Deregulation of circ_003912 contributes to pathogenesis of erosive oral lichen planus by via sponging microRNA-123, -647 and -31 and upregulating FOXP3
CONCLUSION: In this study, we investigated the molecular mechanisms underlying the pathogenesis of EOLP which involved the functioning of circ_003912. We first demonstrated that circ_003912 was up-regulated in CD4+ T-cells of the EOLP group. And miRNAs including miR-1231, miR-31 and miR-647 were sponged by circ_003912 and down-regulated in CD4+ T cells of the EOLP group, which subsequently up-regulated the expression of FOXP3 and miR-146a, and resulted in the inhibition of NF-kB.PMID:34670484 | DOI:10.1186/s10020-021-00382-4 (Source: Molecular Medicine)
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Zhen Huang Fen Liu Wenjuan Wang Shaobo Ouyang Ting Sang Zikun Huang Lan Liao Jun Wu Source Type: research

The EANM guideline for radiosynoviorthesis
CONCLUSION: These guidelines will assist nuclear medicine physicians in performing radiosynoviorthesis. Since there are differences regarding the radiopharmaceuticals approved for RSO and the official indications between several European countries, this guideline can only give a framework that must be adopted individually.PMID:34671820 | DOI:10.1007/s00259-021-05541-7 (Source: Molecular Medicine)
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: W U Kampen B Boddenberg-P ätzold M Fischer M Gabriel R Klett M Konijnenberg E Kresnik H Lellouche F Paycha L Terslev C Turkmen F van der Zant L Antunovic E Panagiotidis G Gnanasegaran T Kuwert T Van den Wyngaert EANM Bone & Joint Committee, the Dosimetr Source Type: research

microRNAs trip down memory lane
EMBO Mol Med. 2021 Oct 20:e14997. doi: 10.15252/emmm.202114997. Online ahead of print.ABSTRACTA new study by Islam et al, in this issue of EMBO Molecular Medicine, reports three microRNAs in the blood that are linked to inter-individual differences in cognition, prior to any sign of cognitive decline. The novel miRNA biomarkers may assist in predicting the risk of cognitive decline and later of developing dementia and can contribute to decision strategies about early therapeutic interventions.PMID:34672084 | DOI:10.15252/emmm.202114997 (Source: Molecular Medicine)
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Nancy S Yacovzada Eran Hornstein Source Type: research

Uncovering a conserved vulnerability site in SARS-CoV-2 by a human antibody
EMBO Mol Med. 2021 Oct 20:e14544. doi: 10.15252/emmm.202114544. Online ahead of print.ABSTRACTAn essential step for SARS-CoV-2 infection is the attachment to the host cell receptor by its Spike receptor-binding domain (RBD). Most of the existing RBD-targeting neutralizing antibodies block the receptor-binding motif (RBM), a mutable region with the potential to generate neutralization escape mutants. Here, we isolated and structurally characterized a non-RBM-targeting monoclonal antibody (FD20) from convalescent patients. FD20 engages the RBD at an epitope distal to the RBM with a KD of 5.6 nM, neutralizes SARS-CoV-2 includ...
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Tingting Li Hongmin Cai Yapei Zhao Yanfang Li Yanling Lai Hebang Yao Liu Daisy Liu Zhou Sun Martje Fentener van Vlissingen Thijs Kuiken Corine H GeurtsvanKessel Ning Zhang Bingjie Zhou Lu Lu Yuhuan Gong Wenming Qin Moumita Mondal Bowen Duan Shiqi Xu Audre Source Type: research

2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus
This study was aimed to explore the role of glycolysis inhibition by 2-DG on regulating immune responses of OLP-derived T cells. We observed that lactic dehydrogenase A (LDHA) expression was elevated in OLP lesions and local T cells. 2-DG inhibited the expression of LDHA, p-mTOR, Hif1α and PLD2 in T cells; meanwhile, it decreased proliferation and increased apoptosis of T cells. T cells treated by 2-DG showed lower LDHA expression and elevated apoptosis, resulting in a reduced apoptotic population of keratinocytes that were co-cultured with them, which was related to the decreased levels of IFN-γ in co-culture ...
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Fang Wang Jing Zhang Gang Zhou Source Type: research

Contributions of NFKB1 -94insertion/deletion ATTG polymorphism to the susceptibility of gastrointestinal cancers: A meta-analysis
J Cell Mol Med. 2021 Oct 21. doi: 10.1111/jcmm.17004. Online ahead of print.ABSTRACTNuclear factor-kappa B1 (NF-κB1), a pleiotropic transcription factor, functions as a critical contributor to tumorigenesis. Growing numbers of case-control studies were carried out to analyse the potential contribution of NF-κB1 gene variants to gastrointestinal cancer risk, yet remains conflicting conclusions. Therefore, we conducted this most up-to-date meta-analysis to evaluate the relationship between NF-κB1 gene insertion (I)/deletion (D) polymorphism, namely -94ins/delATTG or rs28362491, and the susceptibility to gas...
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Hanqiang Wu Jianrong Liang Source Type: research

