Taurine suppresses ROS-dependent autophagy via activating Akt/mTOR signaling pathway in calcium oxalate crystals-induced renal tubular epithelial cell injury.
Abstract Oxidative stress and autophagy are the key promoters of calcium oxalate (CaOx) nephrolithiasis. Taurine is an antioxidant that plays a protective role in the pathogenesis of kidney disease. Previous studies found that taurine suppressed cellular oxidative stress, and inhibited autophagy activation. However, the effect of taurine on CaOx kidney stone formation remains unknown. In the present work, we explored the regulatory effects of taurine on CaOx crystals-induced HK-2 cell injury. Results showed that pretreatment with taurine significantly enhanced the viability of HK-2 cells and ameliorated kidney tis...
Source: Aging - September 15, 2020 Category: Biomedical Science Authors: Sun Y, Dai S, Tao J, Li Y, He Z, Liu Q, Zhao J, Deng Y, Kang J, Zhang X, Yang S, Liu Y Tags: Aging (Albany NY) Source Type: research

Long non-coding RNA Loc490 inhibits gastric cancer cell proliferation and metastasis by upregulating RNA-binding protein Quaking.
Abstract Gastric cancer (GC) is one of the most common malignant tumor types worldwide. Long non-coding RNAs (lncRNAs) have important epigenetic effects, including altering the proliferation and metastasis of malignant tumors. We used gene chip technology to search for lncRNAs that were differentially expressed in GC and metastatic lymph node tissues compared with adjacent normal tissues. The lncRNA Loc490 and the RNA-binding protein Quaking (QKI) were downregulated in GC tissues and lymph node metastases compared with normal tissues, and the levels of these two genes correlated positively with one another. Loc490...
Source: Aging - September 15, 2020 Category: Biomedical Science Authors: He Z, Duan Z, Chen L, Li B, Zhou Y Tags: Aging (Albany NY) Source Type: research

Human skeletal stem cell aging.
PMID: 32929053 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Ambrosi TH, Goodnough LH, Chan CKF Tags: Aging (Albany NY) Source Type: research

Identification of a cullin5-RING E3 ligase transcriptome signature in glioblastoma multiforme.
This study has thus identified a simple transcriptome signature for GBM prognosis. PMID: 32931454 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Zheng S, Li Z Tags: Aging (Albany NY) Source Type: research

Constructing a global transcriptional regulatory landscape for early non-small cell lung cancer to identify hub genes and key pathways.
In conclusion, by carrying out a series of in-depth analyses, hub genes and key pathways associated with early NSCLC were identified by a global transcriptional regulatory landscape. PMID: 32931455 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Bu J, Zhang P, Zhu K, Yan Y, Shi B, Wang J, Xu S Tags: Aging (Albany NY) Source Type: research

The protective effects of dehydrocostus lactone against TNF- α-induced degeneration of extracellular matrix (ECM) in SW1353 cells.
The protective effects of dehydrocostus lactone against TNF-α-induced degeneration of extracellular matrix (ECM) in SW1353 cells. Aging (Albany NY). 2020 Sep 14;12: Authors: Wang L, Yang M, Zhang C, Huang F Abstract Osteoarthritis is a common joint disease that disrupts the lives of millions of people worldwide. To date, a safe and reliable treatment has not yet been announced. Excessive production of pro-inflammatory cytokines such as TNF-α plays an important role in the pathological development of OA. Dehydrocostus lactone (DHC) is a kind of sesquiterpene isolated from medicinal plants t...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Wang L, Yang M, Zhang C, Huang F Tags: Aging (Albany NY) Source Type: research

Graphene oxide inhibits cell migration and invasion by destroying actin cytoskeleton in cervical cancer cells.
CONCLUSION: GO exhibited inhibitory effects on cell migration and invasion possibly by destroying actin cytoskeleton in Hela cells, which is a potential component of the Smad and JNK signalling pathways. METHODS: GO was prepared and chracterized by UV visible light absorption spectroscopy and atomic force microscopy. Hela cells were treated with Go at different dose levels. Then, in vitro cytotoxicity of GO was evaluated by the MTT assay, colony-forming assay and cell apoptosis assay. The inhibitory effects of GO on tumor cell migration and invasion as well as actin cytoskeleton were explored using Hela cells. PM...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Wang J, Wang P, He Y, Liu X, Wang S, Ma C, Tian X, Wang J, Wu X Tags: Aging (Albany NY) Source Type: research

