncovered a novel role of TDRG1 in reducing chemoresistance during CDDP treatment and provides potential therapeutic strategies for the treatment of human seminoma. PMID: 31496041 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 9, 2019 Category: Molecular Biology Authors: Peng D, Wei J, Gan Y, Yang J, Jiang X, Kitazawa R, Xiang Y, Dai Y, Tang Y Tags: J Cell Mol Med Source Type: research
Schistosoma japonicum peptide SJMHE1 suppresses airway inflammation of allergic asthma in mice.
Abstract Helminths and their products can shape immune responses by modulating immune cells, which are dysfunctional in inflammatory diseases such as asthma. We previously identified SJMHE1, a small molecule peptide from the HSP60 protein of Schistosoma japonicum. SJMHE1 can inhibit delayed-type hypersensitivity and collagen-induced arthritis in mice. In the present study, we evaluated this peptide's potential intervention effect and mechanism on ovalbumin-induced asthma in mice. SJMHE1 treatment suppressed airway inflammation in allergic mice, decreased the infiltrating inflammatory cells in the lungs and broncho...
Source: J Cell Mol Med - September 9, 2019 Category: Molecular Biology Authors: Zhang W, Li L, Zheng Y, Xue F, Yu M, Ma Y, Dong L, Shan Z, Feng D, Wang T, Wang X Tags: J Cell Mol Med Source Type: research
LINC01303 functions as a competing endogenous RNA to regulate EZH2 expression by sponging miR-101-3p in gastric cancer.
Abstract Long non-coding RNA (lncRNA) is one of the important regulators of many malignancies. However, the biological function and clinical significance of a large number of lncRNAs in gastric cancer remain unclear. Therefore, we analysed the TCGA data to find that LINC01303 is significantly up-regulated in gastric cancer tissues. However, the biological function of LINC01303 in GC remains unknown. In our study, we found that the expression of LINC01303 was significantly higher in GC tissues than in adjacent tissues by real-time quantitative PCR. We can significantly inhibit the malignant proliferation, migration...
Source: J Cell Mol Med - September 9, 2019 Category: Molecular Biology Authors: Cao C, Xu Y, Du K, Mi C, Yang C, Xiang L, Xie Y, Liu W Tags: J Cell Mol Med Source Type: research
MCU Up-regulation contributes to myocardial ischemia-reperfusion Injury through calpain/OPA-1-mediated mitochondrial fusion/mitophagy Inhibition.
Abstract Mitochondrial dynamic disorder is involved in myocardial ischemia/reperfusion (I/R) injury. To explore the effect of mitochondrial calcium uniporter (MCU) on mitochondrial dynamic imbalance under I/R and its related signal pathways, a mouse myocardial I/R model and hypoxia/reoxygenation model of mouse cardiomyocytes were established. The expression of MCU during I/R increased and related to myocardial injury, enhancement of mitochondrial fission, inhibition of mitochondrial fusion and mitophagy. Suppressing MCU functions by Ru360 during I/R could reduce myocardial infarction area and cardiomyocyte apoptos...
Source: J Cell Mol Med - September 9, 2019 Category: Molecular Biology Authors: Guan L, Che Z, Meng X, Yu Y, Li M, Yu Z, Shi H, Yang D, Yu M Tags: J Cell Mol Med Source Type: research
Effect of oxidative stress and 3-hydroxytyrosol on DNA methylation levels of miR-9 promoters.
gni F PMID: 31496000 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 8, 2019 Category: Molecular Biology Authors: D'Adamo S, Cetrullo S, Borzì RM, Flamigni F Tags: J Cell Mol Med Source Type: research
The non-coding RNome after splenectomy.
Abstract Splenectomy is a common surgical procedure performed in millions of people worldwide. Epidemiologic data show that splenectomy is followed by infectious (sepsis) and non-infectious complications, with unknown mechanisms. In order to explore the role of the non-coding transcripts involved in these complications, we analysed a panel of circulating microRNAs (miRNAs), which were previously reported to be deregulated in sepsis, in the plasma of splenectomized patients. MiR-223 was overexpressed immediately and late after splenectomy, while miR-146a was overexpressed immediately after splenectomy, returning la...
