12,13-diHOME attenuates high glucose-induced calcification of vascular smooth muscle cells through repressing CPT1A-mediated HMGB1 succinylation
This study enrolled 159 elderly patients with type 2 diabetes and divided them into three groups, T1, T2 and T3, according to brachial-ankle pulse wave velocity(BaPWV). There were statistically significant differences in the waist circumference, waist hip ratio, systolic blood pressure, 12,13-diHOME (a lipokin) concentration among T1, T2 and T3. 12,13-diHOME levels were positively correlated to high density lipoprotein cholesterol and total cholesterol, but negatively correlated to with waist circumference, waist hip ratio, systolic blood pressure and baPWV. Studies in vitro showed that 12,13-diHOME effectively inhibits ca...
Source: Experimental Cell Research - April 14, 2024 Category: Cytology Authors: Huahua Li Lingling Zou Jin Zheng Tao Yang Source Type: research
Inactivation of VRK1 Sensitizes Ovarian Cancer to PARP Inhibition through regulating DNA-PK stability
Exp Cell Res. 2024 Apr 11:114036. doi: 10.1016/j.yexcr.2024.114036. Online ahead of print.ABSTRACTOvarian cancer is the leading cause of gynecologic cancer death. Among the most innovative anti-cancer approaches, the genetic concept of synthetic lethality is that mutations in multiple genes work synergistically to effect cell death. Previous studies found that although vaccinia-related kinase-1 (VRK1) associates with DNA damage repair proteins, its underlying mechanisms remain unclear. Here, we found high VRK1 expression in ovarian tumors, and that VRK1 depletion can significantly promote apoptosis and cell cycle arrest. T...
Source: Experimental Cell Research - April 13, 2024 Category: Cytology Authors: Do Yeon Kim Hyeseon Yun Ji-Eun You Ji-U Lee Dong-Hee Kang Yea Seong Ryu Dong-In Koh Dong-Hoon Jin Source Type: research
Ketohexokinase-A deficiency attenuates the proliferation via reducing β-catenin in gastric cancer cells
Exp Cell Res. 2024 Apr 11:114038. doi: 10.1016/j.yexcr.2024.114038. Online ahead of print.ABSTRACTOverconsumption of fructose is closely related to cancer. Ketohexokinase (KHK) catalyzes the conversion from fructose to fructose-1-phosphate (F1P), which is the first and committed step of fructose metabolism. Recently, aberrant KHK activation has been identified in multiple malignancies. However, the roles of KHK in gastric cancer (GC) cells are largely unclear. Herein, we reveal that the expression of ketohexokinase-A (KHK-A), one alternatively spliced KHK isoform that possesses low affinity for fructose, was markedly incre...
Source: Experimental Cell Research - April 13, 2024 Category: Cytology Authors: Gang Ma Siya Liu Fenglin Cai Han Liang Jingyu Deng Rupeng Zhang Mingzhi Cai Source Type: research
Ferroptosis contributes to airway epithelial E-cadherin disruption in a mixed granulocytic asthma mouse model
This study was aimed to evaluate the role of ferroptosis and to investigate whether ferroptosis mediates E-cadherin disruption in mixed granulocyte asthma (MGA). Two murine models of MGA were established using toluene diisocyanate (TDI) or ovalbumin with Complete Freund's Adjuvant (OVA/CFA). Specific antagonists of ferroptosis, including Liproxstatin-1 (Lip-1) and Ferrostatin-1 (Fer-1) were given to the mice. The allergen-exposed mice displayed markedly shrunk mitochondria in the airway epithelia, with decreased volume and denser staining accompanied by down-regulated GPX4 as well as up-regulated FTH1 and malondialdehyde, ...
Source: Experimental Cell Research - April 12, 2024 Category: Cytology Authors: Sudan Gan Liqin Lin Zemin Chen Hailing Zhang Haixiong Tang Changyun Yang Jing Li Shiyue Li Lihong Yao Source Type: research
Ferroptosis contributes to airway epithelial E-cadherin disruption in a mixed granulocytic asthma mouse model
This study was aimed to evaluate the role of ferroptosis and to investigate whether ferroptosis mediates E-cadherin disruption in mixed granulocyte asthma (MGA). Two murine models of MGA were established using toluene diisocyanate (TDI) or ovalbumin with Complete Freund's Adjuvant (OVA/CFA). Specific antagonists of ferroptosis, including Liproxstatin-1 (Lip-1) and Ferrostatin-1 (Fer-1) were given to the mice. The allergen-exposed mice displayed markedly shrunk mitochondria in the airway epithelia, with decreased volume and denser staining accompanied by down-regulated GPX4 as well as up-regulated FTH1 and malondialdehyde, ...
