ANGPTL4 regulates CD163 expression and Kuppfer cell polarization induced cirrhosis via TLR4/NF- κB pathway
Exp Cell Res. 2021 Jun 16:112706. doi: 10.1016/j.yexcr.2021.112706. Online ahead of print.ABSTRACTAngiopoietin like 4 (ANGPTL4) has been proved to play an important role in lipid and glucose metabolism disorders and related cardiovascular diseases, but its role in the formation of cirrhosis still needs to be further explored. Therefore, the aim of this study was to investigate the role of ANGPTL4 in the development of liver cirrhosis and its mechanism, as well as its effect on Kupffer cell polarization and hepatic stellate cell activation. The ELISA and RT-qPCR assay were used to detect the content of ANGPTL4 in serum and ...
Source: Experimental Cell Research - June 19, 2021 Category: Cytology Authors: Xin Zhang Shanshan Yuan Xin Zhang Tao Zhang Cunying Meng Kun Zhuang Shuangsuo Dang Source Type: research

PHF19 activates Hedgehog signaling and promotes tumorigenesis in hepatocellular carcinoma
In this study, we show that the short isoform of PHD finger protein 19 (PHF19) interacts with β-TrCP, the E3 ligase of Gli1, and that knocking down PHF19 promotes the ubiquitination of Gli1. In a biological function study, PHF19 was found to promote the growth of HCC cells both in liquid culture and in soft agar. Moreover, knocking out PHF19 in a HCC mouse model (MycF/F) using the hydrodynamic method inhibited tumorigenesis and improved survival. Taken together, these results demonstrate that PHF19 promotes the growth of HCC cells by activating the Hedgehog signaling pathway.PMID:34129846 | DOI:10.1016/j.yexcr.2021.11...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Shi Xiaoyun Zi Yuyuan Xing Jie Nie Yingjie Xie Qing Deng Yuezhen Xin Haiguang Source Type: research

CTPS forms the cytoophidium in zebrafish
Exp Cell Res. 2021 Jun 12:112684. doi: 10.1016/j.yexcr.2021.112684. Online ahead of print.ABSTRACTCytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoop...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Chia-Chun Chang Gerson Dierley Keppeke Christopher L Antos Min Peng Luis Eduardo Coelho Andrade Li-Ying Sung Ji-Long Liu Source Type: research

PHF19 activates Hedgehog signaling and promotes tumorigenesis in hepatocellular carcinoma
In this study, we show that the short isoform of PHD finger protein 19 (PHF19) interacts with β-TrCP, the E3 ligase of Gli1, and that knocking down PHF19 promotes the ubiquitination of Gli1. In a biological function study, PHF19 was found to promote the growth of HCC cells both in liquid culture and in soft agar. Moreover, knocking out PHF19 in a HCC mouse model (MycF/F) using the hydrodynamic method inhibited tumorigenesis and improved survival. Taken together, these results demonstrate that PHF19 promotes the growth of HCC cells by activating the Hedgehog signaling pathway.PMID:34129846 | DOI:10.1016/j.yexcr.2021.11...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Shi Xiaoyun Zi Yuyuan Xing Jie Nie Yingjie Xie Qing Deng Yuezhen Xin Haiguang Source Type: research

CTPS forms the cytoophidium in zebrafish
Exp Cell Res. 2021 Jun 12:112684. doi: 10.1016/j.yexcr.2021.112684. Online ahead of print.ABSTRACTCytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoop...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Chia-Chun Chang Gerson Dierley Keppeke Christopher L Antos Min Peng Luis Eduardo Coelho Andrade Li-Ying Sung Ji-Long Liu Source Type: research

PHF19 activates Hedgehog signaling and promotes tumorigenesis in hepatocellular carcinoma
In this study, we show that the short isoform of PHD finger protein 19 (PHF19) interacts with β-TrCP, the E3 ligase of Gli1, and that knocking down PHF19 promotes the ubiquitination of Gli1. In a biological function study, PHF19 was found to promote the growth of HCC cells both in liquid culture and in soft agar. Moreover, knocking out PHF19 in a HCC mouse model (MycF/F) using the hydrodynamic method inhibited tumorigenesis and improved survival. Taken together, these results demonstrate that PHF19 promotes the growth of HCC cells by activating the Hedgehog signaling pathway.PMID:34129846 | DOI:10.1016/j.yexcr.2021.11...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Shi Xiaoyun Zi Yuyuan Xing Jie Nie Yingjie Xie Qing Deng Yuezhen Xin Haiguang Source Type: research

CTPS forms the cytoophidium in zebrafish
Exp Cell Res. 2021 Jun 12:112684. doi: 10.1016/j.yexcr.2021.112684. Online ahead of print.ABSTRACTCytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoop...
Source: Experimental Cell Research - June 15, 2021 Category: Cytology Authors: Chia-Chun Chang Gerson Dierley Keppeke Christopher L Antos Min Peng Luis Eduardo Coelho Andrade Li-Ying Sung Ji-Long Liu Source Type: research

Depletion of Fractalkine ameliorates renal injury and Treg cell apoptosis via the p38MAPK pathway in lupus-prone mice
Exp Cell Res. 2021 Jun 11:112704. doi: 10.1016/j.yexcr.2021.112704. Online ahead of print.ABSTRACTFractalkine (FKN) is a chemokine with several roles, including chemotaxis; adhesion; and immune damage, which also participates in cell inflammation and apoptosis and responds to the pathogenesis of autoimmune diseases. Given the involvement of regulatory T cells (Treg) cells in autoimmune diseases, this study investigated the regulatory mechanism of FKN in renal injury and Treg apoptosis via the p38 mitogen-activated protein kinase (p38MAPK) signaling pathway in lupus-prone mice. Lupus was induced in BALB/c female mice by inj...
Source: Experimental Cell Research - June 14, 2021 Category: Cytology Authors: Jingxue Ma Qiming Gong Xiuhong Pan Pengwei Guo Linlin He Yanwu You Source Type: research

