Cytoskeletal Tubulin competes with actin to increase deformability of metastatic melanoma cells.
Abstract The formation of membrane protrusions during migration is reliant upon the cells' cytoskeletal structure and stiffness. It has been reported that actin disruption blocks protrusions and decreases cell stiffness whereas microtubule disruption blocks protrusion but increases stiffness in several cell types. In melanoma, cell migration is of concern as this cancer spreads unusually rapidly during early tumour development. The aim of this study was to characterise motility, structural properties and stiffness of human melanoma cells at radial growth phase (RGP), vertical growth phase (VGP), and metastatic sta...
Source: Experimental Cell Research - June 26, 2020 Category: Cytology Authors: Higgins G, Peres J, Abdalrahman T, Zaman MH, Lang DM, Prince S, Franz T Tags: Exp Cell Res Source Type: research

Lung-resident mesenchymal stem cells regulated the inflammatory responses in innate and adaptive immune cells through HVEM-BTLA pathway during ARDS.
Abstract Acute respiratory distress syndrome (ARDS) is an organ failure syndrome caused by overactivation of the immune system. Mesenchymal stem cells (MSCs) have been found to be effective in ARDS therapy due to their excellent immunomodulatory abilities; however, people are concerned about the safety of infusing exogenous cells. We found that rat lung-resident mesenchymal stem cells (LRMSCs) (Sca-1+CD45-CD31-) played important roles in regulating inflammation in the lungs during the pathogenesis of ARDS. LRMSCs could regulate the production of cytokines (TNF-α, MCP-1, and IL-10) by both innate and adaptive...
Source: Experimental Cell Research - June 26, 2020 Category: Cytology Authors: Cheng T, Feng Y, Chen X, Zhou J, Song Y Tags: Exp Cell Res Source Type: research

Microenvironment remodeled by tumor and stromal cells elevates fibroblast-derived COL1A1 and facilitates ovarian cancer metastasis.
Abstract Wide peritoneal metastasis is the cause of the highest lethality of ovarian cancer in gynecologic malignancies. Ascites play a key role in ovarian cancer metastasis, but involved mechanism is uncertain. Here, we performed a quantitative proteomics of ascites, and found that collagen type I alpha 1 (COL1A1) was notably elevated in ascites from epithelial ovarian cancer patients compared to normal peritoneal fluids, and verified that elevated COL1A1 was mainly originated from fibroblasts. COL1A1 promoted migration and invasion of ovarian cancer cells, but such effects were partially eliminated by COL1A1 ant...
Source: Experimental Cell Research - June 23, 2020 Category: Cytology Authors: Li M, Wang J, Wang C, Xia L, Xu J, Xie X, Lu W Tags: Exp Cell Res Source Type: research

Maternal exposure to Di-(2-ethylhexyl) phthalate (DEHP) activates the PI3K/Akt/mTOR signaling pathway in F1 and F2 generation adult mouse testis.
In this study, a F0 gestation DEHP exposure and transgenerational inheritance testis injury model was established to study the testis injury phenotype and the expression and activation of members of PI3K/Akt/mTOR signaling pathway in the testis of F1-F3 generation mice. We found that the bodyweight and the anogenital distance (AGD) are reduced only in F1 mice, the sperm motility and deformity decreased in F1-F3 mice, and the testicular histomorphology damagedin F1-F3 mice; however the sperm motility and deformity rates are increased and the histomorphological injury is repaired during the transgenerational process. We also...
Source: Experimental Cell Research - June 23, 2020 Category: Cytology Authors: Zhang J, Yao Y, Pan J, Guo X, Han X, Zhou J, Meng X Tags: Exp Cell Res Source Type: research

Camptothecin induced DDX5 degradation increased the camptothecin resistance of osteosarcoma.
Abstract Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. Unfortunately, chemo-resistance is a huge obstacle in the treatment of OS. However, the underlying molecular mechanisms of OS chemo-resistance still remain unknown. Here we reported that the resistance to camptothecin (cpt) therapy was driven by degradation of DDX5. DDX5 knockdown decreased cell death and DNA damage and recovered cell proliferation in cpt treated 143B cells. Furthermore, we found that DDX5 bound to NONO, a kind of DNA repairing protein, and regulated NONO functions. Our data verified that cpt-in...
Source: Experimental Cell Research - June 22, 2020 Category: Cytology Authors: Zhao X, Bao M, Zhang F, Wang W Tags: Exp Cell Res Source Type: research

Age-related taste cell generation in circumvallate papillae organoids via regulation of multiple signaling pathways.
Abstract Sense of taste is central to evaluate food before digestion. Taste stem cells undergo constant differentiation throughout the life. However, the mechanism underlying the generation of taste receptor cells is still not clear. Here, we cultured taste organoids from either Lgr5+ or Lgr5-cells, and found the preferential generation of Car4+ and Gustducin + taste receptor cells in organoids derived from Lgr5+ cells in circumvallate, foliate or fungiform papillae. Taste organoids derived from Lgr5+ cells in circumvallate papillae of neonatal mice showed stronger capacity to generate taste receptor cel...
Source: Experimental Cell Research - June 22, 2020 Category: Cytology Authors: Ren W, Liu Q, Zhang X, Yu Y Tags: Exp Cell Res Source Type: research