A +3 variant at a donor splice site leads to a skipping of the MYH11 exon 32, a recurrent RNA defect causing Heritable Thoracic Aortic Aneurysm and Dissection and/or Patent Ductus Arteriosus
CONCLUSION: This report confirms that among rare variants in MYH11, skipping of exon 32 is recurrent. This finding is of particular interest to establish complex genotype-phenotype correlations where some alleles are associated with autosomal dominant HTAAD/PDA, while others result in recessive or dominant visceral myopathies.PMID:34672437 | DOI:10.1002/mgg3.1814 (Source: Molecular Medicine)
Source: Molecular Medicine - October 21, 2021 Category: Molecular Biology Authors: Bertrand Chesneau Aur élie Plancke Guillaume Rolland Bertrand Marcheix Yves Dulac Thomas Edouard Julie Plaisanci é Marion Aubert-Mucca Sophie Julia Maud Langeois Thierry Lavabre-Bertrand Philippe Khau Van Kien Source Type: research

Microglial activation in the neurodevelopment: A narrative review
Curr Mol Med. 2021 Oct 18. doi: 10.2174/1566524021666211018112757. Online ahead of print.ABSTRACTMicroglia are immune cells found in the central nervous system (CNS) involved in infection combat and cellular debris clean. These glial cells are involved in synaptogenesis during brain development by their interactions with neurons and other glial cells. These relations are associated with the secretion of signaling molecules, such as chemokines and neurotrophic factors. Microglia cells influence synapsis and neuron morphology during different phases of development. Also, other systems, for example, gut microbiota indirectly ...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Airam Barbosa de Moura Morgana Sonza Abitante Ritele Hernandez Silva Jo ão Quevedo Gislaine Zilli R éus Source Type: research

Hsp90 up-regulates PD-L1 to promote HPV-positive cervical cancer via HER2/PI3K/AKT pathway
CONCLUSION: In this study, we demonstrated a promising tumor-suppressive effect of Hsp90 knockdown in HPV16+ cervical cancers, and investigated the underlying molecular pathway. Our results suggested that Hsp90 knockdown holds great therapeutic potential in treating HPV16+ cervical cancers.PMID:34666670 | DOI:10.1186/s10020-021-00384-2 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Jie Zeng Si-Li He Li-Jie Li Chen Wang Source Type: research

Long noncoding RNA MAPKAPK5-AS1 promoted lipopolysaccharide-induced inflammatory damage in the myocardium by sponging microRNA-124-3p/E2F3
CONCLUSION: MAPKAPK5-AS1/miR-124-3p/E2F3 axis regulates LPS-related H9C2 cell apoptosis and inflammatory response.PMID:34666672 | DOI:10.1186/s10020-021-00385-1 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Weiwei Chen Guangyuan Gao Mengjie Yan Ming Yu Kaiyao Shi Ping Yang Source Type: research

Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs
Exp Mol Med. 2021 Oct 19. doi: 10.1038/s12276-021-00688-7. Online ahead of print.ABSTRACTFibrin, one of the components of the extracellular matrix (ECM), acts as a transport barrier within the core of tumors by constricting the blood vessels and forming clots, leading to poor intratumoral distribution of anticancer drugs. Our group previously developed a microplasmin-based thrombolytic ferritin nanocage that efficiently targets and dissolves clots without causing systemic fibrinolysis or disrupting hemostatic clots. We hypothesized that the thrombolytic nanocage-mediated degradation of fibrin clots in the tumor ECM can lea...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Junyoung Seo Jae Do Yoo Minseong Kim Gayong Shim Yu-Kyoung Oh Rang-Woon Park Byungheon Lee In-San Kim Soyoun Kim Source Type: research

CSF circulation regulates depression: do not disturb the flow!
Mol Psychiatry. 2021 Oct 20. doi: 10.1038/s41380-021-01323-7. Online ahead of print.NO ABSTRACTPMID:34667262 | DOI:10.1038/s41380-021-01323-7 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Eduardo Loureiro-Campos Lu ísa Pinto Ana Mendanha Falc ão Source Type: research

