The KIR2DL family serves as prognostic biomarkers and correlates with immune infiltrates in acute myeloid leukaemia
J Cell Mol Med. 2024 Apr;28(8):e18256. doi: 10.1111/jcmm.18256.ABSTRACTAcute myeloid leukaemia (AML) is a prevalent haematological malignancy in which various immune and stromal cells in the bone marrow microenvironment have instrumental roles and substantially influence its progression. KIR2DL is a member of the immunoglobulin-like receptor family and a natural killer (NK) cell surface-specific receptor. However, its impact on immune infiltration regarding AML has not been addressed. We aimed to explore molecular markers associated with the immune microenvironment and prognosis of AML with a particular focus on KIR2DL fam...
Source: J Cell Mol Med - March 25, 2024 Category: Molecular Biology Authors: Wenling Liu Mingming Zhu Ganggang Li Yaming Xi Source Type: research
Targeting HOXA11-AS to mitigate prostate cancer via the glycolytic metabolism: In vitro and in vivo
In this study, PCa cell lines were selected to assess the PCa regulatory role of HOXA11-AS overexpression versus silencing in vitro, and tumour xenografts were performed in nude mice to assess tumour suppression by HOXA11-AS silencing in vivo. HOXA11-AS expression was significantly correlated with clinicopathological factors, epithelial-mesenchymal transition (EMT) and glycolysis. Moreover, key genes downstream of HOXA11-AS exhibited good clinical diagnostic properties for PCa. Furthermore, we studied both in vitro and in vivo effects of HOXA11-AS expression on PCa. Overexpression of HOXA11-AS increased PCa cell proliferat...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jiankang Zhang Sailian Li Mengyu Zhang Zhenting Wang Zengshu Xing Source Type: research
Exosomal circSTRBP from cancer cells facilitates gastric cancer progression via regulating miR-1294/miR-593-3p/E2F2 axis
J Cell Mol Med. 2024 Apr;28(8):e18217. doi: 10.1111/jcmm.18217.ABSTRACTCircRNAs represent a new class of non-coding RNAs which show aberrant expression in diverse cancers, such as gastric cancer (GC). circSTRBP, for instance, is suggested to be overexpressed in GC cells and tissues. However, the biological role of circSTRBP in the progression of GC and the potential mechanisms have not been investigated. circSTRBP levels within GC cells and tissues were measured by RT-qPCR. The stability of circSTRBP was assessed by actinomycin D and Ribonuclease R treatment. Cell proliferation, migration, invasion and in vitro angiogenic ...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yin Wang Rong Zou Deke Li Xiankui Gao Xingjun Lu Source Type: research
Geniposidic acid alleviates osteoarthritis progression through inhibiting inflammation and chondrocytes ferroptosis
In conclusion, GPA alleviates OA progression through inhibiting inflammation and chondrocytes ferroptosis via Nrf2 signalling pathway.PMID:38520209 | PMC:PMC10960175 | DOI:10.1111/jcmm.18228 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jiayang Sun Xianji Song Cuijie Wang Qing Ruan Source Type: research
TRIM52 knockdown inhibits proliferation, inflammatory responses and oxidative stress in IL-1 β-induced synovial fibroblasts to alleviate temporomandibular joint osteoarthritis
J Cell Mol Med. 2024 Apr;28(8):e18244. doi: 10.1111/jcmm.18244.ABSTRACTTo explore the mechanism of tripartite motif 52 (TRIM52) in the progression of temporomandibular joint osteoarthritis (TMJOA). Gene and protein expression were tested by quantitative real-time polymerase chain reaction and western blot, respectively. The levels of pro-inflammatory cytokines and oxidative stress factors were evaluated using enzyme-linked immunosorbent assay and biochemical kit, respectively. Cell counting kit-8 and 5-ethynyl-2'-deoxyuridine assays were carried out to assess cell proliferation. Immunofluorescence was used to detect the ex...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Tie Ma Chuan-Bin Wu Qing-Xia Shen Qiang Wang Qing Zhou Source Type: research
Transcriptomics analysis of LINC02202/XBP1 axis in melanoma: Implications for drug targeting and PD-1 monoclonal antibody efficacy
