Platelet-rich aggregates in MNC collection circuit
Transfusion. 2021 Mar 4. doi: 10.1111/trf.16288. Online ahead of print.NO ABSTRACTPMID:33660852 | DOI:10.1111/trf.16288 (Source: Transfusion)
Source: Transfusion - March 4, 2021 Category: Hematology Authors: Nisar Amin Joseph H Cho Phillip McMullen Imran Uraizee Geoffrey Wool Leon Su Source Type: research

A pilot study of the effect of rivaroxaban in sickle cell anemia
CONCLUSIONS: Rivaroxaban was safe but did not significantly decrease coagulation activation, endothelial activation, or inflammation. Rivaroxaban did not improve microvascular blood flow. Adequately powered studies are required to further evaluate the efficacy of rivaroxaban in SCD. Clinicaltrials.gov Identifier: NCT02072668.PMID:33660875 | DOI:10.1111/trf.16343 (Source: Transfusion)
Source: Transfusion - March 4, 2021 Category: Hematology Authors: Kenneth I Ataga Laila Elsherif David Wichlan Adane F Wogu Neil Matsui Rafal Pawlinski Jianwen Cai Nigel S Key Source Type: research

Targeting the neonatal Fc receptor (FcRn) to treat autoimmune diseases and maternal-fetal immune cytopenias
Transfusion. 2021 Mar 2. doi: 10.1111/trf.16341. Online ahead of print.ABSTRACTFcRn, a non-classical Fc gamma (γ) receptor (FcγR) with near ubiquitous expression, plays key roles in disease pathogenesis and progression though immunoglobulin G (IgG) transport, IgG recycling, and IgG-immune complex clearance. FcRn function can be inhibited using IgG-based and non-IgG-based antagonists, by exploiting the pH-dependent binding affinity of FcRn for the IgG Fc region. FcRn therapeutics have shown promise in murine models and human clinical trials for autoimmune diseases and maternal-fetal immune cytopenias; they appea...
Source: Transfusion - March 2, 2021 Category: Hematology Authors: Sarah L Wyckoff Krystalyn E Hudson Source Type: research

The impact of health-related quality of life and depressive symptoms on blood donor career-Results from the Danish blood donor study
CONCLUSION: MCS, PCS, and MDI score affect donor career. Thus, adjusting for donation frequency may reduce HDE-bias in donor health research. However, because of the small effect sizes, other ways of quantifying HDE may be beneficial.PMID:33650703 | DOI:10.1111/trf.16336 (Source: Transfusion)
Source: Transfusion - March 2, 2021 Category: Hematology Authors: Maria Didriksen Lise W Th ørner Margit A H Larsen Erik S ørensen Kristoffer Burgdorf Susan Mikkelsen Klaus Rostgaard Karina Banasik Ole B Pedersen Christian Erikstrup Kaspar R Nielsen Mie T Bruun Henrik Hjalgrim Henrik Ullum Source Type: research

Targeting the neonatal Fc receptor (FcRn) to treat autoimmune diseases and maternal-fetal immune cytopenias
Transfusion. 2021 Mar 2. doi: 10.1111/trf.16341. Online ahead of print.ABSTRACTFcRn, a non-classical Fc gamma (γ) receptor (FcγR) with near ubiquitous expression, plays key roles in disease pathogenesis and progression though immunoglobulin G (IgG) transport, IgG recycling, and IgG-immune complex clearance. FcRn function can be inhibited using IgG-based and non-IgG-based antagonists, by exploiting the pH-dependent binding affinity of FcRn for the IgG Fc region. FcRn therapeutics have shown promise in murine models and human clinical trials for autoimmune diseases and maternal-fetal immune cytopenias; they appea...
Source: Transfusion - March 2, 2021 Category: Hematology Authors: Sarah L Wyckoff Krystalyn E Hudson Source Type: research

The impact of health-related quality of life and depressive symptoms on blood donor career-Results from the Danish blood donor study
CONCLUSION: MCS, PCS, and MDI score affect donor career. Thus, adjusting for donation frequency may reduce HDE-bias in donor health research. However, because of the small effect sizes, other ways of quantifying HDE may be beneficial.PMID:33650703 | DOI:10.1111/trf.16336 (Source: Transfusion)
Source: Transfusion - March 2, 2021 Category: Hematology Authors: Maria Didriksen Lise W Th ørner Margit A H Larsen Erik S ørensen Kristoffer Burgdorf Susan Mikkelsen Klaus Rostgaard Karina Banasik Ole B Pedersen Christian Erikstrup Kaspar R Nielsen Mie T Bruun Henrik Hjalgrim Henrik Ullum Source Type: research

