Identification of prognostic immune-related genes in the tumor microenvironment of endometrial cancer.
Abstract Endometrial cancer (EC) is one of the most common gynecologic malignancies. To identify potential prognostic biomarkers for EC, we analyzed the relationship between the EC tumor microenvironment and gene expression profiles. Using the ESTIMATE R tool, we found that immune and stromal scores correlated with clinical data and the prognosis of EC patients. Based on the immune and stromal scores, 387 intersection differentially expressed genes were identified. Eight immune-related genes were then identified using two machine learning algorithms. Functional enrichment analysis revealed that these genes were ma...
Source: Aging - February 19, 2020 Category: Biomedical Science Authors: Chen P, Yang Y, Zhang Y, Jiang S, Li X, Wan J Tags: Aging (Albany NY) Source Type: research

Cholesterol lowering: to live longer, start younger?
PMID: 32074083 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 19, 2020 Category: Biomedical Science Authors: Wang N, Wilcox I, Lal S Tags: Aging (Albany NY) Source Type: research

Induction of apoptosis and ferroptosis by a tumor suppressing magnetic field through ROS-mediated DNA damage.
In conclusion, this tumor suppressing MF could determine cell fate through ROS-induced DNA damage, inducing oxidative stress and activation of the DNA damage repair pathways, eventually lead to apoptosis and ferroptosis, as well as inhibition of tumor growth. PMID: 32074079 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 18, 2020 Category: Biomedical Science Authors: Yuan LQ, Wang C, Lu DF, Zhao XD, Tan LH, Chen X Tags: Aging (Albany NY) Source Type: research

Exosomes isolated from the plasma of remote ischemic conditioning rats improved cardiac function and angiogenesis after myocardial infarction through targeting Hsp70.
Abstract Remote ischemic conditioning (RIC) is a promising therapeutic strategy to protect heart against ischemic-reperfusion injury. Exosomes have been proved to be an important regulator in many pathological processes. Whether the exosomes derived from RIC could improve cardiac remodeling and function after myocardial infarction (MI) has not been reported. MI animal model was established by ligating the left coronary artery. The bilateral hindlimbs of rats were subjected to RIC treatment using tourniquets. Exosomes were isolated from the plasma of RIC rats and identified by transmission electron microscope. The ...
Source: Aging - February 18, 2020 Category: Biomedical Science Authors: Chen Q, Huang M, Wu J, Jiang Q, Zheng X Tags: Aging (Albany NY) Source Type: research

Metformin: current clinical applications in nondiabetic patients with cancer.
Abstract Metformin is one of the most commonly used first-line oral medications for type 2 diabetes mellitus. Multiple observational studies, reviewed in numerous systematic reviews, have shown that metformin treatment may not only reduce the risk of cancer but may also improve the efficacy of cancer treatment in diabetic patients. Recent studies have been conducted to determine whether a similar protective effect can be demonstrated in nondiabetic cancer patients. However, the results are controversial. The potential optimal dose, schedule, and duration of metformin treatment and the heterogeneity of histological...
Source: Aging - February 18, 2020 Category: Biomedical Science Authors: Chen K, Li Y, Guo Z, Zeng Y, Zhang W, Wang H Tags: Aging (Albany NY) Source Type: research

NMR-based metabonomic analysis of HUVEC cells during replicative senescence.
Abstract Cellular senescence is a physiological process reacting to stimuli, in which cells enter a state of irreversible growth arrest in response to adverse consequences associated with metabolic disorders. Molecular mechanisms underlying the progression of cellular senescence remain unclear. Here, we established a replicative senescence model of human umbilical vein endothelial cells (HUVEC) from passage 3 (P3) to 18 (P18), and performed biochemical characterizations and NMR-based metabolomic analyses. The cellular senescence degree advanced as the cells were sequentially passaged in vitro, and cellular metabol...
Source: Aging - February 17, 2020 Category: Biomedical Science Authors: Yi S, Lin K, Jiang T, Shao W, Huang C, Jiang B, Li Q, Lin D Tags: Aging (Albany NY) Source Type: research

LncRNA FAM83H-AS1 promotes triple-negative breast cancer progression by regulating the miR-136-5p/metadherin axis.
In this study, we evaluated the function and regulation of the long non-coding RNA (lncRNA) FAM83H-AS1 in triple-negative breast cancer (TNBC). Our data show that the FAM83H-AS1 levels are increased in human TNBC cells and tissues. Proliferation, migration, and invasion of TNBC cells are decreased by FAM83H-AS1 suppression, but increased by FAM83H-AS1 overexpression. Bioinformatics analysis revealed that miR-136-5p is a potential target of FAM83H-AS1. MiR-136-5p expression is decreased in TNBC tissues, and its overexpression suppresses TNBC cell proliferation, migration, and invasion. MiR-136-5p suppression reverses the FA...
Source: Aging - February 17, 2020 Category: Biomedical Science Authors: Han C, Fu Y, Zeng N, Yin J, Li Q Tags: Aging (Albany NY) Source Type: research