Source: Experimental Cell Research - April 12, 2024 Category: Cytology Authors: Sudan Gan Liqin Lin Zemin Chen Hailing Zhang Haixiong Tang Changyun Yang Jing Li Shiyue Li Lihong Yao Source Type: research
AR-V7 expression facilitates accelerated G2/M phase transition in castration-resistant prostate cancer
In this study, we have investigated the regulation of AR-V7 during cell cycle and identified a distinct pattern of periodic fluctuation, peaking during G2/M phase. This fluctuation correlates with the expression of Cdc-2 like kinase 1 (CLK1) and phosphorylated serine/arginine-rich splicing factor 1 (p-SRSF1) during these phases, pointing towards their role in AR-V7 generation. Functional assays reveal that CLK1 knockdown prolongs the S phase, leading to altered cell cycle distribution and increased accumulation of AR-V7 and pSRSF1 in G1/S phase. Conversely, CLK1 overexpression rescues AR-V7 and p-SRSF1 levels in the G2/M p...
Source: Experimental Cell Research - April 11, 2024 Category: Cytology Authors: Taruna Saini Parth Gupta Rajnikant Raut Vinayak Nayak Pabbithi Bharathnaveen Parul Mishra Ashish Misra Source Type: research
AR-V7 expression facilitates accelerated G2/M phase transition in castration-resistant prostate cancer
In this study, we have investigated the regulation of AR-V7 during cell cycle and identified a distinct pattern of periodic fluctuation, peaking during G2/M phase. This fluctuation correlates with the expression of Cdc-2 like kinase 1 (CLK1) and phosphorylated serine/arginine-rich splicing factor 1 (p-SRSF1) during these phases, pointing towards their role in AR-V7 generation. Functional assays reveal that CLK1 knockdown prolongs the S phase, leading to altered cell cycle distribution and increased accumulation of AR-V7 and pSRSF1 in G1/S phase. Conversely, CLK1 overexpression rescues AR-V7 and p-SRSF1 levels in the G2/M p...
Source: Experimental Cell Research - April 11, 2024 Category: Cytology Authors: Taruna Saini Parth Gupta Rajnikant Raut Vinayak Nayak Pabbithi Bharathnaveen Parul Mishra Ashish Misra Source Type: research
The CREB1/WNK1 axis promotes the tumorigenesis of ovarian cancer via regulating HIF-1
Exp Cell Res. 2024 Apr 8:114006. doi: 10.1016/j.yexcr.2024.114006. Online ahead of print.ABSTRACTThe aim of this study was to explore the functions and molecular mechanisms of the WNK lysine deficient protein kinase 1 (WNK1) in the development of ovarian cancer. Firstly, loss- and gain-of-function assays were carried out and subsequently cell proliferation, apoptosis, invasion and migration were detected. Furthermore, WNK1 action on glucose uptake, lactate production and adenosine triphosphate (ATP) level were assessed. The roles of WNK1 on cisplatin resistance were explored using CCK-8, colony formation, and flow cytometr...
Source: Experimental Cell Research - April 10, 2024 Category: Cytology Authors: Yifei Ma Hui Zong Pan Pan Hui Shang Xingsheng Yang Source Type: research
Development and in-depth characterization of BRAFi-resistant melanoma cell lines in vitro and in vivo
Exp Cell Res. 2024 Apr 7;438(1):114033. doi: 10.1016/j.yexcr.2024.114033. Online ahead of print.ABSTRACTRegardless of the clinical response and improved patient survival observed following treatment with BRAFi like Vemurafenib (Vem), rapid development of resistance still remains as a major obstacle in melanoma therapy. In this context, we developed and characterized two acquired Vem-resistant melanoma cell lines, A375V and SK-MEL-28V, and an intrinsically Vem-resistant cell line, RPMI-7951. Altered morphology and growth rate of the resistant cell lines displayed spindle-shaped cells with filopodia formation and enhanced pr...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Aishwarya Saraswat Ketan Patel Source Type: research
Role of ONECUT family transcription factors in cancer and other diseases
Exp Cell Res. 2024 Apr 7:114035. doi: 10.1016/j.yexcr.2024.114035. Online ahead of print.ABSTRACTMembers of ONECUT transcription factor play an essential role in several developmental processes, however, the atypical expression of ONECUT proteins lead to numerous diseases, including cancer. ONECUT family proteins promote cell proliferation, progression, invasion, metastasis, angiogenesis, and stemness. This family of proteins interacts with other proteins such as KLF4, TGF-β, VEGFA, PRC2, SMAD3 and alters their expression involved in the regulation of various signaling pathways including Jak/Stat3, Akt/Erk, TGF-β, Smad2/...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Kumari Sunita Prajapati Sanjay Gupta Smriti Choudhari Shashank Kumar Source Type: research
