BST-2/Tetherin is involved in BAFF-enhanced proliferation and survival via canonical NF- κB signaling in neoplastic B-lymphoid cells.
In this study, we identified that BST-2 can respond to BAFF simulation but not to other B cell simulators in neoplastic B cell lines. A CCK-8 assay, an EdU assay and Annexin V/PI staining indicated that BST-2 inhibition attenuated BAFF-enhanced proliferation and survival in both Raji cells and Daudi cells. Screening of BAFF-related signaling in neoplastic B-lymphoid cells indicated that BST-2 was involved in the regulation of NF-κB signaling upon BAFF simulation. However, inhibition of NF-κB by JSH-23 significantly reduced the proliferation and survival of Raji and Daudi cells under both normal and BAFF-simulat...
Source: Experimental Cell Research - November 24, 2020 Category: Cytology Authors: Fu J, Shi H, Zhan T, Li H, Ye L, Xie L, Wang Z, Wang B, Zheng L Tags: Exp Cell Res Source Type: research

Pristane promotes anaerobic glycolysis to facilitate proinflammatory activation of macrophages and development of arthritis.
Abstract Pristane-induced arthritis (PIA) could be adoptively transferred by splenic T cells in rats, and innate immunity should play critical roles in T cell activation. However, in pre-clinical stage, the activation mechanism of innate cells like macrophages remains unclear. Here we found that PIA was dependent on macrophages since cell depletion alleviated disease severity. Splenic macrophages of PIA rats showed M1 phenotypic shifting. The quantitative proteomics analysis suggested that macrophages initiated metabolic reprogramming with the conversion of aerobic oxidation to glycolysis in response to pristane i...
Source: Experimental Cell Research - November 24, 2020 Category: Cytology Authors: Li X, Gao F, Zhu W, Jiang C, Xu J, Zhang J, Meng L, Lu S Tags: Exp Cell Res Source Type: research

HIF1 α promotes tumor chemoresistance via recruiting GDF15-producing TAMs in colorectal cancer.
HIF1α promotes tumor chemoresistance via recruiting GDF15-producing TAMs in colorectal cancer. Exp Cell Res. 2020 Nov 23;:112394 Authors: Zheng H, Yu S, Zhu C, Guo T, Liu F, Xu Y Abstract Chemoresistance is a tremendous challenge to efficacy of systemic chemotherapy which is the preferred treatment for the advanced CRC patients. More tumor-associated macrophages (TAMs) are recruited into the CRC tumor under chemotherapy, which are highly implicated in the chemoresistance development, but the underlying molecular mechanism is unclear. Here, we present that activated HIF1α signaling in CRC c...
Source: Experimental Cell Research - November 23, 2020 Category: Cytology Authors: Zheng H, Yu S, Zhu C, Guo T, Liu F, Xu Y Tags: Exp Cell Res Source Type: research

Combination therapy with lenvatinib and radiation significantly inhibits thyroid cancer growth by uptake of tyrosine kinase inhibitor.
In this study, we investigated the antitumor effects of combination therapy with lenvatinib and radiation (CTLR) for DTC. CTLR synergistically inhibited cell replication and colony formation in vitro and tumor growth in nude mice without apparent toxicities and suppressed the expression of proliferation marker (Ki-67). CTLR also induced apoptosis and G2/M phase cell cycle arrest. Moreover, quantitative analysis of the intracellular uptake of lenvatinib using liquid chromatography and mass spectrometry demonstrated that intracellular uptake of lenvatinib was significantly increased 48 h following irradiation. These data s...
Source: Experimental Cell Research - November 20, 2020 Category: Cytology Authors: Suzuki K, Iwai H, Utsunomiya K, Kono Y, Kobayashi Y, Van Bui D, Sawada S, Yun Y, Mitani A, Kondo N, Katano T, Tanigawa N, Akama T, Kanda A Tags: Exp Cell Res Source Type: research

Plasminogen activator Inhibitor-2 inhibits pulmonary arterial smooth muscle cell proliferation in pulmonary arterial hypertension via PI3K/Akt and ERK signaling.
CONCLUSION: PAI-2 is downregulated in PAH patients. PAI-2 attenuates PAH development by suppressing hPASMCs proliferation via the inhibition of PI3K/Akt and ERK pathways. PAI-2 may serve as a potential biomarker and therapeutic target for PAH. PMID: 33227315 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 20, 2020 Category: Cytology Authors: Zhang S, Wang J, Qi X, Tao X, Xie W, Wan J, Shen YH, Zhai Z Tags: Exp Cell Res Source Type: research

Retraction Notice to "Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells" [Exp. Cell Res. 333 (2015) 303-315].
Retraction Notice to "Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells" [Exp. Cell Res. 333 (2015) 303-315]. Exp Cell Res. 2020 Nov 19;:112363 Authors: Ozeki N, Hase N, Yamaguchi H, Hiyama T, Kawai R, Kondo A, Nakata K, Mogi M PMID: 33223132 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Ozeki N, Hase N, Yamaguchi H, Hiyama T, Kawai R, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