Depletion of Fractalkine ameliorates renal injury and Treg cell apoptosis via the p38MAPK pathway in lupus-prone mice
Exp Cell Res. 2021 Jun 11:112704. doi: 10.1016/j.yexcr.2021.112704. Online ahead of print.ABSTRACTFractalkine (FKN) is a chemokine with several roles, including chemotaxis; adhesion; and immune damage, which also participates in cell inflammation and apoptosis and responds to the pathogenesis of autoimmune diseases. Given the involvement of regulatory T cells (Treg) cells in autoimmune diseases, this study investigated the regulatory mechanism of FKN in renal injury and Treg apoptosis via the p38 mitogen-activated protein kinase (p38MAPK) signaling pathway in lupus-prone mice. Lupus was induced in BALB/c female mice by inj...
Source: Experimental Cell Research - June 14, 2021 Category: Cytology Authors: Jingxue Ma Qiming Gong Xiuhong Pan Pengwei Guo Linlin He Yanwu You Source Type: research

Necrosulfonamide reverses pyroptosis-induced inhibition of proliferation and differentiation of osteoblasts through the NLRP3/caspase-1/GSDMD pathway
Exp Cell Res. 2021 Jun 10:112648. doi: 10.1016/j.yexcr.2021.112648. Online ahead of print.ABSTRACTThe acute inflammatory stimulation occurring after a bone fracture regulates the repair and healing of local bone injury; however, under certain conditions, pyroptosis may occur in osteoblasts, which affects osteoblast proliferation and differentiation, thereby affecting the growth, development and morphological changes of bone tissue. The aim of the present study was to examine the effect of the pyroptosis inhibitor necrosulfonamide (NSA) on the proliferation and differentiation of osteoblasts and elucidate the underlying mec...
Source: Experimental Cell Research - June 13, 2021 Category: Cytology Authors: Jingliao Zhang Kuanhai Wei Source Type: research

Necrosulfonamide reverses pyroptosis-induced inhibition of proliferation and differentiation of osteoblasts through the NLRP3/caspase-1/GSDMD pathway
Exp Cell Res. 2021 Jun 10:112648. doi: 10.1016/j.yexcr.2021.112648. Online ahead of print.ABSTRACTThe acute inflammatory stimulation occurring after a bone fracture regulates the repair and healing of local bone injury; however, under certain conditions, pyroptosis may occur in osteoblasts, which affects osteoblast proliferation and differentiation, thereby affecting the growth, development and morphological changes of bone tissue. The aim of the present study was to examine the effect of the pyroptosis inhibitor necrosulfonamide (NSA) on the proliferation and differentiation of osteoblasts and elucidate the underlying mec...
Source: Experimental Cell Research - June 13, 2021 Category: Cytology Authors: Jingliao Zhang Kuanhai Wei Source Type: research

Tbx18-positive cells-derived myofibroblasts contribute to renal interstitial fibrosis via transforming growth factor- β signaling
Exp Cell Res. 2021 Jun 9:112682. doi: 10.1016/j.yexcr.2021.112682. Online ahead of print.ABSTRACTIt has been demonstrated that the T-box family transcription factor 18 (Tbx18) -positive cells give rise to renal mesenchymal cells and contribute to the development of the urinary system. However, it is unclear whether Tbx18-positive cells are the origin of the myofibroblasts during renal fibrosis. The present study aimed to determine the contribution of Tbx18-positive cells in kidney fibrosis and their underlying mechanism. We show that Tbx18-positivecells contribute to the development of the urinary system, especially renal ...
Source: Experimental Cell Research - June 12, 2021 Category: Cytology Authors: Xin Yuan Ling Zhang Jianlin Du Source Type: research

Dexamethasone reduces the formation of thoracic aortic aneurysm and dissection in a murine model
Exp Cell Res. 2021 Jun 10:112703. doi: 10.1016/j.yexcr.2021.112703. Online ahead of print.ABSTRACTThoracic aortic aneurysm and dissection (TAAD) is a life-threatening vascular disease with no effective pharmaceutical therapies currently available. Inflammation plays a key role in the progression of aneurysms. Dexamethasone (DEX), a synthetic glucocorticoid, has showed alleviating effects on cells in vitro from TAAD patients. Here we performed a study aiming at investigating the protective role of DEX in a β-aminopropionitrile monofumarate (BAPN)-induced TAAD mouse model. DEX (dose: 0.04 mg/kg/day) treatment significan...
Source: Experimental Cell Research - June 12, 2021 Category: Cytology Authors: Xipeng Wang Xiaoping Zhang Tao Qiu Yang Yang Qingle Li Xiaoming Zhang Source Type: research

Tbx18-positive cells-derived myofibroblasts contribute to renal interstitial fibrosis via transforming growth factor- β signaling
Exp Cell Res. 2021 Jun 9:112682. doi: 10.1016/j.yexcr.2021.112682. Online ahead of print.ABSTRACTIt has been demonstrated that the T-box family transcription factor 18 (Tbx18) -positive cells give rise to renal mesenchymal cells and contribute to the development of the urinary system. However, it is unclear whether Tbx18-positive cells are the origin of the myofibroblasts during renal fibrosis. The present study aimed to determine the contribution of Tbx18-positive cells in kidney fibrosis and their underlying mechanism. We show that Tbx18-positivecells contribute to the development of the urinary system, especially renal ...
Source: Experimental Cell Research - June 12, 2021 Category: Cytology Authors: Xin Yuan Ling Zhang Jianlin Du Source Type: research