Cancer stem cell-specific expression profiles reveal emerging bladder cancer biomarkers and identify circRNA_103809 as an important regulator in bladder cancer.
In this study, we describe the differentially-expressed mRNAs, lncRNAs, and circRNAs in BCSCs compared with that in bladder cancer non-stem cells (BCNSCs) through the transcriptome microarray data analysis using bladder cancer patients' specimens. CircRNA_103809, the top one among the highly expressed circRNA identified in BCSCs, promotes the self-renewal, migration and invasion capabilities of bladder cancer by acting as a miR-511 sponge. Additionally, GO and KEGG pathway analysis suggest the differentially expressed genes identified may be involved in the cellular metabolism, differentiation and metastasis regulation of ...
Source: Aging - February 17, 2020 Category: Biomedical Science Authors: Tao T, Yuan S, Liu J, Shi D, Peng M, Li C, Wu S Tags: Aging (Albany NY) Source Type: research

Adiponectin in renal fibrosis.
Abstract Renal fibrosis is an inevitable consequence of parenchymal scarring and is the common final pathway that mediates almost all progressive renal diseases. Adiponectin, a hormone produced by adipose tissue, possesses potent anti-insulin, anti-inflammatory, and anti-fibrotic properties. Reportedly, adiponectin serves as an important messenger that facilitates complex interactions between adipose tissue and other metabolically related organs. In recent years, a growing body of evidence supports adiponectin involvement in renal fibrosis. These studies provide a deeper understanding of the molecular mechanism of...
Source: Aging - February 17, 2020 Category: Biomedical Science Authors: Jing H, Tang S, Lin S, Liao M, Chen H, Fan Y, Zhou J Tags: Aging (Albany NY) Source Type: research

Sarcopenia-related traits and coronary artery disease: a bi-directional Mendelian randomization study.
Abstract Previous Mendelian randomization (MR) studies have yielded a conflicting causal relationship between sarcopenia and coronary artery disease (CAD), and lack the association of CAD with sarcopenia. We performed a bi-directional MR approach to clarify the causality and causal direction between sarcopenia-related traits and CAD. In stage 1 analysis, estimates of inverse variance weighting (IVW) and several sensitivity analyses were obtained by applying genetic variants that predict sarcopenia-related traits to CAD. Conversely, we also applied genetic variants that predict CAD to sarcopenia-related traits in s...
Source: Aging - February 16, 2020 Category: Biomedical Science Authors: Liu HM, Zhang Q, Shen WD, Li BY, Lv WQ, Xiao HM, Deng HW Tags: Aging (Albany NY) Source Type: research

LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke.
In conclusion, it was demonstrated that MEG3 accelerated the process of IS by suppressing miR-424-5p, which targeted Sema3A and the activated MAPK pathway. These results might provide useful information for exploring the potential therapeutic targets in IS. PMID: 32065781 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 16, 2020 Category: Biomedical Science Authors: Xiang Y, Zhang Y, Xia Y, Zhao H, Liu A, Chen Y Tags: Aging (Albany NY) Source Type: research

Ligustilide improves aging-induced memory deficit by regulating mitochondrial related inflammation in SAMP8 mice.
In this study, we used an aging model, SAMP8 mice to investigate the neuroprotective effect of ligustilide. The behavioral tests (Morris water maze, object recognition task, open field test and elevated plus maze) results showed that ligustilide could improve the memory deficit in SAMP8 mice. For mechanism study, we found that the protein level of P-Drp1 (fission) was decreased and the levels of Mfn1 and Mfn2 (fusion) were increased after ligustilide treatment in animals and cells. Ligustilide increased P-AMPK and ATP levels. Malondialdehyde and superoxide dismutase activity results indicated that ligustilide exerts antiox...
Source: Aging - February 16, 2020 Category: Biomedical Science Authors: Zhu WL, Zheng JY, Cai WW, Dai Z, Li BY, Xu TT, Liu HF, Liu XQ, Wei SF, Luo Y, Wang H, Pan HF, Wang Q, Zhang SJ Tags: Aging (Albany NY) Source Type: research

Efficacy of probiotics on cognition, and biomarkers of inflammation and oxidative stress in adults with Alzheimer's disease or mild cognitive impairment - a meta-analysis of randomized controlled trials.
Abstract Probiotics are live microbes that confer health benefits to the host. Preliminary animal evidence supports the potential role of probiotics in ameliorating cognitive health, however, findings from clinical trials in Alzheimer's disease (AD) or mild cognitive impairment (MCI) subjects are controversial. Thus, a meta-analysis is needed to clarify the efficacy of probiotics on cognition in AD or MCI patients. EMBASE, PubMed, Web of Science and Cochrane library were systematically searched and manually screened for relevant published randomized controlled trials (RCTs). Among the 890 citations identified, 5 s...
Source: Aging - February 15, 2020 Category: Biomedical Science Authors: Den H, Dong X, Chen M, Zou Z Tags: Aging (Albany NY) Source Type: research

