Platelet inhibitory effects of the Phase 3 anticancer and normal tissue cytoprotective agent, RRx-001.
Abstract The platelet inhibitory effects of the Phase 3 anticancer agent and nitric oxide (NO) donor, RRx-001, (1-bromoacetyl-3,3-dinitroazetidine) were examined ex vivo and compared with the diazeniumdiolate NO donor, diethylenetriamine NONOate (DETA-NONOate), which spontaneously releases nitric oxide in aqueous solution. In the absence of red blood cells and in a dose-dependent manner, DETA-NONOate strongly inhibited platelet aggregation induced by several stimuli (ADP, epinephrine and collagen) whereas RRx-001 only slightly inhibited platelet aggregation under the same conditions in a dose-dependent manner...
Source: J Cell Mol Med - July 16, 2018 Category: Molecular Biology Authors: Oronsky B, Oronsky N, Cabrales P Tags: J Cell Mol Med Source Type: research
Dual effects of baicalin on osteoclast differentiation and bone resorption.
In this study, we found that baicalin exerted a two-way regulatory effect on OC in a concentration-dependent manner in vitro and in vivo. In detail, baicalin at a low concentration (below 1 μmol/L) enhanced OC differentiation and bone resorption, but baicalin at a high concentration (above 2 μmol/L) exhibited inhibitory effects on OC. We demonstrated that baicalin at low concentrations enhanced the mitogen-activated protein kinase (MAPK) (ERK) signalling pathway and activated c-Fos and NFATc1 expression, and thus enhanced gene expression, OC differentiation and bone resorption. However, baicalin a...
Source: J Cell Mol Med - July 16, 2018 Category: Molecular Biology Authors: Lu X, He W, Yang W, Li J, Han W, Liu Q, Zhang T, Jiang J, Qin A, Qian Y Tags: J Cell Mol Med Source Type: research
Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway.
Abstract Tumour growth depends on a continual supply of the nutrients and oxygen, which are offered by tumour angiogenesis. Our previous study showed that dipalmitoylphosphatidic acid (DPPA), a bioactive phospholipid, inhibits the growth of triple-negative breast cancer cells. However, its direct effect on angiogenesis remains unknown. Our work showed that DPPA significantly suppressed vascular growth in the chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models. Meanwhile, tumour angiogenesis and tumour growth were inhibited by DPPA in the tumour tissues of an experimental breast cancer mo...
Source: J Cell Mol Med - July 16, 2018 Category: Molecular Biology Authors: Chen J, Zhou Z, Yao Y, Dai J, Zhou D, Wang L, Zhang QQ Tags: J Cell Mol Med Source Type: research
Long non-coding RNA NNT-AS1 sponges miR-424/E2F1 to promote the tumorigenesis and cell cycle progression of gastric cancer.
In conclusion, our study indicates that NNT-AS1 sponges miR-424/E2F1 to facilitate GC tumorigenesis and cycle progress, revealing the oncogenic role of NNT-AS1 for GC. PMID: 30006956 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 14, 2018 Category: Molecular Biology Authors: Chen B, Zhao Q, Guan L, Lv H, Bie L, Huang J, Chen XB Tags: J Cell Mol Med Source Type: research
Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells.
Abstract Endocrine disorders have become more and more frequently diagnosed in humans and animals. In horses, equine metabolic syndrome (EMS) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation and usually by pathological obesity. Due to an increased inflammatory response in the adipose tissue, cytophysiological properties of adipose derived stem cells (ASC) have been impaired, which strongly limits their therapeutic potential. Excessive accumulation of reactive oxygen species, mitochondria deterioration and accelerated ageing of those cells affect their multipotency and restric...
Source: J Cell Mol Med - July 12, 2018 Category: Molecular Biology Authors: Marycz K, Kornicka K, Irwin-Houston JM, Weiss C Tags: J Cell Mol Med Source Type: research
Aberrant O-glycosylation contributes to tumorigenesis in human colorectal cancer.
Abstract Aberrant O-glycosylation is frequently observed in colorectal cancer (CRC) patients, but it is unclear if it contributes intrinsically to tumorigenesis. Here, we investigated the biological consequences of aberrant O-glycosylation in CRC. We first detected the expression profile of Tn antigen in a serial of human CRC tissues and then explored the genetic and biosynthetic mechanisms. Moreover, we used a human CRC cell line (LS174T), which express Tn antigen, to assess whether aberrant O-glycosylation can directly promote oncogenic properties. It showed that Tn antigen was detected in around 86% human prima...
Source: J Cell Mol Med - July 12, 2018 Category: Molecular Biology Authors: Jiang Y, Liu Z, Xu F, Dong X, Cheng Y, Hu Y, Gao T, Liu J, Yang L, Jia X, Qian H, Wen T, An G Tags: J Cell Mol Med Source Type: research
Heterogeneity of lipidomic profiles among lung cancer subtypes of patients.
Abstract Lung cancer is a leading cause of cancer-related deaths with an increasing incidence and poor prognoses. To further understand the regulatory mechanisms of lipidomic profiles in lung cancer subtypes, we measure the profiles of plasma lipidome between health and patients with lung cancer or among patients with squamous cell carcinomas, adenocarcinoma or small cell lung cancer and to correct lipidomic and genomic profiles of lipid-associated enzymes and proteins by integrating the data of large-scale genome screening. Our studies demonstrated that circulating levels of PS and lysoPS significantly increased,...
