Adipose-derived mesenchymal stem cells ameliorate acute liver injury in rat model of CLP induced-septic via sTNFR1.
This study aimed to test the hypothesis that rat adipose-derived mesenchymal stem cells (ADMSCs) produce a amount of soluble tumour necrosis factor receptor 1 (sTNFR1), which ameliorated liver injury and inflammation and increased the survival rate of septic rat model.120 adult male Sprague-Dawley rats were randomly divided into 4 groups: sham-operated (Sham), sepsis-induced by cecal ligation and puncture (CLP), shNC (injected 1 × 106 ADMSCs with transfected with scramble shRNA 1 h after CLP), and shsTNFR1 (injected 1 × 106 ADMSCs with transfected with sTNFR1 1 h after CLP). The serum sTNFR1 levels ...
Source: Experimental Cell Research - June 12, 2019 Category: Cytology Authors: Liang H, Ding X, Yu Y, Zhang H, Wang L, Kan Q, Ma S, Guan F, Sun T Tags: Exp Cell Res Source Type: research
Suppressive effect of microRNA-449a on the NDRG1/PTEN/AKT axis regulates endometrial cancer growth and metastasis.
In this study, our analysis found that miR-449a expression is inversely correlated with the stage of EC. Downregulation of miR-449a was correlated with tumor progression and poor prognosis in the EC patients. Results of functional analyses revealed that overexpression of miR-449a in human EC cells alleviated cell proliferation, invasion and metastasis. Conversely, loss of miR-449a in EC cancer cells facilitated all these cellular activities. Moreover, we identified N-myc downstream-regulated gene 1 (NDRG1) as a direct and functional target gene of miR-449a in EC cells, and the expression of NDRG1 in 87 EC specimens were ...
Source: Experimental Cell Research - June 12, 2019 Category: Cytology Authors: Wu AY, Hu Y, Cang W, Li D, Wang WJ, Tian Q, Gu LY, Zhang N, Ji F, Qiu LH Tags: Exp Cell Res Source Type: research
SIRT1 downregulated FGB expression to inhibit RCC tumorigenesis by destabilizing STAT3.
This study aimed to define the function of SIRT1 and underlying mechanism in the RCC progression. The expression of SIRT1 and FGB in RCC specimens and cells were detected by immunoblotting and immunostaining. Luciferase reporter assay was performed to confirm FGB as the target gene of STAT3. Other methods including stable transfection, co-immunoprecipitation, Western blot, and in vitro and in vivo proliferation assays were also performed. Our results showed that SIRT1 expression was downregulated in RCC tissues compared to adjacent normal tissues and relatively high expression of SIRT1 conferred a better prognosis for pati...
Source: Experimental Cell Research - June 12, 2019 Category: Cytology Authors: Chen Y, Zhu Y, Sheng Y, Xiao J, Xiao Y, Cheng N, Chai Y, Wu X, Zhang S, Xiang T Tags: Exp Cell Res Source Type: research
Role of GTPases in the regulation of mitochondrial dynamics in Parkinson's disease.
Abstract Mitochondrion is a highly dynamic organelle that undergoes frequent fusion and division, and the balance of these opposing processes regulates mitochondrial morphology, distribution, and function. Mitochondrial fission facilitates the replication and distribution of mitochondria during cell division, whereas the fusion process including inner and outer mitochondrial membrane fusion allows the exchange of intramitochondrial material between adjacent mitochondria. Despite several GTPase family proteins have been implicated as key modulators of mitochondrial dynamics, the mechanisms by which these proteins r...
Source: Experimental Cell Research - June 10, 2019 Category: Cytology Authors: Zhang X, Huang W, Fan Y, Sun Y, Ge X Tags: Exp Cell Res Source Type: research
Gab2 promotes cancer stem cell like properties and metastatic growth of ovarian cancer via downregulation of miR-200c.
In this study, we examined the effects of Gab2 expression on CSC-like cell growth using Aldefluor and tumorshpere assays commonly used for assessing ovarian cancer cells with CSC properties. Gab2 increased the number of ALDH+ cells and tumorsphere formation in two different ovarian cancer cell lines OVCAR5 and OVCAR8, whereas knockdown of Gab2 decreased the number of ALDH+cells and tumorsphere formation in Caov-3 cells. Furthermore, Gab2 promoted metastatic tumor growth of OVCAR5 in nude mice. Mechanistically, we uncovered that Gab2 via PI3K activation specifically inhibited miR-200c expression. miR-200c downregulation c...
Source: Experimental Cell Research - June 10, 2019 Category: Cytology Authors: Fang Z, Li T, Chen W, Wu D, Qin Y, Liu M, Wu G, He L, Li H, Gu H Tags: Exp Cell Res Source Type: research
Long non-coding RNA-HAGLR suppressed tumor growth of lung adenocarcinoma through epigenetically silencing E2F1.
In this study, we investigated LncRNA alterations that contribute to lung cancer by analyzing published microarray data in Gene Expression Obminus (GEO) and The Cancer Genome Atlas RNA (TCGA) sequencing data. Here, we reported that HAGLR (also called HOXD-AS1) was frequently down-regulated in lung adenocarcinoma (LUAD) tissues, and decreased HAGLR expression was clinically associated with shorter survival of LUAD patients. Preclinical studies using multiple LUAD cells and in vivo mouse model indicated that HAGLR could attenuate LUAD cell growth in vitro and in vivo. Mechanistically, HAGLR could physically interact with DNM...