The IL1 β-HER2-CLDN18/CLDN4 axis mediates lung barrier damage in ARDS.
CONCLUSION: IL-1β promotes the development of ARDS by regulating the IL-1β-HER2/HER3 axis. These findings deepen the understanding of the pathological mechanisms of ARDS. METHODS: Transcription data sets related to ARDS were subjected to differential expression gene analysis, functional enrichment analysis, and receiver operating characteristic curve analysis and, so as to identify core genes in ARDS. Cell experiments were used to further explore the effects of core genes on lung barrier function damage. Animal models were applied to validate the effects of core gene in mediating biological signal pathways i...
Source: Aging - February 15, 2020 Category: Biomedical Science Authors: Ma X, Yu X, Zhou Q Tags: Aging (Albany NY) Source Type: research

Berberine promotes XIAP-mediated cells apoptosis by upregulation of miR-24-3p in acute lymphoblastic leukemia.
CONCLUSIONS: miR-24-3p/PIM-2/XIAP signaling contributes to BBR-mediated leukemia mitigation in p53-defect ALL, which should be further developed as a treatment strategy in ALL patients with p53 deficiency. METHODS: Cell viability and apoptosis were determined using CCK-8 and TUNEL assays, respectively. The dual-luciferase reporter gene system was used to determine the interaction between miR-24-3p and 3'-untranslated regions (UTRs) of PIM-2. PMID: 32062612 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 13, 2020 Category: Biomedical Science Authors: Liu J, Chen Z, Cui Y, Wei H, Zhu Z, Mao F, Wang Y, Liu Y Tags: Aging (Albany NY) Source Type: research

LINC00623/miR-101/HRAS axis modulates IL-1 β-mediated ECM degradation, apoptosis and senescence of osteoarthritis chondrocytes.
LINC00623/miR-101/HRAS axis modulates IL-1β-mediated ECM degradation, apoptosis and senescence of osteoarthritis chondrocytes. Aging (Albany NY). 2020 Feb 12;12: Authors: Lü G, Li L, Wang B, Kuang L Abstract Chondrocyte apoptosis and extracellular matrix (ECM) degeneration have been implicated in the pathogenesis of osteoarthritis (OA). Based on previously reported microarray analysis, HRAS (Harvey rat sarcoma viral oncogene homolog), a member of the RAS protein family, was chosen as a potential regulator of OA chondrocyte apoptosis and ECM degradation. HRAS expression was downregulated in O...
Source: Aging - February 12, 2020 Category: Biomedical Science Authors: Lü G, Li L, Wang B, Kuang L Tags: Aging (Albany NY) Source Type: research

Orai, STIM, and PMCA contribute to reduced calcium signal generation in CD8+ T cells of elderly mice.
, Lis A Abstract Ca2+ is a crucial second messenger for proper T cell function. Considering the relevance of Ca2+ signals for T cell functionality it is surprising that no mechanistic insights into T cell Ca2+ signals from elderly individuals are reported. The main Ca2+ entry mechanism in T cells are STIM-activated Orai channels. Their role during lymphocyte aging is completely unknown. Here, we report not only reduced Ca2+ signals in untouched and stimulated, but also in central and effector memory CD8+ T cells from elderly (18-24 months) compared to adult (3-6 months) mice. Two mechanisms contribute to the overa...
Source: Aging - February 12, 2020 Category: Biomedical Science Authors: Angenendt A, Steiner R, Knörck A, Schwär G, Konrad M, Krause E, Lis A Tags: Aging (Albany NY) Source Type: research

A nomogram combining long non-coding RNA expression profiles and clinical factors predicts survival in patients with bladder cancer.
Abstract Bladder cancer (BCa) is a heterogeneous disease with various tumorigenic mechanisms and clinical behaviors. The current tumor-node-metastasis (TNM) staging system is inadequate to predict overall survival (OS) in BCa patients. We developed a BCa-specific, long-non-coding-RNA (lncRNA)-based nomogram to improve survival prediction in BCa. We obtained the large-scale gene expression profiles of samples from 414 BCa patients in The Cancer Genome Atlas database. Using an lncRNA-mining computational framework, we identified three OS-related lncRNAs among 826 lncRNAs that were differentially expressed between BC...
Source: Aging - February 12, 2020 Category: Biomedical Science Authors: Wang Y, Du L, Yang X, Li J, Li P, Zhao Y, Duan W, Chen Y, Wang Y, Mao H, Wang C Tags: Aging (Albany NY) Source Type: research

