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Total 656 results found since Jan 2013.

Systematic Comparisons of Formulations of Linear Oligolysine Peptides with siRNA and Plasmid DNA
This article is protected by copyright. All rights reserved. Linear oligolysine peptides with 16 or more lysines can effectively encapsulate plasmid DNA (pDNA) into monodisperse nanoparticles for gene transfer into the cells. However, the linear oligolysine peptides are not capable of forming monodisperse complexes with siRNA, hence they are ineffective in siRNA delivery into cells. Since the linear peptides have a limited rotation freedom, they are more suitable for cellular delivery of pDNA which has a higher rotation freedom than the more physically rigid siRNA.
Source: Chemical Biology and Drug Design - December 18, 2015 Category: Biology Authors: Albert Kwok, David McCarthy, Stephen L. Hart, Aristides D. Tagalakis Tags: Research Article Source Type: research

PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart.
Abstract The remarkable regenerative capacity of the zebrafish has made it an important model organism for studying heart regeneration. However, current loss-of-function studies are limited by a lack of conditional-knockout and effective gene-knockdown methods for the adult heart. Here, we report a novel siRNA knockdown method facilitated by poly (ethylene glycol)-b-poly (D, l-lactide) (PEG-PLA) nanoparticles. The siRNA-encapsulated nanoparticles successfully entered cells and resulted in remarkable gene-specific knockdown in the adult heart. This effect was demonstrated by down-regulation of the Aldh1a2 and Dusp6...
Source: Developmental Biology - August 29, 2015 Category: Biology Authors: Diao J, Wang H, Chang N, Zhou XH, Zhu X, Wang J, Xiong JW Tags: Dev Biol Source Type: research

The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects
by Piotr J. Kamola, Yuko Nakano, Tomoko Takahashi, Paul A. Wilson, Kumiko Ui-Tei RNA interference (RNAi) is a powerful tool for post-transcriptional gene silencing. However, the siRNA guide strand may bind unintended off-target transcripts via partial sequence complementarity by a mechanism closely mirroring micro RNA (miRNA) silencing. To better understand these off-target effects, we investigated the correlation between sequence features within various subsections of siRNA guide strands, and its corresponding target sequences, with off-target activities. Our results confirm previous reports that strength of base-pairing...
Source: PLoS Computational Biology - December 11, 2015 Category: Biology Authors: Piotr J. Kamola et al. Source Type: research

Novel polyacrylate-based cationic nanoparticles for survivin siRNA delivery combined with mitoxantrone for treatment of breast cancer
Publication date: Available online 3 October 2016 Source:Biologicals Author(s): Sanam Arami, Majid Mahdavi, Mohammad Reza Rashidi, Marziyeh Fathi, Mohammad-saeid Hejazi, Nasser Samadi As a gene delivery method in breast cancer therapy, knocking down the undesired genes in the cancerous cells would be promising. Inhibitors of Apoptosis Protein (IAP) family genes are some of the genes whose responsibility is inhibition of apoptosis in cells. Silencing these genes seems to be helpful directing the tumor cells to death. siRNA sequence designed against survivin anti-apoptotic gene can play this role if carried to the cytoplasm...
Source: Biologicals - October 3, 2016 Category: Biology Source Type: research

Therapeutic potential of chemically modified siRNA: Recent trends
This article is protected by copyright. All rights reserved. Ribose Modifications Phosphate Backbone Modifications Nucleobase Modifications Modification to the Termini and Conjugate groups siRNA Binding
Source: Chemical Biology and Drug Design - April 5, 2017 Category: Biology Authors: Chelliah Selvam, Daniel Mutisya, Sandhya Prakash, Kasturi Ranganna, Ramasamy Thilagavathi Tags: Review Source Type: research

Specific Delivery of Delta-5-Desaturase siRNA via RNA Nanoparticles Supplemented with Dihomo-γ-Linolenic Acid for Colon Cancer Suppression
In this study, we employed RNA nanotechnology for specific delivery of D5D-siRNA to xenograft colon tumors using 3WJ RNA nanoparticles. When a targeting module, i.e., the EpCAM aptamer, was incorporated, the 3WJ pRNA nanoparticles were able specifically deliver D5D siRNA to human colon cancer HCA-7 cells both in vitro and in vivo, resulting in significant downregulation of D5D expression. Co-treatment with DGLA in combination with 3WJ-EpCAM-siRNA induced a higher DGLA/AA ratio and enhanced formation of 8-HOA at a threshold level, and in HCA-7 tumor-bearing mice, induced significant tumor suppression. We further confirmed t...
Source: Redox Biology - December 19, 2018 Category: Biology Source Type: research

MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation.
Abstract More than 2,000 C. elegans genes are targeted for RNA silencing by the mutator complex, a specialized small interfering RNA (siRNA) amplification module which is nucleated by the Q/N-rich protein MUT-16. The mutator complex localizes to Mutator foci adjacent to P granules at the nuclear periphery in germ cells [1]. Here, we show that the DEAD box RNA helicase smut-1 functions redundantly in the mutator pathway with its paralog mut-14 during RNAi. Mutations in both smut-1 and mut-14 also cause widespread loss of endogenous siRNAs. The targets of mut-14 and smut-1 largely overlap with the targets of other ...
Source: Current Biology - March 26, 2014 Category: Biology Authors: Phillips CM, Montgomery BE, Breen PC, Roovers EF, Rim YS, Ohsumi TK, Newman MA, van Wolfswinkel JC, Ketting RF, Ruvkun G, Montgomery TA Tags: Curr Biol Source Type: research