National level adjustments to the challenges of the SARS-CoV2 pandemic on blood banking operations
CONCLUSION: Trends and reaction patterns of blood banking reported by our study may be useful in future planning and adjustments of blood banking activities.PMID:33644858 | DOI:10.1111/trf.16346 (Source: Transfusion)
Source: Transfusion - March 1, 2021 Category: Hematology Authors: Andrea Matusovits S ándor Nagy Kl ára Baróti-Tóth J ános Nacsa M ária Lázár Imelda Marton Hajnalka Andrikovics Zoltan Voko Attila Tordai Source Type: research

National level adjustments to the challenges of the SARS-CoV2 pandemic on blood banking operations
CONCLUSION: Trends and reaction patterns of blood banking reported by our study may be useful in future planning and adjustments of blood banking activities.PMID:33644858 | DOI:10.1111/trf.16346 (Source: Transfusion)
Source: Transfusion - March 1, 2021 Category: Hematology Authors: Andrea Matusovits S ándor Nagy Kl ára Baróti-Tóth J ános Nacsa M ária Lázár Imelda Marton Hajnalka Andrikovics Zoltan Voko Attila Tordai Source Type: research

Blood type change identifies late dominance reversal of chimerism after double umbilical cord blood transplantation with review of the literature
Transfusion. 2021 Feb 26. doi: 10.1111/trf.16297. Online ahead of print.ABSTRACTBACKGROUND: A 30-year-old man underwent double umbilical cord blood transplantation (UCBT) for acute myeloid leukemia (AML) with reduced intensity conditioning. The cords had identical HLA types and were each a 5/6 match to the patient. Following transplantation, cord 2 initially dominated all tested cell populations. At day +306, we observed an unusual reversal of dominance chimerism pattern in which cord 1 instead dominated all tested populations.STUDY DESIGN & METHODS: Polymerase chain reaction (PCR)-based short tandem repeat (STR) assay...
Source: Transfusion - February 27, 2021 Category: Hematology Authors: Andrea P Ho Bethany E Ho Mary P Berg Jonathan A Gutman Nicole L Draper Source Type: research

Blood type change identifies late dominance reversal of chimerism after double umbilical cord blood transplantation with review of the literature
Transfusion. 2021 Feb 26. doi: 10.1111/trf.16297. Online ahead of print.ABSTRACTBACKGROUND: A 30-year-old man underwent double umbilical cord blood transplantation (UCBT) for acute myeloid leukemia (AML) with reduced intensity conditioning. The cords had identical HLA types and were each a 5/6 match to the patient. Following transplantation, cord 2 initially dominated all tested cell populations. At day +306, we observed an unusual reversal of dominance chimerism pattern in which cord 1 instead dominated all tested populations.STUDY DESIGN & METHODS: Polymerase chain reaction (PCR)-based short tandem repeat (STR) assay...
Source: Transfusion - February 27, 2021 Category: Hematology Authors: Andrea P Ho Bethany E Ho Mary P Berg Jonathan A Gutman Nicole L Draper Source Type: research

A novel modified thaw-siphon method for extracting cryoprecipitate: The Bokutoh-siphon method
CONCLUSION: A new in-house CRYO preparation method was established by modifying a previously used thaw-siphon method. A coagulation factor-rich CRYO was extracted from plasma frozen at -40°C along with the first fraction of thawed plasma, without using a large-capacity refrigerated centrifuge for blood bags.PMID:33634868 | DOI:10.1111/trf.16331 (Source: Transfusion)
Source: Transfusion - February 26, 2021 Category: Hematology Authors: Shigeko Nishimura Miyuki Teratani Tomoko Igarashi Michiyo Nakahara Yuki Hazama Sae Ohtani Hiroshi Fujita Source Type: research

