Elevated angiotensin II induces platelet apoptosis through promoting oxidative stress in an AT1R-dependent manner during sepsis
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.16382. Online ahead of print.ABSTRACTThrombocytopenia is independently related with increased mortality in severe septic patients. Renin-angiotensin system (RAS) is elevated in septic subjects; accumulating studies show that angiotensin II (Ang II) stimulate the intrinsic apoptosis pathway by promoting reactive oxygen species (ROS) production. However, the mechanisms underlying the relationship of platelet apoptosis and RAS system in sepsis have not been fully elucidated. The present study aimed to elucidate whether the RAS was involved in the pathogenesis of sepsis-associated...
Source: J Cell Mol Med - February 24, 2021 Category: Molecular Biology Authors: Dun-Feng Xu Yu-Jian Liu Yan-Fei Mao Yan Wang Chu-Fan Xu Xiao-Yan Zhu Lai Jiang Source Type: research

TTN/OBSCN 'Double-Hit' predicts favourable prognosis, 'immune-hot' subtype and potentially better immunotherapeutic efficacy in colorectal cancer
This study revealed CRC patients with TTN/OBSCN 'Double-Hit' was significantly associated favourable prognosis, 'immune-hot' subtype and potentially better immunotherapeutic efficacy.PMID:33624434 | DOI:10.1111/jcmm.16393 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 24, 2021 Category: Molecular Biology Authors: Zaoqu Liu Libo Wang Chunguang Guo Long Liu Dechao Jiao Zhenqiang Sun Kunpeng Wu Yanan Zhao Xinwei Han Source Type: research

Tumour angiogenesis normalized by myo-inositol trispyrophosphate alleviates hypoxia in the microenvironment and promotes antitumor immune response
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.16399. Online ahead of print.ABSTRACTPathologic angiogenesis directly responds to tumour hypoxia and controls the molecular/cellular composition of the tumour microenvironment, increasing both immune tolerance and stromal cooperation with tumour growth. Myo-inositol-trispyrophosphate (ITPP) provides a means to achieve stable normalization of angiogenesis. ITPP increases intratumour oxygen tension (pO2 ) and stabilizes vessel normalization through activation of endothelial Phosphatase-and-Tensin-homologue (PTEN). Here, we show that the tumour reduction due to the ITPP-induced m...
Source: J Cell Mol Med - February 24, 2021 Category: Molecular Biology Authors: Bouchra El Hafny-Rahbi Klaudia Brodaczewska Guillaume Collet Aleksandra Majewska Krzysztof Klimkiewicz Anthony Delalande Catherine Grillon Claudine Kieda Source Type: research

Depressed β-adrenergic inotropic responsiveness and intracellular calcium handling abnormalities in Duchenne Muscular Dystrophy patients' induced pluripotent stem cell-derived cardiomyocytes
J Cell Mol Med. 2021 Feb 22. doi: 10.1111/jcmm.16341. Online ahead of print.ABSTRACTDuchenne muscular dystrophy (DMD), caused by mutations in the dystrophin gene, is an X-linked disease affecting male and rarely adult heterozygous females, resulting in death by the late 20s to early 30s. Previous studies reported depressed left ventricular function in DMD patients which may result from deranged intracellular Ca2+ -handling. To decipher the mechanism(s) underlying the depressed LV function, we tested the hypothesis that iPSC-CMs generated from DMD patients feature blunted positive inotropic response to β-adrenergic sti...
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Lucy N Mekies Danielle Regev Binyamin Eisen Jonatan Fernandez-Gracia Polina Baskin Ronen Ben Jehuda Rita Shulman Irina Reiter Raz Palty Michael Arad Eyal Gottlieb Ofer Binah Source Type: research

Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR-433-3p/ IGF1R axis
In conclusion, it was demonstrated that circ_0002137 could restrain the progression of OS through regulating miR-433-3p/IGF1R axis, providing a comprehensive landscape of circ_0002137 in the generation and development of OS.PMID:33621401 | DOI:10.1111/jcmm.16166 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Meng Zhang Guang-Yang Yu Gang Liu Wei-Dong Liu Source Type: research

Adipose triglyceride lipase promotes the proliferation of colorectal cancer cells via enhancing the lipolytic pathway
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.16349. Online ahead of print.ABSTRACTAbnormal lipid metabolism is the sign of tumour cells. Previous researches have revealed that the lipolytic pathway may contribute to the progression of colorectal cancer (CRC). However, adipose triglyceride lipase (ATGL) role in CRC cells remains unclear. Here, we find that elevated ATGL positively correlates with CRC clinical stages and negatively associates with overall survival. Overexpression of ATGL significantly promotes CRC cell proliferation, while knockdown of ATGL inhibits the proliferation and promotes the apoptosis of CRC cells...
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Haofan Yin Wentao Li Laiming Mo Shaotuan Deng Weijia Lin Caiqi Ma Zhaofan Luo Chuanghua Luo Honghai Hong Source Type: research