Umbilical cord mesenchymal stem cells protect thymus structure and function in aged C57 mice by downregulating aging-related genes and upregulating autophagy- and anti-oxidative stress-related genes.
CONCLUSIONS: mUCMSCs inhibited senescence of the thymus and promoted structural and functional thymus regeneration by downregulating the senescence genes p53 and p16 and upregulating the SOD, Sirt1 and Sirt3 genes, but the mechanism requires further research. METHODS: C57 mice were obtained and met the requirements of thymic aging. mUCMSCs were infused via the tail vein at a dose of 1×107 cells/kg twice per week for 3 weeks. Six weeks after the last transplantation, the thymus was weighed, and the thymus-to-body weight ratio was calculated. The thymus tissue was stained with HE. PMID: 32924972 [PubMed - as ...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Pan XH, Lin QK, Yao X, Li ZA, Cai XM, Pang RQ, Ruan GP Tags: Aging (Albany NY) Source Type: research

miR-345-5p regulates adipogenesis via targeting VEGF-B.
In conclusion, our results demonstrate that miR-345-5p inhibits adipocyte differentiation via targeting vascular endothelial growth factor B. PMID: 32927430 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Liu X, He Y, Feng Z, Sheng J, Dong A, Zhang M, Cao L Tags: Aging (Albany NY) Source Type: research

Long non-coding RNA SNHG12 promotes immune escape of ovarian cancer cells through their crosstalk with M2 macrophages.
In this study, we identified a new lncRNA small nucleolar RNA host gene 12 (SNHG12) and investigated its role in tumor immune escape. We analyzed the expression levels of interlukin (IL)-6R and programmed death-ligand 1 (PD-L1) in 51 ovarian cancer and 20 normal specimens by immunohistochemistry. The correlation between SNHG12 and IL-6R in clinical ovarian cancer samples was identified by RT-qPCR. We then performed SNHG12 gain- and loss-function experiments in order to investigate its role in the regulation of immune escape and the crosstalk between miR-21 and IL-6. T cell proliferation was assessed by flow cytometry. In v...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Qian M, Ling W, Ruan Z Tags: Aging (Albany NY) Source Type: research

Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis.
Abstract The diabetes drug metformin has recently been shown to possess anti-cancer properties when used with other chemotherapeutic drugs. However, detailed mechanisms by which metformin improves cancer treatment are poorly understood. Here we provide evidence in HepG2 hepatocellular carcinoma cells that metformin sensitizes cisplatin-resistant HepG2 cells (HepG2/DDP) through increasing cellular glycolysis and suppressing Nrf2-dependent transcription. We show that metformin increases glucose uptake and enhances glucose metabolism through glycolytic pathway, resulting in elevated concentrations of intracellular NA...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Cai L, Jin X, Zhang J, Li L, Zhao J Tags: Aging (Albany NY) Source Type: research

Shikonin overcomes drug resistance and induces necroptosis by regulating the miR-92a-1-5p/MLKL axis in chronic myeloid leukemia.
Abstract Development of resistance to tyrosine kinase inhibitors (TKIs) targeting the BCR/ABL fusion protein represents a major challenge in the treatment of chronic myeloid leukemia (CML). Since apoptosis resistance is the fundamental mechanism impeding TKIs' therapeutic effects, alternative approaches that induce nonapoptotic cell death are being pursued to treat TKI-resistant CML. Induction of necroptosis, a distinct, caspase-independent form of programmed cell death, may be a valuable strategy in this respect. The present study shows that shikonin, an herbal compound used in traditional Chinese medicine, overc...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Huang X, Chen Z, Ni F, Ye X, Qian W Tags: Aging (Albany NY) Source Type: research

High hsa_circ_0020123 expression indicates poor progression to non-small cell lung cancer by regulating the miR-495/HOXC9 axis.
In this study, the results showed that hsa_circ_0020123 expression increased in both tumor tissues and NSCLC cells. A higher hsa_circ_0020123 expression also led to poor prognoses among NSCLC patients assayed via FISH. The data of FISH also confirmed that hsa_circ_0020123 primarily had a cytoplasmic location. Hsa_circ_0020123 knockdown caused a significant decrease in nude mouse xenograft growth. Bioinformatics analyses and dual luciferase reporter assays confirmed that hsa_circ_0020123 was an miR-495 sponge and that the HOXC9 gene was a miR-495 target. The miR-495 downregulation reversed cell migration and proliferation i...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Bi R, Wei W, Lu Y, Hu F, Yang X, Zhong Y, Meng L, Wang M, Jiang L, Xie X Tags: Aging (Albany NY) Source Type: research