Source: Experimental Cell Research - June 10, 2019 Category: Cytology Authors: Guo X, Chen Z, Zhao L, Cheng D, Song W, Zhang X Tags: Exp Cell Res Source Type: research
Dipyridamole impairs autophagic flux and exerts antiproliferative activity on prostate cancer cells.
enz G Abstract Autophagy is a cellular bulk degradation process used as an alternative source of energy and metabolites and implicated in various diseases. Inefficient autophagy in nutrient-deprived cancer cells would be beneficial for cancer therapy making its modulation valuable as a therapeutic strategy for cancer treatment, especially in combination with chemotherapy. Dipyridamole (DIP) is a vasodilator and antithrombotic drug. Its major effects involve the block of nucleoside uptake and phosphodiestesase inhibition, leading to increased levels of intracellular cAMP. Here we report that DIP increases autophagi...
Source: Experimental Cell Research - June 10, 2019 Category: Cytology Authors: Thomé MP, Pereira LC, Onzi GR, Rohden F, Ilha M, Guma FT, Wink MR, Lenz G Tags: Exp Cell Res Source Type: research
Pharmacological inhibition of LIM kinase pathway impairs platelets functionality and facilitates thrombolysis.
Abstract Actin is highly abundant in platelets, and its function is dependent on its structure. Actin filaments (F-actin) are dynamic structures involved in many cellular processes including platelet shape changes and adhesion. The actin cytoskeleton is tightly regulated by actin-binding proteins, which include members of the actin depolymerising factor (ADF)/cofilin family. LIM kinase (LIMK) and its phosphatase slingshot (SSH-1L) regulate actin dynamics by controlling the binding affinity of ADF/cofilin towards actin. We hypothesised that the inhibition of LIMK activity may prevent the changes in platelet shape a...
Source: Experimental Cell Research - June 8, 2019 Category: Cytology Authors: Antonipillai J, Rigby S, Bassler N, Peter K, Bernard O Tags: Exp Cell Res Source Type: research
Fibroblast growth factor 21 ameliorates pancreatic fibrogenesis via regulating polarization of macrophages.
Abstract Chronic pancreatitis (CP) is a progressive, irreversible inflammatory and fibrotic disease. The characteristics of this disease are progressive inflammation, acinar atrophy and fibrosis. Numerous factors are involved in CP such as inflammation, and oxidative stress. Recently, it has been noted that fibroblast growth factor 21 (FGF-21) reduced the severity of acute pancreatitis in mice. However, whether FGF-21 has effects on CP remains unclear. Thus, the present study was undertaken to detect the effects of FGF-21 on l-arginine induced chronic pancreatitis/islet fibrosis in mice. We used l-arginine to crea...
Source: Experimental Cell Research - June 5, 2019 Category: Cytology Authors: Wang N, Zhao TT, Li SM, Li YH, Wang YJ, Li DS, Wang WF Tags: Exp Cell Res Source Type: research
Regulation of circadian rhythms by NEAT1 mediated TMAO-induced endothelial proliferation: A protective role of asparagus extract.
Abstract Trimethylamine N-oxide (TMAO) promotes atherosclerosis in association with the functions of endothelial cells. Clock and Bmal1, as two main components of molecular circadian clock, play important regulatory roles during progression of atherogenesis. However, whether Clock and Bmal1 are involved in the regulation of endothelial proliferation disturbed by TMAO are unclear. We observed that cell proliferation of human umbilical vein endothelial cells (HUVECs) was inhibited after exposed to TMAO for 24 h. Besides, TMAO caused increased expression of lncRNA-NEAT1, Clock and Bmal1, and inhibited MAPK pathways...
Source: Experimental Cell Research - June 4, 2019 Category: Cytology Authors: Wu X, Chen L, Zeb F, Huang Y, An J, Ren J, Yang F, Feng Q Tags: Exp Cell Res Source Type: research
Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3.
Abstract Mesenchymal stem cells (MSCs) have been widely documented for their potential role in the treatment of various clinical disorders, including acute lung injury (ALI). ALI represents a clinical syndrome associated with histopathological diffuse alveolar damage. Recent evidence has demonstrated that exosomes derived from MSCs may serve as a reservoir of anti-apoptotic microRNAs (miRs) conferring protection from certain diseases. Hence, the current study was performed with the aim of investigating whether MSCs-exosomal miR-30b-3p could confer protection against ALI. A bioinformatic analysis and a dual lucifer...
Source: Experimental Cell Research - June 3, 2019 Category: Cytology Authors: Yi X, Wei X, Lv H, An Y, Li L, Lu P, Yang Y, Zhang Q, Yi H, Chen G Tags: Exp Cell Res Source Type: research
Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.
Abstract Diabetic nephropathy (DN) is one of the most severe complications of diabetes mellitus. The pathomolecular events behind DN remain uncertain. Peroxisome proliferator-activated receptors (PPARs) play essential functions in the development of DN. Meanwhile, 20-hydroxyeicosatetraenoic acid (20-HETE) also plays central roles in the regulation of renal function. However, the relationship between PPARs and 20-HETE is rarely studied in DN. It was revealed in our study that both PPARs expression and CYP4A-20-HETE level were decreased under DN conditions in vivo and in vitro. Supplementation with bezafibrate, a PP...