Dexamethasone reduces the formation of thoracic aortic aneurysm and dissection in a murine model
Exp Cell Res. 2021 Jun 10:112703. doi: 10.1016/j.yexcr.2021.112703. Online ahead of print.ABSTRACTThoracic aortic aneurysm and dissection (TAAD) is a life-threatening vascular disease with no effective pharmaceutical therapies currently available. Inflammation plays a key role in the progression of aneurysms. Dexamethasone (DEX), a synthetic glucocorticoid, has showed alleviating effects on cells in vitro from TAAD patients. Here we performed a study aiming at investigating the protective role of DEX in a β-aminopropionitrile monofumarate (BAPN)-induced TAAD mouse model. DEX (dose: 0.04 mg/kg/day) treatment significan...
Source: Experimental Cell Research - June 12, 2021 Category: Cytology Authors: Xipeng Wang Xiaoping Zhang Tao Qiu Yang Yang Qingle Li Xiaoming Zhang Source Type: research

Expression of membrane protein disulphide isomerase A1 (PDIA1) disrupt a reducing microenvironment in endometrial epithelium for embryo implantation
Exp Cell Res. 2021 Jun 7:112665. doi: 10.1016/j.yexcr.2021.112665. Online ahead of print.ABSTRACTVarious proteins in the endometrial epithelium are differentially expressed in the receptive phase and play a pivotal role in embryo implantation. The Protein Disulphide Isomerase (PDI) family contains 21 members that function as chaperone proteins through their redox activities. Although total PDIA1 protein expression was high in four common receptive (Ishikawa and RL95-2) and non-receptive (HEC1-B and AN3CA) endometrial epithelial cell lines, significantly higher membrane PDIA1 expression was found in non-receptive AN3CA cell...
Source: Experimental Cell Research - June 10, 2021 Category: Cytology Authors: Sudini R Fernando Cheuk-Lun Lee Benancy Pc Wong Kiu-Wai Cheng Yin-Lau Lee Ernest Hy Ng William Sb Yeung Kai-Fai Lee Source Type: research

CD47 blockade enhances therapeutic efficacy of cisplatin against lung carcinoma in a murine model
Exp Cell Res. 2021 Jun 7:112677. doi: 10.1016/j.yexcr.2021.112677. Online ahead of print.ABSTRACTCisplatin (CDDP) is the first generation of platinum-based drug and is widely used to treat many cancers due to its potency. The present study aims to explore the effects of CDDP on lung carcinoma and its relationship with macrophage phagocytosis. In in vitro study, murine and human lung cancer cell lines were applied and treated with CDDP, CD47 antibody (aCD47), or CDDP plus aCD47. In in vivo study, a tumor xenograft animal model was treated with CDDP, aCD47, or CDDP plus aCD47. Real-time PCR was applied to determine the mRNA ...
Source: Experimental Cell Research - June 10, 2021 Category: Cytology Authors: Zhilei Cui Dengfei Xu Fafu Zhang Jinyuan Sun Lin Song Wenjing Ye Junxiang Zeng Min Zhou Zhengshang Ruan Linlin Zhang Rongrong Ren Source Type: research

Expression of membrane protein disulphide isomerase A1 (PDIA1) disrupt a reducing microenvironment in endometrial epithelium for embryo implantation
Exp Cell Res. 2021 Jun 7:112665. doi: 10.1016/j.yexcr.2021.112665. Online ahead of print.ABSTRACTVarious proteins in the endometrial epithelium are differentially expressed in the receptive phase and play a pivotal role in embryo implantation. The Protein Disulphide Isomerase (PDI) family contains 21 members that function as chaperone proteins through their redox activities. Although total PDIA1 protein expression was high in four common receptive (Ishikawa and RL95-2) and non-receptive (HEC1-B and AN3CA) endometrial epithelial cell lines, significantly higher membrane PDIA1 expression was found in non-receptive AN3CA cell...
Source: Experimental Cell Research - June 10, 2021 Category: Cytology Authors: Sudini R Fernando Cheuk-Lun Lee Benancy Pc Wong Kiu-Wai Cheng Yin-Lau Lee Ernest Hy Ng William Sb Yeung Kai-Fai Lee Source Type: research

CD47 blockade enhances therapeutic efficacy of cisplatin against lung carcinoma in a murine model
Exp Cell Res. 2021 Jun 7:112677. doi: 10.1016/j.yexcr.2021.112677. Online ahead of print.ABSTRACTCisplatin (CDDP) is the first generation of platinum-based drug and is widely used to treat many cancers due to its potency. The present study aims to explore the effects of CDDP on lung carcinoma and its relationship with macrophage phagocytosis. In in vitro study, murine and human lung cancer cell lines were applied and treated with CDDP, CD47 antibody (aCD47), or CDDP plus aCD47. In in vivo study, a tumor xenograft animal model was treated with CDDP, aCD47, or CDDP plus aCD47. Real-time PCR was applied to determine the mRNA ...
Source: Experimental Cell Research - June 10, 2021 Category: Cytology Authors: Zhilei Cui Dengfei Xu Fafu Zhang Jinyuan Sun Lin Song Wenjing Ye Junxiang Zeng Min Zhou Zhengshang Ruan Linlin Zhang Rongrong Ren Source Type: research

