2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus
This study was aimed to explore the role of glycolysis inhibition by 2-DG on regulating immune responses of OLP-derived T cells. We observed that lactic dehydrogenase A (LDHA) expression was elevated in OLP lesions and local T cells. 2-DG inhibited the expression of LDHA, p-mTOR, Hif1α and PLD2 in T cells; meanwhile, it decreased proliferation and increased apoptosis of T cells. T cells treated by 2-DG showed lower LDHA expression and elevated apoptosis, resulting in a reduced apoptotic population of keratinocytes that were co-cultured with them, which was related to the decreased levels of IFN-γ in co-culture ...
Source: J Cell Mol Med - October 21, 2021 Category: Molecular Biology Authors: Fang Wang Jing Zhang Gang Zhou Source Type: research

Contributions of NFKB1 -94insertion/deletion ATTG polymorphism to the susceptibility of gastrointestinal cancers: A meta-analysis
J Cell Mol Med. 2021 Oct 21. doi: 10.1111/jcmm.17004. Online ahead of print.ABSTRACTNuclear factor-kappa B1 (NF-κB1), a pleiotropic transcription factor, functions as a critical contributor to tumorigenesis. Growing numbers of case-control studies were carried out to analyse the potential contribution of NF-κB1 gene variants to gastrointestinal cancer risk, yet remains conflicting conclusions. Therefore, we conducted this most up-to-date meta-analysis to evaluate the relationship between NF-κB1 gene insertion (I)/deletion (D) polymorphism, namely -94ins/delATTG or rs28362491, and the susceptibility to gas...
Source: J Cell Mol Med - October 21, 2021 Category: Molecular Biology Authors: Hanqiang Wu Jianrong Liang Source Type: research

2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus
This study was aimed to explore the role of glycolysis inhibition by 2-DG on regulating immune responses of OLP-derived T cells. We observed that lactic dehydrogenase A (LDHA) expression was elevated in OLP lesions and local T cells. 2-DG inhibited the expression of LDHA, p-mTOR, Hif1α and PLD2 in T cells; meanwhile, it decreased proliferation and increased apoptosis of T cells. T cells treated by 2-DG showed lower LDHA expression and elevated apoptosis, resulting in a reduced apoptotic population of keratinocytes that were co-cultured with them, which was related to the decreased levels of IFN-γ in co-culture ...
Source: J Cell Mol Med - October 21, 2021 Category: Molecular Biology Authors: Fang Wang Jing Zhang Gang Zhou Source Type: research

Contributions of NFKB1 -94insertion/deletion ATTG polymorphism to the susceptibility of gastrointestinal cancers: A meta-analysis
J Cell Mol Med. 2021 Oct 21. doi: 10.1111/jcmm.17004. Online ahead of print.ABSTRACTNuclear factor-kappa B1 (NF-κB1), a pleiotropic transcription factor, functions as a critical contributor to tumorigenesis. Growing numbers of case-control studies were carried out to analyse the potential contribution of NF-κB1 gene variants to gastrointestinal cancer risk, yet remains conflicting conclusions. Therefore, we conducted this most up-to-date meta-analysis to evaluate the relationship between NF-κB1 gene insertion (I)/deletion (D) polymorphism, namely -94ins/delATTG or rs28362491, and the susceptibility to gas...
Source: J Cell Mol Med - October 21, 2021 Category: Molecular Biology Authors: Hanqiang Wu Jianrong Liang Source Type: research

CircGFRA1 facilitates the malignant progression of HER-2-positive breast cancer via acting as a sponge of miR-1228 and enhancing AIFM2 expression
J Cell Mol Med. 2021 Oct 20. doi: 10.1111/jcmm.16963. Online ahead of print.ABSTRACTCircRNAs (circular RNA) are reported to regulate onset and progress multiple cancers. Nonetheless, the function along with the underlying mechanisms of circRNAs in HER-2-positive breast cancer (BC) remains unclear. CircRNA microarrays were performed to elucidate expression profiles of HER-2-positive BC cells. circRNA levels were quantified using qRT-PCR assay. Various in vitro along with in vivo assays were employed to further explore the effects of circGFRA1 in the progress of HER-2-positive BC and interactions of circGFRA1, miR-1228 and A...
Source: J Cell Mol Med - October 20, 2021 Category: Molecular Biology Authors: Meiheban Bazhabayi Xingsheng Qiu Xing Li Anli Yang Wei Wen Xiaoli Zhang Xiangsheng Xiao Rongfang He Peng Liu Source Type: research

Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression
In this study, second-generation high-throughput sequencing revealed a reduction in miR-99a-3p expression in patients with SLE; however, the specific mechanism underlying this phenomenon remains unclear. After transfection with an miR-99a-3p agomir, the proliferation of Ball-1 cells decreased and the levels of their apoptosis increased. The opposite effects were observed in cells transfected with the miR-99a-3p antagomir. Luciferase reporter assay indicated that miR-99a-3p directly targeted EIF4EBP1. Rescue experiments confirmed the proposed interaction between miR-99a-3p and EIF4EBP1. In vitro, in vivo and clinical invest...
Source: J Cell Mol Med - October 20, 2021 Category: Molecular Biology Authors: Meng Yang Binbin Yang Danqi Deng Source Type: research

