Activation of transient receptor potential vanilloid 3 channel (TRPV3) aggravated pathological cardiac hypertrophy via calcineurin/NFATc3 pathway in rats.
In conclusion, the activation of TRPV3 aggravated pathological cardiac hypertrophy through calcineurin/NFATc3 signalling pathway and correlated with the protein expression levels of calcineurin, phosphorylated CaMKII and NFATc3, revealing that TRPV3 might be a potential therapeutic target for cardiac hypertrophy. PMID: 30299584 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2018 Category: Molecular Biology Authors: Zhang Q, Qi H, Cao Y, Shi P, Song C, Ba L, Chen Y, Gao J, Li S, Li B, Sun H Tags: J Cell Mol Med Source Type: research

Adenosine deaminase inhibition suppresses progression of 4T1 murine breast cancer by adenosine receptor-dependent mechanisms.
This study highlights beneficial effects of adenosine deaminase inhibition on breast cancer development. The inhibition of adenosine deaminase activity by dCF reduced tumour size that was closely related to the decreased aggressiveness of tumour cells by adenosine receptor-dependent mechanisms and endothelial protection. PMID: 30291675 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 5, 2018 Category: Molecular Biology Authors: Kutryb-Zajac B, Koszalka P, Mierzejewska P, Bulinska A, Zabielska MA, Brodzik K, Skrzypkowska A, Zelazek L, Pelikant-Malecka I, Slominska EM, Smolenski RT Tags: J Cell Mol Med Source Type: research

Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential.
Abstract Hepatocellular carcinoma (HCC) is one of the most common causes for cancer-related death worldwide with rapidly increasing incidence and mortality rates. As for other types of cancers, also in HCC cancer stem cells (CSCs) are thought to be responsible for tumour initiation, progression and therapy failure. However, as rare subpopulations of tumour tissue, CSCs are difficult to isolate, thus making the development of suitable and reliable model systems necessary. In our study, we generated HepG2 subclones with enriched CSC potential by application of the spheroid formation method and subsequent single-cell...
Source: J Cell Mol Med - October 2, 2018 Category: Molecular Biology Authors: Muenzner JK, Kunze P, Lindner P, Polaschek S, Menke K, Eckstein M, Geppert CI, Chanvorachote P, Baeuerle T, Hartmann A, Schneider-Stock R Tags: J Cell Mol Med Source Type: research

Corrigendum.
Authors: PMID: 30255618 [PubMed - in process] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 27, 2018 Category: Molecular Biology Tags: J Cell Mol Med Source Type: research

Corrigendum.
Authors: PMID: 30255619 [PubMed - in process] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 27, 2018 Category: Molecular Biology Tags: J Cell Mol Med Source Type: research

Mitochondrial dysfunction contributes to the senescent phenotype of IPF lung fibroblasts.
In conclusion, increased superoxide production by dysfunctional mitochondria reinforces lung fibroblast senescence via prolongation of the DDR. As part of an auto-amplifying loop, mTORC1 is activated, altering mitochondrial homoeostasis and increasing superoxide production. Deeper understanding the mechanisms by which mitochondria contribute to fibroblast senescence in IPF has potentially important therapeutic implications. PMID: 30255990 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 26, 2018 Category: Molecular Biology Authors: Schuliga M, Pechkovsky DV, Read J, Waters DW, Blokland KEC, Reid AT, Hogaboam CM, Khalil N, Burgess JK, Prêle CM, Mutsaers SE, Jaffar J, Westall G, Grainge C, Knight DA Tags: J Cell Mol Med Source Type: research

Disseminated tumour cells in bone marrow are the source of cancer relapse after therapy.
Abstract Accumulating evidence indicates that cancer cells spread much earlier than was previously believed. Recent technological advances have greatly improved the detection methods of circulating tumour cells (CTCs), suggesting that the dissemination of cancer cells into the circulation occurs randomly. Most CTCs die in circulation as a result of shear stress and/or anoikis. However, the persistence of disseminated tumour cells (DTCs) in the bone marrow is the result of interaction of DTCs with bone marrow microenvironment. DTCs in the bone marrow undergo successive clonal expansions and a parallel progression t...
Source: J Cell Mol Med - September 26, 2018 Category: Molecular Biology Authors: Sai B, Xiang J Tags: J Cell Mol Med Source Type: research

Follistatin-like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase-dependent apoptosis and regulating Bcl-2 family proteins.
Abstract Hepatocellular carcinoma (HCC) is one of the most common and deadly malignant tumors in the world, especially in China. Follistatin-like protein 5 (FSTL5) is a member of the FSTL family, which is involved in cell proliferation, migration, differentiation, and embryo development. We aimed to investigate the function and underlying mechanism of FSTL5 in HCC. FSTL5 expression was determined by immunohistochemistry staining in a liver cancer tissue microarray (TMA) and the correlation between FSTL5 and the prognosis of HCC patients was analysed. Further proliferation assay, colony formation assay, flow cytome...
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Li C, Dai L, Zhang J, Zhang Y, Lin Y, Cheng L, Tian H, Zhang X, Wang Q, Yang Q, Wang Y, Shi G, Cheng F, Su X, Yang Y, Zhang S, Yu D, Wei Y, Deng H Tags: J Cell Mol Med Source Type: research

