Prognostic and immune infiltration signatures of proteasome 26S subunit, non-ATPase (PSMD) family genes in breast cancer patients
Aging (Albany NY). 2021 Nov 28;13(undefined). doi: 10.18632/aging.203722. Online ahead of print.ABSTRACTThe complexity of breast cancer includes many interacting biological processes that make it difficult to find appropriate therapeutic treatments. Therefore, identifying potential diagnostic and prognostic biomarkers is urgently needed. Previous studies demonstrated that 26S proteasome delta subunit, non-ATPase (PSMD) family members significantly contribute to the degradation of damaged, misfolded, abnormal, and foreign proteins. However, transcriptional expressions of PSMD family genes in breast cancer still remain large...
Source: Aging - November 28, 2021 Category: Biomedical Science Authors: Do Thi Minh Xuan Chung-Che Wu Tzu-Jen Kao Hoang Dang Khoa Ta Gangga Anuraga Vivin Andriani Muhammad Athoillah Chung-Chieh Chiao Yung-Fu Wu Kuen-Haur Lee Chih-Yang Wang Jian-Ying Chuang Source Type: research

A scoring model based on ferroptosis genes for prognosis and immunotherapy response prediction and tumor microenvironment evaluation in liver hepatocellular carcinoma
Aging (Albany NY). 2021 Nov 28;13(undefined). doi: 10.18632/aging.203721. Online ahead of print.ABSTRACTFerroptosis is a type of iron-dependent programmed cell death. Ferroptosis inducers have been shown to have a great potential for cancer therapy. We aimed to generate a risk scoring model based on ferroptosis-related genes (FRGs) and validate its predictive performances in overall survival (OS) prediction and immunotherapy efficacy evaluation in liver hepatocellular carcinoma (LIHC). Differential and Univariate Cox regression analyses were applied to analyze RNA-seq data of LIHC samples from TCGA and GEO databases to ide...
Source: Aging - November 28, 2021 Category: Biomedical Science Authors: Lei Gao Juan Xue Xiaomin Liu Lei Cao Ruifang Wang Liangliang Lei Source Type: research

Progressive reorganization of mitochondrial apparatus in aging skeletal muscle of naked mole rats ( < em > Heterocephalus glaber) < /em > as revealed by electron microscopy: potential role in continual maintenance of muscle activity
Aging (Albany NY). 2021 Nov 28;13(undefined). doi: 10.18632/aging.203720. Online ahead of print.ABSTRACTThe authors examined the ultrastructure of mitochondrial apparatus of skeletal muscles of naked mole rats (Heterocephalus glaber) from the age of 6 months to 11 years. The obtained results have demonstrated that the mitochondria in skeletal muscles of naked mole rats aged below 5 years is not well-developed and represented by few separate small mitochondria. Mitochondrial reticulum is absent. Starting from the age of 5 years, a powerful mitochondrial structure are develop. By the age of 11 years, it become obvious that t...
Source: Aging - November 28, 2021 Category: Biomedical Science Authors: Valeriya Vays Irina Vangely Chupalav Eldarov Susanne Holtze Thomas Hildebrandt Lora Bakeeva Vladimir Skulachev Source Type: research

Genomic mutation features identify distinct BRCA-associated mutation characteristics in endometrioid carcinoma and endometrioid ovarian carcinoma
Aging (Albany NY). 2021 Nov 27;13(undefined). doi: 10.18632/aging.203710. Online ahead of print.ABSTRACTAlthough endometrioid carcinoma (EC) and endometrioid ovarian carcinoma (EnOC) display similar pathological features, their molecular characteristics remain to be determined. Somatic mutation data from 2777 EC, 423 EnOC, and 57 endometriosis patients from the Catalogue of Somatic Mutations in Cancer (COSMIC) dataset were analyzed and showed similar profiles with different mutation frequencies among them. By using 275 overlapping mutated genes, EC was clustered into two groups with different disease outcomes and different...
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Canhui Cao Ruidi Yu Wenjian Gong Dan Liu Xiaoxue Zhang Yong Fang Yu Xia Wei Zhang Qinglei Gao Source Type: research

Excluding embryos with two novel mutations in < em > FREM2 < /em > gene by the next-generation sequencing-based single nucleotide polymorphism haplotyping
In conclusion, we first reported two novel mutations in the FREM2 gene associated with the risk of Fraser syndrome. Moreover, we described a next-generation sequencing-based single nucleotide polymorphism haplotyping method to select the 'right' embryos from patients with Fraser syndrome for in vitro fertilization and embryo transfer treatment.PMID:34837691 | DOI:10.18632/aging.203715 (Source: Aging)
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Yao Zhou Xiaohui Yang Zheng Liu Yu Zhang Huaye Chen Yongfang Zhang Yuxin Hu Yanlin Ma Qi Li Source Type: research

Identification of pyroptosis-related signature for cervical cancer predicting prognosis
This study focuses on the development of a pyroptosis-associated prognostic signature for CC using integrated bioinformatics to delineate the relationships among the signature, tumor microenvironment, and immune response of the patients. In this respect, we identified a prognostic signature that depends on eight pyroptosis-related genes (PRGs) that designate with better prognostic survival in the low-risk group (P
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Cankun Zhou Chaomei Li Yuhua Zheng Xiaochun Liu Source Type: research