FHOD formin and SRF promote post-embryonic striated muscle growth through separate pathways in C. elegans.
Abstract Previous work with cultured cells has shown transcription of muscle genes by serum response factor (SRF) can be stimulated by actin polymerization driven by proteins of the formin family. However, it is not clear if endogenous formins similarly promote SRF-dependent transcription during muscle development in vivo. We tested whether formin activity promotes SRF-dependent transcription in striated muscle in the simple animal model, Caenorhabditis elegans. Our lab has shown FHOD-1 is the only formin that directly promotes sarcomere formation in the worm's striated muscle. We show here FHOD-1 and SRF homolog ...
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Yingling CV, Pruyne D Tags: Exp Cell Res Source Type: research

A novel in vivo mouse intervertebral disc degeneration model induced by compressive suture.
Abstract Intervertebral disc degeneration (IDD) is the root cause of many musculoskeletal disorders of the spine. However, the etiology of IDD is complex and still not well understood. Animal models of IDD would be useful in deciphering the underlying mechanisms. But the existing animal models have their limitations. Therefore, to establish a novel mouse model that can simulate the human IDD process in vivo, we proposed to carefully circumcise the 2 mm-wide tail skin and then compressively sutured the defect with a simple end-to-end suture to exert excessive pressure on the disc. After 1-week, 2-week, and 4-week...
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Liu Z, Zhou Q, Zheng J, Li C, Zhang W, Zhang X Tags: Exp Cell Res Source Type: research

Activation of the CaR-CSE/H2S pathway confers cardioprotection against ischemia-reperfusion injury.
Abstract Ischemia-reperfusion (I/R) injury is a multifactorial process triggered when an organ is subjected to transiently reduced blood supply. The result is a cascade of pathological complications and organ damage due to the production of reactive oxygen species following reperfusion. The present study aims to evaluate the role of activated calcium-sensing receptor (CaR)-cystathionine γ-lyase (CSE)/hydrogen sulfide (H2S) pathway in I/R injury. Firstly, an I/R rat model with CSE knockout was constructed. Transthoracic echocardiography, TTC and HE staining were performed to determine the cardiac function of ...
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Luo Y, Liu LM, Xie L, Zhao HL, Lu YK, Wu BQ, Wu ZY, Zhang ZL, Hao YL, Ou WH, Liu RS, Xu WM, Chen XH Tags: Exp Cell Res Source Type: research

Extracellular vesicles promote epithelial-to-mesenchymal transition of lens epithelial cells under oxidative stress.
In this study, we examined the secreted EVs from a scratch model in vitro. We found that the production of ROS was increased after mechanical injury, especially at the wound edge, and there was an increased viability of LECs, which can be blocked by diphenyleneiodonium, an NADPH oxidase inhibitor. Cell viability and migration were increased upon treatment with 1 μM H2O2, but significantly reduced when the concentration of H2O2 increased to 100 μM. Transwell assay showed that both post-surgery LECs and LECs treated with 1 μM H2O2 significantly induced the migration of normal LECs by EV secretion. Extracti...
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Wang R, Li J, Zhang X, Zhang X, Zhang X, Zhu Y, Chen C, Liu Z, Wu X, Wang D, Dongye M, Wang J, Lin H Tags: Exp Cell Res Source Type: research

NMIIA promotes tumorigenesis and prevents chemosensitivity in colorectal cancer by activating AMPK/mTOR pathway.
In this study, we found that NMIIA was overexpressed in CRC tissues and significantly associated with poor survival in CRC patients. In addition, NMIIA promoted CRC cell proliferation and invasion via activating the AMPK/mTOR pathway in vitro, and NMIIA knockdown inhibited CRC growth in vivo. Meanwhile, NMIIA knockdown downregulated the CSCs markers (CD44 and CD133) expression in CRC cells. Furthermore, AMPK/mTOR pathway activation effectively reversed the NMIIA knockdown-induced inhibition of proliferation, invasion and stemness in CRC cells. Finally, NMIIA protects CRC cells from 5-FU-induced apoptosis and proliferation ...
Source: Experimental Cell Research - November 18, 2020 Category: Cytology Authors: Wang Z, Zhu Z, Li C, Zhang Y, Li Z, Sun S Tags: Exp Cell Res Source Type: research