Isoflurane post-conditioning contributes to anti-apoptotic effect after cerebral ischaemia in rats through the ERK5/MEF2D signaling pathway
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.16282. Online ahead of print.ABSTRACTThe mechanisms of brain protection during ischaemic reperfusion injury induced by isoflurane (ISO) post-conditioning are unclear. Myocyte enhancement factor 2 (MEF2D) has been shown to promote neural survival in a variety of models, in which multiple survival and death signals converge on MEF2D and modulate its activity. Here, we investigated the effect of MEF2D on the neuroprotective effects of ISO post-conditioning on rats after cerebral ischaemia/reperfusion (I/R) injury. Rats underwent middle cerebral artery occlusion (MCAO) surgery wit...
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Qingtong Zhang Jiangwen Yin Feng Xu Jingwen Zhai Jieting Yin Mingyue Ge Wenyi Zhou Nian Li Xinlei Qin Yan Li Sheng Wang Source Type: research

Cancer-associated fibroblast-derived exosomal microRNA-24-3p enhances colon cancer cell resistance to MTX by down-regulating CDX2/HEPH axis
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.15765. Online ahead of print.ABSTRACTMicroRNA-24-3p (miR-24-3p) has been implicated as a key promoter of chemotherapy resistance in numerous cancers. Meanwhile, cancer-associated fibroblasts (CAFs) can secret exosomes to transfer miRNAs, which mediate tumour development. However, little is known regarding the molecular mechanism of CAF-derived exosomal miR-24-3p in colon cancer (CC). Hence, this study intended to characterize the functional relevance of CAF-derived exosomal miR-24-3p in CC cell resistance to methotrexate (MTX). We identified differentially expressed HEPH, CDX2...
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Hong-Wei Zhang Yi Shi Ji-Bin Liu Hui-Min Wang Pei-Yao Wang Zhi-Jun Wu Liu Li Li-Peng Gu Ping-Sheng Cao Gao-Ren Wang Yu-Shui Ma Da Fu Source Type: research

KDM5A silencing transcriptionally suppresses the FXYD3-PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR-433 up-regulation
J Cell Mol Med. 2021 Feb 23. doi: 10.1111/jcmm.16371. Online ahead of print.ABSTRACTHepatocellular cancer (HCC) has been reported to belong to one of the highly vascularized solid tumours accompanied with angiogenesis of human umbilical vein endothelial cells (HUVECs). KDM5A, an attractive drug target, plays a critical role in diverse physiological processes. Thus, this study aims to investigate its role in angiogenesis and underlying mechanisms in HCC. ChIP-qPCR was utilized to validate enrichment of H3K4me3 and KDM5A on the promotor region of miR-433, while dual luciferase assay was carried out to confirm the targeting r...
Source: J Cell Mol Med - February 23, 2021 Category: Molecular Biology Authors: Yu-Shui Ma Ting-Miao Wu Bin Qian Yu-Shan Liu Hua Ding Ming-Ming Fan Ji-Bin Liu Fei Yu Hui-Min Wang Yi Shi Li-Peng Gu Liu Li Lin-Lin Tian Pei-Yao Wang Gao-Ren Wang Zhi-Jun Wu Qi-Fei Zou Chang-Chun Ling Da Fu Source Type: research

Circular RNA  circEIF4G2 aggravates renal fibrosis in diabetic nephropathy by sponging miR-218
J Cell Mol Med. 2020 Dec 7. doi: 10.1111/jcmm.16129. Online ahead of print.ABSTRACTCircular RNAs play essential roles in the development of various human diseases. However, how circRNAs are involved in diabetic nephropathy (DN) are not fully understood. Our study aimed to investigate the effects of circRNA circEIF4G2 on DN. Experiments were performed in the db/db mouse model of type 2 diabetes and NRK-52E cells. We found that circEIF4G2 was significantly up-regulated in the kidneys of db/db mice and NRK-52E cells stimulated by high glucose. circEIF4G2 knockdown inhibited the expressions of TGF-β1, Collagen I and Fibro...
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Authors: Bojin Xu Qianqian Wang Wenyi Li Lili Xia Xiaoxu Ge Lisha Shen Zhen Cang Wenfang Peng Kan Shao Shan Huang Source Type: research

Corrigendum
J Cell Mol Med. 2020 Dec;24(24):14650-14651. doi: 10.1111/jcmm.15893.NO ABSTRACTPMID:33615689 | PMC:PMC7754053 | DOI:10.1111/jcmm.15893 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Source Type: research

Corrigendum
J Cell Mol Med. 2020 Dec;24(23):14121. doi: 10.1111/jcmm.16042.NO ABSTRACTPMID:33615699 | PMC:PMC7788450 | DOI:10.1111/jcmm.16042 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Source Type: research

