Still water run deep: Therapeutic TP effect of ucMSC-Ex via regulating mTOR to enhance autophagy
J Cell Mol Med. 2024 Feb;28(4):e18120. doi: 10.1111/jcmm.18120.ABSTRACTOur previous study confirmed that umbilical cord mesenchymal stem cells-exosomes (ucMSC-Ex) inhibit apoptosis of pancreatic acinar cells to exert protective effects. However, the relationship between apoptosis and autophagy in traumatic pancreatitis (TP) has rarely been reported. We dissected the transcriptomics after pancreatic trauma and ucMSC-Ex therapy by high-throughput sequencing. Additionally, we used rapamycin and MHY1485 to regulate mTOR. HE, inflammatory factors and pancreatic enzymatic assays were used to comprehensively determine the local v...
Source: J Cell Mol Med - February 15, 2024 Category: Molecular Biology Authors: Zhirong Zhao Li Han Mei Xin Lichen Zhou Kexin Jiang Qian Huang Ruiwu Dai Source Type: research
Atractylodes macrocephala III suppresses EMT in cervical cancer by regulating IGF2BP3 through ETV5
In this study, we found that ATL III promotes apoptosis and regulates epithelial-mesenchymal transition (EMT) in cervical cancer cells (HeLa and SiHa cells) and that IGF2BP3 is a common target gene of ATL III in HeLa and SiHa cells. The expression level of IGF2BP3 in cervical cancer cells was proportional to their migration and invasion abilities. This was verified by transfection of cells with a small interfering RNA and an IGF2BP3 overexpression plasmid. After ATL III treatment, the migration and invasion abilities of cervical cancer cells were obviously reduced, but these effects were attenuated after overexpression of ...
Source: J Cell Mol Med - February 15, 2024 Category: Molecular Biology Authors: Meixia Wang Jingwen Meng Hongyun Wang Huijuan Hu Ying Hong Source Type: research
Exosome inhibition improves response to first-line therapy in small cell lung cancer
J Cell Mol Med. 2024 Feb;28(4):e18138. doi: 10.1111/jcmm.18138.ABSTRACTExosomes are recognized as important mediators of cell-to-cell communication, facilitating carcinogenesis. Although there have been significant advancements in exosome research in recent decades, no drugs that target the inhibition of sEV secretion have been approved for human use. For this study, we employed GW4869 and Nexinhib20 as inhibitors of exosome synthesis and trafficking combined. First, we found that Nexinhib20 and GW4869 effectively inhibited RAB27A and neutral sphingomyelinase 2 (nSMase2) nsMase2. Interestingly, the inhibition of nsMase2 an...
Source: J Cell Mol Med - February 14, 2024 Category: Molecular Biology Authors: Nesrin Irep Kubilay Inci Pervin Elvan Tokgun Onur Tokgun Source Type: research
Targeting type H vessels in bone-related diseases
J Cell Mol Med. 2024 Feb;28(4):e18123. doi: 10.1111/jcmm.18123.ABSTRACTBlood vessels are essential for bone development and metabolism. Type H vessels in bone, named after their high expression of CD31 and Endomucin (Emcn), have recently been reported to locate mainly in the metaphysis, exhibit different molecular properties and couple osteogenesis and angiogenesis. A strong correlation between type H vessels and bone metabolism is now well-recognized. The crosstalk between type H vessels and osteoprogenitor cells is also involved in bone metabolism-related diseases such as osteoporosis, osteoarthritis, fracture healing an...
Source: J Cell Mol Med - February 14, 2024 Category: Molecular Biology Authors: Juan Xu Shuang-Jian He Ting-Ting Xia Yu Shan Liang Wang Source Type: research
α-Solanine attenuates chondrocyte pyroptosis to improve osteoarthritis via suppressing NF-κB pathway
J Cell Mol Med. 2024 Feb;28(4):e18132. doi: 10.1111/jcmm.18132.ABSTRACTα-Solanine has been shown to exhibit anti-inflammatory and anti-tumour properties; however, its efficacy in treating osteoarthritis (OA) remains ambiguous. The study aimed to evaluate the therapeutic effects of α-solanine on OA development in a mouse OA model. The OA mice were subjected to varying concentrations of α-solanine, and various assessments were implemented to assess OA progression. We found that α-solanine significantly reduced osteophyte formation, subchondral sclerosis and OARSI score. And it decreased proteoglycan loss and calcificatio...
