Bio-Membrane SELEX as a new approach for selecting ss-DNA aptamers that bind to the hydatid cyst laminated layer
CONCLUSION: In this investigation, we propose a novel method to determine specific aptamers. Bio-Membrane SELEX, could be assumed as a practical and sensitive method for aptamer selection. Selected aptamers in this study possibly may be used for specific HC antigens detection.PMID:35578872 | DOI:10.2174/1566524022666220516140451 (Source: Molecular Medicine)
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Azar Balouti Dehkordi Hossein Khanahmad Yadollah Azizi Mina Mirian Nader Pestehchian Hossein Yousofi Darani Source Type: research

Anti-TLR4 IgG2 prevents acetaminophen-induced acute liver injury through the Toll-like receptor 4/MAPKs signaling pathway in mice
CONCLUSION: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of anti-TLR4 IgG2 in APAP-induced hepatotoxicity may be due to its anti-oxidation, anti-apoptosis, and anti-inflammation effects through inhibition of the TLR4/MAPKs signaling axis.PMID:35578873 | DOI:10.2174/1566524022666220516141728 (Source: Molecular Medicine)
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Chuanxia Yao Maorong Wang Jin Zhu Yiwen Wang DanDan Gong Tian Feng Yaqiong Zhang Chunhui Wang Source Type: research

Cofilin-1 participates in the hyperfunction of myeloid dendritic cells in patients with severe aplastic anaemia
J Cell Mol Med. 2022 May 17. doi: 10.1111/jcmm.17359. Online ahead of print.ABSTRACTCofilin-1 interacts with actin to regulate cell movement. The importance of cofilin-1 in immunity has been established, and its involvement in a number of autoimmune diseases has been confirmed. However, its role in severe aplastic anaemia (SAA) remains elusive. Thus, the aim of the current study was to investigate the role of cofilin-1 in patients with SAA. Flow cytometry, Western blotting and real-time quantitative reverse transcription-polymerase chain reaction were performed to detect the mRNA and protein expression of cofilin-1 in myel...
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Yingying Sun Yu Zhang Hong Yu Huaquan Wang Zonghong Shao Chunyan Liu Source Type: research

Olverembatinib inhibits SARS-CoV-2-Omicron variant-mediated cytokine release in human peripheral blood mononuclear cells
EMBO Mol Med. 2022 May 17:e15919. doi: 10.15252/emmm.202215919. Online ahead of print.NO ABSTRACTPMID:35579119 | DOI:10.15252/emmm.202215919 (Source: Molecular Medicine)
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Marina Chan Eric C Holland Taranjit S Gujral Source Type: research

The emerging role of LncRNA FENDRR in multiple cancer: A review study
Curr Mol Med. 2022 May 9. doi: 10.2174/1566524022666220509122505. Online ahead of print.ABSTRACTLong noncoding RNAs (lncRNAs) are prominent as crucial regulators of tumor establishment and are repeatedly dysregulated in multiple cancers. Therefore, lncRNAs have been identified to play an essential function in carcinogenesis and progression of cancer at genetic and epigenetic levels. FENDRR (fetal-lethal noncoding developmental regulatory RNA) as an LncRNA is a hallmark of various malignancies. FENDRR is crucial for multiple organs' development such as lung and heart. The effects of FENDRR under signaling pathways in differ...
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Hoda Fazaeli Azar Sheikholeslami Fatemeh Ghasemian Elaheh Amini Mohsen Sheykhhasan Source Type: research

Ferroptosis and cancer immunotherapy
Curr Mol Med. 2022 May 9. doi: 10.2174/1566524022666220509124608. Online ahead of print.ABSTRACTTraditional treatment strategies of cancer are far from satisfaction. As a non-apoptotic cell death process and owning to the characteristics of iron-dependent lipid peroxide accumulation, ferroptosis has become a new target of tumor treatment. Numerous studies have proved that ferroptosis could enhance the immunogenicity of cancer and interact with immune cells. Cancer antigens, exposed to cancer cells underwent ferroptosis, effectively improve the immunogenicity of the tumor microenvironment and promote the activation and matu...
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Jumei Yin Xingqi Meng Lixuan Peng Wei Xie Xuan Liu Weiguo He Suyun Li Source Type: research

Combination of FOXD1 and Plk2: A novel biomarker for predicting unfavourable prognosis of colorectal cancer
J Cell Mol Med. 2022 May 17. doi: 10.1111/jcmm.17361. Online ahead of print.ABSTRACTColorectal cancer (CRC) is a worldwide disease with worse survival. Our objective is to identify previously unrecognized prognostic factors to better evaluate disease progression. Seven GEO datasets were collected and analysed using R software, followed by KEGG enrichment analysis and TFs network construction. LASSO-COX analysis was performed to select the most useful prognostic features. COX model was used to analyse prognostic factors associated with OS. The survival curve was constructed using Kaplan-Meier analysis. A Nomogram model was ...
Source: Molecular Medicine - May 17, 2022 Category: Molecular Biology Authors: Yaping Zong Yiming Miao Wenchang Li Minhua Zheng Zhuoqing Xu Han Gao Wenqing Feng Zifeng Xu Jingkun Zhao Lifei Shen Aiguo Lu Source Type: research

