A β42 treatment of the brain side reduced the level of flotillin from endothelial cells on the blood side via FGF-2 signaling in a blood-brain barrier model
In this study, to elucidate whether Aβ accumulation in the brain has an effect on the blood flotillin level, we used our previously established blood-brain barrier (BBB) culture model using microvascular endothelial cells obtained from human induced pluripotent stem cells (iBMECs) and astrocytes prepared from rat cortex. In this BBB model with iBMECs plated on the upper compartment (blood side) and astrocytes plated on the lower compartment (brain side), the trans-endothelial electrical resistance values are high (over 1500 Ωm2) and stable during experiments. We found that the addition of Aβ42 (0.5 and 2 µM) to the bra...
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Tomohisa Nakamura Tadahiro Hashita Yuxin Chen Yuan Gao Yang Sun Sadequl Islam Hiroyuki Sato Yasuyuki Shibuya Kun Zou Tamihide Matsunaga Makoto Michikawa Source Type: research

Probing into the Flap-Dimer Dynamics of the Mycobacterium Tuberculosis Kasa Enzyme Binding Landscape provides The Underlying Inhibitory Mechanisms Of JSF-3285 And 5G
CONCLUSION: These findings provide a background for a new structure-based design of novel KasA inhibitors.PMID:36698229 | DOI:10.2174/1568026623666230125124433 (Source: Molecular Medicine)
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Adeniyi T Adewumi Wande M Oluyemi Nonhlanhla Adewumi Yemi A Adekunle Mohamed Issa Alahmdi Nader E Abo-Dyai Mahmoud E S Soliman Source Type: research

A nitrous oxide abuser presenting with cerebral venous thrombosis: A case report
Med Int (Lond). 2022 Jun 7;2(3):20. doi: 10.3892/mi.2022.45. eCollection 2022 May-Jun.ABSTRACTThe present study describes the case of a 25-year-old male patient who presented to the emergency department with severe headache and vertigo lasting for 3 days. The patient did not have a recent history of trauma. He was vaccinated with a second dose of the AstraZeneca COVID-19 vaccine ~1 month prior, and he suffered from a vitamin B12 deficiency due to nitrous oxide abuse. Upon an examination of his vital signs, he was found to have a body temperature of 36.4˚C, a pulse rate of 64 beats per minute, a respiratory rate of 18 brea...
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Shum-Shin Lin I-Wei Fan Chun-Yu Chen Yu-Jang Su Source Type: research

The molecular biology of peritoneal metastatic disease
EMBO Mol Med. 2023 Jan 26:e15914. doi: 10.15252/emmm.202215914. Online ahead of print.ABSTRACTPeritoneal metastases are a common form of tumor cell dissemination in gastrointestinal malignancies. Peritoneal metastatic disease (PMD) is associated with severe morbidity and resistance to currently employed therapies. Given the distinct route of dissemination compared with distant organ metastases, and the unique microenvironment of the peritoneal cavity, specific tumor cell characteristics are needed for the development of PMD. In this review, we provide an overview of the known histopathological, genomic, and transcriptomic ...
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Sanne Bootsma Maarten F Bijlsma Louis Vermeulen Source Type: research

Rare hepatosplenic gamma-delta T-cell lymphoma detected by < sup > 18 < /sup > F-FDG PET/CT
Rev Esp Med Nucl Imagen Mol (Engl Ed). 2023 Jan 23:S2253-8089(23)00003-4. doi: 10.1016/j.remnie.2023.01.003. Online ahead of print.NO ABSTRACTPMID:36702381 | DOI:10.1016/j.remnie.2023.01.003 (Source: Molecular Medicine)
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Xia Lu Jigang Yang Source Type: research

Extended therapy with [ < sup > 177 < /sup > Lu]Lu-PSMA-617 in responding patients with high-volume metastatic castration-resistant prostate cancer
CONCLUSION: Extended radioligand therapy is a feasible treatment option in patients with high-volume residual tumor after the completion of standard treatment with six cycles of [177Lu]Lu-PSMA-617. Improved survival and the acceptable safety profile warrant further investigation of the concept of additional cycles in selected patients.PMID:36702927 | DOI:10.1007/s00259-023-06119-1 (Source: Molecular Medicine)
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Nicolai Mader Christina Nguyen Ngoc Bilge Kirkg öze Justus Baumgarten Daniel Groener Konrad Klimek Christian Happel Nikolaos Tselis Felix K H Chun Frank Gr ünwald Amir Sabet Source Type: research