New blood donors in times of crisis: Increased donation willingness, particularly among people at high risk for attracting SARS-CoV-2
In this report, we examined how the pandemic affected new donor registrations in the Netherlands, especially among groups with higher risk profiles for severe COVID-19. Additionally, we explored the role of media for blood donation and new donor registrations.STUDY DESIGN AND METHODS: We analyzed new donor registrations and attention for blood donation in newspapers and on social media from January until May 2020, in comparison to the same period in 2017 to 2019.RESULTS: After the introduction of nationwide prevention measures, several peaks in new donor registrations occurred, which coincided with peaks in media attention...
Source: Transfusion - February 26, 2021 Category: Hematology Authors: Marloes L C Spekman Steven Ramondt Franke A Quee Femmeke J Prinsze Elisabeth M J Huis In 't Veld Katja van den Hurk Eva-Maria Merz Source Type: research

A monthly roundup of key articles in other journals
Transfusion. 2021 Feb 25. doi: 10.1111/trf.16325. Online ahead of print.NO ABSTRACTPMID:33629354 | DOI:10.1111/trf.16325 (Source: Transfusion)
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Caitlin McOmish Source Type: research

Releasates of riboflavin/UV-treated platelets: Microvesicles suppress cytokine-mediated endothelial cell migration/proliferation
CONCLUSION: This study identified an inhibitory impact of PMVs on endothelial cell migration/proliferation upon stimulation by released cytokines and PMVs from PLTs treated with RF/UV light for endothelial cell wound closure.PMID:33629371 | DOI:10.1111/trf.16337 (Source: Transfusion)
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Peter Schubert Luka Culibrk Brankica Culibrk Edward M Conway Raymond P Goodrich Dana V Devine Source Type: research

Survey of newborn direct antiglobulin testing practice in United States and Canadian transfusion services
CONCLUSION: There is variability in newborn immunohematology testing and transfusion practice and potential overutilization of the DAT. Evidence-based consensus guidelines should be developed to standardize practice and to improve safety.PMID:33629748 | DOI:10.1111/trf.16335 (Source: Transfusion)
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Elizabeth P Crowe Ruchika Goel Jennifer Andrews Erin K Meyer Trisha E Wong Steven R Sloan Meghan Delaney Lani Lieberman Melissa M Cushing Source Type: research

Impact of body weight on hemoglobin increments in adult red blood cell transfusion
Transfusion. 2021 Feb 25. doi: 10.1111/trf.16338. Online ahead of print.ABSTRACTBACKGROUND: Though weight is a major consideration when transfusing blood in pediatric patients, it is generally not considered when dosing transfusions in adults. We hypothesized that the change in hemoglobin (Hb) concentration is inversely proportional to body weight when transfusing red blood cells (RBC) in adults.METHODS: A total of 13,620 adult surgical patients at our institution were assessed in this retrospective cohort study (2009-2016). Patients were stratified based on total body weight (kg): 40-59.9 (16.6%), 60-79.9 (40.4%), 80-99.9...
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Brian D Lo Brian C Cho Nadia B Hensley Nicolas C Cruz Eric A Gehrie Steven M Frank Source Type: research

A monthly roundup of key articles in other journals
Transfusion. 2021 Feb 25. doi: 10.1111/trf.16325. Online ahead of print.NO ABSTRACTPMID:33629354 | DOI:10.1111/trf.16325 (Source: Transfusion)
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Caitlin McOmish Source Type: research

Releasates of riboflavin/UV-treated platelets: Microvesicles suppress cytokine-mediated endothelial cell migration/proliferation
CONCLUSION: This study identified an inhibitory impact of PMVs on endothelial cell migration/proliferation upon stimulation by released cytokines and PMVs from PLTs treated with RF/UV light for endothelial cell wound closure.PMID:33629371 | DOI:10.1111/trf.16337 (Source: Transfusion)
Source: Transfusion - February 25, 2021 Category: Hematology Authors: Peter Schubert Luka Culibrk Brankica Culibrk Edward M Conway Raymond P Goodrich Dana V Devine Source Type: research

Could antigen loss be a potential mechanism to explain antibody-mediated immune suppression?
Transfusion. 2021 Feb 24. doi: 10.1111/trf.16309. Online ahead of print.NO ABSTRACTPMID:33624837 | DOI:10.1111/trf.16309 (Source: Transfusion)
Source: Transfusion - February 24, 2021 Category: Hematology Authors: Yoelys Cruz-Leal Alan H Lazarus Source Type: research