CircGFRA1 facilitates the malignant progression of HER-2-positive breast cancer via acting as a sponge of miR-1228 and enhancing AIFM2 expression
J Cell Mol Med. 2021 Oct 20. doi: 10.1111/jcmm.16963. Online ahead of print.ABSTRACTCircRNAs (circular RNA) are reported to regulate onset and progress multiple cancers. Nonetheless, the function along with the underlying mechanisms of circRNAs in HER-2-positive breast cancer (BC) remains unclear. CircRNA microarrays were performed to elucidate expression profiles of HER-2-positive BC cells. circRNA levels were quantified using qRT-PCR assay. Various in vitro along with in vivo assays were employed to further explore the effects of circGFRA1 in the progress of HER-2-positive BC and interactions of circGFRA1, miR-1228 and A...
Source: J Cell Mol Med - October 20, 2021 Category: Molecular Biology Authors: Meiheban Bazhabayi Xingsheng Qiu Xing Li Anli Yang Wei Wen Xiaoli Zhang Xiangsheng Xiao Rongfang He Peng Liu Source Type: research

Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression
In this study, second-generation high-throughput sequencing revealed a reduction in miR-99a-3p expression in patients with SLE; however, the specific mechanism underlying this phenomenon remains unclear. After transfection with an miR-99a-3p agomir, the proliferation of Ball-1 cells decreased and the levels of their apoptosis increased. The opposite effects were observed in cells transfected with the miR-99a-3p antagomir. Luciferase reporter assay indicated that miR-99a-3p directly targeted EIF4EBP1. Rescue experiments confirmed the proposed interaction between miR-99a-3p and EIF4EBP1. In vitro, in vivo and clinical invest...
Source: J Cell Mol Med - October 20, 2021 Category: Molecular Biology Authors: Meng Yang Binbin Yang Danqi Deng Source Type: research

Integrative computational immunogenomic profiling of cortisol-secreting adrenocortical carcinoma
In this study, we characterized the TME of ACC patients and compared the immunogenomic profiles of nonCS-ACC and CS-ACC tumours to assess the impact of differentially expressed genes (DEGs) by utilizing The Cancer Genome Atlas (TCGA) database. Immunogenomic comparison (CS- vs. nonCS-ACC tumour TMEs) demonstrated an immunosuppressive expression profile with a direct impact on patient survival. We identified several primary prognostic indicators and potential targets within ACC tumour immune landscape. Differentially expressed immune genes with prognostic significance provide additional insight into the understanding of pote...
Source: J Cell Mol Med - October 19, 2021 Category: Molecular Biology Authors: Jordan J Baechle David N Hanna Konjeti R Sekhar Jeffrey C Rathmell W Kimryn Rathmell Naira Baregamian Source Type: research

Dexmedetomidine alleviates hepatic ischaemia-reperfusion injury via the PI3K/AKT/Nrf2-NLRP3 pathway
This study investigated protective mechanism of Dex in hepatic I/R injury. The human hepatocyte line L02 received hypoxia/reoxygenation (H/R) treatment to stimulate cell model of hepatic I/R. The levels of pyroptosis proteins and inflammatory factors were detected. Functional rescue experiments were performed to confirm the effects of miR-494 and JUND on hepatic I/R injury. The levels of JUND, PI3K/p-PI3K, AKT/p-AKT, Nrf2, and NLRP3 activation were detected. The rat model of hepatic I/R injury was established to confirm the effect of Dex in vivo. Dex reduced pyroptosis and inflammation in H/R cells. Dex increased miR-494 e...
Source: J Cell Mol Med - October 19, 2021 Category: Molecular Biology Authors: Yan Wu Gaolin Qiu Hainie Zhang Leilei Zhu Gao Cheng Yiqiao Wang Yuanhai Li Weiwei Wu Source Type: research

Integrative computational immunogenomic profiling of cortisol-secreting adrenocortical carcinoma
In this study, we characterized the TME of ACC patients and compared the immunogenomic profiles of nonCS-ACC and CS-ACC tumours to assess the impact of differentially expressed genes (DEGs) by utilizing The Cancer Genome Atlas (TCGA) database. Immunogenomic comparison (CS- vs. nonCS-ACC tumour TMEs) demonstrated an immunosuppressive expression profile with a direct impact on patient survival. We identified several primary prognostic indicators and potential targets within ACC tumour immune landscape. Differentially expressed immune genes with prognostic significance provide additional insight into the understanding of pote...
Source: J Cell Mol Med - October 19, 2021 Category: Molecular Biology Authors: Jordan J Baechle David N Hanna Konjeti R Sekhar Jeffrey C Rathmell W Kimryn Rathmell Naira Baregamian Source Type: research