Increase of PRPP enhances chemosensitivity of PRPS1 mutant acute lymphoblastic leukemia cells to 5-Fluorouracil.
In this study, we found that PRPS1 mutant ALL cells specifically showed more chemosensitivity to 5-Fluorouracil (5-FU) than control cells, attributed to increased apoptosis of PRPS1 mutant cells by 5-FU. Mechanistically, PRPS1 mutants increase the level of intracellular phosphoribosyl pyrophosphate (PRPP), which causes the apt conversion of 5-FU to FUMP and FUTP in Reh cells, to promote 5-FU-induced DNA damage and apoptosis. Our study not only provides mechanistic rationale for re-targeting drug resistant cells in ALL, but also implicates that ALL patients who harbor relapse-specific mutations of PRPS1 might benefit from 5...
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Wang D, Chen Y, Fang H, Zheng L, Li Y, Yang F, Xu Y, Du L, Zhou BS, Li H Tags: J Cell Mol Med Source Type: research

Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells.
Abstract Deguelin, a natural rotenoid isolated from several plants, has been reported to exert anti-tumour effects in various cancers. However, the molecular mechanism of this regulation remains to be fully elucidated. Here, we found that deguelin inhibited the growth of non-small cell lung cancer (NSCLC) cells both in vitro and in vivo by downregulation of Bmi1 expression. Our data showed that Bmi1 is highly expressed in human NSCLC tissues and cell lines. Knockdown of Bmi1 significantly suppressed NSCLC cell proliferation and colony formation. Deguelin treatment attenuated the binding activity of Bmi1 ...
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Li W, Yu X, Xia Z, Yu X, Xie L, Ma X, Zhou H, Liu L, Wang J, Yang Y, Liu H Tags: J Cell Mol Med Source Type: research

Young bone marrow Sca-1 cells protect aged retina from ischaemia-reperfusion injury through activation of FGF2.
This study investigated whether young BM stem cells can regenerate and repair the aged retina after acute I/R injury. Young BM stem cell antigen 1 positive (Sca-1+ ) or Sca-1- cells were transplanted into lethally irradiated aged recipient mice to generate Sca-1+ and Sca-1- chimaeras, respectively. The animals were housed for 3 months to allow the young Sca-1 cells to repopulate in the BM of aged mice. Retinal I/R was then induced by elevation of intraocular pressure. Better preservation of visual function was found in Sca-1+ than Sca-1- chimaeras 7 days after injury. More Sca-1+ cells homed to the retina than Sca-1- cells...
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Shao Z, Wu J, Du G, Song H, Li SH, He S, Li J, Wu J, Weisel RD, Yuan H, Li RK Tags: J Cell Mol Med Source Type: research

Hypothesis: Caco-2 cell rotational 3D mechanogenomic turing patterns have clinical implications to colon crypts.
Abstract Colon crypts are recognized as a mechanical and biochemical Turing patterning model. Colon epithelial Caco-2 cell monolayer demonstrated 2D Turing patterns via force analysis of apical tight junction live cell imaging which illuminated actomyosin meshwork linking the actomyosin network of individual cells. Actomyosin forces act in a mechanobiological manner that alters cell/nucleus/tissue morphology. We observed the rotational motion of the nucleus in Caco-2 cells that appears to be driven by actomyosin during the formation of a differentiated confluent epithelium. Single- to multi-cell ring/torus-shaped ...
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Zheng G, Kalinin AA, Dinov ID, Meixner W, Zhu S, Wiley JW Tags: J Cell Mol Med Source Type: research

LAPTM4B gene polymorphism augments the risk of cancer: Evidence from an updated meta-analysis.
PMID: 30255662 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 25, 2018 Category: Molecular Biology Authors: Hashemi M, Bahari G, Tabasi F, Markowski J, Małecki A, Ghavami S, Łos MJ Tags: J Cell Mol Med Source Type: research

Galectin-3 promotes CXCR2 to augment the stem-like property of renal cell carcinoma.
In this study, we grew a subpopulation of RCC cells as tumour spheres with higher levels of stemness-related genes, such as Oct4, Sox2 and Nanog. Among the Gal family, Gal-3 in particular was highly expressed in RCC tumour spheres. To further investigate Gal-3's role in the stemness of RCC, lentivirus-mediated knockdown and overexpression of Gal-3 in RCC cells were used to examine both in vitro and in vivo tumorigenicity. We further assessed Gal-3 expression in RCC tissue microarray using immunohistochemistry. Upon suppressing Gal-3 in parental RCC cells, invasion, colony formation, sphere-forming ability, drug r...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Huang CS, Tang SJ, Lee MH, Chang Wang CC, Sun GH, Sun KH Tags: J Cell Mol Med Source Type: research

