hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in a Neonatal HIE Rat Model.
In this study, we investigated how human umbilical cord mesenchymal stem cells exerted a neuroprotective effect via antiapoptotic mechanisms in a neonatal hypoxic-ischemic encephalopathy rat model. A total of 78 10-day old (P10) rats were used. After human umbilical cord mesenchymal stem cells were collected from human umbilical cords and amplified in culture, they were administered to rat subjects 1 h after induced hypoxic-ischemic encephalopathy treatment. The short-term (48 h) and long-term (28 day) outcomes were evaluated after human umbilical cord mesenchymal stem cells treatment using neurobehavioral function assessm...
Source: Cell Transplantation - September 12, 2019 Category: Cytology Authors: Xu J, Feng Z, Wang X, Xiong Y, Wang L, Ye L, Zhang H Tags: Cell Transplant Source Type: research

Research Status of Mesenchymal Stem Cells in Liver Transplantation.
This article aims to provide an overview of the current understanding of the immunomodulation achieved by the application of MSCs in liver transplantation, to discuss the problems that may be encountered when using MSCs in clinical practice, and to describe some of the underlying capabilities of MSCs in liver transplantation. Cell-cell contact, soluble molecules, and exosomes have been suggested to be critical approaches to MSCs' immunoregulation in vitro; however, the exact mechanism, especially in vivo, is still unclear. In recent years, the clinical safety of MSCs has been proven by a series of clinical trials. The obst...
Source: Cell Transplantation - September 12, 2019 Category: Cytology Authors: You Y, Wen DG, Gong JP, Liu ZJ Tags: Cell Transplant Source Type: research

Comparing the Effects of the mTOR Inhibitors Azithromycin and Rapamycin on In Vitro Expanded Regulatory T Cells.
In this study we investigated the effects of Azithromycin, compared with Rapamycin, on Treg phenotype, growth, and function when expanding bulk, naïve, and memory Tregs. Furthermore, the intracellular concentration of Rapamycin in CD4+ T cells as well as in the culture medium was measured for up to 48 h after supplemented. Treg phenotype was assessed by flow cytometry and Treg function was measured as inhibition of responder T-cell expansion in a suppression assay. The concentration of Rapamycin was quantified with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Azithromycin and Rapamycin both pr...
Source: Cell Transplantation - September 12, 2019 Category: Cytology Authors: Bergström M, Müller M, Karlsson M, Scholz H, Vethe NT, Korsgren O Tags: Cell Transplant Source Type: research

The Gut Microbiota in Multiple Sclerosis: An Overview of Clinical Trials.
In conclusion, the restoration of the microbial population in patients with RR-MS appears to reduce inflammatory events and the reactivation of the immune system. PMID: 31512505 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - September 12, 2019 Category: Cytology Authors: Schepici G, Silvestro S, Bramanti P, Mazzon E Tags: Cell Transplant Source Type: research

A circular RNA derived from MMP9 facilitates oral squamous cell carcinoma metastasis through regulation of MMP9 mRNA stability.
In this study, we identified a novel metastasis-associated circular RNA, circular matrix metalloproteinase 9 (hsa_circ_0001162, a circular RNA derived from matrix metalloproteinase 9), which was remarkably upregulated in oral squamous cell carcinoma and positively correlated with matrix metalloproteinase 9 expression. Patients with high circular matrix metalloproteinase 9 expression were prone to lymph node metastasis and an advanced TNM stage. Importantly, circular matrix metalloproteinase 9 was identified as an efficacious diagnostic and prognostic biomarker for oral squamous cell carcinoma patients. Functional experimen...
Source: Cell Transplantation - September 11, 2019 Category: Cytology Authors: Xia B, Hong T, He X, Hu X, Gao Y Tags: Cell Transplant Source Type: research

Rat Facial Nerve Regeneration with Human Immature Dental Pulp Stem Cells.
Abstract Facial paralysis can result in severe implications for the patients. However, stem cell biology has become an important field in regenerative medicine since the discovery and characterization of mesenchymal stem cells. Our aim was to evaluate the regeneration after facial nerve crush injury and application of human immature dental pulp stem cells (iDPSC). For this study 70 Wistar rats underwent a unilateral facial nerve crush injury and were divided into two groups: Group I (GI): Crushed; Group II (GII): Crushed and iDPSC, and distributed into study periods of 3, 7, 14, 21, and 42 postoperative days. Faci...
Source: Cell Transplantation - August 28, 2019 Category: Cytology Authors: Saez DM, Sasaki RT, Martins DO, Chacur M, Kerkis I, da Silva MCP Tags: Cell Transplant Source Type: research

Preservation Solutions for Kidney Transplantation: History, Advances and Mechanisms.
Abstract Solid organ transplantation was one of the greatest medical advances during the past few decades. Organ preservation solutions have been applied to diminish ischemic/hypoxic injury during cold storage and improve graft survival. In this article, we provide a general review of the history and advances of preservation solutions for kidney transplantation. Key components of commonly used solutions are listed, and effective supplementations for current available preservation solutions are discussed. At cellular and molecular levels, further insights were provided into the pathophysiological mechanisms of effe...
Source: Cell Transplantation - August 26, 2019 Category: Cytology Authors: Chen Y, Shi J, Xia TC, Xu R, He X, Xia Y Tags: Cell Transplant Source Type: research

