Multiwalled carbon nanotubes co-delivering sorafenib and epidermal growth factor receptor siRNA enhanced tumor-suppressing effect on liver cancer.
CONCLUSIONS: MWNT/Sor/siRNA exhibited a superior antitumor effect in vitro and in vivo. METHODS: The MWNT/Sor and MWNT/Sor/siRNA were prepared, and then the morphologies of MWNT/Sor/siRNA were analyzed. In vitro Sor release assay, siRNA stability and cellular uptake of MWNT/Sor/siRNA were performed as well. Next, the effects of MWNT, free Sor, free siRNA, MWNT/Sor and MWNT/Sor/siRNA were evaluated by colony-forming assay, and cell apoptosis assay in HepG2 cells. Meanwhile, the level of EGFR and proteins associated with apoptosis was tested. Furthermore, the anti-tumor effects of MWNT/Sor/siRNA on LC xenograft mice wer...
Source: Aging - January 13, 2021 Category: Biomedical Science Authors: Wen Z, Feng Y, Hu Y, Lian L, Huang H, Guo L, Chen S, Yang Q, Zhang M, Wan L, Xu K, Degejirifu D, Yan X Tags: Aging (Albany NY) Source Type: research

Combined transplantation of neural stem cells and bone marrow mesenchymal stem cells promotes neuronal cell survival to alleviate brain damage after cardiac arrest via microRNA-133b incorporated in extracellular vesicles.
Abstract Neural stem cell (NSC) transplantation has prevailed as a promising protective strategy for cardiac arrest (CA)-induced brain damage. Surprisingly, the poor survival of neuronal cells in severe hypoxic condition restricts the utilization of this cell-based therapy. Extracellular vesicles (EVs) transfer microRNAs (miRNAs) between cells are validated as the mode for the release of several therapeutic molecules. The current study reports that the bone marrow mesenchymal stem cells (BMSCs) interact with NSCs via EVs thereby affecting the survival of neuronal cells. Hypoxic injury models of neuronal cells were...
Source: Aging - January 12, 2021 Category: Biomedical Science Authors: Li F, Zhang J, Chen A, Liao R, Duan Y, Xu Y, Tao L Tags: Aging (Albany NY) Source Type: research

The burden of liver cirrhosis and underlying etiologies: results from the global burden of disease study 2017.
CONCLUSIONS: Though the number of people suffering from HBV and HCV decreases, liver cirrhosis is still a major threat to health. Additionally, the number of people with cirrhosis caused by alcohol and NASH continues to grow. Thus, more targeted and specific strategies should be established based on etiology and prevalence trends of liver cirrhosis. METHODS: We collected data based on Global Burden of Disease (GBD) 2017 study. The age standardized prevalence rate (ASR) and estimated annual percentage changes (EAPC) were used to estimate the trends in prevalence by population, etiologies and regions. PMID: 3343653...
Source: Aging - January 12, 2021 Category: Biomedical Science Authors: Zhai M, Long J, Liu S, Liu C, Li L, Yang L, Li Y, Shu B Tags: Aging (Albany NY) Source Type: research

Correlates of change in accelerometer-assessed total sedentary time and prolonged sedentary bouts among older English adults: results from five-year follow-up in the EPIC-Norfolk cohort.
CONCLUSION: Individuals in specific sub-groups (older, male, higher BMI) and who differentially participate in certain behaviours (less gardening, less walking and more television viewing) but not others increase their sedentary time at a higher rate than others; utilising this information could inform successful intervention content and targeting. PMID: 33431710 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Yerrakalva D, Hajna S, Wijndaele K, Westgate K, Khaw KT, Wareham N, Griffin SJ, Brage S Tags: Aging (Albany NY) Source Type: research

Telomere length as a function of age at population level parallels human survival curves.
Abstract Telomeres are subject to age related shortening which can be accelerated by oxidative stress and inflammation. Many studies have reported an inverse correlation between telomere length and survival, but such inverse correlation has not been always confirmed in different populations. We analyzed the trend of Leukocyte Telomere Length (LTL) as a function of age in a cohort of 516 subjects aged 65-106 years from Southern Italy. The trend of LTL obtained was quite similar to demographic survival curves reported with data of western societies. We observed a decrease of LTL after 70 years of age and then an inc...
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Crocco P, De Rango F, Dato S, Rose G, Passarino G Tags: Aging (Albany NY) Source Type: research

Analysis of the aging-induced changes in the motor ability structure using large population fitness test results.
Abstract Owing to confounding factors influencing the effect of aging, systematic analyses of age-related changes in motor ability are mostly limited to the use of animals whose diets and genetics can be controlled or the use of datasets of athletes who share similar lifestyles. However, we lack systematic methods for analyzing the effect of aging on the motor ability structure of the general public. We propose that principal component analysis (PCA) on fitness test results of a large sample may provide information on the aging-induced change in the motor ability structure of the general public. We complied the fi...
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Pathak P, Panday SB, Jung BK, Ahn J Tags: Aging (Albany NY) Source Type: research