Circular RNA hsa_circ_0056836 functions an oncogenic gene in hepatocellular carcinoma through modulating miR-766-3p/FOSL2 axis.
CONCLUSION: In summary, the hsa_circ_0056836 / miR-766-3p / FOSL2 axis may serve as a promising strategy for HCC treatment. METHOD: First, the expressions of hsa_circ_0056836 in HCC tissues and corresponding para-cancerous tissues as well as in HCC cell lines and normal hepatocytes THLE-3 were detected by RT-PCR. Subcellular localization of hsa_circ_0056836 was confirmed by FISH. To detect the association between hsa_circ_0056836 and miR-766-3p, AGO2-RIP and Luciferase reporter assay were adopted. Loss of function study was applied to assess the role of hsa_circ_0056836 in HCC and to determine tumorigenesis in nude mi...
Source: Aging - February 12, 2020 Category: Biomedical Science Authors: Li Z, Liu Y, Yan J, Zeng Q, Hu Y, Wang H, Li H, Li J, Yu Z Tags: Aging (Albany NY) Source Type: research

Adaptive selection in the evolution of programmed cell death-1 and its ligands in vertebrates.
In this study, we aligned protein-coding genes from vertebrate species to evaluate positive selection constraints and evolution in the PD1, PD-L1 and PD-L2 genes conserved across up to 166 vertebrate species, with an average of 55 species per gene. We determined that although the positive selection was obvious, an average of 5.3% of codons underwent positive selection in the three genes across vertebrate lineages, and increased positive selection pressure was detected in both the Ig-like domains and transmembrane domains of the proteins. Moreover, the PD1, PD-L1 and PD-L2 genes were highly expressed in almost all tissues o...
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Ahmad HI, Zhou J, Ahmad MJ, Afzal G, Jiang H, Zhang X, Elokil AA, Khan MA, Li L, Li H, Ping L, Chen J Tags: Aging (Albany NY) Source Type: research

Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis.
In this study, we aimed to identify a candidate drug that can activate endogenous Angiopoietin 1 (Ang1) expression via drug repositioning as a pharmacological treatment for avascular osteonecrosis. After incubation with 821 drugs from the Food and Drug Administration (FDA)-approved drug library, Ang1 expression in U2OS cell culture media was examined by ELISA. Metformin, the first-line medication for treatment of type 2 diabetes, was selected as a candidate for in vitro and in vivo experimental evaluation. Ang1 was induced, and alkaline phosphatase activity was increased by metformin treatment in U2OS and MG63 cells. Wound...
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Park SH, Kang MA, Moon YJ, Jang KY, Kim JR Tags: Aging (Albany NY) Source Type: research

Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma.
CONCLUSIONS: Overall, these findings uncover a role for OLA1 in regulating the proliferation and apoptosis of HCC cells. MATERIALS AND METHODS: The Cancer Genome Atlas and Gene Expression Omnibus datasets were analyzed to identify gene expression. Immunohistochemistry staining, western blot and real-time polymerase chain reaction were performed to evaluate OLA1 expression in samples. Cell count Kit-8, wound-healing, transwell and flow cytometry assays were used to analyze HCC cell progression. Subcutaneous xenotransplantation models were used to investigate the role of OLA1 in vivo. Coimmunoprecipitation was used to a...
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Huang S, Zhang C, Sun C, Hou Y, Zhang Y, Tam NL, Wang Z, Yu J, Huang B, Zhuang H, Zhou Z, Ma Z, Sun Z, He X, Zhou Q, Hou B, Wu L Tags: Aging (Albany NY) Source Type: research

Functional lncRNA-miRNA-mRNA networks in rabbit carotid atherosclerosis.
In this study, we induced atherosclerotic plaques in three rabbit carotid arteries through an atherogenic diet and balloon injury; three age-matched rabbits were fed normal chow and served as controls. We thoroughly investigated the RNA (mRNA, lncRNA and miRNA) expression profiles in atherosclerotic rabbit carotid models with deep RNA sequencing. We identified several significantly differentially expressed RNAs. The corresponding lncRNA-miRNA-mRNA network was constructed, and the significantly dysregulated network was selected. Furthermore, Gene Ontology and  Kyoto Encyclopedia of Genes and Genomes analyses indicated ...
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Wu Y, Zhang F, Lu R, Feng Y, Li X, Zhang S, Hou W, Tian J, Kong X, Sun L Tags: Aging (Albany NY) Source Type: research

Immune-related key gene CLDN10 correlates with lymph node metastasis but predicts favorable prognosis in papillary thyroid carcinoma.
In conclusion, although CLDN1 might be correlated with LNM, it may also increase the infiltration of immune cells, including CD8+T cells and macrophages, and improve the clinical outcomes of patients with PTC. The effects of tumor purity and immune cell infiltration need to be considered in prognosis evaluation. PMID: 32045884 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Xiang Z, Zhong C, Chang A, Ling J, Zhao H, Zhou W, Zhuo X Tags: Aging (Albany NY) Source Type: research