Erratum
J Cell Mol Med. 2020 Dec;24(23):14122-14124. doi: 10.1111/jcmm.16112.NO ABSTRACTPMID:33615700 | PMC:PMC7754011 | DOI:10.1111/jcmm.16112 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Source Type: research

A novel hypoxia gene signature indicates prognosis and immune microenvironments characters in patients with hepatocellular carcinoma
In conclusion, we identified a novel HCC-suitable 21-gene hypoxia signature that could be used to estimate the hypoxia exposure in HCC tissues and indicated prognosis and a series of important clinical features in HCCs. It may enable the development of personalized counselling or treatment strategies for HCC patients with different levels of hypoxia exposure.PMID:33616276 | DOI:10.1111/jcmm.16249 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Authors: Qiangnu Zhang Lijun Qiao Juan Liao Quan Liu Pengyu Liu Liping Liu Source Type: research

A novel hypoxia gene signature indicates prognosis and immune microenvironments characters in patients with hepatocellular carcinoma.
In conclusion, we identified a novel HCC-suitable 21-gene hypoxia signature that could be used to estimate the hypoxia exposure in HCC tissues and indicated prognosis and a series of important clinical features in HCCs. It may enable the development of personalized counselling or treatment strategies for HCC patients with different levels of hypoxia exposure. PMID: 33616276 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 22, 2021 Category: Molecular Biology Authors: Zhang Q, Qiao L, Liao J, Liu Q, Liu P, Liu L Tags: J Cell Mol Med Source Type: research

Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells.
Abstract Ferroptosis is an iron-dependent mode of non-apoptotic cell death characterized by accumulation of lipid reactive oxygen species (ROS). As a regulator of ROS, cytoglobin (CYGB) plays an important role in oxygen homeostasis and acts as a tumour suppressor. However, the mechanism by which CYGB regulates cell death is largely unknown. Here, we show that CYGB overexpression increased ROS accumulation and disrupted mitochondrial function as determined by the oxygen consumption rate and membrane potential. Importantly, ferroptotic features with accumulated lipid ROS and malondialdehyde were observed in CYGB-ove...
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Ye S, Xu M, Zhu T, Chen J, Shi S, Jiang H, Zheng Q, Liao Q, Ding X, Xi Y Tags: J Cell Mol Med Source Type: research

Identification of rare variants causing urea cycle disorders: A clinical, genetic, and biophysical study.
In conclusion, this study expanded the genetic variation spectrum of UCD, gave solid evidence for counselling to the affected families, and should facilitate the functional study on the proteins in urea cycle. PMID: 33611823 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Liu F, Bao LS, Liang RJ, Zhao XY, Li Z, Du ZF, Lv SG Tags: J Cell Mol Med Source Type: research

MARCH5 restores endothelial cell function against ischaemic/hypoxia injury via Akt/eNOS pathway.
In conclusion, these results indicated that MARCH5 acts as a protective factor in ischaemia/hypoxia-induced ECs injury partially through Akt/eNOS pathway. PMID: 33611830 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Lei W, Li J, Li C, Chen L, Huang F, Xiao D, Zhang J, Zhao J, Li G, Qu T, Zhou H, Liao Y, Chen M Tags: J Cell Mol Med Source Type: research

Metformin attenuates post-epidural fibrosis by inhibiting the TGF- β1/Smad3 and HMGB1/TLR4 signaling pathways.
Metformin attenuates post-epidural fibrosis by inhibiting the TGF-β1/Smad3 and HMGB1/TLR4 signaling pathways. J Cell Mol Med. 2021 Feb 21;: Authors: Song Z, Wu T, Sun J, Wang H, Hua F, Nicolas YSM, Kc R, Chen K, Jin Z, Liu J, Zhang M Abstract Excessive post-epidural fibrosis is a common cause of recurrent back pain after spinal surgery. Though various treatment methods have been conducted, the safe and effective drug for alleviating post-epidural fibrosis remains largely unknown. Metformin, a medicine used in the treatment of type 2 diabetes, has been noted to relieve fibrosis in various organs. ...
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Song Z, Wu T, Sun J, Wang H, Hua F, Nicolas YSM, Kc R, Chen K, Jin Z, Liu J, Zhang M Tags: J Cell Mol Med Source Type: research

FXR-mediated epigenetic regulation of GLP-1R expression contributes to enhanced incretin effect in diabetes after RYGB.
In this study, we investigated how Roux-en-Y gastric bypass (RYGB) enhances glucagon-like peptide 1 (GLP-1) response in GK rats and explored the potential link between RYGB-stimulated BAs/FXR signalling and GLP-1R-linked signalling in β-cells, a key pathway that regulates glucose-stimulated insulin secretion (GSIS). Here we show that RYGB restores GLP-1R expression in GK rat islets. This involves increased total BAs as well as chenodeoxycholic acid (CDCA), leading to FXR activation, increasing FXR binding to the promoter of Glp-1r and enhancing occupancy of histone acetyltransferase steroid receptor coactivator-1 (SRC...
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Kong X, Feng L, Yan D, Li B, Yang Y, Ma X Tags: J Cell Mol Med Source Type: research

