Replicative and radiation-induced aging: a comparison of gene expression profiles.
Abstract All living organisms are subject to the aging process and experience the effect of ionizing radiation throughout their life. There have been a number of studies that linked ionizing radiation process to accelerated aging, but comprehensive signalome analysis of both processes was rarely conducted. Here we present a comparative signaling pathway based analysis of the transcriptomes of fibroblasts irradiated with different doses of ionizing radiation, replicatively aged fibroblasts and fibroblasts collected from young, middle age and old patients. We demonstrate a significant concordance between irradiation...
Source: Aging - April 19, 2019 Category: Biomedical Science Authors: Aliper AM, Bozdaganyan ME, Orekhov PS, Zhavoronkov A, Osipov AN Tags: Aging (Albany NY) Source Type: research

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs).
ti MP Abstract Here, we devised a new strategy for eradicating cancer stem cells (CSCs), via a "synthetic-metabolic" approach, involving two FDA-approved antibiotics and a dietary vitamin supplement. This approach was designed to induce a "rho-zero-like" phenotype in cancer cells. This strategy effectively results in the synergistic eradication of CSCs, using vanishingly small quantities of two antibiotics. The 2 metabolic targets are i) the large mitochondrial ribosome and ii) the small mitochondrial ribosome. Azithromycin inhibits the large mitochondrial ribosome as an off-target side-effect....
Source: Aging - April 19, 2019 Category: Biomedical Science Authors: Fiorillo M, Tóth F, Sotgia F, Lisanti MP Tags: Aging (Albany NY) Source Type: research

The widespread increase in inter-individual variability of gene expression in the human brain with age.
In this study, we test whether transcriptional noise shows an increase with age, which would be expected from stochastic theories. Using human brain transcriptome dataset, we analyzed the heterogeneity in the transcriptome for individual genes and functional pathways, employing different analysis methods and pre-processing steps. We show that unlike expression level changes, changes in heterogeneity are highly dependent on the methodology and the underlying assumptions. Although the particular set of genes that can be characterized as differentially variable is highly dependent on the methods, we observe a consistent incre...
Source: Aging - April 19, 2019 Category: Biomedical Science Authors: Kedlian VR, Donertas HM, Thornton JM Tags: Aging (Albany NY) Source Type: research

A role of the 53BP1 protein in genome protection: structural and functional characteristics of 53BP1-dependent DNA repair.
M, Suchánková J Abstract Nuclear architecture plays a significant role in DNA repair mechanisms. It is evident that proteins involved in DNA repair are compartmentalized in not only spontaneously occurring DNA lesions or ionizing radiation-induced foci (IRIF), but a specific clustering of these proteins can also be observed within the whole cell nucleus. For example, 53BP1-positive and BRCA1-positive DNA repair foci decorate chromocenters and can appear close to nuclear speckles. Both 53BP1 and BRCA1 are well-described factors that play an essential role in double-strand break (DSB) repair. These pro...
Source: Aging - April 17, 2019 Category: Biomedical Science Authors: Bártová E, Legartová S, Dundr M, Suchánková J Tags: Aging (Albany NY) Source Type: research

Cognitive fatigue and cortical-striatal network in old age.
Abstract Cognitive fatigue (CF) is among the most common and disturbing aging symptoms, and substantially interferes with activities demanding sustained mental effort. Here we examined the relationship between the cortical-striatal network and CF (assessed by the 18-item visual analogue scale) when a group of cognitively and physically healthy older adults participated in a 30-minute cognitively fatiguing task-related fMRI experiment. We also explored whether CF would interfere with the "Posterior-Anterior Shifting in Aging" (PASA) phenomenon, an aging-associated neural reliance on frontal regions to sup...
Source: Aging - April 17, 2019 Category: Biomedical Science Authors: Ren P, Anderson AJ, McDermott K, Baran TM, Lin F Tags: Aging (Albany NY) Source Type: research

Resveratrol prevents sarcopenic obesity by reversing mitochondrial dysfunction and oxidative stress via the PKA/LKB1/AMPK pathway.
CONCLUSIONS: Resveratrol prevented high-fat diet-induced muscle atrophy in aged rats by reversing mitochondrial dysfunction and oxidative stress, which was partially mediated by the PKA/LKB1/AMPK pathway. These findings indicate that resveratrol might have potential uses for the prevention and treatment of sarcopenic obesity. PMID: 30988232 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 15, 2019 Category: Biomedical Science Authors: Huang Y, Zhu X, Chen K, Lang H, Zhang Y, Hou P, Ran L, Zhou M, Zheng J, Yi L, Mi M, Zhang Q Tags: Aging (Albany NY) Source Type: research