Meridianin C inhibits the growth of YD-10B human tongue cancer cells through macropinocytosis and the down-regulation of Dickkopf-related protein-3.
Abstract Meridianin C is a marine natural product known for its anti-cancer activity. At present, the anti-tumour effects of meridianin C on oral squamous cell carcinoma are unknown. Here, we investigated the effect of meridianin C on the proliferation of four different human tongue cancer cells, YD-8, YD-10B, YD-38 and HSC-3. Among the cells tested, meridianin C most strongly reduced the growth of YD-10B cells; the most aggressive and tumorigenic of the cell lines tested. Strikingly, meridianin C induced a significant accumulation of macropinosomes in the YD-10B cells; confirmed by the microscopic and TEM analysi...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Park NS, Park YK, Ramalingam M, Yadav AK, Cho HR, Hong VS, More KN, Bae JH, Bishop-Bailey D, Kano J, Noguchi M, Jang IS, Lee KB, Lee J, Choi JS, Jang BC Tags: J Cell Mol Med Source Type: research

Long non-coding RNA CRNDE promotes the proliferation, migration and invasion of hepatocellular carcinoma cells through miR-217/MAPK1 axis.
Abstract Hepatocellular carcinoma (HCC) is an invasive malignant tumour and the second major cause of cancer-related deaths over the world. CRNDE and miR-217 are non-coding RNAs which play critical roles in cell growth, proliferation, migration. Mitogen-activated protein kinase 1 (MAPK1) also participates in cancer cell process. Hence, this study aimed at investigating the effect of CRNDE on migration and invasion of HCC and figuring out the role of miR-217 and MAPK1 in this process. The overexpression of CRNDE was demonstrated by a microarray-based lncRNA profiling study. CRNDE expression in HCC was verified by q...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Wang H, Ke J, Guo Q, Barnabo Nampoukime KP, Yang P, Ma K Tags: J Cell Mol Med Source Type: research

High glucose-induced complement component 3 up-regulation via RAGE-p38MAPK-NF- κB signalling in astrocytes: In vivo and in vitro studies.
High glucose-induced complement component 3 up-regulation via RAGE-p38MAPK-NF-κB signalling in astrocytes: In vivo and in vitro studies. J Cell Mol Med. 2018 Sep 24;: Authors: Zhao Y, Luo C, Chen J, Sun Y, Pu D, Lv A, Zhu S, Wu J, Wang M, Zhou J, Liao Z, Zhao K, Xiao Q Abstract Diabetes is considered as a risk for cognitive decline, which is characterized by neurodegenerative alteration and innate immunity activation. Recently, complement 3 (C3), the critical central component of complement system, has been reported to play a key role in neurodegenerative alterations under pathological condition...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Zhao Y, Luo C, Chen J, Sun Y, Pu D, Lv A, Zhu S, Wu J, Wang M, Zhou J, Liao Z, Zhao K, Xiao Q Tags: J Cell Mol Med Source Type: research

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.
Abstract Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The Gpx1, Gpx4 and SelS have different binding affinities with SBP2 in cells. According to our results, both miR-181a-5p and SBP2 appeared to be selenium-sensitive and regulated the expression of selenoproteins in C28/I2 cells under Se sufficient environment. However, they showed significa...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Min Z, Guo Y, Sun M, Hussain S, Zhao Y, Guo D, Huang H, Heng L, Zhang F, Ning Q, Han Y, Xu P, Zhong N, Sun J, Lu S Tags: J Cell Mol Med Source Type: research

C5aR1 interacts with TLR2 in osteoblasts and stimulates the osteoclast-inducing chemokine CXCL10.
In conclusion, C5a-stimulated osteoblasts might modulate osteoclast activity and contribute to immunomodulation in inflammatory bone disorders. PMID: 30247799 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Mödinger Y, Rapp A, Pazmandi J, Vikman A, Holzmann K, Haffner-Luntzer M, Huber-Lang M, Ignatius A Tags: J Cell Mol Med Source Type: research

NEK4 kinase regulates EMT to promote lung cancer metastasis.
Abstract Epithelial-to-mesenchymal transition (EMT) is a dynamic transitional state from the epithelial to mesenchymal phenotypes. Numerous studies have suggested that EMT and its intermediate states play important roles in tumor invasion and metastasis. To identify novel regulatory molecules of EMT, we screened a siRNA library targeting human 720 kinases in A549 lung adenocarcinoma cells harboring E-cadherin promoter-luciferase reporter vectors. NIMA-related kinase-4 (NEK4) was identified and characterized as a positive regulator of EMT in the screening. Suppression of NEK4 resulted in the inhibition of cell migr...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Ding NH, Zhang L, Xiao Z, Rong ZX, Li Z, He J, Chen L, Ou DM, Liao WH, Sun LQ Tags: J Cell Mol Med Source Type: research