Characterization of an endoplasmic reticulum stress-related signature to evaluate immune features and predict prognosis in glioma.
In conclusion, we present an ER stress risk model that is an independent prognostic factor and indicates the general immune characteristics in the glioma microenvironment. PMID: 33611848 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Zhang Q, Guan G, Cheng P, Cheng W, Yang L, Wu A Tags: J Cell Mol Med Source Type: research

MicroRNA-130a inhibits proliferation of vascular smooth muscle cells by suppressing autophagy via ATG2B.
In conclusion, our data demonstrate that miR-130a inhibits VSMCs proliferation via ATG2B, which indicates that miR-130a could be a potential therapeutic target that regulates autophagy in atherosclerosis obliterans. PMID: 33611856 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 21, 2021 Category: Molecular Biology Authors: Zheng L, Wang Z, Li Z, Wang M, Wang W, Chang G Tags: J Cell Mol Med Source Type: research

Lenvatinib prevents liver fibrosis by inhibiting hepatic stellate cell activation and sinusoidal capillarization in experimental liver fibrosis.
Abstract Molecular targeted agents are pharmacologically used to treat liver fibrosis and have gained increased attention. The present study examined the preventive effect of lenvatinib on experimental liver fibrosis and sinusoidal capillarization as well as the in vitro phenotypes of hepatic stellate cells. LX-2, a human stellate cell line, was used for in vitro studies. In vivo liver fibrosis was induced in F344 rats using carbon tetrachloride by intraperitoneal injection for 8 weeks, and oral administration of lenvatinib was started two weeks after initial injection of carbon tetrachloride. Lenvatinib rest...
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Ogawa H, Kaji K, Nishimura N, Takagi H, Ishida K, Takaya H, Kawaratani H, Moriya K, Namisaki T, Akahane T, Yoshiji H Tags: J Cell Mol Med Source Type: research

Prostate adenocarcinoma and COVID-19: The possible impacts of TMPRSS2 expressions in susceptibility to SARS-CoV-2.
In this study, we conducted the expression profiles of the TMPRSS2 gene for COVID-19 in different normal tissues and PRAD (prostate adenocarcinoma) tumour tissues. TMPRSS2 is highly expressed in normal tissues including the small intestine, prostate, pancreas, salivary gland, colon, stomach, seminal vesicle and lung, and is increased in PRAD tissues, indicating that SARS-CoV-2 might attack not only the lungs and other normal organs, but also in PRAD cancer tissues. Hypomethylation of TMPRSS2 promoter may not be the mechanism for TMPRSS2 overexpression in PRAD tissues and PRAD pathogenesis. TMPRSS2 expresses eleven isoforms...
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Cheng J, Zhou J, Fu S, Fu J, Zhou B, Chen H, Fu J, Wei C Tags: J Cell Mol Med Source Type: research

AS602801 sensitizes glioma cells to temozolomide and vincristine by blocking gap junction communication between glioma cells and astrocytes.
In this study, we aimed to investigate the combined chemotherapeutic effect of AS602801 and TMZ/ VCR in glioma cells both. Dye transfer assay was used to evaluate the gap junction activity between U251 cells and astrocytes. Western blot and immunohistochemistry were carried out to analyse the expression of p-JNK, CX43 and CASP-3 proteins treated under different conditions. AS602801 significantly suppressed the gap junction activity between U251 cells and astrocytes. The expression of p-JNK and CX43 was remarkably inhibited by AS602801. TMZ/VCR-induced apoptosis of glioma cells was effectively enhanced by AS602801 treatment...
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Zhang S, Gong Y, Wang H, Li Z, Huang Y, Fu X, Xiang P, Fan T Tags: J Cell Mol Med Source Type: research

The aberrant expression in epithelial cells of the mesenchymal isoform of FGFR2 controls the negative crosstalk between EMT and autophagy.
Abstract Signalling of the epithelial splicing variant of fibroblast growth factor receptor 2 (FGFR2b) triggers both differentiation and autophagy, while the aberrant expression of the mesenchymal FGFR2c isoform in epithelial cells induces impaired differentiation, inhibition of autophagy as well as the induction of the epithelial-mesenchymal transition (EMT). In light of the widely proposed negative loop linking autophagy and EMT in the early steps of carcinogenesis, here we investigated the possible involvement of FGFR2c aberrant expression and signalling in orchestrating this crosstalk in human keratinocytes. B...
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Ranieri D, Nanni M, Guttieri L, Torrisi MR, Belleudi F Tags: J Cell Mol Med Source Type: research