Application of iPSCs Derived Pancreatic β-Like Cells Using Pancreatic Bio-Scaffold
This study aimed toengineer a pancreatic tissue. Intact rat pancreases were successfully decellularized, and were reseeded with human-induced pluripotent stem cells using different 2D and 3D culture growth factors. The differentiation process was assessed for the presence of a pancreas-like tissue. The histology and SEM analysis revealed cell attachment in all samples, except for the Exp4, and the Flow-cytometry provided 87% viability for the differentiated cells. In Exp1, PDX1 with the positive expression of 2.87±0.06 was dramatically higher than Exp2 with a 2.44±0.06 reaction. NGN3-reactions were 8±0...
Source: Experimental Cell Research - June 9, 2021 Category: Cytology Authors: Javad Hashemi Abdol-Mohammad Kajbafzadeh Fariba Ghorbani Masoud Soleimani Ehsan Arefian Reza Khorramirouz Seyed Ehsan Enderami Parvin Pasalar Source Type: research

Lipid accumulation-induced hepatocyte senescence regulates the activation of hepatic stellate cells through the Nrf2-antioxidant response element pathway
In conclusion, this study confirmed that lipid accumulation induces hepatocyte senescence, which leads to HSC activation and development of hepatic fibrosis. Increasing the activity of the Nrf2-ARE antioxidant pathway in senescent hepatocytes elicited the opposite effect, suggesting that targeting Nrf2 may prevent or delay the progression of aging-related liver fibrosis in NASH.PMID:34107274 | DOI:10.1016/j.yexcr.2021.112689 (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 9, 2021 Category: Cytology Authors: Huiyuan Yu Xin Jiang Fangyuan Dong Fan Zhang Xueying Ji Mengjuan Xue Fan Yang Jie Chen Xiaona Hu Zhijun Bao Source Type: research

Application of iPSCs Derived Pancreatic β-Like Cells Using Pancreatic Bio-Scaffold
This study aimed toengineer a pancreatic tissue. Intact rat pancreases were successfully decellularized, and were reseeded with human-induced pluripotent stem cells using different 2D and 3D culture growth factors. The differentiation process was assessed for the presence of a pancreas-like tissue. The histology and SEM analysis revealed cell attachment in all samples, except for the Exp4, and the Flow-cytometry provided 87% viability for the differentiated cells. In Exp1, PDX1 with the positive expression of 2.87±0.06 was dramatically higher than Exp2 with a 2.44±0.06 reaction. NGN3-reactions were 8±0...
Source: Experimental Cell Research - June 9, 2021 Category: Cytology Authors: Javad Hashemi Abdol-Mohammad Kajbafzadeh Fariba Ghorbani Masoud Soleimani Ehsan Arefian Reza Khorramirouz Seyed Ehsan Enderami Parvin Pasalar Source Type: research

Lipid accumulation-induced hepatocyte senescence regulates the activation of hepatic stellate cells through the Nrf2-antioxidant response element pathway
In conclusion, this study confirmed that lipid accumulation induces hepatocyte senescence, which leads to HSC activation and development of hepatic fibrosis. Increasing the activity of the Nrf2-ARE antioxidant pathway in senescent hepatocytes elicited the opposite effect, suggesting that targeting Nrf2 may prevent or delay the progression of aging-related liver fibrosis in NASH.PMID:34107274 | DOI:10.1016/j.yexcr.2021.112689 (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 9, 2021 Category: Cytology Authors: Huiyuan Yu Xin Jiang Fangyuan Dong Fan Zhang Xueying Ji Mengjuan Xue Fan Yang Jie Chen Xiaona Hu Zhijun Bao Source Type: research

Cell Stress during Development and Disease - Special Issue, Review article Balancing DNA repair to prevent ageing and cancer
Exp Cell Res. 2021 Jun 5:112679. doi: 10.1016/j.yexcr.2021.112679. Online ahead of print.ABSTRACTDNA damage is a constant stressor to the cell. Persistent damage to the DNA over time results in an increased risk of mutation and an accumulation of mutations with age. Loss of efficient DNA damage repair can lead to accelerated ageing phenotypes or an increased cancer risk, and the trade-off between cancer susceptibility and longevity is often driven by the cell's response to DNA damage. High levels of mutations in DNA repair mutants often leads to excessive cell death and stem cell exhaustion which may promote premature agei...
Source: Experimental Cell Research - June 8, 2021 Category: Cytology Authors: Eleanor Rachel Stead Ivana Bjedov Source Type: research

Downregulation of miR-892b inhibits the progression of osteoarthritis via targeting Cyclin D1 and Cyclin D2
CONCLUSION: MiR-892b antagomir inhibits the progression of OA via targeting Cyclin D1 and Cyclin D2. Thus, our finding might supply a novel target for OA treatment.PMID:34102226 | DOI:10.1016/j.yexcr.2021.112683 (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 8, 2021 Category: Cytology Authors: Jiajun Zhao Zhen Zhang Tantan Zuo Jie Yu Shihao Yang Yitao Yang Xinqi Li Jia Zheng Source Type: research

Endothelial surface translocation of mitochondrial PDCE2 involves the non-canonical secretory autophagy pathway: putative molecular target for radiation-guided drug delivery
In this study we examined the mechanism of PDCE2 trafficking in human endothelial cells to better understand its suitability as a vascular target. Ionizing radiation induced PDCE2 surface localization in association with accumulation of autophagosome markers (L3CB and p62) indicative of late-stage inhibition of autophagic flux. This effect was abolished in the presence of Rapamycin, an autophagy-inducer, but replicated in the presence of Bafilomycin A, an autophagy blocker. PDCE2 co-localized with lysosomal markers of the canonical degradative autophagy pathway in response to radiation but also with recycling endosomes and...
Source: Experimental Cell Research - June 7, 2021 Category: Cytology Authors: F Faqihi M A Stoodley L S McRobb Source Type: research

