Antioxidant prevents clearance of hemostatically competent platelets after long-term cold storage.
CONCLUSIONS: Long-term cold storage induces mitochondrial uncoupling and increased proton leak and ROS generation. The resulting ROS is a crucial contributor to the increased macrophage-dependent and -independent clearance of functional PLTs and can be prevented by the antioxidant NAC in a magnesium-containing additive solution. PMID: 33247486 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 27, 2020 Category: Hematology Authors: Hegde S, Wellendorf AM, Zheng Y, Cancelas JA Tags: Transfusion Source Type: research

Altering the landscape of transfusion safety.
PMID: 33245559 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 27, 2020 Category: Hematology Authors: Kaufman R Tags: Transfusion Source Type: research

Mobilization characteristics, blood graft composition, and outcome in diffuse large B-cell lymphoma after autologous stem cell transplantation: Results from the prospective multicenter GOA study.
CONCLUSION: The mobilization capacity of CD34+ cells impacted on early hematologic recovery in patients with DLBCL after auto-SCT. Higher graft CD34+ cell count and both CD34+ CD133+ CD38- and CD3+ cells were also associated with better OS. The effect of optimal graft cellular composition on outcome in DLBCL should be evaluated in a randomized study. PMID: 33245582 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 27, 2020 Category: Hematology Authors: Partanen A, Turunen A, Valtola J, Pyörälä M, Vasala K, Kuittinen O, Kuitunen H, Penttilä K, Keskinen L, Kuittinen T, Mäntymaa P, Pelkonen J, Varmavuo V, Jantunen E Tags: Transfusion Source Type: research

HLA molecule expression on the surface of cells and microparticles in platelet concentrates.
CONCLUSION: Platelets or residual cells remaining after leukoreduction are not the only source of HLA Class I molecules in PCs, highlighting the contribution of MPs to alloimmunization mechanisms. These data may be relevant for the development of new transfusion guidelines. PMID: 33241556 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 25, 2020 Category: Hematology Authors: Pannetier L, Tamagne M, Bocquet T, Pirenne F, Ansart-Pirenne H, Vingert B Tags: Transfusion Source Type: research

Two new RHD alleles with deletions spanning multiple exons.
CONCLUSIONS: Resolution of unclear or inconclusive results from targeted genotyping arrays often leads to the discovery of new alleles. The 5' Rhesus box may be a hot spot for genetic recombination events, such as the large deletions described in this report. PMID: 33241598 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 25, 2020 Category: Hematology Authors: Matteocci A, Monge-Ruiz J, Stef M, Apraiz I, Herrera-Del-Val L, Mancuso T, Fennell K, Lopez M, Larizgoitia-Martin Y, Nespoli G, Rubia-Tejero M, Collaretti A, Pierelli L, Ochoa-Garay G Tags: Transfusion Source Type: research

Higher donor body mass index is associated with increased hemolysis of red blood cells at 42-days of storage: A retrospective analysis of routine quality control data.
Abstract BACKGROUND: For reasons unclear, some stored red blood cells (RBCs) have low hemolysis, while others have high hemolysis, which impacts quality consistency. To identify variables that influence hemolysis, routine quality control (QC) data for 42-days-stored RBCs with corresponding donor information were analyzed. STUDY DESIGN AND METHODS: RBC QC and donor data were obtained from a national blood supplier. Regression models and analyses were performed on total cohort stratified by donor sex and by high hemolysis (≥90th percentile) vs control (
Source: Transfusion - November 24, 2020 Category: Hematology Authors: Sparrow RL, Payne KA, Adams GG Tags: Transfusion Source Type: research

Diversity of variant alleles encoding Kidd, Duffy, and Kell antigens in individuals with sickle cell disease using whole genome sequencing data from the NHLBI TOPMed Program.
CONCLUSIONS: We described the diversity and distribution of SLC14A1, ACKR1, and KEL variants in two large SCD cohorts, comprising mostly individuals of mixed ancestry. This information may be useful for planning the transfusion support of patients with SCD. PMID: 33231305 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 24, 2020 Category: Hematology Authors: Dinardo CL, Oliveira TGM, Kelly S, Ashley-Koch A, Telen M, Schmidt LC, Castilho S, Melo K, Dezan MR, Wheeler MM, Johnsen JM, Nickerson DA, Jain D, Custer B, Pereira AC, Sabino EC, NHLBI Recipient Epidemiology Donor Evaluation Study (REDS-III) Internationa Tags: Transfusion Source Type: research