Detection of genomic structure variants associated with wrinkled skin in Xiang pig by next generation sequencing
Aging (Albany NY). 2021 Nov 27;13(undefined). doi: 10.18632/aging.203711. Online ahead of print.ABSTRACTWrinkling is prominent manifestation of aging skin. A mutant phenotype characterized by systemic wrinkles and thickened skin was discovered in Xiang pig populations with incidence about 1-3%. The feature in histological structure was epidermal hyperplasia and thickening, collagen fibers disorder. To uncover genetic mechanisms for the mutant phenotype of Xiang pigs with systemic wrinkle (WXP), a genome-wide of structural variations (SVs) in WXP was described by next generation resequencing, taking Xiang pigs (XP) and Euro...
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Liu Xiaoli Hu Fengbin Huang Shihui Niu Xi Li Sheng Wang Zhou Ran Xueqin Wang Jiafu Source Type: research

Bone morphogenetic protein 9 enhances osteogenic and angiogenic responses of human amniotic mesenchymal stem cells cocultured with umbilical vein endothelial cells through the PI3K/AKT/m-TOR signaling pathway
CONCLUSIONS: BMP9-enhanced osteoblastic and angiogenic differentiation in cocultivation with hAMSCs and HUVECs in vitro and in vivo also provide a chance to harness the BMP9-regulated coordinated effect between osteogenic and angiogenic pathways through BMP/Smad and PI3K/AKT/m-TOR signalings.MATERIALS AND METHODS: The ALP and Alizarin Red S staining assay to determine the effects of osteoblastic differentiation. RT-qPCR and western blot was measured the expression of angiogenesis-related factors. Ectopic bone formation was established and retrieved bony masses were subjected to histochemical staining. The angiogenesis abil...
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Ziming Liu Yuwan Li Jianye Yang Jiaxing Huang Changqi Luo Jun Zhang Wenqiang Yan Yingfang Ao Source Type: research

Long noncoding RNA NEAT1 inhibits the acetylation of PTEN through the miR-524-5p /HDAC1 axis to promote the proliferation and invasion of laryngeal cancer cells
Aging (Albany NY). 2021 Nov 27;13(undefined). doi: 10.18632/aging.203719. Online ahead of print.ABSTRACTLong noncoding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) is abnormally expressed in numerous tumors and functions as an oncogene, but the role of NEAT1 in laryngocarcinoma is largely unknown. Our study validated that NEAT1 expression was markedly upregulated in laryngocarcinoma tissues and cells. Downregulation of NEAT1 dramatically suppressed cell proliferation and invasion through inhibiting miR-524-5p expression. Additionally, NEAT1 overexpression promoted cell growth and metastasis, while overexpre...
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Jiajia Zhang Ping Wang Yanli Cui Source Type: research

Inhibition of Fam114A1 protects melanocytes from apoptosis through higher RACK1 expression
In this study, we investigated the effect of Fam114A1 on the biological activity of melanocytes. We found that the expression of Fam114A1 in vitiligo melanocytes (MCV-L, MCV-N, PI3V) was higher than that in normal melanocytes, and the biological function of melanocytes was significantly affected when the Fam114A1 gene was silenced. Inhibition of Fam114A1 increased proliferation, migration, and melanin synthesis proteins, decreased apoptosis, while its overexpression reversed this process. Mechanistically, we discovered that RACK1 is a target protein of Fam114A1 and that RACK1 can be negatively regulated by Fam114A1. Furthe...
Source: Aging - November 27, 2021 Category: Biomedical Science Authors: Miaoni Zhou Fuquan Lin Xingang Wu Zhuyi Ping Wen Xu Rong Jin Aie Xu Source Type: research

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling
Aging (Albany NY). 2021 Nov 25;13(undefined). doi: 10.18632/aging.203713. Online ahead of print.ABSTRACTPancreatic cancer is an extremely malignant digestive tract tumor. With the increase of chemotherapeutic resistance of pancreatic cancer, clinical treatment is in a dilemma. Hence, it is pivotal to design an effective drug for treating individuals with pancreatic cancer. Fisetin extracted from vegetables, as well as fruits was explored to possess antioxidant, anti-cancer, anti-inflammatory along with anti-microbial properties. Nonetheless, there is limited research focusing on the utility of fisetin as an inhibitor of pa...
Source: Aging - November 25, 2021 Category: Biomedical Science Authors: Yanyi Xiao Yilong Liu Zhiwei Gao Xian Li Min Weng Chenghao Shi Cheng Wang Linxiao Sun Source Type: research

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling
Aging (Albany NY). 2021 Nov 25;13(undefined). doi: 10.18632/aging.203713. Online ahead of print.ABSTRACTPancreatic cancer is an extremely malignant digestive tract tumor. With the increase of chemotherapeutic resistance of pancreatic cancer, clinical treatment is in a dilemma. Hence, it is pivotal to design an effective drug for treating individuals with pancreatic cancer. Fisetin extracted from vegetables, as well as fruits was explored to possess antioxidant, anti-cancer, anti-inflammatory along with anti-microbial properties. Nonetheless, there is limited research focusing on the utility of fisetin as an inhibitor of pa...
Source: Aging - November 25, 2021 Category: Biomedical Science Authors: Yanyi Xiao Yilong Liu Zhiwei Gao Xian Li Min Weng Chenghao Shi Cheng Wang Linxiao Sun Source Type: research