ZEB1 induces non-small cell lung cancer development by targeting microRNA-320a to increase the expression of RAD51AP1
In this study, we investigated the interactions and expressions of zinc finger E-box binding homeobox 1 (ZEB1), microRNA-320a (miR-320a) and RAD51-associated protein 1 (RAD51AP1) in NSCLC tissues to determine the roles of ZEB1 in regulation of miR-320a and RAD51AP1 in the development and metastasis of NSCLC. First, the expression levels of miR-320a and ZEB1 were quantified in NSCLC tissues and cells. Transfection assay was conducted to identify the effects of miR-320a on the progression of NSCLC cells. The interaction of miR-320a with ZEB1 and RAD51AP1 was predicted and verified using dual-luciferase reporter gene assay an...
Source: Experimental Cell Research - June 7, 2021 Category: Cytology Authors: Huifeng Wang Hui Dong Lijiao Qiao Yuanyuan Wu Bo Wu Xiangming Jin Source Type: research

Endothelial surface translocation of mitochondrial PDCE2 involves the non-canonical secretory autophagy pathway: putative molecular target for radiation-guided drug delivery
In this study we examined the mechanism of PDCE2 trafficking in human endothelial cells to better understand its suitability as a vascular target. Ionizing radiation induced PDCE2 surface localization in association with accumulation of autophagosome markers (L3CB and p62) indicative of late-stage inhibition of autophagic flux. This effect was abolished in the presence of Rapamycin, an autophagy-inducer, but replicated in the presence of Bafilomycin A, an autophagy blocker. PDCE2 co-localized with lysosomal markers of the canonical degradative autophagy pathway in response to radiation but also with recycling endosomes and...
Source: Experimental Cell Research - June 7, 2021 Category: Cytology Authors: F Faqihi M A Stoodley L S McRobb Source Type: research

ZEB1 induces non-small cell lung cancer development by targeting microRNA-320a to increase the expression of RAD51AP1
In this study, we investigated the interactions and expressions of zinc finger E-box binding homeobox 1 (ZEB1), microRNA-320a (miR-320a) and RAD51-associated protein 1 (RAD51AP1) in NSCLC tissues to determine the roles of ZEB1 in regulation of miR-320a and RAD51AP1 in the development and metastasis of NSCLC. First, the expression levels of miR-320a and ZEB1 were quantified in NSCLC tissues and cells. Transfection assay was conducted to identify the effects of miR-320a on the progression of NSCLC cells. The interaction of miR-320a with ZEB1 and RAD51AP1 was predicted and verified using dual-luciferase reporter gene assay an...
Source: Experimental Cell Research - June 7, 2021 Category: Cytology Authors: Huifeng Wang Hui Dong Lijiao Qiao Yuanyuan Wu Bo Wu Xiangming Jin Source Type: research

Ferredoxin reductase regulates proliferation, differentiation, cell cycle and lipogenesis but not apoptosis in SZ95 sebocytes
Exp Cell Res. 2021 Jun 3:112680. doi: 10.1016/j.yexcr.2021.112680. Online ahead of print.ABSTRACTFerredoxin reductase (FDXR), a mitochondrial membrane-associated flavoprotein, is essential for electron transfer and modulates p53-dependent apoptosis in cancer cells.FDXR may be implicated in epidermal and sebocytic differentiation, but its explicit function in sebocytes remains to be elucidated. In the present study, immunohistochemistry revealed that FDXR expression was increased in sebaceous cells of acne lesions. FDXR, PPARγ, LXRα/β, SREBP1 and Sox9 expression was incremental during sebocyte differentiati...
Source: Experimental Cell Research - June 6, 2021 Category: Cytology Authors: Meng-Tong Li Xiao-Xue Pi Xiao-Lin Cai Si Liu Zheng Huang Christos C Zouboulis Yi-Ming Fan Ge Shi Source Type: research

Development of a recombinant anti-VEGFR2-EPCAM bispecific antibody to improve antiangiogenic efficiency
Exp Cell Res. 2021 Jun 3:112685. doi: 10.1016/j.yexcr.2021.112685. Online ahead of print.ABSTRACTTumor progression and metastasis, especially in invasive cancers (such as triple-negative breast cancer [TNBC]), depend on angiogenesis, in which vascular epithelial growth factor (VEGF)/vascular epithelial growth factor receptor [1] has a decisive role, followed by the metastatic spread of cancer cells. Although some studies have shown that anti-VEGFR2/VEGF monoclonal antibodies demonstrated favorable results in the clinic, this approach is not efficient, and further investigations are needed to improve the quality of cancer t...
Source: Experimental Cell Research - June 6, 2021 Category: Cytology Authors: Khadijeh Barzaman Mitra Samadi Shima Moradi-Kalbolandi Keivan Majidzadeh-A Malihe Salehi Neda Jalili Mir Hadi Jazayeri Samaneh Khorammi Behrad Darvishi Vahid Siavashi Mahdi Shekarabi Leila Farahmand Source Type: research