[ < sup > 18 < /sup > F]FDG PET in conditions associated with hyperkinetic movement disorders and ataxia: a systematic review
CONCLUSION: In all conditions with hyperkinetic movement disorders, hypo- or hypermetabolism was found in multiple, partly overlapping brain regions, and clinical characteristics often correlated with glucose metabolism. For some movement disorders, [18F]FDG PET metabolic changes reflected the effect of treatment.PMID:36702928 | DOI:10.1007/s00259-023-06110-w (Source: Molecular Medicine)
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Elze R Timmers Marrit R Klamer Ramesh S Marapin Adriaan A Lammertsma Bauke M de Jong Rudi A J O Dierckx Marina A J Tijssen Source Type: research

Urine-based multi-omic comparative analysis of COVID-19 and bacterial sepsis-induced ARDS
CONCLUSION: In summary, our results provide evidence for significant molecular differences in ARDS patients from different etiologies and a potential synergy of extracellular matrix molecules, inflammation, and mitochondrial dysfunction in ARDS pathogenesis. The proteomic mortality signature should be further investigated in future studies to develop prediction models for COVID-19 patient outcomes.PMID:36703108 | DOI:10.1186/s10020-023-00609-6 (Source: Molecular Medicine)
Source: Molecular Medicine - January 26, 2023 Category: Molecular Biology Authors: Richa Batra Rie Uni Oleh M Akchurin Sergio Alvarez-Mulett Luis G G ómez-Escobar Edwin Patino Katherine L Hoffman Will Simmons William Whalen Kelsey Chetnik Mustafa Buyukozkan Elisa Benedetti Karsten Suhre Edward Schenck Soo Jung Cho Augustine M K Choi Fr Source Type: research

Lack of the human choline transporter-like protein SLC44A2 causes hearing impairment and a rare red blood phenotype
EMBO Mol Med. 2023 Jan 25:e16320. doi: 10.15252/emmm.202216320. Online ahead of print.ABSTRACTBlood phenotypes are defined by the presence or absence of specific blood group antigens at the red blood cell (RBC) surface, due to genetic polymorphisms among individuals. The recent development of genomic and proteomic approaches enabled the characterization of several enigmatic antigens. The choline transporter-like protein CTL2 encoded by the SLC44A2 gene plays an important role in platelet aggregation and neutrophil activation. By investigating alloantibodies to a high-prevalence antigen of unknown specificity, found in pati...
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: B érengère Koehl C édric Vrignaud Mahmoud Mikdar Thankam S Nair Lucy Yang Seyve Landry Guy Laiguillon Claudine Giroux-Lathuile Sophie Anselme-Martin Hanane El Kenz Olivier Hermine Narla Mohandas Jean Pierre Cartron Yves Colin Olivier Detante Rapha ël Source Type: research

Two sisters with cardiac-urogenital syndrome secondary to pathogenic splicing variant in the MYRF gene with unaffected parents: A case of gonadal mosaicism?
CONCLUSIONS: To our knowledge, this is the first published case of familial cardiac-urogenital syndrome indicating gonadal mosaicism.PMID:36695166 | DOI:10.1002/mgg3.2139 (Source: Molecular Medicine)
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: Katerina Slaba Marta Jezova Petra Pokorna Hana Palova Jana Tuckova Jan Papez Dagmar Prochazkova Petr Jabandziev Ondrej Slaby Source Type: research