Dexmedetomidine alleviates hepatic ischaemia-reperfusion injury via the PI3K/AKT/Nrf2-NLRP3 pathway
This study investigated protective mechanism of Dex in hepatic I/R injury. The human hepatocyte line L02 received hypoxia/reoxygenation (H/R) treatment to stimulate cell model of hepatic I/R. The levels of pyroptosis proteins and inflammatory factors were detected. Functional rescue experiments were performed to confirm the effects of miR-494 and JUND on hepatic I/R injury. The levels of JUND, PI3K/p-PI3K, AKT/p-AKT, Nrf2, and NLRP3 activation were detected. The rat model of hepatic I/R injury was established to confirm the effect of Dex in vivo. Dex reduced pyroptosis and inflammation in H/R cells. Dex increased miR-494 e...
Source: J Cell Mol Med - October 19, 2021 Category: Molecular Biology Authors: Yan Wu Gaolin Qiu Hainie Zhang Leilei Zhu Gao Cheng Yiqiao Wang Yuanhai Li Weiwei Wu Source Type: research

Comprehensive landscape and interference of clonal haematopoiesis mutations for liquid biopsy: A Chinese pan-cancer cohort
J Cell Mol Med. 2021 Oct 17. doi: 10.1111/jcmm.16966. Online ahead of print.ABSTRACTTumour-derived DNA found in the plasma of cancer patients provides the probability to detect somatic mutations from circulating cell-free DNA (cfDNA) in plasma samples. However, clonal hematopoiesis (CH) mutations affect the accuracy of liquid biopsy for cancer diagnosis and treatment. Here, we integrated landscape of CH mutations in 11,725 pan-cancer patients of Chinese and explored effects of CH on liquid biopsies in real-world. We first identified 5933 CHs based on panel sequencing of matched DNA of white blood cell and cfDNA on 301 gene...
Source: J Cell Mol Med - October 18, 2021 Category: Molecular Biology Authors: Enwu Xu Kai Su Yang Zhou Longlong Gong Yiwen Xuan Ming Liao Jiawang Cao Yaqian Li Yujiao Lu Yi Zhao Fengxia Chen Source Type: research

Colon cancer cells secreted CXCL11 via RBP-J κ to facilitated tumour-associated macrophage-induced cancer metastasis
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16989. Online ahead of print.ABSTRACTMetastasis is the main cause of colon cancer-related deaths. RBP-Jκ is involved in colon cancer development, but its function in colon cancer metastasis is still unclear. Tumour-associated macrophages are the main cell components in tumour microenvironments. Here, we aimed to determine the function of RBP-Jκ in colon cancer metastasis and its underlying mechanisms for modulating interactions between colon cancer cell and tumour-associated macrophages. Through bioinformation analysis, we found that RBP-Jκ was overexpressed ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Mengjie Liu Xiao Fu Lili Jiang Jiequn Ma Xiaoqiang Zheng Shuhong Wang Hui Guo Tao Tian Kejun Nan Wenjuan Wang Source Type: research

ERK5 signalling pathway is a novel target of sorafenib: Implication in EGF biology
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16990. Online ahead of print.ABSTRACTSorafenib is a multikinase inhibitor widely used in cancer therapy with an antitumour effect related to biological processes as proliferation, migration or invasion, among others. Initially designed as a Raf inhibitor, Sorafenib was later shown to also block key molecules in tumour progression such as VEGFR and PDGFR. In addition, sorafenib has been connected with key signalling pathways in cancer such as EGFR/EGF. However, no definitive clue about the molecular mechanism linking sorafenib and EGF signalling pathway has been established so ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Marta Ortega-Muelas Olga Roche Diego M Fern ández-Aroca Jos é A Encinar David Albandea-Rodr íguez Elena Arconada-Luque Raquel Pascual-Serra Ismael Mu ñoz Isabel S ánchez-Pérez Borja Belandia Mar ía J Ruiz-Hidalgo Ricardo S ánchez-Prieto Source Type: research

Colon cancer cells secreted CXCL11 via RBP-J κ to facilitated tumour-associated macrophage-induced cancer metastasis
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16989. Online ahead of print.ABSTRACTMetastasis is the main cause of colon cancer-related deaths. RBP-Jκ is involved in colon cancer development, but its function in colon cancer metastasis is still unclear. Tumour-associated macrophages are the main cell components in tumour microenvironments. Here, we aimed to determine the function of RBP-Jκ in colon cancer metastasis and its underlying mechanisms for modulating interactions between colon cancer cell and tumour-associated macrophages. Through bioinformation analysis, we found that RBP-Jκ was overexpressed ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Mengjie Liu Xiao Fu Lili Jiang Jiequn Ma Xiaoqiang Zheng Shuhong Wang Hui Guo Tao Tian Kejun Nan Wenjuan Wang Source Type: research

ERK5 signalling pathway is a novel target of sorafenib: Implication in EGF biology
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16990. Online ahead of print.ABSTRACTSorafenib is a multikinase inhibitor widely used in cancer therapy with an antitumour effect related to biological processes as proliferation, migration or invasion, among others. Initially designed as a Raf inhibitor, Sorafenib was later shown to also block key molecules in tumour progression such as VEGFR and PDGFR. In addition, sorafenib has been connected with key signalling pathways in cancer such as EGFR/EGF. However, no definitive clue about the molecular mechanism linking sorafenib and EGF signalling pathway has been established so ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Marta Ortega-Muelas Olga Roche Diego M Fern ández-Aroca Jos é A Encinar David Albandea-Rodr íguez Elena Arconada-Luque Raquel Pascual-Serra Ismael Mu ñoz Isabel S ánchez-Pérez Borja Belandia Mar ía J Ruiz-Hidalgo Ricardo S ánchez-Prieto Source Type: research