Diagnostic and prognostic value of CSF neurofilaments in a cohort of patients with motor neuron disease: A cross-sectional study.
In this study, we described our cohort of ALS patients in order to investigate whether and how cerebrospinal fluid (CSF) p-NfH and NfL levels may reflect progression rate, MN involvement and the extent of neurodegeneration. CSF p-NfH and NfL were significantly increased in ALS compared with healthy and disease controls, including patients with other forms of MND, and were higher in patients with more aggressive disease course, reflecting progression rate. We also evaluated neurofilament diagnostic accuracy in our centre, identifying with high sensitivity and 100% specificity cut-off values of 0.652 ng/mL for CSF p-NfH (P 
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Gagliardi D, Faravelli I, Meneri M, Saccomanno D, Govoni A, Magri F, Ricci G, Siciliano G, Pietro Comi G, Corti S Tags: J Cell Mol Med Source Type: research

1 ɑ,25-Dihydroxyvitamin D3 promotes osteogenesis by down-regulating FGF23 in diabetic mice.
In this study, we investigated the effect of 1,25D on FGF23 under diabetic condition both in vitro and in vivo. The results showed that 1,25D down-regulated the expression of FGF23 in osteoblast significantly though a dose-dependent manner in vitro within high glucose environment. Western blot and immunofluorescence analysis indicated that 1,25D activated PI3K/Akt signalling through binding to vitamin D receptor (VDR), which inhibited the phosphorylation of the transcription factor Forkhead Box O1 (FOXO1). Decreased phosphorylation of FOXO1 down-regulated the expression Dickkopf-1 (DKK1), a well-known inhibitor of Wnt sign...
Source: J Cell Mol Med - February 20, 2021 Category: Molecular Biology Authors: Luo W, Jiang Y, Yi Z, Wu Y, Gong P, Xiong Y Tags: J Cell Mol Med Source Type: research

STAT1 is a key gene in a gene regulatory network related to immune phenotypes in bladder cancer: An integrative analysis of multi-omics data.
In conclusion, the present study addressed a field synopsis between genetic and epigenetic events in immune phenotypes of bladder cancer. PMID: 33608980 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 19, 2021 Category: Molecular Biology Authors: Weng H, Yuan S, Huang Q, Zeng XT, Wang XH Tags: J Cell Mol Med Source Type: research

Sertoli cell-derived exosomal MicroRNA-486-5p regulates differentiation of spermatogonial stem cell through PTEN in mice.
Abstract Self-renewal and differentiation of spermatogonial stem cell (SSC) are critical for male fertility and reproduction, both of which are highly regulated by testicular microenvironment. Exosomal miRNAs have emerged as new components in intercellular communication. However, their roles in the differentiation of SSC remain unclear. Here, we observed miR-486-5p enriched in Sertoli cell and Sertoli cell-derived exosomes. The exosomes mediate the transfer of miR-486-5p from Sertoli cells to SSCs. Exosomes release miR-486-5p, thus up-regulate expression of Stra8 (stimulated by retinoic acid 8) and promote differe...
Source: J Cell Mol Med - February 19, 2021 Category: Molecular Biology Authors: Li Q, Li H, Liang J, Mei J, Cao Z, Zhang L, Luo J, Tang Y, Huang R, Xia H, Zhang Q, Xiang Q, Yang Y, Huang Y Tags: J Cell Mol Med Source Type: research

Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice.
In conclusion, the data suggested that the co-activation of TLR2 and NOD2 could induce significant synergistic radioprotective effects and CL429 might be a potential high-efficiency selective agent. PMID: 33609010 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 19, 2021 Category: Molecular Biology Authors: Cheng Y, Du J, Liu R, Dong S, Cai J, Gao F, Liu C Tags: J Cell Mol Med Source Type: research

Cell-specific network analysis of human folliculogenesis reveals network rewiring in antral stage oocytes.
Abstract Although previous studies have explored the gene expression profiles of human oocytes and granulosa cells by single-cell RNA sequencing (scRNA-seq), the dynamic regulatory network at a single-cell resolution during folliculogenesis remains largely unknown. We identified 10 functional modules by WGCNA, four of which were significantly correlated with primary/antral oocyte and antral/pre-ovulatory granulosa cells. Functional enrichment analysis showed that the brown module, which was correlated with antral oocyte, was enriched in oocyte differentiation, and two core subnetworks identified by MCODE were invo...
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Wang S, Gong Y, Wang Z, Greenbaum J, Xiao HM, Deng HW Tags: J Cell Mol Med Source Type: research