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling
Aging (Albany NY). 2021 Nov 25;13(undefined). doi: 10.18632/aging.203713. Online ahead of print.ABSTRACTPancreatic cancer is an extremely malignant digestive tract tumor. With the increase of chemotherapeutic resistance of pancreatic cancer, clinical treatment is in a dilemma. Hence, it is pivotal to design an effective drug for treating individuals with pancreatic cancer. Fisetin extracted from vegetables, as well as fruits was explored to possess antioxidant, anti-cancer, anti-inflammatory along with anti-microbial properties. Nonetheless, there is limited research focusing on the utility of fisetin as an inhibitor of pa...
Source: Aging - November 25, 2021 Category: Biomedical Science Authors: Yanyi Xiao Yilong Liu Zhiwei Gao Xian Li Min Weng Chenghao Shi Cheng Wang Linxiao Sun Source Type: research

BMAL1 may be involved in angiogenesis and peritumoral cerebral edema of human glioma by regulating VEGF and ANG2
Aging (Albany NY). 2021 Nov 23;13(undefined). doi: 10.18632/aging.203708. Online ahead of print.ABSTRACTThe rhythm gene BMAL1 (Brain and Muscle ARNT-Like 1) may play an important role in glioma tolerance for anti-angiogenesis therapy. In humans with glioma of different pathological grades, BMAL1 expression was significantly different, and the expression of ANG2 (Angiopoietin 2) and VEGF (Vascular endothelial growth factor) was positively correlated with the expression of BMAL1. Additionally, BMAL1 expression is positively correlated with the microvascular density and peritumoral edema of glioma. According to in vitro exper...
Source: Aging - November 24, 2021 Category: Biomedical Science Authors: Fan Wang CaiYan Li Fei Han LvAn Chen Ling Zhu Source Type: research

Hyperbaric oxygen therapy induces transcriptome changes in elderly: a prospective trial
In conclusion, for the first time in humans, the study provides direct evidence of HBOT is associated with transcriptome changes in whole-blood samples. Our results demonstrate significant changes in gene expression of normal aging population.PMID:34818212 | DOI:10.18632/aging.203709 (Source: Aging)
Source: Aging - November 24, 2021 Category: Biomedical Science Authors: Amir Hadanny Relly Forer Dina Volodarsky Malka Daniel-Kotovsky Merav Catalogna Yonatan Zemel Yair Bechor Shai Efrati Source Type: research

BMAL1 may be involved in angiogenesis and peritumoral cerebral edema of human glioma by regulating VEGF and ANG2
Aging (Albany NY). 2021 Nov 23;13(undefined). doi: 10.18632/aging.203708. Online ahead of print.ABSTRACTThe rhythm gene BMAL1 (Brain and Muscle ARNT-Like 1) may play an important role in glioma tolerance for anti-angiogenesis therapy. In humans with glioma of different pathological grades, BMAL1 expression was significantly different, and the expression of ANG2 (Angiopoietin 2) and VEGF (Vascular endothelial growth factor) was positively correlated with the expression of BMAL1. Additionally, BMAL1 expression is positively correlated with the microvascular density and peritumoral edema of glioma. According to in vitro exper...
Source: Aging - November 24, 2021 Category: Biomedical Science Authors: Fan Wang CaiYan Li Fei Han LvAn Chen Ling Zhu Source Type: research

Identification and replication of novel genetic variants of ABO gene to reduce the incidence of diseases and promote longevity by modulating lipid homeostasis
In conclusion, the association between the identified longevity-associated ABO variants and better health lipid profile was elucidated, thus the findings can help in maintaining normal lipid metabolic phenotypes in the longevity population.PMID:34812738 | DOI:10.18632/aging.203700 (Source: Aging)
Source: Aging - November 23, 2021 Category: Biomedical Science Authors: Xiaolin Ni Chen Bai Chao Nie Liping Qi Yifang Liu Huiping Yuan Xiaoquan Zhu Liang Sun Qi Zhou Yan Li Hefu Zhen Huabing Su Rongqiao Li Rushu Lan Guofang Pang Yuan Lv Wei Zhang Fan Yang Yao Yao Chen Chen Zhaoping Wang Danni Gao Nan Zhang Shenqi Zhang Li Zha Source Type: research

Atorvastatin combined with dexamethasone promote hematoma absorption in an optimized rat model of chronic subdural hematoma
In this study, we aimed to investigate the effect of pharmacotherapy in an optimized rat model of chronic subdural hematoma. Rat model of chronic subdural hematoma via a bEnd.3 cell and Matrigel mix was established and dynamic changes in different drug treatment groups were tested. The hematoma gradually increased, peaked on the fifth day (263.8±52.85 μl), and was completely absorbed in two weeks. Notably, Kruppelle-like factor 2 expression was significantly decreased with increasing hematoma volume, and then increased in the repair period. The expression of IL-10 was increased and peaked on 7 days, and then decr...
Source: Aging - November 23, 2021 Category: Biomedical Science Authors: Dong Wang Yueshan Fan Jun Ma Chuang Gao Xuanhui Liu Zilong Zhao Huijie Wei Guili Yang Jinhao Huang Rongcai Jiang Jianning Zhang Source Type: research