BRD4/8/9 are prognostic biomarkers and associated with immune infiltrates in hepatocellular carcinoma.
Abstract Bromodomain (BRD)-containing proteins are a class of epigenetic readers with unique recognition for N-acetyl-lysine in histones and functions of gene transcription and chromatin modification, known to be critical in various cancers. However, little is known about the roles of distinct BRD-containing protein genes in hepatocellular carcinoma (HCC). Most recently, we investigated the transcriptional and survival data of BRD1, BRD2, BRD3, BRD4, BRD7, BRD8, BRD9 in HCC patients through ONCOMINE, UALCAN, Human Protein Atlas, GEPIA, cBioPortal, STRING, TIMER databases. BRD1/2/3/4/7/8/9 were over-expressed in HC...
Source: Aging - September 14, 2020 Category: Biomedical Science Authors: Chen YR, Ouyang SS, Chen YL, Li P, Xu HW, Zhu SL Tags: Aging (Albany NY) Source Type: research

LINC00160 mediates sunitinib resistance in renal cell carcinoma via SAA1 that is implicated in STAT3 activation and compound transportation.
In this study, we identified that LINC00160 was associated with sunitinib resistance in renal cell carcinoma. Resistant tumor cells highly expressed LINC00160 to recruit transcriptional factor TFAP2A, which bound to SAA1 promoter regions and activated its expression. On one hand, SAA1 linked to ABCB1 protein, which facilitated sunitinib cellular efflux and diminished drug accumulation. On the other hand, SAA1 stimulated JAK-STAT signaling pathways, which countered cellular survival inhibition from drug. All these regulatory networks were well organized and collaborated, thus promoting sunitinib resistance in renal cell car...
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Cheng G, Liu Y, Liu L, Ruan H, Cao Q, Song Z, Bao L, Xu T, Xiong Z, Liu J, Liu D, Liang H, Jiang G, Yang X, Yang H, Chen K, Zhang X Tags: Aging (Albany NY) Source Type: research

Two identified subsets of CD8 T cells in obstructed kidneys play different roles in inflammation and fibrosis.
Abstract Inflammation plays a crucial role in initiating renal fibrosis after injury. The infiltration of inflammatory cells, such as CD4+ T cells and macrophages, contributes to renal fibrosis following ureteric obstruction. However, the function of CD8+ T cells in obstructed kidneys remains unclear. Although CD8+ T cell depletion intensifies renal fibrosis by decreasing IFN-γ and increasing IL-4 in the kidneys, the change and role of CD8 T cell populations following environmental changes during renal fibrosis are largely unknown. Here, we identified two CD8 T cell subsets in mouse obstructed kidneys with u...
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Wang J, Tian J, Sun J, Gao M, Dong Y Tags: Aging (Albany NY) Source Type: research

Functional connectivity network estimation with an inter-similarity prior for mild cognitive impairment classification.
Abstract Functional connectivity network (FCN) analysis is an effective technique for modeling human brain patterns and diagnosing neurological disorders such as Alzheimer's disease (AD) and its early stage, Mild Cognitive Impairment. However, accurately estimating biologically meaningful and discriminative FCNs remains challenging due to the poor quality of functional magnetic resonance imaging (fMRI) data and our limited understanding of the human brain. Inspired by the inter-similarity nature of FCNs, similar regions of interest tend to share similar connection patterns. Here, we propose a functional brain netw...
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Li W, Xu X, Jiang W, Wang P, Gao X Tags: Aging (Albany NY) Source Type: research

Jinmaitong ameliorates diabetic peripheral neuropathy in streptozotocin-induced diabetic rats by modulating gut microbiota and neuregulin 1.
Abstract Jinmaitong (JMT), a compound prescription of traditional Chinese medicine, has long been used as a therapy for diabetic peripheral neuropathy (DPN). However, the neuroprotective mechanisms of JMT and its effect on gut microbiota remained unknown. Here, we examined the effects of JMT on behavior, pathomorphology and gut microbiota in streptozotocin (STZ)-induced DPN rats. Compared to distilled water administration, JMT reversed decreases in mechanical withdraw threshold and intraepidermal nerve fiber density, improved neurological morphology of sciatic nerves, increased serum neuregulin 1 (NRG1) level and ...
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Xie J, Song W, Liang X, Zhang Q, Shi Y, Liu W, Shi X Tags: Aging (Albany NY) Source Type: research

G-protein coupled estrogen receptor activation protects the viability of hyperoxia-treated primary murine retinal microglia by reducing ER stress.
In conclusion, our study demonstrates that GPER activation enhances the survival of hyperoxia-treated primary retinal microglia by reducing ER stress. Our study demonstrates the therapeutic potential of GPER agonists such as G-1 in the early phase of ROP. PMID: 32920550 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Li R, Wang Y, Chen P, Meng J, Zhang H Tags: Aging (Albany NY) Source Type: research