Prognostic potential of cardiac structural and functional parameters and N-terminal propeptide of type III procollagen in predicting cardiac fibrosis one year after myocardial infarction with preserved left ventricular ejection fraction.
Abstract The aim of the study were to evaluate the prognostic potential of serum level of N-terminal propeptide procollagen type III (PIIINP) and heart parameters for predicting heart cardiac fibrosis 1 year after ST-segment elevation myocardial infarction (STEMI) with preserved left ventricular ejection fraction (LVEF). 68 patients with STEMI and preserved LVEF with acute heart failure of the I-III degree according to the Killip classification were examined. Echocardiography was performed and PIIINP levels were measured on days 1 and 12, as well as 1 year after STEMI. A year after STEMI, was performed contrast ma...
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Osokina A, Karetnikova V, Polikutina O, Ivanova A, Gruzdeva O, Dyleva Y, Kokov A, Brel N, Pecherina T, Barbarash O Tags: Aging (Albany NY) Source Type: research

Shorter telomere lengths in patients with severe COVID-19 disease.
;nez P, Blasco MA Abstract The incidence of severe manifestations of COVID-19 increases with age with older patients showing the highest mortality, suggesting that molecular pathways underlying aging contribute to the severity of COVID-19. One mechanism of aging is the progressive shortening of telomeres, which are protective structures at chromosome ends. Critically short telomeres impair the regenerative capacity of tissues and trigger loss of tissue homeostasis and disease. The SARS-CoV-2 virus infects many different cell types, forcing cell turn-over and regeneration to maintain tissue homeostasis. We hypothes...
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Sanchez-Vazquez R, Guío-Carrión A, Zapatero-Gaviria A, Martínez P, Blasco MA Tags: Aging (Albany NY) Source Type: research

Prenatal diagnosis of PLP1 duplication by single nucleotide polymorphism array in a family with Pelizaeus-Merzbacher disease.
This study preliminarily suggests that SNP array is a sensitive and accurate technology for identifying PLP1 duplication and is feasible for reliable diagnosis, including for the prenatal diagnosis of PMD resulting from PLP1 duplication. PMID: 33429367 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 11, 2021 Category: Biomedical Science Authors: Xue H, Yu A, Chen X, Lin N, Lin M, Huang H, Xu L Tags: Aging (Albany NY) Source Type: research

Reduced SULT2B1b expression alleviates ox-LDL-induced inflammation by upregulating miR-148-3P via inhibiting the IKK β/NF-κB pathway in macrophages.
In this study, after treatment with either ox-LDL alone or combined with transfection of siRNAs targeting SULT2B1b, IL-6, TNF-α, NF-κB, IKKβ and IκB mRNA and protein expression were determined in Raw264.7 cells by real-time PCR and Western blot, respectively. The proliferative capacity was determined by EdU staining and Cell Counting Kit-8. Our data demonstrated that SULT2B1b knockdown could reduce phosphorylated NF-κB levels and downregulate IKKβ protein levels. Additionally, IκB levels were increased and the proliferation of ox-LDL stimulated cells was inhibited after SULT2B1b sile...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Yin M, Lu J, Guo Z, Zhang Y, Liu J, Wu T, Guo K, Luo T, Guo Z Tags: Aging (Albany NY) Source Type: research

Identification of candidate genes encoding tumor-specific neoantigens in early- and late-stage colon adenocarcinoma.
Abstract Colon adenocarcinoma (COAD) is one of the most common gastrointestinal malignant tumors and is characterized by a high mortality rate. Here, we integrated whole-exome and RNA sequencing data from The Cancer Genome Atlas and investigated the mutational spectra of COAD-overexpressed genes to define clinically relevant diagnostic/prognostic signatures and to unmask functional relationships with both tumor-infiltrating immune cells and regulatory miRNAs. We identified 24 recurrently mutated genes (frequency> 5%) encoding putative COAD-specific neoantigens. Five of them (NEB, DNAH2, ABCA12, CENPF and CELSR1...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Wang C, Xue W, Zhang H, Fu Y Tags: Aging (Albany NY) Source Type: research

ALKBH5-mediated m6A demethylation of FOXM1 mRNA promotes progression of uveal melanoma.
In this study, we found that ALKBH5, a key component of the N6-methyladenosine (m6A) methyltransferase complex, was significantly elevated in uveal melanoma (UM) cell lines and that ALKBH5 downregulation inhibited tumor growth in vivo. High ALKBH5 expression predicted worse outcome in patients with UM. EP300-induced H3K27 acetylation activation increased ALKBH5 expression. Downregulation of ALKBH5 inhibited UM cell proliferation, migration, and invasion and increased apoptosis in vitro. Besides, ALKBH5 may promote UM metastasis by inducing epithelial-to-mesenchymal transition (EMT) via demethylation of FOXM1 mRNA, which in...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Hao L, Yin J, Yang H, Li C, Zhu L, Liu L, Zhong J Tags: Aging (Albany NY) Source Type: research