Source: J Cell Mol Med - July 12, 2018 Category: Molecular Biology Authors: Lv J, Gao D, Zhang Y, Wu D, Shen L, Wang X Tags: J Cell Mol Med Source Type: research
Tubular epithelial C1orf54 mediates protection and recovery from acute kidney injury.
Abstract Acute kidney injury (AKI) incidence among hospitalized patients is increasing steadily. Despite progress in prevention strategies and support measures, AKI remains correlated with high mortality, particularly among ICU patients, and no effective AKI therapy exists. Here, we investigated the function in kidney ischaemia-reperfusion injury (IRI) of C1orf54, a newly identified protein encoded by an open reading frame on chromosome 1. C1orf54 expression was high in kidney and low in heart, liver, spleen, lung and skeletal muscle in healthy mice, and in the kidney, C1orf54 was expressed in tubular epithelial c...
Source: J Cell Mol Med - July 12, 2018 Category: Molecular Biology Authors: Xie H, Wang Y, Zhang H, Fan Q, Dai D, Zhuang L, Tao R, Chen Q, Shen W, Lu L, Ding X, Zhang R, Yan X Tags: J Cell Mol Med Source Type: research
ASK1 inhibitor treatment suppresses p38/JNK signalling with reduced kidney inflammation and fibrosis in rat crescentic glomerulonephritis.
In conclusion, GS-444217 treatment inhibited p38/JNK activation and development of renal injury in rat NTN. ASK1 inhibitors may have therapeutic potential in rapidly progressive glomerulonephritis. PMID: 29998485 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Amos LA, Ma FY, Tesch GH, Liles JT, Breckenridge DG, Nikolic-Paterson DJ, Han Y Tags: J Cell Mol Med Source Type: research
Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey.
Abstract Acute liver failure (ALF) is associated with high mortality, and a poor understanding of the underlying pathophysiology has resulted in a lack of effective treatments so far. Here, using an amatoxin-induced rhesus monkey model of ALF, we panoramically revealed the cellular and molecular events that lead to the development of ALF. The challenged monkeys with toxins underwent a typical course of ALF including severe hepatic injury, systemic inflammation and eventual death. Adaptive immune was not noticeably disturbed throughout the progress of ALF. A systematic examination of serum factors and cytokines rev...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Guo G, Zhu Y, Wu Z, Ji H, Lu X, Zhou Y, Li Y, Cao X, Lu Y, Talbot P, Liao J, Shi Y, Bu H Tags: J Cell Mol Med Source Type: research
The involvement of Toll-like receptor 9 in the pathogenesis of erosive autoimmune arthritis.
, Steiner G Abstract Endogenous nucleic acids and their receptors may be involved in the initiation of systemic autoimmune diseases including rheumatoid arthritis (RA). As the role of the DNA sensing Toll-like receptor (TLR) 9 in RA is unclear, we aimed to investigate its involvement in the pathogenesis of autoimmune arthritis using three different experimental models of RA. The data obtained revealed involvement of TLR9 in the T cell-dependent phase of inflammatory arthritis. In rats with pristane-induced arthritis (PIA), TLR9 inhibition before disease onset reduced arthritis significantly and almost completely a...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Fischer A, Abdollahi-Roodsaz S, Böhm C, Niederreiter B, Meyer B, Yau ACY, Lönnblom E, Joosten LAB, Koenders M, Lehmann CHK, Dudziak D, Krönke G, Holmdahl R, Steiner G Tags: J Cell Mol Med Source Type: research
Heat-shock transcription factor 1 is critically involved in the ischaemia-induced cardiac hypertrophy via JAK2/STAT3 pathway.
Abstract Cardiac hypertrophy after myocardial infarction (MI) is an independent risk factor for heart failure. Regression of cardiac hypertrophy has emerged as a promising strategy in the treatment of MI patients. Here, we have been suggested that heat-shock transcription factor 1 (HSF1) is a novel repressor of ischaemia-induced cardiac hypertrophy. Ligation of left anterior descending coronary was used to produce MI in HSF1-deficient heterozygote (KO), HSF1 transgenic (TG) mice and their wild-type (WT) littermates, respectively. Neonatal rat cardiomyocytes (NRCMs) were treated by hypoxia to mimic MI in vitro. The...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Yuan L, Qiu L, Ye Y, Wu J, Wang S, Wang X, Zhou N, Zou Y Tags: J Cell Mol Med Source Type: research
Kallistatin attenuates endothelial senescence by modulating Let-7g-mediated miR-34a-SIRT1-eNOS pathway.
This study is aimed to investigate the role and mechanism of kallistatin in endothelial senescence. Kallistatin inhibited H2 O2 -induced senescence in human endothelial cells, as indicated by reduced senescence-associated-β-galactosidase activity, p16INK4a and plasminogen activator inhibitor-1 expression, and elevated telomerase activity. Kallistatin blocked H2 O2 -induced superoxide formation, NADPH oxidase levels and VCAM-1, ICAM-1, IL-6 and miR-34a synthesis. Kallistatin reversed H2 O2 -mediated inhibition of endothelial nitric oxide synthase (eNOS), SIRT1, catalase and superoxide dismutase (SOD)-2 expression, and ...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Guo Y, Chao L, Chao J Tags: J Cell Mol Med Source Type: research
Aldolase A as a prognostic factor and mediator of progression via inducing epithelial-mesenchymal transition in gastric cancer.
