Hyperbaric oxygen therapy effectively alleviates D-galactose-induced-age-related cardiac dysfunction via attenuating mitochondrial dysfunction in pre-diabetic rats
Aging (Albany NY). 2021 Apr 16;13. doi: 10.18632/aging.202970. Online ahead of print.ABSTRACTCurrently, the prevalence of obesity in aging populations is fast growing worldwide. Aging induced by D-galactose (D-gal) is proven to cause the worsening of cardiac dysfunction in pre-diabetic rats via deteriorating cardiac mitochondrial function. Hyperbaric oxygen therapy (HBOT) has been shown to attenuate D-gal-induced cognitive deterioration through decreased inflammation and apoptosis. We tested the hypothesis that HBOT alleviates D-gal induced cardiac dysfunction via improving mitochondrial function in pre-diabetic rats. Wist...
Source: Aging - April 16, 2021 Category: Biomedical Science Authors: Cherry Bo-Htay Thazin Shwe Thidarat Jaiwongkam Sasiwan Kerdphoo Wasana Pratchayasakul Thienchai Pattarasakulchai Krekwit Shinlapawittayatorn Siriporn C Chattipakorn Nipon Chattipakorn Source Type: research

Association between white matter hyperintensity load and grey matter atrophy in mild cognitive impairment is not unidirectional
We examined the load-dependent influence of WMH on GMV and cognition in 183 subjects. T1-MRI data from 93 Mild Cognitive Impairment (MCI) and 90 cognitively normal subjects were studied and WMH load was categorized into low, medium and high terciles. We examined how differing loads of WMH related to whole-brain voxel-wise and regional DMN and ECN GMV. We further investigated how regional GMV moderated the relationship between WMH and cognition. We found differential load-dependent effects of WMH burden on voxel-wise and regional atrophy in only MCI. At high load, as expected WMH negatively related to both ECN and DMN GMV, ...
Source: Aging - April 16, 2021 Category: Biomedical Science Authors: Ashwati Vipin Benjamin Yi Xin Wong Dilip Kumar Audrey Low Kok Pin Ng Nagaendran Kandiah Source Type: research

A novel focal adhesion related gene signature for prognostic prediction in hepatocellular carcinoma
In this study, RNA expression profiles were downloaded from public databases. A five focal adhesion related gene signature model was established by the least absolute shrinkage and selection operator Cox regression analysis, which categorized patients into high- and low-risk groups. Multivariate Cox regression analysis showed that the risk score was an independent predictor for overall survival. Single-sample gene set enrichment analysis revealed that immune status was different between the two risk groups, and tumor-related pathways were enriched in high-risk group. The risk score was significantly associated with tumor g...
Source: Aging - April 14, 2021 Category: Biomedical Science Authors: Zhuo Lin Dan Miao Qian Xu Xiaodong Wang Fujun Yu Source Type: research

A novel focal adhesion related gene signature for prognostic prediction in hepatocellular carcinoma
In this study, RNA expression profiles were downloaded from public databases. A five focal adhesion related gene signature model was established by the least absolute shrinkage and selection operator Cox regression analysis, which categorized patients into high- and low-risk groups. Multivariate Cox regression analysis showed that the risk score was an independent predictor for overall survival. Single-sample gene set enrichment analysis revealed that immune status was different between the two risk groups, and tumor-related pathways were enriched in high-risk group. The risk score was significantly associated with tumor g...
Source: Aging - April 14, 2021 Category: Biomedical Science Authors: Zhuo Lin Dan Miao Qian Xu Xiaodong Wang Fujun Yu Source Type: research

A novel focal adhesion related gene signature for prognostic prediction in hepatocellular carcinoma
In this study, RNA expression profiles were downloaded from public databases. A five focal adhesion related gene signature model was established by the least absolute shrinkage and selection operator Cox regression analysis, which categorized patients into high- and low-risk groups. Multivariate Cox regression analysis showed that the risk score was an independent predictor for overall survival. Single-sample gene set enrichment analysis revealed that immune status was different between the two risk groups, and tumor-related pathways were enriched in high-risk group. The risk score was significantly associated with tumor g...
Source: Aging - April 14, 2021 Category: Biomedical Science Authors: Zhuo Lin Dan Miao Qian Xu Xiaodong Wang Fujun Yu Source Type: research

VOCs profile can discriminate biological age
Aging (Albany NY). 2021 Apr 11. doi: 10.18632/aging.202959. Online ahead of print.NO ABSTRACTPMID:33846279 | DOI:10.18632/aging.202959 (Source: Aging)
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Maria Conte Giuseppe Conte Stefano Salvioli Source Type: research