Cerebral small vessel disease is associated with gait disturbance among community-dwelling elderly individuals: the Taizhou imaging study.
Abstract Gait disturbance is considered to be a significant clinical manifestation of cerebral small vessel disease (CSVD). We aimed to investigate the association between different imaging markers of CSVD or total CSVD burden and gait disturbance in a community-dwelling population. In the cross-sectional Taizhou Imaging Study (TIS), 314 participants free of neurological disorders underwent MRI scanning and gait assessment with quantitative wearable devices as well as clinical rating scales. In linear regression, after adjustment for demographics and vascular risks, total CSVD burden was associated with prolonged ...
Source: Aging - February 11, 2020 Category: Biomedical Science Authors: Li P, Wang Y, Jiang Y, Zhang K, Yang Q, Yuan Z, Zhu Z, Tang W, Fan M, Ye W, Dong Q, Jin L, Ding D, Cui M, Chen X Tags: Aging (Albany NY) Source Type: research

Single-cell RNA sequencing of immune cells in gastric cancer patients.
In this study, we characterized the heterogeneity of T cells isolated from GC patients at the single-cell level using single-cell RNA sequencing. We identified different immune cell subtypes and their heterogeneous transcription factors and depicted their developmental trajectories. In particular, we focused on exhausted CD8+ cells and Tregs and discovered that, as compared to control, the IRF8 transcription factor was downregulated in CD8+ tumour-infiltrating lymphocytes (TILs) from GC tissues, and that GC patients with lower IRF8 levels in blood CD8+ T cells tended to be a at a more advanced disease stage. These findings...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Fu K, Hui B, Wang Q, Lu C, Shi W, Zhang Z, Rong D, Zhang B, Tian Z, Tang W, Cao H, Wang X, Chen Z Tags: Aging (Albany NY) Source Type: research

Resilience, disease and the age of single cell science.
PMID: 32039835 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Simons M, Chen PY, Chittenden TW Tags: Aging (Albany NY) Source Type: research

LDL-C plays a causal role on T2DM: a Mendelian randomization analysis.
Abstract Diabetic dyslipidemia is a common condition in patients with Type 2 diabetes mellitus (T2DM). However, with the increasing application of statins which mainly decrease low-density lipoprotein cholesterol (LDL-C) levels, clinical trials and meta-analysis showed a clearly increase of the incidence of new-onset DMs, partly due to genetic factors. To determine whether a causal relationship exists between LDL-C and T2DM, we conducted a two-sample Mendelian Randomization (MR) analysis using genetic variations as instrumental variables (IVs). Initially, 29 SNPs significantly related to LDL-C (P≤ 5.0×10-...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Pan W, Sun W, Yang S, Zhuang H, Jiang H, Ju H, Wang D, Han Y Tags: Aging (Albany NY) Source Type: research

Age-specific differential changes on gut microbiota composition in patients with major depressive disorder.
This study was performed to identify the age-specific differential gut microbiota in MDD patients. In total, 70 MDD patients and 71 healthy controls (HCs) were recruited and divided into two groups: young group (age 18-29 years) and middle-aged group (age 30-59 years). The 16S rRNA gene sequences were extracted from the collected fecal samples. Finally, we found that the relative abundances of Firmicutes and Bacteroidetes were significantly decreased and increased, respectively, in young MDD patients as compared with young HCs, and the relative abundances of Bacteroidetes and Actinobacteria were significantly decreased and...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Chen JJ, He S, Fang L, Wang B, Bai SJ, Xie J, Zhou CJ, Wang W, Xie P Tags: Aging (Albany NY) Source Type: research

Screening and identification of genes associated with cell proliferation in cholangiocarcinoma.
Abstract Cholangiocarcinoma (CCA), an aggressive tumor with poor prognosis, is a malignant cancer with increasing incidence and mortality rates. It is important to survey crucial genes in CCA to find and design potential drug targets, especially for those genes associated with cell proliferation that is a key biological process in tumorgenesis. Herein, we surveyed genes associated with cell proliferation via a comprehensive pan-cancer analysis. Candidate genes were further analyzed using multiple approaches, including cross-analysis from diverse molecular levels, examination of potential function and interactions,...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Guo L, Zhang Y, Yin Z, Ji Y, Yang G, Qian B, Li S, Wang J, Liang T, Li C, Li X Tags: Aging (Albany NY) Source Type: research

EGF released from human placental mesenchymal stem cells improves premature ovarian insufficiency via NRF2/HO-1 activation.
Abstract Human placental mesenchymal stem cells (hPMSCs) have the ability to release cytokines and to differentiate into the three germ layers. To date, the relevance of hPMSCs for the treatment of premature ovarian insufficiency (POI) disease through the regulation of oxidative stress is still unclear. Therefore, to evaluate the therapeutic efficiency and investigate the mechanism of hPMSCs, we generated a mouse model of POI and collected human ovarian granule cells (hGCs) from patients with POI. hPMSCs displayed therapeutic effects on POI ovarian function, including recovered follicular numbers and increased exp...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Ding C, Zou Q, Wu Y, Lu J, Qian C, Li H, Huang B Tags: Aging (Albany NY) Source Type: research