Production, Characterization, and Function of Pseudoislets from Perinatal Canine Pancreas.
Abstract We evaluated the cell composition and function of canine pancreatic pseudoislets (PIs) produced from 42- to 55-day-old fetuses, 1- to 21-day-old pups, and an adult dog pancreas. After mild collagenase treatment, partially digested tissues were cultured for 2-3 weeks. PI production started on culture day 3, was marked for 6 to 9 days, and then stopped. PI production was greatest with the neonatal specimens, reaching about 12 million aggregates per litter (55-day-old fetus) or per pancreas (1-day-old pup). Cell composition at all stages was similar to that in adult pancreatic islets, with predominant β...
Source: Cell Transplantation - August 26, 2019 Category: Cytology Authors: Czernichow P, Reynaud K, Kerr-Conte J, Furthner E, Ravassard P Tags: Cell Transplant Source Type: research

Exosomes from Bone Marrow Mesenchymal Stem Cells Inhibit Neuronal Apoptosis and Promote Motor Function Recovery via the Wnt/ β-catenin Signaling Pathway.
In this study, we designed SCI models in vivo and in vitro and then investigated the possible mechanism of successful repair by BMSCs-Exos. In vivo, we established one Sham group and two SCI model groups. The Basso, Beattie, Bresnahan (BBB) scores showed that BMSCs-Exos could effectively promote the recovery of spinal cord function. The results of the Nissl staining, immunohistochemistry, and TUNEL/NeuN/DAPI double staining showed that BMSCs-Exos inhibited neuronal apoptosis. Western blot analysis showed that the protein expression level of Bcl-2 was significantly increased in the BMSCs-Exos group compared with the PBS gro...
Source: Cell Transplantation - August 19, 2019 Category: Cytology Authors: Li C, Jiao G, Wu W, Wang H, Ren S, Zhang L, Zhou H, Liu H, Chen Y Tags: Cell Transplant Source Type: research

Interspecies Organogenesis for Human Transplantation.
H, Steer CJ, Low WC Abstract Blastocyst complementation combined with gene editing is an emerging approach in the field of regenerative medicine that could potentially solve the worldwide problem of organ shortages for transplantation. In theory, blastocyst complementation can generate fully functional human organs or tissues, grown within genetically engineered livestock animals. Targeted deletion of a specific gene(s) using gene editing to cause deficiencies in organ development can open a niche for human stem cells to occupy, thus generating human tissues. Within this review, we will focus on the pancreas, liv...
Source: Cell Transplantation - August 19, 2019 Category: Cytology Authors: Crane AT, Aravalli RN, Asakura A, Grande AW, Krishna VD, Carlson DF, Cheeran MC, Danczyk G, Dutton JR, Hackett PB, Hu WS, Li L, Lu WC, Miller ZD, O'Brien TD, Panoskaltsis-Mortari A, Parr AM, Pearce C, Ruiz-Estevez M, Shiao M, Sipe CJ, Toman NG, Voth J, Xi Tags: Cell Transplant Source Type: research

αCGRP affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway.
αCGRP affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway. Cell Transplant. 2019 Aug 19;:963689719871000 Authors: Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L Abstract Alpha-calcitonin gene-related peptide (αCGRP) plays a significant pathophysiological role in the regulation of bone metabolism. Our previous research indicated that αCGRP might have a potential application in enhancing osseointegration in vivo. To further uncover the intrinsic mechanism of its networks in bone regeneration, here we investigate the impact of αCGRP on osteogenic differenti...
Source: Cell Transplantation - August 19, 2019 Category: Cytology Authors: Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L Tags: Cell Transplant Source Type: research

Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies.
We describe an isolation method, characterize a population, and investigate its potential for cell banking and thus suitability as a potential product for cell grafting therapies. Our results show hFDF and a bone marrow-derived mesenchymal stem cell (BM-MSC) line shared identification markers and in vitro multilineage differentiation potential into osteogenic, chondrogenic, and adipogenic lineages. The hFDF population exhibited similar cell characteristics as BM-MSC while producing lower pro-inflammatory cytokine IL-6 levels and higher levels of the wound healing factor hepatocyte growth factor. We demonstrate in vitro dif...
Source: Cell Transplantation - August 13, 2019 Category: Cytology Authors: Esteban-Vives R, Ziembicki J, Choi MS, Thompson RL, Schmelzer E, Gerlach JC Tags: Cell Transplant Source Type: research

Progenitor/Stem Cell Delivery by Suprarenal Aorta Route in Acute Kidney Injury.
re JM Abstract Progenitor/stem cell-based kidney regenerative strategies are a key step towards the development of novel therapeutic regimens for kidney disease treatment. However, the route of cell delivery, e.g., intravenous, intra-arterial, or intra-parenchymal, may affect the efficiency for kidney repair in different models of acute and chronic injury. Here, we describe a protocol of intra-aorta progenitor/stem cell injection in rats following either acute ischemia-reperfusion injury or acute proteinuria induced by puromycin aminonucleoside (PAN) - the experimental prototype of human minimal change disease and...
Source: Cell Transplantation - August 13, 2019 Category: Cytology Authors: Rangel ÉB, Gomes SA, Kanashiro-Takeuchi R, Hare JM Tags: Cell Transplant Source Type: research