PGC1 α regulates mitochondrial oxidative phosphorylation involved in cisplatin resistance in ovarian cells via nucleo-mitochondrial transcriptional feedback.
PGC1α regulates mitochondrial oxidative phosphorylation involved in cisplatin resistance in ovarian cells via nucleo-mitochondrial transcriptional feedback. Exp Cell Res. 2020 Nov 18;:112369 Authors: Shen L, Zhou L, Xia M, Lin N, Ma J, Dong D, Sun L Abstract Mitochondria play an important role in effective cell energy production and cell survival under stress conditions, such as treatment with chemotherapeutic drugs. Mitochondrial biogenesis is increased in ovarian cancer tissues, which is accompanied by alteration of mitochondrial energy metabolism, structure, and dynamics. These factors are in...
Source: Experimental Cell Research - November 18, 2020 Category: Cytology Authors: Shen L, Zhou L, Xia M, Lin N, Ma J, Dong D, Sun L Tags: Exp Cell Res Source Type: research

Cold shock induces novel nuclear bodies in Xenopus oocytes.
Abstract Here we describe novel spherical structures that are induced by cold shock on the lampbrush chromosomes (LBCs) of Xenopus laevis oocytes. We call these structures cold bodies or C-bodies. C-bodies are distributed symmetrically on homologous LBCs, with a pattern similar to that of 5S rDNA. Neither active transcription nor translation is necessary for their formation. Similar protrusions occur on the edges of some nucleoli. Endogenous LBCs as well as those derived from injected sperm form C-bodies under cold shock conditions. The function of C-bodies is unknown. PMID: 33220259 [PubMed - as supplied by ...
Source: Experimental Cell Research - November 18, 2020 Category: Cytology Authors: Liu JL, Gall JG Tags: Exp Cell Res Source Type: research

Absence of the Shb gene in mixed-lineage leukemia MLL-AF9 cells increases latency in mice despite higher proliferation rates in vitro.
Abstract Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and Shb knockout (KO) mice were transduced with the MLL-AF9 gene. Shb KO MLL-AF9 cells proliferated at an increased rate, exhibited altered expression of certain cytokine genes (Kitl, Csf3, IL6, IL1b) and higher expression of cell cycle genes (Ccnd2, Ccne1). Mice receiving Shb KO MLL-AF9 cells sho...
Source: Experimental Cell Research - November 18, 2020 Category: Cytology Authors: Jamalpour M, Bergquist E, Welsh M Tags: Exp Cell Res Source Type: research

Ubiquitin-specific protease 14 promotes radio-resistance and suppresses autophagy in oral squamous cell carcinoma.
In this study, we identified a mechanism of such resistance regulated by Ubiquitin-specific protease 14 (USP14). USP14 expression was significantly increased in clinical OSCC tissue samples and cell lines, and OSCC patients with high USP14 expression predicted poor overall survival rate. Additionally, a negative correlation between USP14 and LC3B was observed in patients with OSCC. We then found that irradiation (IR)-reduced cell survival of OSCC cells lines was further decreased when USP14 was knocked down. However, USP14 over-expression significantly promoted the cell viability of OSCC cells after IR treatment. Colony fo...
Source: Experimental Cell Research - November 16, 2020 Category: Cytology Authors: Xie W, Xu L Tags: Exp Cell Res Source Type: research

Long noncoding RNA Nespas inhibits apoptosis of epileptiform hippocampal neurons by inhibiting the PI3K/Akt/mTOR pathway.
This report explored the function and regulatory mechanism of Nespas in epileptiform hippocampal neurons for the first time. Our findings revealed that Nespas suppressed the apoptosis of epileptiform hippocampal neurons by inhibiting the PI3K/Akt/mTOR pathway via upregulation of Psmd11 at a miR-615-3p dependent way, indicating that Nespas may offer a new direction for the treatment of epilepsy. PMID: 33212147 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 16, 2020 Category: Cytology Authors: Feng H, Gui Q, Wu G, Zhu W, Dong X, Shen M, Fu X, Shi G, Luo H, Yang X, Xue S, Cheng Q Tags: Exp Cell Res Source Type: research

Winter is coming: Regulation of cellular metabolism by enzyme polymerization in dormancy and disease.
arikangas J Abstract Metabolism feeds growth. Accordingly, metabolism is regulated by nutrient-sensing pathways that converge growth promoting signals into biosynthesis by regulating the activity of metabolic enzymes. When the environment does not support growth, organisms invest in survival. For cells, this entails transitioning into a dormant, quiescent state (G0). In dormancy, the activity of biosynthetic pathways is dampened, and catabolic metabolism and stress tolerance pathways are activated. Recent work in yeast has demonstrated that dormancy is associated with alterations in the physicochemical properties ...
Source: Experimental Cell Research - November 16, 2020 Category: Cytology Authors: Montrose K, López Cabezas RM, Paukštytė J, Saarikangas J Tags: Exp Cell Res Source Type: research