This study aimed to explore the clinicopathological significance and potential role of glycolytic enzyme aldolase A (ALDOA) in the carcinogenesis and progression of gastric cancer (GC). ALDOA was screened from three paired liver metastasis tissues and primary GC tissues and further explored with clinical samples and in vitro studies. The ALDOA protein level significantly correlated with a larger tumor diameter (P = .004), advanced T stage (P
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Jiang Z, Wang X, Li J, Yang H, Lin X Tags: J Cell Mol Med Source Type: research
Gypenosides improve diabetic cardiomyopathy by inhibiting ROS-mediated NLRP3 inflammasome activation.
Abstract NLRP3 inflammasome activation plays an important role in diabetic cardiomyopathy (DCM), which may relate to excessive production of reactive oxygen species (ROS). Gypenosides (Gps), the major ingredients of Gynostemma pentaphylla (Thunb.) Makino, have exerted the properties of anti-hyperglycaemia and anti-inflammation, but whether Gps improve myocardial damage and the mechanism remains unclear. Here, we found that high glucose (HG) induced myocardial damage by activating the NLRP3 inflammasome and then promoting IL-1β and IL-18 secretion in H9C2 cells and NRVMs. Meanwhile, HG elevated the production ...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Zhang H, Chen X, Zong B, Yuan H, Wang Z, Wei Y, Wang X, Liu G, Zhang J, Li S, Cheng G, Wang Y, Ma Y Tags: J Cell Mol Med Source Type: research
Rho GTPases are involved in S1P-enhanced glomerular endothelial cells activation with anti-myeloperoxidase antibody positive IgG.
In this study, we demonstrated that Rho guanosine triphosphatases (GTPases) signalling pathways, RhoA and Rac1 in particular, were implicated in MPO-ANCA-positive IgG-mediated GEnCs activation enhanced by pathophysiological concentration of S1P in AAV. These results provide mechanistic insights into vascular barrier dysfunction in AAV, which may facilitate the development of effective therapies. PMID: 29993181 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Sun XJ, Chen M, Zhao MH Tags: J Cell Mol Med Source Type: research
Distinct roles for I κB kinases alpha and beta in regulating pulmonary endothelial angiogenic function during late lung development.
In this study, we sought to delineate the specific roles for the NFκB activating kinases, IKKα and IKKβ, in promoting developmental pulmonary angiogenesis. Microarray analysis of primary pulmonary endothelial cells (PECs) after silencing IKKα or IKKβ demonstrated that the 2 kinases regulate unique panels of genes, with few shared targets. Although silencing IKKα induced mild impairments in angiogenic function, silencing IKKβ induced more severe angiogenic defects and decreased vascular cell adhesion molecule expression, an IKKβ regulated target essential for both PEC adhesion and...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Iosef C, Liu M, Ying L, Rao SP, Concepcion KR, Chan WK, Oman A, Alvira CM Tags: J Cell Mol Med Source Type: research
Bone marrow mesenchymal stem cells reduce ureteral stricture formation in a rat model via the paracrine effect of extracellular vesicles.
Abstract With no effective therapy to prevent or treat ureteral stricture (US), a multifactorial fibrotic disease after iatrogenic injury of the ureter, the need for new therapies is urgent. Mesenchymal stem cells (MSCs) have been widely studied for treating tissue defects and excessive fibrosis, and recent studies established that one of the main therapeutic vectors of MSCs is comprised in their secretome and represented by extracellular vesicles (EVs). Thus, we have determined to explore the specific role of MSCs-derived EVs (MSC-EVs) treatment in a pre-clinical model of US. The results firstly showed that eithe...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Luo J, Zhao S, Wang J, Luo L, Li E, Zhu Z, Liu Y, Kang R, Zhao Z Tags: J Cell Mol Med Source Type: research
Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide-Gonearrestide.
In this study, a novel scorpion venom-derived peptide named Gonearrestide was identified in an in-house constructed scorpion venom library through a combination of high-throughput NGS transcriptome and MS/MS proteome platform. In total, 238 novel peptides were discovered from two scorpion species; and 22 peptides were selected for further study after a battery of functional prediction analysis. Following a series of bioinformatics analysis alongside with in vitro biological functional screenings, Gonearrestide was found to be a highly potent anticancer peptide which acts on a broad spectrum of human cancer cells while...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Li B, Lyu P, Xi X, Ge L, Mahadevappa R, Shaw C, Kwok HF Tags: J Cell Mol Med Source Type: research
Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway.
Abstract Osteosarcoma becomes the second leading cause of cancer death in the younger population. Current outcomes of chemotherapy on osteosarcoma were unsatisfactory to date, demanding development of effective therapies. Tea is a commonly used beverage beneficial to human health. As a major component of tea, theabrownin has been reported to possess anti-cancer activity. To evaluate its anti-osteosarcoma effect, we established a xenograft model of zebrafish and employed U2OS cells for in vivo and in vitro assays. The animal data showed that TB significantly inhibited the tumour growth with stronger effec...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Jin W, Zhou L, Yan B, Yan L, Liu F, Tong P, Yu W, Dong X, Xie L, Zhang J, Xu Y, Li C, Yuan Q, Shan L, Efferth T Tags: J Cell Mol Med Source Type: research
Levamisole suppresses adipogenesis of aplastic anaemia-derived bone marrow mesenchymal stem cells through ZFP36L1-PPARGC1B axis.