Inhibition of autophagy enhances the antitumour activity of tigecycline in multiple myeloma.
Abstract Accumulating evidence shows that tigecycline, a first-in-class glycylcycline, has potential antitumour properties. Here, we found that tigecycline dramatically inhibited the proliferation of multiple myeloma (MM) cell lines RPMI-8226, NCI-H929 and U266 in a dose and time-dependent manner. Meanwhile, tigecycline also potently impaired the colony formation of these three cell lines. Mechanism analysis found that tigecycline led to cell cycle arrest at G0/G1 with down-regulation of p21, CDK2 and cyclin D1, rather than induced apoptosis, in MM cells. Importantly, we found that tigecycline induced autophagy an...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Ma R, Zhang Y, Wang W, Wu J, Yang Q, Xu W, Jiang S, Han Y, Yu K, Zhang S Tags: J Cell Mol Med Source Type: research

Regulation of proliferation and invasion by the IGF signalling pathway in Epstein-Barr virus-positive gastric cancer.
In conclusion, AGS-EBV cells seem to modulate their proliferation and invasion through the IGF signalling pathway. Inhibition of the IGF signalling pathway therefore could be a potential therapeutic strategy for EBVaGC. PMID: 30247804 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Jeong I, Kang SK, Kwon WS, Kim HJ, Kim KH, Kim HM, Lee A, Lee SK, Bogenrieder T, Chung HC, Rha SY Tags: J Cell Mol Med Source Type: research

An alternative renin isoform is cardioprotective by modulating mitochondrial metabolism.
Abstract The renin-angiotensin system promotes oxidative stress, apoptosis, necrosis, fibrosis, and thus heart failure. Secretory renin plays a central role in these processes, initiating the generation of angiotensins. Nevertheless, alternative renin transcripts exist, which code for a cytosolically localized renin isoform (cyto-renin) that is cardioprotective. We tested the hypothesis that the protective effects are associated with a beneficial switch of metabolic and mitochondrial functions. To assess H9c2 cell mitochondrial parameters, we used the Seahorse XF analyser. Cardiac H9c2 cells overexpressing cyto-reni...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Wanka H, Lutze P, Staar D, Grunow B, Peters BS, Peters J Tags: J Cell Mol Med Source Type: research

A four-gene-based prognostic model predicts overall survival in patients with hepatocellular carcinoma.
Abstract With the development of new advances in hepatocellular carcinoma (HCC) management and noninvasive radiological techniques, high-risk patient groups such as those with hepatitis virus are closely monitored. HCC is increasingly diagnosed early, and treatment may be successful. In spite of this progress, most patients who undergo a hepatectomy will eventually relapse, and the outcomes of HCC patients remain unsatisfactory. In our study, we aimed to identify potential gene biomarkers based on RNA sequencing data to predict and improve HCC patient survival. The gene expression data and clinical information wer...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Long J, Zhang L, Wan X, Lin J, Bai Y, Xu W, Xiong J, Zhao H Tags: J Cell Mol Med Source Type: research

PKC α replaces AMPK to regulate mitophagy: Another PEDF role on ischaemic cardioprotection.
This study therefore identifies a previously uncharacterized interaction between the ULK1 and PKCα that can replace the AMPK-dependent mitophagy processes. PMID: 30230261 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 19, 2018 Category: Molecular Biology Authors: Miao H, Qiu F, Huang B, Liu X, Zhang H, Liu Z, Yuan Y, Zhao Q, Zhang H, Dong H, Zhang Z Tags: J Cell Mol Med Source Type: research

Heme oxygenase-1 induction mediates chemoresistance of breast cancer cells to pharmorubicin by promoting autophagy via PI3K/Akt pathway.
CONCLUSION: It was proved that HO-1 induction mediated chemoresistance of pharmorubicin in breast cancer cells by promoting autophagy via PI3K/Akt pathway. PMID: 30216645 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 14, 2018 Category: Molecular Biology Authors: Pei L, Kong Y, Shao C, Yue X, Wang Z, Zhang N Tags: J Cell Mol Med Source Type: research

Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound-activated interferon- γ/interleukin-10 axis.
Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound-activated interferon-γ/interleukin-10 axis. J Cell Mol Med. 2018 Sep 14;: Authors: Burks SR, Nagle ME, Bresler MN, Kim SJ, Star RA, Frank JA Abstract Mesenchymal stromal cell (MSC) therapies combined with renal pulsed focused ultrasound (pFUS) pretreatment increase MSC homing and improve cisplatin-induced acute kidney injury (AKI) better than MSC alone. However, mechanisms underlying improved outcomes remain unknown. We hypothesize pFUS up-regulates renal interferon-γ (IFNγ) and stimulates MSC to pr...
Source: J Cell Mol Med - September 14, 2018 Category: Molecular Biology Authors: Burks SR, Nagle ME, Bresler MN, Kim SJ, Star RA, Frank JA Tags: J Cell Mol Med Source Type: research