CircGFRA1 facilitates the malignant progression of HER-2-positive breast cancer via acting as a sponge of miR-1228 and enhancing AIFM2 expression
J Cell Mol Med. 2021 Oct 20. doi: 10.1111/jcmm.16963. Online ahead of print.ABSTRACTCircRNAs (circular RNA) are reported to regulate onset and progress multiple cancers. Nonetheless, the function along with the underlying mechanisms of circRNAs in HER-2-positive breast cancer (BC) remains unclear. CircRNA microarrays were performed to elucidate expression profiles of HER-2-positive BC cells. circRNA levels were quantified using qRT-PCR assay. Various in vitro along with in vivo assays were employed to further explore the effects of circGFRA1 in the progress of HER-2-positive BC and interactions of circGFRA1, miR-1228 and A...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Meiheban Bazhabayi Xingsheng Qiu Xing Li Anli Yang Wei Wen Xiaoli Zhang Xiangsheng Xiao Rongfang He Peng Liu Source Type: research

Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression
In this study, second-generation high-throughput sequencing revealed a reduction in miR-99a-3p expression in patients with SLE; however, the specific mechanism underlying this phenomenon remains unclear. After transfection with an miR-99a-3p agomir, the proliferation of Ball-1 cells decreased and the levels of their apoptosis increased. The opposite effects were observed in cells transfected with the miR-99a-3p antagomir. Luciferase reporter assay indicated that miR-99a-3p directly targeted EIF4EBP1. Rescue experiments confirmed the proposed interaction between miR-99a-3p and EIF4EBP1. In vitro, in vivo and clinical invest...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Meng Yang Binbin Yang Danqi Deng Source Type: research

Different mutations in the MMUT gene are associated with the effect of vitamin B12 in a cohort of 266 Chinese patients with mut-type methylmalonic acidemia: A retrospective study
Mol Genet Genomic Med. 2021 Oct 20:e1822. doi: 10.1002/mgg3.1822. Online ahead of print.ABSTRACTBACKGROUND: To summarize the relationship between different MMUT gene mutations and the response to vitamin B12 in MMA.METHODS: This was a retrospective study of patients diagnosed with mut-type MMA. All patients with mut-type MMA were tested for responsiveness to vitamin B12.RESULTS: There were 81, 27, and 158 patients in the completely responsive, partially responsive, and nonresponsive groups, respectively, and the proportions of symptom occurrence were 30/81 (37.0%), 21/27 (77.8%), and 131/158 (82.9%), respectively (p A, c.2...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Yue Yu Ruixue Shuai Lili Liang Wenjuan Qiu Linghua Shen Shengnan Wu Haiyan Wei Yongxing Chen Chiju Yang Peng Xu Xigui Chen Hui Zou Jizhen Feng Tingting Niu Haili Hu Jun Ye Huiwen Zhang Deyun Lu Zhuwen Gong Xia Zhan Wenjun Ji Xuefan Gu Lianshu Han Source Type: research

Epidemiology of variant transthyretin amyloidosis at a reference center in Argentina
CONCLUSION: We present the first prevalence report of TTR mutations in a reference center of amyloidosis in Argentina. The most frequent genetic variant was p.Val50Met. Our data show considerable phenotypic heterogeneity in the patients with ATTRv.PMID:34668655 | DOI:10.1002/mgg3.1812 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Maria S Saez Maria A Aguirre Diego P érez de Arenaza Patricia Sorroche Elsa Nucifora Maria L Posadas Martinez Source Type: research

Predictors of empowerment in parents of children with autism and related neurodevelopmental disorders who are undergoing genetic testing
CONCLUSION: The informational needs of families play an important role in their empowerment during genetic testing. Meeting these needs and monitoring empowerment can aid genomic technologies integration in personalized healthcare for ASD/NDD.PMID:34668664 | DOI:10.1002/mgg3.1803 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Iskra Peltekova Afiqah Yusuf Jennifer Frei Tal Savion-Lemieux Ridha Joober Jennifer Howe Stephen W Scherer Mayada Elsabbagh Source Type: research

Correction to: Cross ‑reactive, natural IgG recognizing L. major promote parasite internalization by dendritic cells and promote protective immunity
J Mol Med (Berl). 2021 Oct 20. doi: 10.1007/s00109-021-02156-1. Online ahead of print.NO ABSTRACTPMID:34669004 | DOI:10.1007/s00109-021-02156-1 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Filiz Demircik Susanna Lopez Kostka Stefan Tenzer Ari Waisman Esther Von Stebut Source Type: research