Multiomics integrative analysis identifies < em > APOE < /em > allele-specific blood biomarkers associated to Alzheimer's disease etiopathogenesis
Aging (Albany NY). 2021 Apr 12;13. doi: 10.18632/aging.202950. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is the most common form of dementia, currently affecting 35 million people worldwide. Apolipoprotein E (APOE) ε4 allele is the major risk factor for sporadic, late-onset AD (LOAD), which comprises over 95% of AD cases, increasing the risk of AD 4-12 fold. Despite this, the role of APOE in AD pathogenesis is still a mystery. Aiming for a better understanding of APOE-specific effects, the ADAPTED consortium analysed and integrated publicly available data of multiple OMICS technologies from both plasma...
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Laura Madrid Sonia Moreno-Grau Shahzad Ahmad Antonio Gonz ález-Pérez Itziar de Rojas Rui Xia Pamela V Martino Adami Pablo Garc ía-González Luca Kleineidam Qiong Yang Vincent Damotte Joshua C Bis Fuensanta Noguera-Perea C éline Bellenguez Xueqiu Jian Source Type: research

Aging diabetes, deconstructing the cerebrovascular wall
Aging (Albany NY). 2021 Apr 12. doi: 10.18632/aging.202963. Online ahead of print.NO ABSTRACTPMID:33846281 | DOI:10.18632/aging.202963 (Source: Aging)
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Fan Fan George W Booz Richard J Roman Source Type: research

Alterations in fecal short chain fatty acids (SCFAs) and branched short-chain fatty acids (BCFAs) in men with benign prostatic hyperplasia (BPH) and metabolic syndrome (MetS)
Aging (Albany NY). 2021 Apr 13;13. doi: 10.18632/aging.202968. Online ahead of print.ABSTRACTGut microbiome-derived short-chain fatty acids (SCFAs) emerge in the process of fermentation of polysaccharides that resist digestion (dietary fiber, resistant starch). SCFAs have a very high immunomodulatory potential and ensure local homeostasis of the intestinal epithelium, which helps maintain the intestinal barrier. We analyzed the association between stool SCFAs levels acetic acid (C 2:0), propionic acid (C 3:0), isobutyric acid (C 4:0i), butyric acid (C 4:0n), isovaleric acid (C 5:0i) valeric acid (C 5:0n), isocaproic acid (...
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Weronika Ratajczak Arnold Mizerski Aleksandra Ry ł Marcin S łojewski Olimpia Sipak Ma łgorzata Piasecka Maria Laszczy ńska Source Type: research

Potential role of cannabidiol in Parkinson's disease by targeting the WNT/ β-catenin pathway, oxidative stress and inflammation
Aging (Albany NY). 2021 Apr 13;13. doi: 10.18632/aging.202951. Online ahead of print.ABSTRACTParkinson's disease (PD) is a major neurodegenerative disease (ND), presenting a progressive degeneration of the nervous system characterized by a loss of dopamine in the substantia nigra pars compacta. Recent findings have shown that oxidative stress and inflammation play key roles in the development of PD. However, therapies remain uncertain and research for new treatment is of the utmost importance. This review focuses on the potential effects of using cannabidiol (CBD) as a potential therapeutic strategy for the treatment of PD...
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Alexandre Vall ée Jean-No ël Vallée Yves Lecarpentier Source Type: research

LncRNA PlncRNA-1 accelerates the progression of prostate cancer by regulating PTEN/Akt axis
This study aimed to explore the role of PlncRNA-1 in the progression of prostate cancer (PCa). We found that PlncRNA-1 was up-regulated in 85.29% of PCa tissues and could predict the T stage of PCa patients to a certain extent. Results showed that inhibition of PlncRNA-1 expression potentially promoted cell apoptosis, suppressed the proliferation, migration, and invasion of cells, and triggered G2/M cycle arrest in vitro and in vivo. PlncRNA-1 was mainly localized in the nucleus and PlncRNA-1 expression and phosphatase and tensin homologue (PTEN) expression were negatively correlated. Mechanistically, knockdown of PlncRNA-...
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Zilian Cui Hui Gao Ning Yan Yun Dai Hanbo Wang Muwen Wang Jin Wang Dong Zhang Peng Sun Taiguo Qi Qiang Wang Weiting Kang Xunbo Jin Source Type: research

Dual activation of Hedgehog and Wnt/ β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
Aging (Albany NY). 2021 Apr 12;13. doi: 10.18632/aging.202895. Online ahead of print.ABSTRACTMounting evidence has shown that miRNA-150 expression is upregulated in gastric cancer (GC) and is associated with gastric carcinogenesis, but the underlying oncogenic mechanism remains elusive. Here, we discovered that miRNA-150 targets the tumor suppressor SUFU to promote cell proliferation, migration, and the epithelial-mesenchymal transition (EMT) via the dual activation of Hedgehog (Hh) and Wnt signaling. MiRNA-150 was highly expressed in GC tissues and cell lines, and the level of this miRNA was negatively related to that of ...
Source: Aging - April 13, 2021 Category: Biomedical Science Authors: Yin Peng Xiaojing Zhang Huijuan Lin Shiqi Deng Ying Qin Jieqiong He Fan Hu Xiaohui Zhu Xianling Feng Jian Wang Yanjie Wei Xinmin Fan Huan Lin Hassan Ashktorab Duane Smoot Yansi Lv Song Li Stephen J Meltzer Zhe Jin Source Type: research

