SBFI26 induces triple-negative breast cancer cells ferroptosis via lipid peroxidation
J Cell Mol Med. 2024 Apr;28(7):e18212. doi: 10.1111/jcmm.18212.ABSTRACTSBFI26, an inhibitor of FABP5, has been shown to suppress the proliferation and metastasis of tumour cells. However, the underlying mechanism by which SBFI26 induces ferroptosis in breast cancer cells remains largely unknown. Three breast cancer cell lines were treated with SBFI26 and CCK-8 assessed cytotoxicity. Transcriptome was performed on the Illumina platform and verified by qPCR. Western blot evaluated protein levels. Malondialdehyde (MDA), total superoxide dismutase (T-SOD), Fe, glutathione (GSH) and oxidized glutathione (GSSG) were measured. SB...
Source: J Cell Mol Med - March 22, 2024 Category: Molecular Biology Authors: Gang He Yiyuan Zhang Yanjiao Feng Tangcong Chen Mei Liu Yue Zeng Xiaojing Yin Shaokui Qu Lifen Huang Youqiang Ke Li Liang Jun Yan Wei Liu Source Type: research

Immune patterns of cuproptosis in ischemic heart failure: A transcriptome analysis
In conclusion, this study elucidates the significant influence of cuproptosis on the immune microenvironment in ischemic heart failure (IHF), providing valuable insights for future mechanistic research exploring the association between cuproptosis and IHF.PMID:38509725 | DOI:10.1111/jcmm.18187 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Zhebin Chen Yunhui Zhu Songzan Chen Zhengwei Li Guosheng Fu Yao Wang Source Type: research

FLRT3 and TGF- β/SMAD4 signalling: Impacts on apoptosis, autophagy and ion channels in supraventricular tachycardia
J Cell Mol Med. 2024 Apr;28(7):e18237. doi: 10.1111/jcmm.18237.ABSTRACTTo explore the underlying molecular mechanisms of supraventricular tachycardia (SVT), this study aimed to analyse the complex relationship between FLRT3 and TGF-β/SMAD4 signalling pathway, which affects Na+ and K+ channels in cardiomyocytes. Bioinformatics analysis was performed on 85 SVT samples and 15 healthy controls to screen overlapping genes from the key module and differentially expressed genes (DEGs). Expression profiling of overlapping genes, coupled with Receiver Operating Characteristic (ROC) curve analyses, identified FLRT3 as a hub gene. I...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Yang Pang Ye Xu Qingxing Chen Kuan Cheng Yunlong Ling Jun Jang Junbo Ge Wenqing Zhu Source Type: research

HBI-8000 improves heart failure with preserved ejection fraction via the TGF- β1/MAPK signalling pathway
In conclusion, our data suggest that HBI-8000 inhibits fibrosis by modulating the TGF-β1/MAPK pathway thereby improving HFpEF. Therefore, HBI-8000 may become a new hope for the treatment of HFpEF patients.PMID:38509729 | DOI:10.1111/jcmm.18238 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Jing Tian Wenjing Li Lu Zeng Yang Li Jiamin Du Ying Li Bin Li Guohai Su Source Type: research

Induced pluripotent stem cell-based assays recapture multiple properties of human astrocytes
J Cell Mol Med. 2024 Apr;28(7):e18214. doi: 10.1111/jcmm.18214.ABSTRACTThe majority of the population of glial cells in the central nervous system consists of astrocytes, and impairment of astrocytes causes various disorders. It is useful to assess the multiple astrocytic properties in order to understand their complex roles in the pathophysiology. Although we can differentiate human astrocytes from induced pluripotent stem cells (iPSCs), it remains unknown how we can analyse and reveal the multiple properties of astrocytes in complexed human disease conditions. For this purpose, we tested astrocytic differentiation protoc...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Hideki Nonaka Takayuki Kondo Mika Suga Ryu Yamanaka Yukako Sagara Kayoko Tsukita Naoko Mitsutomi Kengo Homma Ryuta Saito Fumihiko Miyoshi Hiromitsu Ohzeki Masahiro Okuyama Haruhisa Inoue Source Type: research

