Engeletin alleviates depressive-like behaviours by modulating microglial polarization via the LCN2/CXCL10 signalling pathway
In conclusion, engeletin can alleviate CSDS-induced depressive-like behaviours by regulating the LCN2/CXCL10 pathway and thereby altering the polarization of microglia. These data suggest that the antidepressant effects of engeletin are correlated with the polarization of microglia, highlighting a potential avenue for future design of antidepressant strategies that specifically target the microglia.PMID:38597406 | PMC:PMC11005460 | DOI:10.1111/jcmm.18285 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 10, 2024 Category: Molecular Biology Authors: Jie Zhang Zheng Song Yanchao Huo Guangqiang Li Liming Lu Chuanmei Wei Shuping Zhang Xinfu Gao Xingyue Jiang Yangyang Xu Source Type: research

A single-chain antibody construct with specificity of a natural IgM antibody reduces hepatic ischemia reperfusion injury in mice
J Cell Mol Med. 2024 Apr;28(8):e18291. doi: 10.1111/jcmm.18291.ABSTRACTNatural immunoglobulin M (IgM) antibodies have been shown to recognize post-ischemic neoepitopes following reperfusion of tissues and to activate complement. Specifically, IgM antibodies and complement have been shown to drive hepatic ischemia reperfusion injury (IRI). Herein, we investigate the therapeutic effect of C2 scFv (single-chain antibody construct with specificity of a natural IgM antibody) on hepatic IRI in C57BL/6 mice. Compared with PBS-treated mice, C2 scFv-treated mice displayed almost no necrotic areas, significant reduction in serum ALT...
Source: J Cell Mol Med - April 10, 2024 Category: Molecular Biology Authors: Zhi Yang Chunmei Li Yongqin Wang Wei Dong Moujie Yang Junfei Jin Source Type: research

Purine metabolism in lung adenocarcinoma: A single-cell analysis revealing prognostic and immunotherapeutic insights
J Cell Mol Med. 2024 Apr;28(8):e18284. doi: 10.1111/jcmm.18284.ABSTRACTLung adenocarcinoma (LUAD) is a prevalent subtype of lung cancer, yet the contribution of purine metabolism (PM) to its pathogenesis remains poorly elucidated. PM, a critical component of intracellular nucleotide synthesis and energy metabolism, is hypothesized to exert a significant influence on LUAD development. Herein, we employed single-cell analysis to investigate the role of PM within the tumour microenvironment (TME) of LUAD. PM scoring (PMS) across distinct cell types was determined using AUCell, UCell, singscore and AddModuleScore algorithms. S...
Source: J Cell Mol Med - April 10, 2024 Category: Molecular Biology Authors: Pengpeng Zhang Shengbin Pei Guangyao Zhou Mengzhe Zhang Lianmin Zhang Zhenfa Zhang Source Type: research

Irradiated riboflavin over nonradiated one: Potent antimigratory, antiproliferative and cytotoxic effects on glioblastoma cells
In conclusion, radiated riboflavin clearly demonstrates superior antiproliferative and apoptotic effects on C6 cells at lower doses compared to nonradiated riboflavin.PMID:38597418 | PMC:PMC11005454 | DOI:10.1111/jcmm.18288 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 10, 2024 Category: Molecular Biology Authors: Sedat Kacar Ceyhan Hacioglu Fatih Kar Source Type: research

Capsiate ameliorates secondary hyperparathyroidism by improving insulin sensitivity and inhibiting angiogenesis
This study aims to evaluate the effects and mechanisms of capsiates in the treatment of secondary hyperparathyroidism. To achieve our research objectives, we conducted a study on patients' serum and examined changes in metabolic markers using serum metabolomics. We induced secondary hyperparathyroidism in rat through dietary intervention and divided them into four groups. The first group, referred to as the Parathyroid Hormone (PTH) group, received a low-calcium and high-phosphate diet (0.2% calcium, 1.2% phosphorus). The second group served as the control group, receiving a standard phosphate and calcium diet (0.6% calciu...
Source: J Cell Mol Med - April 9, 2024 Category: Molecular Biology Authors: Peiting Li Jianda Zhou Tianyin Wang Jun Li Wei Wu Source Type: research

Capsiate ameliorates secondary hyperparathyroidism by improving insulin sensitivity and inhibiting angiogenesis
This study aims to evaluate the effects and mechanisms of capsiates in the treatment of secondary hyperparathyroidism. To achieve our research objectives, we conducted a study on patients' serum and examined changes in metabolic markers using serum metabolomics. We induced secondary hyperparathyroidism in rat through dietary intervention and divided them into four groups. The first group, referred to as the Parathyroid Hormone (PTH) group, received a low-calcium and high-phosphate diet (0.2% calcium, 1.2% phosphorus). The second group served as the control group, receiving a standard phosphate and calcium diet (0.6% calciu...
Source: J Cell Mol Med - April 9, 2024 Category: Molecular Biology Authors: Peiting Li Jianda Zhou Tianyin Wang Jun Li Wei Wu Source Type: research

GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling
J Cell Mol Med. 2024 Apr;28(8):e18290. doi: 10.1111/jcmm.18290.ABSTRACTGrowth hormone inducible transmembrane protein (GHITM), one member of Bax inhibitory protein-like family, has been rarely studied, and the clinical importance and biological functions of GHITM in kidney renal clear cell carcinoma (KIRC) still remain unknown. In the present study, we found that GHITM was downregulated in KIRC. Aberrant GHITM downregulation related to clinicopathological feature and unfavourable prognosis of KIRC patients. GHITM overexpression inhibited KIRC cell proliferation, migration and invasion in vitro and in vivo. Mechanistically,...
Source: J Cell Mol Med - April 8, 2024 Category: Molecular Biology Authors: Shiyu Huang Jiachen Liu Juncheng Hu Yanguang Hou Min Hu Banghua Zhang Hongbo Luo Shujie Fu Yujie Chen Xiuheng Liu Zhiyuan Chen Lei Wang Source Type: research

GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling
J Cell Mol Med. 2024 Apr;28(8):e18290. doi: 10.1111/jcmm.18290.ABSTRACTGrowth hormone inducible transmembrane protein (GHITM), one member of Bax inhibitory protein-like family, has been rarely studied, and the clinical importance and biological functions of GHITM in kidney renal clear cell carcinoma (KIRC) still remain unknown. In the present study, we found that GHITM was downregulated in KIRC. Aberrant GHITM downregulation related to clinicopathological feature and unfavourable prognosis of KIRC patients. GHITM overexpression inhibited KIRC cell proliferation, migration and invasion in vitro and in vivo. Mechanistically,...
Source: J Cell Mol Med - April 8, 2024 Category: Molecular Biology Authors: Shiyu Huang Jiachen Liu Juncheng Hu Yanguang Hou Min Hu Banghua Zhang Hongbo Luo Shujie Fu Yujie Chen Xiuheng Liu Zhiyuan Chen Lei Wang Source Type: research

PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research

PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research

PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research

PGC-1 α regulates the interplay between oxidative stress, senescence and autophagy in the ageing retina important in age-related macular degeneration
J Cell Mol Med. 2024 Apr;28(8):e18051. doi: 10.1111/jcmm.18051.ABSTRACTWe previously showed that mice with knockout in the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) gene encoding the PGC-1α protein, and nuclear factor erythroid 2 like 2 (NFE2L2) gene, exhibited some features of the age-related macular degeneration (AMD) phenotype. To further explore the mechanism behind the involvement of PGC-1α in AMD pathogenesis we used young (3-month) and old (12-month) mice with knockout in the PPARGC1A gene and age-matched wild-type (WT) animals. An immunohistochemical analysis showed age-depen...
Source: J Cell Mol Med - April 4, 2024 Category: Molecular Biology Authors: Iswariyaraja Sridevi Gurubaran Cezary Watala Joanna Kostanek Joanna Szczepanska Elzbieta Pawlowska Kai Kaarniranta Janusz Blasiak Source Type: research

CircPVT1 promotes migration and invasion by regulating miR-490-5p/HAVCR2 axis in osteosarcoma cells
This study aims to explore the molecular mechanism underlying the involvement of circPVT1 in osteosarcoma cells. We quantified circPVT1 expression using qRT-PCR in both control and osteosarcoma cell lines. To investigate the roles of circPVT1, miR-490-5p and HAVCR2 in vitro, we separately conducted overexpression and inhibition experiments for circPVT1, miR-490-5p and HAVCR2 in HOS and U2OS cells. Cell migration was assessed through wound healing and transwell migration assays, and invasion was measured via the Matrigel invasion assay. To elucidate the regulatory mechanism of circPVT1 in osteosarcoma, a comprehensive appro...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Chunbin Zhou Lois Balmer Manshu Song Kezhou Wu Wei Wang Hu Wang Source Type: research

Development, synthesis and validation of improved c-Myc/Max inhibitors
This study underscores the potential for in vitro investigations of various tumour types using novel c-Myc inhibitors to yield ground-breaking and efficacious anticancer compounds.PMID:38568057 | PMC:PMC10989597 | DOI:10.1111/jcmm.18272 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: S ümbül Yıldırım Fatih Kocaba ş Arif Mermer Source Type: research

A novel long non-coding RNA LINC00524 facilitates invasion and metastasis through interaction with TDP43 in breast cancer
In this study, we examined the influence of the lncRNA LINC00524 on BC invasion and metastasis. Through meticulous analyses of TCGA and GEO data sets, we observed a conspicuous elevation of LINC00524 expression in BC tissues. This increased expression correlated strongly with a poorer prognosis for BC patients. A detailed Gene Ontology analysis suggested that LINC00524 likely exerts its effects through RNA-binding proteins (RBPs) mechanisms. Experimentally, LINC00524 was demonstrated to amplify BC cell migration, invasion and proliferation in vitro. Additionally, in vivo tests showed its potent role in promoting BC cell gr...
Source: J Cell Mol Med - April 3, 2024 Category: Molecular Biology Authors: Xianglin Sun Wenfeng Li Gang Li Huan Yang Zhenglin Jiang Lihua Shen Yuexiang Shen Yifei Liu Guohua Wang Source Type: research