The effect of gamma irradiation on platelet redox state during storage.
CONCLUSION: Gamma irradiation can significantly increase cytosolic ROS generation in two distinct phases, one upon irradiation and another later in longer-stored PCs. While earlier ROS influx seems to be governed by direct effect of irradiation, the second phase of oxidant stress is presumably due to the storage-dependent PLT activation. Intriguingly, these observations were also in line with early P-selectin increments and increased PS exposure in longer-stored PLTs. Given the mutual link between ROS generation and PLT activation, further investigation is required to explore the effect of gamma irradiation on the inductio...
Source: Transfusion - November 24, 2020 Category: Hematology Authors: Nodeh FK, Hosseini E, Ghasemzadeh M Tags: Transfusion Source Type: research

Therapeutic Plasma Exchange for COVID-19-Associated Hyperviscosity.
CONCLUSIONS: This series demonstrates the utility of TPE to rapidly correct increased blood viscosity in patients with COVID-19-associated hyperviscosity. Large randomized trials are needed to determine whether TPE may improve clinical outcomes for patients with COVID-19. PMID: 33231313 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 24, 2020 Category: Hematology Authors: Truong AD, Auld SC, Barker NA, Friend S, Wynn AT, Cobb J, Sniecinski RM, Tanksley CL, Polly DM, Gaddh M, Connor M, Nakahara H, Sullivan HC, Kempton C, Guarner J, Duncan A, Josephson CD, Roback JD, Stowell SR, Maier CL Tags: Transfusion Source Type: research

A retrospective study assessing the characteristics of COVID-19 convalescent plasma donors and donations.
CONCLUSION: This study demonstrates the feasibility and safety of a pilot CP program conducted in Italy. The identification of factors (i.e., age and severity of COVID-19) positively associated with higher neutralizing antibody titers at the time of donation may help to optimize the selection of CP donors. PMID: 33231325 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 24, 2020 Category: Hematology Authors: Del Fante C, Franchini M, Baldanti F, Percivalle E, Glingani C, Marano G, Mengoli C, Mortellaro C, Viarengo G, Perotti C, Liumbruno GM Tags: Transfusion Source Type: research

Missed irradiation of cellular blood components for vulnerable patients: Insights from 10  years of SHOT data.
CONCLUSION: Leucodepletion appears to reduce the risk for TA-GvHD. None of 12 cases of TA-GvHD reported to SHOT prior to introduction of LD occurred in patients with conditions recommended for irradiated components by current guidelines. Irradiation indefinitely for all stages of HL is not based on good evidence and is a difficult guideline to follow. Further research on long-term immune function in HL is required. Variation between different national guidelines reflects the very limited evidence. PMID: 33219533 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 21, 2020 Category: Hematology Authors: Elliot J, Narayan S, Poles D, Tuckley V, Bolton-Maggs PHB Tags: Transfusion Source Type: research

Evaluation of A plasma for incompatible patients.
CONCLUSION: No evidence of ABO immune-mediated hemolysis was observed in the patient population. The results of the study support the safety of A plasma transfusion in B and AB patients. We hypothesize the relationship observed between A plasma volume and mortality/ICU stay may be from collinearity with disease severity. PMID: 33219552 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 21, 2020 Category: Hematology Authors: Olsen G, Passwater M, Huggins M, Mukhtar F, Pelletier P Tags: Transfusion Source Type: research

Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.
CONCLUSIONS: Combining photosensitizers in pathogen reduction methods could improve bactericidal efficacy and lead to use of lower concentrations of photosensitizers to reduce toxicities and unwanted side effects. PMID: 33219568 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 21, 2020 Category: Hematology Authors: Feyissa Q, Xu F, Ibrahim Z, Li Y, Xu KL, Guo Z, Ahmad J, Vostal JG Tags: Transfusion Source Type: research