Role of global aberrant alternative splicing events in papillary thyroid cancer prognosis.
CONCLUSIONS: Our analysis provides the status of splicing events involved in the progression and may represent an underappreciated hallmark of PTC. PMID: 30986203 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 15, 2019 Category: Biomedical Science Authors: Lin P, He RQ, Huang ZG, Zhang R, Wu HY, Shi L, Li XJ, Li Q, Chen G, Yang H, He Y Tags: Aging (Albany NY) Source Type: research

Pyridoxine 5'-phosphate oxidase is correlated with human breast invasive ductal carcinoma development.
This study aims to evaluate the biological function and the regulatory mechanism of PNPO in human breast invasive ductal carcinoma (IDC). We unveiled for the first time that PNPO was upregulated in patients with IDC and was correlated with the overall survival of patients with metastasis at the later stages. Suppression of PNPO inhibited breast cancer cell proliferation, migration, invasion and colony formation, arrested cell cycle at the G2/M phase and induced cell apoptosis. PNPO was positively correlated with lncRNA MALAT1 which was negatively correlated with miR-216b-5p. PNPO was down-regulated and up-regulated by miR-...
Source: Aging - April 14, 2019 Category: Biomedical Science Authors: Ren W, Guan W, Zhang J, Wang F, Xu G Tags: Aging (Albany NY) Source Type: research

Early-life vancomycin treatment promotes airway inflammation and impairs microbiome homeostasis.
Abstract Several studies have reported that gut and lung microbiomes are involved in the process of asthma pathogenesis. However, it remains unclear how perinatal or early-life antibiotic intervention affect adult allergic airway inflammation. We assigned C57BL/6 mice randomly to four experimental groups: normal saline control (NS), ovalbumin (OVA), vancomycin pretreated NS (VAN-NS), and vancomycin pretreated OVA (VAN-OVA). The vancomycin groups were orally given the drug from gestational day 14 to 6 week. An OVA-induced asthma model was then established at 6 weeks of age, and airway inflammation was evaluated. In...
Source: Aging - April 13, 2019 Category: Biomedical Science Authors: Yang X, Feng H, Zhan X, Zhang C, Cui R, Zhong L, Ying S, Chen Z Tags: Aging (Albany NY) Source Type: research

In mice transgenic for IGF1 under keratin-14 promoter, lifespan is decreased and the rates of aging and thymus involution are accelerated.
Abstract IGF1 signaling is supposedly a key lifespan determinant in metazoans. However, controversial lifespan data were obtained with different means used to modify IGF1 or its receptor (IGF1R) expression in mice. The emerging puzzle lacks pieces of evidence needed to construct a coherent picture. We add to the available evidence by using the Gompertz model (GM), with account for the artifactual component of the Strehler-Mildvan correlation between its parameters, to compare the survival patterns of female FVB/N and FVB/N-derived K14/mIGF1 mice. In K14/mIGF1 vs. FVB/N mice, the rate of aging (γ) is markedly...
Source: Aging - April 13, 2019 Category: Biomedical Science Authors: Anisimov VN, Labunets IF, Popovich IG, Tyndyk ML, Yurova MN, Golubev AG Tags: Aging (Albany NY) Source Type: research

MiR-204 reduces cisplatin resistance in non-small cell lung cancer through suppression of the caveolin-1/AKT/Bad pathway.
Abstract Non-small cell lung cancer (NSCLC) is the most common and lethal human malignant tumor worldwide. Platinum-based chemotherapy is still the mainstay of treatment for NSCLC. However, long-term chemotherapy usually induces serious drug resistance in NSCLC cells. Accordingly, treatment strategies that reverse the resistance of NSCLC cells against platinum-based drugs may have considerable clinical value. In the present study, we observed significant upregulation of CAV-1 expression and a significant decrease of miR-204 expression in cisplatin-resistant A549 (CR-A549) and cisplatin-resistant PC9 (CR-PC9) cells...
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Huang G, Lou T, Pan J, Ye Z, Yin Z, Li L, Cheng W, Cao Z Tags: Aging (Albany NY) Source Type: research

Alternative splicing, FOXP3 and cardiovascular disease.
PMID: 30981208 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Joly AL, Andersson J Tags: Aging (Albany NY) Source Type: research

Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease.
Abstract In celiac disease (CD), an intolerance to dietary gluten/gliadin, antigenic gliadin peptides trigger an HLA-DQ2/DQ8-restricted adaptive Th1 immune response. Epithelial stress, induced by other non-antigenic gliadin peptides, is required for gliadin to become fully immunogenic. We found that cystic-fibrosis-transmembrane-conductance-regulator (CFTR) acts as membrane receptor for gliadin-derived peptide P31-43, as it binds to CFTR and impairs its channel function. P31-43-induced CFTR malfunction generates epithelial stress and intestinal inflammation. Maintaining CFTR in an active open conformation by the C...
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Esposito S, Villella VR, Ferrari E, Monzani R, Tosco A, Rossin F, D'Eletto M, Castaldo A, Luciani A, Silano M, Bona G, Marseglia GL, Romani L, Piacentini M, Raia V, Kroemer G, Maiuri L Tags: Aging (Albany NY) Source Type: research

Exogenous biological renal support ameliorates renal pathology after ischemia reperfusion injury in elderly mice.
Abstract We established an exogenous biological renal support model through the generation of parabiotic mice. At 72 hours after ischemia reperfusion injury (IRI), the aged mice that received exogenous biological renal support showed significantly higher levels of renal cell proliferation and dedifferentiation, lower levels of renal tubular injury, improved renal function, and a lower mortality than those that did not receive exogenous biological renal support. Using the Quantibody Mouse Cytokine Antibody Array, we found that aged IRI mice that received exogenous biological renal support had an up-regulation of mu...
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Liu D, Yin Z, Huang Q, Ren Y, Li D, Wang L, Cui S, Zhang Y, Ning Y, Lun L, Cai G, Bai X, Sun X, Chen X Tags: Aging (Albany NY) Source Type: research

Synergistic interaction between bedtime and eating speed in predicting overweight and obesity in Chinese preschool-aged children.
This study aimed to examine the association of late bedtime and fast eating speed, both individually and interactively, in predicting overweight and obesity in Chinese preschool-aged children. This was a cross-sectional survey among children aged 3-6 years. Overweight and obesity is defined according to the WHO, IOTF, and China criteria, respectively. Total 1123 preschool-aged children were analyzed. After multivariable adjustment, late bedtime after 11:00 pm and fast eating speed increased the risk of overweight and obesity significantly under the WHO (odds ratio [OR]=1.92 and 1.37, 95% confidence interval [CI]: 1.31-2.80...
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Liu S, Zhang J, Ma J, Shang Y, Ma Y, Zhang X, Wang S, Yuan Y, Deng X, Niu W, Zhang Z Tags: Aging (Albany NY) Source Type: research

N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse.
In this study, we aimed to investigate the effects of N-acetyl-L-cysteine (NAC), a broadly used antioxidant, on oocyte quality in mouse post-ovulatory oocyte aging in vitro. NAC at 0.6mM concentration was added to culture medium (M2), and the quality of oocytes was analyzed at 6h, 12h, 18h and 24h of culture. We found that the frequency of spindle defects decreased in NAC-treated oocytes compared to those without NAC treatment. NAC treatment significantly decreased abnormal distribution of cortical granules (CGs) in oocytes during aging for 18h and 24h. Decreased intracellular reactive oxygen species (ROS) was also observe...
Source: Aging - April 12, 2019 Category: Biomedical Science Authors: Wang Y, Li L, Fan LH, Jing Y, Li J, Ouyang YC, Wang ZB, Hou Y, Sun QY Tags: Aging (Albany NY) Source Type: research

Autonomic dysfunction in neurological disorders.
PMID: 30969941 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 9, 2019 Category: Biomedical Science Authors: Xiong L, Leung TWH Tags: Aging (Albany NY) Source Type: research

Serum fatty acid binding protein 4 is positively associated with early stroke recurrence in nondiabetic ischemic stroke.
This study included the 206 acute ischemic stroke patients hospitalized in our institution. Stroke recurrence events were assessed at the 3-month follow-up. The median of FABP level was 22.6 (IQR, 17.9-31.6) ng/mL in patients with stroke recurrence (N=36), which was higher than in patients without stroke recurrence [16.9 (IQR, 11.8-21.4) ng/mL] (P
Source: Aging - April 9, 2019 Category: Biomedical Science Authors: Li B, Wu J, Jiang P, Li M, Liu Q, Cao Y, Wang S Tags: Aging (Albany NY) Source Type: research