Tsukushi is a novel prognostic biomarker and correlates with tumor-infiltrating B cells in non-small cell lung cancer.
Abstract A recent study has reported that tsukushi (TSKU) may be related to the development of lung cancer. However, few studies focused on if TSKU associated with the prognosis and immune infiltration cells in non-small cell lung cancer (NSCLC). The effect of TSKU expression on prognosis with NSCLC was analyzed in the PrognoScan database and validated in The Cancer Genome Atlas. The composition of tumor infiltrating cells was quantified by methylation and expression data. We combined levels of tumor infiltrating cells with TSKU to evaluate the survival of patients. The analysis of a cohort (GSE31210, N=204) of lu...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Huang H, Zhang D, Fu J, Zhao L, Li D, Sun H, Liu X, Xu J, Tian T, Zhang L, Liu Y, Zhang Y, Zhao Y Tags: Aging (Albany NY) Source Type: research

Long noncoding RNA TPT1-AS1 promotes the progression and metastasis of colorectal cancer by upregulating the TPT1-mediated FAK and JAK-STAT3 signalling pathways.
This study aimed to determine the function and mechanism of action of TPT1-AS1 in the progression and metastasis of CRC. Elevated TPT1-AS1 levels were observed in CRC tissues. Furthermore, the high expression levels were found to be correlated with unfavourable clinicopathological characteristics in CRC. Cell function experiments demonstrated that TPT1-AS1 depletion impeded cell proliferation, migration and invasion and enhanced cell adhesion; it also attenuated tumorigenesis and metastasis in vivo. Additionally, TPT1-AS1 was predominately located in the nuclei of the cells and could upregulate the expression of TPT1 by re...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Zhang L, Ye F, Zuo Z, Cao D, Peng Y, Li Z, Huang J, Duan L Tags: Aging (Albany NY) Source Type: research

Gene signatures of 6-methyladenine regulators in women with lung adenocarcinoma and development of a risk scoring system: a retrospective study using The Cancer Genome Atlas database.
Abstract Although the emergence of new treatments has improved the prognosis of women with lung adenocarcinoma (LUAD), the emergence of drug resistance limits their clinical efficacy. Therefore, there is an urgent need to identify new targets and develop a risk scoring system to evaluate the prognosis of patients. 6-methyladenine (M6A), as the most common methyl modification in RNA modification, its clinicopathological features, diagnosis and prognostic value in lung cancer, especially in LUAD remain to be discussed. We analyzed the clinical and sequencing data of the female LUAD cohort from The Cancer Genome Atla...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Gao C, Zhuang J, Li H, Liu C, Zhou C, Liu L, Feng F, Sun C Tags: Aging (Albany NY) Source Type: research

Four hub genes regulate tumor infiltration by immune cells, antitumor immunity in the tumor microenvironment, and survival outcomes in lung squamous cell carcinoma patients.
In this study, we performed bioinformatics analyses to identify hub genes that regulate tumor infiltration by immune cells and antitumor immunity in the lung squamous cell carcinoma (LUSC). We identified 1738 robust and stable differentially expressed genes (DEGs) in the LUSC tissues based on robust rank aggregation (RRA) analysis of RNA-sequencing data from 5 GEO-LUSC datasets. We then classified TCGA-LUSC patients based on ssGSEA and ESTIMATE analyses of LUSC tissues into high, medium and low immunity subgroups showing significant differences in tumor purity. Weighted gene co-expression network analysis of the robust DEG...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Zhang T, Yang H, Sun B, Yao F Tags: Aging (Albany NY) Source Type: research

Deciphering the correlations between aging and constipation by metabolomics and network pharmacology.
Abstract From the points of view of phenomena and experience, aging and constipation are inextricably correlated. However, experimental support and underlying mechanisms are still lacking. The purpose of this study is to explore the relationships between aging and constipation from the perspectives of fecal metabolites and network pharmacology. The behavioral analyses of aging and constipation were carried out on both aging rats and constipation rats. We found that aging rats exhibited not only significant aging behaviors but also significant constipation behaviors, while constipation rats exhibited both significa...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Liu X, Zhao D, Zhao S, Li Z, Wang Y, Qin X Tags: Aging (Albany NY) Source Type: research

Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ER β2/5 pathway.
Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway. Aging (Albany NY). 2021 Jan 10;12: Authors: Hexiao T, Yuquan B, Lecai X, Yanhong W, Li S, Weidong H, Ming X, Xuefeng Z, Gaofeng P, Li Z, Minglin Z, Zheng T, Zetian Y, Xiao Z, Yi C, Lanuti M, Jinping Z Abstract Many studies have reported that estrogen (E2) promotes lung cancer by binding to nuclear estrogen receptors (ER), and altering ER related nuclear protein expressions. With the GEO database analysis, Human centromere protein F (CENPF) is highly expressed in lung adenocarcinoma (LUAD), and the co-ex...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Hexiao T, Yuquan B, Lecai X, Yanhong W, Li S, Weidong H, Ming X, Xuefeng Z, Gaofeng P, Li Z, Minglin Z, Zheng T, Zetian Y, Xiao Z, Yi C, Lanuti M, Jinping Z Tags: Aging (Albany NY) Source Type: research