A monthly roundup of key articles in other journals.
PMID: 33215700 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 20, 2020 Category: Hematology Authors: McOmish C Tags: Transfusion Source Type: research

Blood component utilization in COVID-19 patients in New York City: Transfusions do not follow the curve.
CONCLUSION: There is a need to closely monitor the blood product inventory and demand throughout the COVID-19 pandemic as patients' transfusion needs may increase over time. Daily or weekly trending of patients' clinical status and laboratory values may assist blood banks in inventory management. PMID: 33215718 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 20, 2020 Category: Hematology Authors: DeSimone RA, Costa VA, Kane K, Sepulveda JL, Ellsworth GB, Gulick RM, Zucker J, Sobieszcyk ME, Schwartz J, Cushing MM Tags: Transfusion Source Type: research

Maintaining adequate donations and a sustainable blood supply: Lessons learned.
CONCLUSIONS: Collecting and maintaining a blood supply robust enough to handle disasters and transfusion-dependent patients in need of specialty products is challenging. Collaboration of all parties should help to optimize resources, ensure appropriate collections, improve patient care, and ultimately result in a robust, sustainable blood supply. PMID: 33206404 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 18, 2020 Category: Hematology Authors: Gammon RR, Rosenbaum L, Cooke R, Friedman M, Rockwood L, Nichols T, Vossoughi S Tags: Transfusion Source Type: research

A novel variant DO*A allele with a c.370delT mutation leading to a DO-null phenotype in a Syrian family.
PMID: 33206405 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 18, 2020 Category: Hematology Authors: Morin PA, Ethier C, Lavoie J, Robitaille N, Baillargeon N Tags: Transfusion Source Type: research

Residual red cells in blood components: A multisite study of fully automated enumeration using a hematology analyzer.
CONCLUSION: The BB mode had acceptable performance characteristics and has the potential for integration into a fully automated process control system for rRBC enumeration in plasma and PCs. PMID: 33202065 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 17, 2020 Category: Hematology Authors: Cavagnetto C, Alejo Blanco R, McKenna H, Willmott L, Aydogdu E, Akinyemi N, Standring H, Procter S, Lagerberg JW, Johansson E, Croxon H, de Korte D, Garner SF, Shirakami A, Saker J, Linssen J, Cardigan R Tags: Transfusion Source Type: research

Lack of association between SNPsrs8176719(O blood group) and COVID-19: data from Spanish age matched patients and controls.
mez J PMID: 33191530 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 16, 2020 Category: Hematology Authors: Coto E, Albaiceta GM, Clemente MG, Gómez J Tags: Transfusion Source Type: research

A novel c.82insC (p.Tyr28Leufs85X) mutation in the XK gene associated with the McLeod phenotype.
PMID: 33190237 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 15, 2020 Category: Hematology Authors: Horikawa T, Tanaka M, Kamada I, Yoshise Y, Okuda K, Tateyama H, Otani S, Takihara Y Tags: Transfusion Source Type: research

A novel DO*01 silent allele associated with a nucleotide insertion in a Brazilian patient with anti-Gya.
PMID: 33190238 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 15, 2020 Category: Hematology Authors: Bub CB, Aravechia MG, Dos Santos LD, Cruz KVD, Duarte K, Garcia P, Vendrame TAP, Arnoni C, Kutner JM, Castilho L Tags: Transfusion Source Type: research

Frequent platelet donation is associated with lymphopenia and risk of infections: A nationwide cohort study.
CONCLUSION: Frequent plateletpheresis donation using an LRS chamber was associated with CD4+ T-cell lymphopenia and an increased risk of infections. These findings suggest a need to monitor T-lymphocyte counts in frequent platelet donors and to conduct future investigations of long-term donor health and for regulators to consider steps to mitigate lymphodepletion in donors. PMID: 33186486 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 13, 2020 Category: Hematology Authors: Zhao J, Gabriel E, Norda R, Höglund P, Baden L, Diedrich BA, Marits P, Enoksson SL, Gansner JM, Kaufman R, Dickman PW, Edgren G Tags: Transfusion Source Type: research