Association of serum retinoic acid with depression in patients with acute ischemic stroke.
This study was aimed to evaluate the relationship between RA levels in serum and post-stroke depression (PSD). A single-center (Chengdu, China) prospective cohort study was conducted on patients with acute ischemic stroke. The RA serum level was measured at admission. The PSD was assessed in the 3-month follow-up. The RA-PSD relationship was evaluated with conditional logistic regression. In total, 239 ischemic stroke cases and 100 healthy controls were included. The median RA serum level in patients with ischemic stroke was 2.45 ng/ml (interquartile range [IQR], 0.72-4.33), lower(P
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Yang CD, Cheng ML, Liu W, Zeng DH Tags: Aging (Albany NY) Source Type: research

microRNA-1203 targets and silences cyclophilin D to protect human endometrial cells from oxygen and glucose deprivation-re-oxygenation.
Abstract Oxygen and glucose deprivation (OGD)-re-oxygenation (OGDR) stimulation to the human endometrial cells mimics ischemia-reperfusion injury. Cyclophilin D (CypD)-dependent programmed necrosis pathway mediates OGDR-induced cytotoxicity to human endometrial cells. We here identified a novel CypD-targeting miRNA, microRNA-1203 (miR-1203). In T-HESC and primary human endometrial cells, ectopic overexpression of miR-1203, using a lentiviral construct, potently downregulated the CypD 3'-untranslated region (3'-UTR) activity and its expression. Both were however upregulated in endometrial cells with forced miR-1203...
Source: Aging - February 10, 2020 Category: Biomedical Science Authors: Xu HB, Zheng YF, Wu D, Li Y, Zhou LN, Chen YG Tags: Aging (Albany NY) Source Type: research

Profiles of immune cell infiltration and immune-related genes in the tumor microenvironment of osteosarcoma.
Abstract This work aimed to investigate tumor-infiltrating immune cells (TIICs) and immune-associated genes in the tumor microenvironment of osteosarcoma. An algorithm known as ESTIMATE was applied for immune score assessment, and osteosarcoma cases were assigned to the high and low immune score groups. Immune-associated genes between these groups were compared, and an optimal immune-related risk model was built by Cox regression analyses. The deconvolution algorithm (referred to as CIBERSORT) was applied to assess 22 TIICs for their amounts in the osteosarcoma microenvironment. Osteosarcoma cases with high immune...
Source: Aging - February 9, 2020 Category: Biomedical Science Authors: Zhang C, Zheng JH, Lin ZH, Lv HY, Ye ZM, Chen YP, Zhang XY Tags: Aging (Albany NY) Source Type: research

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis.
Abstract The lncRNA tumor suppressor candidate 8 (TUSC8) plays a critical role in the development of several cancers. However, the biological functions and underlying molecular mechanisms of TUSC8 with respect to breast cancer remain largely unclear. Here, we found that TUSC8 was significantly down-regulated in breast cancer tissues and its high expression predicted better prognosis of breast cancer patients. Functionally, knock-down of TUSC8 drastically promoted the proliferation, migration and invasion of breast cancer cells in vitro and facilitated tumorigenicity and metastasis in vivo. Mechanistically, the res...
Source: Aging - February 9, 2020 Category: Biomedical Science Authors: Zhao L, Zhou Y, Zhao Y, Li Q, Zhou J, Mao Y Tags: Aging (Albany NY) Source Type: research

Choline as a prevention for Alzheimer's disease.
PMID: 32039834 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 9, 2020 Category: Biomedical Science Authors: Velazquez R, Winslow W, Mifflin MA Tags: Aging (Albany NY) Source Type: research

Tfh cell subset biomarkers and inflammatory markers are associated with frailty status and frailty subtypes in the community-dwelling older population: a cross-sectional study.
Abstract We conducted a cross-sectional study investigating community-dwelling older population to determine association between immunoscenescence marker, inflammatory cytokines and frailty. Frailty status was classified with 33-item modified frailty index and latent class analysis was applied to explore the latent classes (subtypes) of frailty. In multivariable analysis, higher Tfh2 cells were associated with a higher risk of frailty [1.13(1.03-1.25)] in females, but a lower risk of cognitive and functional frail [0.92(0.86-0.99)] and physiological frail [0.92(0.87-0.98)]. Additionally, a greater risk of multi-fr...
Source: Aging - February 8, 2020 Category: Biomedical Science Authors: Yin MJ, Xiong YZ, Xu XJ, Huang LF, Zhang Y, Wang XJ, Xiu LC, Huang JX, Lian TY, Liang DM, Zen JM, Ni JD Tags: Aging (Albany NY) Source Type: research

Riluzole: a therapeutic strategy in Alzheimer's disease by targeting the WNT/ β-catenin pathway.
Riluzole: a therapeutic strategy in Alzheimer's disease by targeting the WNT/β-catenin pathway. Aging (Albany NY). 2020 Feb 08;12: Authors: Vallée A, Vallée JN, Guillevin R, Lecarpentier Y Abstract Alzheimer's disease (AD) is a neurodegenerative disease, where the etiology remains unclear. AD is characterized by amyloid-(Aβ) protein aggregation and neurofibrillary plaques deposits. Oxidative stress and chronic inflammation have been suggested as causes of AD. Glutamatergic pathway dysregulation is also mainly associated with AD process. In AD, the canonical WNT/β-catenin p...
Source: Aging - February 8, 2020 Category: Biomedical Science Authors: Vallée A, Vallée JN, Guillevin R, Lecarpentier Y Tags: Aging (Albany NY) Source Type: research