The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats are Possibly Mediated by Preventing Blood-Brain Barrier Disruption and RIP3-Mediated Necroptosis.
This study aims to reveal the role of necroptosis after SAH, and explore whether the disruption of the blood-brain barrier (BBB) and RIP3-mediated necroptosis following SAH in a rat SAH model are altered by necrostatin-1 via its selective inhibition of receptor-interacting protein kinase 1 (RIP1). Sixty-five rats were used in the experiments. The SAH model was established using endovascular perforation. Necrostatin-1 was intracerebroventricularly injected 1 h before SAH induction. The neuroprotective effects of necrostatin-1 were evaluated with multiple methods such as magnetic resonance imaging (MRI) scanning, immunohisto...
Source: Cell Transplantation - August 2, 2019 Category: Cytology Authors: Chen J, Jin H, Xu H, Peng Y, Jie L, Xu D, Chen L, Li T, Fan L, He P, Ying G, Gu C, Wang C, Wang L, Chen G Tags: Cell Transplant Source Type: research

Survival Rates of Various Autologous Chondrocyte Grafts and Concomitant Procedures. A Prospective Single-Center Study over 18 Years.
Abstract Seven different autologous chondrocyte implantation (ACI) grafts were used consecutively over a period of 18 years for the treatment of cartilage lesions in the knees. The aim was to evaluate this entire ACI patient series for graft-related or unrelated serious adverse events (SAE), graft failures, and to reveal potential risk factors for these incidents. The study group comprised 151 operated patients: classical periosteum-ACI (n = 45); ACI-seeded fibrin-collagen patch, fixed by either periosteum (n = 59), collagen membrane (n = 15), or fibrin glue (n = 6); ACI seeded alginate-agarose hydrogel (n = 14); ...
Source: Cell Transplantation - August 2, 2019 Category: Cytology Authors: Martinčič D, Mekač J, Drobnič M Tags: Cell Transplant Source Type: research

The Morphological Analysis of Cells in the Peritoneal Washing Fluids of Patients with Gastric Cancer.
Abstract The cytology of peritoneal washing fluids for gastric cancer is the most basic method for judging peritoneal micrometastasis. However, the clinical value of this method is not clear at present. A retrospective analysis was performed on 277 patients with pathologically proven and surgically treated gastric cancer. The peritoneal washing fluids were collected after opening the abdomen and before the operation, and were sent to the cytology laboratory for screening of occult cancer cells in the collected washing fluids. The number of cases diagnosed as cancer cells, reactive mesothelial cells, serosal balls,...
Source: Cell Transplantation - August 1, 2019 Category: Cytology Authors: Jiang RP, Xiong XJ, Qiu XS, Wang EH, Wu GP Tags: Cell Transplant Source Type: research

Fewer Islets Survive from a First Transplant than a Second Transplant: Evaluation of Repeated Intraportal Islet Transplantation in Mice.
This study aimed to investigate whether there are differences in islet survival and engraftment between a first and a second transplantation, performed 1 week apart, to the liver. C57BL/6 mice were accordingly transplanted twice with an initial infusion of syngeneic islets expressing green fluorescent protein (GFP). The second islet transplant was performed 1 week later and consisted of islets isolated from non-GFP C57BL/6-mice. Animals were sacrificed either 1 day or 1 month after the second transplantation. A control group received a saline infusion instead of GFP-expressing islets, 1 week later obtained a standard non-G...
Source: Cell Transplantation - July 30, 2019 Category: Cytology Authors: Liljebäck H, Quach M, Carlsson PO, Lau J Tags: Cell Transplant Source Type: research

Neural Repair in Stroke.
This article reviews the progress that has been made in the development of cell therapies for the repair of nervous system damage caused by strokes, since the first report on the use of cell transplants in animal models of ischemic brain injury in 1988. At that time neural progenitor cells derived from fetal brain tissue were used as sources of cells to replace specific subsets of neuronal cells that were lost in various regions of the brain following experimentally induced strokes. Since 1988, cells from other sources, such as embryonic stem cells and inducible pluripotent stem cells, have been investigated for their abil...
Source: Cell Transplantation - July 29, 2019 Category: Cytology Authors: Toman NG, Grande AW, Low WC Tags: Cell Transplant Source Type: research

Chondrocyte Co-cultures with the Stromal Vascular Fraction of Adipose Tissue in Polyhydroxybutyrate/Poly-(hydroxybutyrate-co-hydroxyhexanoate) Scaffolds: Evaluation of Cartilage Repair in Rabbit.
In this study, the cellular morphologies and proliferation capabilities on scaffolds were evaluated. Next, scaffolds with 1:1 co-culture of ASCs/SVF and chondrocytes were implanted into the full-thickness cartilage defects in rabbit knee for 10 weeks. Cells seeded on the scaffolds showed better adhesion, migration, and proliferation in vitro. Importantly, implantation with scaffolds with SVF and chondrocytes revealed more desirable in vivo healing outcomes. Our results illustrate a one-step surgical procedure for the regeneration of focal cartilage defects using a mixture of SVF from adipose tissue and uncultured chondrocy...
Source: Cell Transplantation - July 24, 2019 Category: Cytology Authors: Ba K, Wei X, Ni D, Li N, Du T, Wang X, Pan W Tags: Cell Transplant Source Type: research

