Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model
In this study, we prepared exosomes (EXOs) from cartilage stem/progenitor cells (CSPCs) and found that treatment with EXOs increased the viability, migration, and proliferation of cultured primary chondrocytes. In a subacute OA rat model, the application of EXOs facilitated cartilage regeneration as evidenced by histological staining. Exosomal protein analysis together with bioinformatics suggested that cyclin-dependent kinase 9 (CDK9) is a key factor for chondrocyte growth and migration. Functional studies confirmed this prediction, that is, inhibiting CDK9 reduced the beneficial effects induced by EXOs in primary chondro...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Jing Chen Xiaohui Ni Jian Yang Hongwei Yang Xiaoyu Liu Minhao Chen Cheng Sun Youhua Wang Source Type: research
Detecting early-warning biomarkers associated with heart-exosome genetic-signature for acute myocardial infarction: A source-tracking study of exosome
J Cell Mol Med. 2024 Apr;28(8):e18334. doi: 10.1111/jcmm.18334.ABSTRACTThe genetic information of plasma total-exosomes originating from tissues have already proven useful to assess the severity of coronary artery diseases (CAD). However, plasma total-exosomes include multiple sub-populations secreted by various tissues. Only analysing the genetic information of plasma total-exosomes is perturbed by exosomes derived from other organs except the heart. We aim to detect early-warning biomarkers associated with heart-exosome genetic-signatures for acute myocardial infarction (AMI) by a source-tracking analysis of plasma exoso...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Xiaojun Jin Weifeng Xu Qiaoping Wu Chen Huang Yongfei Song Jiangfang Lian Source Type: research
Retraction: Silencing of Prrx1b suppresses cellular proliferation, migration, invasion and epithelial-mesenchymal transition in triple-negative breast cancer
J Cell Mol Med. 2024 Apr;28(8):e18312. doi: 10.1111/jcmm.18312.ABSTRACTZhi-Dong Lv, Zhao-Chuan Yang, Xiang-Ping Liu, Li-Ying Jin, Qian Dong, Hui-Li Qu, Fu-Nian Li, Bin Kong, Jiao Sun, Jiao-Jiao Zhao, Hai-Bo Wang, Silencing of Prrx1b suppresses cellular proliferation, migration, invasion and epithelial-mesenchymal transition in triple-negative breast cancer. Journal of Cellular and Molecular Medicine, 20: 1640-1650. https://doi.org/10.1111/jcmm.12856 The above article, published online on 29 March 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-i...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Source Type: research
Anti-inflammatory and immunoregulatory effects of colistin sulphate on human PBMCs
J Cell Mol Med. 2024 Apr;28(8):e18322. doi: 10.1111/jcmm.18322.ABSTRACTIn previous studies, CST has been identified as having an immunostimulatory effect on Caenorhabditis elegans and macrophage of rats. Here, we further investigated its immunomodulatory effects on human peripheral blood mononuclear cells (PBMCs). LPS-stimulated PBMCs inflammatory model was established. Flow cytometry was applied to measure phagocytosis of PBMCs. Cytokine mRNA and protein expression levels of LPS-stimulated PBMCs with or without CST were measured by qRT-PCR and ELISA. The transcriptomic profile of CST-treated PBMCs was investigated by RNA-...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Huiling Chen Tianli Yang Yiran Xu Beibei Liang Xianyong Liu Yun Cai Source Type: research
KIAA0040 enhances glioma growth by controlling the JAK2/STAT3 signalling pathway
In this study, KIAA0040 expression levels were evaluated using qRT-PCR, WB and IHC, and functional assays were conducted to assess its impact on glioma progression, along with animal experiments. Moreover, WB was used to examine the impact of KIAA0040 on the JAK2/STAT3 signalling pathway. Our study found that KIAA0040 was increased in glioma and linked to tumour grade and poor clinical outcomes, serving as an independent prognostic factor. Functional assays showed that KIAA0040 enhances glioma growth, migration and invasion by activating the JAK2/STAT3 pathway. Of course, KIAA0040 enhances glioma growth by preventing tumou...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Jie He Kaming Xue Fei Fan Lin Li Xinyu Rao Wei Liu Chuansheng Nie Source Type: research
Retraction: miR-655 suppresses epithelial-to-mesenchymal transition by targeting Prrx1 in triple-negative breast cancer
J Cell Mol Med. 2024 Apr;28(8):e18314. doi: 10.1111/jcmm.18314.ABSTRACTZhi-Dong Lv, Bin Kong, Xiang-Ping Liu, Li-Ying Jin, Qian Dong, Fu-Nian Li, Hai-Bo Wang, miR-655 suppresses epithelial-to-mesenchymal transition by targeting Prrx1 in triple-negative breast cancer. Journal of Cellular and Molecular Medicine, 20: 864-873. https://doi.org/10.1111/jcmm.12770 The above article, published online on 28 January 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-in-Chief, Stefan Constantinescu, The Foundation for Cellular and Molecular Medicine, and John...