Microglial activation in the neurodevelopment: A narrative review
Curr Mol Med. 2021 Oct 18. doi: 10.2174/1566524021666211018112757. Online ahead of print.ABSTRACTMicroglia are immune cells found in the central nervous system (CNS) involved in infection combat and cellular debris clean. These glial cells are involved in synaptogenesis during brain development by their interactions with neurons and other glial cells. These relations are associated with the secretion of signaling molecules, such as chemokines and neurotrophic factors. Microglia cells influence synapsis and neuron morphology during different phases of development. Also, other systems, for example, gut microbiota indirectly ...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Airam Barbosa de Moura Morgana Sonza Abitante Ritele Hernandez Silva Jo ão Quevedo Gislaine Zilli R éus Source Type: research

Hsp90 up-regulates PD-L1 to promote HPV-positive cervical cancer via HER2/PI3K/AKT pathway
CONCLUSION: In this study, we demonstrated a promising tumor-suppressive effect of Hsp90 knockdown in HPV16+ cervical cancers, and investigated the underlying molecular pathway. Our results suggested that Hsp90 knockdown holds great therapeutic potential in treating HPV16+ cervical cancers.PMID:34666670 | DOI:10.1186/s10020-021-00384-2 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Jie Zeng Si-Li He Li-Jie Li Chen Wang Source Type: research

Long noncoding RNA MAPKAPK5-AS1 promoted lipopolysaccharide-induced inflammatory damage in the myocardium by sponging microRNA-124-3p/E2F3
CONCLUSION: MAPKAPK5-AS1/miR-124-3p/E2F3 axis regulates LPS-related H9C2 cell apoptosis and inflammatory response.PMID:34666672 | DOI:10.1186/s10020-021-00385-1 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Weiwei Chen Guangyuan Gao Mengjie Yan Ming Yu Kaiyao Shi Ping Yang Source Type: research

Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs
Exp Mol Med. 2021 Oct 19. doi: 10.1038/s12276-021-00688-7. Online ahead of print.ABSTRACTFibrin, one of the components of the extracellular matrix (ECM), acts as a transport barrier within the core of tumors by constricting the blood vessels and forming clots, leading to poor intratumoral distribution of anticancer drugs. Our group previously developed a microplasmin-based thrombolytic ferritin nanocage that efficiently targets and dissolves clots without causing systemic fibrinolysis or disrupting hemostatic clots. We hypothesized that the thrombolytic nanocage-mediated degradation of fibrin clots in the tumor ECM can lea...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Junyoung Seo Jae Do Yoo Minseong Kim Gayong Shim Yu-Kyoung Oh Rang-Woon Park Byungheon Lee In-San Kim Soyoun Kim Source Type: research

CSF circulation regulates depression: do not disturb the flow!
Mol Psychiatry. 2021 Oct 20. doi: 10.1038/s41380-021-01323-7. Online ahead of print.NO ABSTRACTPMID:34667262 | DOI:10.1038/s41380-021-01323-7 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Eduardo Loureiro-Campos Lu ísa Pinto Ana Mendanha Falc ão Source Type: research

CircGFRA1 facilitates the malignant progression of HER-2-positive breast cancer via acting as a sponge of miR-1228 and enhancing AIFM2 expression
J Cell Mol Med. 2021 Oct 20. doi: 10.1111/jcmm.16963. Online ahead of print.ABSTRACTCircRNAs (circular RNA) are reported to regulate onset and progress multiple cancers. Nonetheless, the function along with the underlying mechanisms of circRNAs in HER-2-positive breast cancer (BC) remains unclear. CircRNA microarrays were performed to elucidate expression profiles of HER-2-positive BC cells. circRNA levels were quantified using qRT-PCR assay. Various in vitro along with in vivo assays were employed to further explore the effects of circGFRA1 in the progress of HER-2-positive BC and interactions of circGFRA1, miR-1228 and A...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Meiheban Bazhabayi Xingsheng Qiu Xing Li Anli Yang Wei Wen Xiaoli Zhang Xiangsheng Xiao Rongfang He Peng Liu Source Type: research

Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression
In this study, second-generation high-throughput sequencing revealed a reduction in miR-99a-3p expression in patients with SLE; however, the specific mechanism underlying this phenomenon remains unclear. After transfection with an miR-99a-3p agomir, the proliferation of Ball-1 cells decreased and the levels of their apoptosis increased. The opposite effects were observed in cells transfected with the miR-99a-3p antagomir. Luciferase reporter assay indicated that miR-99a-3p directly targeted EIF4EBP1. Rescue experiments confirmed the proposed interaction between miR-99a-3p and EIF4EBP1. In vitro, in vivo and clinical invest...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Meng Yang Binbin Yang Danqi Deng Source Type: research