Ferredoxin reductase regulates proliferation, differentiation, cell cycle and lipogenesis but not apoptosis in SZ95 sebocytes
Exp Cell Res. 2021 Jun 3:112680. doi: 10.1016/j.yexcr.2021.112680. Online ahead of print.ABSTRACTFerredoxin reductase (FDXR), a mitochondrial membrane-associated flavoprotein, is essential for electron transfer and modulates p53-dependent apoptosis in cancer cells.FDXR may be implicated in epidermal and sebocytic differentiation, but its explicit function in sebocytes remains to be elucidated. In the present study, immunohistochemistry revealed that FDXR expression was increased in sebaceous cells of acne lesions. FDXR, PPARγ, LXRα/β, SREBP1 and Sox9 expression was incremental during sebocyte differentiati...
Source: Experimental Cell Research - June 6, 2021 Category: Cytology Authors: Meng-Tong Li Xiao-Xue Pi Xiao-Lin Cai Si Liu Zheng Huang Christos C Zouboulis Yi-Ming Fan Ge Shi Source Type: research

Development of a recombinant anti-VEGFR2-EPCAM bispecific antibody to improve antiangiogenic efficiency
Exp Cell Res. 2021 Jun 3:112685. doi: 10.1016/j.yexcr.2021.112685. Online ahead of print.ABSTRACTTumor progression and metastasis, especially in invasive cancers (such as triple-negative breast cancer [TNBC]), depend on angiogenesis, in which vascular epithelial growth factor (VEGF)/vascular epithelial growth factor receptor [1] has a decisive role, followed by the metastatic spread of cancer cells. Although some studies have shown that anti-VEGFR2/VEGF monoclonal antibodies demonstrated favorable results in the clinic, this approach is not efficient, and further investigations are needed to improve the quality of cancer t...
Source: Experimental Cell Research - June 6, 2021 Category: Cytology Authors: Khadijeh Barzaman Mitra Samadi Shima Moradi-Kalbolandi Keivan Majidzadeh-A Malihe Salehi Neda Jalili Mir Hadi Jazayeri Samaneh Khorammi Behrad Darvishi Vahid Siavashi Mahdi Shekarabi Leila Farahmand Source Type: research

Sorafenib derivatives-functionalized gold nanoparticles confer protection against tumor angiogenesis and proliferation via suppression of EGFR and VEGFR-2
Exp Cell Res. 2021 Jun 2:112633. doi: 10.1016/j.yexcr.2021.112633. Online ahead of print.ABSTRACTIncreasing evidence has reported the great promise of multicomponent and multifunctional nanoparticles as advanced therapies of human diseases, especially tumors. In the current study, we investigated the functionality of new sorafenib derivatives-capped gold nanoparticles (AuNPs-New Sor) in tumor growth as well as their anti-tumor mechanisms. Initially, new sorafenib derivatives were constructed and combined with AuNPs to form AuNPs-New Sor. the properties of synthesized AuNPs-New Sor were identified in a mouse model of tumori...
Source: Experimental Cell Research - June 5, 2021 Category: Cytology Authors: Wei Huang Yitao Xing Lile Zhu Jinsheng Zhuo Min Cai Source Type: research

Sorafenib derivatives-functionalized gold nanoparticles confer protection against tumor angiogenesis and proliferation via suppression of EGFR and VEGFR-2
Exp Cell Res. 2021 Jun 2:112633. doi: 10.1016/j.yexcr.2021.112633. Online ahead of print.ABSTRACTIncreasing evidence has reported the great promise of multicomponent and multifunctional nanoparticles as advanced therapies of human diseases, especially tumors. In the current study, we investigated the functionality of new sorafenib derivatives-capped gold nanoparticles (AuNPs-New Sor) in tumor growth as well as their anti-tumor mechanisms. Initially, new sorafenib derivatives were constructed and combined with AuNPs to form AuNPs-New Sor. the properties of synthesized AuNPs-New Sor were identified in a mouse model of tumori...
Source: Experimental Cell Research - June 5, 2021 Category: Cytology Authors: Wei Huang Yitao Xing Lile Zhu Jinsheng Zhuo Min Cai Source Type: research

Glucocorticoids activate Yes-associated protein in human vocal fold fibroblasts
Exp Cell Res. 2021 Jun 1:112681. doi: 10.1016/j.yexcr.2021.112681. Online ahead of print.ABSTRACTFibrosis of the vocal folds poses a substantive clinical challenge potentially underlying the rapid proliferation of direct steroid injections into the upper airway. The variable clinical response to glucocorticoids (GCs) in the vocal folds is likely related to diversity inherent to GCs and both patient-specific, and upstream, cell-specific responses to GCs. Broadly, we hypothesize the disparity in clinical outcomes are due to undesirable effects of GCs on resident fibroblasts. Transcriptome analysis identified significant GC-m...
Source: Experimental Cell Research - June 4, 2021 Category: Cytology Authors: Ryosuke Nakamura Renjie Bing Carina P Doyle Michael J Garabedian Ryan C Branski Source Type: research

Checkpoint kinases are required for oocyte meiotic progression by the maintenance of normal spindle structure and chromosome condensation
In conclusion, our results further understand the functions and regulatory mechanism of Chk1/2 during oocyte meiotic maturation.PMID:34081985 | DOI:10.1016/j.yexcr.2021.112657 (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 3, 2021 Category: Cytology Authors: Xiao-Ming Liu Fang Chen Li Wang Fan Zhang Li-Jun Huo Source Type: research

Checkpoint kinases are required for oocyte meiotic progression by the maintenance of normal spindle structure and chromosome condensation
In conclusion, our results further understand the functions and regulatory mechanism of Chk1/2 during oocyte meiotic maturation.PMID:34081985 | DOI:10.1016/j.yexcr.2021.112657 (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 3, 2021 Category: Cytology Authors: Xiao-Ming Liu Fang Chen Li Wang Fan Zhang Li-Jun Huo Source Type: research