PRMT2 accelerates tumorigenesis of hepatocellular carcinoma by activating Bcl2 via histone H3R8 methylation.
Abstract Protein arginine methyltransferases (PRMTs) have been implicated in the development of various cancers. PRMT2, a member of the type I PRMT family, is overexpressed in multiple tumors. However, the expression and role of PRMT2 in hepatocellular carcinoma (HCC) have not been studied. Here, we discovered that PRMT2 expression is elevated in HCC tissues compared to the corresponding nontumor tissues, and PRMT2 overexpression is an independent predictor of poor prognosis in HCC patients. Depletion of PRMT2 in HCC cell lines inhibited their cell growth and induced apoptosis. Mechanistic investigations showed th...
Source: Experimental Cell Research - June 20, 2020 Category: Cytology Authors: Hu G, Yan C, Xie P, Cao Y, Shao J, Ge J Tags: Exp Cell Res Source Type: research

A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
Abstract Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically. Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties. Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanic...
Source: Experimental Cell Research - June 17, 2020 Category: Cytology Authors: Deng Z, Chen J, Lin B, Li J, Wang H, Wang D, Pang L, Zeng X, Wang H, Zhang Y Tags: Exp Cell Res Source Type: research

Identification of immune and non-immune cells in regenerating axolotl limbs by single-cell sequencing.
Abstract Immune cells are known to be critical for successful limb regeneration in the axolotl (Ambystoma mexicanum), but many details regarding their identity, behavior, and function are yet to be resolved. We isolated peripheral leukocytes from the blood of adult axolotls and then created two samples for single-cell sequencing: 1) peripheral leukocytes (N = 7889) and 2) peripheral leukocytes with presumptive macrophages from the intraperitoneal cavity (N = 4998). Using k-means clustering, we identified 6 cell populations from each sample that presented gene expression patterns indicative of erythrocyte...
Source: Experimental Cell Research - June 17, 2020 Category: Cytology Authors: Rodgers AK, Smith JJ, Voss SR Tags: Exp Cell Res Source Type: research

Insulin exacerbated high glucose-induced epithelial-mesenchymal transition in prostatic epithelial cells BPH-1 and prostate cancer cells PC-3 via MEK/ERK signaling pathway.
Abstract As two most common progressive diseases of aging, type 2 diabetes mellitus (T2DM) and benign prostatic hyperplasia (BPH) were all characterized by endocrine and metabolic disorders. Here, our clinical study showed that there were significant differences in fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR) and prostate volume (PV) between simple BPH patients and BPH complicated with T2DM patients. Further analysis showed that HOMA-IR was positively correlated with PV in BPH complicated with T2DM patients. The in vitro experiment results showed that high glucose (HG) pr...
Source: Experimental Cell Research - June 16, 2020 Category: Cytology Authors: Yang T, Zhou Y, Wang H, Chen S, Shen M, Hu Y, Wang T, Liu J, Jiang Z, Wang Z, Zhu X, Qian S, Yin X, Lu Q Tags: Exp Cell Res Source Type: research

Extracellular vesicles derived from macrophage promote angiogenesis In vitro and accelerate new vasculature formation In vivo.
CONCLUSION: Our results suggest that MAC-EVs have the potential to induce angiogenesis in vitro and in vivo, could serve as a pro-angiogenic alternative for ischemic diseases. PMID: 32561287 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 16, 2020 Category: Cytology Authors: Gangadaran P, Rajendran RL, Oh JM, Hong CM, Jeong SY, Lee SW, Lee J, Ahn BC Tags: Exp Cell Res Source Type: research

Extracellular vesicles carrying miR-193a derived from mesenchymal stem cells impede cell proliferation, migration and invasion of colon cancer by downregulating FAK.
In this study, we sought to investigate biological effects of miR-193a on colon cancer and its underlying mechanism. Microarray analysis was conducted to obtain the differentially expressed miRs and their target genes in colon cancer. Bone-marrow derived mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) were obtained. The functional roles of miR-193a and FAK in colon cancer were determined using loss- and gain-function experiments. The cell proliferation, and migration and invasion were evaluated by CCK-8 and Transwell assay respectively. Dual-luciferase reporter assay was performed to confirm the targeting re...
Source: Experimental Cell Research - June 12, 2020 Category: Cytology Authors: Ying H, Lin F, Ding R, Wang W, Hong W Tags: Exp Cell Res Source Type: research

Impact of T-cell receptor and B-cell receptor repertoire on the recurrence of early stage lung adenocarcinoma.
Abstract Surgical resection is the only curative treatment for patients with early stage non-small cell lung cancer. However, approximately 33% of non-small cell lung cancer patients recur with the stage I disease, which may be attributed to a deficiency in antitumor immunity. In the present study, for early stage lung adenocarcinoma patients with early recurrence and early non-recurrence, we investigated the quantity of tumor-infiltrating T and B cells by immunohistochemistry, as well as the genes in the complementarity determining region 3 of the T-cell receptor β chain and the B-cell receptor immunoglobuli...
Source: Experimental Cell Research - June 12, 2020 Category: Cytology Authors: Liu J, Yang X, Lu X, Zhang L, Luo W, Cheng Y, Zhang L, Yang Y, Dai N, Xu Y, Guan Y, Xiao H, Li M, Wang D Tags: Exp Cell Res Source Type: research

