TLR4 siRNA inhibits proliferation and invasion in colorectal cancer cells by downregulating ACAT1 expression

Publication date: Available online 10 May 2016 Source:Life Sciences Author(s): Kai Ye, Yiyang Wu, Yafeng Sun, Jian'an Lin, Jianhua Xu Aims Toll-like receptor 4 (TLR4) is involved in tumor development. Numerous studies have confirmed that TLR4 mediates processes in tumorigenesis, for example, inflammation, proliferation and invasion. However, the effects of TLR4 on colorectal cancer development have not been fully elucidated. The present study aimed to evaluate the effects and mechanisms of TLR4 on colorectal cancer development. Main methods The expression of TLR4 and Acyl coenzyme A cholesterol acyltransferase 1 (ACAT1) in colorectal cancer and cell lines was detected using RT-PCR and Western blot. The nude mouse xenograft model was established by subcutaneous injection of SW480 cells transfected with TLR4 scramble or TLR4 siRNA. CCK8 and transwell assays were used to evaluate the effects of TLR4 silencing on cell proliferation, migration and invasion in HT29 and SW480. RT-PCR and Western blot was used to determine the regulation between TLR4 and ACAT1. Key findings Both TLR4 and ACAT1 were highly expressed in colorectal cancer tissues and cell lines. Inhibition of TLR4 reduced tumor growth suppressed cell proliferation, migration and invasion in HT29 and SW480. TLR4 siRNA could decrease ACAT1 expression in HT29, and that overexpression of ACAT1 by pLV-Neo-ACAT1 abolished the effect of TLR4 on colorectal cancer cell lines. Significance TLR4 siRNA inhibits ...
Source: Life Sciences - Category: Biology Source Type: research