PPAR α agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway
CONCLUSION: PPARα agonist PEA can relieve SCI in rats by inhibiting inflammatory responses and oxidative stress, which may involve a mechanism associated with the activation of Nrf2/HO-1 via the Raf-1/MEK/ERK pathway.PMID:34799468 | DOI:10.18632/aging.203699 (Source: Aging)
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Haocong Zhang Dulei Xiang Xinwei Liu Liangbi Xiang Source Type: research

Construction of a genome instability-derived lncRNA based risk scoring system for the prognosis of hepatocellular carcinoma
Aging (Albany NY). 2021 Nov 18;13(undefined). doi: 10.18632/aging.203698. Online ahead of print.ABSTRACTEmerging evidence revealed the critical roles of long non-coding RNAs (lncRNAs) in maintaining genomic instability. However, genome instability-associated lncRNAs (GILncRNAs) and their performance in clinical prognostic significance in hepatocellular carcinoma (HCC) are rarely reported. Our study constructed a computational framework integrating somatic mutation information and lncRNA expression profiles of HCC genome and we identified 88 GILncRNAs of HCC. Function enrichment analysis revealed that GILncRNAs were involve...
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Dan-Ping Huang Mian-Mian Liao Jing-Jing Tong Wei-Qu Yuan De-Ti Peng Jian-Ping Lai Yi-Hao Zeng Yi-Jun Qiu Guang-Dong Tong Source Type: research

PPAR α agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway
CONCLUSION: PPARα agonist PEA can relieve SCI in rats by inhibiting inflammatory responses and oxidative stress, which may involve a mechanism associated with the activation of Nrf2/HO-1 via the Raf-1/MEK/ERK pathway.PMID:34799468 | DOI:10.18632/aging.203699 (Source: Aging)
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Haocong Zhang Dulei Xiang Xinwei Liu Liangbi Xiang Source Type: research

Construction of a genome instability-derived lncRNA based risk scoring system for the prognosis of hepatocellular carcinoma
Aging (Albany NY). 2021 Nov 18;13(undefined). doi: 10.18632/aging.203698. Online ahead of print.ABSTRACTEmerging evidence revealed the critical roles of long non-coding RNAs (lncRNAs) in maintaining genomic instability. However, genome instability-associated lncRNAs (GILncRNAs) and their performance in clinical prognostic significance in hepatocellular carcinoma (HCC) are rarely reported. Our study constructed a computational framework integrating somatic mutation information and lncRNA expression profiles of HCC genome and we identified 88 GILncRNAs of HCC. Function enrichment analysis revealed that GILncRNAs were involve...
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Dan-Ping Huang Mian-Mian Liao Jing-Jing Tong Wei-Qu Yuan De-Ti Peng Jian-Ping Lai Yi-Hao Zeng Yi-Jun Qiu Guang-Dong Tong Source Type: research

PPAR α agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway
CONCLUSION: PPARα agonist PEA can relieve SCI in rats by inhibiting inflammatory responses and oxidative stress, which may involve a mechanism associated with the activation of Nrf2/HO-1 via the Raf-1/MEK/ERK pathway.PMID:34799468 | DOI:10.18632/aging.203699 (Source: Aging)
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Haocong Zhang Dulei Xiang Xinwei Liu Liangbi Xiang Source Type: research

Construction of a genome instability-derived lncRNA based risk scoring system for the prognosis of hepatocellular carcinoma
Aging (Albany NY). 2021 Nov 18;13(undefined). doi: 10.18632/aging.203698. Online ahead of print.ABSTRACTEmerging evidence revealed the critical roles of long non-coding RNAs (lncRNAs) in maintaining genomic instability. However, genome instability-associated lncRNAs (GILncRNAs) and their performance in clinical prognostic significance in hepatocellular carcinoma (HCC) are rarely reported. Our study constructed a computational framework integrating somatic mutation information and lncRNA expression profiles of HCC genome and we identified 88 GILncRNAs of HCC. Function enrichment analysis revealed that GILncRNAs were involve...
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Dan-Ping Huang Mian-Mian Liao Jing-Jing Tong Wei-Qu Yuan De-Ti Peng Jian-Ping Lai Yi-Hao Zeng Yi-Jun Qiu Guang-Dong Tong Source Type: research

PPAR α agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway
CONCLUSION: PPARα agonist PEA can relieve SCI in rats by inhibiting inflammatory responses and oxidative stress, which may involve a mechanism associated with the activation of Nrf2/HO-1 via the Raf-1/MEK/ERK pathway.PMID:34799468 | DOI:10.18632/aging.203699 (Source: Aging)
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Haocong Zhang Dulei Xiang Xinwei Liu Liangbi Xiang Source Type: research