Introducing www.axolotl-omics.org - an integrated -omics data portal for the axolotl research community.
Abstract Genomic resources are indispensable for biological investigations in model organisms. In recent years, a number of genomic resources including a full genome assembly, extensive transcriptomic data, as well as genome editing has been developed for the axolotl, a classical model organism for developmental, neurobiological and regeneration studies, making the axolotl a highly versatile system. Here we describe the Axolotl-omics website that allows rapid ortholog searches, and access to genome and transcriptomic resources. PMID: 32540400 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 12, 2020 Category: Cytology Authors: Nowoshilow S, Tanaka EM Tags: Exp Cell Res Source Type: research

Inhibiting role of long non-coding RNA LINC01197 in inflammation in rheumatoid arthritis through the microRNA-150/THBS2 axis.
CONCLUSION: The current study suggested that LINC01197 sponged miR-150 to promote THBS2 expression, leading to TLR4/NF-κB inactivation, and ameliorated RA inflammation. These findings may offer new insights into RA treatment. PMID: 32540401 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 12, 2020 Category: Cytology Authors: Zhao F, Dong J, Guo J, Bi L Tags: Exp Cell Res Source Type: research

An introduction to the special issue on: "Na ïve pluripotency in non-rodent species: from embryos to pluripotent stem cell lines".
An introduction to the special issue on: "Naïve pluripotency in non-rodent species: from embryos to pluripotent stem cell lines". Exp Cell Res. 2020 Jun 12;:112147 Authors: Savatier P PMID: 32540402 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 12, 2020 Category: Cytology Authors: Savatier P Tags: Exp Cell Res Source Type: research

Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis.
Abstract Circular RNAs (circRNAs), a special type of non-coding RNA molecules, have been addressed to be implicated in gastric cancer progression. The GSE93541 and GSE83521 microarrays found hsa-circRNA-000670 (hsa-circ-0000670) as an up-regulated circRNAin gastric cancer. We mainly investigated the function and molecular mechanisms of hsa-circ-0000670 involved in gastric cancer. The expression of hsa-circ-0000670 was determined by RT-qPCR to be highly expressed in gastric cancer tissues relative to corresponding adjacent normal tissues, as well as in gastric cancer cell lines relative to normal gastric mucosal ep...
Source: Experimental Cell Research - June 11, 2020 Category: Cytology Authors: Liu P, Cai S, Li N Tags: Exp Cell Res Source Type: research

MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF- β/Smad/TLR3 pathway via reducing Tgfbr1.
CONCLUSION: miR-183 inhibited the expression of Tgfbr1 by direct targeting to disrupt the TGF-β/Smad/TLR3 pathway, thus repressing renal fibrosis and the secretion of inflammatory factors in LN. PMID: 32535034 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 11, 2020 Category: Cytology Authors: Qi H, Cao Q, Liu Q Tags: Exp Cell Res Source Type: research

Crk1/2 and CrkL play critical roles in maintaining podocyte morphology and function.
In this study, specific knocking down of Crk1/2 and CrkL in podocytes caused abnormal cell morphology, actin cytoskeleton rearrangement and dysfunction in cell adhesion, spreading, migration, and viability. The p130Cas, focal adhesion kinase, phosphatidylinositol 3-kinase/Akt, p38 and JNK signaling pathways involved in these alterations. Furthermore, knocking down CrkL alone conferred a more modest phenotype than did the Crk1/2 knockdown and the double knockdown. Kidney biopsy specimens from patients with focal segmental glomerulosclerosis and minimal change nephropathy showed downregulation of Crk1/2 and CrkL in glomeruli...
Source: Experimental Cell Research - June 11, 2020 Category: Cytology Authors: Du J, Meng L, Pang L, Jin B, Duan N, Huang C, Huang H, Li H Tags: Exp Cell Res Source Type: research

Knockdown of circ_0003340 induces cell apoptosis, inhibits invasion and proliferation through miR-564/TPX2 in esophageal cancer cells.
Abstract Circular RNA (circRNA) is a promising biomarker of cancer occurrence and development. The different expression levels of circRNAs in various cancers also make them possible therapeutic targets. In this work, we researched the function and underlying mechanisms of circ_0003340 (circ3340) in esophageal cancer EC1 and EC9706 cells. Firstly, we found the expression levels of circ3340 are higher in ESCC and two esophageal cancer cells than in adjacent normal tissues and Het-1a cells. Bioinformatics analysis showed circ3340 has a binding site with miR-564. This was verified by luciferase assay, which revealed...
Source: Experimental Cell Research - June 11, 2020 Category: Cytology Authors: Hou Y, Liu H, Pan W Tags: Exp Cell Res Source Type: research

MiR-30e-5p is sponged by Kcnq1ot1 and represses Angiotensin II-induced hypertrophic phenotypes in cardiomyocytes by targeting ADAM9.
In conclusion, Kcnq1ot1 sequestered miR-30e-5p to release ADAM9 to facilitate cardiac hypertrophy, indicating that Kcnq1ot1 might be used as a potentially therapeutic target for cardiac hypertrophy. PMID: 32535037 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 11, 2020 Category: Cytology Authors: Wang W, Wu C, Ren L, Bao Y, Han Y, Li C, Li Y Tags: Exp Cell Res Source Type: research