Retraction: Increased Six1 expression in macrophages promotes hepatocellular carcinoma growth and invasion by regulating MMP-9
J Cell Mol Med. 2024 Apr;28(7):e18249. doi: 10.1111/jcmm.18249.ABSTRACTYongyu Zhang, Shiji Wang, Zhongmin Liu, Lewei Yang, Jian Liu, Ming Xiu. Increased Six1 expression in macrophages promotes hepatocellular carcinoma growth and invasion by regulating MMP-9. J Cell Mol Med. 2019; 23: 4523-4533. https://doi.org/10.1111/jcmm.14342. The above article, published online on 02 May 2019 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-in-Chief, Stefan Constantinescu, The Foundation for Cellular and Molecular Medicine and John Wiley and Sons Ltd. The authors ...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Source Type: research

Understanding formation processes of calcareous nephrolithiasis in renal interstitium and tubule lumen
J Cell Mol Med. 2024 Apr;28(7):e18235. doi: 10.1111/jcmm.18235.ABSTRACTKidney stone, one of the oldest known diseases, has plagued humans for centuries, consistently imposing a heavy burden on patients and healthcare systems worldwide due to their high incidence and recurrence rates. Advancements in endoscopy, imaging, genetics, molecular biology and bioinformatics have led to a deeper and more comprehensive understanding of the mechanism behind nephrolithiasis. Kidney stone formation is a complex, multi-step and long-term process involving the transformation of stone-forming salts from free ions into asymptomatic or sympt...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Caitao Dong Jiawei Zhou Xiaozhe Su Ziqi He Qianlin Song Chao Song Hu Ke Chuan Wang Wenbiao Liao Sixing Yang Source Type: research

Catalpol promotes articular cartilage repair by enhancing the recruitment of endogenous mesenchymal stem cells
In this study, full-thickness articular cartilage defect was induced in a mouse model via surgery. The animals were intraperitoneally injected with CA for 4 or 8 weeks. According to the results of macroscopic observation, micro-computed tomography CT (μCT), histological and immunohistochemistry staining, CA treatment could promote mouse cartilage repair, resulting in cartilage regeneration, bone structure improvement and matrix anabolism. Specifically, an increase in the expression of CD90, the marker of mesenchymal stem cells (MSCs), in the cartilage was observed. In addition, we evaluated the migratory and chondrogenic ...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Congzi Wu Zhenyu Shi Qinwen Ge HuiHui Xu Zhen Wu Peijian Tong Hongting Jin Source Type: research

NGCN: Drug-target interaction prediction by integrating information and feature learning from heterogeneous network
This article proposes a novel method, NGCN, to predict drug-target interactions from an integrated heterogeneous network, from which to extract relevant biological properties and association information while maintaining the topology information. It focuses on learning the topology representation of drugs and targets to improve the performance of DTI prediction. Unlike traditional methods, it focuses on learning the low-dimensional topology representation of drugs and targets via graph-based convolutional neural network. NGCN achieves substantial performance improvements over other state-of-the-art methods, such as a nearl...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Junyue Cao Qingfeng Chen Junlai Qiu Yiming Wang Wei Lan Xiaojing Du Kai Tan Source Type: research

Deficiency of heme oxygenase 1a causes detrimental effects on cardiac function
J Cell Mol Med. 2024 Apr;28(7):e18243. doi: 10.1111/jcmm.18243.ABSTRACTHumans lacking heme oxygenase 1 (HMOX1) display growth retardation, haemolytic anaemia, and vulnerability to stress; however, cardiac function remains unclear. We aimed to explore the cardiac function of zebrafish lacking hmox1a at baseline and in response to stress. We generated zebrafish hmox1a mutants using CRISPR/Cas9 genome editing technology. Deletion of hmox1a increases cardiac output and further induces hypertrophy in adults. Adults lacking hmox1a develop myocardial interstitial fibrosis, restrain cardiomyocyte proliferation and downregulate ren...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Hong Wang Juuso Siren Sanni Perttunen Katariina Immonen Yu-Chia Chen Suneeta Narumanchi Riikka Kosonen Jere Paavola Mika Laine Ilkka Tikkanen P äivi Lakkisto Source Type: research