Different mutations in the MMUT gene are associated with the effect of vitamin B12 in a cohort of 266 Chinese patients with mut-type methylmalonic acidemia: A retrospective study
Mol Genet Genomic Med. 2021 Oct 20:e1822. doi: 10.1002/mgg3.1822. Online ahead of print.ABSTRACTBACKGROUND: To summarize the relationship between different MMUT gene mutations and the response to vitamin B12 in MMA.METHODS: This was a retrospective study of patients diagnosed with mut-type MMA. All patients with mut-type MMA were tested for responsiveness to vitamin B12.RESULTS: There were 81, 27, and 158 patients in the completely responsive, partially responsive, and nonresponsive groups, respectively, and the proportions of symptom occurrence were 30/81 (37.0%), 21/27 (77.8%), and 131/158 (82.9%), respectively (p A, c.2...
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Yue Yu Ruixue Shuai Lili Liang Wenjuan Qiu Linghua Shen Shengnan Wu Haiyan Wei Yongxing Chen Chiju Yang Peng Xu Xigui Chen Hui Zou Jizhen Feng Tingting Niu Haili Hu Jun Ye Huiwen Zhang Deyun Lu Zhuwen Gong Xia Zhan Wenjun Ji Xuefan Gu Lianshu Han Source Type: research

Epidemiology of variant transthyretin amyloidosis at a reference center in Argentina
CONCLUSION: We present the first prevalence report of TTR mutations in a reference center of amyloidosis in Argentina. The most frequent genetic variant was p.Val50Met. Our data show considerable phenotypic heterogeneity in the patients with ATTRv.PMID:34668655 | DOI:10.1002/mgg3.1812 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Maria S Saez Maria A Aguirre Diego P érez de Arenaza Patricia Sorroche Elsa Nucifora Maria L Posadas Martinez Source Type: research

Predictors of empowerment in parents of children with autism and related neurodevelopmental disorders who are undergoing genetic testing
CONCLUSION: The informational needs of families play an important role in their empowerment during genetic testing. Meeting these needs and monitoring empowerment can aid genomic technologies integration in personalized healthcare for ASD/NDD.PMID:34668664 | DOI:10.1002/mgg3.1803 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Iskra Peltekova Afiqah Yusuf Jennifer Frei Tal Savion-Lemieux Ridha Joober Jennifer Howe Stephen W Scherer Mayada Elsabbagh Source Type: research

Correction to: Cross ‑reactive, natural IgG recognizing L. major promote parasite internalization by dendritic cells and promote protective immunity
J Mol Med (Berl). 2021 Oct 20. doi: 10.1007/s00109-021-02156-1. Online ahead of print.NO ABSTRACTPMID:34669004 | DOI:10.1007/s00109-021-02156-1 (Source: Molecular Medicine)
Source: Molecular Medicine - October 20, 2021 Category: Molecular Biology Authors: Filiz Demircik Susanna Lopez Kostka Stefan Tenzer Ari Waisman Esther Von Stebut Source Type: research

CXCL10 levels at hospital admission predict COVID-19 outcome: hierarchical assessment of 53 putative inflammatory biomarkers in an observational study
CONCLUSIONS: CXCL10 results from a data-driven analysis, that accounts for presence of confounding factors, as the most robust predictive biomarker of patient outcome in COVID-19.PMID:34663207 | DOI:10.1186/s10020-021-00390-4 (Source: Molecular Medicine)
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Nicola I Lor è Rebecca De Lorenzo Paola M V Rancoita Federica Cugnata Alessandra Agresti Francesco Benedetti Marco E Bianchi Chiara Bonini Annalisa Capobianco Caterina Conte Angelo Corti Roberto Furlan Paola Mantegani Norma Maugeri Clara Sciorati Fabio S Source Type: research

Determination of the segmental hepatic clearance rate of < sup > 99m < /sup > Tc-mebrofenin and its application in the functional assessment of future liver remnant after liver resection
Rev Esp Med Nucl Imagen Mol (Engl Ed). 2021 Oct 15:S2253-8089(21)00135-X. doi: 10.1016/j.remnie.2021.09.001. Online ahead of print.ABSTRACT99mTc-mebrofenin hepatobiliary scintigraphy with SPECT/CT (HBS-M) has become an important quantitative method to evaluate global liver function and future liver remnant (FLR) function in patients who are candidates for resective liver surgery. The purpose of this work was to describe the method in the prediction of post-surgical liver failure. The overall liver function and that of the FLR are obtained by analysis of the initial dynamic phase of the scan. Liver volume to be preserved is...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Jon Andoni U ña-Gorospe Source Type: research