Source: J Cell Mol Med - September 8, 2019 Category: Molecular Biology Authors: Dragomir MP, Tudor S, Okubo K, Shimizu M, Chen M, Giza DE, He WR, Ivan C, Calin GA, Vasilescu C Tags: J Cell Mol Med Source Type: research
Sodium hydrosulphide restores tumour necrosis factor- α-induced mitochondrial dysfunction and metabolic dysregulation in HL-1 cells.
This study investigated whether H2 S modulates TNF-α-dysregulated mitochondrial function and metabolism in cardiomyocytes. HL-1 cells were incubated with TNF-α (25 ng/mL) with or without sodium hydrosulphide (NaHS, 0.1 mmol/L) for 24 hours. Cardiac peroxisome proliferator-activated receptor (PPAR) isoforms, pro-inflammatory cytokines, receptor for advanced glycation end products (RAGE) and fatty acid metabolism were evaluated through Western blotting. The mitochondrial oxygen consumption rate and adenosine triphosphate (ATP) production were investigated using Seahorse XF24 extracellular flux anal...
Source: J Cell Mol Med - September 8, 2019 Category: Molecular Biology Authors: Lee TI, Kao YH, Baigalmaa L, Lee TW, Lu YY, Chen YC, Chao TF, Chen YJ Tags: J Cell Mol Med Source Type: research
The N-terminal D1 domain of Treponema pallidum flagellin binding to TLR5 is required but not sufficient in activation of TLR5.
Abstract Syphilis is a chronic bacterial infection caused by Treponema pallidum (T pallidum) and the pathogenesis that T pallidum infection induces immunopathological damages in skin and other tissues remains unclear. We have previously reported that recombinant flagellins of T pallidum can elicit IL-6 and IL-8 transcriptions via TLR5 pathway. To identify the domains which induced the pro-inflammatory activity and the importance of the interactions between TLR5 and domains, homology-based modelling and comparative structural analyses revealed that Tpflagellins can combine with TLR5 directly. Deletio...
Source: J Cell Mol Med - September 7, 2019 Category: Molecular Biology Authors: Xu M, Xie Y, Tan M, Zheng K, Xiao Y, Jiang C, Zhao F, Zeng T, Wu Y Tags: J Cell Mol Med Source Type: research
miR-20a-5p regulates pulmonary surfactant gene expression in alveolar type II cells.
Abstract MicroRNA (miRNA) critically controls gene expression in many biological processes, including lung growth and pulmonary surfactant biosynthesis. The present study was conducted to investigate whether miR-20a-5p had such regulatory functions on alveolar type II (AT-II) cells. To accomplish this, miR-20a-5p-overexpressed and miR-20a-5p-inhibited adenoviral vectors were constructed and transfected into cultured AT-II cells that were isolated from rat foetal lungs of 19 days' gestation. Transfection efficiency was confirmed by observing the fluorescence of green fluorescent protein (GFP) carried by the vi...
Source: J Cell Mol Med - September 6, 2019 Category: Molecular Biology Authors: Gong Y, Xu W, Chen Y, Liu Y, Yang Y, Wang B, Lu Z, Lin HC, Zhou X, Zhou X Tags: J Cell Mol Med Source Type: research
Identification of prognosis-related alternative splicing events in kidney renal clear cell carcinoma.
This study provided an optimized survival-predicting model and promising data resources for future in-depth studies on AS mechanisms in KIRC. PMID: 31489763 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 5, 2019 Category: Molecular Biology Authors: Zuo Y, Zhang L, Tang W, Tang W Tags: J Cell Mol Med Source Type: research
Exosomes from miR-20b-3p-overexpressing stromal cells ameliorate calcium oxalate deposition in rat kidney.
In conclusion, ADSC-derived miR-20b-3p-enriched exosomes protected against kidney stones by suppressing autophagy and inflammatory responses. PMID: 31489770 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 5, 2019 Category: Molecular Biology Authors: Shi J, Duan J, Gong H, Pang Y, Wang L, Yan Y Tags: J Cell Mol Med Source Type: research
Hypomethylation-activated cancer-testis gene SPANXC promotes cell metastasis in lung adenocarcinoma.