Electroacupuncture suppresses neuronal ferroptosis to relieve chronic neuropathic pain
In this study, to investigate the detailed mechanism underlying EA intervention on NP, male Sprague-Dawley rats with chronic constriction injury (CCI)-induced NP model received EA treatment at acupoints ST36 and GV20 for 14 days. Results demonstrated that EA effectively attenuated CCI-induced pain hypersensitivity and mitigated neuron damage and loss in the spinal cord of NP rats. Moreover, EA reversed the oxidative stress-mediated spinal ferroptosis phenotype by upregulating reduced expression of xCT, glutathione peroxidase 4 (GPX4), ferritin heavy chain (FTH1) and superoxide dismutase (SOD) levels, and downregulating inc...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Chunchun Xue Wenyun Kui Aiping Huang Yanan Li Lingxing Li Zhen Gu Lei Xie Shuyi Kong Jun Yu Hongfeng Ruan Kaiqiang Wang Source Type: research

Necroptosis pathway emerged as potential diagnosis markers in spinal cord injury
J Cell Mol Med. 2024 Apr;28(7):e18219. doi: 10.1111/jcmm.18219.ABSTRACTThe present research focused on identifying necroptosis-related differentially expressed genes (NRDEGs) in spinal cord injury (SCI) to highlight potential therapeutic and prognostic target genes in clinical SCI. Three SCI-related datasets were downloaded, including GSE151371, GSE5296 and GSE47681. MSigDB and KEGG datasets were searched for necroptosis-related genes (NRGs). Differentially expressed genes (DEGs) and NRGs were intersected to obtain NRDEGs. The MCC algorithm was employed to select the first 10 genes as hub genes. A protein-protein interacti...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Jingcheng Liu Jiang Cao Xiao Yu Jie Chang Tao Sui Xiaojian Cao Source Type: research

Randomized clinical trial of continuous transversus thoracis muscle plane block for patients undergoing open heart valve replacement surgery
J Cell Mol Med. 2024 Apr;28(7):e18184. doi: 10.1111/jcmm.18184.ABSTRACTThe optimal analgesia regimen after open cardiac surgery is unclear. The aim of this study was to investigate the beneficial effects of continuous transversus thoracis muscle plane (TTMP) blocks initiated before surgery on open cardiac surgery outcomes. A group of 110 patients were randomly allocated to either receive bilateral continuous TTMP blocks (TTP group) or no nerve block (SAL group). The primary endpoint was post-operative pain at 4, 8, 16, 24, 48 and 72 h after extubation at rest and exercise. The secondary outcome measures included analgesia ...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Yanping Zhan Lei Li Shibiao Chen Yongbao Peng Yang Zhang Source Type: research

ICA/SDF-1 α/PBMSCs loaded onto alginate and gelatin cross-linked scaffolds promote damaged cartilage repair
J Cell Mol Med. 2024 Apr;28(7):e18236. doi: 10.1111/jcmm.18236.ABSTRACTA three-dimensional alginate-coated scaffold (GAIS) was constructed in the present study to showcase the multidifferentiation potential of peripheral blood mesenchymal stem cells (PBMSCs) and to investigate the role and mechanism by which Icariin (ICA)/stromal cell-derived factor (SDF-1α)/PBMSCs promote damaged articular repair. In addition, the ability of ICA, in combination with SDF-1α, to promote the migration and proliferation of stem cells was validated through the utilization of CCK-8 and migration experiments. The combination of ICA and SDF-1α...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Pengzhen Wang Pingping Zhu Wenhui Yin Jian Wu Shaoheng Zhang Source Type: research

Neuroendocrine and cellular mechanisms in stress resilience: From hormonal influence in the CNS to mitochondrial dysfunction and oxidative stress
J Cell Mol Med. 2024 Apr;28(7):e18220. doi: 10.1111/jcmm.18220.ABSTRACTRecent advancements in neuroendocrinology challenge the long-held belief that hormonal effects are confined to perivascular tissues and do not extend to the central nervous system (CNS). This paradigm shift, propelled by groundbreaking research, reveals that synthetic hormones, notably in anti-inflammatory medications, significantly influence steroid psychosis, behavioural, and cognitive impairments, as well as neuropeptide functions. A seminal development in this field occurred in 1968 with McEven's proposal that rodent brains are responsive to glucoco...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Arghya Bhattacharya Manas Chakraborty Ananya Chanda Taha Alqahtani Ajoy Kumer Bikram Dhara Moitreyee Chattopadhyay Source Type: research