PGC-1 α and ERRα in patients with endometrial cancer: a translational study for predicting myometrial invasion.
CONCLUSIONS: PGC-1α and ERRα induce the epithelial-mesenchymal-transition therefore invasion and migration in endometrial cancer, and may be novel biomarkers to predict the risk of advanced myometrial invasion. METHODS: PGC-1α, ERRα, and vimentin expression was analyzed in tissue microarrays using immunohistochemistry. PGC-1α and ERRα expression in endometrial cancer cell lines was investigated using quantitative PCR and western blotting analyses after infection with lentivirus-mediated small interfering RNA (siRNA) targeting PGC-1α (siRNA-PGC-1α) or overexpressing ERR&a...
Source: Aging - September 13, 2020 Category: Biomedical Science Authors: Chen L, Mao X, Huang M, Lei H, Xue L, Sun P Tags: Aging (Albany NY) Source Type: research

Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice.
In this study, we prepared an R. meyeri anthocyanin extract and analyzed its effects on neural stem cell (NSC) senescence in vivo and in vitro. We isolated mouse NSCs and used cell counting kit-8 (CCK-8), cell cycle, reactive oxygen species (ROS), and immunofluorescence methods to analyze the anti-aging effects of R. meyeri anthocyanins as well as naringenin (Nar), which metabolic analysis revealed as an important flavonoid in R. meyeri anthocyanins. RNA-sequencing (RNA-seq) and enzyme-linked immuno sorbent assay (ELISA) methods were also used to investigate Nar-specific mechanisms of anti-aging. After R. meyeri anthocyani...
Source: Aging - September 12, 2020 Category: Biomedical Science Authors: Gao J, Wu Y, He D, Zhu X, Li H, Liu H, Liu H Tags: Aging (Albany NY) Source Type: research

Fecal microbiota transplantation alters the susceptibility of obese rats to type 2 diabetes mellitus.
In this study, we transplanted healthy or obese-T2DM intestinal microbiota to ZDF and LZ rats, and used 16S rRNA and targeted metabonomics to evaluate the directional effect of the microbiota on the susceptibility of obese rats to T2DM. The glycolipid metabolism phenotype could be changed bidirectionally in obese rats instead of in lean ones. One possible mechanism is that the microbiota and metabolites alter the structure of the intestinal tract, and improve insulin and leptin resistance through JAK2 / IRS / Akt pathway. It is worth noting that 7 genera, such as Lactobacillus, Clostridium and Roche, can regulate 15 metabo...
Source: Aging - September 12, 2020 Category: Biomedical Science Authors: Zhang L, Zhou W, Zhan L, Hou S, Zhao C, Bi T, Lu X Tags: Aging (Albany NY) Source Type: research

Identification and prognostic value of metabolism-related genes in gastric cancer.
Abstract Gastric cancer (GC) is one of the most commonly occurring cancers, and metabolism-related genes (MRGs) are associated with its development. Transcriptome data and the relevant clinical data were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases, and we identified 194 MRGs differentially expressed between GC and adjacent nontumor tissues. Through univariate Cox and lasso regression analyses we identified 13 potential prognostic differentially expressed MRGs (PDEMRGs). These PDEMRGs (CKMT2, ME1, GSTA2, ASAH1, GGT5, RDH12, NNMT, POLR1A, ACYP1, GLA, OPLAH, DCK, and POLD3) were used...
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Wen F, Huang J, Lu X, Huang W, Wang Y, Bai Y, Ruan S, Gu S, Chen X, Shu P Tags: Aging (Albany NY) Source Type: research

Identification of subtype-specific genes signature by WGCNA for prognostic prediction in diffuse type gastric cancer.
CONCLUSION: Thus, we constructed a reliable prognostic model for diffuse type gastric cancer, which should be beneficial for clinical therapeutic decision-making. PMID: 32915770 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Zhou Q, Zhou LQ, Li SH, Yuan YW, Liu L, Wang JL, Wu DZ, Wu Y, Xin L Tags: Aging (Albany NY) Source Type: research

A newborn with normal IgM and elevated IgG antibodies born to an asymptomatic infection mother with COVID-19.
We report a newborn with normal IgM and elevated IgG antibodies born to an asymptomatic infection mother with COVID-19. We assessed whether there was intrauterine vertical transmission potential of COVID-19. PMID: 32915771 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Gao W, Deng Z, Zeng L, Yang Y, Gong H, Liu J, Xiao H Tags: Aging (Albany NY) Source Type: research

Chloride intracellular channel protein 2: prognostic marker and correlation with PD-1/PD-L1 in breast cancer.
Abstract Immune checkpoint inhibition has emerged as an effective treatment for multiple solid tumors, including advanced-stage breast cancer (BC). During the past decade, the US Food and Drug Administration has approved a number of agents for immune checkpoint blockade (ICB). However, the limited data on monotherapy anti-tumor activity in BC underscores the need for robust predictive biomarker development. Here, we used weighted gene coexpression network analysis of genes differentially expressed between BC and normal tissue to identify genes coexpressed with programmed death-1 (PD-1) and its ligand (PD-L1). Tumo...
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Xu T, Wang Z, Dong M, Wu D, Liao S, Li X Tags: Aging (Albany NY) Source Type: research