Colon cancer cells secreted CXCL11 via RBP-J κ to facilitated tumour-associated macrophage-induced cancer metastasis
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16989. Online ahead of print.ABSTRACTMetastasis is the main cause of colon cancer-related deaths. RBP-Jκ is involved in colon cancer development, but its function in colon cancer metastasis is still unclear. Tumour-associated macrophages are the main cell components in tumour microenvironments. Here, we aimed to determine the function of RBP-Jκ in colon cancer metastasis and its underlying mechanisms for modulating interactions between colon cancer cell and tumour-associated macrophages. Through bioinformation analysis, we found that RBP-Jκ was overexpressed ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Mengjie Liu Xiao Fu Lili Jiang Jiequn Ma Xiaoqiang Zheng Shuhong Wang Hui Guo Tao Tian Kejun Nan Wenjuan Wang Source Type: research

ERK5 signalling pathway is a novel target of sorafenib: Implication in EGF biology
J Cell Mol Med. 2021 Oct 16. doi: 10.1111/jcmm.16990. Online ahead of print.ABSTRACTSorafenib is a multikinase inhibitor widely used in cancer therapy with an antitumour effect related to biological processes as proliferation, migration or invasion, among others. Initially designed as a Raf inhibitor, Sorafenib was later shown to also block key molecules in tumour progression such as VEGFR and PDGFR. In addition, sorafenib has been connected with key signalling pathways in cancer such as EGFR/EGF. However, no definitive clue about the molecular mechanism linking sorafenib and EGF signalling pathway has been established so ...
Source: J Cell Mol Med - October 16, 2021 Category: Molecular Biology Authors: Marta Ortega-Muelas Olga Roche Diego M Fern ández-Aroca Jos é A Encinar David Albandea-Rodr íguez Elena Arconada-Luque Raquel Pascual-Serra Ismael Mu ñoz Isabel S ánchez-Pérez Borja Belandia Mar ía J Ruiz-Hidalgo Ricardo S ánchez-Prieto Source Type: research

Preclinical assessment of thrombin-preconditioned human Wharton's jelly-derived mesenchymal stem cells for neonatal hypoxic-ischaemic brain injury
This study was conducted to evaluate the tissue distribution and potential toxicity of thrombin-preconditioned human Wharton's jelly-derived mesenchymal stem cells (th-hWJMSCs) in animal models before the initiation of clinical trials. We investigated the biodistribution, tumorigenicity and general toxicity of th-hWJMSCs. MSCs were administered the maximum feasible dose (1 × 105 cells/10 µL/head) once, or at lower doses into the cerebral ventricle. To support the clinical use of th-hWJMSCs for treating brain injury, preclinical safety studies were conducted in newborn Sprague-Dawley rats and BALB/c nude mice. I...
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Jung-Ho Noh Ji-Seong Jeong Sang-Jin Park Kyung Jin Jung Byoung-Seok Lee Woo-Jin Kim Ji-Seok Han Min-Kyung Cho Dong Kyung Sung So Yoon Ahn Yun Sil Chang Hwa-Young Son Eun Ju Jeong Source Type: research

LINC00184 plays an oncogenic role in non-small cell lung cancer via regulation of the miR-524-5p/HMGB2 axis
J Cell Mol Med. 2021 Oct 15. doi: 10.1111/jcmm.16247. Online ahead of print.ABSTRACTNon-small cell lung cancer (NSCLC) is the most common type of lung cancer. We aimed to investigate the role of LINC00184 in NSCLC. Migration, proliferation and invasion of NSCLC cells were analysed using the wound healing assay, cell counting kit-8 assay and transwell assay, respectively. Apoptosis and cell cycle were assessed using flow cytometry. Online bioinformatics tools were utilized to predict downstream microRNAs (miRNA) or genes related to LINC00184 expression. The RNA pull-down experiment and luciferase reporter assay were perform...
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Wuming Wang Lin Li Long Zhao Source Type: research

Prolactin and endocrine therapy resistance in breast cancer: The next potential hope for breast cancer treatment
J Cell Mol Med. 2021 Oct 15. doi: 10.1111/jcmm.16946. Online ahead of print.ABSTRACTBreast cancer, a hormone-dependent tumour, generally includes four molecular subtypes (luminal A, luminal B, HER2 enriched and triple-negative) based on oestrogen receptor, progesterone receptor and human epidermal growth factor receptor-2. Multiple hormones in the body regulate the development of breast cancer. Endocrine therapy is one of the primary treatments for hormone-receptor-positive breast cancer, but endocrine resistance is the primary clinical cause of treatment failure. Prolactin (PRL) is a protein hormone secreted by the pituit...
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Yuan Li Xiangyi Kong Lixue Xuan Zhongzhao Wang Yen-Hua Huang Source Type: research