Cardioprotective cytokine interleukin-33 is up-regulated by statins in human cardiac tissue.
Abstract Interleukin (IL)-33 is a member of the IL-1 family and is able to act cardioprotective. The aim of this study was to investigate the regulation of IL-33 by 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase inhibitors (statins) and bisphosphonates (BPs) in human cardiac tissue. The lipophilic fluvastatin, simvastatin, atorvastatin, and lovastatin as well as the nitrogenous BPs alendronate and ibandronate, but not hydrophilic pravastatin increased IL-33 mRNA and intracellular IL-33 protein levels in both human adult cardiac myocytes (HACM) and fibroblasts (HACF). Additionally, fluvastatin reduced so...
Source: J Cell Mol Med - September 14, 2018 Category: Molecular Biology Authors: Pentz R, Kaun C, Thaler B, Stojkovic S, Lenz M, Krychtiuk KA, Zuckermann A, Huber K, Wojta J, Hohensinner PJ, Demyanets S Tags: J Cell Mol Med Source Type: research

PCAF fine-tunes hepatic metabolic syndrome, inflammatory disease, and cancer.
Abstract The P300/CBP-associating factor (PCAF), a histone acetyltransferase, is involved in metabolic and pathogenic diseases, particularly of the liver. The effects of PCAF on fine-tuning liver diseases are extremely complex and vary according to different pathological conditions. This enzyme has dichotomous functions, depending on differently modified sites, which regulate the activities of various enzymes, metabolic functions, and gene expression. Here, we summarize the most recent findings on the functions and targets of PCAF in various metabolic and immunological processes in the liver and review these new d...
Source: J Cell Mol Med - September 14, 2018 Category: Molecular Biology Authors: Wang T, Yao W, Shao Y, Zheng R, Huang F Tags: J Cell Mol Med Source Type: research

Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease.
Abstract Intervertebral discs (IVD) degeneration, which is caused by ageing or mechanical stress, leads to IVD disease, including back pain and sciatica. The cytokine interleukin (IL)-17A is elevated in NP cells during IVD disease. Here we explored the pharmacotherapeutic potential of IL-17A for the treatment of IVD disease using small-molecule inhibitors that block binding of IL-17A to the IL-17A receptor (IL-17RA). Treatment of NP cells with IL-17A increased expression of cyclooxygenase-2 (COX-2), IL-6, matrix metalloproteinase (MMP)-3 and MMP-13. These increases were suppressed by an IL-17A-neutralizing antibod...
Source: J Cell Mol Med - September 11, 2018 Category: Molecular Biology Authors: Suyama K, Sakai D, Hirayama N, Nakamura Y, Matsushita E, Terayama H, Qu N, Tanaka O, Sakabe K, Watanabe M Tags: J Cell Mol Med Source Type: research

Association between the T6459C point mutation of the mitochondrial MT-CO1 gene and susceptibility to sepsis among Chinese Han people.
In this study, a proband harbouring mitochondrial T6459C mutation with sepsis and his Chinese Han pedigree including 7 members of 3 generations were enrolled. General information, blood parameters and mitochondrial full sequence scanning of all members were performed, and cellular functions, including cellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), degrees of cell apoptosis and adenosine triphosphate (ATP) concentrations, were measured in members with and without the T6459C mutation. Through mitochondrial full sequence scanning and analysis of all members we found, the maternal member...
Source: J Cell Mol Med - September 11, 2018 Category: Molecular Biology Authors: Shen X, Han G, Li S, Song Y, Shen H, Zhai Y, Wang Y, Zhang F, Dong N, Li T, Yao Y, Zhu H Tags: J Cell Mol Med Source Type: research

Chronic simian immunodeficiency virus infection is associated with contrasting phenotypes of dysfunctional Bcl6+ germinal center B cells or Bcl6- Bcl2+ non-germinal center B cells.
Abstract Human immunodeficiency virus (HIV) infection is characterized by dysfunctional B cell responses. Here we show that chronic simian immunodeficiency virus (SIV) infection is characterized by an expansion of either lymph node germinal center (GC) B cells that co-express Bcl6, Ki-67 and IL-21R and correlate with expanded T follicular helper (Tfh) cells or B cells that lack Bcl6, Ki-67 and IL-21R but express high levels of anti-apoptotic Bcl2 that negatively correlate with Tfh cells. The lack of Tfh cells likely contributes to persistence of dysfunctional non-proliferating B cells during chronic infection. The...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Onabajo OO, Lewis MG, Mattapallil JJ Tags: J Cell Mol Med Source Type: research