Source: Experimental Cell Research - June 1, 2019 Category: Cytology Authors: Ding S, Huang J, Qiu H, Chen R, Zhang J, Huang B, Cheng O, Jiang Q Tags: Exp Cell Res Source Type: research
A novel long non-coding RNA TONSL-AS1 regulates progression of gastric cancer via activating TONSL.
In this study, we found a novel lncRNA, named TONSL-AS1, was downregulated in gastric cancer tissues and cell lines compared with the normal. TONSL-AS1 inhibited cell migration, invasion and proliferation in SGC-7901, MGC-803 cells. Furthermore, TONSL-AS1 could suppress cell tumorigenesis in vivo. Mechanistically, TONSL-AS1's genomic neighboring gene TONSL, which was reported as a tumor suppress gene, was upregulated by TONSL. Additionally, the TONSL-AS1 was positively associated with TONSL in cancer tissues. Our study revealed that the tumor-inhibiting effect of TONSL-AS1 in gastric cancer cells was associated with TONS...
Source: Experimental Cell Research - May 31, 2019 Category: Cytology Authors: Wang P, Yang X, Zhao L, Liu D, Liu J, Ding Y Tags: Exp Cell Res Source Type: research
Sox9 regulates the luminal stem/progenitor cell properties of salivary glands.
In this study, we purified the salivary gland stem/progenitor cells of adult mouse salivary glands using the cell surface marker CD133 by flow cytometry. CD133+ cells possessed stem cell capacity, and the transplantation of CD133+ cells into the submandibular gland reconstituted gland structures, including functional acinar. CD133+ cells were sparsely distributed in the intercalated and exocrine ducts and expressed Sox9 at higher levels than CD133- cells. Moreover, we demonstrated that Sox9 was required for the stem cell properties CD133+ cells, including colony and sphere formation. Thus, the Sox9-related signaling may co...
Source: Experimental Cell Research - May 30, 2019 Category: Cytology Authors: Tanaka J, Mabuchi Y, Hata K, Yasuhara R, Takamatsu K, Kujiraoka S, Yukimori A, Takakura I, Sumimoto H, Fukada T, Azuma M, Akiyama H, Nishimura R, Shimane T, Mishima K Tags: Exp Cell Res Source Type: research
Special issue on "Mechanotransduction in cell fate determination" - from molecular switches to organ-level regulation.
Special issue on "Mechanotransduction in cell fate determination" - from molecular switches to organ-level regulation. Exp Cell Res. 2019 May 30;: Authors: Wickström SA, Roca-Cusachs P PMID: 31153925 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 30, 2019 Category: Cytology Authors: Wickström SA, Roca-Cusachs P Tags: Exp Cell Res Source Type: research
Regulation of exosomes secretion by low-intensity pulsed ultrasound in lung cancer cells.
Abstract Low-intensity pulsed ultrasound (LIPUS) is a noninvasive therapeutic method which gradually being used in clinic including cancers. Exosomes mediate intercellular communication functions in disease development and the potential clinical applications in diagnosis and therapy. However, few studies have discussed the relationship between LIPUS and exosomes. Herein, we show that low intensity (0.6-2.1 W/cm2 or 0.6-3.4 W/cm2) LIPUS promoted exosomes secretion whereas higher intensity (3.4-5.0 W/cm2 or 5.0 W/cm2) LIPUS inhibited exosomes secretion, and this phenomenon is associated with autophagy. Pretr...
Source: Experimental Cell Research - May 29, 2019 Category: Cytology Authors: Zeng Q, Hong S, Wang X, Cheng Y, Sun J, Xia W Tags: Exp Cell Res Source Type: research
Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation.
Abstract MicroRNAs (miRNA) are small noncoding RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, thereby determining their degradation or inhibiting translation. They are involved in processes such as proliferation, differentiation and apoptosis by fine-tuning the expression of genes underlying such events. The expression of specific miRNAs is involved in hematopoietic differentiation and their deregulation contributes to the development of hematopoietic malignancies such as acute myeloid leukemia (AML). miR-130a is over-expressed in AML. Here we show that miR-130a is physiologic...
Source: Experimental Cell Research - May 29, 2019 Category: Cytology Authors: Mammoli F, Parenti S, Lomiento M, Gemelli C, Atene CG, Grande A, Corradini R, Manicardi A, Fantini S, Zanocco-Marani T, Ferrari S Tags: Exp Cell Res Source Type: research
MTA1 promotes the invasion and migration of oral squamous carcinoma by inducing epithelial-mesenchymal transition via hedgehog signaling pathway.
Abstract The metastasis-associated gene 1 (MTA1) has previously been recognized as an oncogene in many tumors, and aberrant MTA1 expression has been related to invasion and migration; however, its role and underlying molecular mechanism in oral squamous carcinoma (OSCC) remain largely unexplored. In this work, we determined the expression of MTA1 in OSCC tissues and cell lines. The effect of MTA1 on metastasis and the role of MTA1 in the epithelial-to-mesenchymal transition (EMT) of OSCC cells were evaluated by assays both in vitro and in vivo. We also identified the key Hedgehog signaling pathway-related protein ...
Source: Experimental Cell Research - May 29, 2019 Category: Cytology Authors: Song Q, Wang B, Liu M, Ren Z, Fu Y, Zhang P, Yang M Tags: Exp Cell Res Source Type: research
Heterotypic cell-in-cell structures in colon cancer can be regulated by IL-6 and lead to tumor immune escape.