Apheresis Physician Well-Being During the COVID-19 Pandemic: Results of a Survey
CONCLUSION: There is a widespread decline in AP well-being during the COVID-19 pandemic that was not adequately improved by institutional or personal resources/actions taken. Institutions and physicians must work together to implement strategies including resources and actions that could further improve AP physician well-being during a public health crisis.PMID:33619750 | DOI:10.1111/trf.16340 (Source: Transfusion)
Source: Transfusion - February 23, 2021 Category: Hematology Authors: Yvette C Tanhehco Yanhua Li Nicole D Zantek Joanne Becker Mohamed Alsammak Kael Mikesell Ding Wen Wu Tisha Foster Vishesh Chhibber Marisa Saint Martin Gay Wehrli Source Type: research

Apheresis Physician Well-Being During the COVID-19 Pandemic: Results of a Survey
CONCLUSION: There is a widespread decline in AP well-being during the COVID-19 pandemic that was not adequately improved by institutional or personal resources/actions taken. Institutions and physicians must work together to implement strategies including resources and actions that could further improve AP physician well-being during a public health crisis.PMID:33619750 | DOI:10.1111/trf.16340 (Source: Transfusion)
Source: Transfusion - February 23, 2021 Category: Hematology Authors: Yvette C Tanhehco Yanhua Li Nicole D Zantek Joanne Becker Mohamed Alsammak Kael Mikesell Ding Wen Wu Tisha Foster Vishesh Chhibber Marisa Saint Martin Gay Wehrli Source Type: research

Identification of a novel B allele with a nucleotide deletion (c.103_106 delG) in the ABO gene associated with a B weak phenotype
Transfusion. 2021 Feb 22. doi: 10.1111/trf.16302. Online ahead of print.NO ABSTRACTPMID:33615482 | DOI:10.1111/trf.16302 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Yunlei He Lu Yu Deyi Xu Jiwei Zhang Gang Deng Source Type: research

Longitudinal analysis of SARS-CoV-2 antibodies in 8000 U.S. first-time convalescent plasma donations
Transfusion. 2021 Feb 22. doi: 10.1111/trf.16291. Online ahead of print.ABSTRACTBACKGROUND: Coronavirus disease 2019 (COVID-19) convalescent individuals carry antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that, through a plasma donation, can be used as a potential therapeutic either in direct transfusion or for the manufacture of hyperimmune globulin (HIG). The success of such interventions depends on the antibody potency in such plasma donations, but little information on the collection of potent units is currently available.STUDY DESIGN AND METHODS: A total of 8749 plasma units, collecte...
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Michael Karbiener Maria R Farcet Reinhard Ilk Jessica Schreiner James Lenart Nicholas Powers Joseph M Stewart Hema Tallman Thomas R Kreil Source Type: research

Retrieval transfusion protocol in New South Wales, Australia: A retrospective review of the first 5 years
This article describes the trends and characteristics of New South Wales Ambulance RTP activations.MATERIALS AND METHODS: This retrospective database review examines the patient records for all RTP activations. Key areas of investigation include logistics, product requests, population demographics, etiologies, physiology, mission timings, and transfusions.RESULTS: Ambulance medical teams attended 27 531 missions in the reviewed period, 1573 patients received transfusion, and there were 138 RTP activations. Blood products were sourced from 40 banks and transported by police (46.7%), ambulance (27.1%), and helicopter (13.0%)...
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Sophie Shand Kate Curtis Michael Dinh Brian Burns Source Type: research

Impact of primary culture: A true positive culture rate mystery
Transfusion. 2020 Oct;60(10):2459-2460. doi: 10.1111/trf.16023.NO ABSTRACTPMID:33615496 | DOI:10.1111/trf.16023 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Stephen J Wagner Pampee P Young Source Type: research

Mind the evidence gap: The need for trials in the prevention and management of perioperative anemia in noncardiac surgery
Transfusion. 2020 Oct;60(10):2168-2170. doi: 10.1111/trf.16103.NO ABSTRACTPMID:33615497 | DOI:10.1111/trf.16103 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Dean A Fergusson Manoj M Lalu Source Type: research