The functions of autophagy at the tumour-immune interface.
Abstract Autophagy is frequently induced in the hypoxic tumour microenvironment. Accumulating evidence reveals important functions of autophagy at the tumour-immune interface. Herein, we propose an update on the roles of autophagy in modulating tumour immunity. Autophagy promotes adaptive resistance of established tumours to the cytotoxic effects of natural killer cells (NKs), macrophages and effector T cells. Increased autophagic flux in tumours dampen their immunogenicity and inhibits the expansion of cytotoxic T lymphocytes (CTLs) by suppressing the activation of STING type I interferon signalling (IFN-I) innat...
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Luo X, Qiu Y, Dinesh P, Gong W, Jiang L, Feng X, Li J, Jiang Y, Lei YL, Chen Q Tags: J Cell Mol Med Source Type: research

BMP9-initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/ β-catenin signalling activity.
BMP9-initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/β-catenin signalling activity. J Cell Mol Med. 2021 Feb 18;: Authors: Luo W, Zhang L, Huang B, Zhang H, Zhang Y, Zhang F, Liang P, Chen Q, Cheng Q, Tan D, Tan Y, Song J, Zhao T, Haydon RC, Reid RR, Luu HH, Lee MJ, El Dafrawy M, Ji P, He TC, Gou L Abstract Teeth arise from the tooth germ through sequential and reciprocal interactions between immature epithelium and mesenchyme during development. However, the detailed mechanism underlying tooth development from tooth germ mesenchymal cel...
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Luo W, Zhang L, Huang B, Zhang H, Zhang Y, Zhang F, Liang P, Chen Q, Cheng Q, Tan D, Tan Y, Song J, Zhao T, Haydon RC, Reid RR, Luu HH, Lee MJ, El Dafrawy M, Ji P, He TC, Gou L Tags: J Cell Mol Med Source Type: research

Serum macrophage inhibitory cytokine-1 as a clinical marker for non-small cell lung cancer.
Abstract The aim of this study was to investigate the value of serum macrophage inhibitory factor-1 (MIC-1) level in patients with non-small cell lung cancer (NSCLC). Serum samples from 296 patients with NSCLC and 240 healthy controls were collected. The levels of serum MIC-1 were determined by ELISA. The serum MIC-1 levels in NSCLC patients were higher than that of the controls (P 
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Xu C, Li L, Wang W, Zhang Q, Zhang X, Yang R Tags: J Cell Mol Med Source Type: research

Cyclic stretching boosts microRNA-499 to regulate Bcl-2 via microRNA-208a in atrial fibroblasts.
In conclusion, miR-208a mediated the regulation of miR-499 on Bcl-2 expression in stretched HCF-aa and hearts with volume overload. PMID: 33605072 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Chua SK, Wang BW, Yu YJ, Fang WJ, Lin CM, Shyu KG Tags: J Cell Mol Med Source Type: research

Lansoprazole promotes cisplatin-induced acute kidney injury via enhancing tubular necroptosis.
Abstract Acute kidney injury (AKI) is the main obstacle that limits the use of cisplatin in cancer treatment. Proton pump inhibitors (PPIs), the most commonly used class of medications for gastrointestinal complications in cancer patients, have been reported to cause adverse renal events. However, the effect of PPIs on cisplatin-induced AKI remains unclear. Herein, the effect and mechanism of lansoprazole (LPZ), one of the most frequently prescribed PPIs, on cisplatin-induced AKI were investigated in vivo and in vitro. C57BL/6 mice received a single intraperitoneal (i.p.) injection of cisplatin (18 mg/kg) to ...
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: Ye L, Pang W, Huang Y, Wu H, Huang X, Liu J, Wang S, Yang C, Pan Q, Liu H Tags: J Cell Mol Med Source Type: research

P62-positive aggregates are homogenously distributed in the myocardium and associated with the type of mutation in genetic cardiomyopathy.
The objective of this study was to investigate the distribution in the myocardium of such aggregates in relation to specific pathogenic genetic mutations in cardiomyopathy hearts. Hearts from 32 genetic cardiomyopathy patients, 4 non-genetic cardiomyopathy patients and 5 controls were studied. Microscopic slices from an entire midventricular heart slice were stained for p62 (sequestosome-1, marker for aggregated proteins destined for autophagy). The percentage of cardiomyocytes with p62 accumulation was higher in cardiomyopathy hearts (median 3.3%) than in healthy controls (0.3%; P 
Source: J Cell Mol Med - February 18, 2021 Category: Molecular Biology Authors: van der Klooster ZJ, Sepehrkhouy S, Dooijes D, Te Rijdt WP, Schuiringa FSAM, Lingeman J, van Tintelen JP, Harakalova M, Goldschmeding R, Suurmeijer AJH, Asselbergs FW, Vink A Tags: J Cell Mol Med Source Type: research