J Cell Mol Med. 2024 Apr;28(8):e18247. doi: 10.1111/jcmm.18247.ABSTRACTMalignant melanoma (MM) is a highly aggressive and deadly form of skin cancer, primarily caused by recurrence and metastasis. Therefore, it is crucial to investigate the regulatory mechanisms underlying melanoma recurrence and metastasis. Our study has identified a potential targeted regulatory relationship between LINC02202, miR-526b-3p and XBP1 in malignant melanoma. Through the regulation of the miR-526b-3p/XBP1 signalling pathway, LINC02202 may play a role in tumour progression and immune infiltration and inhibiting the expression of LINC02202 can i...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yuanyuan Shang Haiqian Yang Jian Cui Lipeng Wang Le Wang Yuan Wang Miao-Miao Zhao Pei-Yao Yu Hui Qiao Wen-Jun Yang Source Type: research
Hepatocyte-derived exosomes deliver the lncRNA CYTOR to hepatic stellate cells and promote liver fibrosis
In this study, we explored the molecular mechanisms by which damaged liver cells activate HSCs through extracellular vesicles. We established a coculture model of LO2 and LX2 and validated its exosomal transmission activity. Subsequently, differentially expressed long noncoding RNAs (lncRNAs) were screened through RNA sequencing and their mechanisms of action as competing endogenous RNAs (ceRNAs) further confirmed using biological methods, such as FISH and luciferase assays. Damaged liver cells induced activation of LX2 and upregulation of liver fibrosis-related markers. Exosomes extracted and identified from the supernata...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Wenqiang Xu Wenhui Mo Dengyu Han Weiqi Dai Xiaorong Xu Jingjing Li Xuanfu Xu Source Type: research
Histone modification-linked prognostic model for ovarian cancer reveals LBX2 as a novel growth promoter
This study aimed to investigate the role of histone modification-related genes in predicting the prognosis of OC patients. Transcriptome data from multiple cohorts, including bulk RNA-Seq data and single-cell scRNA-Seq data, were collected. Gene set enrichment analysis was used to identify enriched gene sets in the histone modification pathway. Differentially expressed genes (DEGs) between histone modification-high and histone modification-low groups were identified using Lasso regression. A prognostic model was constructed using five selected prognostic genes from the DEGs in the TCGA-OV cohort. The study found enrichment...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jian Xiong Hongyuan Liang Xiang Sun Kefei Gao Source Type: research
MAOB expression correlates with a favourable prognosis in prostate cancer, and its genetic variants are associated with the metastasis of the disease
J Cell Mol Med. 2024 Apr;28(8):e18229. doi: 10.1111/jcmm.18229.ABSTRACTMonoamine oxidase B (MAOB), a neurotransmitter-degrading enzyme, was reported to reveal conflicting roles in various cancers. However, the functional role of MAOB and impacts of its genetic variants on prostate cancer (PCa) is unknown. Herein, we genotyped four loci of MAOB single-nucleotide polymorphisms (SNPs), including rs1799836 (A/G), rs3027452 (G/A), rs6651806 (A/C) and rs6324 (G/A) in 702 PCa Taiwanese patients. We discovered that PCa patients carrying the MAOB rs6324 A-allele exhibited an increased risk of having a high initial prostate-specific...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Hsiang-Ching Huang Yi-Hsien Hsieh Chi-Hao Hsiao Chia-Yen Lin Shian-Shiang Wang Kuo-Hao Ho Lun-Ching Chang Huei-Mei Huang Shun-Fa Yang Ming-Hsien Chien Source Type: research
Prognostic and immunotherapeutic potential of regulatory T cell-associated signature in ovarian cancer
J Cell Mol Med. 2024 Apr;28(8):e18248. doi: 10.1111/jcmm.18248.ABSTRACTTumour-induced immunosuppressive microenvironments facilitate oncogenesis, with regulatory T cells (Tregs) serving as a crucial component. The significance of Treg-associated genes within the context of ovarian cancer (OC) remains elucidated insufficiently. Utilizing single-cell RNA sequencing (scRNA-Seq) for the identification of Treg-specific biomarkers, this investigation employed single-sample gene set enrichment analysis (ssGSEA) for the derivation of a Treg signature score. Weighted gene co-expression network analysis (WGCNA) facilitated the ident...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yinglei Liu Feng Shan Ying Sun Haili Kai Yang Cao Menghui Huang Jinhui Liu Pengpeng Zhang Yanli Zheng Source Type: research