Plasmablastic lymphoma of the oral cavity in an HIV-positive patient: Assessment by < sup > 18 < /sup > F FDG PET/CT
Rev Esp Med Nucl Imagen Mol (Engl Ed). 2021 Oct 15:S2253-8089(21)00137-3. doi: 10.1016/j.remnie.2021.09.003. Online ahead of print.NO ABSTRACTPMID:34663556 | DOI:10.1016/j.remnie.2021.09.003 (Source: Molecular Medicine)
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Yueli Tian Yuepeng Zhang Yong He Bin Wen Chongjiao Li Source Type: research

Methods in organoids: a model that goes beyond our imagination
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00685-w. Online ahead of print.NO ABSTRACTPMID:34663934 | DOI:10.1038/s12276-021-00685-w (Source: Molecular Medicine)
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Bon-Kyoung Koo Source Type: research

Biobanking of human gut organoids for translational research
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00606-x. Online ahead of print.ABSTRACTThe development of human organoid culture models has led to unprecedented opportunities to generate self-organizing, three-dimensional miniature organs that closely mimic in vivo conditions. The ability to expand, culture, and bank such organoids now provide researchers with the opportunity to generate next-generation living biobanks, which will substantially contribute to translational research in a wide range of areas, including drug discovery and testing, regenerative medicine as well as the development of a personalized treatment a...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Francesca Perrone Matthias Zilbauer Source Type: research

Organoids as host models for infection biology - a review of methods
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00629-4. Online ahead of print.ABSTRACTInfectious diseases are a major threat worldwide. With the alarming rise of antimicrobial resistance and emergence of new potential pathogens, a better understanding of the infection process is urgently needed. Over the last century, the development of in vitro and in vivo models has led to remarkable contributions to the current knowledge in the field of infection biology. However, applying recent advances in organoid culture technology to research infectious diseases is now taking the field to a higher level of complexity. Here, we d...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Carmen Aguilar Marta Alves da Silva Margarida Saraiva Mastura Neyazi I Anna S Olsson Sina Bartfeld Source Type: research

Strategies for genetic manipulation of adult stem cell-derived organoids
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00609-8. Online ahead of print.ABSTRACTOrganoid technology allows the expansion of primary epithelial cells from normal and diseased tissues, providing a unique model for human (patho)biology. In a three-dimensional environment, adult stem cells self-organize and differentiate to gain tissue-specific features. Accessibility to genetic manipulation enables the investigation of the molecular mechanisms underlying cell fate regulation, cell differentiation and cell interactions. In recent years, powerful methodologies using lentiviral transgenesis, CRISPR/Cas9 gene editing, an...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Constantin Menche Henner F Farin Source Type: research

Organoids in image-based phenotypic chemical screens
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00641-8. Online ahead of print.ABSTRACTImage-based phenotypic screening relies on the extraction of multivariate information from cells cultured under a large variety of conditions. Technical advances in high-throughput microscopy enable screening in increasingly complex and biologically relevant model systems. To this end, organoids hold great potential for high-content screening because they recapitulate many aspects of parent tissues and can be derived from patient material. However, screening is substantially more difficult in organoids than in classical cell lines from...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Ilya Lukonin Marietta Zinner Prisca Liberali Source Type: research

Gastrointestinal cancer organoids-applications in basic and translational cancer research
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00654-3. Online ahead of print.ABSTRACTCancer is a major health problem and a leading cause of death worldwide. Early cancer detection and continuous changes in treatment strategies have improved overall patient survival. The recent development of targeted drugs offers new opportunities for personalized cancer treatment. Nevertheless, individualized treatment is accompanied by the need for biomarkers predicting the response of a patient to a certain drug. One of the most promising breakthroughs in recent years that might help to overcome this problem is the organoid technol...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Therese Seidlitz Daniel E Stange Source Type: research

Dissecting single-cell genomes through the clonal organoid technique
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00680-1. Online ahead of print.ABSTRACTThe revolution in genome sequencing technologies has enabled the comprehensive detection of genomic variations in human cells, including inherited germline polymorphisms, de novo mutations, and postzygotic mutations. When these technologies are combined with techniques for isolating and expanding single-cell DNA, the landscape of somatic mosaicism in an individual body can be systematically revealed at a single-cell resolution. Here, we summarize three strategies (whole-genome amplification, microdissection of clonal patches in the tis...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Jeonghwan Youk Hyun Woo Kwon Ryul Kim Young Seok Ju Source Type: research

Hepatocyte organoids and cell transplantation: What the future holds
Exp Mol Med. 2021 Oct 18. doi: 10.1038/s12276-021-00579-x. Online ahead of print.ABSTRACTHistorically, primary hepatocytes have been difficult to expand or maintain in vitro. In this review, we will focus on recent advances in establishing hepatocyte organoids and their potential applications in regenerative medicine. First, we provide a background on the renewal of hepatocytes in the homeostatic as well as the injured liver. Next, we describe strategies for establishing primary hepatocyte organoids derived from either adult or fetal liver based on insights from signaling pathways regulating hepatocyte renewal in vivo. The...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Weng Chuan Peng Lianne J Kraaier Thomas A Kluiver Source Type: research