The PI3K/AKT pathway promotes fracture healing through its crosstalk with Wnt/ β-catenin.
The PI3K/AKT pathway promotes fracture healing through its crosstalk with Wnt/β-catenin. Exp Cell Res. 2020 Jun 10;:112137 Authors: Dong J, Xu X, Zhang Q, Yuan Z, Tan B Abstract PI3K/AKT is one of the key pathways that regulate cell behaviors including apoptosis, proliferation, and differentiation. Although previous studies have demonstrated that this pathway is a crucial regulator of osteoblasts, the role of PI3K/AKT in fracture healing remains unclear. It is well known that the Wnt/β-catenin pathway plays an essential role in bone regeneration. However, whether there exists crosstalk betwe...
Source: Experimental Cell Research - June 10, 2020 Category: Cytology Authors: Dong J, Xu X, Zhang Q, Yuan Z, Tan B Tags: Exp Cell Res Source Type: research

The miR-218/GAB2 axis regulates proliferation, invasion and EMT via the PI3K/AKT/GSK-3 β pathway in prostate cancer.
In this study, we determined that miR-218 also had a negative effect on cell growth, migration, and invasion ability in PCa. Our data showed that miR-218 bound to the Grb2-associated binding protein 2 (GAB2) 3'-UTR region and inhibited GAB2 expression. As a novel downstream target of miR-218, GAB2 has been reported to be involved in the occurrence and development of various human tumors, but its role in the progression and metastasis of PCa has not been addressed. We demonstrated for the first time that the expression of GAB2 in the PCa cell lines was increased, while knocking down GAB2 significantly inhibited cell growth,...
Source: Experimental Cell Research - June 6, 2020 Category: Cytology Authors: Tian J, Zhang H, Mu L, Wang M, Li X, Zhang X, Xie E, Ma M, Wu D, Du Y Tags: Exp Cell Res Source Type: research

Role of lncRNAHCP5/microRNA-525-5p/PRC1 crosstalk in the malignant behaviors of ovarian cancer cells.
CONCLUSION: The study suggested that lncRNA HCP5 might promote malignant behaviors of OC cells through the miR-525-5p/PRC1 crosstalk and the Wnt/β-catenin pathway. Silencing of HCP5 might serve as a novel option for OC treatment. PMID: 32511950 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - June 5, 2020 Category: Cytology Authors: Wang L, He M, Fu L, Jin Y Tags: Exp Cell Res Source Type: research

Renin angiotensin system inhibition attenuates adipocyte-breast cancer cell interactions.
Abstract Obesity is a significant breast cancer (BC) risk factor and is associated with 20-40% increased risk in obese post-menopausal women compared to their lean counterparts. Several obesity-related metabolic dysregulations have been linked to BC risk, including overactivation of the renin-angiotensin system (RAS). Currently, RAS inhibitors including angiotensin converting enzyme inhibitor (ACEi) and AT1 receptor blockers (ARBs), are used as safe and effective anti-hypertensive therapies in BC patients. However, it is uncertain how inhibition of RAS in adipose tissue impacts obesity-BC crosstalk. We hypothesize...
Source: Experimental Cell Research - June 3, 2020 Category: Cytology Authors: Rasha F, Ramalingam L, Menikdiwela K, Hernandez A, Moussa H, Gollahon L, Rahman RL, Moustaid-Moussa N Tags: Exp Cell Res Source Type: research

Centromere studies in the era of 'telomere-to-telomere' genomics.
Abstract We are entering into an exciting era of genomics where truly complete, high-quality assemblies of human chromosomes are available end-to-end, or from 'telomere-to-telomere' (T2T). This technological advance offers a new opportunity to include endogenous human centromeric regions in high-resolution, sequence-based studies. These emerging reference maps are expected to reveal a new functional landscape in the human genome, where centromere proteins, transcriptional regulation, and spatial organization can be examined with base-level resolution across different stages of development and disease. Such studies...
Source: Experimental Cell Research - June 3, 2020 Category: Cytology Authors: Miga KH Tags: Exp Cell Res Source Type: research

MUC16 C-terminal binding with ALDOC disrupts the ability of ALDOC to sense glucose and promotes gallbladder carcinoma growth.
In this study, we found that MUC16c promoted glucose uptake and glycolysis for GBC cell proliferation. Mass spectrometry analysis suggested that MUC16c could combine with aldolase. The ALDOC mRNA and protein are overexpressed in GBC tumors. The IHC results also showed the consistent up-regulation of. ALDOC and MUC16c level in GBC tumor tissues than in peritumor tissues. We determined that MUC16c combining with ALDOC promoted ALDOC protein stability and disrupted the ability of ALDOC sensing glucose deficiency, which activated AMPK pathway and increased GBC cell proliferation. ALDOC knockdown significantly inhibited the glu...
Source: Experimental Cell Research - June 2, 2020 Category: Cytology Authors: Fan K, Wang J, Sun W, Shen S, Ni X, Gong Z, Zheng B, Gao Z, Ni X, Suo T, Liu H, Liu H Tags: Exp Cell Res Source Type: research