Abstract Aplastic anaemia (AA) is a life-threatening hematopoietic disorder characterized by hypoplasia and pancytopenia with increasing fat cells in the bone marrow (BM). The BM-derived mesenchymal stem cells (MSCs) from AA are more susceptible to be induced into adipogenic differentiation compared with that from control, which may be causatively associated with the fatty BM and defective hematopoiesis of AA. Here in this study, we first demonstrated that levamisole displayed a significant suppressive effect on the in vitro adipogenic differentiation of AA BM-MSCs. Mechanistic investigation revealed that lev...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Liu LL, Liu L, Liu HH, Ren SS, Dou CY, Cheng PP, Wang CL, Wang LN, Chen XL, Zhang H, Chen MT Tags: J Cell Mol Med Source Type: research
Modelling cadmium-induced cardiotoxicity using human pluripotent stem cell-derived cardiomyocytes.
Abstract Cadmium, a highly ubiquitous toxic heavy metal, has been widely recognized as an environmental and industrial pollutant, which confers serious threats to human health. The molecular mechanisms of the cadmium-induced cardiotoxicity (CIC) have not been studied in human cardiomyocytes at the cellular level. Here we showed that human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) can recapitulate the CIC at the cellular level. The cadmium-treated hPSC-CMs exhibited cellular phenotype including reduced cell viability, increased apoptosis, cardiac sarcomeric disorganization, elevated reactive oxygen sp...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Shen J, Wang X, Zhou D, Li T, Tang L, Gong T, Su J, Liang P Tags: J Cell Mol Med Source Type: research
Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1 α.
This study aims to investigate the mechanism of calycosin related to tight junctions (TJs) and HIF-1α both in FITC-induced mice allergic contact dermatitis and in IL-1β stimulated HaCaT keratinocytes. Th2 cytokines (IL-4, IL-5 and IL-13) were detected by ELISA. The epithelial TJ proteins (occludin, CLDN1 and ZO-1), initiative key cytokines (TSLP and IL-33) and HIF-1α were assessed by Western blot, real-time PCR, immunohistochemistry or immunofluorescence. Herein, we have demonstrated that allergic inflammation and the Th2 cytokines in ACD mice were reduced significantly by calycosin treatment. Meanwhile, c...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Jia Z, Wang X, Wang X, Wei P, Li L, Wu P, Hong M Tags: J Cell Mol Med Source Type: research
The oncogenic potentials and diagnostic significance of long non-coding RNA LINC00310 in breast cancer.
Abstract Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in different physiological processes and human diseases. However, to date, the function and overall clinical significance of the vast majority of lncRNAs in breast cancer remain largely unexplored. Here, we focused on LINC00310 by interrogating the breast invasive carcinoma data set of the Cancer Genome Atlas (TCGA). The results showed that LINC00310 was increased as breast cancer progressed, and the deregulation of LINC00310 was significantly associated with patients' survival. Experiments with knockout (KO) approach by CRISPR/...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Li J, Peng W, Du L, Yang Q, Wang C, Mo YY Tags: J Cell Mol Med Source Type: research
Dynamin-related protein 1-mediated mitochondrial fission contributes to IR-783-induced apoptosis in human breast cancer cells.
In this study, we showed that IR-783 inhibits cell viability and induces mitochondrial apoptosis in human breast cancer cells. Exposure of MDA-MB-231 cells to IR-783 resulted in the loss of mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) depletion, mitochondrial permeability transition pore (mPTP) opening and cytochrome c (Cyto C) release. Furthermore, we found that IR-783 induced dynamin-related protein 1 (Drp1) translocation from the cytosol to the mitochondria, increased the expression of mitochondrial fission proteins mitochondrial fission factor (MFF) and fission-1 (Fis1), and decreased the expres...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Tang Q, Liu W, Zhang Q, Huang J, Hu C, Liu Y, Wang Q, Zhou M, Lai W, Sheng F, Li G, Zhang R Tags: J Cell Mol Med Source Type: research
Diagnostic and therapeutic value of progastrin-releasing peptide on small-cell lung cancer: A Single-Center Experience in China.
Abstract We aimed to compare the diagnostic efficiency of proGRP and NSE on SCLC and to investigate whether the change of proGRP level would predict therapeutic response. Patients who were firstly diagnosed pathologically in Nanjing Chest Hospital and measured proGRP level consecutively were enrolled in the study. ProGRP level was detected using Elecsys ProGRP Assay. Totally 75 SCLC, 234 NSCLC and 264 benign lung diseases (BLD) were enrolled. Both proGRP and NSE levels in SCLC were significantly higher than those in NSCLC and BLD, and proGRP in extensive stage SCLC was higher than which in limited stage (P &l...