Sex differences in the aging human heart: decreased sirtuins, pro-inflammatory shift and reduced anti-oxidative defense.
In conclusion, the present study revealed a female sex-specific downregulation of Sirt1 and Sirt3 in aged hearts, as well as a decline in mitochondrial anti-oxidative defense and a pro-inflammatory shift in old female hearts but not in male hearts. PMID: 30964749 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 8, 2019 Category: Biomedical Science Authors: Barcena de Arellano ML, Pozdniakova S, Kühl AA, Baczko I, Ladilov Y, Regitz-Zagrosek V Tags: Aging (Albany NY) Source Type: research

IgE, α-Gal and atherosclerosis.
IgE, α-Gal and atherosclerosis. Aging (Albany NY). 2019 Apr 05;: Authors: Wilson JM, McNamara CA, Platts-Mills TAE PMID: 30958794 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - April 5, 2019 Category: Biomedical Science Authors: Wilson JM, McNamara CA, Platts-Mills TAE Tags: Aging (Albany NY) Source Type: research

Ginsenoside Rb1 prevents MPTP-induced changes in hippocampal memory via regulation of the α-synuclein/PSD-95 pathway.
In this study, we found that Rb1 can prevent the spatial learning and memory deficits, increase long-term potentiation (LTP) and hippocampal glutamatergic transmission in the MPTP mouse model. The underlying neuroprotective mechanism of Rb1-improved synaptic plasticity involves Rb1 promoting hippocampal CA3 α-synuclein expression, restoring the glutamate in the CA3-schaffer collateral-CA1 pathway, and sequentially increasing postsynaptic density-95 (PSD-95) expression. Thus, we provide evidence that Rb1 modulates memory function, synaptic plasticity, and excitatory transmission via the trans-synaptic α-synuclei...
Source: Aging - April 4, 2019 Category: Biomedical Science Authors: Qu S, Meng X, Liu Y, Zhang X, Zhang Y Tags: Aging (Albany NY) Source Type: research

Elevation of circular RNA circ_0005230 facilitates cell growth and metastasis via sponging miR-1238 and miR-1299 in cholangiocarcinoma.
Abstract Cholangiocarcinoma (CCA) is a highly malignant carcinoma with high mortality rate worldwide. Emerging evidence indicates that aberrantly expressed circular RNAs (circRNAs) functions crucial roles in tumor progression. In this work, we focused on a novel circRNA, circ_0005230, in carcinogenesis and development of CCA. Circ_0005230 levels in CCA specimens and cells were measured by qRT-PCR. The clinical implication of circ_0005230 was analyzed by fisher's exact test. Gain/loss of-function assays were conducted to reveal the effects of circ_0005230 on the cell proliferation, apoptosis, migration and invasion...
Source: Aging - April 4, 2019 Category: Biomedical Science Authors: Xu Y, Yao Y, Liu Y, Wang Z, Hu Z, Su Z, Li C, Wang H, Jiang X, Kang P, Sun D, Zhong X, Cui Y Tags: Aging (Albany NY) Source Type: research

Long non-coding RNA CDKN2B-AS1 reduces inflammatory response and promotes cholesterol efflux in atherosclerosis by inhibiting ADAM10 expression.
CONCLUSION: Altogether, lncRNA CDKN2B-AS1 can inhibit the transcription of ADAM10 via DNMT1-mediated ADAM10 DNA methylation, consequently preventing inflammatory response of atherosclerosis and promoting cholesterol efflux. PMID: 30926762 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 29, 2019 Category: Biomedical Science Authors: Li H, Han S, Sun Q, Yao Y, Li S, Yuan C, Zhang B, Jing B, Wu J, Song Y, Wang H Tags: Aging (Albany NY) Source Type: research

DNA methylation in genes of longevity-regulating pathways: association with obesity and metabolic complications.
cute;nez JA Abstract Aging is the main risk factor for most chronic diseases. Epigenetic mechanisms, such as DNA methylation (DNAm) plays a pivotal role in the regulation of physiological responses that can vary along lifespan. The aim of this research was to analyze the association between leukocyte DNAm in genes involved in longevity and the occurrence of obesity and related metabolic alterations in an adult population. Subjects from the MENA cohort (n=474) were categorized according to age () and the presence of metabolic alterations: increased waist circumference, hypercholesterolemia, insulin resistance, and ...
Source: Aging - March 29, 2019 Category: Biomedical Science Authors: Salas-Pérez F, Ramos-Lopez O, Mansego ML, Milagro FI, Santos JL, Riezu-Boj JI, Martínez JA Tags: Aging (Albany NY) Source Type: research