A novel methodology of the myeloid-derived suppressor cells (MDSCs) generation with splenic stroma feeder cells.
Abstract Myeloid-derived suppressor cells (MDSCs) are a significant obstacle for immunotherapy of cancer. It is of great clinical relevance to study the mechanism of MDSCs accumulation in mouse spleens and establish a stable method to obtain high-purity MDSCs in vitro for further research. Here, we established a new method for amplifying a large number of highly pure MDSCs in vitro. To mimic the microenvironment of MDSCs development in vivo, mouse splenic stroma feeder cells and serum-free medium containing granulocyte-macrophage colony stimulating factor (GM-CSF) were used to induce myeloid precursors in mouse bo...
Source: Experimental Cell Research - May 30, 2020 Category: Cytology Authors: Tian F, Jiang P, Wu M, Xing Y, Liu L, Dai X, Tang H, Yang ZF, Wang L, Zhu X Tags: Exp Cell Res Source Type: research

Resveratrol binds and inhibits transcription factor HIF-1 α in pancreatic cancer.
Resveratrol binds and inhibits transcription factor HIF-1α in pancreatic cancer. Exp Cell Res. 2020 May 30;:112126 Authors: Srivani G, Behera SK, Dariya B, Aliya S, Alam A, Nagaraju GP Abstract Hypoxia-inducible factor-1 alpha (HIF-1α) has been recognized as one of the essential regulators that is expressed in greater levels in pancreatic cancer (PC) and is connected with poor prognosis. Resveratrol was identified as a natural compound with many biological functions, with anti-inflammatory, antioxidant, and anticancer effects that inhibit the proliferation and progression of PC cells cause...
Source: Experimental Cell Research - May 30, 2020 Category: Cytology Authors: Srivani G, Behera SK, Dariya B, Aliya S, Alam A, Nagaraju GP Tags: Exp Cell Res Source Type: research

A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries.
Abstract Acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome (ARDS) are common lung disorders characterized by alveolar-capillary barrier disruption and dyspnea, which can cause substantial morbidity and mortality. Currently, a cluster of acute respiratory illnesses, known as novel coronavirus (2019-nCoV)-infected pneumonia (NCIP), which allegedly originally occurred in Wuhan, China, has increased rapidly worldwide. The critically ill patients with ARDS have high mortality in subjects with comorbidities. Previously, the excessive recruitment and activation of neutrophils (polymorp...
Source: Experimental Cell Research - May 28, 2020 Category: Cytology Authors: Lv D, Xu Y, Cheng H, Ke Y, Zhang X, Ying K Tags: Exp Cell Res Source Type: research

Extracellular vesicle encapsulated microRNA-320a inhibits endometrial cancer by suppression of the HIF1 α/VEGFA axis.
Extracellular vesicle encapsulated microRNA-320a inhibits endometrial cancer by suppression of the HIF1α/VEGFA axis. Exp Cell Res. 2020 May 27;:112113 Authors: Zhang N, Wang Y, Liu H, Shen W Abstract Accumulating evidence indicates that cancer-associated fibroblasts (CAFs) play a crucial role in endometrial cancer (EC) pathogenesis. The present study investigated the clinical significance and biological function of extracellular vesicle (EV) encapsulated miR-320a released from CAFs in EC. EC-related microarray data was obtained from the GSE25405 database and differential analysis was performed. ...
Source: Experimental Cell Research - May 27, 2020 Category: Cytology Authors: Zhang N, Wang Y, Liu H, Shen W Tags: Exp Cell Res Source Type: research

Shikonin relieves osteoporosis of ovariectomized mice by inhibiting RANKL-induced NF- κB and NFAT pathways.
Shikonin relieves osteoporosis of ovariectomized mice by inhibiting RANKL-induced NF-κB and NFAT pathways. Exp Cell Res. 2020 May 27;:112115 Authors: Chen Y, Zhong X, Zhang Y, Xia C, Yang M, Hu X Abstract Postmenopausal osteoporosis is very common in women. Currently, many kinds of new drugs are being developed for this disease. Postmenopausal osteoporosis is closely related to overactivity of osteoclasts in body. Shikonin is purple red naphthoquinone pigment extracted from lithospermum, which has anti-inflammation, antivirus, anticancer and other bioactivities. At the same time, it has been pro...
Source: Experimental Cell Research - May 27, 2020 Category: Cytology Authors: Chen Y, Zhong X, Zhang Y, Xia C, Yang M, Hu X Tags: Exp Cell Res Source Type: research

Cellular stress orchestrates the localization of hnRNP H to stress granules.
Abstract Heterogeneous nuclear ribonucleoprotein (hnRNP) H is a member of hnRNP H/F protein subfamily of hnRNPs that regulate the maturation and post-transcriptional processing of pre-mRNA. As a component of an mRNA export complex, hnRNP H shuttles mature mRNA from the nucleus to the cytoplasm. Although hnRNP H is primarily a nuclear protein, it can accumulate in the cytoplasm in certain tissues and cell types; however, the physiological relevance of hnRNP H cytoplasmic accumulation is unknown. Here we show that under cellular stress hnRNP H accumulates in the cytoplasm and is required for efficient recovery from ...
Source: Experimental Cell Research - May 27, 2020 Category: Cytology Authors: Wall ML, Bera A, Wong FK, Lewis SM Tags: Exp Cell Res Source Type: research