Abstract Heterotypic CICs (cell-in-cell structures) have been found between tumor cells and various immune cells in a variety of cancer tissues. The frequency of CICs has been found to correlate with tumor malignancy in some studies but not in others. Herein, we examined in depth the CICs observed in colon cancer to determine their potential significance in disease progression. Heterotypic CICs were observed by histochemistry between epithelial cells and lymphocytes in an expanded spectrum of colon tissue from colitis to cancer and in vitro studies were performed using the colonic tumor cell line HCT8 and human pe...
Source: Experimental Cell Research - May 28, 2019 Category: Cytology Authors: Wang S, Li L, Zhou Y, He Y, Wei Y, Tao A Tags: Exp Cell Res Source Type: research
The morphological basis of the development of chick embryo immune system.
Abstract The chick immune system is a fundamental model in basic immunology. In birds, the bone marrow derived pluripotent stem cells after entering the circulation, migrate to bursa of Fabricius (BF) to benefit from a microenvironment which supports the differentiation and maturation of B lymphocytes by the help of its resident cells and tissues. Delivering sufficient functional B cells is required to maintain their peripheral population and normal peripheral humoral responses. Additionally, bursa acts as an active site for the generation of antibody diversity through gene conversion. Being consisted of 98% B lym...
Source: Experimental Cell Research - May 26, 2019 Category: Cytology Authors: Ribatti D, Tamma R, Ali Komi DE Tags: Exp Cell Res Source Type: research
The secretion of the angiogenic and neurotrophic factor angiogenin is COPII and microtubule dependent.
Abstract The RNaseA superfamily member Angiogenin (ANG) is a secreted protein involved in neovascularization, cell proliferation and stress response. Dysregulation of ANG expression is found in many cancers with poor prognosis and mutations in ANG are associated with neurodegenerative diseases. While the uptake and nuclear translocation of ANG is relatively well characterised, little is known about how it reaches the plasma membrane and its mode of secretion. We generated SH-SY5Y neuroblastoma cell lines constitutively expressing wild type (WT) Hemagglutinin (HA) epitope tagged mouse Ang1 (mAng1), and two amyotrop...
Source: Experimental Cell Research - May 22, 2019 Category: Cytology Authors: Ferguson R, Subramanian V Tags: Exp Cell Res Source Type: research
The role of the XBP-1/AGR2 signaling pathway in the regulation of airway Mucin5ac hypersecretion under hypoxia.
Abstract Oversecretion of Mucin5ac (MUC5AC), which is primarily synthesized by goblet cells and is the major gel-forming mucin, is a hallmark of various pulmonary inflammatory diseases. Hypoxia is considered a common pathophysiologic feature in various pulmonary inflammatory diseases. It has been suggested that hypoxia-inducible factor 1α (HIF-1α) acts as a key factor in hypoxia-induced MUC5AC hypersecretion; however, the exact mechanisms that maintain the stability of HIF-1α and support oversecretion by airway epithelial cells under hypoxia are still unclear. With immunohistochemistry, we found ...
Source: Experimental Cell Research - May 22, 2019 Category: Cytology Authors: Xu R, Zhou J, Du XZ, Zhou XD, Li Q, Perelman JM, Kolosov VP Tags: Exp Cell Res Source Type: research
Inhibition of glycogen catabolism induces intrinsic apoptosis and augments multikinase inhibitors in hepatocellular carcinoma cells.
Abstract Hepatocellular carcinoma (HCC) is one of the leading cancers in the world in incidence and mortality. Current pharmacotherapy of HCC is limited in the number and efficacy of anticancer agents. Metabolic reprogramming is a prominent feature of many cancers and has rekindled interest in targeting metabolic proteins for cancer therapy. Glycogen is a storage form of glucose, and the levels of glycogen have been found to correlate with biological processes in reprogrammed cancer cells. However, the contribution of glycogen metabolism to carcinogenesis, cancer cell growth, metastasis, and chemoresistance is poo...
Source: Experimental Cell Research - May 22, 2019 Category: Cytology Authors: Barot S, Abo-Ali EM, Zhou DL, Palaguachi C, Dukhande VV Tags: Exp Cell Res Source Type: research
TRB3 stimulates SIRT1 degradation and induces insulin resistance by lipotoxicity via COP1.
In this study, we explored the role of TRB3-COP1-SIRT1 in lipotoxicity leading to insulin resistance in hepatocytes. High fat diet (HFD)-fed mice and hepG2 cells stimulated with palmitate and were utilized as models of lipid metabolism disorders. We analyzed the interactions of SIRT1 and COP1 with each other and with TRB3 using co-immunoprecipitation, western blotting. SIRT1 ubiquitination was also explored. Animal and cell experiments showed that lipotoxicity induced SIRT1 down-regulation at the protein level without altering the mRNA level, whereas, lipotoxicity led to up-regulation of TRB3 and COP1 at both the gene and ...
Source: Experimental Cell Research - May 21, 2019 Category: Cytology Authors: Ren X, Chen N, Chen Y, Liu W, Hu Y Tags: Exp Cell Res Source Type: research
A novel specific cleavage of I κBα protein in acute myeloid leukemia cells involves protease PR3.