Bortezomib suppresses self-renewal and leukemogenesis of leukemia stem cell by NF- ĸB-dependent inhibition of CDK6 in MLL-rearranged myeloid leukemia.
In conclusion, our results suggest that bort selectively targets LSC in MLL rearrangements. Bort might be a prospective drug for AML patients bearing MLL rearrangements. PMID: 33599085 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 17, 2021 Category: Molecular Biology Authors: Zhou B, Qin Y, Zhou J, Ruan J, Xiong F, Dong J, Huang X, Yu Z, Gao S Tags: J Cell Mol Med Source Type: research

Low expression of GSTP1 in the aqueous humour of patients with primary open-angle glaucoma.
Abstract Primary open-angle glaucoma (POAG) is characterized by irreversible neurodegeneration accompanied by visual field defects and high intraocular pressure. Currently, an effective treatment is not available to prevent the progression of POAG, other than treatments to decrease the high intraocular pressure. We performed proteomic analysis of aqueous humour (AH) samples from patients with POAG combined with cataract and patients with cataract to obtain a better understanding of the pathogenesis of POAG and explore potential treatment targets for this condition. Samples were collected from 10 patients with POAG...
Source: J Cell Mol Med - February 17, 2021 Category: Molecular Biology Authors: Liu A, Wang L, Feng Q, Zhang D, Chen K, Yiming GH, Wang Q, Hong Y, Whelchel A, Zhang X, Li X, Dong L Tags: J Cell Mol Med Source Type: research

FGF21 promotes ischaemic angiogenesis and endothelial progenitor cells function under diabetic conditions in an AMPK/NAD+-dependent manner.
Abstract Diabetic vascular complications are closely associated with long-term vascular dysfunction and poor neovascularization. Endothelial progenitor cells (EPCs) play pivotal roles in maintaining vascular homeostasis and triggering angiogenesis, and EPC dysfunction contributes to defective angiogenesis and resultant diabetic vascular complications. Fibroblast growth factor 21 (FGF21) has received substantial attention as a potential therapeutic agent for diabetes via regulating glucose and lipid metabolism. However, the effects of FGF21 on diabetic vascular complications remain unclear. In the present study, th...
Source: J Cell Mol Med - February 17, 2021 Category: Molecular Biology Authors: Dai Q, Fan X, Meng X, Sun S, Su Y, Ling X, Chen X, Wang K, Dai X, Zhang C, Da S, Zhang G, Gu C, Chen H, He J, Hu H, Yu L, Pan X, Tan Y, Yan X Tags: J Cell Mol Med Source Type: research

Role of sphingosine-1-phosphate receptors in vascular injury of inflammatory bowel disease.
Abstract Sphingosine-1-phosphate receptors (S1PRs) have an impact on the intestinal inflammation of inflammatory bowel disease (IBD) by regulating lymphocyte migration and differentiation. S1PR modulators as an emerging therapeutic approach are being investigated for the treatment of IBD. However, the role of S1PRs in intestinal vessels has not drawn much attention. Intestinal vascular damage is one of the major pathophysiological features of IBD, characterized by increased vascular density and impaired barrier function. S1PRs have pleiotropic effects on vascular endothelial cells, including proliferation, migrati...
Source: J Cell Mol Med - February 17, 2021 Category: Molecular Biology Authors: Wang X, Chen S, Xiang H, Liang Z, Lu H Tags: J Cell Mol Med Source Type: research

Ferroptosis-related gene signature as a prognostic marker for lower-grade gliomas.
In this study, the prognostic value of ferroptosis-related genes was investigated in lower-grade gliomas (LGG). We downloaded the ferroptosis-related genes from the FerrDb dataset. Univariate Cox and LASSO regression analyses were applied to identify genes correlated with overall survival (OS). Subsequently, 12 ferroptosis-related genes were screened to establish the prognostic signature using stepwise multivariate Cox regression. According to the median value of risk scores, patients were divided into low- and high-risk subgroups. The Kaplan-Meier curves showed the high-risk group had a lower OS. The predictive power...
Source: J Cell Mol Med - February 16, 2021 Category: Molecular Biology Authors: Zheng Y, Ji Q, Xie L, Wang C, Yu CN, Wang YL, Jiang J, Chen F, Li WB Tags: J Cell Mol Med Source Type: research

Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity.
Abstract The aim of this study was to investigate how mesenchymal stromal cells (MSCs) modulate metabolic balance and attenuate hepatic lipotoxicity in the context of non-alcoholic fatty liver disease (NAFLD). In vivo, male SD rats were fed with high-fat diet (HFD) to develop NAFLD; then, they were treated twice by intravenous injections of rat bone marrow MSCs. In vitro, HepG2 cells were cocultured with MSCs by transwell and exposed to palmitic acid (PA) for 24 hours. The endoplasmic reticulum (ER) stressor thapsigargin and sarco/ER Ca2+ -ATPase (SERCA2)-specific siRNA were used to explore the regulation of ...
Source: J Cell Mol Med - February 16, 2021 Category: Molecular Biology Authors: Li L, Zeng X, Liu Z, Chen X, Li L, Luo R, Liu X, Zhang J, Liu J, Lu Y, Cheng J, Chen Y Tags: J Cell Mol Med Source Type: research