Source: J Cell Mol Med - July 10, 2018 Category: Molecular Biology Authors: Wu XY, Hu YB, Li HJ, Wan B, Zhang CX, Zhang B, Hu H, Zhang Q, Lv TF, Zhan P, Song Y Tags: J Cell Mol Med Source Type: research
The expression of MMP-1 and MMP-9 is up-regulated by smooth muscle cells after their cross-talk with macrophages in high glucose conditions.
Abstract Patients with diabetes mellitus have an increased risk of myocardial infarction and coronary artery disease-related death, exhibiting highly vulnerable plaques. Many studies have highlighted the major role of macrophages (MAC) and smooth muscle cells (SMC) and the essential part of metalloproteases (MMPs) in atherosclerotic plaque vulnerability. We hypothesize that in diabetes, the interplay between MAC and SMC in high glucose conditions may modify the expression of MMPs involved in plaque vulnerability. The SMC-MAC cross-talk was achieved using trans-well chambers, where human SMC were grown at the botto...
Source: J Cell Mol Med - July 10, 2018 Category: Molecular Biology Authors: Macarie RD, Vadana M, Ciortan L, Tucureanu MM, Ciobanu A, Vinereanu D, Manduteanu I, Simionescu M, Butoi E Tags: J Cell Mol Med Source Type: research
The association between paraoxonase 1 activity and the susceptibilities of diabetes mellitus, diabetic macroangiopathy and diabetic microangiopathy.
In conclusion, paraoxonase 1 activity plays important roles in the risk of DM, diabetic macroangiopathy and microangiopathy with ethnicity differences. Further studies with large sample and well design are needed to confirm these results. PMID: 29981194 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 7, 2018 Category: Molecular Biology Authors: Wu D, Wu C, Zhong Y Tags: J Cell Mol Med Source Type: research
RNF168 facilitates oestrogen receptor ɑ transcription and drives breast cancer proliferation.
Abstract Oestrogen receptor ɑ (ERɑ) is overexpressed in two-thirds of all breast cancers and involves in development and breast cancer progression. Although ERɑ-positive breast cancer could be effective treated by endocrine therapy, the endocrine resistance is still an urgent clinical problem. Thus, further understanding of the underlying mechanisms ERɑ signalling is critical in dealing with endocrine resistance in breast cancer patients. MCF-7 and T47D breast cancer cell lines are used to carry out the molecular biological experiments. Western blot is used to assess the relative protein level of ERɑ, RNF168 ...
Source: J Cell Mol Med - July 5, 2018 Category: Molecular Biology Authors: Liu Z, Zhang J, Xu J, Yang H, Li X, Hou Y, Zhao Y, Xue M, Wang B, Yu N, Yu S, Niu G, Wu G, Li X, Wang H, Zhu J, Zhuang T Tags: J Cell Mol Med Source Type: research
HO-1 regulates the function of Treg: Association with the immune intolerance in vitiligo.
In this study, we explored the molecular and cellular alterations accounting for the impaired Treg response in vitiligo. Our results showed that the amount of Tregs was drastically reduced in peripheral blood of active vitiligo patients. Furthermore, the immunoregulatory function of Tregs was attenuated, with lower expression of CTLA4, IL-10 and TGF-β. Moreover, the expression of HO-1, a functional modulator of Tregs, was decreased in vitiligo Tregs, and the concentrations of HO-1 metabolites, including bilirubin, CoHb and iron, were correspondingly decreased in serum of vitiligo patients. In addition, we treated the ...
Source: J Cell Mol Med - July 5, 2018 Category: Molecular Biology Authors: Zhang Q, Cui T, Chang Y, Zhang W, Li S, He Y, Li B, Liu L, Wang G, Gao T, Li C, Jian Z Tags: J Cell Mol Med Source Type: research
Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms.
Abstract Alterations in the bone marrow niche induced by abnormal production of cytokines and other soluble factors have been associated with disease progression in classical BCR-ABL1 negative myeloproliferative neoplasms (MPN). Variations in circulating proteins might reflect local disease processes and plasma proteome profiling could serve to identify possible diagnostic and prognostic biomarkers. We employed a human cytokine array to screen for 105 distinct analytes in pooled plasma samples obtained from untreated young MPN patients (
Source: J Cell Mol Med - July 5, 2018 Category: Molecular Biology Authors: Mambet C, Necula L, Mihai S, Matei L, Bleotu C, Chivu-Economescu M, Stanca O, Tatic A, Berbec N, Tanase C, Diaconu CC Tags: J Cell Mol Med Source Type: research
Captopril mitigates splenomegaly and myelofibrosis in the Gata1low murine model of myelofibrosis.
Abstract Allogeneic stem cell transplantation is currently the only curative therapy for primary myelofibrosis (MF), while the JAK2 inhibitor, ruxolitinib. Has been approved only for palliation. Other therapies are desperately needed to reverse life-threatening MF. However, the cell(s) and cytokine(s) that promote MF remain unclear. Several reports have demonstrated that captopril, an inhibitor of angiotensin-converting enzyme that blocks the production of angiotensin II (Ang II), mitigates fibrosis in heart, lung, skin and kidney. Here, we show that captopril can mitigate the development of MF in the Gata1low mou...