Endothelial cells secreted endothelin-1 augments diabetic nephropathy via inducing extracellular matrix accumulation of mesangial cells in ETBR-/- mice.
In this study, STZ-diabetic ETBR-/- mice was characterized by increased serum creatinine and urinary albumin, enhanced glomerulosclerosis, and upregulated ET-1 expression compared with STZ-diabetic WT mice. In vitro, HG conditioned media (CM) of ETBR-/- GENs promoted mesangial cell proliferation and upregulated ECM-related proteins, and ET-1 knockout in GENs or inhibition of ET-1/ETAR in mesangial cell suppressed mesangial cell proliferation and collagen IV formation. In addition, ET-1 was over-expressed in ETBR-/- GENs and was regulated by NF-kapapB pathway. ET-1/ETBR suppressed NF-kappaB to modulate ET-1 in GENs. Further...
Source: Aging - March 29, 2019 Category: Biomedical Science Authors: Zou HH, Wang L, Zheng XX, Xu GS, Shen Y Tags: Aging (Albany NY) Source Type: research

SIRT6 participates in the quality control of aged oocytes via modulating telomere function.
Abstract It has been well recognized that oocyte quality declines in aging animals. However, to date, the underlying mechanism remains to be explored. In the present study, we report that oocytes and embryos from aged mice (42-45 weeks old) display the reduced expression of SIRT6 protein, accompanying with telomere shortening and DNA lesions. Moreover, we demonstrate that specific depletion of SIRT6 in oocytes induces dysfunctional telomeres and apoptosis of the resultant early embryos, leading to the developmental delay and cytoplasmic fragmentation. Importantly, we further find that overexpression of SIRT6 in ag...
Source: Aging - March 29, 2019 Category: Biomedical Science Authors: Ge J, Li C, Li C, Huang Z, Zeng J, Han L, Wang Q Tags: Aging (Albany NY) Source Type: research

Hepatic lipid droplet breakdown through lipolysis during hibernation in Chinese Soft-Shelled Turtle (Pelodiscus sinensis).
Abstract Hibernation is an adaptive survival strategy in response to cold and foodless winter. To determine the underlying mechanisms of seasonal adaptions, transcriptome sequencing studies have been conducted in bears, ground squirrels and bats. Despite advances in identifying differentially expressed genes involved in metabolism, the precise mechanisms of these physiological adaptions remain unclear. In the present study, we examined liver of Chinese Soft-Shelled Turtle (Pelodiscus sinensis) and found that the contents of lipid droplet (LD) and triglyceride (TG) were significantly decreased during hibernation. I...
Source: Aging - March 29, 2019 Category: Biomedical Science Authors: Huang Y, Chen H, Yang P, Bai X, Shi Y, Vistro WA, Tarique I, Haseeb A, Chen Q Tags: Aging (Albany NY) Source Type: research

Risk indexation and atrial fibrillation.
PMID: 30923257 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 28, 2019 Category: Biomedical Science Authors: Nguyen TH, Heresztyn T, Horowitz JD Tags: Aging (Albany NY) Source Type: research

Reactivation of microRNA-506 inhibits gastric carcinoma cell metastasis through ZEB2.
Abstract MicroRNAs (miRNAs) are frequently dysregulated in a variety of human cancers, including gastric carcinoma. To improve our understanding of the role of miRNAs in gastric carcinoma and potential identify novel biomarkers or therapeutic agents, we performed microarray analysis to identify differentially expressed miRNAs in gastric carcinoma, compared with paired non-cancerous gastric tissues. We identified significantly differentially expressed miRNAs in gastric carcinoma tissues, including miR-506. We validated the microarray results by quantitative reverse transcription polymerase chain reaction in 26 spec...
Source: Aging - March 28, 2019 Category: Biomedical Science Authors: Wang GJ, Jiao BP, Liu YJ, Li YR, Deng BB Tags: Aging (Albany NY) Source Type: research

Risk stratification in hypertrophic cardiomyopathy.
PMID: 30923259 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 28, 2019 Category: Biomedical Science Authors: Desai M, Mentias A Tags: Aging (Albany NY) Source Type: research

White light emitting diode induces autophagy in hippocampal neuron cells through GSK-3-mediated GR and ROR α pathways.
White light emitting diode induces autophagy in hippocampal neuron cells through GSK-3-mediated GR and RORα pathways. Aging (Albany NY). 2019 Mar 28;: Authors: Yang Y, Jia Y, Sun Q, Dong H, Zhao R Abstract Autophagy plays an important role in cell survival under diverse stress conditions. Here, we show that white LED light exposure for 24 h significantly activated autophagy-related genes and increased autophagosome formation in hippocampal neural cells (HT-22). Concurrently, the rhythmic pattern of clock-related gene expression was disrupted, which was associated with augmented expression of SIR...
Source: Aging - March 28, 2019 Category: Biomedical Science Authors: Yang Y, Jia Y, Sun Q, Dong H, Zhao R Tags: Aging (Albany NY) Source Type: research