MicroRNA-205-5p targets the HOXD9-Snail1 axis to inhibit triple negative breast cancer cell proliferation and chemoresistance.
Abstract MicroRNA-205 (miR-205) is believed to be related to the progress of tumors. HOXD9 has been proved to be expressed abnormally in several kinds of cancers. However, the role of miR-205 and HOXD9 in breast cancer remains unclear. The biological role of miR-205 in breast cancer cell proliferation and chemoresistance was investigated. The expression of miR-205 in clinical tissues and breast cancer cell lines were analyzed using quantitative real-time PCR test (qRT-PCR). Overexpression and knockdown models of miR-205 were established to study cell proliferation and chemotherapy-resistant. Moreover, the potentia...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Lin LF, Li YT, Han H, Lin SG Tags: Aging (Albany NY) Source Type: research

Estradiol is significantly associated with prognosis in non-surgical liver cancer patients: from bench to bedside.
Abstract There are rarely systematic studies to analyze the prognostic factors among non-surgical liver cancer patients. Whether there is a gender difference in the survival of non-surgical liver cancer patients and what may cause this difference is still unclear. A total of 12,312 non-surgical liver cancer patients were enrolled in this study. Age, race, sex, grade, tumor TNM stage, marital status, tumor size, and histological type were independent risk factors in liver cancer and were confirmed in the validation cohort. Before menopause, females demonstrated a better mean survival probability than males (39.4&pl...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Wang R, Liu Y, Sun H, Wang T, Li C, Fan J, Wang Z Tags: Aging (Albany NY) Source Type: research

The landscape of alternative splicing reveals novel events associated with tumorigenesis and the immune microenvironment in gastric cancer.
Abstract Alternative splicing (AS), contributing to vast protein diversity from a rather limited number of genes in eukaryotic transcripts, has emerged as an important signature for tumor initiation and progression. However, a systematic understanding of its functional impact and relevance to gastric cancer (GC) tumorigenesis is lacking. Differentially expressed AS (DEAS) was verified among GC-associated AS events based on RNA-seq profiles from the TCGA database. Functional enrichment analysis, unsupervised clustering analysis and prognostic models were used to infer the potential roles of DEAS events and their mo...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Lou S, Zhang J, Zhai Z, Yin X, Wang Y, Fang T, Xue Y Tags: Aging (Albany NY) Source Type: research

Hypoxia-inducible factor 2-alpha-dependent induction of IL-6 protects the heart from ischemia/reperfusion injury.
Abstract Myocardial ischemia-reperfusion injury (MIRI) results in increased myocardial infarct size and leads to poor clinical outcomes. Hypoxia-inducible factor 2-alpha (HIF2α) exerts myocardial protective effects during MIRI through as yet unclear mechanisms. Here, we show that knockdown of HIF2α with cardiotropic recombinant adeno-associated virus serotype 9 (rAAV9) in mouse hearts significantly increased the infarct sizes during myocardial ischemia/reperfusion (MI/R). In addition, HIF2α transcriptionally regulated the expression of interleukin 6 (IL-6) in cardiomyocytes to elicit cardioprotec...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Wu JW, Hu H, Li D, Ma LK Tags: Aging (Albany NY) Source Type: research

Tumor-associated macrophages promote pancreatic ductal adenocarcinoma progression by inducing epithelial-to-mesenchymal transition.
In this study, we investigated the role of tumor-associated macrophages (TAMs) in the progression of pancreatic ductal adenocarcinoma (PDAC). PDAC patients with higher levels of CD68+ TAMs exhibited shorter overall survival. In Transwell assays, PDAC cells incubated with TAMs or conditioned media from TAM cells (TAM-CM) showed higher migration and invasion rates than controls. PET/CT scan analysis of orthotopic PDAC model mice revealed greater primary tumor growth and liver metastasis in the TAM-CM treatment group than the controls. H&E staining of liver tissues showed significantly higher numbers of metastatic nodules...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Xiong C, Zhu Y, Xue M, Jiang Y, Zhong Y, Jiang L, Shi M, Chen H Tags: Aging (Albany NY) Source Type: research

Exploration of the immune cell infiltration related gene signature in the prognosis of melanoma.
In this study, we established and verified a 7-gene prognostic signature. Melanoma patients from the Cancer Genome Atlas (TCGA) were separated into a low-risk group and a high-risk group using the median risk score. Receiver operating characteristic (ROC) analysis for overall survival (OS) showed that the area under the curve (AUC) was 0.701 for 1 year, 0.726 for 3 years, and 0.745 for 5 years, respectively. Moreover, a nomogram was constructed as a practical prognostic tool, and the AUC was 0.829 for 3 years, and 0.803 for 5 years, respectively. Furthermore, we validated the above results in two datasets from the Gene Exp...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Zeng Y, Zeng Y, Yin H, Chen F, Wang Q, Yu X, Zhou Y Tags: Aging (Albany NY) Source Type: research