Source: J Cell Mol Med - April 24, 2024 Category: Molecular Biology Source Type: research
Retraction: miR-655 suppresses epithelial-to-mesenchymal transition by targeting Prrx1 in triple-negative breast cancer
J Cell Mol Med. 2024 Apr;28(8):e18314. doi: 10.1111/jcmm.18314.ABSTRACTZhi-Dong Lv, Bin Kong, Xiang-Ping Liu, Li-Ying Jin, Qian Dong, Fu-Nian Li, Hai-Bo Wang, miR-655 suppresses epithelial-to-mesenchymal transition by targeting Prrx1 in triple-negative breast cancer. Journal of Cellular and Molecular Medicine, 20: 864-873. https://doi.org/10.1111/jcmm.12770 The above article, published online on 28 January 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-in-Chief, Stefan Constantinescu, The Foundation for Cellular and Molecular Medicine, and John...
Source: J Cell Mol Med - April 24, 2024 Category: Molecular Biology Source Type: research
Retraction: Tanshinone IIA alleviates lipopolysaccharide-induced acute lung injury by downregulating TRPM7 and pro-inflammatory factors
J Cell Mol Med. 2024 Apr;28(8):e18313. doi: 10.1111/jcmm.18313.ABSTRACTJie Li, Yan Zheng, Ming-Xian Li, Chu-Wei Yang, Yu-Fei Liu, Tanshinone IIA alleviates lipopolysaccharide-induced acute lung injury by downregulating TRPM7 and pro-inflammatory factors. Journal of Cellular and Molecular Medicine, 22: 646-654. https://doi.org/10.1111/jcmm.13350 The above article, published online on 18 October 2017 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by the journal Editor-in-Chief, Stefan Constantinescu, The Foundation for Cellular and Molecular Medicine, and John Wiley and Sons Ltd. The retraction has been...
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Source Type: research
Macrophage-derived extracellular vesicles alter cardiac recovery and metabolism in a rat heart model of donation after circulatory death
J Cell Mol Med. 2024 Apr;28(8):e18281. doi: 10.1111/jcmm.18281.ABSTRACTConditions to which the cardiac graft is exposed during transplantation with donation after circulatory death (DCD) can trigger the recruitment of macrophages that are either unpolarized (M0) or pro-inflammatory (M1) as well as the release of extracellular vesicles (EV). We aimed to characterize the effects of M0 and M1 macrophage-derived EV administration on post-ischaemic functional recovery and glucose metabolism using an isolated rat heart model of DCD. Isolated rat hearts were subjected to 20 min aerobic perfusion, followed by 27 min global, warm i...
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Selianne Graf Vanessa Biemmi Maria Arnold Adrian Segiser Anja M üller Natalia M éndez-Carmona Manuel Egle Matthias Siepe Lucio Barile Sarah Longnus Source Type: research
Identification of PPAR-related differentially expressed genes liver hepatocellular carcinoma and construction of a prognostic model based on data analysis and molecular docking
In this study, single-cell RNA sequencing (scRNA-seq) data were used to explore the molecular mechanisms of LIHC development and identify potential targets for therapy. The expression of peroxisome proliferator-activated receptors (PPAR)-related genes was analysed in LIHC samples, and primary cell populations, including natural killer cells, T cells, B cells, myeloid cells, endothelial cells, fibroblasts and hepatocytes, were identified. Analysis of the differentially expressed genes (DEGs) between normal and tumour tissues revealed significant changes in gene expression in various cell populations. PPAR activity was evalu...