Expression and role of MIG/CXCR3 axis in mantle cell lymphoma.
Abstract Mantle cell lymphoma (MCL) is a unique subtype of B-cell non-Hodgkin lymphoma with a generally aggressive and heterogeneous clinical course. Chemokines are one of the complex components in the tumor microenvironment (TME), and they play a vital role in tumor progression and metastasis. There is no information about the monokine induced by gamma interferon (MIG)/CXC chemokine receptor 3 (CXCR3) axis in patients with MCL. In the present study, we discovered that CXCR3 was highly expressed in MCL tissues and some cell lines including Maver, Z138, and Jeko-1, and significantly associated with clinical factors...
Source: Experimental Cell Research - November 13, 2020 Category: Cytology Authors: Zhu MX, Wan WL, Hong Y, Wang YF, Dong F, Jing HM Tags: Exp Cell Res Source Type: research

Leptin modulates gene expression in the heart and cardiomyocytes towards mitigating ischemia-induced damage.
Abstract Leptin, an adipocyte-derived satiety hormone, has been previously linked to cardioprotection. We have shown before that leptin conferred resistance to ischemic damage in the heart in long-lived transgenic αMUPA mice overexpressing leptin compared to the wild type (WT) FVB/N control mice. To better understand the contribution of leptin to the ischemic heart, we measured here the expression of genes encoding leptin and ischemia-related proteins in αMUPA and WT mice in the heart vs adipose tissue after MI. In addition, we investigate gene expression in neonatal rat cardiomyocytes under hypoxia in...
Source: Experimental Cell Research - November 12, 2020 Category: Cytology Authors: Abd Alkhaleq H, Kornowski R, Waldman M, Levy E, Zemel R, Nudelman V, Shainberg A, Miskin R, Hochhauser E Tags: Exp Cell Res Source Type: research

Inactivated STAT5 pathway underlies a novel inhibitory role of EBF1 in chronic lymphocytic leukemia.
This study is to explore the effect of EBF1 on CLL cell progression and its involvement in regulating the signal transducers and activators of transcription 5 (STAT5) pathway. We conducted a correlation analysis between EBF1 and the clinical characteristics of CLL patients. Subsequently, EBF1 was overexpressed by transfection with EBF1 overexpression plasmid and the STAT5 pathway was also blocked by treatment with SH-4-54 in isolated CD20+ B lymphocytes to investigate their roles in the regulation of cellular functions. STAT5, Janus kinase 2 (JAK2) expression and their phosphorylation levels were determined by quantitative...
Source: Experimental Cell Research - November 11, 2020 Category: Cytology Authors: Wang C, Li L, Li M, Shen X, Liu Y, Wang S Tags: Exp Cell Res Source Type: research

Signaling interplay between PARP1 and ROS regulates stress-induced cell death and developmental changes in Dictyostelium discoideum.
In conclusion, we underscore the significant link between PARP1 and ROS in regulating the process of cell death and development in D. discoideum. PMID: 33188850 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 11, 2020 Category: Cytology Authors: Mir H, Rajawat J, Vohra I, Vaishnav J, Kadam A, Begum R Tags: Exp Cell Res Source Type: research

Soluble epoxide hydrolase inhibitors improve angiogenic function of endothelial progenitor cells via ERK/p38-mediated miR-126 upregulation in myocardial infarction mice after exercise.
This study showed that sEHi promoted miR-126 expression, which might be related to the beneficial effect of sEHi on EPC functions in MI mice under exercise conditions, by increasing ERK and p38 MAPK phosphorylation and inhibiting Spred1. PMID: 33188851 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 11, 2020 Category: Cytology Authors: Gui Y, Chen J, Hu J, Liao C, Ouyang M, Deng L, Yang J, Xu D Tags: Exp Cell Res Source Type: research

Retraction notice to "IL-1 β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway" [Exp. Cell Res. (2014) 69-86].
Retraction notice to "IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway" [Exp. Cell Res. (2014) 69-86]. Exp Cell Res. 2020 Nov 11;:112345 Authors: Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M PMID: 33189307 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 11, 2020 Category: Cytology Authors: Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Plasmodium falciparum maturation across the intra-erythrocytic cycle shifts the soft glassy viscoelastic properties of red blood cells from a liquid-like towards a solid-like behavior.
tes B Abstract The mechanical properties of erythrocytes have been investigated by different techniques. However, there are few reports on how the viscoelasticity of these cells varies during malaria disease. Here, we quantitatively map the viscoelastic properties of Plasmodium falciparum-parasitized human erythrocytes. We apply new methodologies based on optical tweezers to measure the viscoelastic properties and defocusing microscopy to measure the erythrocyte height profile, the overall cell volume, and its form factor, a crucial parameter to convert the complex elastic constant into complex shear modulus. The ...
Source: Experimental Cell Research - November 10, 2020 Category: Cytology Authors: Gómez F, Silva LS, Teixeira DE, Agero U, Pinheiro AAS, Viana NB, Pontes B Tags: Exp Cell Res Source Type: research