Construction of a genome instability-derived lncRNA based risk scoring system for the prognosis of hepatocellular carcinoma
Aging (Albany NY). 2021 Nov 18;13(undefined). doi: 10.18632/aging.203698. Online ahead of print.ABSTRACTEmerging evidence revealed the critical roles of long non-coding RNAs (lncRNAs) in maintaining genomic instability. However, genome instability-associated lncRNAs (GILncRNAs) and their performance in clinical prognostic significance in hepatocellular carcinoma (HCC) are rarely reported. Our study constructed a computational framework integrating somatic mutation information and lncRNA expression profiles of HCC genome and we identified 88 GILncRNAs of HCC. Function enrichment analysis revealed that GILncRNAs were involve...
Source: Aging - November 20, 2021 Category: Biomedical Science Authors: Dan-Ping Huang Mian-Mian Liao Jing-Jing Tong Wei-Qu Yuan De-Ti Peng Jian-Ping Lai Yi-Hao Zeng Yi-Jun Qiu Guang-Dong Tong Source Type: research

< em > BMPR2 < /em > promoter methylation and its expression in valvular heart disease complicated with pulmonary artery hypertension
In this study, we found DNA methylation was increased at the promoter of BMPR2 gene in the VHD patients compared with the healthy controls. This finding was confirmed by an independent cohort study of VHD patients and healthy controls. In addition, BMPR2 mRNA levels were reduced in the plasma of the VHD patients. There is strong correlation between BMPR2 promoter DNA methylation and the severity of VHD. Indeed, we found that both BMPR2 promoter DNA methylation and BMPR2 mRNA levels in the plasma are good biomarkers of VHD by themselves, with the respective AUC value of 0.879 and 0.725, respectively. When they were used in ...
Source: Aging - November 18, 2021 Category: Biomedical Science Authors: Ni Li Linwen Zhu Caimin Zhu Hua Zhou Dawei Zheng Guodong Xu Huoshun Shi Jianqing Gao Albert Jiarui Li Zhaoyang Wang Lebo Sun Xiajun Li Guofeng Shao Source Type: research

< em > BMPR2 < /em > promoter methylation and its expression in valvular heart disease complicated with pulmonary artery hypertension
In this study, we found DNA methylation was increased at the promoter of BMPR2 gene in the VHD patients compared with the healthy controls. This finding was confirmed by an independent cohort study of VHD patients and healthy controls. In addition, BMPR2 mRNA levels were reduced in the plasma of the VHD patients. There is strong correlation between BMPR2 promoter DNA methylation and the severity of VHD. Indeed, we found that both BMPR2 promoter DNA methylation and BMPR2 mRNA levels in the plasma are good biomarkers of VHD by themselves, with the respective AUC value of 0.879 and 0.725, respectively. When they were used in ...
Source: Aging - November 18, 2021 Category: Biomedical Science Authors: Ni Li Linwen Zhu Caimin Zhu Hua Zhou Dawei Zheng Guodong Xu Huoshun Shi Jianqing Gao Albert Jiarui Li Zhaoyang Wang Lebo Sun Xiajun Li Guofeng Shao Source Type: research

Circular RNA CELF1 drives immunosuppression and anti-PD1 therapy resistance in non-small cell lung cancer via the miR-491-5p/EGFR axis
CONCLUSION: Our data demonstrate that upregulation of circ_CELF1 elicits both oncogenic and immunoregulatory effects on the development of NSCLC. We believe that circ_CELF1 can act as a potential therapeutic target for the treatment of NSCLC.PMID:34788230 | DOI:10.18632/aging.203576 (Source: Aging)
Source: Aging - November 17, 2021 Category: Biomedical Science Authors: Wen Ge Hao Chi Hua Tang Jianjun Xu Jing Wang Wan Cai Haitao Ma Source Type: research

The effect of hyperbaric oxygen therapy on the pathophysiology of skin aging: a prospective clinical trial
Aging (Albany NY). 2021 Nov 16;13(undefined). doi: 10.18632/aging.203701. Online ahead of print.ABSTRACTINTRODUCTION: Skin biopsies can be used to evaluate physiological effects of aging targeted intervention at the tissue/cellular levels. Recent clinical trials have shown that hyperbaric oxygen therapy (HBOT) can target aging hallmarks, including telomere shortening, senescent cells clearance and angiogenesis. The aim of this study was to evaluate the effects of HBOT on the skin of a normal, non-pathological, aging population.METHODS: The study was performed as a prospective clinical trial. After signing informed consent ...
Source: Aging - November 16, 2021 Category: Biomedical Science Authors: Yafit Hachmo Amir Hadanny Sonia Mendelovic Pnina Hillman Eyal Shapira Geva Landau Hadar Gattegno Avi Zrachya Malka Daniel-Kotovsky Merav Catalogna Gregory Fishlev Erez Lang Nir Polak Keren Doenyas Mony Friedman Yonatan Zemel Yair Bechor Shai Efrati Source Type: research