Potential reversal of epigenetic age using a diet and lifestyle intervention: a pilot randomized clinical trial
Aging (Albany NY). 2021 Apr 12;13. doi: 10.18632/aging.202913. Online ahead of print.ABSTRACTManipulations to slow biological aging and extend healthspan are of interest given the societal and healthcare costs of our aging population. Herein we report on a randomized controlled clinical trial conducted among 43 healthy adult males between the ages of 50-72. The 8-week treatment program included diet, sleep, exercise and relaxation guidance, and supplemental probiotics and phytonutrients. The control group received no intervention. Genome-wide DNA methylation analysis was conducted on saliva samples using the Illumina Methy...
Source: Aging - April 12, 2021 Category: Biomedical Science Authors: Kara N Fitzgerald Romilly Hodges Douglas Hanes Emily Stack David Cheishvili Moshe Szyf Janine Henkel Melissa W Twedt Despina Giannopoulou Josette Herdell Sally Logan Ryan Bradley Source Type: research

Potential reversal of epigenetic age using a diet and lifestyle intervention: a pilot randomized clinical trial
Aging (Albany NY). 2021 Apr 12;13. doi: 10.18632/aging.202913. Online ahead of print.ABSTRACTManipulations to slow biological aging and extend healthspan are of interest given the societal and healthcare costs of our aging population. Herein we report on a randomized controlled clinical trial conducted among 43 healthy adult males between the ages of 50-72. The 8-week treatment program included diet, sleep, exercise and relaxation guidance, and supplemental probiotics and phytonutrients. The control group received no intervention. Genome-wide DNA methylation analysis was conducted on saliva samples using the Illumina Methy...
Source: Aging - April 12, 2021 Category: Biomedical Science Authors: Kara N Fitzgerald Romilly Hodges Douglas Hanes Emily Stack David Cheishvili Moshe Szyf Janine Henkel Melissa W Twedt Despina Giannopoulou Josette Herdell Sally Logan Ryan Bradley Source Type: research

Melatonin attenuates smoking-induced atherosclerosis by activating the Nrf2 pathway via NLRP3 inflammasomes in endothelial cells
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202829. Online ahead of print.ABSTRACTSubstantial evidence suggests that the effects of smoking in atherosclerosis are associated with inflammation mediated by endothelial cells. However, the mechanisms and potential drug therapies for smoking-induced atherosclerosis remain to be clarified. Considering that melatonin exerts beneficial effects in cardiovascular diseases, we examined its effects on cigarette smoke-induced vascular injury. We found that cigarette smoke extract (CSE) treatment induced NLRP3-related pyroptosis in human aortic endothelial cells (HAECs). CSE a...
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Zhewei Zhao Xuebin Wang Rui Zhang Baitao Ma Shuai Niu Xiao Di Leng Ni Changwei Liu Source Type: research

Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation
This study characterizes lncRNA expression profiles in human hyaline chondrocyte dedifferentiation, thereby identifying new potential mechanisms of chondrocyte dedifferentiation worthy of further investigation.PMID:33839696 | DOI:10.18632/aging.202833 (Source: Aging)
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Lin Chen Jinying Xu Shuang Lv Yan Zhao Dongjie Sun Yangyang Zheng Xianglan Li Lihong Zhang Guangfan Chi Yulin Li Source Type: research

LCZ696 ameliorates lipopolysaccharide-induced endothelial injury
Aging (Albany NY). 2021 Apr 11. doi: 10.18632/aging.202692. Online ahead of print.ABSTRACTLipopolysaccharide (LPS)-induced endothelial dysfunction plays an important role in the pathogenesis of cardiovascular diseases. LCZ696, the dual-acting angiotensin receptor blocker, and neprilysin inhibitor has been used for the treatment of heart failure with reduced ejection fraction. Recent work suggests that LCZ696 therapy might have an anti-inflammatory effect in cardiovascular tissue. In the current study, we show that LCZ696 attenuates LPS-induced oxidative stress by reducing the production of intracellular reactive oxygen spe...
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Aihong Gao Yu Wang Xiao Gao Wei Tian Source Type: research