A novel specific cleavage of IκBα protein in acute myeloid leukemia cells involves protease PR3. Exp Cell Res. 2019 May 21;: Authors: Wang MM, Zhuang LK, Zhang YT, Xia D, Pan XR, Tong JH Abstract IκBα protein plays an important role in NFκB signaling pathway regulation. The dysfunction of IκBα is tightly related to various diseases, including cancers. However, the molecular mechanisms by which IκBα loses its normal functions are diverse and complex. Here, we reported a novel cleavage of IκBα protein occurred in AML cells. Compared with ...
Source: Experimental Cell Research - May 21, 2019 Category: Cytology Authors: Wang MM, Zhuang LK, Zhang YT, Xia D, Pan XR, Tong JH Tags: Exp Cell Res Source Type: research
The E6/E7 oncogenes of human papilloma virus and estradiol regulate hedgehog signaling activity in a murine model of cervical cancer.
C, Covarrubias L Abstract Human papilloma virus oncogenes and estradiol are major etiologic factors associated with cervical cancer. In order to understand the mechanism by which these two factors promote carcinogenesis, the role of the Hedgehog (Hh) signaling pathway was evaluated during the normal growth of cervical epithelium and in the presence of E6/E7 oncogenes and exogenous estradiol. Hh signaling activity was determined in live animals (i.e., Gli-Luc reporter levels) during the estrous cycle and was found to be higher in the cervical area during the major growth phases, proestrus-estrus, in comparison to ...
Source: Experimental Cell Research - May 21, 2019 Category: Cytology Authors: Rojo-León V, García C, Valencia C, Méndez MA, Wood C, Covarrubias L Tags: Exp Cell Res Source Type: research
The paracrine effects of fibroblasts on doxorubicin-treated breast cancer cells.
Abstract Breast cancer is frequently diagnosed in women and poses a major health problem throughout the world. Currently, the unresponsiveness of cancer cells to chemotherapeutics is a major concern. During chemotherapeutic treatment with Doxorubicin, neighbouring cells in the tumor microenvironment are also damaged. Depending on the concentration of Doxorubicin, apoptotic or senescent fibroblasts in the tumor microenvironment can then secrete a variety of bioactive molecules which promote tumor growth, metastasis and drug resistance. Mouse embryonic fibroblasts (MEFs) were treated with Doxorubicin to induce apopt...
Source: Experimental Cell Research - May 20, 2019 Category: Cytology Authors: Fourie C, Davis T, Kriel J, Engelbrecht AM Tags: Exp Cell Res Source Type: research
Fam208a orchestrates interaction protein network essential for early embryonic development and cell division.
Abstract Maintenance of genome stability is essential for every living cell as genetic information is repeatedly challenged during DNA replication in each cell division event. Errors, defects, delays, and mistakes that arise during mitosis or meiosis lead to an activation of DNA repair processes and in case of their failure, programmed cell death, i.e. apoptosis, could be initiated. Fam208a is a protein whose importance in heterochromatin maintenance has been described recently. In this work, we describe the crucial role of Fam208a in sustaining the genome stability during the cellular division. The targeted deple...
Source: Experimental Cell Research - May 18, 2019 Category: Cytology Authors: Gresakova V, Novosadova V, Prochazkova M, Bhargava S, Jenickova I, Prochazka J, Sedlacek R Tags: Exp Cell Res Source Type: research
Inhibition of degranulation in mast cells attached to a hydrogel through defective microtubule tracts.
Abstract Mast cells (MCs) are important effectors of the immediate allergic response. MCs are distributed throughout various tissues and organs, and adhere to extracellular matrix (ECM) with broad stiffness in the body. Here we compared cellular responses following antigen stimulation in MCs on glass-base dishes with and without a hydrogel. We found that an antigen-induced increase in intracellular Ca2+ concentration was suppressed slightly in cells on hydrogel-coated dishes compared with those on non-coated dishes, whereas their subsequent degranulation was largely inhibited in cells adherent to the hydrogel. Foc...
Source: Experimental Cell Research - May 18, 2019 Category: Cytology Authors: Shiki A, Inoh Y, Yokawa S, Furuno T Tags: Exp Cell Res Source Type: research
Pivotal role of mitophagy in response of acute myelogenous leukemia to a ceramide-tamoxifen-containing drug regimen.
Abstract Acute myelogenous leukemia (AML) is a hematological malignancy marked by the accumulation of large numbers of immature myeloblasts in bone marrow. The overall prognosis in AML is poor; hence, there is a pressing need to improve treatment. Although the sphingolipid (SL) ceramide demonstrates known cancer suppressor properties, it's mechanism of action is multifaceted. Our studies in leukemia and other cancers have demonstrated that when combined with the antiestrogen, tamoxifen, the apoptosis-inducting effect of ceramide is greatly enhanced. The goal of the present study was to establish whether a ceramide...
Source: Experimental Cell Research - May 18, 2019 Category: Cytology Authors: Morad SAF, MacDougall MR, Abdelmageed N, Kao LP, Feith DJ, Tan SF, Kester M, Loughran TP, Wang HG, Cabot MC Tags: Exp Cell Res Source Type: research
The human Bcl-2 family member Bcl-rambo and voltage-dependent anion channels manifest a genetic interaction in Drosophila and cooperatively promote the activation of effector caspases in human cultured cells.