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Yumeng Wang Shuqiang Li Zihang Liu Xuanzheng Li Yifan Yu Hao Liu Source Type: research
Non-apoptotic regulated cell death mediates reprogramming of the tumour immune microenvironment by macrophages
J Cell Mol Med. 2024 Apr;28(8):e18348. doi: 10.1111/jcmm.18348.ABSTRACTTumour immune microenvironment (TIME) plays an indispensable role in tumour progression, and tumour-associated macrophages (TAMs) are the most abundant immune cells in TIME. Non-apoptotic regulated cell death (RCD) can avoid the influence of tumour apoptosis resistance on anti-tumour immune response. Specifically, autophagy, ferroptosis, pyroptosis and necroptosis mediate the crosstalk between TAMs and tumour cells in TIME, thus reprogram TIME and affect the progress of tumour. In addition, although some achievements have been made in immune checkpoint ...
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Chengpeng Sun Jianhao Zhan Yao Li Chulin Zhou Shuo Huang Xingen Zhu Kai Huang Source Type: research
An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma
This study investigated the role of FOXP1 in oesophageal squamous cell carcinoma through bioinformatics analysis and experimental verification. We determined through public databases that FOXP1 expresses low in oesophageal squamous cell carcinoma compared with normal tissues, while high expression of FOXP1 indicates a better prognosis. We identified potential target genes regulated by FOXP1, and explored the potential biological processes and signalling pathways involved in FOXP1 in oesophageal squamous cell carcinoma through GO and KEGG enrichment, gene co-expression analysis, and protein interaction network construction....
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Guanzhi Ye Gaojian Pan Xiaolei Zhu Ning Li Hongming Liu Guojun Geng Jie Jiang Source Type: research
Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers
J Cell Mol Med. 2024 Apr;28(8):e18122. doi: 10.1111/jcmm.18122.ABSTRACTBi-allelic variants in VWA1, encoding Von Willebrand Factor A domain containing 1 protein localized to the extracellular matrix (ECM), were linked to a neuromuscular disorder with manifestation in child- or adulthood. Clinical findings indicate a neuromyopathy presenting with muscle weakness. Given that pathophysiological processes are still incompletely understood, and biomarkers are still missing, we aimed to identify blood biomarkers of pathophysiological relevance: white blood cells (WBC) and plasma derived from six VWA1-patients were investigated b...
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Mohammed Athamneh Nassam Daya Andreas Hentschel Andrea Gangfuss Tobias Ruck Adela Della Marina Ulrike Schara-Schmidt Albert Sickmann Anne-Katrin G üttsches Marcus Deschauer Corinna Preusse Matthias Vorgerd Andreas Roos Source Type: research
Interplay of precision therapeutics and MD study: Calocybe indica's potentials against cervical cancer and its interaction with VEGF via octadecanoic acid
This study offers a holistic perspective on the promising therapeutic avenues facilitated by C. indica against cervical cancer, intricately woven with advanced molecular interactions and the prospective integration of precision therapeutics in modern oncology.PMID:38652115 | PMC:PMC11037404 | DOI:10.1111/jcmm.18302 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Suhana Datta Preeti Verma Bikram Dhara Rita Kundu Swastika Maitra Arup Kumar Mitra Mohd Shahnawaz Khan Torki A Zughaibi Shams Tabrez Ajoy Kumer Source Type: research
Rapid discrimination of four Salmonella enterica serovars: A performance comparison between benchtop and handheld Raman spectrometers
This study demonstrated that handheld Raman spectrometers achieved similar prediction accuracy as benchtop spectrometers when combined with machine learning models, providing an effective solution for rapid, accurate and cost-effective identification of closely associated S. enterica serovars.PMID:38652116 | PMC:PMC11037414 | DOI:10.1111/jcmm.18292 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 23, 2024 Category: Molecular Biology Authors: Quan Yuan Bin Gu Wei Liu Xin-Ru Wen Ji-Liang Wang Jia-Wei Tang Muhammad Usman Su-Ling Liu Yu-Rong Tang Liang Wang Source Type: research