Vimentin loss promotes cancer proliferation through up-regulating Rictor/AKT/ β-catenin signaling pathway
In this study, we investigated the participation of vimentin in regulating cancer proliferation by silencing VIM gene in four cancer cell lines. Our results demonstrated that vimentin loss significantly induced cancer cell proliferation both in vitro and in vivo, which has not been reported so far. Mechanistically, knockdown of vimentin expression activated AKT phosphorylation and its downstream β-catenin signaling. Nuclear translocation and transcriptional activity of β-catenin was enhanced after silencing vimentin expression. Furthermore, vimentin loss could prevent Rictor from autophagy-dependent degradation v...
Source: Experimental Cell Research - May 30, 2021 Category: Cytology Authors: Youxiang Ding Conggai Lv You Zhou Heng Zhang Li Zhao Yuting Xu Xiangshan Fan Source Type: research

Vimentin loss promotes cancer proliferation through up-regulating Rictor/AKT/ β-catenin signaling pathway
In this study, we investigated the participation of vimentin in regulating cancer proliferation by silencing VIM gene in four cancer cell lines. Our results demonstrated that vimentin loss significantly induced cancer cell proliferation both in vitro and in vivo, which has not been reported so far. Mechanistically, knockdown of vimentin expression activated AKT phosphorylation and its downstream β-catenin signaling. Nuclear translocation and transcriptional activity of β-catenin was enhanced after silencing vimentin expression. Furthermore, vimentin loss could prevent Rictor from autophagy-dependent degradation v...
Source: Experimental Cell Research - May 30, 2021 Category: Cytology Authors: Youxiang Ding Conggai Lv You Zhou Heng Zhang Li Zhao Yuting Xu Xiangshan Fan Source Type: research

Vimentin loss promotes cancer proliferation through up-regulating Rictor/AKT/ β-catenin signaling pathway
In this study, we investigated the participation of vimentin in regulating cancer proliferation by silencing VIM gene in four cancer cell lines. Our results demonstrated that vimentin loss significantly induced cancer cell proliferation both in vitro and in vivo, which has not been reported so far. Mechanistically, knockdown of vimentin expression activated AKT phosphorylation and its downstream β-catenin signaling. Nuclear translocation and transcriptional activity of β-catenin was enhanced after silencing vimentin expression. Furthermore, vimentin loss could prevent Rictor from autophagy-dependent degradation v...
Source: Experimental Cell Research - May 30, 2021 Category: Cytology Authors: Youxiang Ding Conggai Lv You Zhou Heng Zhang Li Zhao Yuting Xu Xiangshan Fan Source Type: research

Vimentin loss promotes cancer proliferation through up-regulating Rictor/AKT/ β-catenin signaling pathway
In this study, we investigated the participation of vimentin in regulating cancer proliferation by silencing VIM gene in four cancer cell lines. Our results demonstrated that vimentin loss significantly induced cancer cell proliferation both in vitro and in vivo, which has not been reported so far. Mechanistically, knockdown of vimentin expression activated AKT phosphorylation and its downstream β-catenin signaling. Nuclear translocation and transcriptional activity of β-catenin was enhanced after silencing vimentin expression. Furthermore, vimentin loss could prevent Rictor from autophagy-dependent degradation v...
Source: Experimental Cell Research - May 30, 2021 Category: Cytology Authors: Youxiang Ding Conggai Lv You Zhou Heng Zhang Li Zhao Yuting Xu Xiangshan Fan Source Type: research

Exosomes derived from hypoxic bone marrow mesenchymal stem cells rescue OGD-induced injury in neural cells by suppressing NLRP3 inflammasome-mediated pyroptosis
In this study, we investigated the neuroprotective effects of normoxic and hypoxic bone marrow mesenchymal stromal cells-derived exosomes (N-BM-MSCs-Exo and H-BM-MSCs-Exo, respectively) on oxygen-glucose deprivation (OGD) injury in mouse neuroblastoma N2a cells and rat primary cortical neurons. The proportions of dead cells in N2a and primary cortical neurons after OGD injury were significantly increased, and N-BM-MSCs-Exo (40 μg/ml) could reduce the ratios, noteworthily, the protective effects of H-BM-MSCs-Exo (40 μg/ml) were more potent. Western blotting analysis indicated that N-BM-MSCs-Exo decreased the expressio...
Source: Experimental Cell Research - May 29, 2021 Category: Cytology Authors: Xiuwen Kang Lei Jiang Xufeng Chen Xi Wang Shuangshuang Gu Jun Wang Yuanhui Zhu Xuexue Xie Hang Xiao Jinsong Zhang Source Type: research

HEPATIC stellate cell-derived exosomes MODULATE macrophage inflammatory response
CONCLUSIONS: HSC-EXOs associate with macrophages and stimulate cytokine synthesis-release and macrophage migration. Constructing a comprehensive understanding of EXOs interactions between liver cell populations in the setting of inflammation/fibrosis increases the potential for developing new diagnostic/therapeutic approaches.PMID:34051242 | DOI:10.1016/j.yexcr.2021.112663 (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 29, 2021 Category: Cytology Authors: Jennifer H Benbow Emilio Marrero Rachel M McGee Elizabeth Brandon-Warner Neha Attal Nicole A Feilen Catherine R Culberson Iain H McKillop Laura W Schrum Source Type: research