Source: J Cell Mol Med - February 12, 2024 Category: Molecular Biology Authors: Jinyi Zhou Jinting Wu Fangda Fu Sai Yao Wenbiao Zheng Weibin Du Huan Luo Hongting Jin Peijian Tong Chengliang Wu Hongfeng Ruan Source Type: research
Genetic justification of COVID-19 patient outcomes using DERGA, a novel data ensemble refinement greedy algorithm
This study aims to identify crucial complement-related variants and determine an optimal pattern for accurate disease outcome prediction. Genetic data from 204 COVID-19 patients hospitalized between April 2020 and April 2021 at three referral centres were analysed using an artificial intelligence-based algorithm to predict disease outcome (ICU vs. non-ICU admission). A recently introduced alpha-index identified the 30 most predictive genetic variants. DERGA algorithm, which employs multiple classification algorithms, determined the optimal pattern of these key variants, resulting in 97% accuracy for predicting disease outc...
Source: J Cell Mol Med - February 10, 2024 Category: Molecular Biology Authors: Panagiotis G Asteris Amir H Gandomi Danial J Armaghani Markos Z Tsoukalas Eleni Gavriilaki Gloria Gerber Gerasimos Konstantakatos Athanasia D Skentou Leonidas Triantafyllidis Nikolaos Kotsiou Evan Braunstein Hang Chen Robert Brodsky Tasoula Touloumenidou Source Type: research
Retraction: Ectopic expression of ROR1 prevents cochlear hair cell loss in guinea pigs with noise-induced hearing loss
J Cell Mol Med. 2024 Feb;28(4):e18169. doi: 10.1111/jcmm.18169.NO ABSTRACTPMID:38339763 | PMC:PMC10863977 | DOI:10.1111/jcmm.18169 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 10, 2024 Category: Molecular Biology Source Type: research
Methyltransferase-like 3 mediated RNA m < sup > 6 < /sup > A modifications in the reproductive system: Potentials for diagnosis and therapy
J Cell Mol Med. 2024 Feb;28(4):e18128. doi: 10.1111/jcmm.18128.ABSTRACTSeveral studies have highlighted the functional indispensability of methyltransferase-like 3 (METTL3) in the reproductive system. However, a review that comprehensively interprets these studies and elucidates their relationships is lacking. Therefore, the present work aimed to review studies that have investigated the functions of METTL3 in the reproductive system (including spermatogenesis, follicle development, gametogenesis, reproductive cancer, asthenozoospermia and assisted reproduction failure). This review suggests that METTL3 functions not only ...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Xiaojuan Su Ruifeng Lu Yi Qu Dezhi Mu Source Type: research
Tension regulates the cartilage phenotypic expression of endplate chondrocytes through the α-catenin/actin skeleton/Hippo pathway
J Cell Mol Med. 2024 Feb;28(4):e18133. doi: 10.1111/jcmm.18133.ABSTRACTThe study aimed to investigate the regulatory mechanism of intracellular tension signaling in endplate chondrocytes and its impact on extracellular matrix synthesis. Human endplate chondrocytes were subjected to tension load using Flexcell FX-5000™, and changes in phenotype, morphology, and the expression of Hippo signaling pathway and α-Catenin were assessed through various techniques. Through the overexpression of YAP and inhibition of α-Catenin, the study clarified the intracellular tension signaling pathway and its regulation of extracellular ma...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Min Zhang Shouliang Xiong Daokuan Gao Chen Liu Liang Xiao Source Type: research
Temperature-sensitive hydrogel releasing pectolinarin facilitate scarless wound healing
In this study, we successfully developed a temperature-sensitive Pluronic@F-127 hydrogel as a platform for delivering pectolinarin to promote scarless wound healing. The bioactive pectolinarin was released from the hydrogel, effectively enhancing endothelial cell migration, proliferation and the expression of angiogenesis-related genes. Additionally, a concentration of 20 μg/mL of pectolinarin demonstrated remarkable antioxidant ability, capable of counteracting the detrimental effects of reactive oxygen species (ROS). Our results from rat wound healing models demonstrated that the hydrogel accelerated wound healing, prom...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Xiaohang Chen Haoyue Song Kun Song Yuan Zhang Jia Wang Jinjia Hong Qingpeng Xie Jing Zhao Meixian Liu Xing Wang Source Type: research
Livin is protective in UVB-induced skin photodamage by regulating keratinocyte activation and inflammatory responses
J Cell Mol Med. 2024 Feb;28(4):e18124. doi: 10.1111/jcmm.18124.ABSTRACTUVB radiation can lead to skin photodamage, which might arise from keratinocyte (KC) activation. Nuclear factor kappa B (NF-κB) assumes an essential function in the context of UVB-triggered skin photodamage. Initiating the NF-κB cascade leads to the release of inflammatory factors from KCs. Livin can modulate both KC activation and function, yet it remains uncertain whether and how Livin regulates KC activation induced by UVB. To explore the involvement of Livin in UVB-triggered skin photodamage and its impact on skin damage through NF-κB activation....