Sirt6 is required for spermatogenesis in mice.
Abstract SIRT6, a nuclear protein, has been implicated in a number of essential cellular processes, such as the DNA damage response, metabolic homeostasis, inflammation, tumorigenesis and aging. However, the role of Sirt6 in the regulation of spermatogenesis is yet unknown. In the present study, we successfully generated Sirt6-/- mice on a C57BL6/ICR mixed background and found that some Sirt6-/- mice survived beyond eight weeks. We further revealed that spermatogenesis in Sirt6-/- mice was arrested at the elongated spermatid stage. Sirt6-/- male mice were completely infertile and had an increased number of apoptot...
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Wei H, Khawar MB, Tang W, Wang L, Wang L, Liu C, Jiang H, Li W Tags: Aging (Albany NY) Source Type: research

Physical performance and chronic kidney disease development in elderly adults: results from a nationwide cohort study.
Abstract Sarcopenia, which is characterized by muscle mass and physical performance, is closely associated with morbidities and mortality, especially among the elderly. However, the effect of physical performance on chronic kidney disease (CKD) development is not yet fully elucidated. A total of 30,871 adults aged 66 years with preserved renal function who underwent health screening examinations were evaluated. Physical performance was assessed using a 3-m timed up and go (TUG) test and the one-leg stand (OLS) test. The primary outcome was the development of CKD, defined as at least two consecutive measurements of...
Source: Aging - September 11, 2020 Category: Biomedical Science Authors: Joo YS, Jhee JH, Kim HW, Han SH, Yoo TH, Kang SW, Park JT Tags: Aging (Albany NY) Source Type: research

Discovering correlates of age-related decline in a healthy late-midlife male birth cohort.
Abstract Studies exploring age-related brain and cognitive change have identified substantial heterogeneity among individuals, but the underlying reasons for the differential trajectories remain largely unknown. We investigated cross-sectional and longitudinal associations between brain-imaging phenotypes (IDPs) and cognitive ability, and how these relations may be modified by common risk and protective factors. Participants were recruited from the 1953 Danish Male Birth Cohort (N=123), a longitudinal study of cognitive and brain ageing. Childhood IQ and socio-demographic factors are available for these participan...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Zarnani K, Smith SM, Alfaro-Almagro F, Fagerlund B, Lauritzen M, Rostrup E, Nichols TE Tags: Aging (Albany NY) Source Type: research

Profiling of circular RNAs in age-related cataract reveals circZNF292 as an antioxidant by sponging miR-23b-3p.
Abstract Age-related cataract (ARC) is one of the major causes of visual impairment and reversible blindness worldwide. Accumulating evidence has revealed that circular RNAs (circRNAs) are involved in multiple regulatory processes in various ocular diseases. However, the expression profile, regulatory roles, and underlying mechanisms of circRNAs in ARC remain largely unknown. Herein we deep-sequenced circRNAs of anterior lens capsules from normal and ARC lenses, and detected 23,787 candidate circRNAs. Of these, 466 were significantly differentially expressed, and a higher correlation in down-regulated circRNAs bet...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Liang S, Dou S, Li W, Huang Y Tags: Aging (Albany NY) Source Type: research

Relation between left atrial structure and lacunar infarction in patients with hypertension.
Abstract A lacunar infarction (LACI) can cause damage to the surrounding brain tissue and place an individual at greater risk for future major stroke. LACI is associated with hypertension and hypertension is associated with left atrial enlargement. It is important to identify a high-risk patient who is more vulnerable to suffering a LACI in hypertensive group. So, we studied whether left atrium size is an independent risk predictor for LACI in hypertensive patients. We performed cross-sectional analysis of 365 patients with hypertension at Shanghai Ninth People's Hospital from January 2016 to January 2017. The res...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Sun T, Xie T, Zhang A, Fan L, Xu Z, Chen X, Fan Z, Wang C Tags: Aging (Albany NY) Source Type: research

Male patients with TERT mutation may be more likely to benefit from immunotherapy, especially for melanoma.
Abstract Genomic mutation may be key factors for sex-biased disparities in cancer diagnosis, prognosis and prediction of treatment response. Current study has revealed that sex-based dimorphism on the efficacy of immune checkpoint inhibitors (ICIs) in various cancers and confirmed that male patients can benefit more from immunotherapy. However, only a subset of male patients responds well to ICIs. Therefore, biomarkers are desperately needed to identify the group of patients who may be more likely to benefit from ICIs. With the availability of the cBioPortal database, we identified that TERT mutation may serve as ...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Li J, Li Z, Zhang C, Zhang C, Wang H Tags: Aging (Albany NY) Source Type: research