Investigating the mechanisms of Modified Xiaoyaosan (tiaogan-liqi prescription) in suppressing the progression of atherosclerosis, by means of integrative pharmacology and experimental validation
This study identified a list of key targets of TGLQ in the treatment of AS by applying an integrative pharmacology approach, which was validated by in vivo and in vitro experimentation.PMID:33839698 | DOI:10.18632/aging.202832 (Source: Aging)
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Mingtai Chen Yong Luo Ling Men Bo Lin Haidan Lin Ying Li Guofu Zhong Xiaoling Zhong Wenjun Fu Hua Zhou Guangdong Tong Qiang Liu Jienan Luan Source Type: research

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF- κB axis
CONCLUSION: Inhibition of HOXA11-AS protects mice against PD through repressing neuroinflammation and neuronal apoptosis through miR-124-3p-FSTL1-NF-κB axis.PMID:33839699 | DOI:10.18632/aging.202837 (Source: Aging)
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Hua Cao Xinsheng Han Yonglin Jia Baohua Zhang Source Type: research

Agomelatine prevents macrophage infiltration and brain endothelial cell damage in a stroke mouse model
CONCLUSION: Agomelatine prevents macrophage infiltration and brain endothelial cell damage in a stroke mouse model.PMID:33839700 | DOI:10.18632/aging.202836 (Source: Aging)
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Yiqiang Cao Fei Wang Yonggang Wang Jiang Long Source Type: research

Seven interferon gamma response genes serve as a prognostic risk signature that correlates with immune infiltration in lung adenocarcinoma
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202831. Online ahead of print.ABSTRACTInterferon-gamma (IFN-γ) plays a complex role in modulating tumor microenvironment during lung adenocarcinoma (LUAD) development. In order to define the role of IFN-γ response genes in LUAD progression, we characterized the gene expression, mutation profile, protein-protein interaction of 24 IFN-γ response genes, which exhibited significant hazard ratio in overall survival. Two subgroups of LUAD from the TCGA cohort, which showed significant difference in the survival rate, were identified based on the expression o...
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Boyang Yao Lixin Wang Heyong Wang Jinxia Bao Qiwen Li Fengzhi Yu Wenjing Zhu Li Zhang Wang Li Zhan Gu Ke Fei Peng Zhang Fan Zhang Xiaoying Huang Source Type: research

NET1 promotes HCC growth and metastasis < em > in vitro < /em > and < em > in vivo < /em > via activating the Akt signaling pathway
In this study, we found that NET1 expression was upregulated in HCC, and that upregulated NET1 expression was closely associated with poor prognosis and some clinical characteristics in HCC patients. Whilst forced expression of NET1 in HCC cells was observed to significantly promote cell growth and metastasis in vitro and in vivo; downregulation of NET1 was shown to exhibit an opposite inhibitory effect. RNA-seq analysis and gene set enrichment analysis demonstrated that knockdown of NET1 significantly suppressed the level of Akt phosphorylation level and the expression of Akt downstream genes in HCC cells. Moreover, MK220...
Source: Aging - April 11, 2021 Category: Biomedical Science Authors: Zhen-Hua Chen Qian-Zhi Ni Xiu-Ping Zhang Ning Ma Jing-Kai Feng Kang Wang Jing-Jing Li Dong Xie Xing-Yuan Ma Shu-Qun Cheng Source Type: research

COX-2 promotes the osteogenic potential of BMP9 through TGF- β1/p38 signaling in mesenchymal stem cells
This study investigated the effects of transforming growth factor-β1 (TGF-β1) and cyclooxygenase-2 (COX-2) on bone morphogenetic protein 9 (BMP9) in mesenchymal stem cells (MSCs). We found that BMP9 increased mRNA levels of TGF-β1 and COX-2 in C3H10T1/2 cells. BMP9-induced osteogenic markers were enhanced by TGF-β1 and reduced by TGF-βRI-specific inhibitor LY364947. BMP9 increased level of p-Smad2/3, which were either enhanced or reduced by COX-2 and its inhibitor NS398. BMP9-induced osteogenic markers were decreased by NS398 and it was partially reversed by TGF-β1. COX-2 increased BMP9-induce...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yan Deng Ling Li Jia-Hui Zhu Pei-Pei Li Yi-Xuan Deng Hong-Hong Luo Yuan-Yuan Yang Bai-Cheng He Yuxi Su Source Type: research

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW
This study aimed to investigate the role of lncRNA-IPW in the progression of choroidal neovascularization (CNV) and the underlying molecular mechanism. IPW was significantly up-regulated in the choroidal tissues of laser-induced CNV mice and in the endothelial cells in response to hypoxic stress. IPW silencing led to reduced formation of CNV in laser-induced CNV model and ex vivo choroidal sprouting model, which could achieve similar therapeutic effects of anti-VEGF on CNV formation. Silencing or transgenic overexpression of IPW could alter endothelial cell viability, proliferation, migration, and tube formation ability in...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Tian-Jing Yang Mu-Di Yao Ya-Nan Sun Xiu-Miao Li Qin Jiang Biao Yan Source Type: research