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Kaijie Wang Xiaolan You Zhenri Qu Delu Che Xianwei Cao Source Type: research
Adenine base editor-based correction of the cardiac pathogenic Lmna c.1621C & gt; T mutation in murine hearts
In this study, we harnessed adeno-associated virus (AAV) and a mouse model carrying the cardiomyopathy-causing Lmna c.1621C > T mutation to demonstrate key steps and concerns in designing a cardiac ABE experiment in vivo. We found DeepABE as a reliable deep-learning-based model to predict ABE editing outcomes in the heart. Screening of sgRNAs for a Cas9 mutant with relieved protospacer adjacent motif (PAM) allowed the reduction of bystander editing. The ABE editing efficiency can be significantly enhanced by modifying the TadA and Cas9 variants, which are core components of ABEs. The ABE systems can be delivered into th...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Luzi Yang Zhanzhao Liu Jinhuan Sun Zhan Chen Fei Gao Yuxuan Guo Source Type: research
Systemic inflammation and its relationship with pruritus in early-stage mycosis fungoides
This study provides evidence of enhanced systemic inflammation in early-stage MF patients. Additionally, the correlation between pruritus with mSWAT scores and systemic inflammation parameters suggests a potential link between pruritus and the inflammatory milieu in MF.PMID:38332520 | PMC:PMC10853580 | DOI:10.1111/jcmm.18125 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Berna Solak Rabia Öztaş Kara Source Type: research
Effect of fentanyl and remifentanil on neuron damage and oxidative stress during induction neurotoxicity
J Cell Mol Med. 2024 Feb;28(4):e18118. doi: 10.1111/jcmm.18118.ABSTRACTOpioids can be used for medical and non-medical purposes. Chronic pain such as cancer, as well as the frequent use of such drugs in places such as operating rooms and intensive care units, and in non-medical areas like drug abuse the effects and side effects of these drugs need to be examined in more detail. For this purpose, the effects of fentanyl and remifentanil drugs on neuroinflammation, oxidative stress and cholinesterase metabolism were investigated. Neuron cells (CRL-10742) were used for the evaluation of the toxicity of fentanyl and remifentan...
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Ali Taghizadehghalehjoughi Muhammet Emin Naldan Yesim Yeni Sidika Genc Ahmet Hacimuftuoglu Mesut Isik Adem Necip İsmail Bolat Serkan Yildirim Sukru Beydemir Mahmut Baykan Source Type: research
ITGBL1 promotes anoikis resistance and metastasis in human gastric cancer via the AKT/FBLN2 axis
In conclusion, the findings of our study indicate that ITGBL1 may serve as a possible modulator of resistance to anoikis and the metastatic process in GC.PMID:38332530 | PMC:PMC10853594 | DOI:10.1111/jcmm.18113 (Source: J Cell Mol Med)
Source: J Cell Mol Med - February 9, 2024 Category: Molecular Biology Authors: Kanger Shen Wei Xia Kun Wang Juntao Li Wei Xu Haoran Liu Kexi Yang Jinghan Zhu Jiayu Wang Qinhua Xi Tongguo Shi Rui Li Source Type: research