Differential role of residual metabolic tumor volume in inoperable stage III NSCLC after chemoradiotherapy  ± immune checkpoint inhibition
CONCLUSION: In stage III NSCLC, smaller rMTV is highly associated with superior clinical outcome, especially in patients undergoing CRT without ICI. Patients with CRT-IO show significantly improved outcome compared to CRT patients. Of note, clinical outcome in CRT-IO patients is independent of residual MTV. Hence, even patients with large rMTV might profit from ICI despite extensive tumor load.PMID:34664091 | DOI:10.1007/s00259-021-05584-w (Source: Molecular Medicine)
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Marcus Unterrainer Julian Taugner Lukas K äsmann Amanda Tufman Niels Reinmuth Minglun Li Lena M Mittlmeier Peter Bartenstein Wolfgang G Kunz Jens Ricke Claus Belka Chukwuka Eze Farkhad Manapov Source Type: research

Prognostic factors in children and adolescents with differentiated thyroid carcinoma treated with total thyroidectomy and RAI: a real-life multicentric study
Eur J Nucl Med Mol Imaging. 2021 Oct 19. doi: 10.1007/s00259-021-05586-8. Online ahead of print.ABSTRACTPURPOSE: This multicentric study aimed to investigate the main prognostic factors associated with treatment response at 1 year after radioactive iodine therapy (RAIT) and the last disease status in pediatric patients affected by differentiated thyroid carcinoma (DTC).MATERIALS AND METHODS: In the period 1990-2020, all consecutive patients ≤ 18 years from six different centers were retrospectively included. Patients were classified as low, intermediate, and high risk for persistence/recurrence. The response to RAIT was...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Angelina Cistaro Natale Quartuccio Maria Carmen Garganese Maria Felicia Villani Claudio Altini Milena Pizzoferro Arnoldo Piccardo Manlio Cabria Michela Massollo Mohamad Maghnie Alfredo Campenn ì Massimiliano Siracusa Sergio Baldari Stefano Panareo Luca U Source Type: research

Niclosamide for Covid-19: bridging the gap
CONCLUSIONS: NCL has anti-inflammatory and immune regulatory effects by modulating the release of pro-inflammatory cytokines, inhibition of NF-κB /NLRP3 inflammasome and mTOR signaling pathway. NCL has an anti-SARS-CoV-2 effect via interruption of viral life-cycle and/or induction of cytopathic effect. Prospective clinical studies and clinical trials are mandatory to confirm the potential role of NCL in patients with Covid-19 concerning the severity and clinical outcomes.PMID:34664162 | DOI:10.1007/s11033-021-06770-7 (Source: Molecular Medicine)
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Hayder M Al-Kuraishy Ali I Al-Gareeb Khalid J Alzahrani Athanasios Alexiou Gaber El-Saber Batiha Source Type: research

Integrative computational immunogenomic profiling of cortisol-secreting adrenocortical carcinoma
In this study, we characterized the TME of ACC patients and compared the immunogenomic profiles of nonCS-ACC and CS-ACC tumours to assess the impact of differentially expressed genes (DEGs) by utilizing The Cancer Genome Atlas (TCGA) database. Immunogenomic comparison (CS- vs. nonCS-ACC tumour TMEs) demonstrated an immunosuppressive expression profile with a direct impact on patient survival. We identified several primary prognostic indicators and potential targets within ACC tumour immune landscape. Differentially expressed immune genes with prognostic significance provide additional insight into the understanding of pote...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Jordan J Baechle David N Hanna Konjeti R Sekhar Jeffrey C Rathmell W Kimryn Rathmell Naira Baregamian Source Type: research

YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosis
In this study, a combination of shRNA-mediated synthetic lethality screening and transcriptomic analysis revealed the transcription factors YAP/TAZ as key drivers of Sorafenib resistance in hepatocellular carcinoma (HCC) by repressing Sorafenib-induced ferroptosis. Mechanistically, in a TEAD-dependent manner, YAP/TAZ induce the expression of SLC7A11, a key transporter maintaining intracellular glutathione homeostasis, thus enabling HCC cells to overcome Sorafenib-induced ferroptosis. At the same time, YAP/TAZ sustain the protein stability, nuclear localization, and transcriptional activity of ATF4 which in turn cooperates ...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Ruize Gao Ravi K R Kalathur Mairene Coto-Llerena Caner Ercan David Buechel Song Shuang Salvatore Piscuoglio Michael T Dill Fernando D Camargo Gerhard Christofori Fengyuan Tang Source Type: research