Abstract We previously reported that the Bcl-2 family member human Bcl-rambo, also known as BCL2L13, induces apoptosis in human embryonic kidney 293T cells. Mouse Bcl-rambo has recently been reported to mediate mitochondrial fragmentation and mitophagy. In the present study, we showed that the transfection of human Bcl-rambo and its microtubule-associated protein light chain 3-interacting region motif mutant (W276A/I279A) caused mitochondrial fragmentation and the perinuclear accumulation of fragmented mitochondria in human lung adenocarcinoma A549 cells. In comprehensive screening using the Drosophila model in ...
Source: Experimental Cell Research - May 15, 2019 Category: Cytology Authors: Matsubara H, Tanaka R, Tateishi T, Yoshida H, Yamaguchi M, Kataoka T Tags: Exp Cell Res Source Type: research
Destination and consequences of Panx1 and mutant expression in polarized MDCK cells.
Abstract The channel-forming membrane glycoprotein pannexin 1 (Panx1) is best characterized as an ATP release channel. To investigate the trafficking and sorting of Panx1, we used polarized MDCK cells and non-polarized BICR-M1Rk cells to track the fate of GFP-tagged Panx1. In non-polarized cells, Panx1 was found throughout the plasma membrane, including the lamellipodia of the tumor cells and the cell surface-targeting domain was mapped to residues 307-379. Panx1 was preferentially enriched at the apical membrane domain of polarized MDCK cells grown as monolayer sheets or as spheroids. Residual Panx1 localized wit...
Source: Experimental Cell Research - May 15, 2019 Category: Cytology Authors: Shum MG, Shao Q, Lajoie P, Laird DW Tags: Exp Cell Res Source Type: research
Ligand-activated progesterone receptor B activates transcription factor EB to promote autophagy in human breast cancer cells MCF-7.
Abstract Autophagy is an evolutionary conserved, self-eating process that targets cellular constituents for lysosomal degradation. Transcription factor EB (TFEB) is a master regulator of autophagy by inducing the expression of genes involved in autophagic and lysosomal degradation. In breast cancer, ligand-activated progesterone receptor has been reported to influence cancer development by manipulating the autophagy pathway. However, understanding of the mechanism that underlies the autophagic response remains limited. Herein, we report that prolonged treatment with progestin R5020 upregulates autophagy in MCF-7 h...
Source: Experimental Cell Research - May 14, 2019 Category: Cytology Authors: Tan S, Natasa B, Wong ESP, Lin VCL Tags: Exp Cell Res Source Type: research
Interleukin 6-triggered ataxia-telangiectasia mutated kinase activation facilitates epithelial-to-mesenchymal transition in lung cancer by upregulating vimentin expression.
Abstract Matrix metalloproteinases (MMPs) and the epithelial-mesenchymal transition (EMT) contribute to metastasis. As shown in our previous studies, interleukin-6 (IL-6) induces ATM phosphorylation to increase MMP expression and metastasis in lung cancer. However, the exact roles of ATM activation in the IL-6-induced epithelial-mesenchymal transition and lung cancer metastasis are currently unclear. Here, ATM phosphorylation exerts its pro-metastatic effect via vimentin-mediated epithelial-mesenchymal transition, which was supported by the evidence described below. Firstly, IL-6 treatment increases vimentin expre...
Source: Experimental Cell Research - May 14, 2019 Category: Cytology Authors: Jiang YN, Ni XY, Yan HQ, Shi L, Lu N, Wang YN, Li Q, Gao FG Tags: Exp Cell Res Source Type: research
PAQR3 regulates phosphorylation of FoxO1 in insulin-resistant HepG2 cells via NF- κB signaling pathway.
In this study, we aimed to investigate whether PAQR3 regulates phosphorylation of FoxO1 via NF-κB pathway in palmitic acid (PA)-induced insulin-resistant HepG2 cells, thereby causing glucose and lipid metabolism disorders. We found that PA stimulation and PAQR3 overexpression decreased the phosphorylation of FoxO1 and the expressions of glucokinase (GCK) and low density lipoprotein receptor (LDLR), in addition, promoted the nuclear accumulation of NF-κB. Inhibition of NF-κB pathway increased the phosphorylation of FoxO1 and the expressions of GCK and LDLR which were downregulated by PA stimulation and PAQ...
Source: Experimental Cell Research - May 13, 2019 Category: Cytology Authors: Chen L, Sun X, Xiao H, Xu F, Yang Y, Lin Z, Chen Z, Quan S, Huang H Tags: Exp Cell Res Source Type: research
LncRNA-H19 regulates cell proliferation and invasion of ectopic endometrium by targeting ITGB3 via modulating miR-124-3p.
Abstract Endometriosis, a common gynecological disease, is associated with pelvic pain and infertility. Endometriosis affects approximately 10% of women, but that number increases to 30-50% in symptomatic premenopausal women. Despite the prevalence of endometriosis, the cause has yet to be fully elucidated. Recent study of the molecular pathways of endometrial cancer has brought the long non-coding RNA (lncRNA) H19 to our attention. In this paper, we explored the role of lncRNA-H19 in endometrial tissue proliferation. We found that ectopic endometrial cells taken from women with endometriosis showed elevated level...
Source: Experimental Cell Research - May 11, 2019 Category: Cytology Authors: Liu S, Qiu J, Tang X, Cui H, Zhang Q, Yang Q Tags: Exp Cell Res Source Type: research
Guanine-rich RNA binding protein GRSF1 inhibits myoblast differentiation through repressing mitochondrial ROS production.