The effect of hyperbaric oxygen therapy on the pathophysiology of skin aging: a prospective clinical trial
Aging (Albany NY). 2021 Nov 16;13(undefined). doi: 10.18632/aging.203701. Online ahead of print.ABSTRACTINTRODUCTION: Skin biopsies can be used to evaluate physiological effects of aging targeted intervention at the tissue/cellular levels. Recent clinical trials have shown that hyperbaric oxygen therapy (HBOT) can target aging hallmarks, including telomere shortening, senescent cells clearance and angiogenesis. The aim of this study was to evaluate the effects of HBOT on the skin of a normal, non-pathological, aging population.METHODS: The study was performed as a prospective clinical trial. After signing informed consent ...
Source: Aging - November 16, 2021 Category: Biomedical Science Authors: Yafit Hachmo Amir Hadanny Sonia Mendelovic Pnina Hillman Eyal Shapira Geva Landau Hadar Gattegno Avi Zrachya Malka Daniel-Kotovsky Merav Catalogna Gregory Fishlev Erez Lang Nir Polak Keren Doenyas Mony Friedman Yonatan Zemel Yair Bechor Shai Efrati Source Type: research

Correction for: Decreased gray matter volume and increased white matter volume in patients with neovascular age-related macular degeneration: a voxel-based morphometry study
Aging (Albany NY). 2021 Nov 14;undefined(undefined). doi: 10.18632/aging.203702. Online ahead of print.NO ABSTRACTPMID:34775373 | DOI:10.18632/aging.203702 (Source: Aging)
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Yan-Kun Shen Qian-Min Ge Yi-Cong Pan Hui-Ye Shu Li-Juan Zhang Qiu-Yu Li Rong-Bin Liang Yi Shao Yao Yu Source Type: research

Identification of KIF4A as a prognostic biomarker for esophageal squamous cell carcinoma
Aging (Albany NY). 2021 Nov 14;13(undefined). doi: 10.18632/aging.203585. Online ahead of print.ABSTRACTEsophageal squamous cell carcinoma (ESCC) is the most common and aggressive tumor worldwide, and the long-term survival of these patients remains poor. Three databases (GSE17351, GSE20347, and GSE100942) were obtained from Gene Expression Omnibus, and 193 differentially expressed genes including 56 upregulated and 137 downregulated genes were identified by paired test using limma R package. Then, functional enrichments by gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed these genes were mainly re...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Lingwei Wang Gang Liu Enkhbat Bolor-Erdene Qinchuan Li Yunqing Mei Lei Zhou Source Type: research

miR-6745-TIMP1 axis inhibits cell growth and metastasis in gastric cancer
In this study, we found that the expression of TIMP1 in GC tissues was upregulated compared with the normal gastric tissues. TIMP1 was confirmed as a direct target of miR-6745 and silencing TIMP1 mimicked the effects of miR-6745 in GC cells. Further mechanism studies have shown that miR-6745 inhibits the Wnt/β-catenin pathway by targeting TIMP1, thereby inhibiting cell proliferation, migration and invasion. In addition, through the analysis of GC tissues, a negative correlation between miR-6745 and TIMP1 was found in 42 GC tissues. Our findings indicate that the miR-6745-TIMP1 axis regulates Wnt/βcatenin signalin...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Hui Liu Yuan Xiang Qi-Bei Zong Xiao-Yu Zhang Zhi-Wen Wang Shi-Qiang Fang Tong-Cun Zhang Xing-Hua Liao Source Type: research

Propofol suppresses the growth and invasion of cervical carcinoma cells by inhibiting MIR155HG
CONCLUSIONS: Altogether, these results indicated that propofol restrained the growth and invasion of cervical cancer cells partly via regulating MIR155HG expression.PMID:34775376 | DOI:10.18632/aging.203697 (Source: Aging)
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Xin-Tan Du Xiao-Yan Wang Ying-He Zheng Da-Peng Liu Source Type: research

Small interfering RNA-induced silencing lncRNA PVT1 inhibits atherosclerosis via inactivating the MAPK/NF- κB pathway
This study investigated the role of lncRNA plasmacytoma variant translocation 1 (PVT1) in AS via the MAPK/NF-κB pathway. Serum samples were collected from AS and non-AS patients. Serum levels of PVT1, CRP, IL-6, IL-1β, and TNF-α were determined. AS mouse model was established and transfected with si-PVT1. Levels of TG, TC, HDL, LDL, MAPK, NF-κB, MMP-2, MMP-9, TIMP-1, and macrophage content were detected. Human arterial vascular smooth muscle cells (HA-VSMCs) induced by 50 mg/mL oxLDL were transfected with si-PVT1 or oe-PVT1 and added with MAPK inhibitor U0126. Viability, apoptosis, cell cycle, colony...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Hong Du Hui Zhang Rong Yang Li Qiao Huiyu Shao Xiaolin Zhang Source Type: research

Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway
Aging (Albany NY). 2021 Nov 11;13(undefined). doi: 10.18632/aging.203684. Online ahead of print.ABSTRACTIn the present study, we found the expression of miR-15a-5p (miR-15a) was increased in glioma tissues, and we further explore the underlying mechanism of miR-15a in glioma progression. Microarray analysis used to identify the differentially expressed microRNAs (miRNAs) in glioma tissues. The expression of miR-15a in glioma tissues and cell lines was tested by qRT-PCR. Luciferase assay was used to determine the binding between miR-15a and Smad7. Wound healing and transwell assay were used to examine the role of miR-15a/Sm...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Yanfeng Guo Xiaopeng Gao Shien An Xin Li Lekun Pan Hongyan Liu Jixiang Liu Jianzhou Gao Zhihuang Zhao Gang Li Yonggang Han Yabin Li Zhisheng Ji Source Type: research