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202823. Online ahead of print.ABSTRACTEpithelial-mesenchymal transition (EMT) is an evolutionarily conserved developmental program that has been implicated in tumorigenesis and confers metastatic properties upon cancer cells. ZEB1 is a master transcription factor that activates the EMT process in various cancers. ZEB1 is reportedly degraded through the ubiquitin proteasome pathway, but the underlying molecular mechanism of this process remains largely unknown in hepatocellular carcinoma (HCC). Here, we identified ZEB1 as a substrate of the CRL4-DCAF15 (DDB1 and CUL4 ass...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Xiao Dong Yang Han Encheng Zhang Yuqi Wang Pingzhao Zhang Chenji Wang Lin Zhong Qi Li Source Type: research

lncRNA C2dat2 facilitates autophagy and apoptosis via the miR-30d-5p/DDIT4/mTOR axis in cerebral ischemia-reperfusion injury
This study first revealed that C2dat2/miR-30d-5p/DDIT4/mTOR forms a novel signaling pathway to facilitate autophagy and apoptosis induced by CIRI, contributing to the better understanding of the mechanisms of CIRI and enriching the ceRNA hypothesis in CIRI.PMID:33833132 | DOI:10.18632/aging.202824 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Qian Xu Ma Guohui Dandan Li Fanghui Bai Jintao Fang Gui Zhang Yuxin Xing Jiawei Zhou Yugang Guo Yunchao Kan Source Type: research

Genetic effect of < em > MTHFR < /em > C677T, A1298C, and A1793G polymorphisms on the age at onset, plasma homocysteine, and white matter lesions in Alzheimer's disease in the Chinese population
CONCLUSIONS: The genotype distributions of C677T and A1298C polymorphisms are associated with AD in the Chinese population. Moreover, AD patients with C677T polymorphism are prone to present an earlier onset, higher homocysteine level, and more severe WML.PMID:33833133 | DOI:10.18632/aging.202827 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yaling Jiang Xuewen Xiao Yafei Wen Meidan Wan Lu Zhou Xixi Liu Xin Wang Lina Guo Hui Liu Yafang Zhou Junling Wang Xinxin Liao Lu Shen Bin Jiao Source Type: research

Identification of novel functional CpG-SNPs associated with Type 2 diabetes and birth weight
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202828. Online ahead of print.ABSTRACTGenome-wide association studies (GWASs) have identified hundreds of genetic loci for type 2 diabetes (T2D) and birth weight (BW); however, a large proportion of the total trait heritability remains unexplained. The previous studies were generally focused on individual traits and largely failed to identify the majority of the variants that play key functional roles in the etiology of the disease. Here, we aim to identify novel functional loci for T2D, BW and the pleiotropic variants shared between them by performing a targeted condit...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Rui-Ke Liu Xu Lin Zun Wang Jonathan Greenbaum Chuan Qiu Chun-Ping Zeng Yong-Yao Zhu Jie Shen Hong-Wen Deng Source Type: research

COX-2 promotes the osteogenic potential of BMP9 through TGF- β1/p38 signaling in mesenchymal stem cells
This study investigated the effects of transforming growth factor-β1 (TGF-β1) and cyclooxygenase-2 (COX-2) on bone morphogenetic protein 9 (BMP9) in mesenchymal stem cells (MSCs). We found that BMP9 increased mRNA levels of TGF-β1 and COX-2 in C3H10T1/2 cells. BMP9-induced osteogenic markers were enhanced by TGF-β1 and reduced by TGF-βRI-specific inhibitor LY364947. BMP9 increased level of p-Smad2/3, which were either enhanced or reduced by COX-2 and its inhibitor NS398. BMP9-induced osteogenic markers were decreased by NS398 and it was partially reversed by TGF-β1. COX-2 increased BMP9-induce...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yan Deng Ling Li Jia-Hui Zhu Pei-Pei Li Yi-Xuan Deng Hong-Hong Luo Yuan-Yuan Yang Bai-Cheng He Yuxi Su Source Type: research

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW
This study aimed to investigate the role of lncRNA-IPW in the progression of choroidal neovascularization (CNV) and the underlying molecular mechanism. IPW was significantly up-regulated in the choroidal tissues of laser-induced CNV mice and in the endothelial cells in response to hypoxic stress. IPW silencing led to reduced formation of CNV in laser-induced CNV model and ex vivo choroidal sprouting model, which could achieve similar therapeutic effects of anti-VEGF on CNV formation. Silencing or transgenic overexpression of IPW could alter endothelial cell viability, proliferation, migration, and tube formation ability in...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Tian-Jing Yang Mu-Di Yao Ya-Nan Sun Xiu-Miao Li Qin Jiang Biao Yan Source Type: research