Clinical course and risk factors for recurrence of positive SARS-CoV-2 RNA: a retrospective cohort study from Wuhan, China.
The objective of this study was to determine the clinical course and risk factors for patients showing recurrent SARS-CoV-2 RNA positivity. A total of 1087 COVID-19 patients confirmed by RT-PCR from February 24, 2020 to March 31, 2020 were retrospectively enrolled. Advanced age was significantly associated with mortality. In addition, 81 (7.6%) of the discharged patients tested positive for SARS-CoV-2 RNA during the isolation period. For patients with recurrent RT-PCR positivity, the median duration from illness onset to recurrence was 50 days. Multivariate regression analysis identified elevated serum IL-6, increased lymp...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Chen J, Xu X, Hu J, Chen Q, Xu F, Liang H, Liu N, Zhu H, Lan J, Zhou L, Xing J, Pu N, Cai Z Tags: Aging (Albany NY) Source Type: research

Long non-coding RNA DLEUI promotes papillary thyroid carcinoma progression by sponging miR-421 and increasing ROCK1 expression.
Abstract We investigated the role of long non-coding RNA DLEU1 (deleted in lymphocytic leukemia 1) in the progression of papillary thyroid carcinoma (PTC). DLEU1 levels were higher in PTC cell lines (BHP5-16, TPC-1,8505C, and SW1736) and patient tissues (n=54) than in a human thyroid follicular epithelial cell line (Nthy-ori3-1) or adjacent normal thyroid tissues. High DLEU1 expression correlated positively with lymph node metastasis and advanced clinical stages in PTC patients. Bioinformatics, dual luciferase reporter, and RNA pulldown assays confirmed that DLEU1 directly binds to miR-421. Moreover, bioinformatic...
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Li R, Wan T, Qu J, Yu Y, Zheng R Tags: Aging (Albany NY) Source Type: research

LNK deficiency decreases obesity-induced insulin resistance by regulating GLUT4 through the PI3K-Akt-AS160 pathway in adipose tissue.
In conclusion, LNK plays a pivotal role in adipose glucose transport by regulating insulin-mediated IRS1/PI3K/Akt/AS160 signaling. PMID: 32911464 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 10, 2020 Category: Biomedical Science Authors: Zhong X, Ke C, Cai Z, Wu H, Ye Y, Liang X, Yu L, Jiang S, Shen J, Wang L, Xie M, Wang G, Zhao X Tags: Aging (Albany NY) Source Type: research

Dynamic changes in vascular size and density in transgenic mice with Alzheimer's disease.
Abstract Alzheimer's disease (AD) is one of the most common neurodegenerative diseases. Here, we used vessel size imaging to investigate the specific microvascular changes and most susceptible brain regions during AD progression in an amyloid precursor protein 23 (APP23) transgenic AD mouse model. Using 9.4 Tesla magnetic resonance imaging (MRI), the values of microvascular density (Density), mean vessel diameter (mVD), and vessel size index (VSI) were compared between APP23 and wild-type (WT) mice at 3, 6, 9, 14, and 20 months of age. Our results demonstrate that in 20-month old APP23 and WT mice, the Density val...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Xu X, Meng T, Wen Q, Tao M, Wang P, Zhong K, Shen Y Tags: Aging (Albany NY) Source Type: research

Annotation of susceptibility SNPs associated with atrial fibrillation.
CONCLUSION: Our study suggests the relationship between genetic variants and possible targeted genes, and provides insight into potential genetic pathways underlying AF incidence and development. The results may provide an encyclopedia of AF susceptibility SNPs and shed light on the functional mechanisms of AF variants identified through genetic studies. METHODS: We summarized AF susceptibility SNPs identified by GWAS and candidate gene based association studies, and give a comprehensive functional annotation of all these AF susceptibility loci. by genomic annotation, microRNA binding prediction, promoter activity ana...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Xu C, Zhang R, Xia Y, Xiong L, Yang W, Wang P Tags: Aging (Albany NY) Source Type: research

Spermidine inhibits neurodegeneration and delays aging via the PINK1-PDR1-dependent mitophagy pathway in C. elegans.
Abstract Aging is the primary driver of various diseases, including common neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Currently there is no cure for AD and PD, and the development of novel drug candidates is demanding. Spermidine is a small anti-aging molecule with elimination of damaged mitochondria via the process of mitophagy identified as a molecular mechanism of action. Here, we show that spermidine inhibits memory loss in AD worms and improves behavioral performance, e.g., locomotor capacity, in a PD worm model, both via the PINK1-PDR1-dependent mitophagy pathwa...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Yang X, Zhang M, Dai Y, Sun Y, Aman Y, Xu Y, Yu P, Zheng Y, Yang J, Zhu X Tags: Aging (Albany NY) Source Type: research