Correction for: Decreased gray matter volume and increased white matter volume in patients with neovascular age-related macular degeneration: a voxel-based morphometry study
Aging (Albany NY). 2021 Nov 14;undefined(undefined). doi: 10.18632/aging.203702. Online ahead of print.NO ABSTRACTPMID:34775373 | DOI:10.18632/aging.203702 (Source: Aging)
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Yan-Kun Shen Qian-Min Ge Yi-Cong Pan Hui-Ye Shu Li-Juan Zhang Qiu-Yu Li Rong-Bin Liang Yi Shao Yao Yu Source Type: research

Identification of KIF4A as a prognostic biomarker for esophageal squamous cell carcinoma
Aging (Albany NY). 2021 Nov 14;13(undefined). doi: 10.18632/aging.203585. Online ahead of print.ABSTRACTEsophageal squamous cell carcinoma (ESCC) is the most common and aggressive tumor worldwide, and the long-term survival of these patients remains poor. Three databases (GSE17351, GSE20347, and GSE100942) were obtained from Gene Expression Omnibus, and 193 differentially expressed genes including 56 upregulated and 137 downregulated genes were identified by paired test using limma R package. Then, functional enrichments by gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed these genes were mainly re...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Lingwei Wang Gang Liu Enkhbat Bolor-Erdene Qinchuan Li Yunqing Mei Lei Zhou Source Type: research

miR-6745-TIMP1 axis inhibits cell growth and metastasis in gastric cancer
In this study, we found that the expression of TIMP1 in GC tissues was upregulated compared with the normal gastric tissues. TIMP1 was confirmed as a direct target of miR-6745 and silencing TIMP1 mimicked the effects of miR-6745 in GC cells. Further mechanism studies have shown that miR-6745 inhibits the Wnt/β-catenin pathway by targeting TIMP1, thereby inhibiting cell proliferation, migration and invasion. In addition, through the analysis of GC tissues, a negative correlation between miR-6745 and TIMP1 was found in 42 GC tissues. Our findings indicate that the miR-6745-TIMP1 axis regulates Wnt/βcatenin signalin...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Hui Liu Yuan Xiang Qi-Bei Zong Xiao-Yu Zhang Zhi-Wen Wang Shi-Qiang Fang Tong-Cun Zhang Xing-Hua Liao Source Type: research

Propofol suppresses the growth and invasion of cervical carcinoma cells by inhibiting MIR155HG
CONCLUSIONS: Altogether, these results indicated that propofol restrained the growth and invasion of cervical cancer cells partly via regulating MIR155HG expression.PMID:34775376 | DOI:10.18632/aging.203697 (Source: Aging)
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Xin-Tan Du Xiao-Yan Wang Ying-He Zheng Da-Peng Liu Source Type: research

Small interfering RNA-induced silencing lncRNA PVT1 inhibits atherosclerosis via inactivating the MAPK/NF- κB pathway
This study investigated the role of lncRNA plasmacytoma variant translocation 1 (PVT1) in AS via the MAPK/NF-κB pathway. Serum samples were collected from AS and non-AS patients. Serum levels of PVT1, CRP, IL-6, IL-1β, and TNF-α were determined. AS mouse model was established and transfected with si-PVT1. Levels of TG, TC, HDL, LDL, MAPK, NF-κB, MMP-2, MMP-9, TIMP-1, and macrophage content were detected. Human arterial vascular smooth muscle cells (HA-VSMCs) induced by 50 mg/mL oxLDL were transfected with si-PVT1 or oe-PVT1 and added with MAPK inhibitor U0126. Viability, apoptosis, cell cycle, colony...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Hong Du Hui Zhang Rong Yang Li Qiao Huiyu Shao Xiaolin Zhang Source Type: research

Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway
Aging (Albany NY). 2021 Nov 11;13(undefined). doi: 10.18632/aging.203684. Online ahead of print.ABSTRACTIn the present study, we found the expression of miR-15a-5p (miR-15a) was increased in glioma tissues, and we further explore the underlying mechanism of miR-15a in glioma progression. Microarray analysis used to identify the differentially expressed microRNAs (miRNAs) in glioma tissues. The expression of miR-15a in glioma tissues and cell lines was tested by qRT-PCR. Luciferase assay was used to determine the binding between miR-15a and Smad7. Wound healing and transwell assay were used to examine the role of miR-15a/Sm...
Source: Aging - November 14, 2021 Category: Biomedical Science Authors: Yanfeng Guo Xiaopeng Gao Shien An Xin Li Lekun Pan Hongyan Liu Jixiang Liu Jianzhou Gao Zhihuang Zhao Gang Li Yonggang Han Yabin Li Zhisheng Ji Source Type: research

Immune cell and TCR/BCR repertoire profiling in systemic lupus erythematosus patients by single-cell sequencing
In this study, we used a single-cell 5' RNA sequence and single-cell T cell receptor (TCR)/B cell receptor (BCR) to study the immune cells and the repertoire from ten SLE patients and the paired normal controls (NC). The results showed that 9732 cells correspondence to 12 cluster immune cell types were identified in NC, whereas 11042 cells correspondence to 16 cluster immune cell types were identified in SLE. The results demonstrated that neutrophil, macrophage, and dendritic cells were accumulated in SLE by annotating the immune cell types. Besides, the bioinformatics analysis of differentially expressed genes (DEGs) in t...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Fengping Zheng Huixuan Xu Cantong Zhang Xiaoping Hong Dongzhou Liu Donge Tang Zuying Xiong Yong Dai Source Type: research