Development and in-depth characterization of BRAFi-resistant melanoma cell lines in vitro and in vivo
Exp Cell Res. 2024 Apr 7:114033. doi: 10.1016/j.yexcr.2024.114033. Online ahead of print.ABSTRACTRegardless of the clinical response and improved patient survival observed following treatment with BRAFi like Vemurafenib (Vem), rapid development of resistance still remains as a major obstacle in melanoma therapy. In this context, we developed and characterized two acquired Vem-resistant melanoma cell lines, A375V and SK-MEL-28V, and an intrinsically Vem-resistant cell line, RPMI-7951. Altered morphology and growth rate of the resistant cell lines displayed spindle-shaped cells with filopodia formation and enhanced prolifera...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Aishwarya Saraswat Ketan Patel Source Type: research
Role of ONECUT family transcription factors in cancer and other diseases
Exp Cell Res. 2024 Apr 7:114035. doi: 10.1016/j.yexcr.2024.114035. Online ahead of print.ABSTRACTMembers of ONECUT transcription factor play an essential role in several developmental processes, however, the atypical expression of ONECUT proteins lead to numerous diseases, including cancer. ONECUT family proteins promote cell proliferation, progression, invasion, metastasis, angiogenesis, and stemness. This family of proteins interacts with other proteins such as KLF4, TGF-β, VEGFA, PRC2, SMAD3 and alters their expression involved in the regulation of various signaling pathways including Jak/Stat3, Akt/Erk, TGF-β, Smad2/...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Kumari Sunita Prajapati Sanjay Gupta Smriti Choudhari Shashank Kumar Source Type: research
Salidroside induces mitochondrial dysfunction and ferroptosis to inhibit melanoma progression through reactive oxygen species production
This study used human malignant melanoma cells treated with SAL either alone or in combination with ROS scavenger (NAC) or ferroptosis inducer (Erastin). Through cell viability, wound healing assays, and a Seahorse analyze found that SAL inhibited cell proliferation, migration, extracellular acidification rate, and oxygen consumption rate. Metabolic flux analysis, complexes I, II, III, and IV activity of the mitochondrial respiratory chain assays, mitochondrial membrane potential assay, mitochondrial ROS, and transmission electron microscope revealed that SAL induced mitochondrial dysfunction and ultrastructural damage. As...
Source: Experimental Cell Research - April 8, 2024 Category: Cytology Authors: Xianqi Zhang Mengdi Zhang Ziyan Zhang Shengbo Zhou Source Type: research
Salidroside induces mitochondrial dysfunction and ferroptosis to inhibit melanoma progression through reactive oxygen species production
This study used human malignant melanoma cells treated with SAL either alone or in combination with ROS scavenger (NAC) or ferroptosis inducer (Erastin). Through cell viability, wound healing assays, and a Seahorse analyze found that SAL inhibited cell proliferation, migration, extracellular acidification rate, and oxygen consumption rate. Metabolic flux analysis, complexes I, II, III, and IV activity of the mitochondrial respiratory chain assays, mitochondrial membrane potential assay, mitochondrial ROS, and transmission electron microscope revealed that SAL induced mitochondrial dysfunction and ultrastructural damage. As...
Source: Experimental Cell Research - April 8, 2024 Category: Cytology Authors: Xianqi Zhang Mengdi Zhang Ziyan Zhang Shengbo Zhou Source Type: research
Effects of sevoflurane on lung alveolar epithelial wound healing and survival in a sterile in vitro model of acute respiratory distress syndrome
In this study, we investigated the effects of sevoflurane on lung injury in cultured human carcinoma-derived lung alveolar epithelial (A549) cells. We found that sevoflurane was associated with improved wound healing after exposure to inflammatory cytokines, with preserved cell proliferation but no effect on cell migration properties. Sevoflurane exposure was also associated with enhanced cell viability and active autophagy in A549 cells exposed to cytokines. These findings suggest that sevoflurane may have beneficial effects on lung epithelial injury by promoting alveolar epithelial wound healing and by influencing the su...
Source: Experimental Cell Research - April 7, 2024 Category: Cytology Authors: Florian Loubet C édric Robert Charlotte Leclaire Camille Theilli ère C écile Saint-Béat Woodys Lenga Ma Bonda Ruoyang Zhai R égine Minet-Quinard Corinne Belville Loic Blanchon Vincent Sapin Marc Garnier Matthieu Jabaudon Source Type: research
