PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research
PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research
CircPVT1 promotes migration and invasion by regulating miR-490-5p/HAVCR2 axis in osteosarcoma cells
This study aims to explore the molecular mechanism underlying the involvement of circPVT1 in osteosarcoma cells. We quantified circPVT1 expression using qRT-PCR in both control and osteosarcoma cell lines. To investigate the roles of circPVT1, miR-490-5p and HAVCR2 in vitro, we separately conducted overexpression and inhibition experiments for circPVT1, miR-490-5p and HAVCR2 in HOS and U2OS cells. Cell migration was assessed through wound healing and transwell migration assays, and invasion was measured via the Matrigel invasion assay. To elucidate the regulatory mechanism of circPVT1 in osteosarcoma, a comprehensive appro...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Chunbin Zhou Lois Balmer Manshu Song Kezhou Wu Wei Wang Hu Wang Source Type: research
Development, synthesis and validation of improved c-Myc/Max inhibitors
This study underscores the potential for in vitro investigations of various tumour types using novel c-Myc inhibitors to yield ground-breaking and efficacious anticancer compounds.PMID:38568057 | PMC:PMC10989597 | DOI:10.1111/jcmm.18272 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: S ümbül Yıldırım Fatih Kocaba ş Arif Mermer Source Type: research
A novel long non-coding RNA LINC00524 facilitates invasion and metastasis through interaction with TDP43 in breast cancer
In this study, we examined the influence of the lncRNA LINC00524 on BC invasion and metastasis. Through meticulous analyses of TCGA and GEO data sets, we observed a conspicuous elevation of LINC00524 expression in BC tissues. This increased expression correlated strongly with a poorer prognosis for BC patients. A detailed Gene Ontology analysis suggested that LINC00524 likely exerts its effects through RNA-binding proteins (RBPs) mechanisms. Experimentally, LINC00524 was demonstrated to amplify BC cell migration, invasion and proliferation in vitro. Additionally, in vivo tests showed its potent role in promoting BC cell gr...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Xianglin Sun Wenfeng Li Gang Li Huan Yang Zhenglin Jiang Lihua Shen Yuexiang Shen Yifei Liu Guohua Wang Source Type: research
RhoA/rho kinase pathway activation in age-associated endothelial cell dysfunction and thrombosis
J Cell Mol Med. 2024 Apr;28(8):e18153. doi: 10.1111/jcmm.18153.ABSTRACTThe small GTPase RhoA and the downstream Rho kinase (ROCK) regulate several cell functions and pathological processes in the vascular system that contribute to the age-dependent risk of cardiovascular disease, including endothelial dysfunction, excessive permeability, inflammation, impaired angiogenesis, abnormal vasoconstriction, decreased nitric oxide production and apoptosis. Frailty is a loss of physiological reserve and adaptive capacity with advanced age and is accompanied by a pro-inflammatory and pro-oxidative state that promotes vascular dysfun...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Iv án Palomo Sergio Wehinger Vicente Andr és Francisco J Garc ía-García Eduardo Fuentes Source Type: research
Peripheral nerve-derived Sema3A promotes osteogenic differentiation of mesenchymal stem cells through the Wnt/ β-catenin/Nrp1 positive feedback loop
J Cell Mol Med. 2024 Apr;28(8):e18201. doi: 10.1111/jcmm.18201.ABSTRACTSensory nerves play a crucial role in maintaining bone homeostasis by releasing Semaphorin 3A (Sema3A). However, the specific mechanism of Sema3A in regulation of bone marrow mesenchymal stem cells (BMMSCs) during bone remodelling remains unclear. The tibial denervation model was used and the denervated tibia exhibited significantly lower mass as compared to sham operated bones. In vitro, BMMSCs cocultured with dorsal root ganglion cells (DRGs) or stimulated by Sema3A could promote osteogenic differentiation through the Wnt/β-catenin/Nrp1 positive feed...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Jingcun Shi Bingqing Zhang Ziqian Wu Yuhan Zhang Anand Gupta Xudong Wang Jieyu Wang Lisha Pan Meng Xiao Shijian Zhang Lei Wang Source Type: research
The association between serum copper concentration and prevalence of diabetes among US adults with hypertension (NHANES 2011-2016)
This study finds that serum copper concentration are significantly associated with risk of diabetes in hypertensive patients, which suggests copper as an important risk factor of diabetes development.PMID:38568081 | PMC:PMC10989603 | DOI:10.1111/jcmm.18270 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Kaiming Wu Lixia Chen Yanyan Kong Jian-Feng Zhuo Qiu Sun Jianfei Chang Source Type: research
Integrative analysis unveils ECM signatures and pathways driving hepatocellular carcinoma progression: A multi-omics approach and prognostic model development
J Cell Mol Med. 2024 Apr;28(8):e18230. doi: 10.1111/jcmm.18230.ABSTRACTLiver hepatocellular carcinoma (LIHC) is a highly lethal form of cancer that is among the deadliest cancer types globally. In terms of cancer-related mortality rates, liver cancer ranks among the top three, underscoring the severity of this disease. Insufficient analysis has been conducted to fully understand the potential value of the extracellular matrix (ECM) in immune infiltration and the prognostic stratification of LIHC, despite its recognised importance in the development of this disease. The scRNA-seq data of GSE149614 was used to conduct single...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Zhen Liu Pengfei Zhao Source Type: research
Bergamot essential oil improves CUMS-induced depression-like behaviour in rats by protecting the plasticity of hippocampal neurons
This study was to investigate the effects and the underlying mechanism of BEO in mitigating depression. GC-MS were used to identify its constituents. Antidepressive properties of BEO were evaluated by sucrose preference test (SPT), force swimming test (FST) and open field test (OFT). Nissl staining was used to determine the number of Nissl bodies in hippocampus (HIPP) of rats. Changes in HIPP dendritic length and dendritic spine density were detected by Golgi-Cox staining. Immunohistochemistry and Western blot were used to detect the postsynaptic density protein-95 (PSD-95) and synaptophysin (SYP) in the HIPP of rats. The ...