Telomeres and telomerase in risk assessment of cardiovascular diseases.
Abstract Telomeres are repetitive nucleoprotein structures located at the ends of chromosomes. Reduction in the number of repetitions causes cell senescence. Cells with high proliferative potential age with each replication cycle. Post-mitotic cells (e.g. cardiovascular cells) have a different aging mechanism. During the aging of cardiovascular system cells, permanent DNA damage occurs in the telomeric regions caused by mitochondrial dysfunction, which is a phenomenon independent of cell proliferation and telomere length. Mitochondrial dysfunction is accompanied by increased production of reactive oxygen species a...
Source: Experimental Cell Research - November 7, 2020 Category: Cytology Authors: Boniewska-Bernacka E, Pańczyszyn A, Klinger M Tags: Exp Cell Res Source Type: research

Retraction notice to "MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells"[Exp. Cell Res. 331 (2015) 105-114].
Retraction notice to "MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells"[Exp. Cell Res. 331 (2015) 105-114]. Exp Cell Res. 2020 Nov 06;:112351 Authors: Ozeki N, Hase N, Kawai R, Yamaguchi H, Hiyama T, Kondo A, Nakata K, Mogi M PMID: 33168171 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 6, 2020 Category: Cytology Authors: Ozeki N, Hase N, Kawai R, Yamaguchi H, Hiyama T, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Retraction notice to "Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells" [Exp. Cell Res. 347 (2016) 24-41].
Retraction notice to "Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells" [Exp. Cell Res. 347 (2016) 24-41]. Exp Cell Res. 2020 Nov 06;:112340 Authors: Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, Matsumoto T, Nakata K PMID: 33168172 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 6, 2020 Category: Cytology Authors: Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, Matsumoto T, Nakata K Tags: Exp Cell Res Source Type: research

Retraction notice to "MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells"[Exp. Cell Res. 352 (2017) 63-74].
Retraction notice to "MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells"[Exp. Cell Res. 352 (2017) 63-74]. Exp Cell Res. 2020 Nov 06;:112350 Authors: Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai-Asano R, Nakata K, Mogi M PMID: 33168173 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 6, 2020 Category: Cytology Authors: Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai-Asano R, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

In vitro derived female hPGCLCs are unable to complete meiosis in embryoid bodies.
Abstract The fundamental question about the functionality of in vitro derived human primordial germ cell-like cells remains unanswered, despite ongoing research in this area. Attempts have been made to imitate the differentiation of human primordial germ cells (hPGCs) and meiocytes in vitro from human pluripotent stem cells (hPSCs). A defined system for developing human haploid cells in vitro is the challenge that scientists face to advance the knowledge of human germ cell development. To develop human primordial germ cell-like cells (hPGCLCs) from human pluripotent stem cells (hPSCs) that are capable of giving ri...
Source: Experimental Cell Research - November 5, 2020 Category: Cytology Authors: Abdyyev VK, Sant DW, Kiseleva EV, Spangenberg VE, Kolomiets OL, Andrade NS, Dashinimaev EB, Vorotelyak EA, Vasiliev AV Tags: Exp Cell Res Source Type: research

Blood coagulation factor VIII D1241E polymorphism leads to a weak malectin interaction and reduction of factor VIII posttranslational modification and secretion.
This study investigated the mechanism by which FVIII is translated and transported into the endoplasmic reticulum (ER) and processed in the Golgi apparatus before secretion using an in vitro cell model. HEK-293T cells were transfected with vectors carrying wild-type (WT) FVIII or polymorphic FVIII D1241E for coexpression with ER lectins and treatment with tunicamycin (an N-linked glycosylation inhibitor), 1-deoxynojirimycin (an alpha-glucosidase inhibitor), endoglycosidase H, or MG132 (Cbz-Leu-Leu-leucinal; a proteasome inhibitor). The data showed that the minor allele of FVIII D1241E was able to reduce FVIII secretion int...
Source: Experimental Cell Research - October 31, 2020 Category: Cytology Authors: Jiang N, Xiao Y, Liu Y, Liu W, Liu S Tags: Exp Cell Res Source Type: research

Retraction notice to "IL-1 β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells" [Exp. Cell Res. 323 (2014) 165-177].
Retraction notice to "IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells" [Exp. Cell Res. 323 (2014) 165-177]. Exp Cell Res. 2020 Oct 30;:112339 Authors: Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M, Nakamura H PMID: 33138979 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 30, 2020 Category: Cytology Authors: Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M, Nakamura H Tags: Exp Cell Res Source Type: research

Retraction notice to "Autophagy-related gene 5 and Wnt5 signaling pathway requires differentiation of embryonic stem cells into odontoblast-like cells" [Exp. Cell Res. 341 (2016) 92-104].
Retraction notice to "Autophagy-related gene 5 and Wnt5 signaling pathway requires differentiation of embryonic stem cells into odontoblast-like cells" [Exp. Cell Res. 341 (2016) 92-104]. Exp Cell Res. 2020 Oct 30;:112338 Authors: Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K PMID: 33138980 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 30, 2020 Category: Cytology Authors: Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K Tags: Exp Cell Res Source Type: research