The anti-apoptotic proteins Bcl-2 and Bcl-xL suppress Beclin1/Atg6-mediated lethal autophagy in polyploid cells.
Abstract Inhibition of Aurora-B kinase is a synthetic lethal therapy for tumors that overexpress the MYC oncoprotein. It is currently unclear whether co-occurring oncogenic alterations might influence this synthetic lethality by conferring more or less potency in the killing of tumor cells. To identify such modifiers, isogenic cell lines were utilized to test a variety of cancer genes that have been previously demonstrated to promote survival under conditions of cellular stress, contribute to chemoresistance and/or suppress MYC-primed apoptosis. It was found that Bcl-2 and Bcl-xL, two antiapoptotic members of the ...
Source: Experimental Cell Research - May 27, 2020 Category: Cytology Authors: Zhang J, Zhang S, Shi Q, Allen TD, You F, Yang D Tags: Exp Cell Res Source Type: research

Retraction notice to "One isoform of Arg tyrosine kinase is nuclear and the other seven cytosolic isoforms differently modulate cell morphology, motility and the cytoskeleton" [Experimental Cell Research 319 (2013) 2091-2102].
Retraction notice to "One isoform of Arg tyrosine kinase is nuclear and the other seven cytosolic isoforms differently modulate cell morphology, motility and the cytoskeleton" [Experimental Cell Research 319 (2013) 2091-2102]. Exp Cell Res. 2020 May 26;:112044 Authors: Bianchi C, Torsello B, Di Stefano V, Zipeto MA, Facchetti R, Bombelli S, Peregon RA PMID: 32471682 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 26, 2020 Category: Cytology Authors: Bianchi C, Torsello B, Di Stefano V, Zipeto MA, Facchetti R, Bombelli S, Peregon RA Tags: Exp Cell Res Source Type: research

UCP2 silencing in glioblastoma reduces cell proliferation and invasiveness by inhibiting p38  MAPK pathway.
UCP2 silencing in glioblastoma reduces cell proliferation and invasiveness by inhibiting p38 MAPK pathway. Exp Cell Res. 2020 May 26;:112110 Authors: Wu S, Luo C, Hameed NUF, Wang Y, Zhuang D Abstract Uncoupling protein-2 (UCP2) is a mitochondrial inner membrane anion carrier and is emerging as a negative regulator of ROS production. Overexpression of UCP2 has been detected in various tumors, but its role in glioblastoma remains unclear. Using tissue microarrays and interrogations of public databases, we explored that the expression of UCP2 is upregulated in glioma, especially in GBM, and overexpres...
Source: Experimental Cell Research - May 26, 2020 Category: Cytology Authors: Wu S, Luo C, Hameed NUF, Wang Y, Zhuang D Tags: Exp Cell Res Source Type: research

Exosomal miR-532-5p from bone marrow mesenchymal stem cells reduce intervertebral disc degeneration by targeting RASSF5.
In this study, we found that exosomes derived from BMSCs (BMSCs-Exos) inhibited the apoptotic rates, extracellular matrix (ECM) degradation, and fibrosis deposition in TNF-α-induced nucleus pulposus cells (NPCs). Importantly, the level of miR-532-5p was observed to be decreased in apoptotic NPCs, but abundant in BMSCs-Exos with TNF-α treatment. The results showed that BMSCs-Exos under TNF-α stimuli exerted better effects on NPCs than BMSCs-Exos, which might be mitigated by the inhibition of miR-532-5p in BMSCs-Exos. The gain-of-function results suggested that the direct overexpression of miR-532-5p in NPC...
Source: Experimental Cell Research - May 25, 2020 Category: Cytology Authors: Zhu G, Yang X, Peng C, Yu L, Hao Y Tags: Exp Cell Res Source Type: research

The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.
Abstract Regulating proteasome activity is a potent therapeutic aspect of age-related hearing loss, which has been proven to protect neurons from age-related damaging. PSMD11, subunit of the 19S proteasome regulatory particle, is known to mainly up-regulate proteasome activity and prolong aging. However, the mechanism of PSMD11 in age-related hearing loss has not been deeply explored. In the present study, we explore the function and mechanism of PSMD11 protecting neurons in d-Galactose (D-Gal) mimetic aging models. Age-related pathologies were detected by Taq-PCR, ABR, Transmission electron microscopy, toluidine ...
Source: Experimental Cell Research - May 22, 2020 Category: Cytology Authors: Wu H, Sun H, He Z, Chen X, Li Y, Zhao X, Kong W, Kong W Tags: Exp Cell Res Source Type: research