Correcting "insertion-deletion mutations" in medical terminology.
Correcting "insertion-deletion mutations" in medical terminology. J Cell Mol Med. 2018 Sep 06;: Authors: Mentis AA, Papavassiliou AG PMID: 30187998 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Mentis AA, Papavassiliou AG Tags: J Cell Mol Med Source Type: research

Plectin protects podocytes from adriamycin-induced apoptosis and F-actin cytoskeletal disruption through the integrin α6β4/FAK/p38 MAPK pathway.
In this study, we found that adriamycin (ADR) treatment resulted in typical podocyte injury and repressed plectin expression. Restoring plectin expression protected against ADR-induced podocyte injury whereas siRNA-mediated plectin silencing produced similar effects as ADR-induced podocyte injury, suggesting that plectin plays a key role in preventing podocyte injury. Further analysis showed that plectin repression induced significant integrin α6β4, focal adhesion kinase (FAK) and p38 MAPK phosphorylation. Mutating Y1494, a key tyrosine residue in the integrin β4 subunit, blocked FAK and p38 phosphorylation...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Ni Y, Wang X, Yin X, Li Y, Liu X, Wang H, Liu X, Zhang J, Gao H, Shi B, Zhao S Tags: J Cell Mol Med Source Type: research

Epithelial down-regulation of the miR-200 family in fibrostenosing Crohn's disease is associated with features of epithelial to mesenchymal transition.
Abstract Intestinal mesenchymal cells deposit extracellular matrix in fibrotic Crohn's disease (CD). The contribution of epithelial to mesenchymal transition (EMT) to the mesenchymal cell pool in CD fibrosis remains obscure. The miR-200 family regulates fibrosis-related EMT in organs other than the gut. E-cadherin, cytokeratin-18 and vimentin expression was assessed using immunohistochemistry on paired strictured (SCD) and non-strictured (NSCD) ileal CD resections and correlated with fibrosis grade. MiR-200 expression was measured in paired SCD and NSCD tissue compartments using laser capture microdissection and R...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Mehta SJ, Lewis A, Nijhuis A, Jeffery R, Biancheri P, Di Sabatino A, Feakins R, Silver A, Lindsay JO Tags: J Cell Mol Med Source Type: research

The novel regulatory role of lncRNA-miRNA-mRNA axis in cardiovascular diseases.
Abstract Long noncoding RNAs (lncRNAs) are RNAs longer than 200 nt in length that are characterized by low levels of sequence conservation and expression; lncRNAs modulate various biological functions at epigenetic, transcriptional and post-transcriptional levels, or directly regulate protein activity. As a family of small and evolutionarily conserved noncoding RNAs, microRNAs (miRNAs) are capable of regulating physiological and pathological processes via inhibiting target mRNA translation or promoting mRNA degradation. A number of studies have confirmed that both lncRNAs and miRNAs are closely associated with the...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Huang Y Tags: J Cell Mol Med Source Type: research

Hiding in Plain Sight: Nebivolol Exhibits Compelling Tocolytic Properties.
Abstract Preterm birth before 37 weeks of completed gestation results in numerous health consequences for the foetus. Preterm labour leads to preterm birth in over 50% of cases, and no FDA-approved treatment can prevent labour or help a foetus remain in the womb until term. Examination of nitric oxide mediated relaxation signaling in the uterine smooth muscle reveals a role for protein S-nitrosation. The recent discovery of upregulated S-nitrosoglutathione reductase (GSNOR) in spontaneously preterm labouring women has emphasized the need to explore the function of S-nitrosation regulation in the maintenance of ute...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Barnett SD, Buxton ILO Tags: J Cell Mol Med Source Type: research

The potential contribution and role of a blood platelets in autoimmune thyroid diseases.
Abstract The blood platelets are multifunctional blood cells which are involved in the initiation of atheroma, endothelial dysfunction, and modulation of inflammatory and immune responses in the pathophysiology of many diseases. Because of their multifaceted pro-inflammatory activity, platelets may be involved in the pathogenesis of autoimmune thyroid diseases (AITDs), such as Hashimoto's thyroiditis and Graves' disease. The aim of this study was to assess the level of activation and response ability of platelets in AITDs. We used the flow cytometry technique and kinetic measurement of aggregation to analyse plate...
Source: J Cell Mol Med - September 6, 2018 Category: Molecular Biology Authors: Tomczyńska M, Salata I, Bijak M, Saluk-Bijak J Tags: J Cell Mol Med Source Type: research

miR-21 modification enhances the performance of adipose tissue-derived mesenchymal stem cells for counteracting urethral stricture formation.
This study investigated whether miR-21 modification can improve the therapeutic efficacy of ADSCs against urethral fibrosis to limit urethral stricture recurrence. MiR-21-modified ADSCs (miR-21) were constructed via lentivirus-mediated transfer of pre-miR-21 and GFP reporter gene. In vitro results suggested that miR-21 modification can increase the angiogenesis genes expression of ADSCs and enhance its anti-oxidative effects against reactive oxygen species (ROS) damage. In vivo results showed that miR-21 modification contributes to increased urodynamic parameters and better formation of the epithelium and the muscle layer ...
Source: J Cell Mol Med - September 4, 2018 Category: Molecular Biology Authors: Feng Z, Chen H, Fu T, Zhang L, Liu Y Tags: J Cell Mol Med Source Type: research

