Retraction: Ectopic expression of ROR1 prevents cochlear hair cell loss in guinea pigs with noise-induced hearing loss
J Cell Mol Med. 2024 Feb;28(4):e18169. doi: 10.1111/jcmm.18169.NO ABSTRACTPMID:38339763 | PMC:PMC10863977 | DOI:10.1111/jcmm.18169 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 10, 2024 Category: Molecular Biology Source Type: research

Methyltransferase-like 3 mediated RNA m < sup > 6 < /sup > A modifications in the reproductive system: Potentials for diagnosis and therapy
J Cell Mol Med. 2024 Feb;28(4):e18128. doi: 10.1111/jcmm.18128.ABSTRACTSeveral studies have highlighted the functional indispensability of methyltransferase-like 3 (METTL3) in the reproductive system. However, a review that comprehensively interprets these studies and elucidates their relationships is lacking. Therefore, the present work aimed to review studies that have investigated the functions of METTL3 in the reproductive system (including spermatogenesis, follicle development, gametogenesis, reproductive cancer, asthenozoospermia and assisted reproduction failure). This review suggests that METTL3 functions not only ...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Xiaojuan Su Ruifeng Lu Yi Qu Dezhi Mu Source Type: research

Tension regulates the cartilage phenotypic expression of endplate chondrocytes through the α-catenin/actin skeleton/Hippo pathway
J Cell Mol Med. 2024 Feb;28(4):e18133. doi: 10.1111/jcmm.18133.ABSTRACTThe study aimed to investigate the regulatory mechanism of intracellular tension signaling in endplate chondrocytes and its impact on extracellular matrix synthesis. Human endplate chondrocytes were subjected to tension load using Flexcell FX-5000™, and changes in phenotype, morphology, and the expression of Hippo signaling pathway and α-Catenin were assessed through various techniques. Through the overexpression of YAP and inhibition of α-Catenin, the study clarified the intracellular tension signaling pathway and its regulation of extracellular ma...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Min Zhang Shouliang Xiong Daokuan Gao Chen Liu Liang Xiao Source Type: research

Temperature-sensitive hydrogel releasing pectolinarin facilitate scarless wound healing
In this study, we successfully developed a temperature-sensitive Pluronic@F-127 hydrogel as a platform for delivering pectolinarin to promote scarless wound healing. The bioactive pectolinarin was released from the hydrogel, effectively enhancing endothelial cell migration, proliferation and the expression of angiogenesis-related genes. Additionally, a concentration of 20 μg/mL of pectolinarin demonstrated remarkable antioxidant ability, capable of counteracting the detrimental effects of reactive oxygen species (ROS). Our results from rat wound healing models demonstrated that the hydrogel accelerated wound healing, prom...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Xiaohang Chen Haoyue Song Kun Song Yuan Zhang Jia Wang Jinjia Hong Qingpeng Xie Jing Zhao Meixian Liu Xing Wang Source Type: research

Livin is protective in UVB-induced skin photodamage by regulating keratinocyte activation and inflammatory responses
J Cell Mol Med. 2024 Feb;28(4):e18124. doi: 10.1111/jcmm.18124.ABSTRACTUVB radiation can lead to skin photodamage, which might arise from keratinocyte (KC) activation. Nuclear factor kappa B (NF-κB) assumes an essential function in the context of UVB-triggered skin photodamage. Initiating the NF-κB cascade leads to the release of inflammatory factors from KCs. Livin can modulate both KC activation and function, yet it remains uncertain whether and how Livin regulates KC activation induced by UVB. To explore the involvement of Livin in UVB-triggered skin photodamage and its impact on skin damage through NF-κB activation....
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Kaijie Wang Xiaolan You Zhenri Qu Delu Che Xianwei Cao Source Type: research