Dexmedetomidine alleviates hepatic ischaemia-reperfusion injury via the PI3K/AKT/Nrf2-NLRP3 pathway
This study investigated protective mechanism of Dex in hepatic I/R injury. The human hepatocyte line L02 received hypoxia/reoxygenation (H/R) treatment to stimulate cell model of hepatic I/R. The levels of pyroptosis proteins and inflammatory factors were detected. Functional rescue experiments were performed to confirm the effects of miR-494 and JUND on hepatic I/R injury. The levels of JUND, PI3K/p-PI3K, AKT/p-AKT, Nrf2, and NLRP3 activation were detected. The rat model of hepatic I/R injury was established to confirm the effect of Dex in vivo. Dex reduced pyroptosis and inflammation in H/R cells. Dex increased miR-494 e...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Yan Wu Gaolin Qiu Hainie Zhang Leilei Zhu Gao Cheng Yiqiao Wang Yuanhai Li Weiwei Wu Source Type: research

Current treatment strategies for COVID ‑19 (Review)
Mol Med Rep. 2021 Dec;24(6):858. doi: 10.3892/mmr.2021.12498. Epub 2021 Oct 19.ABSTRACTThe spread of the novel severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) emerged suddenly at the end of 2019 and the disease came to be known as coronavirus disease 2019 (COVID‑19). To date, there is no specific therapy established to treat COVID‑19. Identifying effective treatments is urgently required to treat patients and stop the transmission of SARS‑CoV‑2 in humans. For the present review,>100 publications on therapeutic agents for COVID‑19, including in vitro and in vivo animal studies, case reports, re...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Fabin Han Yanming Liu Mei Mo Juanli Chen Cheng Wang Yong Yang Jibiao Wu Source Type: research

Peroxisome proliferator ‑activated receptor β/δ regulates cerebral vasospasm after subarachnoid hemorrhage via modulating vascular smooth muscle cells phenotypic conversion
Mol Med Rep. 2021 Dec;24(6):860. doi: 10.3892/mmr.2021.12500. Epub 2021 Oct 19.ABSTRACTCerebral vasospasm (CVS) is a common complication of subarachnoid hemorrhage (SAH) with high deformity rates and cerebral vascular smooth muscle cells (VSMCs) phenotypic switch is considered to be involved in the regulation of CVS. However, to the best of the authors' knowledge, its underlying molecular mechanism remains to be elucidated. Peroxisome proliferator‑activated receptor β/δ (PPARβ/δ) has been demonstrated to be involved in the modulation of vascular cells proliferation and maintains the autoregulation f...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Li Xu Jiang Wu Yuan Liu Gang Chen Chao Ma Hongrong Zhang Source Type: research

Molecular and cellular mechanisms of spastin in  neural development and disease (Review)
Int J Mol Med. 2021 Dec;48(6):218. doi: 10.3892/ijmm.2021.5051. Epub 2021 Oct 19.ABSTRACTSpastin is a microtubule (MT)‑severing enzyme identified from mutations of hereditary spastic paraplegia in 1999 and extensive studies indicate its vital role in various cellular activities. In the past two decades, efforts have been made to understand the underlying molecular mechanisms of how spastin is linked to neural development and disease. Recent studies on spastin have unraveled the mechanistic processes of its MT‑severing activity and revealed that spastin acts as an MT amplifier to mediate its remodeling, thus providing v...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Qiuling Liu Guowei Zhang Zhisheng Ji Hongsheng Lin Source Type: research

Gomisin M2 alleviates psoriasis ‑like skin inflammation by inhibiting inflammatory signaling pathways
Mol Med Rep. 2021 Dec;24(6):859. doi: 10.3892/mmr.2021.12499. Epub 2021 Oct 19.ABSTRACTPsoriasis, a chronic inflammatory skin disease, is characterized by the excessive proliferation and impaired differentiation of epidermal keratinocytes and is accompanied by the increased infiltration of inflammatory cells. The condition requires long‑term treatment and has no definitive cure. Hence, supplements and therapeutic agents have been intensely investigated. Gomisin M2 (GM2), a lignan extracted from Schisandra chinensis (Turcz). Baill. (Schisandraceae; S. chinensis), has demonstrated diverse pharmacological properties, includ...
Source: Molecular Medicine - October 19, 2021 Category: Molecular Biology Authors: Namkyung Kim Soyoung Lee Jinjoo Kang Taeg Kyu Kwon Dongwoo Khang Sang-Hyun Kim Source Type: research