TRIM52 knockdown inhibits proliferation, inflammatory responses and oxidative stress in IL-1 β-induced synovial fibroblasts to alleviate temporomandibular joint osteoarthritis
J Cell Mol Med. 2024 Apr;28(8):e18244. doi: 10.1111/jcmm.18244.ABSTRACTTo explore the mechanism of tripartite motif 52 (TRIM52) in the progression of temporomandibular joint osteoarthritis (TMJOA). Gene and protein expression were tested by quantitative real-time polymerase chain reaction and western blot, respectively. The levels of pro-inflammatory cytokines and oxidative stress factors were evaluated using enzyme-linked immunosorbent assay and biochemical kit, respectively. Cell counting kit-8 and 5-ethynyl-2'-deoxyuridine assays were carried out to assess cell proliferation. Immunofluorescence was used to detect the ex...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Tie Ma Chuan-Bin Wu Qing-Xia Shen Qiang Wang Qing Zhou Source Type: research
Transcriptomics analysis of LINC02202/XBP1 axis in melanoma: Implications for drug targeting and PD-1 monoclonal antibody efficacy
J Cell Mol Med. 2024 Apr;28(8):e18247. doi: 10.1111/jcmm.18247.ABSTRACTMalignant melanoma (MM) is a highly aggressive and deadly form of skin cancer, primarily caused by recurrence and metastasis. Therefore, it is crucial to investigate the regulatory mechanisms underlying melanoma recurrence and metastasis. Our study has identified a potential targeted regulatory relationship between LINC02202, miR-526b-3p and XBP1 in malignant melanoma. Through the regulation of the miR-526b-3p/XBP1 signalling pathway, LINC02202 may play a role in tumour progression and immune infiltration and inhibiting the expression of LINC02202 can i...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yuanyuan Shang Haiqian Yang Jian Cui Lipeng Wang Le Wang Yuan Wang Miao-Miao Zhao Pei-Yao Yu Hui Qiao Wen-Jun Yang Source Type: research
Hepatocyte-derived exosomes deliver the lncRNA CYTOR to hepatic stellate cells and promote liver fibrosis
In this study, we explored the molecular mechanisms by which damaged liver cells activate HSCs through extracellular vesicles. We established a coculture model of LO2 and LX2 and validated its exosomal transmission activity. Subsequently, differentially expressed long noncoding RNAs (lncRNAs) were screened through RNA sequencing and their mechanisms of action as competing endogenous RNAs (ceRNAs) further confirmed using biological methods, such as FISH and luciferase assays. Damaged liver cells induced activation of LX2 and upregulation of liver fibrosis-related markers. Exosomes extracted and identified from the supernata...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Wenqiang Xu Wenhui Mo Dengyu Han Weiqi Dai Xiaorong Xu Jingjing Li Xuanfu Xu Source Type: research
Histone modification-linked prognostic model for ovarian cancer reveals LBX2 as a novel growth promoter
This study aimed to investigate the role of histone modification-related genes in predicting the prognosis of OC patients. Transcriptome data from multiple cohorts, including bulk RNA-Seq data and single-cell scRNA-Seq data, were collected. Gene set enrichment analysis was used to identify enriched gene sets in the histone modification pathway. Differentially expressed genes (DEGs) between histone modification-high and histone modification-low groups were identified using Lasso regression. A prognostic model was constructed using five selected prognostic genes from the DEGs in the TCGA-OV cohort. The study found enrichment...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Jian Xiong Hongyuan Liang Xiang Sun Kefei Gao Source Type: research
MAOB expression correlates with a favourable prognosis in prostate cancer, and its genetic variants are associated with the metastasis of the disease
J Cell Mol Med. 2024 Apr;28(8):e18229. doi: 10.1111/jcmm.18229.ABSTRACTMonoamine oxidase B (MAOB), a neurotransmitter-degrading enzyme, was reported to reveal conflicting roles in various cancers. However, the functional role of MAOB and impacts of its genetic variants on prostate cancer (PCa) is unknown. Herein, we genotyped four loci of MAOB single-nucleotide polymorphisms (SNPs), including rs1799836 (A/G), rs3027452 (G/A), rs6651806 (A/C) and rs6324 (G/A) in 702 PCa Taiwanese patients. We discovered that PCa patients carrying the MAOB rs6324 A-allele exhibited an increased risk of having a high initial prostate-specific...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Hsiang-Ching Huang Yi-Hsien Hsieh Chi-Hao Hsiao Chia-Yen Lin Shian-Shiang Wang Kuo-Hao Ho Lun-Ching Chang Huei-Mei Huang Shun-Fa Yang Ming-Hsien Chien Source Type: research