Effect of siRNA PERK on Fluoride-Induced Osteoblastic Differentiation in OS732 Cells.
This study proved that the mechanism underlying fluoride induced osteoblastic and osteoclastic differentiation possible was due to activation of ALP and RANKL mediated by PERK in OS732 cells. PMID: 24838929 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - May 18, 2014 Category: Biology Authors: Lü P, Li X, Ruan L, Xu H, Liu Q Tags: Biol Trace Elem Res Source Type: research

siRNA transfection in the barnacle Amphibalanus amphitrite larvae METHODS amp TECHNIQUES
In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either nauplius or cyprid stage, or both. Effects of siRNA transfection on p38 MAPK level were hardly detectable in the cyprids that their corresponding nauplii were transfected. In contrast, larvae that were transfected at cyprid stage showed lower level of p38 MAPK than the blank and reagent controls. However, significantly decreased level of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rate were observed only in the "Double Transfection", in which larvae were exposed to siRNA solution at both the naupliu...
Source: Journal of Experimental Biology - June 25, 2015 Category: Biology Authors: Zhang, G., He, L.-s., Wong, Y. H., Yu, L., Qian, P.-y. Tags: METHODS [amp ] TECHNIQUES Source Type: research

siRNA transfection in larvae of the barnacle Amphibalanus amphitrite METHODS amp TECHNIQUES
In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to s...
Source: Journal of Experimental Biology - August 19, 2015 Category: Biology Authors: Zhang, G., He, L.-S., Wong, Y. H., Yu, L., Qian, P.-Y. Tags: METHODS [amp ] TECHNIQUES Source Type: research

siRNA ‐loaded liposomes: inhibition of encystment of Acanthamoeba and toxicity on the eye surface
This article is protected by copyright. All rights reserved. Glycogen phosphorilase, a key enzyme for encystment of Acanthamoeba,was silenced PEGylated cationic liposomes were used as carriers for siRNA delivery Ther was a significant reduction in the encystment of AP2 trophozoties
Source: Chemical Biology and Drug Design - January 29, 2017 Category: Biology Authors: Kathrin Faber, Giovanni K. Zorzi, Nathaly T. Brazil, Marilise B. Rott, Helder F. Teixeira Tags: Research Article Source Type: research

A Bifurcated Proteoglycan Binding Small Molecule Carrier for siRNA delivery
This article is protected by copyright. All rights reserved. A bifurcated guanidylated small molecule is modelled to access different clusters of sulphate esters in proteoglycans bound to the cell surface. Modular synthesis gives ready access to these molecules which have potential for the delivery of siRNA.
Source: Chemical Biology and Drug Design - January 28, 2014 Category: Biology Authors: Matt Gooding, Derick Adigbli, A. W. Edith Chan, Roberta J. Melander, Alexander J. MacRobert, David L. Selwood Tags: Research Article Source Type: research

Ancient Endo-siRNA Pathways Reveal New Tricks.
Abstract Endogenously produced small interfering RNAs (endo-siRNAs, 18-30 nucleotides) play a key role in gene regulatory pathways, guiding Argonaute effector proteins as a part of a functional ribonucleoprotein complex called the RISC (RNA induced silencing complex) to complementarily target nucleic acid. Enabled by the advent of high throughput sequencing, there has been an explosion in the identification of endo-siRNAs in all three kingdoms of life since the discovery of the first microRNA in 1993. Concurrently, our knowledge of the variety of cellular processes in which small RNA pathways related to RNA interf...
Source: Current Biology - August 4, 2014 Category: Biology Authors: Claycomb JM Tags: Curr Biol Source Type: research

TLR4 siRNA inhibits proliferation and invasion in colorectal cancer cells by downregulating ACAT1 expression
Publication date: Available online 10 May 2016 Source:Life Sciences Author(s): Kai Ye, Yiyang Wu, Yafeng Sun, Jian'an Lin, Jianhua Xu Aims Toll-like receptor 4 (TLR4) is involved in tumor development. Numerous studies have confirmed that TLR4 mediates processes in tumorigenesis, for example, inflammation, proliferation and invasion. However, the effects of TLR4 on colorectal cancer development have not been fully elucidated. The present study aimed to evaluate the effects and mechanisms of TLR4 on colorectal cancer development. Main methods The expression of TLR4 and Acyl coenzyme A cholesterol acyltransferase 1 (A...
Source: Life Sciences - May 10, 2016 Category: Biology Source Type: research

Application of bifurcation theory and siRNA-based control signal to restore the proper response of cancer cells to DNA damage
Publication date: 7 November 2016 Source:Journal of Theoretical Biology, Volume 408 Author(s): Emilia Kozłowska, Krzysztof Puszynski Many diseases with a genetic background such as some types of cancer are caused by damage in the p53 signaling pathway. The damage changes the system dynamics providing cancer cells with resistance to therapy such as radiation therapy. The change can be observed as the difference in bifurcation diagrams and equilibria type and location between normal and damaged cells, and summarized as the changes of the mathematical model parameters and following changes of the eigenvalues of Jacobian mat...
Source: Journal of Theoretical Biology - August 23, 2016 Category: Biology Source Type: research