Additional Use of Synovial Mesenchymal Stem Cell Transplantation Following Surgical Repair of a Complex Degenerative Tear of the Medial Meniscus of the Knee: A Case Report.
Abstract Complex degenerative tears of the medial meniscus in the knee are usually treated using meniscectomy. However, this procedure increases the risk of osteoarthritis, while other treatments aimed at meniscal repair remain challenging due to the high possibility of failure. The use of synovial mesenchymal stem cells (MSCs) is an attractive additional approach for meniscal repair, as these cells have high proliferative and chondrogenic potential. In this case report, we surgically repaired a complex degenerative tear of the medial meniscus and then transplanted autologous synovial MSCs. We evaluated clinical o...
Source: Cell Transplantation - July 17, 2019 Category: Cytology Authors: Sekiya I, Koga H, Otabe K, Nakagawa Y, Katano H, Ozeki N, Mizuno M, Horie M, Kohno Y, Katagiri K, Watanabe N, Muneta T Tags: Cell Transplant Source Type: research

Nanomedicine-based Curcumin Approach Improved ROS Damage in Best Dystrophy-specific Induced Pluripotent Stem Cells.
In this study, we developed patient-specific induced pluripotent stem cells (BD-iPSCs) from BD patient-derived dental pulp stromal cells and then differentiated BD-iPSCs into retinal pigment epithelial cells (BD-RPEs). BD-RPEs were used as an expandable platform for in vitro candidate drug screening. Compared with unaffected sibling-derived iPSC-derived RPE cells (Ctrl-RPEs), BD-RPEs exhibited typical RPE-specific markers with a lower expression of the tight junction protein ZO-1 and Bestrophin-1 (BEST1), as well as reduced phagocytic capabilities. Notably, among all candidate drugs, curcumin was the most effective for upr...
Source: Cell Transplantation - July 17, 2019 Category: Cytology Authors: Lin TC, Lin YY, Hsu CC, Yang YP, Yang CH, Hwang DK, Wang CY, Liu YY, Lo WL, Chiou SH, Peng CH, Chen SJ, Chang YL Tags: Cell Transplant Source Type: research

Neurotrophic Factor Secretion and Neural Differentiation Potential of Multilineage-differentiating Stress-enduring (Muse) Cells Derived from Mouse Adipose Tissue.
Abstract Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent stem cells that can be isolated based on stage-specific embryonic antigen-3 (SSEA-3), a pluripotent stem cell-surface marker. However, their capacities for survival, neurotrophic factor secretion, and neuronal and glial differentiation are unclear in rodents. Here we analyzed mouse adipose tissue-derived Muse cells in vitro. We collected mesenchymal stem cells (MSCs) from C57BL/6 J mouse adipose tissue and separated SSEA-3+, namely Muse cells, and SSEA-3-, non-Muse cells, to assess self-renewability; pluripotency marker e...
Source: Cell Transplantation - July 15, 2019 Category: Cytology Authors: Nitobe Y, Nagaoki T, Kumagai G, Sasaki A, Liu X, Fujita T, Fukutoku T, Wada K, Tanaka T, Kudo H, Asari T, Furukawa KI, Ishibashi Y Tags: Cell Transplant Source Type: research

D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain.
Gajović S Abstract Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSC) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSC transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSC better than the uncoated nanoparticles...
Source: Cell Transplantation - July 11, 2019 Category: Cytology Authors: Pongrac IM, Radmilović MD, Ahmed LB, Mlinarić H, Regul J, Škokić S, Babič M, Horák D, Hoehn M, Gajović S Tags: Cell Transplant Source Type: research

Collagen VII Expression is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Formation in Bilayer Engineered Skin Substitutes.
Abstract The blistering disease Recessive Dystrophic Epidermolysis Bullosa (RDEB) is caused by mutations in the gene encoding collagen VII (COL7), which forms anchoring fibrils that attach the epidermis to the dermis. Cutaneous gene therapy to restore COL7 expression in RDEB patient cells has been proposed, and cultured epithelial autograft containing COL7-modified keratinocytes was previously tested in clinical trials. Because COL7 in normal skin is expressed in both fibroblasts and keratinocytes, cutaneous gene therapy using a bilayer skin substitute may enable faster restoration of anchoring fibrils. Hypothetic...
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Supp DM, Hahn JM, Combs KA, McFarland KL, Schwentker A, Boissy RE, Boyce ST, Powell HM, Lucky AW Tags: Cell Transplant Source Type: research