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Corey SJ, Jha J, McCart EA, Rittase WB, George J, Mattapallil JJ, Mehta H, Ognoon M, Bylicky MA, Summers TA, Day RM Tags: J Cell Mol Med Source Type: research
Long non-coding RNA AFAP1-AS1/miR-320a/RBPJ axis regulates laryngeal carcinoma cell stemness and chemoresistance.
Abstract AFAP1-AS1 is a long non-coding RNA that is associated with tumorigenesis and poor prognosis in a variety of cancers. We have been suggested that AFAP1-AS1 increases tumorigenesis in laryngeal carcinoma specifically by enhancing stemness and chemoresistance. We assessed AFAP1-AS1 expression in human laryngeal specimens, paired adjacent normal tissues and human HEp-2 cells. Indeed, we found not only that AFAP1-AS1 was up-regulated in laryngeal carcinoma specimens and cells, but also that stemness-associated genes were overexpressed. Silencing of AFAP1-AS1 promoted HEp-2 cell chemoresistance under cisplatin ...
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Yuan Z, Xiu C, Song K, Pei R, Miao S, Mao X, Sun J, Jia S Tags: J Cell Mol Med Source Type: research
Inference of patient-specific subpathway activities reveals a functional signature associated with the prognosis of patients with breast cancer.
In this study, we aimed to infer patient-specific subpathway activities to reveal a functional signature associated with the prognosis of patients with breast cancer. We integrated pathway structure with gene expression data to construct patient-specific subpathway activity profiles using a greedy search algorithm. A four-subpathway prognostic signature was developed in the training set using a random forest supervised classification algorithm and a prognostic score model with the activity profiles. According to the signature, patients were classified into high-risk and low-risk groups with significantly different overall ...
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Han J, Liu S, Jiang Y, Xu C, Zheng B, Jiang M, Yang H, Su F, Li C, Zhang Y Tags: J Cell Mol Med Source Type: research
TWEAK/Fn14 mediates atrial-derived HL-1 myocytes hypertrophy via JAK2/STAT3 signalling pathway.
In conclusion, TWEAK/Fn14 axis mediates HL-1 atrial myocytes hypertrophy partly through activation of the JAK2/STAT3 pathway. PMID: 29971943 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Hao L, Ren M, Rong B, Xie F, Lin MJ, Zhao YC, Yue X, Han WQ, Zhong JQ Tags: J Cell Mol Med Source Type: research
Astragaloside IV modulates TGF- β1-dependent epithelial-mesenchymal transition in bleomycin-induced pulmonary fibrosis.
Astragaloside IV modulates TGF-β1-dependent epithelial-mesenchymal transition in bleomycin-induced pulmonary fibrosis. J Cell Mol Med. 2018 Jul 04;: Authors: Qian W, Cai X, Qian Q, Zhang W, Wang D Abstract Epithelial-mesenchymal transition (EMT) plays an important role in idiopathic pulmonary fibrosis (IPF). Astragaloside IV (ASV), a natural saponin from astragalus membranaceus, has shown anti-fibrotic property in bleomycin (BLM)-induced pulmonary fibrosis. The current study was undertaken to determine whether EMT was involved in the beneficial of ASV against BLM-induced pulmonary fibrosis and to...
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Qian W, Cai X, Qian Q, Zhang W, Wang D Tags: J Cell Mol Med Source Type: research
Signalling through Src family kinase isoforms is not redundant in models of thrombo-inflammatory vascular disease.
Abstract The Src family kinases (SFK) are a group of signalling molecules with important regulatory functions in inflammation and haemostasis. Leucocytes and platelets express multiple isoforms of the SFKs. Previous studies used broad-spectrum pharmacological inhibitors, or murine models deficient in multiple SFK isoforms, to demonstrate the functional consequences of deficiencies in SFK signalling. Here, we hypothesized that individual SFK operate in a non-redundant fashion in the thrombo-inflammatory recruitment of monocyte during atherosclerosis. Using in vitro adhesion assays and single SFK knockout mice ...
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Harrison MJ, Chimen M, Hussain M, Iqbal AJ, Senis YA, Nash GB, Watson SP, Rainger GE Tags: J Cell Mol Med Source Type: research
Long non-coding RNA FTH1P3 activates paclitaxel resistance in breast cancer through miR-206/ABCB1.
In this study, we investigate the physiopathologic role of lncRNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) on the paclitaxel (PTX) resistance in breast cancer. Results showed that lncRNA FTH1P3 was up-regulated in paclitaxel-resistant breast cancer tissue and cells (MCF-7/PTX and MDA-MB-231/PTX cells) compared with paclitaxel-sensitive tissue and parental cell lines (MCF-7, MDA-MB-231). Gain- and loss-of-function experiments revealed that FTH1P3 silencing decreased the 50% inhibitory concentration (IC50) value of paclitaxel and induced cell cycle arrest at G2/M phase, while FTH1P3-enhanced expression exerted the opposi...
Source: J Cell Mol Med - July 3, 2018 Category: Molecular Biology Authors: Wang R, Zhang T, Yang Z, Jiang C, Seng J Tags: J Cell Mol Med Source Type: research
CYP2E1 Rsa Ι/Pst Ι polymorphism and lung cancer susceptibility: A meta-analysis involving 10,947 subjects.