Mucin 20 modulates proteasome capacity through c-Met signalling to increase carfilzomib sensitivity in mantle cell lymphoma
This study aimed to explore the mechanism of PI resistance in MCL and identify new targets for this patient subgroup. Carfilzomib-resistant (CR) MCL cell lines and primary samples were used for both in vitro and in vivo experiments to identify gene expression and explore their related signalling pathways. We first identified mucin 20 (MUC20) suppression in carfilzomib-resistant MCL models. MUC20 overexpression sensitized cells to carfilzomib in vitro and in vivo. MUC20 expression was inversely related to activation of c-Met and the downstream p44/42 MAPK pathway. c-Met activation with hepatocyte growth factor (HGF) induced...
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Xiaobin Wang Fazal Shirazi Wei Yan Xiaoyu Liu Hua Wang Robert Z Orlowski Huihan Wang Source Type: research

Bisphosphonate-enoxacin inhibit osteoclast formation and function by abrogating RANKL-induced JNK signalling pathways during osteoporosis treatment
J Cell Mol Med. 2021 Oct 15. doi: 10.1111/jcmm.16949. Online ahead of print.ABSTRACTOsteoporosis is an age-related disease characterized by low mineral density, compromised bone strength and increased risk of fragility fracture. Most agents for treating osteoporosis focus primarily on anti-resorption by inhibiting osteoclast activity. Bisphosphonate (BP) is a potent anti-resorptive agent that has been used clinically for decades and is proven to be effective. However, BP has a variety of side effects and is far from being an ideal anti-osteoporosis agent. BP selectively binds to calcium crystals, which are subsequently tak...
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Qiang Xu Ping Zhan Xiaofeng Li Fengbo Mo Huaen Xu Yuan Liu Qi Lai Bin Zhang Min Dai Xuqiang Liu Source Type: research

Spontaneous arising of a lymphoblastoid B-cell line harbouring a pre-leukemic DNMT3A mutation in acute myeloid leukaemia cell culture
J Cell Mol Med. 2021 Oct 14. doi: 10.1111/jcmm.16992. Online ahead of print.NO ABSTRACTPMID:34651440 | DOI:10.1111/jcmm.16992 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 15, 2021 Category: Molecular Biology Authors: Michal Hayun Moran Szwarcwort Dina Rosenberg Dvora Sahar Yishai Ofran Source Type: research

Matrix metalloproteinase-9 inhibition or deletion attenuates portal hypertension in rodents
In conclusion, MMP-9 inhibition or deletion ameliorated the severity of cirrhosis, portal hypertension, and associated derangements. MMP-9 may be targeted in the treatment of liver cirrhosis.PMID:34647412 | DOI:10.1111/jcmm.16940 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 14, 2021 Category: Molecular Biology Authors: Hui-Chun Huang Hsin-Ling Ho Ching-Chih Chang Chiao-Lin Chuang Chon Kit Pun Fa-Yauh Lee Yi-Hsiang Huang Ming-Chih Hou Shao-Jung Hsu Source Type: research

m6A regulator-mediated RNA methylation modification patterns and immune microenvironment infiltration characterization in severe asthma
In conclusion, our findings suggested that m6A modification plays a key role in severe asthma, and may be able to guide the future strategy of immunotherapy.PMID:34647423 | DOI:10.1111/jcmm.16961 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 14, 2021 Category: Molecular Biology Authors: Deyang Sun Huan Yang Liming Fan Fenglin Shen Zhen Wang Source Type: research

Matrix metalloproteinase-9 inhibition or deletion attenuates portal hypertension in rodents
In conclusion, MMP-9 inhibition or deletion ameliorated the severity of cirrhosis, portal hypertension, and associated derangements. MMP-9 may be targeted in the treatment of liver cirrhosis.PMID:34647412 | DOI:10.1111/jcmm.16940 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 14, 2021 Category: Molecular Biology Authors: Hui-Chun Huang Hsin-Ling Ho Ching-Chih Chang Chiao-Lin Chuang Chon Kit Pun Fa-Yauh Lee Yi-Hsiang Huang Ming-Chih Hou Shao-Jung Hsu Source Type: research

m6A regulator-mediated RNA methylation modification patterns and immune microenvironment infiltration characterization in severe asthma
In conclusion, our findings suggested that m6A modification plays a key role in severe asthma, and may be able to guide the future strategy of immunotherapy.PMID:34647423 | DOI:10.1111/jcmm.16961 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 14, 2021 Category: Molecular Biology Authors: Deyang Sun Huan Yang Liming Fan Fenglin Shen Zhen Wang Source Type: research

MicroRNA-146b-5p promotes atrial fibrosis in atrial fibrillation by repressing TIMP4
This study was to determine whether miR-146b-5p could regulate the gene expression of TIMP4 and the contribution of miRNA to atrial fibrosis in AF. Collagen synthesis was observed after miR-146b-5p transfection in human induced pluripotent stem cell-derived atrial cardiomyocytes (hiPSC-aCMs)-fibroblast co-culture cellular model in vitro. Furthermore, a myocardial infarction (MI) mouse model was used to confirm the protective effect of miR-146b-5p downregulation on atrial fibrosis. The expression level of miR-146b-5p was upregulated, while the expression level of TIMP4 was downregulated in the fibrotic atrium of canine with...
Source: J Cell Mol Med - October 13, 2021 Category: Molecular Biology Authors: Qing Ye Quan Liu Xiaolong Ma Shuyun Bai Pengfei Chen Yichen Zhao Chen Bai Yang Liu Kemin Liu Meng Xin Caiwu Zeng Cheng Zhao Yan Yao Yue Ma Jiangang Wang Source Type: research