Novel lacto-peptides improve cognitive decline.
PMID: 30923261 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 28, 2019 Category: Biomedical Science Authors: Ano Y, Nakayama H Tags: Aging (Albany NY) Source Type: research

Longitudinal assessment of health-span and pre-death morbidity in wild type Drosophila.
Abstract The increase in human life expectancy is accompanied by age-related cognitive and motor disability, thus raising the demand for strategies toward healthy aging. This requires understanding the biology of normal aging and late-life functional phenotypes. Genetic model organisms, such as Drosophila melanogaster, can help identifying evolutionary conserved mechanisms underlying aging. Longitudinal assessment of motor performance of more than 1000 individual flies revealed age-related motor performance decline and specific late-life motor disabilities. This allows defining heath- and ill-span and scoring late...
Source: Aging - March 27, 2019 Category: Biomedical Science Authors: Gaitanidis A, Dimitriadou A, Dowse H, Sanyal S, Duch C, Consoulas C Tags: Aging (Albany NY) Source Type: research

Saturated fatty acid-crystals activate NLRP3 inflammasome.
PMID: 30923255 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 26, 2019 Category: Biomedical Science Authors: Karasawa T, Takahashi M Tags: Aging (Albany NY) Source Type: research

H2S restores the cardioprotective effects of ischemic post-conditioning by upregulating HB-EGF/EGFR signaling.
Abstract Hydrogen sulfide (H2S) reduces ischemia/reperfusion (I/R) injury and apoptosis and restores the cardioprotective effects of ischemic post-conditioning (PC) in aged cardiomyocytes by inhibiting oxidative stress and endoplasmic reticulum stress and increasing autophagy. However, the mechanism is unclear. In the present study, we observed a loss of PC-mediated cardioprotection of aged cardiomyocytes. NaHS (a H2S donor) exerted significant protective effects against H/R-induced cell damage, apoptosis, production of cleaved caspase-3 and caspase-9, and release of cytochrome c. NaHS also reversed the H/R-induce...
Source: Aging - March 26, 2019 Category: Biomedical Science Authors: Zhang Y, Gao J, Sun W, Wen X, Xi Y, Wang Y, Wei C, Xu C, Li H Tags: Aging (Albany NY) Source Type: research

Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes.
Abstract Tubastatin A (TubA) is a highly selective histone deacetylase 6 (HDAC6) inhibitor. As expected, mouse germinal vesicle oocytes fail to extrude the first polar body following TubA treatment. However, a previous study demonstrated that homozygous Hdac6 knockout (KO) mice can be viable and fertile. Therefore, we asked whether TubA is indeed a specific inhibitor of HDAC6 activity. RNA-sequencing and in silico analysis demonstrated that the TubA-treated group presented significant changes in the expression of Hdac subfamily genes such as Hdac6, 10, and 11, and Sirtuin 2, 5, 6, and 7. Additionally, gene express...
Source: Aging - March 26, 2019 Category: Biomedical Science Authors: Choi YJ, Kang MH, Hong K, Kim JH Tags: Aging (Albany NY) Source Type: research

Non-infarcted myocardium bears the weight in CVD.
PMID: 30908271 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 25, 2019 Category: Biomedical Science Authors: Zhou H, Zainal H, Puntmann VO Tags: Aging (Albany NY) Source Type: research

From GWAS to new biology and treatments in CAD.
PMID: 30908272 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 25, 2019 Category: Biomedical Science Authors: Jones PD, Webb TR Tags: Aging (Albany NY) Source Type: research

Muscle-derived miR-34a increases with age in circulating extracellular vesicles and induces senescence of bone marrow stem cells.
Abstract Extracellular vesicles (EVs) are known to play important roles in cell-cell communication. Here we investigated the role of muscle-derived EVs and their microRNAs in the loss of bone stem cell populations with age. Aging in male and female C57BL6 mice was associated with a significant increase in expression of the senescence-associated microRNA miR-34a-5p (miR-34a) in skeletal muscle and in serum -derived EVs. Muscle-derived, alpha-sarcoglycan positive, EVs isolated from serum samples also showed a significant increase in miR-34a with age. EVs were isolated from conditioned medium of C2C12 mouse myoblasts...
Source: Aging - March 25, 2019 Category: Biomedical Science Authors: Fulzele S, Mendhe B, Khayrullin A, Johnson M, Kaiser H, Liu Y, Isales CM, Hamrick MW Tags: Aging (Albany NY) Source Type: research

Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging.
cci A Abstract The receptor for advanced glycation end-products (RAGE) recognizes several ligands involved in inflammatory diseases. Two circulating soluble isoforms exist: esRAGE derived from alternative splicing and cRAGE generated by the membrane-bound RAGE (FL-RAGE) proteolysis. Together, esRAGE and cRAGE constitute sRAGE and function as decoy receptors preventing FL-RAGE/ligands binding.We determined serum concentration of both, esRAGE and cRAGE, and their ligands AGEs, HMGB1 and S100A8/A9 in a healthy population of 169 subjects aged 20-90 years. cRAGE showed a negative (r=-0.375, P
Source: Aging - March 23, 2019 Category: Biomedical Science Authors: Scavello F, Zeni F, Tedesco CC, Mensà E, Veglia F, Procopio AD, Bonfigli AR, Olivieri F, Raucci A Tags: Aging (Albany NY) Source Type: research

Anesthesia and surgery induce cognitive dysfunction in elderly male mice: the role of gut microbiota.
In this study, we investigated the potential role of gut microbiota in POCD in aged male mice and attempted to determine whether alterations in gut microbiota would be helpful in the diagnosis of POCD. POCD and non-POCD mice were classified by hierarchical cluster analysis of behavioral results. Additionally, α- and β-diversity of gut microbiota showed a differential profile between the groups. In total, 24 gut bacteria were significantly altered in POCD mice compared with those in non-POCD mice, in which 13 gut bacteria were significantly correlated with escape latency in the Morris water maze test (MWMT). Rema...
Source: Aging - March 23, 2019 Category: Biomedical Science Authors: Zhan G, Hua D, Huang N, Wang Y, Li S, Zhou Z, Yang N, Jiang R, Zhu B, Yang L, Yu F, Xu H, Yang C, Luo A Tags: Aging (Albany NY) Source Type: research

Elevated FPR confers to radiochemoresistance and predicts clinical efficacy and outcome of metastatic colorectal cancer patients.
Abstract Association of chronic inflammation, primary tumor sidedness, adjuvant therapy and survival of metastatic colorectal cancer (mCRC) remains unclear. Circulating inflammatory cell, fibrinogen (Fib), albumin (Alb), pre-albumin (pAlb), Alb/Fib (AFR) and Fib/pAlb (FPR) were detected, and clinical outcome was obtained to determine the predictive, prognostic and monitoring roles of them in discovery and validation cohort. We found that elevated FPR, low AFR and poor survival was observed in right-sided mCRC comparing to the left-sided disease, elevated FPR harbored the highest areas under curve to independently ...
Source: Aging - March 21, 2019 Category: Biomedical Science Authors: Chen QG, Zhang L, Sun F, Li SQ, You XH, Jiang YH, Yang WM, Zhong QH, Wang XZ, Ying HQ Tags: Aging (Albany NY) Source Type: research

Circulating GDF11 levels are decreased with age but are unchanged with obesity and type 2 diabetes.
or J, Frühbeck G, Gómez-Ambrosi J Abstract Growth differentiation factor 11 (GDF11) is a member of the transforming growth factor β (TGFβ) superfamily which declines with age and exerts anti-aging regenerative effects in skeletal muscle in mice. However, recent data in humans and mice are conflicting casting doubts about its true functional actions. The aim of the present study was to compare the circulating concentrations of GDF11 in individuals of different ages as well as body weight and glycemic status. Serum concentrations of GDF11 were measured by ELISA in 319 subjects. There was a sign...
Source: Aging - March 21, 2019 Category: Biomedical Science Authors: Añón-Hidalgo J, Catalán V, Rodríguez A, Ramírez B, Silva C, Galofré JC, Salvador J, Frühbeck G, Gómez-Ambrosi J Tags: Aging (Albany NY) Source Type: research

A comparison of the mitochondrial proteome and lipidome in the mouse and long-lived Pipistrelle bats.
Abstract It is accepted that smaller mammals with higher metabolic rates have shorter lifespans. The very few species that do not follow these rules can give insights into interesting differences. The recorded maximum lifespans of bats are exceptional - over 40 years, compared with the laboratory mouse of 4 years. We investigated the differences in the biochemical composition of mitochondria between bat and mouse species. We used proteomics and ultra-high-performance liquid chromatography coupled with high resolution mass spectrometry lipidomics, to interrogate mitochondrial fractions prepared from Mus musculus an...
Source: Aging - March 19, 2019 Category: Biomedical Science Authors: Pollard AK, Ingram TL, Ortori CA, Shephard F, Brown M, Liddell S, Barrett DA, Chakrabarti L Tags: Aging (Albany NY) Source Type: research