Neural Stem Cell Transplantation Improves Locomotor Function in Spinal Cord Transection Rats Associated with Nerve Regeneration and IGF-1 R Expression.
Abstract Transplantation of neural stem cells (NSCs) is a potential strategy for the treatment of spinal cord transection (SCT). Here we investigated whether transplanted NSCs would improve motor function of rats with SCT and explored the underlying mechanism. First, the rats were divided into sham, SCT, and NSC groups. Rats in the SCT and NSC groups were all subjected to SCT in T10, and were administered with media and NSC transplantation into the lesion site, respectively. Immunohistochemistry was used to label Nestin-, TUNEL-, and NeuN-positive cells and reveal the expression and location of type I insulin-like...
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Zhao XM, He XY, Liu J, Xu Y, Xu FF, Tan YX, Zhang ZB, Wang TH Tags: Cell Transplant Source Type: research

A Case Series on Natural Conceptions Resulting in Ongoing Pregnancies in Menopausal and Prematurely Menopausal Women Following Platelet-Rich Plasma Treatment.
Abstract Since the introduction of autologous platelet-rich plasma (PRP) in medical practice, various studies have documented that implementing PRP can enhance healing and the anti-aging process, employing angiogenesis regeneration due to the multiple growth factors and cytokines involved. Numerous reports have shown promising results with the use of PRP in ovarian treatment, regarding ovarian regeneration and reactivation of folliculogenesis. This case series reports on two women with premature ovarian failure (POF) aged 40 and 27 years, respectively, and one menopausal woman aged 46 years. All patients presented...
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Pantos K, Simopoulou M, Pantou A, Rapani A, Tsioulou P, Nitsos N, Syrkos S, Pappas A, Koutsilieris M, Sfakianoudis K Tags: Cell Transplant Source Type: research

Reconstruction of the Damaged Dorsal Root Entry Zone by Transplantation of Olfactory Ensheathing Cells.
In this study, we compared short- and long-term OEC survival and their interaction with the surrounding dorsal root tissue. We reveal how transplanted OECs orient toward the spinal cord and allow newly formed axons to travel across into the dorsal horn of the spinal cord. Reconstruction of the dorsal root entry zone was supported by OEC ensheathment of axons at the injured site and also at around 3 mm further away at the dorsal root ganglion. Quantitative analysis revealed no observable difference in dorsal column axonal loss between transplanted and control groups of rats. PMID: 31271055 [PubMed - as supplied by publ...
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Collins A, Ibrahim A, Li D, Liadi M, Li Y Tags: Cell Transplant Source Type: research

miR-145 Contributes to the Progression of Cervical Carcinoma by Directly Regulating FSCN1.
In this study, quantitative real-time PCR (qRT-PCR) was used to detect miR-145 and FSCN1 expression levels in tissues and HeLa cells. Western blotting was performed to determine the protein level of FSCN1. The luciferase assay was used to verify the direct target of miR-145. The CCK-8 assay and 2D colony formation assays were performed to determine the effects of miR-145 mimics or FSCN1 silencing on cell proliferation. miR-145 expression levels were significantly down-regulated, while FSCN1 expression levels were significantly up-regulated in the cervical carcinoma tissues compared with their matched non-cancerous tissues....
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Ma L, Li LL Tags: Cell Transplant Source Type: research

Harnessing Neurogenesis and Neuroplasticity with Stem Cell Treatment for Addictive Disorders.
Abstract Drug and alcohol addiction has become an emerging public health issue and is a great burden to patients, their families, and society. It is characterized by high relapse rates and significant morbidity and mortality, and most available treatments result in only modest improvement. These findings highlight the necessity for new approaches to treat addiction. Scientific reports in the past two decades suggest that addiction involves impaired neural plasticity and decreased hippocampal neurogenesis. Stem cell therapy and its derived neurotrophic factors can potentially target the underlying pathophysiology o...
Source: Cell Transplantation - July 4, 2019 Category: Cytology Authors: Tsai ST, Liew HK, Li HM, Lin SZ, Chen SY Tags: Cell Transplant Source Type: research

B Lymphocytes are the Target of Mesenchymal Stem Cells Immunoregulatory Effect in a Murine Graft-versus-Host Disease Model.
This study may provide a mechanistic clue for the treatment of human clinical aGvHD. PMID: 31257911 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - July 1, 2019 Category: Cytology Authors: Lu D, Ma T, Zhou X, Jiang Y, Han Y, Li H Tags: Cell Transplant Source Type: research

Metabolic Syndrome Induces Release of Smaller Extracellular Vesicles from Porcine Mesenchymal Stem Cells.
This study tested the hypothesis that MetS shifts the size distribution of MSC-derived EV. Adipose tissue-derived MSC-EV subpopulations from Lean (n = 6) and MetS (n = 6) pigs were characterized for number and size using nanoparticle-tracking analysis, flow cytometry, and transmission electron microscopy. Expression of exosomal genes was determined using next-generation RNA-sequencing (RNA-seq). The number of EV released from Lean and MetS pig MSC was similar, yet MetS-MSC yielded a higher proportion of small-size EV (202.4 ± 17.7 nm vs. 280.3 ± 15.1 nm), consistent with exosomes. RNA-seq showed that their EV...
Source: Cell Transplantation - June 28, 2019 Category: Cytology Authors: Conley SM, Shook JE, Zhu XY, Eirin A, Jordan KL, Woollard JR, Isik B, Hickson LJ, Puranik AS, Lerman LO Tags: Cell Transplant Source Type: research