Global burden of larynx cancer, 1990-2017: estimates from the global burden of disease 2017 study.
This study aimed to investigate the health burden of larynx cancer at global, regional, and national levels. We collected data of larynx cancer between 1990 and 2017 from the Global Burden of Disease study, including incidence, mortality, and disability adjusted life-years (DALYs). Estimated annual percentage changes (EAPCs) were calculated to assess the changes in age-standardized rate (ASR) of larynx cancer. From 1990 to 2017, LC incident cases increased by 58.67%; however, age-standardized incidence rate (ASIR) decreased, with an EAPC of -0.99. Additionally, the incident cases and ASIR of LC were 6-fold higher for male ...
Source: Aging - February 8, 2020 Category: Biomedical Science Authors: Deng Y, Wang M, Zhou L, Zheng Y, Li N, Tian T, Zhai Z, Yang S, Hao Q, Wu Y, Song D, Zhang D, Lyu J, Dai Z Tags: Aging (Albany NY) Source Type: research

Cell-to-cell lactate shuttle operates in heart and is important in age-related heart failure.
Abstract Recent studies have revealed a resemblance of a HIF-regulated heart and brain glycolytic profiles prompting the hypothesis that the classical cell-to-cell lactate shuttle observed between astrocytes and neurons operates also in heart - between cardiac fibroblasts and cardiomyocytes. Here, we demonstrate that co-culturing of cardiomyocytes with cardiac fibroblasts leads to orchestrated changes in expression and/or localization pattern of glucose metabolism enzymes and lactate transport proteins in both cell types. These changes are regulated by paracrine signaling using microvesicle-packed and soluble fact...
Source: Aging - February 8, 2020 Category: Biomedical Science Authors: Gizak A, McCubrey JA, Rakus D Tags: Aging (Albany NY) Source Type: research

Atorvastatin reduces cerebral vasospasm and infarction after aneurysmal subarachnoid hemorrhage in elderly Chinese adults.
Abstract We explored whether acute atorvastatin treatment would improve clinical outcomes and reduce the incidence of cerebral vasospasm after aneurysmal subarachnoid hemorrhage in elderly Chinese adults. Patients (60 to 90 years old) were admitted to intensive care units after surgery to clip or embolize their aneurysms. We assessed 592 patients and assigned 159 to receive atorvastatin (20 mg/day) and 158 to receive placebo once daily for up to 14 days. The primary outcome was the Glasgow outcome scale at 6 months, and secondary outcomes were cerebral vasospasm, 30-days all-cause mortality, cerebral infarction, a...
Source: Aging - February 7, 2020 Category: Biomedical Science Authors: Chen J, Li M, Zhu X, Chen L, Yang S, Zhang C, Wu T, Feng X, Wang Y, Chen Q Tags: Aging (Albany NY) Source Type: research

Identifying lncRNA-miRNA-mRNA networks to investigate Alzheimer's disease pathogenesis and therapy strategy.
This study presents the first systematic dissection of lncRNA-associated ceRNA profiles in the APP/PS1 mouse brain. The identified lncRNA-associated ceRNA networks could provide insights that facilitate AD diagnosis and future treatment strategies. PMID: 32035423 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 7, 2020 Category: Biomedical Science Authors: Ma N, Tie C, Yu B, Zhang W, Wan J Tags: Aging (Albany NY) Source Type: research

The nuclear localization signal-mediated nuclear targeting of herpes simplex virus 1 early protein UL2 is important for efficient viral production.
Abstract Herpes simplex virus 1 (HSV-1) is a representative alphaherpesvirus that can provoke a series of severe diseases to human being, but its exact pathogenesis is not perfectly understood. UL2, a uracil-DNA glycosylase involved in the process of HSV-1 DNA replication, has been shown to be predominantly targeted to the nuclei in our previous study, yet little is established regarding the subcellular localization signal or its related function of UL2 during HSV-1 propagation. Here, by creating a number of UL2 variants merged with enhanced yellow fluorescent protein, an authentic nuclear localization signal (NLS...
Source: Aging - February 7, 2020 Category: Biomedical Science Authors: Li M, Zou X, Wang Y, Xu Z, Ou X, Li Y, Liu D, Guo Y, Deng Y, Jiang S, Li T, Shi S, Bao Y, Peng T, Cai M Tags: Aging (Albany NY) Source Type: research