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202823. Online ahead of print.ABSTRACTEpithelial-mesenchymal transition (EMT) is an evolutionarily conserved developmental program that has been implicated in tumorigenesis and confers metastatic properties upon cancer cells. ZEB1 is a master transcription factor that activates the EMT process in various cancers. ZEB1 is reportedly degraded through the ubiquitin proteasome pathway, but the underlying molecular mechanism of this process remains largely unknown in hepatocellular carcinoma (HCC). Here, we identified ZEB1 as a substrate of the CRL4-DCAF15 (DDB1 and CUL4 ass...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Xiao Dong Yang Han Encheng Zhang Yuqi Wang Pingzhao Zhang Chenji Wang Lin Zhong Qi Li Source Type: research

lncRNA C2dat2 facilitates autophagy and apoptosis via the miR-30d-5p/DDIT4/mTOR axis in cerebral ischemia-reperfusion injury
This study first revealed that C2dat2/miR-30d-5p/DDIT4/mTOR forms a novel signaling pathway to facilitate autophagy and apoptosis induced by CIRI, contributing to the better understanding of the mechanisms of CIRI and enriching the ceRNA hypothesis in CIRI.PMID:33833132 | DOI:10.18632/aging.202824 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Qian Xu Ma Guohui Dandan Li Fanghui Bai Jintao Fang Gui Zhang Yuxin Xing Jiawei Zhou Yugang Guo Yunchao Kan Source Type: research

Genetic effect of < em > MTHFR < /em > C677T, A1298C, and A1793G polymorphisms on the age at onset, plasma homocysteine, and white matter lesions in Alzheimer's disease in the Chinese population
CONCLUSIONS: The genotype distributions of C677T and A1298C polymorphisms are associated with AD in the Chinese population. Moreover, AD patients with C677T polymorphism are prone to present an earlier onset, higher homocysteine level, and more severe WML.PMID:33833133 | DOI:10.18632/aging.202827 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yaling Jiang Xuewen Xiao Yafei Wen Meidan Wan Lu Zhou Xixi Liu Xin Wang Lina Guo Hui Liu Yafang Zhou Junling Wang Xinxin Liao Lu Shen Bin Jiao Source Type: research

Identification of novel functional CpG-SNPs associated with Type 2 diabetes and birth weight
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202828. Online ahead of print.ABSTRACTGenome-wide association studies (GWASs) have identified hundreds of genetic loci for type 2 diabetes (T2D) and birth weight (BW); however, a large proportion of the total trait heritability remains unexplained. The previous studies were generally focused on individual traits and largely failed to identify the majority of the variants that play key functional roles in the etiology of the disease. Here, we aim to identify novel functional loci for T2D, BW and the pleiotropic variants shared between them by performing a targeted condit...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Rui-Ke Liu Xu Lin Zun Wang Jonathan Greenbaum Chuan Qiu Chun-Ping Zeng Yong-Yao Zhu Jie Shen Hong-Wen Deng Source Type: research

COX-2 promotes the osteogenic potential of BMP9 through TGF- β1/p38 signaling in mesenchymal stem cells
This study investigated the effects of transforming growth factor-β1 (TGF-β1) and cyclooxygenase-2 (COX-2) on bone morphogenetic protein 9 (BMP9) in mesenchymal stem cells (MSCs). We found that BMP9 increased mRNA levels of TGF-β1 and COX-2 in C3H10T1/2 cells. BMP9-induced osteogenic markers were enhanced by TGF-β1 and reduced by TGF-βRI-specific inhibitor LY364947. BMP9 increased level of p-Smad2/3, which were either enhanced or reduced by COX-2 and its inhibitor NS398. BMP9-induced osteogenic markers were decreased by NS398 and it was partially reversed by TGF-β1. COX-2 increased BMP9-induce...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yan Deng Ling Li Jia-Hui Zhu Pei-Pei Li Yi-Xuan Deng Hong-Hong Luo Yuan-Yuan Yang Bai-Cheng He Yuxi Su Source Type: research

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW
This study aimed to investigate the role of lncRNA-IPW in the progression of choroidal neovascularization (CNV) and the underlying molecular mechanism. IPW was significantly up-regulated in the choroidal tissues of laser-induced CNV mice and in the endothelial cells in response to hypoxic stress. IPW silencing led to reduced formation of CNV in laser-induced CNV model and ex vivo choroidal sprouting model, which could achieve similar therapeutic effects of anti-VEGF on CNV formation. Silencing or transgenic overexpression of IPW could alter endothelial cell viability, proliferation, migration, and tube formation ability in...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Tian-Jing Yang Mu-Di Yao Ya-Nan Sun Xiu-Miao Li Qin Jiang Biao Yan Source Type: research