Isobavachalcone sensitizes cells to E2-induced paclitaxel resistance by down-regulating CD44 expression in ER+ breast cancer cells.
Abstract Oestrogen receptor (ER) is expressed in approximately 60%-70% of human breast cancer. Clinical trials and retrospective analyses have shown that ER-positive (ER+) tumours are more tolerant to chemotherapeutic drug resistance than ER-negative (ER-) tumours. In addition, isobavachalcone (IBC) is known as a kind of phytoestrogen with antitumour effect. However, the underlying mechanism of IBC in ER+ breast cancer needs to be elucidated further. Our in vitro experiments showed that IBC could attenuate 17β-estradiol (E2 )-induced paclitaxel resistance and that E2 could stimulate CD44 expression in ER...
Source: J Cell Mol Med - September 4, 2018 Category: Molecular Biology Authors: Shi J, Chen Y, Chen W, Tang C, Zhang H, Chen Y, Yang X, Xu Z, Wei J, Chen J Tags: J Cell Mol Med Source Type: research

H1/pAIM2 nanoparticles exert anti-tumour effects that is associated with the inflammasome activation in renal carcinoma.
Abstract Renal cell carcinoma (RCC) is a high metastasis tumour with less effective treatment available currently. Absent in melanoma 2 (AIM2) as a tumour suppressor might be used as a potential therapeutic target for RCC treatment. Here, we found that AIM2 expression was significantly decreased in RCC patient specimens and renal carcinoma cell lines (786-O and OSRC-2). To establish a safe and effective AIM2 gene delivery system, we formed the nanoparticles consisting of a folate grafted PEI600-CyD (H1) nanoparticle-mediated AIM2 gene (H1/pAIM2) as an effective delivery agent. Delivery of H1/pAIM2 in renal carcino...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Chai D, Liu N, Li H, Wang G, Song J, Fang L, Lu Z, Yao H, Zheng J Tags: J Cell Mol Med Source Type: research

Polarized retinal pigment epithelium generates electrical signals that diminish with age and regulate retinal pathology.
In conclusion, the TEP across the RPE depends on the expression of ATP1B1 and this regulates the secretion of PEDF by RPE cells and so may regulate the onset of retinal disease. Increasing the expression of PEDF using an applied EF to replenish a disease or age-reduced TEP may offer a new way of preventing or reversing retinal dysfunction. PMID: 30160348 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Cao L, Liu J, Pu J, Milne G, Chen M, Xu H, Shipley A, Forrester JV, McCaig CD, Lois N Tags: J Cell Mol Med Source Type: research

Helicobacter pylori-induced exosomal MET educates tumour-associated macrophages to promote gastric cancer progression.
In this study, we investigated exosome-mediated communication between H. pylori-infected GC cells and macrophages, focusing on the transfer of activated mesenchymal-epithelial transition factor (MET). We observed a significant decrease in MET protein expression in GC cells after infection with H. pylori, whereas MET mRNA levels remained unchanged. Intriguingly, MET expression, specifically the phosphorylated active form, was increased in exosomes released from H. pylori-infected GC cells. Confocal microscopy and Western blotting analyses showed that these exosomes containing MET were delivered to and interna...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Che Y, Geng B, Xu Y, Miao X, Chen L, Mu X, Pan J, Zhang C, Zhao T, Wang C, Li X, Wen H, Liu Z, You Q Tags: J Cell Mol Med Source Type: research

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex  vivo and in vivo.
Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo. J Cell Mol Med. 2018 Aug 30;: Authors: Hu C, Zhao L, Peng C, Li L Abstract Mesenchymal stem cells (MSCs) are broadly used in cell-based regenerative medicine because of their self-renewal and multilineage potencies in vitro and in vivo. To ensure sufficient amounts of MSCs for therapeutic purposes, cells are generally cultured in vitro for long-term expansion or specific terminal differentiation until cell transplantation. Although physiologically up-...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Hu C, Zhao L, Peng C, Li L Tags: J Cell Mol Med Source Type: research