Meta-analytic evidence for the anti-aging effect of hormesis on Caenorhabditis elegans.
Abstract Mild stress-induced hormesis, as a promising strategy to improve longevity and healthy aging, meets both praise and criticism. To comprehensively assess the applicability of hormesis in aging intervention, this meta-analysis was conducted focusing on the effect of hormesis on Caenorhabditis elegans. Twenty-six papers involving 198 effect size estimates met the inclusion criteria. Meta-analytic results indicated that hormesis could significantly extend the mean lifespan of C. elegans by 16.7% and 25.1% under normal and stress culture conditions (p
Source: Aging - February 7, 2020 Category: Biomedical Science Authors: Sun T, Wu H, Cong M, Zhan J, Li F Tags: Aging (Albany NY) Source Type: research

Bone morphogenetic protein 9, and its genetic variants contribute to susceptibility of idiopathic pulmonary arterial hypertension.
Abstract Considering the predominant role of rare variants of the Bone morphogenetic protein 9 (BMP9) gene in the occurrence of idiopathic pulmonary arterial hypertension (IPAH), here we conducted a case-control study, together with functional validation, to explore the relationships between variants of the BMP9 gene and development of IPAH. We found minor alleles of rs3740297 (OR: 0.72, 95% CI: 0.59-0.87, P=7.77×10-5) and rs7923671 (OR: 0.76, 95% CI: 0.62-0.93, P=0.009) were significantly associated with decreased risk of IPAH. Minor alleles of rs3740297 and rs7923671 were significantly associated with incr...
Source: Aging - February 7, 2020 Category: Biomedical Science Authors: Guo K, Xu L, Jin L, Wang H, Ren Y, Hu Y, Yu J, Cang J Tags: Aging (Albany NY) Source Type: research

Targeting aged bone marrow for systemic rejuvenation.
PMID: 32028261 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - February 6, 2020 Category: Biomedical Science Authors: Alibhai FJ, Li RK Tags: Aging (Albany NY) Source Type: research

Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer.
Abstract Notch refers to a set of genes encoding a family of transmembrane receptors in mammalian cells. Previous evidence indicated that Notch receptors were implicated in the onset of gastric cancer. However, there is little evidence on the different genetic expression patterns of the four Notch receptors and their values for patient prognosis. Most recently, we investigated the transcriptional data of Notch receptors and related patient survival in patients with GC based on several databases, including ONCOMINE, GEPIA, Kaplan-Meier Plotter, cBioPortal and TIMER. According to our findings, gastric cancer tissues...
Source: Aging - February 6, 2020 Category: Biomedical Science Authors: Hu J, Yu J, Gan J, Song N, Shi L, Liu J, Zhang Z, Du J Tags: Aging (Albany NY) Source Type: research

Methionine increases yolk production to offset the negative effect of caloric restriction on reproduction without affecting longevity in C. elegans.
In this study, by using several CR strategies in C. elegans, we examine key functions of reproduction including embryonic development and larvae growth. We find that CR significantly decreases the survival of embryos and slows the growth of the offspring. We further determine that defect in oocyte but not sperm is responsible for the compromised reproduction under CR. Interestingly, adding methionine to the medium reverses the reproduction defects, but does not affect the long lifespan resulted from CR. The beneficial effect of methionine on reproduction requires the yolk protein vitellogenin. CR down-regulates vitellogeni...
Source: Aging - February 6, 2020 Category: Biomedical Science Authors: Zhou G, Huang C, Xing L, Li L, Jiang Y, Wei Y Tags: Aging (Albany NY) Source Type: research

Identification of an immune-related risk signature for predicting prognosis in clear cell renal cell carcinoma.
In this study, we identified an immune-related, five-gene signature that improves survival prediction in ccRCC. Patients were classified as high- and low-risk based on the signature risk score. Survival analysis showed differential prognosis, while principal component analysis revealed distinctly different immune phenotypes between the two risk groups. High-risk patients tended to have advanced stage, higher grade disease, and poorer prognoses. Functional enrichment analysis showed that the signature genes were mainly involved in the cytokine-cytokine receptor interaction pathway. Moreover, we found that tumors from high-r...
Source: Aging - February 6, 2020 Category: Biomedical Science Authors: Hua X, Chen J, Su Y, Liang C Tags: Aging (Albany NY) Source Type: research

A high neutrophil-to-lymphocyte ratio predicts hemorrhagic transformation of large atherosclerotic infarction in patients with acute ischemic stroke.
Abstract Increasing evidence suggests that inflammation is associated with the development of acute ischemic stroke (AIS). The neutrophil-to-lymphocyte ratio (N/L) is an important marker of inflammation and is highly correlated with mortality in stroke patients in recent studies. The N/L of patients who experience hemorrhagic transformation (HT) after AIS is know, but any relationship between N/L and large artery atherosclerosis (LAA) remains unclear, this is our present topic. We enrolled 185 patients with LAA-type HT in the development cohort from a prospective, consecutive, hospital-based stroke registry to thi...
Source: Aging - February 6, 2020 Category: Biomedical Science Authors: Zhang WB, Zeng YY, Wang F, Cheng L, Tang WJ, Wang XQ Tags: Aging (Albany NY) Source Type: research