Identification of a novel B allele with a c.917  T >  C mutation on the ABO*B.01 allele.
Identification of a novel B allele with a c.917 T> C mutation on the ABO*B.01 allele. Transfusion. 2020 Nov 12;: Authors: Liang S, Su YQ, Liang YL, Wu F, Peng L, Xu YP PMID: 33179775 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 12, 2020 Category: Hematology Authors: Liang S, Su YQ, Liang YL, Wu F, Peng L, Xu YP Tags: Transfusion Source Type: research

Long-term return and donation pattern of those who begin donating at different ages: A retrospective cohort analysis of blood donors in Australia.
CONCLUSIONS: To continuously ensure the short- to mid-term sufficiency of blood supply in Australia, targeted recruitment of donors aged 30-years and above may be considered, however its feasibility and impact should be explored further given relatively smaller proportion of new donors are middle-aged and older under current policies. Future studies with a longer follow-up period are needed to examine whether the frequency of donation among those who start donating at a younger age increases later in their life when they are 30-years or over. PMID: 33179790 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 12, 2020 Category: Hematology Authors: Priyono A, Masser BM, Dyda A, Davison TE, Irving DO, Karki S Tags: Transfusion Source Type: research

Reduced sensitivity of PLASMIC and French scores for the diagnosis of thrombotic thrombocytopenic purpura in older individuals.
CONCLUSION: The PLASMIC and French TTP scores have reduced sensitivity at age ≥ 60 years and are less reliable in identifying TTP in older patients. A high index of suspicion and availability of rapid ADAMTS13 assays is required to correctly diagnose all patients with TTP. PMID: 33179792 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 12, 2020 Category: Hematology Authors: Liu A, Dhaliwal N, Upreti H, Kasmani J, Dane K, Moliterno A, Braunstein E, Brodsky R, Chaturvedi S Tags: Transfusion Source Type: research

Two novel FUT1 alleles that cause para-Bombay phenotype in a Chinese individual.
CONCLUSION: Two novel FUT1 alleles, with the previously not reported mutations c.508dupT and c.787C, respectively, are responsible for the para-Bombay phenotype detected in the sample from the proband. PMID: 33175455 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 11, 2020 Category: Hematology Authors: Lin FQ, Zhang KL, Li XF, Zhang X, Liu Y, Li JP Tags: Transfusion Source Type: research

Buffy coat platelets coming to America: Are we ready?
CONCLUSIONS: Buffy coat blood products are widely used throughout the world. Recent conversion from PRP to BC by CBS showed that conversion can be accomplished with planning, communication, and partnership from all stakeholders. In conclusion, BC PLTs are worth serious consideration in the United States, but regulatory barriers in the United States will need to be addressed. PMID: 33174258 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 10, 2020 Category: Hematology Authors: Gammon RR, Devine D, Katz LM, Quinley E, Wu Y, Rowe K, Razatos A, Min K, Reichenberg S, Smith R Tags: Transfusion Source Type: research

Fecal blood loss: A quantitative method of evaluating hemostasis in patients with thrombocytopenia.
CONCLUSION: A prophylactic platelet transfusion threshold of 5000/μL or greater is sufficient to maintain hemostasis in patients with thrombocytopenia. PMID: 33166412 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 9, 2020 Category: Hematology Authors: Slichter SJ, Gernsheimer T, LeBlanc R, Townsend-McCall D, Jones MK, Cuaron L, Bolgiano D Tags: Transfusion Source Type: research

Minor impact of patient alloantibodies against human platelet antigen (HPA)-15 in the effectiveness of platelet transfusion: A pilot study.
CONCLUSION: The efficacy of HPA-15-incompatible platelet transfusions was not necessarily inferior to that of HPA-15 compatible ones. Although the case number was limited, our results suggest that HPA-15 antibodies do not have a significant impact on the effects of platelet transfusion. PMID: 33166416 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 9, 2020 Category: Hematology Authors: Inoue H, Sakamoto R, Nishimiya H, Sakamoto H, Terasu S, Aminaka R, Koh Y, Takihara Y, Hirayama F, Kuroishi A Tags: Transfusion Source Type: research