Phenotyping of immune and endometrial epithelial cells in endometrial carcinomas revealed by single-cell RNA sequencing.
In this study, a cellular map of endometrial carcinoma was generated by profiling 30,780 cells isolated from tumor and paratumor tissues from five patients using single-cell RNA sequencing. 7 cell types in lymphocytes, 7 types in myeloid cells and 3 types in endometrial epithelial cells were identified. Distinct CD8+ T cell states and different monocyte-macrophage populations were discovered, among which exhausted CD8+ T cells and macrophages were preferentially enriched in tumor. Both CD8+ T cells and macrophages comport with continuous activation model. Gene expression patterns examination and gene ontology enrichment an...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Guo YE, Li Y, Cai B, He Q, Chen G, Wang M, Wang K, Wan X, Yan Q Tags: Aging (Albany NY) Source Type: research

A novel Foxp3-related immune prognostic signature for glioblastoma multiforme based on immunogenomic profiling.
This study analyzed the effect of Foxsp3 on the immune microenvironment and constructed a Foxp3-related immune prognostic signature (IPS)for predicting prognosis in glioblastoma multiforme (GBM). Immunohistochemistry (IHC) staining for Foxp3 was performed in 72 high-grade glioma specimens. RNA-seq data from 152 GBM samples were obtained from The Cancer Genome Atlas database (TCGA) and divided into two groups, Foxp3 High (Foxp3_H) and Foxp3 Low (Foxp3_L), based on Foxp3 expression. We systematically analyzed the influence of Foxp3 on the immune microenvironment. Least Absolute Shrinkage and Selection Operator (LASSO) Cox an...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Guo XY, Zhang GH, Wang ZN, Duan H, Xie T, Liang L, Cui R, Hu HR, Wu Y, Dong JJ, He ZQ, Mou YG Tags: Aging (Albany NY) Source Type: research

Late-onset epilepsy and subsequent increased risk of dementia.
Abstract Inflammation is considered as a key pathogenesis factor of dementia and epilepsy. However, epilepsy's association with dementia, particularly its role in the development of dementia, remains unclear. To evaluate the association between epilepsy and the risk of dementia, in Taiwan, we have now conducted a retrospective cohort study comprising 675 individuals (age, ≥50 years) with epilepsy and 2,025 matched control subjects without epilepsy. In order to match individuals diagnosed with epilepsy with those with no diagnosis of epilepsy (comparison cohort), we utilized exact matching at a ratio of 1:3. Com...
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Tsai ZR, Zhang HW, Tseng CH, Peng HC, Kok VC, Li GP, Hsiung CA, Hsu CY Tags: Aging (Albany NY) Source Type: research

Lung adenocarcinoma patients have higher risk of SARS-CoV-2 infection.
In conclusion, it was assumed that miR-125b-5p-ACE2-IL6 axis could alter the risk of SARS-CoV-2 infection in lung adenocarcinoma patients. PMID: 33429366 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 10, 2021 Category: Biomedical Science Authors: Chen L, Liu Y, Wu J, Deng C, Tan J, Liu H, Zhong L Tags: Aging (Albany NY) Source Type: research

Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity.
In this study, SH extract was tested on human keratinocyte cell line (HaCaT) exposed to stress factors related to premature aging of cells such as free radicals and ultraviolet B radiation. We confirmed that SH acts as scavenger of ROS and found its ability to restore the skin barrier integrity by reinforcing the cytoskeleton structure, sealing the tight junctions and increasing the migration rate of cells. Given these results, this work becomes relevant, identifying Salvia haenkei as a compound useful for anti-aging skin treatment in clinical performance. PMID: 33424011 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 8, 2021 Category: Biomedical Science Authors: Cocetta V, Cadau J, Saponaro M, Giacomini I, Dall'Acqua S, Sut S, Catanzaro D, Orso G, Miolo G, Menilli L, Pagetta A, Ragazzi E, Montopoli M Tags: Aging (Albany NY) Source Type: research

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.
In conclusion, this study reveals that the GAS5/miR-217/LHPP pathway reduces NSCLC cisplatin resistance and that LHPP may serve as a potential therapeutic target for NSCLC cisplatin resistance. PMID: 33418541 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 8, 2021 Category: Biomedical Science Authors: Yang X, Meng L, Zhong Y, Hu F, Wang L, Wang M Tags: Aging (Albany NY) Source Type: research

Function of Deptor and its roles in hematological malignancies.
Abstract Deptor is a protein that interacts with mTOR and that belongs to the mTORC1 and mTORC2 complexes. Deptor is capable of inhibiting the kinase activity of mTOR. It is well known that the mTOR pathway is involved in various signaling pathways that are involved with various biological processes such as cell growth, apoptosis, autophagy, and the ER stress response. Therefore, Deptor, being a natural inhibitor of mTOR, has become very important in its study. Because of this, it is important to research its role regarding the development and progression of human malignancies, especially in hematologic malignanci...
Source: Aging - January 7, 2021 Category: Biomedical Science Authors: Morales-Martinez M, Lichtenstein A, Vega MI Tags: Aging (Albany NY) Source Type: research