Prognostic and immunotherapeutic potential of regulatory T cell-associated signature in ovarian cancer
J Cell Mol Med. 2024 Apr;28(8):e18248. doi: 10.1111/jcmm.18248.ABSTRACTTumour-induced immunosuppressive microenvironments facilitate oncogenesis, with regulatory T cells (Tregs) serving as a crucial component. The significance of Treg-associated genes within the context of ovarian cancer (OC) remains elucidated insufficiently. Utilizing single-cell RNA sequencing (scRNA-Seq) for the identification of Treg-specific biomarkers, this investigation employed single-sample gene set enrichment analysis (ssGSEA) for the derivation of a Treg signature score. Weighted gene co-expression network analysis (WGCNA) facilitated the ident...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Yinglei Liu Feng Shan Ying Sun Haili Kai Yang Cao Menghui Huang Jinhui Liu Pengpeng Zhang Yanli Zheng Source Type: research
Construction of a prognostic model with CAFs for predicting the prognosis and immunotherapeutic response of lung squamous cell carcinoma
J Cell Mol Med. 2024 Apr;28(8):e18262. doi: 10.1111/jcmm.18262.ABSTRACTLung squamous cell carcinoma (LUSC) is one of the subtypes of lung cancer (LC) that contributes to approximately 25%-30% of its prevalence. Cancer-associated fibroblasts (CAFs) are key cellular components of the TME, and the large number of CAFs in tumour tissues creates a favourable environment for tumour development. However, the function of CAFs in the LUSC is complex and uncertain. First, we processed the scRNA-seq data and classified distinct types of CAFs. We also identified prognostic CAFRGs using univariate Cox analysis and conducted survival an...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Xiang Zhang Qingqing Xiao Cong Zhang Qinghua Zhou Tao Xu Source Type: research
Intracerebroventricular BDNF infusion may reduce cerebral ischemia/reperfusion injury by promoting autophagy and suppressing apoptosis
J Cell Mol Med. 2024 Apr;28(8):e18246. doi: 10.1111/jcmm.18246.ABSTRACTHere, it was aimed to investigate the effects of intracerebroventricular (ICV) Brain Derived Neurotrophic Factor (BDNF) infusion for 7 days following cerebral ischemia (CI) on autophagy in neurons in the penumbra. Focal CI was created by the occlusion of the right middle cerebral artery. A total of 60 rats were used and divided into 4 groups as Control, Sham CI, CI and CI + BDNF. During the 7-day reperfusion period, aCSF (vehicle) was infused to Sham CI and CI groups, and BDNF infusion was administered to the CI + BDNF group via an osmotic minipump. By ...
Source: J Cell Mol Med - March 23, 2024 Category: Molecular Biology Authors: Umit Yilmaz Kevser Tanbek Semir Gul Ahmet Koc Mehmet Gul Suleyman Sandal Source Type: research
SBFI26 induces triple-negative breast cancer cells ferroptosis via lipid peroxidation
J Cell Mol Med. 2024 Apr;28(7):e18212. doi: 10.1111/jcmm.18212.ABSTRACTSBFI26, an inhibitor of FABP5, has been shown to suppress the proliferation and metastasis of tumour cells. However, the underlying mechanism by which SBFI26 induces ferroptosis in breast cancer cells remains largely unknown. Three breast cancer cell lines were treated with SBFI26 and CCK-8 assessed cytotoxicity. Transcriptome was performed on the Illumina platform and verified by qPCR. Western blot evaluated protein levels. Malondialdehyde (MDA), total superoxide dismutase (T-SOD), Fe, glutathione (GSH) and oxidized glutathione (GSSG) were measured. SB...
Source: J Cell Mol Med - March 22, 2024 Category: Molecular Biology Authors: Gang He Yiyuan Zhang Yanjiao Feng Tangcong Chen Mei Liu Yue Zeng Xiaojing Yin Shaokui Qu Lifen Huang Youqiang Ke Li Liang Jun Yan Wei Liu Source Type: research
SBFI26 induces triple-negative breast cancer cells ferroptosis via lipid peroxidation
J Cell Mol Med. 2024 Apr;28(7):e18212. doi: 10.1111/jcmm.18212.ABSTRACTSBFI26, an inhibitor of FABP5, has been shown to suppress the proliferation and metastasis of tumour cells. However, the underlying mechanism by which SBFI26 induces ferroptosis in breast cancer cells remains largely unknown. Three breast cancer cell lines were treated with SBFI26 and CCK-8 assessed cytotoxicity. Transcriptome was performed on the Illumina platform and verified by qPCR. Western blot evaluated protein levels. Malondialdehyde (MDA), total superoxide dismutase (T-SOD), Fe, glutathione (GSH) and oxidized glutathione (GSSG) were measured. SB...