Stem Cell-Related Studies and Stem Cell-Based Therapies in Liver Diseases.
Abstract Owing to the increasing worldwide burden of liver diseases, the crucial need for safe and effective interventions for treating end-stage liver failure has been a very productive line of inquiry in the discipline of hepatology for many years. Liver transplantation is recognized as the most effective treatment for end-stage liver disease; however, the shortage of donor organs, high medical costs, and lifelong use of immunosuppressive agents represent major drawbacks and demand exploration for alternative treatments. Stem cell-based therapies have been widely studied in the field of liver diseases and are co...
Source: Cell Transplantation - June 26, 2019 Category: Cytology Authors: Zhou W, Nelson ED, Abu Rmilah AA, Amiot BP, Nyberg SL Tags: Cell Transplant Source Type: research

Cells Involved in Urethral Tissue Engineering: Systematic Review.
Abstract The urethra is part of the lower urinary tract and its main role is urine voiding. Its complex histological structure makes urethral tissue prone to various injuries with complicated healing processes that often lead to scar formation. Urethral stricture disease can affect both men and women. The occurrence of this pathology is more common in men and thus are previous research has been mainly oriented on male urethra reconstruction. However, commonly used surgical techniques show unsatisfactory results because of complications. The new and progressively developing field of tissue engineering offers promis...
Source: Cell Transplantation - June 25, 2019 Category: Cytology Authors: Culenova M, Ziaran S, Danisovic L Tags: Cell Transplant Source Type: research

MicroRNA-101a Regulates Autophagy Phenomenon via the MAPK Pathway to Modulate Alzheimer's-Associated Pathogenesis.
This study found that the autophagy phenomenon regulated by miRNA-101a via the MAPK pathway might be a new mechanism in AD. This could provide new insights into AD formation and treatment. PMID: 31204500 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - June 17, 2019 Category: Cytology Authors: Li Q, Wang Y, Peng W, Jia Y, Tang J, Li W, Zhang JH, Yang J Tags: Cell Transplant Source Type: research

The Impact of Limbal Mesenchymal Stromal Cells on Healing of Acute Ocular Surface Wounds is Improved by Pre-cultivation and Implantation in the Presence of Limbal Epithelial Cells.
Abstract While limbal epithelial cells are used for treating ocular surface wounds, the therapeutic potential of mesenchymal cells cultivated from the limbal stroma (LMSC) is less clear. We have therefore examined the effects of LMSC when applied to acute ocular surface wounds. LMSC derived from male rabbits (RLMSC) were applied to the ocular surface of female rabbits immediately following removal of the corneal and limbal epithelium. Human amniotic membrane (HAM) was used as the vehicle for implanting the RLMSC. The effects of RLMSC were examined when applied alone (n = 3) and in conjunction with a stratified cul...
Source: Cell Transplantation - June 17, 2019 Category: Cytology Authors: Nili E, Li FJ, Dawson RA, Lau C, McEwan B, Barnett NL, Weier S, Walshe J, Richardson NA, Harkin DG Tags: Cell Transplant Source Type: research

CD200Fc Improves Neurological Function by Protecting the Blood-brain Barrier after Intracerebral Hemorrhage.
In conclusion, our work demonstrated that CD200Fc might be a potential treatment option for ICH by protecting the BBB and improving functional outcomes. PMID: 31208229 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - June 17, 2019 Category: Cytology Authors: Le CS, Hao XD, Li JW, Zhong JW, Lin HR, Zhou YT, Travis ZD, Tong LS, Gao F Tags: Cell Transplant Source Type: research

Initiating TrkB/Akt Signaling Cascade Preserves Blood-Brain Barrier after Subarachnoid Hemorrhage in Rats.
This study aimed to investigate activation of the Tropomyosin-related kinase receptor B (TrkB) and its downstream signaling pathway on preserving BBB breakdown after experimental SAH. An endovascular perforation SAH model was applied. N-[2-(5-hydroxy-1H-indol-3-yl) ethyl]-2- oxopiperidine-3-carboxamide (HIOC), the derivative of N-acetyl serotonin (NAS), was intracerebroventricularly administered 3 h after SAH induction. The neurologic scores and brain water content were evaluated in an outcome study. Western blot and immunofluorescence staining were used to investigate the mechanism. The results indicated that HIOC activat...
Source: Cell Transplantation - June 17, 2019 Category: Cytology Authors: Qi X, Liu J, Wu J, Bi Y, Han C, Zhang G, Lou M, Lu J, Tang J Tags: Cell Transplant Source Type: research

Detection of Crossed Cerebellar Diaschisis by Intravoxel Incoherent Motion MR Imaging in Subacute Ischemic Stroke.
Abstract Intravoxel incoherent motion has received extensive attention in brain studies for its potential as a non-invasive magnetic resonance perfusion method. However, studies on intravoxel incoherent motion imaging and crossed cerebellar diaschisis detection are relatively scarce. The aim of our study was to evaluate the feasibility of using intravoxel incoherent motion imaging in crossed cerebellar diaschisis diagnosis in subacute ischemic stroke patients by comparing results from intravoxel incoherent motion imaging, single-photon emission computed tomography, and arterial spin-labeling perfusion methods. In ...
Source: Cell Transplantation - June 14, 2019 Category: Cytology Authors: Wang J, Suo S, Zu J, Zhu W, Pan L, Song S, Li Y, Li L, Chen Z, Xu J Tags: Cell Transplant Source Type: research