Prognostic and clinical significance of modified glasgow prognostic score in pancreatic cancer: a meta-analysis of 4,629 patients.
In this study, we evaluated the association of modified Glasgow Prognostic Score (mGPS) with prognosis in pancreatic cancer (PC) by performing a meta-analysis. Potentially eligible studies were shortlisted by searching PubMed, Embase, Web of Science, Scopus, and the Cochrane Library. A total of 4,629 patients with PC from 25 studies were finally included in this meta-analysis. Meta-analyses were performed using a random-effects model or fixed-effect model according to heterogeneity. We pooled the hazard ratios (HRs) with 95% confidence intervals (CIs) to estimate the association between mGPS and overall survival (OS). The ...
Source: Aging - January 6, 2021 Category: Biomedical Science Authors: Wu D, Wang X, Shi G, Sun H, Ge G Tags: Aging (Albany NY) Source Type: research

Exercise reduces metabolic burden while altering the immune system in aged mice.
In this study, we confirmed that 4-week treadmill exercise sufficiently improved metabolic function, including increased lean mass and decreased fat mass, in 88-week-old mice. The expression level of the senescence marker p16 in the white adipose tissue (WAT) was decreased after 4-weeks of exercise. Exercise induced changes in the profiles of immune-cell subsets, including natural killer (NK) cells, central memory CD8+ T cells, eosinophils, and neutrophils, in the stromal vascular fraction of WAT. In addition, it has been shown through transcriptome analysis of WAT that exercise can activate pathways involved in the intera...
Source: Aging - January 6, 2021 Category: Biomedical Science Authors: Yoon KJ, Ahn A, Park SH, Kwak SH, Kwak SE, Lee W, Yang YR, Kim M, Shin HM, Kim HR, Moon HY Tags: Aging (Albany NY) Source Type: research

NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock.
In conclusion, NLRC4 silencing ameliorates lung injury and inflammation induced by septic shock by negatively regulating the NLR pathway. PMID: 33406504 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 6, 2021 Category: Biomedical Science Authors: Wang SS, Yan CS, Luo JM Tags: Aging (Albany NY) Source Type: research

Serum response factor, a novel early diagnostic biomarker of acute kidney injury.
CONCLUSION: SRF is remarkably upregulated in early (before 24 h) AKI and can replace Scr as a potential new early diagnostic biomarker of AKI. PMID: 33406503 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - January 5, 2021 Category: Biomedical Science Authors: Zhao L, Li C, Guan C, Song N, Luan H, Luo C, Jiang W, Bu Q, Wang Y, Che L, Xu Y Tags: Aging (Albany NY) Source Type: research

Dexrazoxane ameliorates radiation-induced heart disease in a rat model.
Abstract Treatment of thoracic tumors with radiotherapy can lead to severe cardiac injury. We investigated the effects of dexrazoxane, a USFDA-approved cardioprotective drug administered with chemotherapy, on radiation-induced heart disease (RIHD) in a rat model. Male Sprague-Dawley rats were irradiated with a single dose of 20 Gy to the heart and treated with dexrazoxane at the time of irradiation and for 12 subsequent weeks. Dexrazoxane suppressed radiation-induced myocardial apoptosis and significantly reversed changes in serum cardiac troponin I levels and histopathological characteristics six months post-radi...
Source: Aging - January 2, 2021 Category: Biomedical Science Authors: Li L, Nie X, Zhang P, Huang Y, Ma L, Li F, Yi M, Qin W, Yuan X Tags: Aging (Albany NY) Source Type: research

ARDD 2020: from aging mechanisms to interventions.
ova V, de Grey A, He WW, Hoeijmakers J, Hoffmann E, Horvath S, Houtkooper RH, Jensen MK, Jensen MB, Kane A, Kassem M, de Keizer P, Kennedy B, Karsenty G, Lamming DW, Lee KF, MacAulay N, Mamoshina P, Mellon J, Molenaars M, Moskalev A, Mund A, Niedernhofer L, Osborne B, Pak HH, Parkhitko A, Raimundo N, Rando TA, Rasmussen LJ, Reis C, Riedel CG, Franco-Romero A, Schumacher B, Sinclair DA, Suh Y, Taub PR, Toiber D, Treebak JT, Valenzano DR, Verdin E, Vijg J, Young S, Zhang L, Bakula D, Zhavoronkov A, Scheibye-Knudsen M Abstract Aging is emerging as a druggable target with growing interest from academia, industry and i...
Source: Aging - December 30, 2020 Category: Biomedical Science Authors: Mkrtchyan GV, Abdelmohsen K, Andreux P, Bagdonaite I, Barzilai N, Brunak S, Cabreiro F, de Cabo R, Campisi J, Cuervo AM, Demaria M, Ewald CY, Fang EF, Faragher R, Ferrucci L, Freund A, Silva-García CG, Georgievskaya A, Gladyshev VN, Glass DJ, Gorbunova V Tags: Aging (Albany NY) Source Type: research