[Corrigendum] NEP1 ‑40 promotes myelin regeneration via upregulation of GAP‑43 and MAP‑2 expression after focal cerebral ischemia in rats
Mol Med Rep. 2023 Mar;27(3):58. doi: 10.3892/mmr.2023.12945. Epub 2023 Jan 25.ABSTRACTSubsequently to the publication of this paper, an interested reader drew to the authors' attention that, in Fig. 4A on p. 6 showing the effects of NEP1‑40 on MBP expression as determined via immunohistochemical analysis, certain of the data panels appeared to be overlapping, such that they may have been derived from the same original source. After having examined their original data, the authors have realized that these data panels were inadvertently assembled incorrectly. A corrected version of Fig. 4 is shown below, incorporating data...
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: Hong Zhao Zhen-Dong Liu Yong-Bo Zhang Xiao-Yu Gao Cui Wang Yi Liu Xun-Fen Wang Source Type: research

Resveratrol-Loaded Dipalmitoylphosphatidylcholine Liposomal Large Porous Microparticle Inhalations for the Treatment of Bacterial Pneumonia Caused by < em > Acinetobacter baumannii < /em >
Conclusion: The RDLPMs showed good powder properties for pulmonary delivery. The RDLPMs may inhibit the nuclear factor kappa-B pathway and downregulate the expression of cytokines downstream of tumor necrosis factor-α and interleukin-1β. As well as, RDLPMs demonstrated some antibacterial properties against A. baumannii bacteria. Re, when delivered in RDLPMs as a dry powder inhaler, is a promising substitute for antibiotics in the treatment of A. baumannii pneumonia.PMID:36695669 | DOI:10.1089/jamp.2021.0049 (Source: Molecular Medicine)
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: Zicheng Yu Tingting Wu Xiaoyan Liu Hongjun Chen Chunxia Ren Lifei Zhu Source Type: research

Prognostic value of preoperative [ < sup > 68 < /sup >  Ga]Ga-FAPI-04 PET/CT in patients with resectable pancreatic ductal adenocarcinoma in correlation with immunohistological characteristics
CONCLUSION: The in vivo [68 Ga]Ga-FAPI-04 uptake in localized PDAC showed a significant correlation with ex vivo FAP expression and aggressive pathological characteristics. [68 Ga]Ga-FAPI-04 PET/CT also presented a potential for postoperative prognostication of PDAC. Elevated fibroblast activity induced by obstructive pancreatitis might be associated with the patient's survival.PMID:36695823 | DOI:10.1007/s00259-022-06100-4 (Source: Molecular Medicine)
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: Jie Ding Jiangdong Qiu Zhixin Hao Hua Huang Qiaofei Liu Wenjing Liu Chao Ren Marcus Hacker Taiping Zhang Wenming Wu Li Huo Xiang Li Source Type: research

< sup > 11 < /sup > C-methionine PET imaging characteristics in children with diffuse intrinsic pontine gliomas and relationship to survival and H3 K27M mutation status
CONCLUSION: Although several imaging features were significantly associated with PFS and OS, only MTV, indicating the size of the active tumour, was identified as a strong independent prognostic factor.PMID:36697961 | DOI:10.1007/s00259-022-06105-z (Source: Molecular Medicine)
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: Xiaobin Zhao Deling Li Zhen Qiao Kai Wang Qian Chen Changcun Pan Yuliang Wu Dan Xiao Tianshu Xi Liwei Zhang Lin Ai Source Type: research

Lack of the human choline transporter-like protein SLC44A2 causes hearing impairment and a rare red blood phenotype
EMBO Mol Med. 2023 Jan 25:e16320. doi: 10.15252/emmm.202216320. Online ahead of print.ABSTRACTBlood phenotypes are defined by the presence or absence of specific blood group antigens at the red blood cell (RBC) surface, due to genetic polymorphisms among individuals. The recent development of genomic and proteomic approaches enabled the characterization of several enigmatic antigens. The choline transporter-like protein CTL2 encoded by the SLC44A2 gene plays an important role in platelet aggregation and neutrophil activation. By investigating alloantibodies to a high-prevalence antigen of unknown specificity, found in pati...
Source: Molecular Medicine - January 25, 2023 Category: Molecular Biology Authors: B érengère Koehl C édric Vrignaud Mahmoud Mikdar Thankam S Nair Lucy Yang Seyve Landry Guy Laiguillon Claudine Giroux-Lathuile Sophie Anselme-Martin Hanane El Kenz Olivier Hermine Narla Mohandas Jean Pierre Cartron Yves Colin Olivier Detante Rapha ël Source Type: research