The Efficacy of a Scaffold-free Bio 3D Conduit Developed from Autologous Dermal Fibroblasts on Peripheral Nerve Regeneration in a Canine Ulnar Nerve Injury Model: A Preclinical Proof-of-Concept Study.
Abstract Autologous nerve grafting is widely accepted as the gold standard treatment for segmental nerve defects. To overcome the inevitable disadvantages of the original method, alternative methods such as the tubulization technique have been developed. Several studies have investigated the characteristics of an ideal nerve conduit in terms of supportive cells, scaffolds, growth factors, and vascularity. Previously, we confirmed that biological scaffold-free conduits fabricated from human dermal fibroblasts promote nerve regeneration in a rat sciatic nerve injury model. The purpose of this study is to evaluate th...
Source: Cell Transplantation - June 12, 2019 Category: Cytology Authors: Mitsuzawa S, Ikeguchi R, Aoyama T, Takeuchi H, Yurie H, Oda H, Ohta S, Ushimaru M, Ito T, Tanaka M, Kunitomi Y, Tsuji M, Akieda S, Nakayama K, Matsuda S Tags: Cell Transplant Source Type: research

The neuroprotection of hypoxic adipose tissue-derived mesenchymal stem cells in experimental traumatic brain injury.
In this study, we evaluated whether hypoxic preconditioned mesenchymal stem cells could enhance the recovery from traumatic brain injury. Traumatic brain injury was induced with an electromagnetically controlled cortical impact device. Two million mesenchymal stem cells derived from the adipose tissue of transgenic green fluorescent protein Sprague-Dawley rats were cultured under either hypoxic (2.5% O2 for 18 hours) (N = 30) or normoxic (18% O2) (N = 30) conditions, then topically applied to the exposed cerebral cortex within 1 hour after traumatic brain injury. A thin layer of fibrin was used to fix the cells in position...
Source: Cell Transplantation - June 12, 2019 Category: Cytology Authors: Ma H, Lam PK, Tong CSW, Lo KKY, Wong GKC, Poon WS Tags: Cell Transplant Source Type: research

A Hallmark Clinical Study of Cord Blood Therapy in Adults with Ischemic Stroke.
Abstract The therapeutic application of human umbilical cord blood cells has been an area of great interest for at least the last 25 years. Currently, cord blood cells are approved for reconstitution of the bone marrow following myeloablation in both young and old patients with myeloid malignancies and other blood cancers. Translational studies investigating alternative uses of cord blood have also shown that these cells not only stimulate neurogenesis in the aged brain but are also potentially therapeutic in the treatment of adult neurodegenerative disorders including amyotrophic lateral sclerosis, Alzheimer's di...
Source: Cell Transplantation - June 11, 2019 Category: Cytology Authors: Sanberg PR, Ehrhart J Tags: Cell Transplant Source Type: research

(-)-Phenserine Ameliorates Contusion Volume, Neuroinflammation, and Behavioral Impairments Induced by Traumatic Brain Injury in Mice.
In this study, we used a mouse model of moderate to severe TBI by controlled cortical impact to assess the effects of Phen on post-trauma histochemical and behavioral changes. Animals were treated with Phen (2.5 mg/kg, IP, BID) for 5 days started on the day of injury and the effects were evaluated by behavioral and histological examinations at 1 and 2 weeks after injury. Phen significantly attenuated TBI-induced contusion volume, enlargement of the lateral ventricle, and behavioral impairments in motor asymmetry, sensorimotor functions, motor coordination, and balance functions. The morphology of microglia was shifted to a...
Source: Cell Transplantation - June 10, 2019 Category: Cytology Authors: Hsueh SC, Lecca D, Greig NH, Wang JY, Selman W, Hoffer BJ, Miller JP, Chiang YH Tags: Cell Transplant Source Type: research

Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke.
This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia-reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days afte...
Source: Cell Transplantation - June 4, 2019 Category: Cytology Authors: Sibov TT, Pavon LF, Cabral FR, Cunha IF, de Oliveira DM, de Souza JG, Marti LC, da Cruz EF, Malheiros JM, Paiva FF, Tannús A, de Oliveira SM, da Costa MDS, Dastoli PA, Mendonça JN, de Toledo SRC, Malheiros SMF, de Paiva Neto MA, Rego NBB, Moron AF, Cava Tags: Cell Transplant Source Type: research

Analysis of Synapses in Cerebral Organoids.
Abstract Cerebral organoids are an emerging cutting-edge technology to model human brain development and neurodevelopmental disorders, for which mouse models exhibit significant limitations. In the human brain, synaptic connections define neural circuits, and synaptic deficits account for various neurodevelopmental disorders. Thus, harnessing the full power of cerebral organoids for human brain modeling requires the ability to visualize and analyze synapses in cerebral organoids. Previously, we devised an optimized method to generate human cerebral organoids, and showed that optimal organoids express mature-neuron...
Source: Cell Transplantation - June 4, 2019 Category: Cytology Authors: Yakoub AM, Sadek M Tags: Cell Transplant Source Type: research