Abstract Many studies have shown that there were similarity between tumorigenesis and gametogenesis. Our previous work found that cancer-testis (CT) genes could serve as a novel source of candidate of cancer. Here, by analysing The Cancer Genome Atlas (TCGA) database, we characterized a CT gene, SPANXC, which is expressed only in testis. The SPANXC was reactivated in lung adenocarcinoma (LUAD) tissues. And the expression of SPANXC was associated with prognosis of LUAD. We also found that the activation of SPANXC was due to the promoter hypomethylation of SPANXC. Moreover, SPANXC could modulate cell metastasis both...
Source: J Cell Mol Med - September 4, 2019 Category: Molecular Biology Authors: Wang X, Ju S, Chen Y, Qian Q, Yan C, Chen S, Chang Y, Xu Y, Ma Z, Zhang C, Qin N, Gu Y, Wang C, Zhang E, Hu Z Tags: J Cell Mol Med Source Type: research
Long non-coding RNA SNHG16 promotes proliferation and inhibits apoptosis of diffuse large B-cell lymphoma cells by targeting miR-497-5p/PIM1 axis.
Abstract The aberrant expression and dysfunction of long non-coding RNAs (lncRNAs) have been identified as critical factors governing the initiation and progression of different human cancers, including diffuse large B-cell lymphoma (DLBCL). LncRNA small nucleolar RNA host gene 16 (SNHG16) has been recognized as a tumour-promoting factor in various types of cancer. However, the biological role of SNHG16 and its underlying mechanism are still unknown in DLBCL. Here we disclosed that SNHG16 was overexpressed in DLBCL tissues and the derived cell lines. SNHG16 knockdown significantly suppressed cell proliferation and...
Source: J Cell Mol Med - September 4, 2019 Category: Molecular Biology Authors: Zhu Q, Li Y, Guo Y, Hu L, Xiao Z, Liu X, Wang J, Xu Q, Tong X Tags: J Cell Mol Med Source Type: research
Senescence-induced immunophenotype, gene expression and electrophysiology changes in human amniocytes.
Abstract The aim of the study was to evidence replicative senescence-induced changes in human amniocytes via flow cytometry, quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) and automated/manual patch-clamp. Both cryopreserved and senescent amniocytes cultured in BIO-AMF-2 medium featured high percentages of pluripotency cell surface antigens SSEA-1, SSEA-4, TRA1-60, TRA1-81 (assessed by flow cytometry) and expression of pluripotency markers Oct4 (Pou5f1) and Nanog (by qRT-PCR). We demonstrated in senescent vs cryopreserved amniocytes decreases in mesenchymal stem cell surface markers. Senesc...
Source: J Cell Mol Med - September 3, 2019 Category: Molecular Biology Authors: Airini R, Iordache F, Alexandru D, Savu L, Epureanu FB, Mihailescu D, Amuzescu B, Maniu H Tags: J Cell Mol Med Source Type: research
Applications of sequencing technology in clinical microbial infection.
Abstract Infectious diseases are a type of disease caused by pathogenic microorganisms. Although the discovery of antibiotics changed the treatment of infectious diseases and reduced the mortality of bacterial infections, resistant bacterial strains have emerged. Anti-infective therapy based on aetiological evidence is the gold standard for clinical treatment, but the time lag and low positive culture rate of traditional methods of pathogen diagnosis leads to relative difficulty in obtaining the evidence of pathogens. Compared with traditional methods of pathogenic diagnosis, next-generation and third-generation s...
Source: J Cell Mol Med - September 2, 2019 Category: Molecular Biology Authors: Yu X, Jiang W, Shi Y, Ye H, Lin J Tags: J Cell Mol Med Source Type: research
Protective role of melatonin in early-stage and end-stage liver cirrhosis.