Source: J Cell Mol Med - March 30, 2024 Category: Molecular Biology Authors: Jun Chang Huimin Yang Xiaoqian Shan Lan Zhao Yujiao Li Zhao Zhang Joseph Kofi Abankwah Mingxing Zhang Yuhong Bian Yi Guo Source Type: research
Bergamot essential oil improves CUMS-induced depression-like behaviour in rats by protecting the plasticity of hippocampal neurons
This study was to investigate the effects and the underlying mechanism of BEO in mitigating depression. GC-MS were used to identify its constituents. Antidepressive properties of BEO were evaluated by sucrose preference test (SPT), force swimming test (FST) and open field test (OFT). Nissl staining was used to determine the number of Nissl bodies in hippocampus (HIPP) of rats. Changes in HIPP dendritic length and dendritic spine density were detected by Golgi-Cox staining. Immunohistochemistry and Western blot were used to detect the postsynaptic density protein-95 (PSD-95) and synaptophysin (SYP) in the HIPP of rats. The ...
Source: J Cell Mol Med - March 30, 2024 Category: Molecular Biology Authors: Jun Chang Huimin Yang Xiaoqian Shan Lan Zhao Yujiao Li Zhao Zhang Joseph Kofi Abankwah Mingxing Zhang Yuhong Bian Yi Guo Source Type: research
Bergamot essential oil improves CUMS-induced depression-like behaviour in rats by protecting the plasticity of hippocampal neurons
This study was to investigate the effects and the underlying mechanism of BEO in mitigating depression. GC-MS were used to identify its constituents. Antidepressive properties of BEO were evaluated by sucrose preference test (SPT), force swimming test (FST) and open field test (OFT). Nissl staining was used to determine the number of Nissl bodies in hippocampus (HIPP) of rats. Changes in HIPP dendritic length and dendritic spine density were detected by Golgi-Cox staining. Immunohistochemistry and Western blot were used to detect the postsynaptic density protein-95 (PSD-95) and synaptophysin (SYP) in the HIPP of rats. The ...
Source: J Cell Mol Med - March 30, 2024 Category: Molecular Biology Authors: Jun Chang Huimin Yang Xiaoqian Shan Lan Zhao Yujiao Li Zhao Zhang Joseph Kofi Abankwah Mingxing Zhang Yuhong Bian Yi Guo Source Type: research
Bergamot essential oil improves CUMS-induced depression-like behaviour in rats by protecting the plasticity of hippocampal neurons
This study was to investigate the effects and the underlying mechanism of BEO in mitigating depression. GC-MS were used to identify its constituents. Antidepressive properties of BEO were evaluated by sucrose preference test (SPT), force swimming test (FST) and open field test (OFT). Nissl staining was used to determine the number of Nissl bodies in hippocampus (HIPP) of rats. Changes in HIPP dendritic length and dendritic spine density were detected by Golgi-Cox staining. Immunohistochemistry and Western blot were used to detect the postsynaptic density protein-95 (PSD-95) and synaptophysin (SYP) in the HIPP of rats. The ...
Source: J Cell Mol Med - March 30, 2024 Category: Molecular Biology Authors: Jun Chang Huimin Yang Xiaoqian Shan Lan Zhao Yujiao Li Zhao Zhang Joseph Kofi Abankwah Mingxing Zhang Yuhong Bian Yi Guo Source Type: research
Role of the gut microbiota and innate immunity in polycystic ovary syndrome: Current updates and future prospects
This study reviewed recent research advances about the role of gut microbiota in PCOS. In order to provide basis for prevention and treatment of PCOS based on gut microbiota.PMID:38546608 | PMC:PMC10977384 | DOI:10.1111/jcmm.18258 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 28, 2024 Category: Molecular Biology Authors: Min Zhou Jing Yu Xuewei Li Zheng Ruan Shaohui Yu Source Type: research
KLF transcription factors in bone diseases
J Cell Mol Med. 2024 Apr;28(8):e18278. doi: 10.1111/jcmm.18278.ABSTRACTKrüppel-like factors (KLFs) are crucial in the development of bone disease. They are a family of zinc finger transcription factors that are unusual in containing three highly conserved zinc finger structural domains interacting with DNA. It has been discovered that it engages in various cell functions, including proliferation, apoptosis, autophagy, stemness, invasion and migration, and is crucial for the development of human tissues. In recent years, the role of KLFs in bone physiology and pathology has received adequate attention. In addition to regul...
Source: J Cell Mol Med - March 28, 2024 Category: Molecular Biology Authors: Haixia Wang Juanjuan Han Gorbachev Dmitrii Ke Ning Xin-An Zhang Source Type: research