Factor V activity in apheresis platelets: Implications for management of FV deficiency.
Abstract BACKGROUND: Allogeneic platelet (PLT) infusion is a strategy to raise Factor V (FV) levels in patients with congenital FV deficiency. However, since FV is labile in vitro, we hypothesized that FV activity could be low in PLT units. STUDY DESIGN AND METHODS: FV activity was tested using a prothrombin time-based platform in the supernatant and platelet lysate (PL) of apheresis PLT units (16 units stored in PLT additive solution with acetate and phosphate [PAS-C] and 10 units stored in plasma only), on post-collection days 3-6. Statistical analysis was performed using Student's t test (P 
Source: Transfusion - November 9, 2020 Category: Hematology Authors: Gupta GK, Hendrickson JE, Bahel P, Siddon AJ, Rinder HM, Tormey CA Tags: Transfusion Source Type: research

A comparison of platelet quality between platelets from healthy donors and hereditary hemochromatosis donors over seven-day storage.
Abstract BACKGROUND: Therapeutic phlebotomy is the standard treatment of hereditary hemochromatosis (HH), the most common genetic disease in people of Northern European descent. Red cell concentrates from HH donors have been reported safe for transfusion, but little data is available on the storage properties of platelet concentrates from HH donors. STUDY DESIGN AND METHODS: Whole blood was collected from 10 healthy individuals and 10 newly diagnosed HH patients with elevated serum ferritin. Platelet-rich plasma (PRP) was prepared and split into four 20-mL units. Platelet quality tests were performed o...
Source: Transfusion - November 9, 2020 Category: Hematology Authors: Mikaelsdottir M, Vidarsson B, Runarsson G, Bjarnadottir U, Onundarson PT, Sigurjonsson OE, Halldorsdottir AM Tags: Transfusion Source Type: research

Autoantigen Presentation by Splenic Dendritic Cells is Required for RBC-specific Autoimmunity.
CONCLUSION: These data suggest that if erythrocyte T cell tolerance fails, DCs are capable of initiating autoimmune responses. As such, targeting DCs may be a fruitful strategy for AIHA therapies. PMID: 33151564 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 5, 2020 Category: Hematology Authors: Richards AL, Qiu A, Dei Zotti F, Sheldon K, Usaneerungrueng C, Gruber DR, Hudson KE Tags: Transfusion Source Type: research

A novel A allele with c.963_964insC mutation identified in a Chinese individual.
PMID: 33146416 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 4, 2020 Category: Hematology Authors: Liu L, Chi X, Bu X, Feng Z, Su N Tags: Transfusion Source Type: research

Transfusion support in COVID-19 patients: Impact on hospital blood component supply during the outbreak.
CONCLUSION: Although only a minority of COVID-19 inpatients required transfusion, the expected reduction in transfusion needs caused by the lack of elective surgical procedures is partially offset by the large number of admitted patients during the peak of the pandemic. This fact must be taken into account when planning hospital blood supply. PMID: 33146420 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 4, 2020 Category: Hematology Authors: Velázquez-Kennedy K, Luna A, Sánchez-Tornero A, Jiménez-Chillón C, Jiménez-Martín A, Vallés Carboneras A, Tenorio M, García García I, López-Jiménez FJ, Moreno-Jiménez G Tags: Transfusion Source Type: research

Blood donor obesity is associated with changes in red blood cell metabolism and susceptibility to hemolysis in cold storage and in response to osmotic and oxidative stress.
Abstract BACKGROUND: Obesity is a global pandemic characterized by multiple comorbidities, including cardiovascular and metabolic diseases. The aim of this study was to define the associations between blood donor body mass index (BMI) and RBC measurements of metabolic stress and hemolysis. STUDY DESIGN AND METHODS: The associations between donor BMI (
Source: Transfusion - November 4, 2020 Category: Hematology Authors: Hazegh K, Fang F, Bravo MD, Tran JQ, Muench MO, Jackman RP, Roubinian N, Bertolone L, DʼAlessandro A, Dumont L, Page GP, Kanias T Tags: Transfusion Source Type: research

Identification of a novel c.94dupA mutation in RHD allele.
PMID: 33140446 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 3, 2020 Category: Hematology Authors: Deng D, Deng G, He Y, Yu Y Tags: Transfusion Source Type: research