Pancreatic Stellate Cells Facilitate Perineural Invasion of Pancreatic Cancer via HGF/c-Met Pathway.
In conclusion, PSCs facilitate PC cell PNI via the HGF/c-Met pathway. Targeting the HGF/c-Met signaling pathway could be a promising therapeutic strategy for PC. PMID: 31161784 [PubMed - as supplied by publisher] (Source: Cell Transplantation)
Source: Cell Transplantation - June 4, 2019 Category: Cytology Authors: Nan L, Qin T, Xiao Y, Qian W, Li J, Wang Z, Ma J, Ma Q, Wu Z Tags: Cell Transplant Source Type: research

Adipose-Derived Neural Stem Cells Combined with Acellular Dermal Matrix as a Neural Conduit Enhances Peripheral Nerve Repair.
Abstract Reconstruction to close a peripheral nerve gap continues to be a challenge for clinical medicine, and much effort is being made to develop nerve conduits facilitate nerve gap closure. Acellular dermal matrix (ADM) is mainly used to aid wound healing, but its malleability and plasticity potentially enable it to be used in the treatment of nerve gaps. Adipose-derived stem cells (ADSCs) can be differentiated into three germ layer cells, including neurospheres. We tested the ability of ADSC-derived neural stem cells (NSCs) in combination with ADM or acellular sciatic nerve (ASN) to repair a transected sciatic...
Source: Cell Transplantation - May 31, 2019 Category: Cytology Authors: Syu WZ, Hueng DY, Chen WL, Chan JY, Chen SG, Huang SM Tags: Cell Transplant Source Type: research

TGF- β in Mice Ameliorates Experimental Autoimmune Encephalomyelitis in Regulating NK Cell Activity.
TGF-β in Mice Ameliorates Experimental Autoimmune Encephalomyelitis in Regulating NK Cell Activity. Cell Transplant. 2019 May 29;:963689719852354 Authors: Xu J, Wang Y, Jiang H, Sun M, Gao J, Xie A Abstract Multiple sclerosis is a disease characterized by inflammation and demyelination located in the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is the most common animal model for multiple sclerosis (MS). Although the roles of T cells in MS/EAE have been well investigated, little is known about the functions of other immune cells in the neuroinflammation model. Here we f...
Source: Cell Transplantation - May 29, 2019 Category: Cytology Authors: Xu J, Wang Y, Jiang H, Sun M, Gao J, Xie A Tags: Cell Transplant Source Type: research

Protective Effect of Hyperbaric Oxygen Therapy on Cognitive Function in Patients with Vascular Dementia.
This article aims to reveal the protective effects and underlying mechanisms of HBO on VD. A total of 158 patients with VD were prospectively included in the study and were randomly divided into control group and HBO group. The control group was given conventional treatment and the HBO group was treated with HBO in addition to conventional treatment. The following HBO protocol was practiced: 5 days per week, 60 min each, 100% oxygen at 2 standard atmospheric pressures for 12 weeks. The Mini-Mental State Examination (MMSE) scores and serum Humanin levels were detected before and after treatments in both groups. The baseline...
Source: Cell Transplantation - May 28, 2019 Category: Cytology Authors: Xu Y, Wang Q, Qu Z, Yang J, Zhang X, Zhao Y Tags: Cell Transplant Source Type: research

Retrospective Case Series of Traumatic Brain Injury and Post-Traumatic Stress Disorder Treated with Hyperbaric Oxygen Therapy.
Abstract Returning veterans are frequently diagnosed with traumatic brain injury (TBI) and post-traumatic stress disorder (PTSD). Considering a recent case-controlled study of hyperbaric oxygen therapy (HBOT) reporting a reduction in suicidal ideation, we investigated retrospectively three veterans with chronic TBI/PTSD symptoms who were treated with multiple rounds of HBOT with neurophysiological testing performed before and after treatment. Improvements were detected on parameters within neurocognitive domains, including reductions in suicide-related symptoms. These findings independently confirm that HBOT may b...
Source: Cell Transplantation - May 28, 2019 Category: Cytology Authors: Douglas Shytle R, Eve DJ, Kim SH, Spiegel A, Sanberg PR, Borlongan CV Tags: Cell Transplant Source Type: research

Anti-Platelet Therapy in Mild Cerebral Infarction Patients on the Basis of CYP2C19 Metabolizer Status.
This study aimed to investigate the effects of CYP2C19 metabolizer status on the clinical therapeutic efficacy of cerebral infarction. Patients with cerebral infarction ( n = 180; NIHSS score ≤ 5) were recruited and divided into Group A and Group B according to CYP2C19 metabolizer status. In Group A, patients received routine clopidogrel therapy for 1 year; in Group B, the patients with extensive metabolizer (EM) were treated with clopidogrel, and patients with intermediate metabolizer (IM) and poor metabolizer (PM) were treated with aspirin for 1 year. On admission, National Institutes of Health Stroke Scale score was ...
Source: Cell Transplantation - May 28, 2019 Category: Cytology Authors: Lan H, Ying T, Xi-Hua S, Yi L Tags: Cell Transplant Source Type: research