Endogenous tRNA-derived small RNA (tRF3-Thr-AGT) inhibits ZBP1/NLRP3 pathway-mediated cell pyroptosis to attenuate acute pancreatitis (AP)
This study, for the first time, identified the role and potential underlying mechanisms by which tRF3-Thr-AGT regulated AP pathogenesis.PMID:34643045 | DOI:10.1111/jcmm.16972 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 13, 2021 Category: Molecular Biology Authors: Boshi Sun Zhuomiaoyu Chen Qiang Chi Yifan Zhang Bo Gao Source Type: research

MicroRNA-146b-5p promotes atrial fibrosis in atrial fibrillation by repressing TIMP4
This study was to determine whether miR-146b-5p could regulate the gene expression of TIMP4 and the contribution of miRNA to atrial fibrosis in AF. Collagen synthesis was observed after miR-146b-5p transfection in human induced pluripotent stem cell-derived atrial cardiomyocytes (hiPSC-aCMs)-fibroblast co-culture cellular model in vitro. Furthermore, a myocardial infarction (MI) mouse model was used to confirm the protective effect of miR-146b-5p downregulation on atrial fibrosis. The expression level of miR-146b-5p was upregulated, while the expression level of TIMP4 was downregulated in the fibrotic atrium of canine with...
Source: J Cell Mol Med - October 13, 2021 Category: Molecular Biology Authors: Qing Ye Quan Liu Xiaolong Ma Shuyun Bai Pengfei Chen Yichen Zhao Chen Bai Yang Liu Kemin Liu Meng Xin Caiwu Zeng Cheng Zhao Yan Yao Yue Ma Jiangang Wang Source Type: research

Endogenous tRNA-derived small RNA (tRF3-Thr-AGT) inhibits ZBP1/NLRP3 pathway-mediated cell pyroptosis to attenuate acute pancreatitis (AP)
This study, for the first time, identified the role and potential underlying mechanisms by which tRF3-Thr-AGT regulated AP pathogenesis.PMID:34643045 | DOI:10.1111/jcmm.16972 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 13, 2021 Category: Molecular Biology Authors: Boshi Sun Zhuomiaoyu Chen Qiang Chi Yifan Zhang Bo Gao Source Type: research

miR-363-3p induces EMT via the Wnt/ β-catenin pathway in glioma cells by targeting CELF2
In conclusion, miR-363-3p induced the EMT, which resulted in increased migration and invasion and reduced apoptosis in glioma cell lines, via the Wnt/β-catenin pathway by targeting CELF2.PMID:34636136 | DOI:10.1111/jcmm.16970 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 12, 2021 Category: Molecular Biology Authors: Bo Fan Bolun Su Guoqiang Song Xin Liu Zhongjie Yan Shuai Wang Fuguang Hu Jiankai Yang Source Type: research

NUDT15 polymorphism and NT5C2 and PRPS1 mutations influence thiopurine sensitivity in acute lymphoblastic leukaemia cells
J Cell Mol Med. 2021 Oct 12. doi: 10.1111/jcmm.16981. Online ahead of print.ABSTRACTIn chemotherapy for childhood acute lymphoblastic leukaemia (ALL), maintenance therapy consisting of oral daily mercaptopurine and weekly methotrexate is important. NUDT15 variant genotype is reportedly highly associated with severe myelosuppression during maintenance therapy, particularly in Asian and Hispanic populations. It has also been demonstrated that acquired somatic mutations of the NT5C2 and PRPS1 genes, which are involved in thiopurine metabolism, are detectable in a portion of relapsed childhood ALL. To directly confirm the sign...
Source: J Cell Mol Med - October 12, 2021 Category: Molecular Biology Authors: Shinpei Somazu Yoichi Tanaka Minori Tamai Atsushi Watanabe Keiko Kagami Masako Abe Daisuke Harama Tamao Shinohara Koshi Akahane Kumiko Goi Kanji Sugita Takaya Moriyama Jun Yang Hiroaki Goto Masayoshi Minegishi Shotaro Iwamoto Junko Takita Takeshi Inukai Source Type: research

miRNA-34b/c regulates mucus secretion in RSV-infected airway epithelial cells by targeting FGFR1
This study identified that miR-34b/miR-34c was involved in c-Jun-regulated MUC5AC production by targeting FGFR1 in RSV-infected airway epithelial cells. These results provide some useful insights into the molecular mechanisms of mucus hypersecretion which may also bring new potential strategies to improve mucus hypersecretion in RSV disease.PMID:34636482 | DOI:10.1111/jcmm.16988 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 12, 2021 Category: Molecular Biology Authors: Wenkai Li Xizi Du Yu Yang Lin Yuan Ming Yang Ling Qin Leyuan Wang Kai Zhou Yang Xiang Xiangping Qu Huijun Liu Xiaoqun Qin Gelei Xiao Chi Liu Source Type: research