Abstract The liver is composed of hepatocytes, cholangiocytes, Kupffer cells, sinusoidal endothelial cells, hepatic stellate cells (HSCs) and dendritic cells; all these functional and interstitial cells contribute to the synthesis and secretion functions of liver tissue. However, various hepatotoxic factors including infection, chemicals, high-fat diet consumption, surgical procedures and genetic mutations, as well as biliary tract diseases such as sclerosing cholangitis and bile duct ligation, ultimately progress into liver cirrhosis after activation of fibrogenesis. Melatonin (MT), a special hormone isolated fro...
Source: J Cell Mol Med - September 2, 2019 Category: Molecular Biology Authors: Hu C, Zhao L, Tao J, Li L Tags: J Cell Mol Med Source Type: research
Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up-regulation of PPAR γ signalling.
In this study, we investigated how ERβ played a role in Achilles tendon healing. Achilles tendon injury model was established to analyse how ERβ affected on healing process in vivo. Cell proliferation assay, Western blots, qRT-PCR and immunocytochemistry were performed to investigate the effect of ERβ on TDSCs. Here, we showed that ERβ deletion in mice resulted in inferior gross appearance, histological scores and, most importantly, increased accumulation of adipocytes during the early tendon healing which involved activation of peroxisome proliferator-activated receptor γ (PPARγ) signalling...
Source: J Cell Mol Med - September 2, 2019 Category: Molecular Biology Authors: Bian X, Liu T, Zhou M, He G, Ma Y, Shi Y, Wang Y, Tang H, Kang X, Yang M, Gustafsson JÅ, Fan X, Tang K Tags: J Cell Mol Med Source Type: research
Prognostic power of a lipid metabolism gene panel for diffuse gliomas.
Abstract Lipid metabolism reprogramming plays important role in cell growth, proliferation, angiogenesis and invasion in cancers. However, the diverse lipid metabolism programmes and prognostic value during glioma progression remain unclear. Here, the lipid metabolism-related genes were profiled using RNA sequencing data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) database. Gene ontology (GO) and gene set enrichment analysis (GSEA) found that glioblastoma (GBM) mainly exhibited enrichment of glycosphingolipid metabolic progress, whereas lower grade gliomas (LGGs) showed enrichment of...
Source: J Cell Mol Med - September 1, 2019 Category: Molecular Biology Authors: Wu F, Zhao Z, Chai RC, Liu YQ, Li GZ, Jiang HY, Jiang T Tags: J Cell Mol Med Source Type: research
Functions and mechanisms of lysine crotonylation.
Abstract Lysine crotonylation is a newly discovered post-translational modification, which is structurally and functionally different from the widely studied lysine acetylation. Recent advances in the identification and quantification of lysine crotonylation by mass spectrometry have revealed that non-histone proteins are frequently crotonylated, implicating it in many biological processes through the regulation of chromatin remodelling, metabolism, cell cycle and cellular organization. In this review, we summarize the writers, erasers and readers of lysine crotonylation, and their physiological functions, includi...
Source: J Cell Mol Med - September 1, 2019 Category: Molecular Biology Authors: Wan J, Liu H, Chu J, Zhang H Tags: J Cell Mol Med Source Type: research
Myoferlin, a multifunctional protein in normal cells, has novel and key roles in various cancers.
Abstract Myoferlin, a protein of the ferlin family, has seven C2 domains and exhibits activity in some cells, including myoblasts and endothelial cells. Recently, myoferlin was identified as a promising target and biomarker in non-small-cell lung cancer, breast cancer, pancreatic adenocarcinoma, hepatocellular carcinoma, colon cancer, melanoma, oropharyngeal squamous cell carcinoma, head and neck squamous cell carcinoma, clear cell renal cell carcinoma and endometrioid carcinoma. This evidence indicated that myoferlin was involved in the proliferation, invasion and migration of tumour cells, the mechanism of which...
Source: J Cell Mol Med - September 1, 2019 Category: Molecular Biology Authors: Zhu W, Zhou B, Zhao C, Ba Z, Xu H, Yan X, Liu W, Zhu B, Wang L, Ren C Tags: J Cell Mol Med Source Type: research
Novel reciprocal interaction of lncRNA HOTAIR and miR-214-3p contribute to the solamargine-inhibited PDPK1 gene expression in human lung cancer.