The clock regulator Bmal1 protects against muscular dystrophy.
Abstract The muscle-intrinsic clock machinery is required for the maintenance of muscle growth, remodeling and function. Our previous studies demonstrated that the essential transcription activator of the molecular clock feed-back loop, Brain and Muscle Arnt-Like 1(Bmal1), plays a critical role in myogenic progenitor behavior to promote and regenerative myogenesis. Using genetic approaches targeting Bmal1 in the DMDmdx (mdx) dystrophic mouse model, here we report that the loss of Bmal1 function significantly accelerated dystrophic disease progression. In contrast to the mild dystrophic changes in mdx mice, the gen...
Source: Experimental Cell Research - October 29, 2020 Category: Cytology Authors: Gao H, Xiong X, Lin Y, Chatterjee S, Ma K Tags: Exp Cell Res Source Type: research

Augmenter of Liver Regeneration (ALR) regulates bile acid synthesis and attenuates bile acid-induced apoptosis via glycogen synthase kinase-3 β (GSK-3β) inhibition.
Augmenter of Liver Regeneration (ALR) regulates bile acid synthesis and attenuates bile acid-induced apoptosis via glycogen synthase kinase-3β (GSK-3β) inhibition. Exp Cell Res. 2020 Oct 24;397(1):112343 Authors: Ibrahim S, Dayoub R, Saberi V, Buchner M, Melter M, Weiss TS Abstract Bile acid synthesis is restricted to hepatocytes and is rate-limited by CYP7A1 (cholesterol 7α hydroxylase). CYP7A1 expression undergoes tight regulation and is repressed after partial hepatectomy to prevent the accumulation of toxic bile acids. Augmenter of Liver Regeneration (ALR) is a hepatotrophic factor...
Source: Experimental Cell Research - October 24, 2020 Category: Cytology Authors: Ibrahim S, Dayoub R, Saberi V, Buchner M, Melter M, Weiss TS Tags: Exp Cell Res Source Type: research

Skp2 regulates DNA damage repair and apoptosis via interaction with Ku70.
CONCLUSION: The direct interaction between Skp2 and Ku70 proteins mediates the DNA damage repair and cellular apoptosis by regulating Ku70 stability and function via post-translational modification. The molecular mechanisms how Skp2 stabilize Ku70 would be clarified in our following research work. PMID: 33132134 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 22, 2020 Category: Cytology Authors: Jia J, Yan D, Wang Y, Liu M, Jia Z, Luo L, Ren J, Fang J, Yuan H, Zhao Y, Song Y, He Y, Wang X Tags: Exp Cell Res Source Type: research

siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.
In this study, we focused on alanine aminopeptidase (APP) and investigated the function of APP in C2C12 myoblast proliferation and differentiation. In myoblasts and myotubes, APP was mainly localized in the cell membrane as well as expressed at low levels in the cytoplasm and nucleus. The reduction of the APP enzymatic activity impaired the cell cycle progression in C2C12 myoblasts. In addition, apoptosis was induced after APP-knockdown. Finally, myogenic differentiation was also delayed in the APP-suppressed myoblasts. These findings indicate that APP is required for myoblast proliferation and differentiation. PMID: ...
Source: Experimental Cell Research - October 19, 2020 Category: Cytology Authors: Osana S, Kitajima Y, Suzuki N, Xu Y, Murayama K, Nagatomi R Tags: Exp Cell Res Source Type: research

TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.
In conclusion, TRIM16 alleviated oxidative damage in hPDLSCs via the activation of PICOT/Akt/Nrf2 pathway, suggesting that TRIM16 could be a promising target to develop effective therapies for periodontitis. PMID: 33091421 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 19, 2020 Category: Cytology Authors: Zhao Y, Liu H, Xi X, Chen S, Liu D Tags: Exp Cell Res Source Type: research

Role of RIN1 on telomerase activity driven by EGF-Ras mediated signaling in breast cancer.
In this study, we tested the hypothesis that RIN1, a Ras effector protein and Rab5 guanine nucleotide exchange factor, controls several signaling molecules leading to the modulation of the telomerase activity; thus, allowing proper cell proliferation. We report that expression of RIN1 completely blocked proliferation of MCF-12 A and MCF-7 cells, while partially inhibited proliferation of MDA-MB-231 cells upon EGF stimulation. Furthermore, expression of the C-terminal region of RIN1 selectively plays a critical role in the inhibition of the proliferation of MDA-MB-231 cells. However, this inhibitory effect was speci...
Source: Experimental Cell Research - October 15, 2020 Category: Cytology Authors: Zhang W, Veisaga ML, Barbieri MA Tags: Exp Cell Res Source Type: research