Age-related transcriptome changes in melanoma patients with tumor-positive sentinel lymph nodes.
This study uses three independent datasets of RNA samples from patients with melanoma metastatic to the SLN to identify age-related transcriptome changes in SLNs and their association with outcome. Microarray was applied to the first dataset of 97 melanoma patients. NanoString was performed in the second dataset to identify the specific immune genes and pathways that are associated with recurrence in younger versus older patients. qRT-PCR analysis was used in the third dataset of 36 samples to validate the differentially expressed genes (DEGs) from microarray and NanoString. These analyses show that FOS, NR4A, and ITGB1 ge...
Source: Aging - December 29, 2020 Category: Biomedical Science Authors: Menefee DS, McMasters A, Pan J, Li X, Xiao D, Waigel S, Zacharias W, Rai SN, McMasters KM, Hao H Tags: Aging (Albany NY) Source Type: research

Time gap between the onset and diagnosis in Werner syndrome: a nationwide survey and the 2020 registry in Japan.
to M, Yokote K Abstract Patients with Werner syndrome present with diverse signs of aging that begin in adolescence. A Japanese nationwide survey was conducted to establish a registry that could clarify the disease profile of patients with Werner syndrome. The questionnaires were sent to 7888 doctors. The survey identified 116 patients diagnosed with Werner syndrome based on the diagnosis criteria. Forty patients were enrolled in the registry. Data on clinical symptoms, treatment information, and laboratory examination from patients who provided informed consent were collected. The data at enrollment were analyzed...
Source: Aging - December 29, 2020 Category: Biomedical Science Authors: Koshizaka M, Maezawa Y, Maeda Y, Shoji M, Kato H, Kaneko H, Ishikawa T, Kinoshita D, Kobayashi K, Kawashima J, Sekiguchi A, Motegi SI, Nakagami H, Yamada Y, Tsukamoto S, Taniguchi A, Sugimoto K, Shoda Y, Hashimoto K, Yoshimura T, Suzuki D, Kuzuya M, Takem Tags: Aging (Albany NY) Source Type: research

Melatonin protects against oxybenzone-induced deterioration of mouse oocytes during maturation.
In this study, we investigated the detrimental effects of OBZ on oocyte maturation and the protective roles of melatonin (MT) in OBZ-exposed mouse models. Our in vitro and in vivo results showed that OBZ suppressed oocyte maturation, while MT attenuated the meiotic defects induced by OBZ. In addition, OBZ facilitated H3K4 demethylation by increasing the expression of the Kdm5 family of genes, elevating ROS levels, decreasing GSH, impairing mitochondrial quality, and disrupting spindle configuration in oocytes. However, MT treatment resulted in significant protection against OBZ-induced damage during oocyte maturation and i...
Source: Aging - December 29, 2020 Category: Biomedical Science Authors: Jin L, Zhu HY, Kang XJ, Lin LP, Zhang PY, Tan T, Yu Y, Fan Y Tags: Aging (Albany NY) Source Type: research

Neuroprotective effects and mechanisms of action of nicotinamide mononucleotide (NMN) in a photoreceptor degenerative model of retinal detachment.
In conclusion, NMN administration exerts neuroprotective effects on photoreceptors after RD and oxidative injury, suggesting a therapeutic avenue to treating photoreceptor degeneration. PMID: 33373320 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - December 29, 2020 Category: Biomedical Science Authors: Chen X, Amorim JA, Moustafa GA, Lee JJ, Yu Z, Ishihara K, Iesato Y, Barbisan P, Ueta T, Togka KA, Lu L, Sinclair DA, Vavvas DG Tags: Aging (Albany NY) Source Type: research

Subanesthetic isoflurane abates ROS-activated MAPK/NF- κB signaling to repress ischemia-induced microglia inflammation and brain injury.
Subanesthetic isoflurane abates ROS-activated MAPK/NF-κB signaling to repress ischemia-induced microglia inflammation and brain injury. Aging (Albany NY). 2020 Dec 28;12: Authors: Yao Z, Liu N, Zhu X, Wang L, Zhao Y, Liu Q, Gao C, Li J Abstract Isoflurane (ISO) elicits protective effects on ischemia-induced brain injury. We investigated whether sub-anesthetic (0.7%) ISO post-conditioning attenuates the inflammation and apoptosis in oxygen-glucose deprivation (OGD)-insulted co-cultures (microglia and neurons) in vitro and the brain injury of the middle cerebral arterial occlusion (MCAO) rat. We d...
Source: Aging - December 28, 2020 Category: Biomedical Science Authors: Yao Z, Liu N, Zhu X, Wang L, Zhao Y, Liu Q, Gao C, Li J Tags: Aging (Albany NY) Source Type: research

MiR-223/NFAT5 signaling suppresses arterial smooth muscle cell proliferation and motility in vitro.
Abstract Aberrant proliferation and migration of vascular smooth muscle cells contributes to cardiovascular diseases (CVDs), including atherosclerosis. MicroRNA-223 (miR-223) protects against atherosclerotic CVDs. We investigated the contribution of miR-223 to platelet-derived growth factor-BB (PDGF-BB)-induced proliferation and migration of human aortic smooth muscle cells (HASMCs). We found that miR-223 was downregulated in PDGF-BB-treated HASMCs in a dose- and time-dependent manner, while nuclear factor of activated T cells 5 (NFAT5) was upregulated. Gain- and loss-of-function studies demonstrated that miR-223 ...
Source: Aging - December 28, 2020 Category: Biomedical Science Authors: Su F, Shi M, Zhang J, Zheng Q, Wang H, Li X, Chen J Tags: Aging (Albany NY) Source Type: research