Functional validation of a novel AAAS variant in an atypical presentation of Allgrove syndrome
CONCLUSIONS: Review of the patient's clinical, pathological, and genetic findings resulted in a diagnosis of Triple A syndrome.PMID:35570467 | DOI:10.1002/mgg3.1966 (Source: Molecular Medicine)
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Erica L Macke Joel A Morales-Rosado Sarah K Macklin-Mantia Christopher T Schmitz Bj örn Oskarsson Eric W Klee Klaas J Wierenga Source Type: research

Natural flavonoid quercetin enhances the anti-inflammatory effects of aspirin in a preeclampsia-like rat model induced by lipopolysaccharide
CONCLUSION: This study suggested that quercetin may serve as an excellent supplement to aspirin in preventing or treating patients with preeclampsia.PMID:35570539 | DOI:10.2174/1566524022666220513111637 (Source: Molecular Medicine)
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Dan Chen Shuangyan Yang Jie Ding Ailing Liu Source Type: research

Verapamil regulates the macrophage immunity to Mycobacterium tuberculosis through NF- κB signaling
CONCLUSIONS: Verapamil enhanced macrophage immunity via the NF-κB pathway, and its effects on cytokines expression may be achieved by Verapamil's regulation on intracellular calcium signaling.PMID:35570540 | DOI:10.2174/1566524022666220513092244 (Source: Molecular Medicine)
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Wenping Gong Ruina Cui Lele Song Yourong Yang Junxian Zhang Yan Liang Xuejuan Bai Jie Wang Lan Wang Xueqiong Wu Weiguo Zhao Source Type: research

Hypoxia-Inducible Factors Based Single Nucleotide Polymorphism in Breast Cancer with More Cancer Susceptibility
Curr Mol Med. 2022 May 13. doi: 10.2174/1566524022666220513124853. Online ahead of print.ABSTRACTHypoxia-inducible factors (HIFs) are a collection of transcriptional factors that engage in the regulation of oxygen homeostasis. They are a hypoxia-responsive stress factor whose expression is linked to tumor growth and angiogenesis. HIF is a crucial player in the progression of breast cancer. Patients with high levels of hypoxia-inducible HIFs in their primary tumor biopsies had a higher chance of metastasis, the leading cause of breast cancer-related death. HIF polymorphisms have been shown in several epidemiological studies...
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Suman Kumar Ray Sukhes Mukherjee Source Type: research

Reduced Melatonin Levels May Facilitate Glioblastoma Initiation in the Subventricular Zone
Expert Rev Mol Med. 2022 May 16:1-8. doi: 10.1017/erm.2022.15. Online ahead of print.NO ABSTRACTPMID:35570582 | DOI:10.1017/erm.2022.15 (Source: Molecular Medicine)
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Majid Ghareghani Kazem Zibara Russel J Rieter Serge Rivest Source Type: research

Alternatively activated macrophages at the recipient site improve fat graft retention by promoting angiogenesis and adipogenesis
This study aimed to trace host-derived macrophages and to evaluate the effects of macrophage infiltration at the recipient site during the early stage on long-term fat graft retention. In our novel mouse model, all mice underwent simulated liposuction and were divided into 2 groups. The fat procurement plus grafting (Pro-Grafting) group was engrafted with prepared fat (0.3 ml). The pro-Grafting+M2 group was engrafted with prepared fat (0.3 ml) mixed with 1.0 × 106 GFP+M0 macrophages, and then, 2 ng IL-4 was injected into the grafts on Day 3. In addition, 1.0 × 106 GFP+M0 macrophages were injected into the tail vein for t...
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Ye Li Xinyao Chen Lin Liu Yunzi Chen Xin Bi Yuting Chen Jialiang Zou Zijue Wang Ziqing Dong Feng Lu Source Type: research

ASC nanobodies to counteract the consequences of inflammasome activation
EMBO Mol Med. 2022 May 16:e16087. doi: 10.15252/emmm.202216087. Online ahead of print.ABSTRACTInflammasomes are multiprotein complexes that signal by oligomerizing the apoptosis speck-like protein with caspase recruitment and activator domain (ASC) and are involved in multiple inflammatory, metabolic and degenerative diseases. Pharmacological targeting of specific inflammasomes with small molecules is leading to the development of novel drugs for most common diseases. The targeting of ASC oligomers will result in a pan-inflammasome treatment. In their study, Bertheloot et al (2022) developed specific anti-ASC nanobodies an...
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Sahil Adriouch Pablo Pelegrin Source Type: research

Convergent Molecular Pathways in Type 2 Diabetes Mellitus and Parkinson's Disease: Insights into Mechanisms and Pathological Consequences
Mol Neurobiol. 2022 May 16. doi: 10.1007/s12035-022-02867-7. Online ahead of print.ABSTRACTType 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by hyperglycemic conditions. A higher risk of developing Parkinson's disease (PD) in patients with T2DM has become evident in recent years. However, the molecular mechanisms underlying the interplay between T2DM and PD pathogenesis remain unknown. Nevertheless, emerging epidemiological studies have demonstrated many common molecular pathways that play an essential role in regulating normal cellular functioning are independently implicated in the progression...
Source: Molecular Medicine - May 16, 2022 Category: Molecular Biology Authors: Sandeep Xxxx Mir Hilal Ahmad Linchi Rani Amal Chandra Mondal Source Type: research