Adenine base editor-based correction of the cardiac pathogenic Lmna c.1621C   & gt;  T mutation in murine hearts
In this study, we harnessed adeno-associated virus (AAV) and a mouse model carrying the cardiomyopathy-causing Lmna c.1621C > T mutation to demonstrate key steps and concerns in designing a cardiac ABE experiment in vivo. We found DeepABE as a reliable deep-learning-based model to predict ABE editing outcomes in the heart. Screening of sgRNAs for a Cas9 mutant with relieved protospacer adjacent motif (PAM) allowed the reduction of bystander editing. The ABE editing efficiency can be significantly enhanced by modifying the TadA and Cas9 variants, which are core components of ABEs. The ABE systems can be delivered into th...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Luzi Yang Zhanzhao Liu Jinhuan Sun Zhan Chen Fei Gao Yuxuan Guo Source Type: research

Systemic inflammation and its relationship with pruritus in early-stage mycosis fungoides
This study provides evidence of enhanced systemic inflammation in early-stage MF patients. Additionally, the correlation between pruritus with mSWAT scores and systemic inflammation parameters suggests a potential link between pruritus and the inflammatory milieu in MF.PMID:38332520 | PMC:PMC10853580 | DOI:10.1111/jcmm.18125 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Berna Solak Rabia Öztaş Kara Source Type: research

Effect of fentanyl and remifentanil on neuron damage and oxidative stress during induction neurotoxicity
J Cell Mol Med. 2024 Feb;28(4):e18118. doi: 10.1111/jcmm.18118.ABSTRACTOpioids can be used for medical and non-medical purposes. Chronic pain such as cancer, as well as the frequent use of such drugs in places such as operating rooms and intensive care units, and in non-medical areas like drug abuse the effects and side effects of these drugs need to be examined in more detail. For this purpose, the effects of fentanyl and remifentanil drugs on neuroinflammation, oxidative stress and cholinesterase metabolism were investigated. Neuron cells (CRL-10742) were used for the evaluation of the toxicity of fentanyl and remifentan...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Ali Taghizadehghalehjoughi Muhammet Emin Naldan Yesim Yeni Sidika Genc Ahmet Hacimuftuoglu Mesut Isik Adem Necip İsmail Bolat Serkan Yildirim Sukru Beydemir Mahmut Baykan Source Type: research

ITGBL1 promotes anoikis resistance and metastasis in human gastric cancer via the AKT/FBLN2 axis
In conclusion, the findings of our study indicate that ITGBL1 may serve as a possible modulator of resistance to anoikis and the metastatic process in GC.PMID:38332530 | PMC:PMC10853594 | DOI:10.1111/jcmm.18113 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Kanger Shen Wei Xia Kun Wang Juntao Li Wei Xu Haoran Liu Kexi Yang Jinghan Zhu Jiayu Wang Qinhua Xi Tongguo Shi Rui Li Source Type: research

Identification of the shared genes in type 2 diabetes mellitus and osteoarthritis and the role of quercetin
This study investigated the underlying comorbidity mechanism between type 2 diabetes mellitus (T2DM) and osteoarthritis (OA), while also assessing the therapeutic potential of quercetin for early intervention and treatment of these two diseases. The shared genes were obtained through GEO2R, limma and weighted gene co-expression network analysis (WGCNA), and validated using clinical databases and the area under the curves (ROC). Functional enrichment analysis was conducted to elucidate the underlying mechanisms of comorbidity between T2DM and OA. The infiltration of immune cells was analysed using the CIBERSORT algorithm in...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Siyuan Song Jiangyi Yu Source Type: research

Nerve growth factor promote osteogenic differentiation of dental pulp stem cells through MEK/ERK signalling pathways
This study aimed to investigate the role of NGF on the osteogenic differentiation of DPSCs in vitro and the underlying mechanisms. DPSCs were cultured in osteogenic differentiation medium containing NGF (50 ng/mL) for 7 days. Then osteogenic-related genes and protein markers were analysed using qRT-PCR and Western blot, respectively. Furthermore, addition of NGF inhibitor and small interfering RNA (siRNA) transfection experiments were used to elucidate the molecular signalling pathway responsible for the process. NGF increased osteogenic differentiation of DPSCs significantly compared with DPSCs cultured in an osteogenic-i...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Chen Cheng Shuai Tang Shuyue Cui Tong Yang Lan Li Mingrui Zhai Fulan Wei Gang Ding Source Type: research