Red cell distribution width and erythrocyte osmotic stability in type 2 diabetes mellitus.
This study aimed to investigate the relationship between red cell distribution width (RDW) and erythrocyte osmotic stability in non-diabetic and diabetic individuals in both sexes. The study sample (N = 122) was constituted by 53 type 2 diabetics (DM) and 69 non-diabetics (ND), being 21 and 22 men in each group, respectively. The osmotic stability of erythrocytes was obtained by the variation in saline concentration (dX) capable of determining hypoosmotic lysis. Higher RDW values and lower serum iron concentrations were found in the diabetic group when compared to the non-diabetic volunteers. In the group of diab...
Source: J Cell Mol Med - February 16, 2021 Category: Molecular Biology Authors: Knychala MA, Garrote-Filho MDS, Batista da Silva B, Neves de Oliveira S, Yasminy Luz S, Marques Rodrigues MO, Penha-Silva N Tags: J Cell Mol Med Source Type: research

Study of oral microorganisms contributing to non-carious cervical lesions via bacterial interaction and pH regulation.
In conclusion, microorganisms and NCCLs were confirmed directly related through adherent bacterial interactions and pH regulation. The research provides a new perspective and experimental evidence for the relation between microorganisms and NCCLs, which guides clinical treatment and preventive dentistry in the future. PMID: 33591640 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 16, 2021 Category: Molecular Biology Authors: Huang X, She L, Liu H, Liu P, Chen J, Chen Y, Zhou W, Lu Y, Lin J Tags: J Cell Mol Med Source Type: research

Myocarditis and heart function impairment occur in neonatal mice following in utero exposure to the Zika virus.
PMID: 33590650 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 15, 2021 Category: Molecular Biology Authors: Bai C, Hao J, Li S, Gao GF, Nie Y, Han P Tags: J Cell Mol Med Source Type: research

Extracellular vesicle-mediated regulation of tumor angiogenesis- implications for anti-angiogenesis therapy.
Abstract Angiogenesis plays an important role in tumour progression. However, anti-angiogenesis therapy of inhibiting pro-angiogenic factors failed to meet expectations in certain types of tumour in clinical trials. Recent studies reveal that tumour-derived extracellular vesicles (EVs) are essential in tumour angiogenesis and anti-angiogenesis drug resistance. This function has most commonly been attributed to EV contents including proteins and non-coding RNAs. Here, we summarize the recent findings of tumour-derived EV contents associated with regulating angiogenesis and illustrate the underlying mechanisms. In a...
Source: J Cell Mol Med - February 14, 2021 Category: Molecular Biology Authors: Zhang S, Yang J, Shen L Tags: J Cell Mol Med Source Type: research

EphA2 promotes tumorigenicity of cervical cancer by up-regulating CDK6.
Abstract Erythropoietin-producing hepatocellular receptor A2 (EphA2) receptor tyrosine kinase plays an important role in tissue organization and homeostasis in normal organs. EphA2 is overexpressed in a variety of types of solid tumours with oncogenic functions. However, the role of EphA2 in cervical cancer (CC) is still needed to be further explored. Here, we examined the role of EphA2 by establishing a stable EphA2 knock-down CC cell lines or a stable EphA2-overexpressed CC cells lines. Overexpression of EphA2 increased cell proliferation and migration of CC while EphA2 knock-down decreased the CC tumorigenicity...
Source: J Cell Mol Med - February 14, 2021 Category: Molecular Biology Authors: Huang C, Chen Z, He Y, He Z, Ban Z, Zhu Y, Ding L, Yang C, Jeong JH, Yuan W, Yang L Tags: J Cell Mol Med Source Type: research

A literature review on function and regulation mechanism of DKK4.
Abstract Dickkopf-related protein 4 (DKK4) is a member of the dickkopf family and an inhibitor of the Wnt/β-catenin signalling pathway. This review surveyed the single nucleotide polymorphisms (SNPs), copy number variations (CNVs), hypermethylation, regulation mechanism, correlation with clinicopathological parameters and chemotherapeutic resistance of DKK4. The signal pathways involved in DKK4 mainly include Wnt/β-catenin pathway and Wnt-JNK pathway independent β-catenin. DKK4 expression was upregulated in Renal Cell Carcinoma (RCC), Colorectal Cancer, Gastric Cancer (GC), Non-small Cell Lung Cance...
Source: J Cell Mol Med - February 14, 2021 Category: Molecular Biology Authors: Lou X, Meng Y, Hou Y Tags: J Cell Mol Med Source Type: research