The effects of early-life growth hormone intervention on tissue specific histone H3 modifications in long-lived Ames dwarf mice.
This study serves as an initial, but important step in elucidating the epigenetic mechanisms by which hormonal signals during early life can influence aging and longevity in mammals. PMID: 33378746 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - December 28, 2020 Category: Biomedical Science Authors: Zhang F, Icyuz M, Bartke A, Sun LY Tags: Aging (Albany NY) Source Type: research

Age-associated deficient recruitment of 53BP1 in G1 cells directs DNA double-strand break repair to BRCA1/CtIP-mediated DNA-end resection.
We describe here a deficient recruitment of 53BP1 to DSB sites in G1 cells, probably influenced by an altered epigenetic regulation. 53BP1 absence at some DSBs is responsible for the age-associated DNA repair defect, as it permits the ectopic formation of BRCA1 foci while still in the G1 phase. CtIP and RPA foci are also formed in G1 cells from aged donors, but RAD51 is not recruited, thus indicating that extensive DNA-end resection occurs in these breaks although HR is not triggered. These results suggest an age-associated switch of DSB repair from canonical to highly mutagenic alternative mechanisms that promote the form...
Source: Aging - December 27, 2020 Category: Biomedical Science Authors: Anglada T, Genescà A, Martín M Tags: Aging (Albany NY) Source Type: research

Immunometabolism at the cornerstone of inflammaging, immunosenescence, and autoimmunity in COVID-19.
Abstract Inflammaging constitutes the common factor for comorbidities predisposing to severe COVID-19. Inflammaging leads to T-cell senescence, and immunosenescence is linked to autoimmune manifestations in COVID-19. As in SLE, metabolic dysregulation occurs in T-cells. Targeting this T-cell dysfunction opens the field for new therapeutic strategies to prevent severe COVID-19. Immunometabolism-mediated approaches such as rapamycin, metformin and dimethyl fumarate, may optimize COVID-19 treatment of the elderly and patients at risk for severe disease. PMID: 33361522 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - December 27, 2020 Category: Biomedical Science Authors: Omarjee L, Perrot F, Meilhac O, Mahe G, Bousquet G, Janin A Tags: Aging (Albany NY) Source Type: research

Obesity induced by high-fat diet is associated with critical changes in biological and molecular functions of mesenchymal stromal cells present in visceral adipose tissue.
Galderisi U Abstract The mesenchymal stromal cells (MSCs) residing within the stromal component of visceral adipose tissue appear to be greatly affected by obesity, with impairment of their functions and presence of senescence. To gain further insight into these phenomena, we analyzed the changes in total proteome content and secretome of mouse MSCs after a high-fat diet (HFD) treatment compared to a normal diet (ND). In healthy conditions, MSCs are endowed with functions mainly devoted to vesicle trafficking. These cells have an immunoregulatory role, affecting leukocyte activation and migration, acute inflammati...
Source: Aging - December 27, 2020 Category: Biomedical Science Authors: Acar MB, Ayaz-Güner Ş, Di Bernardo G, Güner H, Murat A, Peluso G, Özcan S, Galderisi U Tags: Aging (Albany NY) Source Type: research

Arterial elementary calcium signaling in aging.
PMID: 33361526 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - December 27, 2020 Category: Biomedical Science Authors: Kassmann M, Fan G, Gollasch M Tags: Aging (Albany NY) Source Type: research

A DNA methylation age predictor for zebrafish.
This study highlights the potential further use of DNA methylation as an age estimation method in non-mammalian vertebrate species. PMID: 33353889 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - December 23, 2020 Category: Biomedical Science Authors: Mayne B, Korbie D, Kenchington L, Ezzy B, Berry O, Jarman S Tags: Aging (Albany NY) Source Type: research

Aberrant RhoA activation in macrophages increases senescence-associated secretory phenotypes and ectopic calcification in muscular dystrophic mice.
Abstract Duchenne Muscular Dystrophy (DMD) patients often suffer from both muscle wasting and osteoporosis. Our previous studies have revealed reduced regeneration potential in skeletal muscle and bone, concomitant with ectopic calcification of soft tissues in double knockout (dKO, dystrophin-/-; utrophin-/-) mice, a severe murine model for DMD. We found significant involvement of RhoA/ROCK (Rho-Associated Protein Kinase) signaling in mediating ectopic calcification of muscles in dKO mice. However, the cellular identity of these RhoA+ cells, and the role that RhoA plays in the chronic inflammation-associated patho...
Source: Aging - December 23, 2020 Category: Biomedical Science Authors: Mu X, Lin CY, Hambright WS, Tang Y, Ravuri S, Lu A, Matre P, Chen W, Gao X, Cui Y, Zhong L, Wang B, Huard J Tags: Aging (Albany NY) Source Type: research