Construction of a prognostic model with CAFs for predicting the prognosis and immunotherapeutic response of lung squamous cell carcinoma
J Cell Mol Med. 2024 Apr;28(8):e18262. doi: 10.1111/jcmm.18262.ABSTRACTLung squamous cell carcinoma (LUSC) is one of the subtypes of lung cancer (LC) that contributes to approximately 25%-30% of its prevalence. Cancer-associated fibroblasts (CAFs) are key cellular components of the TME, and the large number of CAFs in tumour tissues creates a favourable environment for tumour development. However, the function of CAFs in the LUSC is complex and uncertain. First, we processed the scRNA-seq data and classified distinct types of CAFs. We also identified prognostic CAFRGs using univariate Cox analysis and conducted survival an...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Xiang Zhang Qingqing Xiao Cong Zhang Qinghua Zhou Tao Xu Source Type: research
Intracerebroventricular BDNF infusion may reduce cerebral ischemia/reperfusion injury by promoting autophagy and suppressing apoptosis
J Cell Mol Med. 2024 Apr;28(8):e18246. doi: 10.1111/jcmm.18246.ABSTRACTHere, it was aimed to investigate the effects of intracerebroventricular (ICV) Brain Derived Neurotrophic Factor (BDNF) infusion for 7 days following cerebral ischemia (CI) on autophagy in neurons in the penumbra. Focal CI was created by the occlusion of the right middle cerebral artery. A total of 60 rats were used and divided into 4 groups as Control, Sham CI, CI and CI + BDNF. During the 7-day reperfusion period, aCSF (vehicle) was infused to Sham CI and CI groups, and BDNF infusion was administered to the CI + BDNF group via an osmotic minipump. By ...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Umit Yilmaz Kevser Tanbek Semir Gul Ahmet Koc Mehmet Gul Suleyman Sandal Source Type: research
Targeting HOXA11-AS to mitigate prostate cancer via the glycolytic metabolism: In vitro and in vivo
In this study, PCa cell lines were selected to assess the PCa regulatory role of HOXA11-AS overexpression versus silencing in vitro, and tumour xenografts were performed in nude mice to assess tumour suppression by HOXA11-AS silencing in vivo. HOXA11-AS expression was significantly correlated with clinicopathological factors, epithelial-mesenchymal transition (EMT) and glycolysis. Moreover, key genes downstream of HOXA11-AS exhibited good clinical diagnostic properties for PCa. Furthermore, we studied both in vitro and in vivo effects of HOXA11-AS expression on PCa. Overexpression of HOXA11-AS increased PCa cell proliferat...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jiankang Zhang Sailian Li Mengyu Zhang Zhenting Wang Zengshu Xing Source Type: research
Exosomal circSTRBP from cancer cells facilitates gastric cancer progression via regulating miR-1294/miR-593-3p/E2F2 axis
J Cell Mol Med. 2024 Apr;28(8):e18217. doi: 10.1111/jcmm.18217.ABSTRACTCircRNAs represent a new class of non-coding RNAs which show aberrant expression in diverse cancers, such as gastric cancer (GC). circSTRBP, for instance, is suggested to be overexpressed in GC cells and tissues. However, the biological role of circSTRBP in the progression of GC and the potential mechanisms have not been investigated. circSTRBP levels within GC cells and tissues were measured by RT-qPCR. The stability of circSTRBP was assessed by actinomycin D and Ribonuclease R treatment. Cell proliferation, migration, invasion and in vitro angiogenic ...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yin Wang Rong Zou Deke Li Xiankui Gao Xingjun Lu Source Type: research
Geniposidic acid alleviates osteoarthritis progression through inhibiting inflammation and chondrocytes ferroptosis
In conclusion, GPA alleviates OA progression through inhibiting inflammation and chondrocytes ferroptosis via Nrf2 signalling pathway.PMID:38520209 | DOI:10.1111/jcmm.18228 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jiayang Sun Xianji Song Cuijie Wang Qing Ruan Source Type: research