Leukocytapheresis for AML hyperleukocytosis: Failing to make the grade
Transfusion. 2020 Oct;60(10):2161-2163. doi: 10.1111/trf.16091.NO ABSTRACTPMID:33615498 | DOI:10.1111/trf.16091 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Laura Connelly-Smith Michael L Linenberger Source Type: research

Response to Walker et al.: Residual bacterial detection rates after primary culture as determined by secondary culture and rapid testing
Transfusion. 2020 Oct;60(10):2460-2461. doi: 10.1111/trf.16072.NO ABSTRACTPMID:33615499 | DOI:10.1111/trf.16072 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Paul D Mintz Remo P Vallejo Source Type: research

Improved accuracy in counting residual white blood cells in red cell concentrates using new blood bank mode software of SYSMEX XN-1000 hematology analyzer
Transfusion. 2020 Oct;60(10):2456-2457. doi: 10.1111/trf.15985.NO ABSTRACTPMID:33615500 | DOI:10.1111/trf.15985 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Johan W Lagerberg Dirk de Korte Source Type: research

Thrombocytopenia and platelet transfusion in myelodysplastic syndromes
Transfusion. 2020 Oct;60(10):2164-2167. doi: 10.1111/trf.16093.NO ABSTRACTPMID:33615501 | DOI:10.1111/trf.16093 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: David Bowen Marina Karakantza Source Type: research

Educating donors about deferrals through the provision of tailored materials: Findings from a survey of deferred donors
CONCLUSIONS: The application of a deferral is often misunderstood by donors. Providing educational materials to donors can increase their understanding and may lead to increased retention of donors through rebooking of subsequent donations.PMID:33615506 | DOI:10.1111/trf.16212 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Carley N Gemelli Sarah P Kruse Amanda Thijsen Nina Van Dyke Tanya E Davison Source Type: research

How red blood cell quality is starting to carry its weight
Transfusion. 2021 Feb;61(2):336-339. doi: 10.1111/trf.16264.NO ABSTRACTPMID:33616923 | DOI:10.1111/trf.16264 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Kevin E Shopsowitz Andrew W Shih Source Type: research

Fatal sepsis associated with a storage container leak permitting platelet contamination with environmental bacteria after pathogen reduction
CONCLUSION: Findings are compatible with post-processing environmental contamination of a pathogen reduced platelet concentrate via a non-visible, acquired storage container leak. Efforts are warranted to actively prevent damage to, and detect defects in, platelet storage containers, and to store and transport components in clean environments.PMID:33616945 | DOI:10.1111/trf.16210 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Emmanuel A Fadeyi Stephen J Wagner Corinne Goldberg Thea Lu Pampee Young Peter W Bringmann Nathaniel M Meier Andrew M Namen Richard J Benjamin Elizabeth Palavecino Source Type: research

Donor screening question alternatives to men who have sex with men time deferral: Potential impact on donor deferral and discomfort
CONCLUSION: Most donors are comfortable answering alternative questions (except very personal ones) but question the benefit. Implementing alternative questions would result in substantive deferrals. Other policies such as using an MSM capture question to ask additional questions only to MSM should be considered.PMID:33616949 | DOI:10.1111/trf.16165 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Sheila F O'Brien Mindy Goldman Pierre Robillard Lori Osmond Genevi ève Myhal Élise Roy Source Type: research

What is clinically significant bleeding?
Transfusion. 2021 Feb;61(2):340-343. doi: 10.1111/trf.16277.NO ABSTRACTPMID:33616956 | DOI:10.1111/trf.16277 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Khai Li Chai Erica M Wood Source Type: research

CME Title Page
Transfusion. 2021 Jan;61(1):255. doi: 10.1111/trf.16222.NO ABSTRACTPMID:33616958 | DOI:10.1111/trf.16222 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Source Type: research

Potentially clinically significant anti-Di(b) identified by monocyte monolayer assay before transfusion
Transfusion. 2021 Jan;61(1):331-332. doi: 10.1111/trf.16114.NO ABSTRACTPMID:33616959 | DOI:10.1111/trf.16114 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Donald R Branch Mairead B Holton Tammy Ison Jacqueline Cote Catherine Dube Wendy Lau Source Type: research