Abstract Guanine-rich RNA sequence binding factor 1 (GRSF1) is a member of the RNA-binding protein (RBP) family. GRSF1 regulates RNA metabolism through RNA processing, transport and translation in the cytoplasm and mitochondria. However, its role in myogenesis has not been investigated. Here, we demonstrated that the expression of mitochondrial GRSF1 was negatively related to the differentiation of mouse skeletal myoblasts. Interference with GRSF1 promotes myogenesis both in vitro and in vivo without affecting MyoD expression or cell proliferation. Further studies illustrated that GRSF1 regulated myogenic differen...
Source: Experimental Cell Research - May 11, 2019 Category: Cytology Authors: Yin W, Yang L, Kong D, Nie Y, Liang Y, Teng CB Tags: Exp Cell Res Source Type: research
A "noisy" electrical stimulation protocol favors muscle regeneration in vitro through release of endogenous ATP.
In conclusion, our results show that "noisy" electrical stimulations favor muscle progenitor cell differentiation most likely via the release of endogenous ATP from contracting myofibres. Our data also suggest that "noisy" stimulation protocols could be potentially more efficient than regular stimulations to promote in vivo muscle regeneration after traumatic injury or in neuropathological diseases. PMID: 31082374 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 10, 2019 Category: Cytology Authors: Bosutti A, Bernareggi A, Massaria G, D'Andrea P, Taccola G, Lorenzon P, Sciancalepore M Tags: Exp Cell Res Source Type: research
Altered actin cytoskeleton in ageing eggs of starfish affects fertilization process.
Abstract Integrity of oocytes is of pivotal interest in the medical and zootechnical practice of in vitro fertilization. With time, oocytes undergo deterioration in quality, and ageing oocytes often exhibit compromised competence in fertilization and the subsequent embryonic development. With ageing oocytes and eggs of starfish (Astropecten aranciacus), we addressed the issue by examining changes of the subcellular structure and their performance at fertilization. Ageing eggs were simulated in two different experimental paradigms: i) oocytes were overmatured by 6 h stimulation with 1-methyladenine (1-MA); ii) oo...
Source: Experimental Cell Research - May 10, 2019 Category: Cytology Authors: Limatola N, Vasilev F, Chun JT, Santella L Tags: Exp Cell Res Source Type: research
Corrigendum to "The PDZ-GEF Gef26 regulates synapse development and function via FasII and Rap 1 at the Drosophila neuromuscular junction" [Exp. Cell Res. 374 (2019) 342-352].
Corrigendum to "The PDZ-GEF Gef26 regulates synapse development and function via FasII and Rap 1 at the Drosophila neuromuscular junction" [Exp. Cell Res. 374 (2019) 342-352]. Exp Cell Res. 2019 May 10;: Authors: Ou M, Wang S, Sun M, An J, Lv H, Zeng X, Hou SX, Xie W PMID: 31085014 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 10, 2019 Category: Cytology Authors: Ou M, Wang S, Sun M, An J, Lv H, Zeng X, Hou SX, Xie W Tags: Exp Cell Res Source Type: research
Corrigendum to "Metabolic switching in pluripotent stem cells reorganizes energy metabolism and subcellular organelles" [379 (2019) 55-64].
Corrigendum to "Metabolic switching in pluripotent stem cells reorganizes energy metabolism and subcellular organelles" [379 (2019) 55-64]. Exp Cell Res. 2019 May 09;: Authors: O'Reilly C, Cho JH, Qi Q, Peters JL, Fukuda Y, Frase S, Peng J, Schuetz JD, Cheng Y, Yoon SO, Han MJ PMID: 31079852 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 9, 2019 Category: Cytology Authors: O'Reilly C, Cho JH, Qi Q, Peters JL, Fukuda Y, Frase S, Peng J, Schuetz JD, Cheng Y, Yoon SO, Han MJ Tags: Exp Cell Res Source Type: research
Inhibition of protein nitration prevents cisplatin-induced inactivation of STAT3 and promotes anti-apoptotic signaling in organ of Corti cells.
Abstract JAK/STAT pathway is one among the several oxidative stress-responsive signaling pathways that play a critical role in facilitating cisplatin-induced ototoxicity. Cisplatin treatment decreases the levels of cochlear LMO4, which acts as a scaffold for IL6-GP130 protein complex. Cisplatin-induced nitration and degradation of LMO4 could destabilize this protein complex, which in turn could compromise the downstream STAT3-mediated cellular defense mechanism. Here, we investigated the link between cisplatin-induced nitrative stress and STAT3-mediated apoptosis by using organ of Corti cell cultures. SRI110, a pe...
Source: Experimental Cell Research - May 9, 2019 Category: Cytology Authors: Rosati R, Shahab M, Neumann WL, Jamesdaniel S Tags: Exp Cell Res Source Type: research
Interaction of Sox2 with RNA binding proteins in mouse embryonic stem cells.
In this study, we determined the protein interactome of Sox2 in the chromatin and nucleoplasm of mouse embryonic stem (mES) cells. Apart from canonical interactions with pluripotency-regulating transcription factors, we identified interactions with several chromatin modulators, including members of the heterochromatin protein 1 (HP1) family, suggesting a role of Sox2 in chromatin-mediated transcriptional repression. Sox2 was also found to interact with RNA binding proteins (RBPs), including proteins involved in RNA processing. RNA immunoprecipitation followed by sequencing revealed that Sox2 associates with different messe...