Trends in the incidence of recurrent stroke at 5 years after the first-ever stroke in rural China: a population-based stroke surveillance from 1992 to 2017.
Abstract Recent data on the incidence and trends for recurrent strokes in China are scarce. We assessed the temporal trends in recurrent stroke incidence using in rural China. The age-standardized incidences of recurrent stroke, within 5 years of the incident stroke event, were estimated for 3 time periods: 1992-1998, 1999-2005, and 2006-2012. Among the 768 documented incident stroke cases, 26.3% of the patients experienced recurrent stroke within 5 years. The overall age-adjusted recurrent stroke incidence was 43.93 per 100,000 person-years (1992-2012). During the 2006-2012 period, the recurrent stroke incidence ...
Source: Aging - March 19, 2019 Category: Biomedical Science Authors: Zhao W, Wu J, Liu J, Wu Y, Ni J, Gu H, Tu J, Wang J, An Z, Ning X Tags: Aging (Albany NY) Source Type: research

Mending the failing heart.
PMID: 30886129 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 18, 2019 Category: Biomedical Science Authors: Wang X, Peter K Tags: Aging (Albany NY) Source Type: research

Cellular retinoic acid binding protein-II expression and its potential role in skin aging.
Abstract Skin aging is an intricate biological process consisting of intrinsic and extrinsic alterations of epidermal and dermal structures. Retinoids play an important role in epidermal cell growth and differentiation and are beneficial to counteract skin aging. Cellular retinoic acid binding protein-II (CRABP-II) selectively binds all trans-retinoic acid, the most active retinoid metabolite, contributing to regulate intracytoplasmic retinoid trafficking and keratinocyte differentiation. Immunohistochemistry revealed a reduced epidermal and dermal CRABP-II expression in aged human and mouse skin. To better clarif...
Source: Aging - March 18, 2019 Category: Biomedical Science Authors: Bielli A, Scioli MG, D'Amico F, Tarquini C, Agostinelli S, Costanza G, Doldo E, Campione E, Passeri D, Coniglione F, Orlandi A Tags: Aging (Albany NY) Source Type: research

Gene signatures and prognostic values of m6A regulators in clear cell renal cell carcinoma - a retrospective study using TCGA database.
This study aims to identify gene signatures and prognostic values of m6A regulators in ccRCC. In this study, a total of 528 ccRCC patients from TCGA database with sequencing and CNV data were included. Survival analysis was performed using log-rank tests and Cox regression model. The association between alteration of m6A regulators and clinicopathological characteristics was examined using chi-square test. The results showed that alteration of m6A regulators was associated with pathologic stage. Patients with any CNVs of the regulatory genes had worse OS and DFS than those with diploid genes. Moreover, deletion of m6A &quo...
Source: Aging - March 15, 2019 Category: Biomedical Science Authors: Zhou J, Wang J, Hong B, Ma K, Xie H, Li L, Zhang K, Zhou B, Cai L, Gong K Tags: Aging (Albany NY) Source Type: research

GABAergic astrocytes in Alzheimer's disease.
PMID: 30877782 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - March 15, 2019 Category: Biomedical Science Authors: Garaschuk O, Verkhratsky A Tags: Aging (Albany NY) Source Type: research

Delayed γH2AX foci disappearance in mammary epithelial cells from aged women reveals an age-associated DNA repair defect.
Delayed γH2AX foci disappearance in mammary epithelial cells from aged women reveals an age-associated DNA repair defect. Aging (Albany NY). 2019 Mar 14;11(5):1510-1523 Authors: Anglada T, Repullés J, Espinal A, LaBarge MA, Stampfer MR, Genescà A, Martín M Abstract Aging is a degenerative process in which genome instability plays a crucial role. To gain insight into the link between organismal aging and DNA repair capacity, we analyzed DNA double-strand break (DSB) resolution efficiency in human mammary epithelial cells from 12 healthy donors of young and old ages. The frequ...
Source: Aging - March 14, 2019 Category: Biomedical Science Authors: Anglada T, Repullés J, Espinal A, LaBarge MA, Stampfer MR, Genescà A, Martín M Tags: Aging (Albany NY) Source Type: research