RIP3 Inhibition ameliorates chronic constriction injury-induced neuropathic pain by suppressing JNK signaling
Aging (Albany NY). 2021 Nov 12;13(undefined). doi: 10.18632/aging.203691. Online ahead of print.ABSTRACTNeuroinflammation is a major contributor to neuropathic pain. Receptor interacting serine/threonine kinase 3 (RIP3) senses cellular stress, promotes inflammatory responses and activates c-Jun N-terminal kinase (JNK) signaling. Here, we assessed the involvement of RIP3-induced JNK signaling in chronic constriction injury (CCI)-induced neuropathic pain. We found that RIP3 inhibitors (GSK'872) and JNK inhibitors (SP600125) not only alleviated the radiant heat response and mechanical allodynia in CCI rats, but also reduced i...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Na He Yu-Juan Qu Dan-Yang Li Shou-Wei Yue Source Type: research

Immune cell and TCR/BCR repertoire profiling in systemic lupus erythematosus patients by single-cell sequencing
In this study, we used a single-cell 5' RNA sequence and single-cell T cell receptor (TCR)/B cell receptor (BCR) to study the immune cells and the repertoire from ten SLE patients and the paired normal controls (NC). The results showed that 9732 cells correspondence to 12 cluster immune cell types were identified in NC, whereas 11042 cells correspondence to 16 cluster immune cell types were identified in SLE. The results demonstrated that neutrophil, macrophage, and dendritic cells were accumulated in SLE by annotating the immune cell types. Besides, the bioinformatics analysis of differentially expressed genes (DEGs) in t...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Fengping Zheng Huixuan Xu Cantong Zhang Xiaoping Hong Dongzhou Liu Donge Tang Zuying Xiong Yong Dai Source Type: research

RIP3 Inhibition ameliorates chronic constriction injury-induced neuropathic pain by suppressing JNK signaling
Aging (Albany NY). 2021 Nov 12;13(undefined). doi: 10.18632/aging.203691. Online ahead of print.ABSTRACTNeuroinflammation is a major contributor to neuropathic pain. Receptor interacting serine/threonine kinase 3 (RIP3) senses cellular stress, promotes inflammatory responses and activates c-Jun N-terminal kinase (JNK) signaling. Here, we assessed the involvement of RIP3-induced JNK signaling in chronic constriction injury (CCI)-induced neuropathic pain. We found that RIP3 inhibitors (GSK'872) and JNK inhibitors (SP600125) not only alleviated the radiant heat response and mechanical allodynia in CCI rats, but also reduced i...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Na He Yu-Juan Qu Dan-Yang Li Shou-Wei Yue Source Type: research

Immune cell and TCR/BCR repertoire profiling in systemic lupus erythematosus patients by single-cell sequencing
In this study, we used a single-cell 5' RNA sequence and single-cell T cell receptor (TCR)/B cell receptor (BCR) to study the immune cells and the repertoire from ten SLE patients and the paired normal controls (NC). The results showed that 9732 cells correspondence to 12 cluster immune cell types were identified in NC, whereas 11042 cells correspondence to 16 cluster immune cell types were identified in SLE. The results demonstrated that neutrophil, macrophage, and dendritic cells were accumulated in SLE by annotating the immune cell types. Besides, the bioinformatics analysis of differentially expressed genes (DEGs) in t...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Fengping Zheng Huixuan Xu Cantong Zhang Xiaoping Hong Dongzhou Liu Donge Tang Zuying Xiong Yong Dai Source Type: research

RIP3 Inhibition ameliorates chronic constriction injury-induced neuropathic pain by suppressing JNK signaling
Aging (Albany NY). 2021 Nov 12;13(undefined). doi: 10.18632/aging.203691. Online ahead of print.ABSTRACTNeuroinflammation is a major contributor to neuropathic pain. Receptor interacting serine/threonine kinase 3 (RIP3) senses cellular stress, promotes inflammatory responses and activates c-Jun N-terminal kinase (JNK) signaling. Here, we assessed the involvement of RIP3-induced JNK signaling in chronic constriction injury (CCI)-induced neuropathic pain. We found that RIP3 inhibitors (GSK'872) and JNK inhibitors (SP600125) not only alleviated the radiant heat response and mechanical allodynia in CCI rats, but also reduced i...
Source: Aging - November 13, 2021 Category: Biomedical Science Authors: Na He Yu-Juan Qu Dan-Yang Li Shou-Wei Yue Source Type: research

Low-protein diet applied as part of combination therapy or stand-alone normalizes lifespan and tumor proliferation in a model of intestinal cancer
In conclusion, reduced protein administration can be a very promising form of adjuvant cancer therapy.PMID:34766923 | DOI:10.18632/aging.203692 (Source: Aging)
Source: Aging - November 12, 2021 Category: Biomedical Science Authors: Alina Proske Judith Bossen Jakob von Frieling Thomas Roeder Source Type: research