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202823. Online ahead of print.ABSTRACTEpithelial-mesenchymal transition (EMT) is an evolutionarily conserved developmental program that has been implicated in tumorigenesis and confers metastatic properties upon cancer cells. ZEB1 is a master transcription factor that activates the EMT process in various cancers. ZEB1 is reportedly degraded through the ubiquitin proteasome pathway, but the underlying molecular mechanism of this process remains largely unknown in hepatocellular carcinoma (HCC). Here, we identified ZEB1 as a substrate of the CRL4-DCAF15 (DDB1 and CUL4 ass...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Xiao Dong Yang Han Encheng Zhang Yuqi Wang Pingzhao Zhang Chenji Wang Lin Zhong Qi Li Source Type: research

lncRNA C2dat2 facilitates autophagy and apoptosis via the miR-30d-5p/DDIT4/mTOR axis in cerebral ischemia-reperfusion injury
This study first revealed that C2dat2/miR-30d-5p/DDIT4/mTOR forms a novel signaling pathway to facilitate autophagy and apoptosis induced by CIRI, contributing to the better understanding of the mechanisms of CIRI and enriching the ceRNA hypothesis in CIRI.PMID:33833132 | DOI:10.18632/aging.202824 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Qian Xu Ma Guohui Dandan Li Fanghui Bai Jintao Fang Gui Zhang Yuxin Xing Jiawei Zhou Yugang Guo Yunchao Kan Source Type: research

Genetic effect of < em > MTHFR < /em > C677T, A1298C, and A1793G polymorphisms on the age at onset, plasma homocysteine, and white matter lesions in Alzheimer's disease in the Chinese population
CONCLUSIONS: The genotype distributions of C677T and A1298C polymorphisms are associated with AD in the Chinese population. Moreover, AD patients with C677T polymorphism are prone to present an earlier onset, higher homocysteine level, and more severe WML.PMID:33833133 | DOI:10.18632/aging.202827 (Source: Aging)
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Yaling Jiang Xuewen Xiao Yafei Wen Meidan Wan Lu Zhou Xixi Liu Xin Wang Lina Guo Hui Liu Yafang Zhou Junling Wang Xinxin Liao Lu Shen Bin Jiao Source Type: research

Identification of novel functional CpG-SNPs associated with Type 2 diabetes and birth weight
Aging (Albany NY). 2021 Apr 4;13. doi: 10.18632/aging.202828. Online ahead of print.ABSTRACTGenome-wide association studies (GWASs) have identified hundreds of genetic loci for type 2 diabetes (T2D) and birth weight (BW); however, a large proportion of the total trait heritability remains unexplained. The previous studies were generally focused on individual traits and largely failed to identify the majority of the variants that play key functional roles in the etiology of the disease. Here, we aim to identify novel functional loci for T2D, BW and the pleiotropic variants shared between them by performing a targeted condit...
Source: Aging - April 9, 2021 Category: Biomedical Science Authors: Rui-Ke Liu Xu Lin Zun Wang Jonathan Greenbaum Chuan Qiu Chun-Ping Zeng Yong-Yao Zhu Jie Shen Hong-Wen Deng Source Type: research

Neurophysiological improvements in speech-in-noise task after short-term choir training in older adults
Aging (Albany NY). 2021 Apr 6;13. doi: 10.18632/aging.202931. Online ahead of print.ABSTRACTPerceiving speech in noise (SIN) is important for health and well-being and decreases with age. Musicians show improved speech-in-noise abilities and reduced age-related auditory decline, yet it is unclear whether short term music engagement has similar effects. In this randomized control trial we used a pre-post design to investigate whether a 12-week music intervention in adults aged 50-65 without prior music training and with subjective hearing loss improves well-being, speech-in-noise abilities, and auditory encoding and volunta...
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Sarah Hennessy Alison Wood Rand Wilcox Assal Habibi Source Type: research

Mendelian randomization as an approach to assess causal effects of inflammatory bowel disease on atrial fibrillation
CONCLUSION: As opposed to current assumptions, no substantial evidence was found to support a causal role of IBD in the development of AF.PMID:33824227 | DOI:10.18632/aging.202906 (Source: Aging)
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: LaiTe Chen GuoSheng Fu ChenYang Jiang Source Type: research