Time to stop worrying about ABO incompatible cryoprecipitate transfusions in adults
Transfusion. 2021 Jan;61(1):1-4. doi: 10.1111/trf.16228.NO ABSTRACTPMID:33616962 | DOI:10.1111/trf.16228 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Jenna Khan Nancy M Dunbar Source Type: research

Decrease in serum antibodies to SARS-CoV-2 in convalescent plasma donors over time
Transfusion. 2021 Feb;61(2):651-654. doi: 10.1111/trf.16172.NO ABSTRACTPMID:33616966 | DOI:10.1111/trf.16172 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Kristina Prus Caroline R Alquist Jose A Cancelas David Oh Source Type: research

Retrospective analysis of outcomes in patients with acute hypertriglyceridemic pancreatitis treated without therapeutic plasma exchange
CONCLUSIONS: HTGP patients who do not receive TPE do not experience inferior outcomes compared to patients in the literature treated with TPE. The added value of TPE in HTGP, if any exists, needs to be demonstrated in controlled trials.PMID:33616967 | DOI:10.1111/trf.16214 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Bailey Hutchison Julia Collins Robert S Makar Walter Dzik Source Type: research

Testing for the presence of SARS-CoV-2 RNA in presymptomatic blood donors
Transfusion. 2021 Feb;61(2):649-651. doi: 10.1111/trf.16155.NO ABSTRACTPMID:33616976 | DOI:10.1111/trf.16155 (Source: Transfusion)
Source: Transfusion - February 22, 2021 Category: Hematology Authors: Lionel Loubaki Pierre Gantner Am élie Pagliuzza Hugues Fausther-Bovendo Gary Kobinger Nicolas Chomont Marc Germain Source Type: research

A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections
Transfusion. 2021 Feb 19. doi: 10.1111/trf.16323. Online ahead of print.ABSTRACTINTRODUCTION: Little is known about the neutralizing (nAb) and binding antibody kinetics in COVID-19 convalescent plasma donors, especially during the first 100 days after disease onset.MATERIALS AND METHODS: A cohort of previously RT-PCR positive (detected by nasopharyngeal swab during the acute phase), male convalescent patients, all with mild symptoms, were enrolled in serial blood sample collection for a longitudinal nAb titers and anti-nucleocapsid (NP) antibodies (IgM, IgG and IgA) evaluation. NAbs were detected by a cytopathic effect-bas...
Source: Transfusion - February 19, 2021 Category: Hematology Authors: Silvano Wendel Rita Font ão-Wendel Roberta Fachini Gabriela Candelaria Patricia Scuracchio Ruth Achkar Mayra Brito Luiz Fernando Reis Anamaria Camargo Mariane Amano Rafael Machado Danielle Araujo Camila Soares Edison Durigon Source Type: research

High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
CONCLUSION: We found that the level of antibodies targeting the full-length SARS-CoV-2 Spike declines gradually after the resolution of the infection. This decline was not related to the number of donations but strongly correlated with the decline of RBD-specific antibodies and the number of days post-symptom onset. These findings help to better understand the decline of humoral responses against the SARS-CoV-2 Spike and provide important information on when to collect plasma after recovery from active infection for convalescent plasma transfusion.PMID:33604922 | DOI:10.1111/trf.16318 (Source: Transfusion)
Source: Transfusion - February 19, 2021 Category: Hematology Authors: Sai Priya Anand J érémie Prévost Jonathan Richard Jos ée Perreault Tony Tremblay Mathieu Drouin Marie-Jos ée Fournier Antoine Lewin Ren ée Bazin Andr és Finzi Source Type: research

Hepatitis E virus: Efficacy of pasteurization of plasma-derived VWF/FVIII concentrate determined by pig bioassay
CONCLUSION: This study demonstrates that pasteurization at 60°C for 10 h of an HEV-positive plasma derivative leads to the effective reduction of infectivity, resulting in a VWF/FVIII product with an appropriate margin of safety for HEV.PMID:33605455 | DOI:10.1111/trf.16298 (Source: Transfusion)
Source: Transfusion - February 19, 2021 Category: Hematology Authors: Lisa D ähnert Josephine Schlosser Christine Fast Andreas Fr öhlich Albrecht Gr öner Elke Lange Nathan J Roth Wolfram Sch äfer Charlotte Schr öder Martin Eiden Martin H Groschup Source Type: research