Extension of platelet shelf life with an improved bacterial testing algorithm.
CONCLUSION: Screening of 7-day PCs increased bacterial detection mainly of anaerobes and reduced outdating. The incidence of septic transfusion events has decreased approximately threefold. A longer surveillance period is needed to evaluate the value of anaerobic cultures and residual safety risk. PMID: 33140420 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - November 2, 2020 Category: Hematology Authors: Ramirez-Arcos S, Evans S, McIntyre T, Pang C, Yi QL, DiFranco C, Goldman M Tags: Transfusion Source Type: research

SMIM1 missense mutations exert their effect on wild type Vel expression late in erythroid differentiation.
CONCLUSION: These results suggest that SMIM1 mutations exert a dominant negative effect on wtSMIM1 probably by affecting SMIM1 multimerization and thereby Vel epitope presentation at the latest stages of erythroid differentiation. PMID: 33128268 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 30, 2020 Category: Hematology Authors: van der Rijst MVE, Abay A, Aglialoro F, van der Schoot CE, van den Akker E Tags: Transfusion Source Type: research

Transfusion Reactions Associated with COVID-19 Convalescent Plasma Therapy for SARS-CoV-2.
CONCLUSION: Our findings support the use of CP as a safe therapeutic option from a transfusion reaction perspective, in the setting of COVID-19. Further studies are needed to confirm the clinical significance of ABO group B, age, and predisposing disease severity in the incidence of transfusion reaction events. PMID: 33125158 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 30, 2020 Category: Hematology Authors: Nguyen FT, van den Akker T, Lally K, Lam H, Lenskaya V, Liu STH, Bouvier NM, Aberg JA, Rodriguez D, Krammer F, Strauss D, Shaz BH, Rudon L, Galdon P, Jhang JS, Arinsburg SA, Baine I, Mount Sinai Health System Convalescent Plasma Team Tags: Transfusion Source Type: research

Validation of a SARS-CoV-2 RNA RT-PCR assay for high-throughput testing in blood of COVID-19 convalescent plasma donors and patients.
CONCLUSION: The SARS-CoV-2 RNA assay, only licensed for respiratory swabs, performed on a PCR system for high-throughput testing, showed a good assay performance for blood testing. PMID: 33125162 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 30, 2020 Category: Hematology Authors: Strasser EF, Steininger PA, Korn K, Achenbach S, Tenbusch M, Cunningham S, Zimmermann R, Überla K, Hackstein H Tags: Transfusion Source Type: research

Refractory thrombotic thrombocytopenic purpura related to checkpoint inhibitor immunotherapy.
CONCLUSION: This case illustrates not only that TTP may be a potential complication of checkpoint inhibitor therapy, but also that TTP developing in this setting may result in an unpredictable response to commonly employed TTP treatment modalities. Ultimately, checkpoint inhibitor-related TTP may require distinct management approaches and prognostic considerations. PMID: 33119913 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 29, 2020 Category: Hematology Authors: Lancelot M, Miller MJ, Roback J, Stowell SR Tags: Transfusion Source Type: research

"Camel humps"-shaped red blood cell histogram in a woman with extreme anemia treated with intravenous iron.
"Camel humps"-shaped red blood cell histogram in a woman with extreme anemia treated with intravenous iron. Transfusion. 2020 Oct 28;: Authors: Beverina I, Scalvini R, Brando B PMID: 33111350 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 28, 2020 Category: Hematology Authors: Beverina I, Scalvini R, Brando B Tags: Transfusion Source Type: research

Novel A4GALT gene variation with rare p phenotype in a compound heterozygous Chinese individual.
PMID: 33111355 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 28, 2020 Category: Hematology Authors: Chen Q, Xiao J, Duan Z, Shi L, Huang C, Li M Tags: Transfusion Source Type: research

In vitro thrombus formation and in vivo hemostasis mediated by platelets irradiated with bactericidal ultraviolet C from xenon flash under flow conditions.
CONCLUSIONS: PAS-PCs irradiated with UVC from xenon flash have favorable properties to achieve hemostasis compared with nonirradiated PAS-PCs. PMID: 33107611 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 27, 2020 Category: Hematology Authors: Abe H, Endo K, Nogawa M, Shiba M, Miyata S, Satake M Tags: Transfusion Source Type: research