Discussion" sections of this study and the following article published in Lung Cancer: "CYP2E1 Rsa I/Pst I polymorphism is associated with lung cancer risk among Asians" by Ping Zhan, Jing Wang, Yu Zhang, Li-Xin Qiu, Su-feng Zhao, Qian Qian, Shu-Zhen Wei, Li-Ke Yu and Yong Song, Volume 69, 2010, pages 19-25. REFERENCE Shen Z-T, Wu X-H, Li B, Shen J-S, Wang Z, Li J, Zhu X-X. CYP2E1 Rsa Ι/Pst Ι polymorphism and lung cancer susceptibility: a meta-analysis involving 10,947 subjects. J Cell Mol Med. 2015;19:2136-2142. https://doi.org/10.1111/jcmm.12579. PMID: 29924497 [PubMed - in process] (Source: J Cell Mol Med)
Source: J Cell Mol Med - June 23, 2018 Category: Molecular Biology Authors: Shen ZT, Wu XH, Li B, Shen JS, Wang Z, Li J, Zhu XX Tags: J Cell Mol Med Source Type: research
Corilagin inhibits breast cancer growth via reactive oxygen species-dependent apoptosis and autophagy.
Abstract Corilagin is a component of Phyllanthus urinaria extract and has been found of possessing anti-inflammatory, anti-oxidative, and anti-tumour properties in clinic treatments. However, the underlying mechanisms in anti-cancer particularly of its induction of cell death in human breast cancer remain undefined. Our research found that corilagin-induced apoptotic and autophagic cell death depending on reactive oxygen species (ROS) in human breast cancer cell, and it occurred in human breast cancer cell (MCF-7) only comparing with normal cells. The expression of procaspase-8, procaspase-3, PARP, Bcl-2 and proca...
Source: J Cell Mol Med - June 19, 2018 Category: Molecular Biology Authors: Tong Y, Zhang G, Li Y, Xu J, Yuan J, Zhang B, Hu T, Song G Tags: J Cell Mol Med Source Type: research
Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer.
In this study, we investigated the effects of H2 inhalation on pressure ulcer and the underlying mechanisms. H2 inhalation significantly reduced wound area, 8-oxo-dG level (oxidative DNA damage) and cell apoptosis rates in skin lesions. H2 remarkably decreased ROS accumulation and enhanced antioxidant enzymes activities by up-regulating expression of Nrf2 and its downstream components in wound tissue and/or H2 O2 -treated endothelia. Meanwhile, H2 inhibited the overexpression of MCP-1, E-selectin, P-selectin and ICAM-1 in oxidant-induced endothelia and reduced inflammatory cells infiltration and proinflammatory cytokines (...
Source: J Cell Mol Med - June 19, 2018 Category: Molecular Biology Authors: Fang W, Wang G, Tang L, Su H, Chen H, Liao W, Xu J Tags: J Cell Mol Med Source Type: research
Identification of new biophysical markers for pathological ventricular remodelling in tachycardia-induced dilated cardiomyopathy.
e;s V Abstract Our aim was to identify biophysical biomarkers of ventricular remodelling in tachycardia-induced dilated cardiomyopathy (DCM). Our study includes healthy controls (N = 7) and DCM pigs (N = 10). Molecular analysis showed global myocardial metabolic abnormalities, some of them related to myocardial hibernation in failing hearts, supporting the translationality of our model to study cardiac remodelling in dilated cardiomyopathy. Histological analysis showed unorganized and agglomerated collagen accumulation in the dilated ventricles and a higher percentage of fibrosis in the right (...
Source: J Cell Mol Med - June 19, 2018 Category: Molecular Biology Authors: Benitez-Amaro A, Samouillan V, Jorge E, Dandurand J, Nasarre L, de Gonzalo-Calvo D, Bornachea O, Amoros-Figueras G, Lacabanne C, Vilades D, Leta R, Carreras F, Gallardo A, Lerma E, Cinca J, Guerra JM, Llorente-Cortés V Tags: J Cell Mol Med Source Type: research
Cardioprotection of ischaemic preconditioning is associated with inhibition of translocation of MLKL within the plasma membrane.
Čarnická S, Ravingerová T, Adameová A Abstract Necroptosis, a form of cell loss involving the RIP1-RIP3-MLKL axis, has been identified in cardiac pathologies while its inhibition is cardioprotective. We investigated whether the improvement of heart function because of ischaemic preconditioning is associated with mitigation of necroptotic signaling, and these effects were compared with a pharmacological antinecroptotic approach targeting RIP1. Langendorff-perfused rat hearts were subjected to ischaemic preconditioning with or without a RIP1 inhibitor (Nec-1s). Necroptotic signaling and the ass...
Source: J Cell Mol Med - June 19, 2018 Category: Molecular Biology Authors: Szobi A, Farkašová-Ledvényiová V, Lichý M, Muráriková M, Čarnická S, Ravingerová T, Adameová A Tags: J Cell Mol Med Source Type: research
Curcumin prevents the bile reflux-induced NF- κB-related mRNA oncogenic phenotype, in human hypopharyngeal cells.