A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections.
gon E Abstract INTRODUCTION: Little is known about the neutralizing (nAb) and binding antibody kinetics in COVID-19 convalescent plasma donors, especially during the first 100 days after disease onset. MATERIALS AND METHODS: A cohort of previously RT-PCR positive (detected by nasopharyngeal swab during the acute phase), male convalescent patients, all with mild symptoms, were enrolled in serial blood sample collection for a longitudinal nAb titers and anti-nucleocapsid (NP) antibodies (IgM, IgG and IgA) evaluation. NAbs were detected by a cytopathic effect-based virus neutralization test (CPE-based VNT...
Source: Transfusion - February 19, 2021 Category: Hematology Authors: Wendel S, Fontão-Wendel R, Fachini R, Candelaria G, Scuracchio P, Achkar R, Brito M, Reis LF, Camargo A, Amano M, Machado R, Araujo D, Soares C, Durigon E Tags: Transfusion Source Type: research

Hepatitis E virus: Efficacy of pasteurization of plasma-derived VWF/FVIII concentrate determined by pig bioassay.
CONCLUSION: This study demonstrates that pasteurization at 60°C for 10 h of an HEV-positive plasma derivative leads to the effective reduction of infectivity, resulting in a VWF/FVIII product with an appropriate margin of safety for HEV. PMID: 33605455 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - February 19, 2021 Category: Hematology Authors: Dähnert L, Schlosser J, Fast C, Fröhlich A, Gröner A, Lange E, Roth NJ, Schäfer W, Schröder C, Eiden M, Groschup MH Tags: Transfusion Source Type: research

High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples.
CONCLUSION: We found that the level of antibodies targeting the full-length SARS-CoV-2 Spike declines gradually after the resolution of the infection. This decline was not related to the number of donations but strongly correlated with the decline of RBD-specific antibodies and the number of days post-symptom onset. These findings help to better understand the decline of humoral responses against the SARS-CoV-2 Spike and provide important information on when to collect plasma after recovery from active infection for convalescent plasma transfusion. PMID: 33604922 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - February 18, 2021 Category: Hematology Authors: Anand SP, Prévost J, Richard J, Perreault J, Tremblay T, Drouin M, Fournier MJ, Lewin A, Bazin R, Finzi A Tags: Transfusion Source Type: research

Post-expiry stability of freeze-dried plasma under field conditions - Can shelf life be extended?
Abstract BACKGROUND: This prospective study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the safety and efficacy of Lyo-Plas N Freeze-Dried Plasma (FDP) at the end of the manufacturer's shelf life, and up to 24 months post expiry. Clotting factors V, VIII and XI, proteins S, C, fibrinogen, PTT, ATIII, VWF, and INR as well as TEG, DDM, residual moisture, pH, and sterility of FDP returned from field units after uncontrolled storage were evaluated. STUDY DESIGN AND METHODS: Parameters measured at the end of manufacturer shelf life, as well as 6, 12, 18, and 24 ...
Source: Transfusion - February 17, 2021 Category: Hematology Authors: Zur M, Gorenbein P, Nachshon A, Radomislensky I, Tsur AM, Benov A, Wagnert-Avraham L, Glassberg E Tags: Transfusion Source Type: research

Factors driving availability of COVID-19 convalescent plasma: Insights from a demand, production, and supply model.
CONCLUSIONS: Epidemic trajectory was the most important determinant of ability to meet demand for CCP, although our simulations revealed several contributing operational drivers of CCP program success. PMID: 33590906 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - February 16, 2021 Category: Hematology Authors: Russell WA, Grebe E, Custer B Tags: Transfusion Source Type: research

HIV transmission network analysis allows identifying unreported risk factors in HIV-positive blood donors in France.
CONCLUSION: Network inference can be used to complement declaration data on risk factors for HIV infection in BDs. This approach, complementary to behavioral studies, is a valuable tool to evaluate the effect of changes in deferral criteria on BD compliance. PMID: 33592129 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - February 16, 2021 Category: Hematology Authors: Cappy P, Chaillon A, Pillonel J, Essat A, Chaix ML, Meyer L, Barin F, Tiberghien P, Laperche S Tags: Transfusion Source Type: research