A novel, homozygous nonsense variant of the CDHR1 gene in a Chinese family causes autosomal recessive retinal dystrophy by NGS-based genetic diagnosis.
In conclusion, our study is the first to indicate that the novel homozygous variant c.T1641A (p.Y547*) in the CHDR1 gene might be the disease-causing mutation for retinal dystrophy in our patient, extending its mutation spectrums. These findings further the understanding of the molecular pathogenesis of this disease and provide new insights for diagnosis as well as new implications for genetic counselling. PMID: 30160356 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Fu J, Ma L, Cheng J, Yang L, Wei C, Fu S, Lv H, Chen R, Fu J Tags: J Cell Mol Med Source Type: research

Sprouty2 suppresses progression and correlates to favourable prognosis of intrahepatic cholangiocarcinoma via antagonizing FGFR2 signalling.
In conclusion, SPRY2 was correlated with favourable prognosis of ICC via suppressing FGFR2-induced ERK phosphorylation, invasion and EMT. The phosphorylation of SPRY2-Y55 was required in this tumour-suppressing function of SPRY2. PMID: 30160357 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Xu YF, Liu HD, Liu ZL, Pan C, Yang XQ, Ning SL, Zhang ZL, Guo S, Yu JM Tags: J Cell Mol Med Source Type: research

SCN1B β mutations that affect their association with Kv4.3 underlie early repolarization syndrome.
SCN1Bβ mutations that affect their association with Kv4.3 underlie early repolarization syndrome. J Cell Mol Med. 2018 Aug 30;: Authors: Yao H, Fan J, Cheng YJ, Chen XM, Ji CC, Liu LJ, Zheng ZH, Wu SH Abstract BACKGROUND: Abnormal cardiac ion channels current, including transient outward potassium current (Ito ), is associated with early repolarization syndrome (ERS). Previous studies showed that mutations in SCN1Bβ both to increase the Ito current and to decrease the sodium current. Yet its role in ERS remains unknown. OBJECTIVE: To determine the role of mutations in the SCN1Bβ su...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Yao H, Fan J, Cheng YJ, Chen XM, Ji CC, Liu LJ, Zheng ZH, Wu SH Tags: J Cell Mol Med Source Type: research

The caveolae-associated coiled-coil protein, NECC2, regulates insulin signalling in Adipocytes.
n S, Vitale N, Jiménez-Gómez Y, Malagón MM Abstract Adipocyte dysfunction in obesity is commonly associated with impaired insulin signalling in adipocytes and insulin resistance. Insulin signalling has been associated with caveolae, which are coated by large complexes of caveolin and cavin proteins, along with proteins with membrane-binding and remodelling properties. Here, we analysed the regulation and function of a component of caveolae involved in growth factor signalling in neuroendocrine cells, neuroendocrine long coiled-coil protein-2 (NECC2), in adipocytes. Studies in 3T3-L1 cells show...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Trávez A, Rabanal-Ruiz Y, López-Alcalá J, Molero-Murillo L, Díaz-Ruiz A, Guzmán-Ruiz R, Catalán V, Rodríguez A, Frühbeck G, Tinahones FJ, Gasman S, Vitale N, Jiménez-Gómez Y, Malagón MM Tags: J Cell Mol Med Source Type: research

Timing of erythropoietin modified mesenchymal stromal cell transplantation for the treatment of experimental bronchopulmonary dysplasia.
Abstract The aim of this study is to optimize the timing of erythropoietin gene modified mesenchymal stem cells (EPO-MSCs) transplantation for bronchopulmonary dysplasia (BPD). Three weeks post-operation, the results indicated that the damage of airway structure and apoptosis were significantly decreased, the proliferation was increased in three EPO-MSCs transplantation groups as compared with BPD mice. Moreover, the inflammation cytokines were improvement in early EPO-MSCs injection mice than in BPD mice, but there was no significant difference between late injection and BPD groups. Furthermore, the protein expre...
Source: J Cell Mol Med - August 30, 2018 Category: Molecular Biology Authors: Zhang Z, Sun C, Wang J, Jiang W, Xin Q, Luan Y Tags: J Cell Mol Med Source Type: research

Pharmacological characterization of hetrombopag, a novel orally active human thrombopoietin receptor agonist.
Abstract Nonpeptide thrombopoietin receptor (TPOR/MPL) agonists, such as eltrombopag, have been used to treat thrombocytopenia of various aetiologies. Here, we investigated the pharmacological properties of hetrombopag, a new orally active small-molecule TPOR agonist, in preclinical models. Hetrombopag specifically stimulated proliferation and/or differentiation of human TPOR-expressing cells, including 32D-MPL and human hematopoietic stem cells, with low nanomolar EC50 values through stimulation of STAT, PI3K and ERK signalling pathways. Notably, hetrombopag effectively up-regulated G1 -phase-related proteins, in...
Source: J Cell Mol Med - August 29, 2018 Category: Molecular Biology Authors: Xie C, Zhao H, Bao X, Fu H, Lou L Tags: J Cell Mol Med Source Type: research