Source: Experimental Cell Research - May 8, 2019 Category: Cytology Authors: Samudyata, Amaral PP, Engström PG, Robson SC, Nielsen ML, Kouzarides T, Castelo-Branco G Tags: Exp Cell Res Source Type: research
Collagen triple helix repeat containing 1a (Cthrc1a) regulates cell adhesion and migration during gastrulation in zebrafish.
Abstract Cell adhesion and migration are key cell behaviours during gastrulation in early embryos and metastasis in cancers. Cthrc1 is a secreted protein highly conserved among vertebrates; it is upregulated in injured and diseased arteries, as well as in malignant cancers. There is increasing evidence showing that its expression and activity are associated with cancer progression and inflammatory diseases. However, the mechanism by which it regulates cell migration, and its implication during early development remains unclear. Here we show that zebrafish Cthrc1a is expressed in hypoblast cells, and is required fo...
Source: Experimental Cell Research - May 8, 2019 Category: Cytology Authors: Cheng XN, Shao M, Shi DL Tags: Exp Cell Res Source Type: research
Compressive stress-induced autophagy promotes invasion of HeLa cells by facilitating protein turnover in vitro.
In this study, we have investigated whether compressive force induces autophagy in HeLa cells with potential implications in cellular invasiveness. To this end, we have adopted a simple strategy to create the mechanically-compressed tumor microenvironment, in vitro, by applying appropriate compression to agarose-scaffolded HeLa cell-encapsulated alginate beads. Our findings confirm that compression upregulates autophagy, which promotes paxillin turnover and active MMP 2 secretion, leading to enhanced migration of HeLa cells. We further show that autophagy induction by compression is affected by the phosphorylation of p38MA...
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Das J, Agarwal T, Chakraborty S, Maiti TK Tags: Exp Cell Res Source Type: research
Recombinant Treponema pallidum protein Tp47 promotes the migration and adherence of THP-1 cells to human dermal vascular smooth muscle cells by inducing MCP-1 and ICAM-1 expression.
This study provides a novel potential therapeutic strategy for controlling the vascular inflammatory response in syphilis patients. PMID: 31075255 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Gao ZX, Liu D, Liu LL, Lin LR, Tong ML, Niu JJ, Yang TC Tags: Exp Cell Res Source Type: research
Targeting the unfolded protein response in head and neck and oral cavity cancers.
Abstract Many FDA-approved anti-cancer therapies, targeted toward a wide array of molecular targets and signaling networks, have been demonstrated to activate the unfolded protein response (UPR). Despite a critical role for UPR signaling in the apoptotic execution of cancer cells by many of these compounds, the authors are currently unaware of any instance whereby a cancer drug was developed with the UPR as the intended target. With the essential role of the UPR as a driving force in the genesis and maintenance of the malignant phenotype, a great number of pre-clinical studies have surged into the medical literatu...
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Cole DW, Svider PF, Shenouda KG, Lee PB, Yoo N, McLeod TM, Mutchnick S, Yoo GH, Kaufman RJ, Callaghan MU, Fribley AM Tags: Exp Cell Res Source Type: research
Quantitative assessment of changes in cell growth, size and morphology during telomere-initiated cellular senescence in Saccharomyces cerevisiae.
In this study, a new assay is described that allowed quantitation of senescence in telomerase-deficient est2 cells with applied statistics. Use of the new technique revealed that senescence was strongly accelerated in est2 mutants that had homologous recombination genes RAD51, RAD52 or RAD54 co-inactivated, but was only modestly affected when RAD55, RAD57 or RAD59 were knocked out. Additionally, a new approach for calculating population doublings indicated that loss of growth capacity occurred after approximately 64 generations in est2 cells but only 42 generations in est2 rad52 cells. Phase contrast microscopy experimen...
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Ghanem NZ, Malla SRL, Araki N, Lewis LK Tags: Exp Cell Res Source Type: research
Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation.
Abstract Eph receptor and ephrin signaling has a major role in segregating distinct cell populations to form sharp borders. Expression of interacting Ephs and ephrins typically occurs in complementary regions, such that polarised activation of both components occurs at the interface. Forward signaling through Eph receptors can drive cell segregation, but it is unclear whether reverse signaling through ephrins can also contribute. We have tested the role of reverse signaling, and of polarised versus non-polarised activation, in assays in which contact repulsion drives cell segregation and border sharpening. We find...
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Wu Z, Ashlin TG, Xu Q, Wilkinson DG Tags: Exp Cell Res Source Type: research
Glucose impairs angiogenesis and promotes ventricular remodelling following myocardial infarction via upregulation of microRNA-17.
Abstract Hyperglycaemia is known to impair angiogenesis, which may contribute to the poor prognosis of diabetic patients following myocardial infarction (MI). miR-17 has been reported to be involved in the proliferation, migration, and angiogenesis of a variety of vascular endothelial cells. However, how miR-17 regulates angiogenesis under hyperglycaemic conditions has not been reported. Thus, the aim of this study was to investigate the role of miR-17 in the impairment of angiogenesis induced by high glucose. In vitro, human umbilical vein endothelial cells (HUVECs) transfected with miR-17 mimics or inhibitors we...
Source: Experimental Cell Research - May 7, 2019 Category: Cytology Authors: Yan M, Chen K, Sun R, Lin K, Qian X, Yuan M, Wang Y, Ma J, Qing Y, Xu J, Wei M, Huang D, Li J Tags: Exp Cell Res Source Type: research