A novel RHD allele caused by c.739 G > C mutation was identified in a Chinese individual.
A novel RHD allele caused by c.739 G> C mutation was identified in a Chinese individual. Transfusion. 2020 Oct 27;: Authors: Lyu H, Jiao S, Ma W, Hu B Abstract The blood sample of a pregnant Chinese woman of the north Han population was confirmed as D variation by serologic method, and subsequent detection of RHD Exons 1 through 10 by the polymerase chain reaction-sequence-specific primer test showed that all exons were present. To further clarify the molecular mechanism of this sample, we sequenced the RHD Exons 1 through 10 and found that it was a novel allele caused by c.739 G> C in Exon 5. ...
Source: Transfusion - October 27, 2020 Category: Hematology Authors: Lyu H, Jiao S, Ma W, Hu B Tags: Transfusion Source Type: research

Safety profile of high molecular weight polymerized hemoglobins.
CONCLUSION: Large MW PolybHbs were found to be safe and efficacious in increasing O2 carrying capacity and the O2 affinity of the PolybHb did not affect O2 delivery or extraction by tissues in relevant preclinical models. In conclusion, these results suggest that both T-state and R-state PolybHb are safe and do not impair O2 delivery. The results are encouraging and support further evaluation of high MW PolybHbs and their future feasibility compared to allogenic blood in a trauma model. PMID: 33104250 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 26, 2020 Category: Hematology Authors: Muller CR, Williams AT, Munoz CJ, Eaker AM, Breton AN, Palmer AF, Cabrales P Tags: Transfusion Source Type: research

Neutralizing and binding activities against SARS-CoV-1/2, MERS-CoV, and human coronaviruses 229E and OC43 by normal human intravenous immunoglobulin derived from healthy donors in Japan.
CONCLUSION: N-IVIG derived from healthy donors in Japan before the start of the COVID-19 pandemic had no direct effect against SARS-CoV-2. Further studies are warranted to determine the effects of N-IVIG manufactured after the start of the COVID-19 pandemic against SARS-CoV-2. PMID: 33104267 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 26, 2020 Category: Hematology Authors: Kubota-Koketsu R, Terada Y, Yunoki M, Sasaki T, Nakayama EE, Kamitani W, Shioda T Tags: Transfusion Source Type: research

Estimating the incidence of HIV infection in repeat blood donors with low average donation frequency.
CONCLUSION: The partial-case approach performed better than the whole-case approach. The conditions producing bias>10% are so extreme that they are unlikely to be encountered in the field. PMID: 33098135 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 24, 2020 Category: Hematology Authors: Brambilla DJ, Busch MP, Glynn SA, Kleinman SH, NHLBI Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) Tags: Transfusion Source Type: research

A novel allele arising from c.912C > A mutation in the α-1,3-N-acetylgalactosaminyltransferase gene in a Chinese individual.
CONCLUSION: A novel mutation c.912C>A in α-1,3-N-acetylgalactosaminyltransferase gene resulting in Am phenotype was identified in a Chinese individual. PMID: 33098315 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 24, 2020 Category: Hematology Authors: Zhang X, Li X, Liu Y, Li J Tags: Transfusion Source Type: research

SCAR: The high-prevalence antigen 013.008 in the Scianna blood group system.
CONCLUSIONS: We characterized a high-prevalence antigen, with the proposed name "SCAR," which is the eighth antigen of the Scianna blood group system (proposed designation 013.008). Individuals homozygous for ERMAP:p.(Gln142Glu) protein variant can produce anti-SCAR. Although we did not observe any sign of hemolysis at this time, the anti-SCAR prompted a change of the treatment regimen. A review of the known reports indicated that all SC alloantibodies of sufficient titer should be considered capable of causing hemolysis. PMID: 33098316 [PubMed - as supplied by publisher] (Source: Transfusion)
Source: Transfusion - October 24, 2020 Category: Hematology Authors: Srivastava K, Albasri J, Alsuhaibani OM, Aljasem HA, Bueno MU, Antonacci T, Branch DR, Denomme GA, Flegel WA Tags: Transfusion Source Type: research