Corrigendum to "The long non-coding RNA HOTAIR promotes the proliferation of serious ovarian cancer cells through the regulation of cell cycle arrest and apoptosis" [Exp. Cell Res. 333 (2015) 238-248].
Corrigendum to "The long non-coding RNA HOTAIR promotes the proliferation of serious ovarian cancer cells through the regulation of cell cycle arrest and apoptosis" [Exp. Cell Res. 333 (2015) 238-248]. Exp Cell Res. 2020 May 21;:112035 Authors: Qiu JJ, Wang Y, Ding JX, Jin HY, Yang G, Hua KQ PMID: 32448483 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 21, 2020 Category: Cytology Authors: Qiu JJ, Wang Y, Ding JX, Jin HY, Yang G, Hua KQ Tags: Exp Cell Res Source Type: research

Stanniocalcin 2 contributes to aggressiveness and is a prognostic marker for oral squamous cell carcinoma.
This study aimed to access the prognostic value of STC2 in oral squamous cell carcinoma (OSCC) and its implications in oral tumorigenesis. STC2 expression was examined in 2 independent cohorts of OSCC tissues by immunohistochemistry. A loss-of-function strategy using shRNA targeting STC2 was employed to investigate STC2 in vitro effects on proliferation, apoptosis, migration, invasion, epithelial-mesenchymal transition (EMT) and possible activation of signaling pathways. Moreover, STC2 effects were assessed in vivo in a xenograft mouse cancer model. High expression of STC2 was significantly associated with poor disease-spe...
Source: Experimental Cell Research - May 20, 2020 Category: Cytology Authors: Ferreira do Carmo A, Dourado MR, Ervolino de Oliveira C, Bastos DC, Domingueti CB, Ribeiro Paranaíba LM, Sawazaki-Calone Í, Borges GÁ, Silva Guerra EN, Casarin RC, Graner E, Salo TA, de Almeida Freitas R, Galvão HC, Coletta RD Tags: Exp Cell Res Source Type: research

FGF21 mitigates atherosclerosis via inhibition of NLRP3 inflammasome-mediated vascular endothelial cells pyroptosis.
Abstract Fibroblast growth factor 21(FGF21) is an endocrine cytokine that targets inflammation and atherosclerosis (AS). However, the underlying molecular mechanisms of the FGF21 anti-AS effect remain to be explored. Pyroptosis induced by hyperlipidemia or oxidized low-density lipoprotein (oxLDL) in vascular endothelial cells (VECs) is a significant step in the advancement of AS. This work aimed to evaluate the mechanisms and functioning of FGF21 against AS using an atherosclerotic animal model and oxLDL mimic in vitro. We found that exogenous treatments with FGF21 significantly reduced the aortic sinus plaque are...
Source: Experimental Cell Research - May 20, 2020 Category: Cytology Authors: Zeng Z, Zheng Q, Chen J, Tan X, Li Q, Ding L, Zhang R, Lin X Tags: Exp Cell Res Source Type: research

FUT7 promotes the malignant transformation of follicular thyroid carcinoma through α1,3-Fucosylation of EGF receptor.
This study investigated the role of fulcosytransferase VII (FUT7) in the progression of follicular thyroid carcinoma (FTC). FUT7 expression was found to be upregulated in FTC compared to paracancerous thyroid tissue, and in FTC with T2 stage of TMN classification compared to FTC with T1 stage. FUT7 overexpression promoted cell proliferation, epithelial-mesenchymal transition (EMT), and the migration and invasion of primary FTC cell line FTC-133. Consistently, FUT7 knock-down inhibited cell proliferation, EMT, as well as the migration and invasion of the metastatic FTC cell line FTC-238. Mechanistic investigation revealed t...
Source: Experimental Cell Research - May 19, 2020 Category: Cytology Authors: Qin H, Liu J, Yu M, Wang H, Thomas AM, Li S, Yan Q, Wang L Tags: Exp Cell Res Source Type: research

USP9X prevents AGEs-induced upregulation of FN and TGF- β1 through activating Nrf2-ARE pathway in rat glomerular mesangial cells.
USP9X prevents AGEs-induced upregulation of FN and TGF-β1 through activating Nrf2-ARE pathway in rat glomerular mesangial cells. Exp Cell Res. 2020 May 19;:112100 Authors: Huang K, Zhao X Abstract Oxidative stress is a key pathological factor for diabetic renal fibrosis by activating TGF-β/Smad pathway in glomerular mesangial cells (GMCs) to promote the synthesis of extracellular matrix such as fibronectin (FN). Nuclear factor-E2-related factor (Nrf2)- anti-oxidant response element (ARE) anti-oxidative pathway has crucial renoprotective effects, and inhibiting ubiquitin-mediated degradation ...
Source: Experimental Cell Research - May 19, 2020 Category: Cytology Authors: Huang K, Zhao X Tags: Exp Cell Res Source Type: research

Antagomirs targeting miR-142-5p attenuate pilocarpine-induced status epilepticus in mice.
Abstract MicroRNAs (miRNAs) are reported to involve in pathogenesis of temporal lobe epilepsy (TLE). miR-142-5p is found increased in TLE, but its role remains unknown. In the study, we established a mouse model of status epilepticus (SE) with pilocarpine and a cell model of TLE. Quantitative real-time PCR revealed an up-regulation of miR-142-5p and down-regulation of mitochondrial Rho 1 (Miro1) in the mouse mode of SE. Administration of miR-142-5p antagomirs via intracerebroventricular injection attenuated pilocarpine-induced SE and hippocampal damage, and alleviated mitochondrial dysfunction along with increased...
Source: Experimental Cell Research - May 18, 2020 Category: Cytology Authors: Zhang H, Lian Y, Xie N, Cheng X, Chen C, Xu H, Zheng Y Tags: Exp Cell Res Source Type: research