Exosomes derived from hypoxic bone marrow mesenchymal stem cells rescue OGD-induced injury in neural cells by suppressing NLRP3 inflammasome-mediated pyroptosis
In this study, we investigated the neuroprotective effects of normoxic and hypoxic bone marrow mesenchymal stromal cells-derived exosomes (N-BM-MSCs-Exo and H-BM-MSCs-Exo, respectively) on oxygen-glucose deprivation (OGD) injury in mouse neuroblastoma N2a cells and rat primary cortical neurons. The proportions of dead cells in N2a and primary cortical neurons after OGD injury were significantly increased, and N-BM-MSCs-Exo (40 μg/ml) could reduce the ratios, noteworthily, the protective effects of H-BM-MSCs-Exo (40 μg/ml) were more potent. Western blotting analysis indicated that N-BM-MSCs-Exo decreased the expressio...
Source: Experimental Cell Research - May 29, 2021 Category: Cytology Authors: Xiuwen Kang Lei Jiang Xufeng Chen Xi Wang Shuangshuang Gu Jun Wang Yuanhui Zhu Xuexue Xie Hang Xiao Jinsong Zhang Source Type: research

HEPATIC stellate cell-derived exosomes MODULATE macrophage inflammatory response
CONCLUSIONS: HSC-EXOs associate with macrophages and stimulate cytokine synthesis-release and macrophage migration. Constructing a comprehensive understanding of EXOs interactions between liver cell populations in the setting of inflammation/fibrosis increases the potential for developing new diagnostic/therapeutic approaches.PMID:34051242 | DOI:10.1016/j.yexcr.2021.112663 (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 29, 2021 Category: Cytology Authors: Jennifer H Benbow Emilio Marrero Rachel M McGee Elizabeth Brandon-Warner Neha Attal Nicole A Feilen Catherine R Culberson Iain H McKillop Laura W Schrum Source Type: research

In vitro assessment of a synergistic combination of Gemcitabine and Zebularine in pancreatic cancer cells
In this study, we investigated the effect of a CDA inhibitor - Zebularine (Zeb) on anticancer activity of Gem in pancreatic cancer cell lines MiaPaCa-2, BxPC-3, and Panc-1. Zeb treatment synergistically increased Gem-induced cytotoxicity in all three pancreatic cancer cell lines. The strongest synergistic activity was found at 1:10 molar ratio of Gem/Zeb (combination index 0.04-0.4). Additionally, Gem+Zeb treated cells showed marked decreased in the expressions of anti-apoptotic protein including Bcl-2 and survivin while significantly increased the cleaved caspase-3, and loss of mitochondrial membrane potential was observe...
Source: Experimental Cell Research - May 28, 2021 Category: Cytology Authors: Manali Patki Aishwarya Saraswat Shraddha Bhutkar Vikas Dukhande Ketankumar Patel Source Type: research

RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m < sup > 5 < /sup > C-dependent manner
Exp Cell Res. 2021 May 25:112664. doi: 10.1016/j.yexcr.2021.112664. Online ahead of print.ABSTRACTRNA methyltransferase NSUN2 is involved in cell proliferation and invasion in a variety of tumors. However, the expression, function, and mechanism of NSUN2 in hypopharyngeal squamous cell carcinoma (HPSCC) remains unknown. We used a bioinformatics database, polymerase chain reaction, cell culture and transfection, immunohistochemistry, cell proliferation assay, wound healing experiments, transwell assays, western blotting, RNA-seq detection, dual-luciferase reporter assay, in vivo experiments, and a dot blot assay to evaluate...
Source: Experimental Cell Research - May 28, 2021 Category: Cytology Authors: Lixiao Chen Jian Ding Baoxin Wang Xinwei Chen Xinjiang Ying Ziwei Yu Pin Dong Source Type: research

Phospholipase D and phosphatidylinositol-4-phosphate 5-kinase 1 are involved in the regulation of oligodendrocyte morphological differentiation
Exp Cell Res. 2021 May 24:112654. doi: 10.1016/j.yexcr.2021.112654. Online ahead of print.ABSTRACTOligodendroglial cells (oligodendrocytes) differentiate to form the myelin that wraps neuronal axons in the central nervous system (CNS). This myelin sheath supports the propagation of saltatory conduction and protects axons from physical stresses. When oligodendrocytes do not normally differentiate to myelinate axons, their key functions as oligodendrocytes in the CNS are severely impaired. The molecular mechanics that control differentiation still remain to be clarified. Arf6 belongs to the small GTPase family and is known t...
Source: Experimental Cell Research - May 27, 2021 Category: Cytology Authors: Yukino Kato Arisa Ochiai Yoichi Seki Takako Morimoto Hiroaki Oizumi Katsuya Ohbuchi Kazushige Mizoguchi Masahiro Yamamoto Hiroyuki Sakagami Yuki Miyamoto Junji Yamauchi Source Type: research

TWIST2 inhibits EMT and induces oxidative stress in lung cancer cells by regulating the FGF21-mediated AMPK/mTOR pathway
Exp Cell Res. 2021 May 24:112661. doi: 10.1016/j.yexcr.2021.112661. Online ahead of print.ABSTRACTTwist related protein 2 (TWIST2) plays an important role in bone development, tumorigenesis, tumour progression and epithelial mesenchymal transition (EMT). At present, there are few reports about the role of TWIST2 in lung cancer, which need to be further explored. Therefore, the purpose of this study is to explore the role and molecular mechanism of TWIST2 in the occurrence and development of lung cancer. The expression of TWIST2 in tissues of patients and cell lines was measured using RT-qPCR and western blotting. MTT and C...
Source: Experimental Cell Research - May 27, 2021 Category: Cytology Authors: Yingjian Song Wei Zhang Jiuxu Zhang Zhaolei You Tao Hu Guangyuan Shao Zheng Zhang Zhicheng Xu Xiaofeng Yu Source Type: research