High expression of UNC5B enhances tumor proliferation, increases metastasis, and worsens prognosis in breast cancer.
Abstract UNC-5 Homolog B (UNC5B) is a member of the dependence receptor family that regulates cell survival and apoptosis in a ligand-dependent manner. UNC5B plays an important role in the development of multiple cancers, including colorectal, bladder, and thyroid cancer. However, the exact expression pattern and mechanism of UNC5B in breast cancer have not been well elucidated. Here, we showed that UNC5B expression was significantly upregulated in breast cancer using bioinformatics analysis and experimental validation. High UNC5B expression was correlated with poor overall survival in breast cancer patients. UNC5...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Wu S, Guo X, Zhou J, Zhu X, Chen H, Zhang K, Lu Y, Chen Y Tags: Aging (Albany NY) Source Type: research

The association of healthy behaviors with cognitive health expectancy in Chinese older adults: a population-based cohort study.
CONCLUSION: Extra years of life gained by a healthy dietary pattern, mental activities, and social activities are free of cognitive impairment for both sexes across ages. The beneficial impact of individual and combined modifiable factors on cognitive health is most prominent in old-old. METHODS: Data come from The Chinese Longitudinal Healthy Longevity Survey, a population-based cohort study of 27,193 participants aged 65+ conducted between 2002 and 2014. Smoking status, alcohol consumption, dietary pattern, marital status, physical, mental, social, and productive activities were assessed at baseline. Cognitive statu...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Hou C, Lin Y, Zimmer Z, Fang X Tags: Aging (Albany NY) Source Type: research

Evidence that dysplasia related microRNAs in Barrett's esophagus target PD-L1 expression and contribute to the development of esophageal adenocarcinoma.
Abstract Esophageal adenocarcinoma (EAC) is the cancer arising from the esophagus, which frequently develop from Barrett's esophagus (BE). Extracellular vesicles (EVs), particularly exosomes, are nanosized vesicles of endosomal origin released from various types of cells that have been implicated in cancers. However, the significance of circulating exosomes during the progression of BE to EAC remains unknown. Sera exosmal microRNAs were profiled from 13 EAC and 12BE patients compared to 12 healthy controls. We found a substantial dysregulation of exosomal miRNA levels in BE compared to healthy control, and identif...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Xu J, Yin Z, Yang L, Wu F, Fan J, Huang Q, Jin Y, Yang G Tags: Aging (Albany NY) Source Type: research

Inactive bowel movement and stroke are associated with increased risks of mild cognitive impairment among community-living Singapore elderly.
Abstract Mild cognitive impairment (MCI), as a preclinical phase of dementia, provides an invaluable time window for intervention. Besides several proposed modifiable risk factors, the associations of MCI with dietary habits and bowel movement are not well clarified. We thus conducted a cross-sectional study of community-living Singapore elderly and focused on the relationship of clinically diagnosed MCI with dietary habits and bowel movement frequencies. The multiple logistic regression results showed that frequent (≥4 days per week) fruit consumption (P = 0.004), active (≥4 days per week) bowel movement wi...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Huang KY, Tang XY, Yang L, Zhang ZY, Ye KX, Shen QF, Wang X, Zhu XH, Huang XW, Lu GD, Feng L Tags: Aging (Albany NY) Source Type: research

Mitophagy impairment is involved in sevoflurane-induced cognitive dysfunction in aged rats.
Abstract Postoperative cognitive dysfunction (POCD) is frequently observed in elderly patients following anesthesia, but its pathophysiological mechanisms have not been fully elucidated. Sevoflurane was reported to repress autophagy in aged rat neurons; however, the role of mitophagy, which is crucial for the control of mitochondrial quality and neuronal health, in sevoflurane-induced POCD in aged rats remains undetermined. Therefore, this study investigated whether mitophagy impairment is involved in sevoflurane-induced cognitive dysfunction. We found sevoflurane treatment inhibited mitochondrial respiration and ...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Chen Y, Zhang P, Lin X, Zhang H, Miao J, Zhou Y, Chen G Tags: Aging (Albany NY) Source Type: research

Metabolic alterations in plasma from patients with familial and idiopathic Parkinson's disease.
Castillo A, Kroemer G, Bravo-San Pedro JM, Fuentes JM Abstract The research of new biomarkers for Parkinson's disease is essential for accurate and precocious diagnosis, as well as for the discovery of new potential disease mechanisms and drug targets. The main objective of this work was to identify metabolic changes that might serve as biomarkers for the diagnosis of this neurodegenerative disorder. For this, we profiled the plasma metabolome from mice with neurotoxin-induced Parkinson's disease as well as from patients with familial or sporadic Parkinson's disease. By using mass spectrometry technology, we analy...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Yakhine-Diop SMS, Morales-García JA, Niso-Santano M, González-Polo RA, Uribe-Carretero E, Martinez-Chacon G, Durand S, Maiuri MC, Aiastui A, Zulaica M, Ruíz-Martínez J, López de Munain A, Pérez-Tur J, Pérez-Castillo A, Kroemer G, Bravo-San Pedro JM Tags: Aging (Albany NY) Source Type: research