Ginkgetin effectively mitigates collagen and AA-induced platelet activation via PLC γ2 but not cyclic nucleotide-dependent pathway in human
In conclusion, GK's profound impact on platelet activation, achieved through inhibiting PLCγ2-PKC cascade, culminates in the suppression of downstream signaling and, ultimately, the inhibition of platelet aggregation. These findings underscore the promising therapeutic potential of GK in the CVDs.PMID:38334198 | PMC:PMC10853947 | DOI:10.1111/jcmm.18139 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Chih-Wei Hsia Lan-Hsin Shu Ai-Wei Lee Oanh-Thi Tran Chih-Hao Yang Ting-Lin Yen Wei-Chieh Huang Chih-Hsuan Hsia Thanasekaran Jayakumar Kuan-Rau Chiou Joen-Rong Sheu Source Type: research

Anemoside B4 alleviates arthritis pain via suppressing ferroptosis-mediated inflammation
J Cell Mol Med. 2024 Feb;28(4):e18136. doi: 10.1111/jcmm.18136.ABSTRACTChronic pain is the key manifestations of rheumatoid arthritis. Neuroinflammation in the spinal cord drives central sensitization and chronic pain. Ferroptosis has potentially important roles in the occurrence of neuroinflammation and chronic pain. In the current study, mouse model of collagen-induced arthritis was established by intradermal injection of type II collagen in complete Freund's adjuvant (CFA) solution. CFA inducement resulted in swollen paw and ankle, mechanical and spontaneous pain, and impaired motor coordination. The spinal inflammation...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Chenlu Guo Yuanfen Yue Bojun Wang Shaohui Chen Dai Li Fangshou Zhen Ling Liu Haili Zhu Min Xie Source Type: research

The novel TERF2::PDGFRB fusion gene enhances tumorigenesis via PDGFRB/STAT5 signalling pathways and sensitivity to TKI in ph-like ALL
J Cell Mol Med. 2024 Feb;28(3):e18114. doi: 10.1111/jcmm.18114.ABSTRACTPatients with Philadelphia chromosome-like acute lymphoblastic leukaemia (Ph-like ALL) often face a grim prognosis, with PDGFRB gene fusions being commonly detected in this subgroup. Our study has unveiled a newfound fusion gene, TERF2::PDGFRB, and we have found that patients carrying this fusion gene exhibit sensitivity to dasatinib. Ba/F3 cells harbouring the TERF2::PDGFRB fusion display IL-3-independent cell proliferation through activation of the p-PDGFRB and p-STAT5 signalling pathways. These cells exhibit reduced apoptosis and demonstrate sensitiv...
Source: J Cell Mol Med - February 7, 2024 Category: Molecular Biology Authors: Guo-Fa Xu Zhao Zeng Zhi-Bo Zhang Xiao-Mei Zhang Man Wang Qing Xiao Jun Li Xiao-Qing Xie Sanxiu He Hui-Hui Fu Yi Liu Zai-Liang Yang Yu Chen Jie Shi Biao Wang Hui-Ying Qiu Qi Zhou Yao Liu Su-Ning Chen Source Type: research

Endoplasmic reticulum stress regulators exhibit different prognostic, therapeutic and immune landscapes in pancreatic adenocarcinoma
J Cell Mol Med. 2024 Feb 1. doi: 10.1111/jcmm.18092. Online ahead of print.ABSTRACTEndoplasmic reticulum stress (ERS) and unfolded protein response are the critical processes of tumour biology. However, the roles of ERS regulatory genes in pancreatic adenocarcinoma (PAAD) remain elusive. A novel ERS-related risk signature was constructed using the Lasso regression analysis. Its prognostic value, immune effect, metabolic influence, mutational feature and therapeutic correlation were comprehensively analysed through multiple bioinformatic approaches. The biofunctions of KDELR3 and YWHAZ in pancreatic cancer (PC) cells were a...
Source: J Cell Mol Med - February 2, 2024 Category: Molecular Biology Authors: Shanshan Liu Kaini He Longbao Yang Fangshi Xu Xiaoguang Cui Li Qu Xueyi Li Bin-Cheng Ren Source Type: research