Automated cell cluster analysis provides insight into multi-cell-type interactions between immune cells and their targets.
Abstract Understanding interactions between immune cells and their targets is an important step on the path to fully characterizing the immune system, and in doing so, learning how it combats disease. Many studies of these interactions have a narrow focus, often looking only at a binary result of whether or not a specific treatment was successful or only focusing on the interactions between two individual cells. Therefore, in an effort to more comprehensively study multicellular interactions among immune cells and their targets, we used in vitro longitudinal time-lapse imaging and developed an automated cell clust...
Source: Experimental Cell Research - May 18, 2020 Category: Cytology Authors: Diehl MI, Wolf SP, Bindokas VP, Schreiber H Tags: Exp Cell Res Source Type: research

IL-6 promotes cell adhesion in human endothelial cells via microRNA-126-3p suppression.
Abstract Atherosclerosis is an important underlying cause of cardiovascular diseases; vascular endothelial cells play a vital role in inflammatory responses in the initial steps of atherosclerosis. High levels of the pro-inflammatory cytokine interleukin-6 (IL-6) long have been considered a risk factor in the development and complications of atherosclerotic disease. However, it is still controversial whether IL-6 is atherogenic or atheroprotective. Recently, miR-126-3p, an endothelial cell-specific microRNA, has been proposed as an atheroprotective molecule. Therefore, we investigated whether IL-6 accelerates endo...
Source: Experimental Cell Research - May 18, 2020 Category: Cytology Authors: Ohta M, Kihara T, Toriuchi K, Aoki H, Iwaki S, Kakita H, Yamada Y, Aoyama M Tags: Exp Cell Res Source Type: research

Methyl-CpG-binding domain 3 inhibits stemness of pancreatic cancer cells via Hippo signaling.
Abstract Methyl-CpG-binding domain 3 (MBD3), as an induced stem cells reprogramming barrier, has an abnormal expression in various prevalent malignancies. However, in pancreatic cancer cell stemness, the roles of MBD3 remain unclear. In our study, the effects of MBD3 were investigated on the proliferation, stemness and the underlying mechanism in pancreatic cancer cells. Firstly, MBD3 knockdown was proved to promote proliferation and sphere formation of pancreatic cancer cells and tumorigenesis, while MBD3 upregulation inhibited the above results. Also, MBD3 downregulation notably increased stemness markers level ...
Source: Experimental Cell Research - May 15, 2020 Category: Cytology Authors: Wang H, Feng W, Chen W, He J, Min J, Liu Y, Li F, Chen J, Wu S, Chen B, Gong A, Xu M Tags: Exp Cell Res Source Type: research

Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9.
Abstract The relationship between Lamin B2 and tumor proliferation and migration is unclear. We explored the impact of Lamin B2 on non-small cell lung cancer (NSCLC) cells. Tissue microarray and immunohistochemistry were combined to evaluate Lamin B2 expression and its relationship with the clinicopathological factors found in NSCLC. Western blotting, immunofluorescence analysis, and bioinformatics were used to investigate the effects of Lamin B2 on various regulatory pathways in cancer. Cytological experiments were conducted to evaluate Lamin B2 expression in tumor cells. We conducted co-immunoprecipitation and c...
Source: Experimental Cell Research - May 13, 2020 Category: Cytology Authors: Zhang MY, Han YC, Han Q, Liang Y, Luo Y, Wei L, Yan T, Yang Y, Liu SL, Wang EH Tags: Exp Cell Res Source Type: research

iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction.
CONCLUSION: Suppression of ACE and AP-N expression mediates congenital UPJO pathogenesis by repressing renal tubular epithelial proliferation, promoting ROS production, and enhancing inflammatory factor expression. PMID: 32416091 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 13, 2020 Category: Cytology Authors: Liu R, Zhang W, Luo M, Qin X, Yang F, Wei Q Tags: Exp Cell Res Source Type: research

High mobility group AT-hook 2 promotes tumorigenicity of pancreatic cancer cells via upregulating ANLN.
This study uncovered the mechanism of HMGA2-mediated regulation of tumorigenicity in pancreatic cancer. We showed that HMGA2 was highly expressed in pancreatic cancer cells and correlated with poor prognosis. HMGA2 expression knockdown inhibited the tumorigenicity of pancreatic cancer cells. Conversely, overexpression of HMGA2 promoted tumorigenicity. Combination of ChIP-Seq, RNA-Seq and dual-luciferase reporter assays revealed HMGA2 could directly regulate ANLN expression. Furthermore, we found ANLN could mediate the HMGA2-induced effects on pancreatic cancer cells. The identification of the regulatory mechanism of HMGA2 ...
Source: Experimental Cell Research - May 12, 2020 Category: Cytology Authors: Guo HH, Wang YZ, Zhang ZK, Li MZ, Tian XD, Yang YM Tags: Exp Cell Res Source Type: research