Clearance of neurotoxic peptides and proteins by meningothelial cells.
This study reveals for the first time the function of MECs as scavengers of neurotoxic Aβ and α-synuclein, thereby connecting these cells to neuroprotective processes and suggesting a new mechanism and pathway for clearing neurotoxic substances from the CSF. PMID: 33068559 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 14, 2020 Category: Cytology Authors: Hemion C, Li J, Kohler C, Scholl HPN, Meyer P, Killer HE, Neutzner A Tags: Exp Cell Res Source Type: research

LncRNA DANCR upregulation induced by TUFT1 promotes malignant progression in triple negative breast cancer via miR-874-3p-SOX2 axis.
Abstract Triple negative breast cancer (TNBC) is a subtype of breast cancer with poorest survival outcome and is prone to metastasis. TUFT1 and the long non-coding RNA (lncRNA), DANCR, play vital roles in metastasis and progression of various cancers. However, the correlation between TUFT1 and DANCR in TNBC and their downstream molecular mechanisms are still undetermined. We demonstrated that upregulation of TUFT1 in TNBC was related to a worse survival in TNBC patients. The TNBC cells invasiveness was augmented by TUFT1 in a dose-dependent manner, while inhibiting TUFT1 repressed the invasiveness. Particularly, t...
Source: Experimental Cell Research - October 13, 2020 Category: Cytology Authors: Wu G, Zhou H, Li D, Zhi Y, Liu Y, Li J, Wang F Tags: Exp Cell Res Source Type: research

RNA helicase DDX5 suppresses IFN-I antiviral innate immune response by interacting with PP2A-C β to deactivate IRF3.
RNA helicase DDX5 suppresses IFN-I antiviral innate immune response by interacting with PP2A-Cβ to deactivate IRF3. Exp Cell Res. 2020 Oct 13;:112332 Authors: Zan J, Xu R, Tang X, Lu M, Xie S, Cai J, Huang Z, Zhang J Abstract DEAD-box (DDX) helicases are critical for recognizing viral nucleic acids to regulate antiviral innate immunity. Although DDX5 has been reported to participate in various virus infection, whether DDX5 regulates innate immune responses and its underlying mechanisms are still unknown. Here, we report that DDX5 is a negative regulator of type I IFN (IFN-I) production in antivir...
Source: Experimental Cell Research - October 13, 2020 Category: Cytology Authors: Zan J, Xu R, Tang X, Lu M, Xie S, Cai J, Huang Z, Zhang J Tags: Exp Cell Res Source Type: research

The alpha-1,2 fucosylated tubule system of DU145 prostate cancer cells is derived from a partially fragmented Golgi complex and its formation is actin-dependent.
Abstract In previous work, we showed that highly proliferative cells and cancer cells, but not cells with normal growth rate, have tubules rich in alpha-1,2 fucosylated epitopes that extend radially from the nucleus to the cell periphery and form an unusual uptake system. The importance of alpha-1,2 fucosylation in forming tubules was demonstrated by proving that down-regulating the two corresponding fucosyltransferases (FUT1 and FUT2) causes tubule fragmentation. Here, we present evidence that in the prostate cancer cell line DU145, the tubules arise in actively growing cells from vesicles in the medial and trans...
Source: Experimental Cell Research - October 13, 2020 Category: Cytology Authors: Domenico N, Antonietta C, Rosati F, Della Giovampaola C Tags: Exp Cell Res Source Type: research

Down-regulation of Aquaporin-1 mediates a microglial phenotype switch affecting glioma growth.
Abstract Aquaporin 1 (AQP1), a transmembrane protein that forms water channels, has previously been shown to facilitate growth and progression of many types of tumors by modulating tumor cell migration, proliferation and angiogenesis. Here, we determined the impact of AQP1 expression in the tumor environment on the progression of brain tumors. Primary microglia from wild type(WT) and AQP1 knockout(KO) mice were used to test AQP1 effect on microglia function by using Western blot, quantative PCR, in an experimental in vivo mouse glioma model and organotypic brain slice culture. Deletion of AQP1 in the host tissue s...
Source: Experimental Cell Research - October 12, 2020 Category: Cytology Authors: Hu F, Huang Y, Semtner M, Zhao K, Tan Z, Dzaye O, Kettenmann H, Shu K, Lei T Tags: Exp Cell Res Source Type: research

NET-associated citrullinated histones promote LDL aggregation and foam cell formation in vitro.
Abstract Neutrophils have been recently identified in the atherosclerotic lesion and they can release neutrophil extracellular trap (NET) under the pro-inflammatory conditions prevailing in the lesion. Citrullinated histones (Cit-histones) are the major type of citrullinated proteins associated with NET release. Since elevated levels of citrullinated proteins have been detected in inflammatory diseases including atherosclerosis, this study analysed the role played by NET and Cit-histones in different atherogenic events in vitro. First, neutrophil recruitment and NET release in the presence of low-density lipoprote...
Source: Experimental Cell Research - October 12, 2020 Category: Cytology Authors: Haritha VH, George A, Shaji BV, Anie Y Tags: Exp Cell Res Source Type: research