Curcumin prevents the bile reflux-induced NF-κB-related mRNA oncogenic phenotype, in human hypopharyngeal cells. J Cell Mol Med. 2018 Jun 17;: Authors: Vageli DP, Doukas SG, Spock T, Sasaki CT Abstract The presence of bile is not an uncommon finding in acidic oesophageal and extra-oesophageal refluxate, possibly affecting the hypopharyngeal mucosa and leading to neoplastic events. We recently demonstrated that acidic bile (pH ≤ 4.0) can induce NF-κB activation and oncogenic mRNA phenotype in normal hypopharyngeal cells and generate premalignant changes in treated hypopharyngea...
Source: J Cell Mol Med - June 17, 2018 Category: Molecular Biology Authors: Vageli DP, Doukas SG, Spock T, Sasaki CT Tags: J Cell Mol Med Source Type: research
Inhibition effects of a natural inhibitor on RANKL downstream cellular signalling cascades cross-talking.
Abstract Myricitrin is a natural occurring flavonoid glycoside that possesses effects on inhibiting nitric oxide (NO) transmission and preventing inflammatory reaction. Although previous study showed the myricitrin possesses antibone loss effects via reducing the expression of IL-6 and partially suppressing reactive oxygen species (ROS) production. However, the effects of myricitrin on nuclear factor-kappaB ligand (RANKL)-stimulated osteoclastogenesis have not yet been further investigated. The current study was aimed to demonstrating the inhibitory effects of myricitrin on RANKL-stimulated osteoclastogenesis and ...
Source: J Cell Mol Med - June 17, 2018 Category: Molecular Biology Authors: Wang B, Hao D, Zhang Z, Gao W, Pan H, Xiao Y, He B, Kong L Tags: J Cell Mol Med Source Type: research
LncRNA BDNF-AS inhibits proliferation, migration, invasion and EMT in oesophageal cancer cells by targeting miR-214.
This study was aimed at exploring the effect of lncRNA BDNF-AS on cell proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT) of oesophageal cancer (EC) cells. The expression of BDNF-AS and miR-214 in tissue samples and cells was measured by qRT-PCR. The targeted relationship between BDNF-AS and miR-214 was analysed by dual-luciferase reporter assay. After cell transfection, the cell proliferation activity was assessed by MTS method, while the migrating and invading abilities were evaluated by transwell assay. LncRNA BDNF-AS was remarkably down-regulated, while miR-214 was up-regulated in EC tiss...
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Zhao H, Diao C, Wang X, Xie Y, Liu Y, Gao X, Han J, Li S Tags: J Cell Mol Med Source Type: research
Exosomes, the message transporters in vascular calcification.
Abstract Vascular calcification (VC) is caused by hydroxyapatite deposition in the intimal and medial layers of the vascular wall, leading to severe cardiovascular events in patients with hypertension, chronic kidney disease and diabetes mellitus. VC occurrences involve complicated mechanism networks, such as matrix vesicles or exosomes production, osteogenic differentiation, reduced cell viability, aging and so on. However, with present therapeutic methods targeting at VC ineffectively, novel targets for VC treatment are demanded. Exosomes are proven to participate in VC and function as initializers for mineral d...
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Zhang C, Zhang K, Huang F, Feng W, Chen J, Zhang H, Wang J, Luo P, Huang H Tags: J Cell Mol Med Source Type: research
miR-200b ameliorates myofibroblast transdifferentiation in precancerous oral submucous fibrosis through targeting ZEB2.
In this study, we investigated the impact of miR-200b on myofibroblastic differentiation activity. Arecoline is a major areca nut alkaloid and has been employed to induce the elevated myofibroblast activity in human buccal mucosal fibroblasts (BMFs). Treatment of arecoline in BMFs dose-dependently reduced gene expression of miR-200b, which corresponded with the decreased expression of miR-200b in fBMFs. The arecoline-induced myofibroblast activities were abolished by overexpression of miR-200b in BMFs, and the same results were observed in fBMFs. In addition, α-SMA was inhibited by an increase in miR-200b. We further...
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Liao YW, Yu CC, Hsieh PL, Chang YC Tags: J Cell Mol Med Source Type: research
CIP2A facilitates the G1/S cell cycle transition via B-Myb in human papillomavirus 16 oncoprotein E6-expressing cells.
In this study, we demonstrated that HPV-16E6 significantly up-regulated CIP2A mRNA and protein expression in a p53-degradation-dependent manner. Knockdown of CIP2A by siRNA inhibited viability and DNA synthesis and caused G1 cell cycle arrest of 16E6-expressing cells. Knockdown of CIP2A resulted in a significant reduction in the expression of cyclin-dependent kinase 1 (Cdk1) and Cdk2. Although CIP2A has been reported to stabilize c-Myc by inhibiting PP2A-mediated dephosphorylation of c-Myc, we have presented evidence that the regulation of Cdk1 and Cdk2 by CIP2A is dependent on transcription factor B-Myb rather than c-Myc....
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Tian Y, Chen H, Qiao L, Zhang W, Zheng J, Zhao W, Chen JJ, Zhang W Tags: J Cell Mol Med Source Type: research
Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.
In this study, the possibility to use in vitro-generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro-produced megakaryocytes. In comparison with platelets, in vitro-generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet-derived growth factors (PDGFs), transforming growth factor (TGF)-1β, vascular endothelial cell factor (VEGF)-A, epiderm...
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Baigger A, Eicke D, Yuzefovych Y, Pogozhykh D, Blasczyk R, Figueiredo C Tags: J Cell Mol Med Source Type: research