Retrospective study of gene signatures and prognostic value of m6A regulatory factor in non-small cell lung cancer using TCGA database and the verification of FTO.
In this study, a total of 1017 NSCLC patients from the cancer genome atlas (TCGA) database with copy number variation (CNV) data were included. Log-rank tests and Cox regression model were used for survival analysis. The relationship between m6A regulators and clinicopathological features was evaluated using the chi-square test. The alteration of m6A regulators were related to T stage. Patients with any CNVs of regulators genes had worse overall survival (OS) than those with diploid genes. The deletion of m6A writer genes was an independent risk factor for poor OS, and the effect synergized with that of copy number gain of...
Source: Aging - September 9, 2020 Category: Biomedical Science Authors: Shi H, Zhao J, Han L, Xu M, Wang K, Shi J, Dong Z Tags: Aging (Albany NY) Source Type: research

Knockdown of TXNDC9 induces apoptosis and autophagy in glioma and mediates cell differentiation by p53 activation.
In conclusion, the silencing of TXNDC9 induces apoptosis and autophagy in glioma and promotes cell differentiation by controlling p53 and may function as a new mechanism in glioma. PMID: 32897242 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 8, 2020 Category: Biomedical Science Authors: Zheng T, Chen K, Zhang X, Feng H, Shi Y, Liu L, Zhang J, Chen Y Tags: Aging (Albany NY) Source Type: research

DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells.
Abstract Colorectal cancer is a common health-threatening tumor within the gastrointestinal tract. The aim of this study was to test the biological role of DUSP4 in colorectal cancer cells. In our study, DUSP4 overexpression-treated HCT116 cells and DUSP4 knockdown-treated SW480 cells were selected to perform study. Quantitative real-time PCR test (qRT-PCR) and western blot were used to detect DUSP4 abundance in clinical tissues and six cell lines, as well as ubiquitin-related Smad4 degradation. Western blot, migration and invasion. were used to assess the relationships between DUSP4 and Smad4. Higher DUSP4 expres...
Source: Aging - September 7, 2020 Category: Biomedical Science Authors: Xu W, Chen B, Ke D, Chen X Tags: Aging (Albany NY) Source Type: research

Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes.
In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy. PMID: 32897243 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 7, 2020 Category: Biomedical Science Authors: Pan Y, Cheng A, Zhang X, Wang M, Chen S, Zhu D, Liu M, Zhao X, Yang Q, Wu Y, Huang J, Zhang S, Mao S, Ou X, Gao Q, Yu Y, Liu Y, Zhang L, Yin Z, Jing B, Tian B, Pan L, Rehman MU, Chen X, Jia R Tags: Aging (Albany NY) Source Type: research

Identification of cardiovascular health gene variants related to longevity in a Chinese population.
Abstract Cardiovascular disease (CVD) is one of the most important causes of human death, but no attention has been paid to cardiovascular health genes related to healthy longevity. Therefore, we developed a cohort study to explore such genes in healthy, long-lived Chinese subjects. A total of 13275 healthy elderly people were enrolled, including 5107 healthy long-lived individuals and 8168 age-matched control individuals with low CVD risk. Using a combination of whole-exome sequencing (WES) and genome-wide association studies (GWAS), we identified 2 genetic variants (TFPI rs7586970 T, p=0.013, OR=1.100. ADAMTS7 r...
Source: Aging - September 7, 2020 Category: Biomedical Science Authors: Zhang L, Bai C, Nie C, Zhu X, Yuan H, Sun L, Zhou Q, Li X, Xian X, Yang F, Pang G, Lv Y, Ni X, Hu C, Yang Z Tags: Aging (Albany NY) Source Type: research

HOXC10 promotes tumour metastasis by regulating the EMT-related gene Slug in ovarian cancer.
This study provides the first evidence that HOXC10 promotes ovarian cancer metastasis by regulating the transcription of the EMT-related gene Slug. Moreover, we found that HOXC10 is regulated by miR-222-3p. These data highlight the crucial role of HOXC10 in enhancing ovarian cancer metastasis and may provide a therapeutic target for ovarian cancer. PMID: 32897245 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - September 7, 2020 Category: Biomedical Science Authors: Peng Y, Li Y, Li Y, Wu A, Fan L, Huang W, Fu C, Deng Z, Wang K, Zhang Y, Shu G, Yin G Tags: Aging (Albany NY) Source Type: research