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration
Aging (Albany NY). 2021 Apr 6;13. doi: 10.18632/aging.202850. Online ahead of print.ABSTRACTStem cell approaches have become an attractive therapeutic option for intervertebral disc degeneration (IVDD). Nucleus pulposus mesenchymal stem cells (NP-MSCs) participate in the regeneration and homeostasis of the intervertebral disc (IVD), but the molecular mechanisms governing these processes remain to be elucidated. Acid-sensing ion channels (ASICs) which act as key receptors for extracellular protons in central and peripheral neurons, have been implicated in IVDD where degeneration is associated with reduced microenvironmental...
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Jingyu Ding Renjie Zhang Huimin Li Qiang Ji Xiaomin Cheng Rick Francis Thorne Hubert Hondermarck Xiaoying Liu Cailiang Shen Source Type: research

Association of vitamin D metabolites with cognitive function and brain atrophy in elderly individuals - the Austrian stroke prevention study
CONCLUSIONS: Vitamin D deficient individuals appear to have a modest reduction of memory function without structural brain atrophy. Future studies should explore if vitamin D supplementation can improve cognitive function.PMID:33825696 | DOI:10.18632/aging.202930 (Source: Aging)
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Sieglinde Zelzer Edith Hofer Andreas Meinitzer Eva Fritz-Petrin Sebastian Simstich Walter Goessler Reinhold Schmidt Markus Herrmann Source Type: research

Neurophysiological improvements in speech-in-noise task after short-term choir training in older adults
Aging (Albany NY). 2021 Apr 6;13. doi: 10.18632/aging.202931. Online ahead of print.ABSTRACTPerceiving speech in noise (SIN) is important for health and well-being and decreases with age. Musicians show improved speech-in-noise abilities and reduced age-related auditory decline, yet it is unclear whether short term music engagement has similar effects. In this randomized control trial we used a pre-post design to investigate whether a 12-week music intervention in adults aged 50-65 without prior music training and with subjective hearing loss improves well-being, speech-in-noise abilities, and auditory encoding and volunta...
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Sarah Hennessy Alison Wood Rand Wilcox Assal Habibi Source Type: research

Mendelian randomization as an approach to assess causal effects of inflammatory bowel disease on atrial fibrillation
CONCLUSION: As opposed to current assumptions, no substantial evidence was found to support a causal role of IBD in the development of AF.PMID:33824227 | DOI:10.18632/aging.202906 (Source: Aging)
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: LaiTe Chen GuoSheng Fu ChenYang Jiang Source Type: research

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration
Aging (Albany NY). 2021 Apr 6;13. doi: 10.18632/aging.202850. Online ahead of print.ABSTRACTStem cell approaches have become an attractive therapeutic option for intervertebral disc degeneration (IVDD). Nucleus pulposus mesenchymal stem cells (NP-MSCs) participate in the regeneration and homeostasis of the intervertebral disc (IVD), but the molecular mechanisms governing these processes remain to be elucidated. Acid-sensing ion channels (ASICs) which act as key receptors for extracellular protons in central and peripheral neurons, have been implicated in IVDD where degeneration is associated with reduced microenvironmental...
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Jingyu Ding Renjie Zhang Huimin Li Qiang Ji Xiaomin Cheng Rick Francis Thorne Hubert Hondermarck Xiaoying Liu Cailiang Shen Source Type: research

Association of vitamin D metabolites with cognitive function and brain atrophy in elderly individuals - the Austrian stroke prevention study
CONCLUSIONS: Vitamin D deficient individuals appear to have a modest reduction of memory function without structural brain atrophy. Future studies should explore if vitamin D supplementation can improve cognitive function.PMID:33825696 | DOI:10.18632/aging.202930 (Source: Aging)
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Sieglinde Zelzer Edith Hofer Andreas Meinitzer Eva Fritz-Petrin Sebastian Simstich Walter Goessler Reinhold Schmidt Markus Herrmann Source Type: research

Neurophysiological improvements in speech-in-noise task after short-term choir training in older adults
Aging (Albany NY). 2021 Apr 6;13. doi: 10.18632/aging.202931. Online ahead of print.ABSTRACTPerceiving speech in noise (SIN) is important for health and well-being and decreases with age. Musicians show improved speech-in-noise abilities and reduced age-related auditory decline, yet it is unclear whether short term music engagement has similar effects. In this randomized control trial we used a pre-post design to investigate whether a 12-week music intervention in adults aged 50-65 without prior music training and with subjective hearing loss improves well-being, speech-in-noise abilities, and auditory encoding and volunta...
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: Sarah Hennessy Alison Wood Rand Wilcox Assal Habibi Source Type: research

Mendelian randomization as an approach to assess causal effects of inflammatory bowel disease on atrial fibrillation
CONCLUSION: As opposed to current assumptions, no substantial evidence was found to support a causal role of IBD in the development of AF.PMID:33824227 | DOI:10.18632/aging.202906 (Source: Aging)
Source: Aging - April 7, 2021 Category: Biomedical Science Authors: LaiTe Chen GuoSheng Fu ChenYang Jiang Source Type: research