Abstract Solamargine (SM) has been shown to have anti-cancer properties. However, the underlying mechanism involved remains undetermined. We showed that SM inhibited the growth of non-small cell lung cancer (NSCLC) cells, which was enhanced in cells with silencing of long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR), while it overcame by overexpression of HOTAIR. In addition, SM increased the expression of miR-214-3p and inhibited 3-phosphoinositide-dependent protein kinase-1 (PDPK1) gene expression, which was strengthened by miR-214-3p mimics. Intriguingly, HOTAIR could directly bind to miR-214-3...
Source: J Cell Mol Med - September 1, 2019 Category: Molecular Biology Authors: Tang Q, Zheng F, Liu Z, Wu J, Chai X, He C, Li L, Hann SS Tags: J Cell Mol Med Source Type: research
Dent disease: A window into calcium and phosphate transport.
Abstract This review examines calcium and phosphate transport in the kidney through the lens of the rare X-linked genetic disorder Dent disease. Dent disease type 1 (DD1) is caused by mutations in the CLCN5 gene encoding ClC-5, a Cl- /H+ antiporter localized to early endosomes of the proximal tubule (PT). Phenotypic features commonly include low molecular weight proteinuria (LMWP), hypercalciuria, focal global sclerosis and chronic kidney disease; calcium nephrolithiasis, nephrocalcinosis and hypophosphatemic rickets are less commonly observed. Although it is not surprising that abnormal endosomal function and rec...
Source: J Cell Mol Med - August 31, 2019 Category: Molecular Biology Authors: Anglani F, Gianesello L, Beara-Lasic L, Lieske J Tags: J Cell Mol Med Source Type: research
The bifunctional SDF-1-AnxA5 fusion protein protects cardiac function after myocardial infarction.
In this study, we constructed a fusion protein consisting of SDF-1 and AnxA5 domains. The receptor competition assay revealed that SDF-1-AnxA5 had high binding affinity to SDF-1 receptor CXCR4. The treatment of SDF-1-AnxA5 could significantly promote phosphorylation of AKT and ERK and induce chemotactic response, angiogenesis and cell survival in vitro. The binding membrane assay and immunofluorescence revealed that AnxA5 domain had the ability to specifically recognize and bind to cells injured by hypoxia. Furthermore, SDF-1-AnxA5 administered via peripheral vein could accumulate at the infarcted myocardium in vivo. The t...
Source: J Cell Mol Med - August 30, 2019 Category: Molecular Biology Authors: Huang FY, Xia TL, Li JL, Li CM, Zhao ZG, Lei WH, Chen L, Liao YB, Xiao D, Peng Y, Wang YB, Liu XJ, Chen M Tags: J Cell Mol Med Source Type: research
The cannabinoid receptor 1 is involved in renal fibrosis during chronic allograft dysfunction: Proof of concept.
dil;ois H Abstract Chronic allograft dysfunction (CAD), defined as the replacement of functional renal tissue by extracellular matrix proteins, remains the first cause of graft loss. The aim of our study was to explore the potential role of the cannabinoid receptor 1 (CB1) during CAD. We retrospectively quantified CB1 expression and correlated it with renal fibrosis in 26 kidney-transplanted patients who underwent serial routine kidney biopsies. Whereas CB1 expression was low in normal kidney grafts, it was highly expressed during CAD, especially in tubular cells. CB1 expression significantly increased early on af...
Source: J Cell Mol Med - August 30, 2019 Category: Molecular Biology Authors: Dao M, Lecru L, Vandermeersch S, Ferreira M, Ferlicot S, Posseme K, Dürrbach A, Hermeziu B, Mussini C, Chatziantoniou C, François H Tags: J Cell Mol Med Source Type: research
LncRNA TNK2-AS1 regulated ox-LDL-stimulated HASMC proliferation and migration via modulating VEGFA and FGF1 expression by sponging miR-150-5p.