Identification of pyroptosis-related lncRNAs for constructing a prognostic model and their correlation with immune infiltration in breast cancer
In this study, a profile of 8 differentially expressed lncRNAs was screened in the TCGA database and used to construct a prognostic model. The BC patients were divided into high- and low-risk groups dependent on the median cutoff of the risk score in the model. Interestingly, the risk model significantly distinguished the clinical characteristics of BC patients between high- and low-risk groups. Then, the risk score of the model was identified to be an excellent independent prognostic factor. Notably, the GO, KEGG, GSEA and ssGSEA analyses revealed the different immune statuses between the high- and low-risk groups. Partic...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Wenchang Lv Yufang Tan Chongru Zhao Yichen Wang Min Wu Yiping Wu Yuping Ren Qi Zhang Source Type: research

Mafenide derivatives inhibit neuroinflammation in Alzheimer's disease by regulating pyroptosis
In this study, sulfonamide was used as the parent nucleus structure to synthesize sulfa-4 and sulfa-20. Screening of drug activity in the pyroptosis model of BV2 and iBMDM cell lines revealed the efficacy of five compounds were superior to mafenide, which exerted a better inhibitory effect on the occurrence of pyroptosis. For in vivo assay, Sulfa-4 and Sulfa-22 were intervened in the neuroinflammation APP/PS1 mice. As a result, the administration of Sulfa-4 and Sulfa-22 could significantly inhibit the activation of microglia, decrease the expression of inflammatory factors in the central nervous system and simultaneously s...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Chenyang Han Qiaohong Hu Anqi Yu Qingcai Jiao Yi Yang Source Type: research

Conquering the cytokine storm in COVID-19-induced ARDS using placenta-derived decidua stromal cells
J Cell Mol Med. 2021 Oct 10. doi: 10.1111/jcmm.16986. Online ahead of print.ABSTRACTAcute respiratory distress syndrome (ARDS) is the most common cause of death in COVID-19 patients. The cytokine storm is the main driver of the severity and magnitude of ARDS. Placenta-derived decidua stromal cells (DSCs) have a stronger immunosuppressive effect than other sources of mesenchymal stromal cells. Safety and efficacy study included 10 patients with a median age of 50 (range 14-68) years with COVID-19-induced ARDS. DSCs were administered 1-2 times at a dose of 1 × 106 /kg. End points were safety and efficacy by survival, o...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Behnam Sadeghi Elham Roshandel Ali Pirsalehi Sepide Kazemi Ghazaleh Sankanian Mohammad Majidi Maryam Salimi Nasser Aghdami Hoda Sadrosadat Sarvenaz Samadi Kochaksaraei Farshid Alaeddini Olle Ringden Abbas Hajifathali Source Type: research

Comparative transcriptomic analysis of THP-1-derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG
In this study, RNA sequencing (transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived from macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb-infected macrophages and then sequenced. To assess the transcriptional differences between the expressed genes, the bioinformatics analysis was performed using a standard pipeline of quality control, reference mapping...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Wenyuan Pu Chen Zhao Junaid Wazir Zhonglan Su Mengyuan Niu Shiyu Song Lulu Wei Li Li Xia Zhang Xudong Shi Hongwei Wang Source Type: research

Identification of pyroptosis-related lncRNAs for constructing a prognostic model and their correlation with immune infiltration in breast cancer
In this study, a profile of 8 differentially expressed lncRNAs was screened in the TCGA database and used to construct a prognostic model. The BC patients were divided into high- and low-risk groups dependent on the median cutoff of the risk score in the model. Interestingly, the risk model significantly distinguished the clinical characteristics of BC patients between high- and low-risk groups. Then, the risk score of the model was identified to be an excellent independent prognostic factor. Notably, the GO, KEGG, GSEA and ssGSEA analyses revealed the different immune statuses between the high- and low-risk groups. Partic...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Wenchang Lv Yufang Tan Chongru Zhao Yichen Wang Min Wu Yiping Wu Yuping Ren Qi Zhang Source Type: research

Mafenide derivatives inhibit neuroinflammation in Alzheimer's disease by regulating pyroptosis
In this study, sulfonamide was used as the parent nucleus structure to synthesize sulfa-4 and sulfa-20. Screening of drug activity in the pyroptosis model of BV2 and iBMDM cell lines revealed the efficacy of five compounds were superior to mafenide, which exerted a better inhibitory effect on the occurrence of pyroptosis. For in vivo assay, Sulfa-4 and Sulfa-22 were intervened in the neuroinflammation APP/PS1 mice. As a result, the administration of Sulfa-4 and Sulfa-22 could significantly inhibit the activation of microglia, decrease the expression of inflammatory factors in the central nervous system and simultaneously s...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Chenyang Han Qiaohong Hu Anqi Yu Qingcai Jiao Yi Yang Source Type: research

Conquering the cytokine storm in COVID-19-induced ARDS using placenta-derived decidua stromal cells
J Cell Mol Med. 2021 Oct 10. doi: 10.1111/jcmm.16986. Online ahead of print.ABSTRACTAcute respiratory distress syndrome (ARDS) is the most common cause of death in COVID-19 patients. The cytokine storm is the main driver of the severity and magnitude of ARDS. Placenta-derived decidua stromal cells (DSCs) have a stronger immunosuppressive effect than other sources of mesenchymal stromal cells. Safety and efficacy study included 10 patients with a median age of 50 (range 14-68) years with COVID-19-induced ARDS. DSCs were administered 1-2 times at a dose of 1 × 106 /kg. End points were safety and efficacy by survival, o...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Behnam Sadeghi Elham Roshandel Ali Pirsalehi Sepide Kazemi Ghazaleh Sankanian Mohammad Majidi Maryam Salimi Nasser Aghdami Hoda Sadrosadat Sarvenaz Samadi Kochaksaraei Farshid Alaeddini Olle Ringden Abbas Hajifathali Source Type: research