Source: J Cell Mol Med - March 22, 2024 Category: Molecular Biology Authors: Gang He Yiyuan Zhang Yanjiao Feng Tangcong Chen Mei Liu Yue Zeng Xiaojing Yin Shaokui Qu Lifen Huang Youqiang Ke Li Liang Jun Yan Wei Liu Source Type: research
Immune patterns of cuproptosis in ischemic heart failure: A transcriptome analysis
In conclusion, this study elucidates the significant influence of cuproptosis on the immune microenvironment in ischemic heart failure (IHF), providing valuable insights for future mechanistic research exploring the association between cuproptosis and IHF.PMID:38509725 | DOI:10.1111/jcmm.18187 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Zhebin Chen Yunhui Zhu Songzan Chen Zhengwei Li Guosheng Fu Yao Wang Source Type: research
FLRT3 and TGF- β/SMAD4 signalling: Impacts on apoptosis, autophagy and ion channels in supraventricular tachycardia
J Cell Mol Med. 2024 Apr;28(7):e18237. doi: 10.1111/jcmm.18237.ABSTRACTTo explore the underlying molecular mechanisms of supraventricular tachycardia (SVT), this study aimed to analyse the complex relationship between FLRT3 and TGF-β/SMAD4 signalling pathway, which affects Na+ and K+ channels in cardiomyocytes. Bioinformatics analysis was performed on 85 SVT samples and 15 healthy controls to screen overlapping genes from the key module and differentially expressed genes (DEGs). Expression profiling of overlapping genes, coupled with Receiver Operating Characteristic (ROC) curve analyses, identified FLRT3 as a hub gene. I...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Yang Pang Ye Xu Qingxing Chen Kuan Cheng Yunlong Ling Jun Jang Junbo Ge Wenqing Zhu Source Type: research
HBI-8000 improves heart failure with preserved ejection fraction via the TGF- β1/MAPK signalling pathway
In conclusion, our data suggest that HBI-8000 inhibits fibrosis by modulating the TGF-β1/MAPK pathway thereby improving HFpEF. Therefore, HBI-8000 may become a new hope for the treatment of HFpEF patients.PMID:38509729 | DOI:10.1111/jcmm.18238 (Source: J Cell Mol Med)
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Jing Tian Wenjing Li Lu Zeng Yang Li Jiamin Du Ying Li Bin Li Guohai Su Source Type: research
Induced pluripotent stem cell-based assays recapture multiple properties of human astrocytes
J Cell Mol Med. 2024 Apr;28(7):e18214. doi: 10.1111/jcmm.18214.ABSTRACTThe majority of the population of glial cells in the central nervous system consists of astrocytes, and impairment of astrocytes causes various disorders. It is useful to assess the multiple astrocytic properties in order to understand their complex roles in the pathophysiology. Although we can differentiate human astrocytes from induced pluripotent stem cells (iPSCs), it remains unknown how we can analyse and reveal the multiple properties of astrocytes in complexed human disease conditions. For this purpose, we tested astrocytic differentiation protoc...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Authors: Hideki Nonaka Takayuki Kondo Mika Suga Ryu Yamanaka Yukako Sagara Kayoko Tsukita Naoko Mitsutomi Kengo Homma Ryuta Saito Fumihiko Miyoshi Hiromitsu Ohzeki Masahiro Okuyama Haruhisa Inoue Source Type: research
Retraction: Increased Six1 expression in macrophages promotes hepatocellular carcinoma growth and invasion by regulating MMP-9
J Cell Mol Med. 2024 Apr;28(7):e18249. doi: 10.1111/jcmm.18249.ABSTRACTYongyu Zhang, Shiji Wang, Zhongmin Liu, Lewei Yang, Jian Liu, Ming Xiu. Increased Six1 expression in macrophages promotes hepatocellular carcinoma growth and invasion by regulating MMP-9. J Cell Mol Med. 2019; 23: 4523-4533. https://doi.org/10.1111/jcmm.14342. The above article, published online on 02 May 2019 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-in-Chief, Stefan Constantinescu, The Foundation for Cellular and Molecular Medicine and John Wiley and Sons Ltd. The authors ...
Source: J Cell Mol Med - March 21, 2024 Category: Molecular Biology Source Type: research