Exosomes from adipose-derived stem cells attenuate UVB-induced apoptosis, ROS, and the Ca2+ level in HLEC cells.
Abstract Cartilage acid protein 1 (CRTAC1) encodes a protein containing the Ca2+binding domain, which can promote apoptosis of human lens epithelial cells (HLECs) induced by ultraviolet B radiation. Exosomes secreted from adipose-derived stem cells (ASC-exo) have been used to treat many diseases, but the effect of ASC-exo on cataracts has not been established. We hypothesized that ASC-exo has a therapeutic effect on cataracts by regulating CRTAC1. We established the UVB-induced injured HLECs model to test the interactions between CRTAC1 and miR-10a-5p, and the effect on the Ca2+ level and reactive oxygen species (...
Source: Experimental Cell Research - October 9, 2020 Category: Cytology Authors: Hong Y, Sun Y, Rong X, Li D, Lu Y, Ji Y Tags: Exp Cell Res Source Type: research

Noisy stimulation effect in calcium dynamics on cardiac cells.
This study investigates whether noise can induce changes in calcium dynamics on the two main heart cell types: pacemaker and myocardial cells, when stimuli with periodic electrical signals are disturbed by Gaussian white noise. Calcium dynamic parameters were obtained using imaging signals. Our results show that low intensities of noise favor amplitude and raise rate calcium dynamics, although our results show that the pacemaker cells are not affected by a noisy stimulus. Altogether, these findings suggest that noise plays a key role in calcium dynamics. PMID: 33039368 [PubMed - as supplied by publisher] (Source: Expe...
Source: Experimental Cell Research - October 8, 2020 Category: Cytology Authors: Ramírez Hurtado AL, Martínez FV, Diaz Galindo CA, Cuellar KG, Villareal Reyna SZ, Sánchez Herrera DP, Rodríguez González J Tags: Exp Cell Res Source Type: research

Ubiquitin-specific protease 4 predicts an unfavorable prognosis and promotes malignant behaviors in vitro in pancreatic cancer.
Abstract Ubiquitin-specific protease 4 (USP4), has been reported to participate in the progression of various cancers due to its role in post-translational modulation. However, the prognostic significance and mechanism of USP4 in pancreatic cancer (PC) have not been well elucidated before. In the present study, we found that USP4 expression was higher in PC tissues than that in adjacent normal tissues and PC patients with high level of USP4 expression have a poor prognosis via immunohistochemistry and bioinformatics analyses. In vitro study showed that knockdown of USP4 inhibited PC cells proliferation, migration ...
Source: Experimental Cell Research - October 7, 2020 Category: Cytology Authors: Wang Y, Zhou L, Lu J, Jiang B, Liu C, Liang Z, Zhou W, Guo J Tags: Exp Cell Res Source Type: research

Elucidating the framework for specification and determination of the embryonic retina.
This study provides essential groundwork for defining the mechanisms for how these important developmental transitions occur. PMID: 33031807 [PubMed - as supplied by publisher] (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 5, 2020 Category: Cytology Authors: Louie SH, Fisher M, Grainger RM Tags: Exp Cell Res Source Type: research

Tumor-associated macrophages induce PD-L1 expression in gastric cancer cells through IL-6 and TNF- ɑ signaling.
In this study, we found a high correlation between macrophage infiltration and PD-L1 expression in gastric cancer (GC) specimens. Further study revealed that infiltrated macrophages released the proinflammatory cytokines TNF-ɑ and IL-6, which induced PD-L1 expression in tumor cells. The release of TNF-ɑ and IL-6 activated the NF-kB and STAT3 signaling pathway to regulate PD-L1 expression. TNF-α, p-65 and STAT3 expression in cancer patients has prognostic value in stomach adenocarcinoma. Furthermore, infiltrated macrophages can also promote GC cell proliferation by inducing PD-L1 expression in GC cells. Taken togeth...
Source: Experimental Cell Research - October 5, 2020 Category: Cytology Authors: Ju X, Zhang H, Zhou Z, Chen M, Wang Q Tags: Exp Cell Res Source Type: research

Molecular mechanisms of KDM5A in cellular functions: Facets during development and disease.
Abstract Gene expression is influenced at many layers by a fine-tuned crosstalk between multiple extrinsic signalling pathways and intrinsic regulatory molecules that respond to environmental stimuli. Epigenetic modifiers like DNA methyltransferases, histone modifying enzymes and chromatin remodellers are reported to act as triggering factors in many scenarios by exhibiting their control over most of the cellular processes. These epigenetic players can either directly regulate gene expression or interact with some effector molecules that harmonize the expression of downstream genes. One such epigenetic regulator w...
Source: Experimental Cell Research - September 29, 2020 Category: Cytology Authors: Kirtana R, Manna S, Patra SK Tags: Exp Cell Res Source Type: research