Comparative transcriptomic analysis of THP-1-derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG
In this study, RNA sequencing (transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived from macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb-infected macrophages and then sequenced. To assess the transcriptional differences between the expressed genes, the bioinformatics analysis was performed using a standard pipeline of quality control, reference mapping...
Source: J Cell Mol Med - October 11, 2021 Category: Molecular Biology Authors: Wenyuan Pu Chen Zhao Junaid Wazir Zhonglan Su Mengyuan Niu Shiyu Song Lulu Wei Li Li Xia Zhang Xudong Shi Hongwei Wang Source Type: research

Role of HRTPT in kidney proximal epithelial cell regeneration: Integrative differential expression and pathway analyses using microarray and scRNA-seq
In conclusion, the gene expression analysis identified a unique gene set for HRTPT cells and narrowed the co-expressed gene set compared with other human renal-derived cell lines expressing CD133, which may provide deeper understanding in their role as progenitor/stem cells that participate in renal repair.PMID:34626063 | DOI:10.1111/jcmm.16976 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: Swojani Shrestha Sonalika Singhal Matthew Kalonick Rachel Guyer Alexis Volkert Seema Somji Scott H Garrett Donald A Sens Sandeep K Singhal Source Type: research

Regulation of autophagy protects against liver injury in liver surgery-induced ischaemia/reperfusion
J Cell Mol Med. 2021 Oct 8. doi: 10.1111/jcmm.16943. Online ahead of print.ABSTRACTTransient ischaemia and reperfusion in liver tissue induce hepatic ischaemia/reperfusion (I/R) tissue injury and a profound inflammatory response in vivo. Hepatic I/R can be classified into warm I/R and cold I/R and is characterized by three main types of cell death, apoptosis, necrosis and autophagy, in rodents or patients following I/R. Warm I/R is observed in patients or animal models undergoing liver resection, haemorrhagic shock, trauma, cardiac arrest or hepatic sinusoidal obstruction syndrome when vascular occlusion inhibits normal bl...
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: Chenxia Hu Lingfei Zhao Fen Zhang Lanjuan Li Source Type: research

Epithelial-Mesenchymal Transition in colorectal cancer: Annexin A2 is caught in the crosshairs
J Cell Mol Med. 2021 Oct 8. doi: 10.1111/jcmm.16962. Online ahead of print.NO ABSTRACTPMID:34626069 | DOI:10.1111/jcmm.16962 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: M R Rocha Jose Andres Morgado-Diaz Source Type: research

DHFR silence alleviated the development of liver fibrosis by affecting the crosstalk between hepatic stellate cells and macrophages
In this study, mRNAs with significant differences were explored using exosomal RNA-sequencing of activated Lx-2 cells and normal RNA-sequencing of DHFR loss-of-function Lx-2 cell models. Cell functional experiments in both Lx-2 cells and macrophages animal model experiments were performed. The results basically confirmed exosomes secreted from activated HSCs could promote M1 polarization of macrophages further. Exosome harbouring DHFR played an important role in this process. DHFR silence in HSCs could decrease Lx-2 activation and M1 polarization of M0 macrophages and then alleviate the development of liver fibrosis both i...
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: Yu Peng Zedong Li Sheng Chen Jun Zhou Source Type: research

GPX7 Facilitates BMSCs Osteoblastogenesis via ER Stress and mTOR Pathway
J Cell Mol Med. 2021 Oct 9. doi: 10.1111/jcmm.16974. Online ahead of print.ABSTRACTEmerging evidence indicates extensive oxidative stress is a consequence of obesity which impairs bone formation. Glutathione peroxidase 7 (GPX7) is a conserved endoplasmic reticulum (ER) retention protein, lacking of which causes accumulation of reactive oxygen species (ROS) and promotes adipogenesis. Since the imbalance between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cell (BMSC) leads to severe bone diseases such as osteoporosis, it is critical to investigate the potential protective role of Gpx7 in osteoge...
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: Xuchen Hu Boer Li Fanzi Wu Xiaoyu Liu Mengyu Liu Chenglin Wang Yu Shi Ling Ye Source Type: research

Role of HRTPT in kidney proximal epithelial cell regeneration: Integrative differential expression and pathway analyses using microarray and scRNA-seq
In conclusion, the gene expression analysis identified a unique gene set for HRTPT cells and narrowed the co-expressed gene set compared with other human renal-derived cell lines expressing CD133, which may provide deeper understanding in their role as progenitor/stem cells that participate in renal repair.PMID:34626063 | DOI:10.1111/jcmm.16976 (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2021 Category: Molecular Biology Authors: Swojani Shrestha Sonalika Singhal Matthew Kalonick Rachel Guyer Alexis Volkert Seema Somji Scott H Garrett Donald A Sens Sandeep K Singhal Source Type: research