This study determined the expression of lncRNA TNK2 antisense RNA 1 (TNK2-AS1) in oxidized low-density lipoprotein (ox-LDL)-stimulated human aortic smooth muscle cells (HASMCs) and examined the mechanistic role of TNK2-AS1 in the proliferation and migration of HASMCs. Our results demonstrated that ox-LDL promoted HASMC proliferation and migration, and the enhanced proliferation and migration in ox-LDL-treated HASMCs were accompanied by the up-regulation of TNK2-AS1. In vitro functional studies showed that TNK2-AS1 knockdown suppressed cell proliferation and migration of ox-LDL-stimulated HASMCs, while TNK2-AS1 overexpressi...
Source: J Cell Mol Med - August 29, 2019 Category: Molecular Biology Authors: Cai T, Cui X, Zhang K, Zhang A, Liu B, Mu JJ Tags: J Cell Mol Med Source Type: research
Long non-coding RNA cardiac hypertrophy-associated regulator governs cardiac hypertrophy via regulating miR-20b and the downstream PTEN/AKT pathway.
Abstract Pathological cardiac hypertrophy (CH) is a key factor leading to heart failure and ultimately sudden death. Long non-coding RNAs (lncRNAs) are emerging as a new player in gene regulation relevant to a wide spectrum of human disease including cardiac disorders. Here, we characterize the role of a specific lncRNA named cardiac hypertrophy-associated regulator (CHAR) in CH and delineate the underlying signalling pathway. CHAR was found markedly down-regulated in both in vivo mouse model of cardiac hypertrophy induced by pressure overload and in vitro cellular model of cardiomyocyte hypertrophy induced by ang...
Source: J Cell Mol Med - August 29, 2019 Category: Molecular Biology Authors: Zhang M, Jiang Y, Guo X, Zhang B, Wu J, Sun J, Liang H, Shan H, Zhang Y, Liu J, Wang Y, Wang L, Zhang R, Yang B, Xu C Tags: J Cell Mol Med Source Type: research
TGFBIp mediates lymphatic sprouting in corneal lymphangiogenesis.
Abstract Corneal lymphangiogenesis plays a key role in diverse pathological conditions of the eye. Here, we demonstrate that a versatile extracellular matrix protein, transforming growth factor-β induced protein (TGFBIp), promotes lymphatic sprouting in corneal lymphangiogenesis. TGFBIp is highly up-regulated in inflamed mouse corneas. Immunolocalization of TGFBIp is detected in infiltrating macrophages in inflamed mouse corneas. Subconjunctival injection of liposomal clodronate can significantly reduce macrophage infiltration in inflamed mouse cornea, and decrease the expression of TGFBIp and areas of cornea...
Source: J Cell Mol Med - August 28, 2019 Category: Molecular Biology Authors: Lin T, Zhang X, Lu Y, Gong L Tags: J Cell Mol Med Source Type: research
Long non-coding RNA LINC01225 promotes proliferation, invasion and migration of gastric cancer via Wnt/ β-catenin signalling pathway.
In conclusion, LINC01225 promotes the progression of GC through Wnt/β-catenin signalling pathway, and it may serve as a potential target or strategy for diagnosis or treatment of GC. PMID: 31460694 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - August 28, 2019 Category: Molecular Biology Authors: Xu Y, Zhang G, Zou C, Qi W, Gong Z, Zhang G, Ma G, Zhang W, Jiang P Tags: J Cell Mol Med Source Type: research
DYRK1A inhibition suppresses STAT3/EGFR/Met signalling and sensitizes EGFR wild-type NSCLC cells to AZD9291.
Abstract DYRK1A is considered a potential cancer therapeutic target, but the role of DYRK1A in NSCLC oncogenesis and treatment requires further investigation. In our study, high DYRK1A expression was observed in tumour samples from patients with lung cancer compared with normal lung tissues, and the high levels of DYRK1A were related to a reduced survival time in patients with lung cancer. Meanwhile, the DYRK1A inhibitor harmine could suppress the proliferation of NSCLC cells compared to that of the control. As DYRK1A suppression might be effective in treating NSCLC, we next explored the possible specific molecula...
Source: J Cell Mol Med - August 27, 2019 Category: Molecular Biology Authors: Li YL, Ding K, Hu X, Wu LW, Zhou DM, Rao MJ, Lin NM, Zhang C Tags: J Cell Mol Med Source Type: research