Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress
Publication date: Available online 22 February 2020Source: Life SciencesAuthor(s): Rahmani Mohammad Malyar, Hu Li, Dandan Liu, Yassin Abdulrahim, Rawan Ahmad Farid, Fang Gan, Waseem Ali, Hamdard Enayatullah, Sayed Attaul Haq Banuree, Kehe Huang, Xingxiang ChenAbstractAimsThe present study was carried out to investigate the influences of Selenium/Zinc-Enriched probiotics (SeZnP) on growth performance, serum enzyme activity, antioxidant capability, inflammatory factors and gene expression associated with Wistar rats inflated under high ambient thermal-stress.Main methodsSixty male rates with six-weeks of age were randomly al...
Source: Life Sciences - February 23, 2020 Category: Biology Source Type: research

New insights into the zinc-α2-glycoprotein (ZAG) scaffold and its metal ions binding abilities using spectroscopic techniques
In this study, fluorescence emission spectroscopy and mass spectrometry (MALDI-TOF) were employed to define the putative interaction sites and their accessibility for the biologically important metals of Irving William Series.Key findingsSeveral hotspot residues in the ZAG scaffold involved in these interactions were mapped and their binding affinity score for each metal has been determined. Their binding abilities of these sites and aggregation propensities of ZAG were monitored by fluorescence emission spectroscopy.SignificanceThe prediction of such binding affinity with metals on the active sites and its impact on the c...
Source: Life Sciences - February 23, 2020 Category: Biology Source Type: research

Cellular context- and protein level-dependent interaction of pluripotency factor OCT4A with multiple octamer motifs of the same target gene
Publication date: Available online 22 February 2020Source: Life SciencesAuthor(s): Ning Zhou, Xiao-Bing Zhang, Cheng Chen, Xin-Yu Chen, Bo Kang, Jian-Qin He, Guo-Zhong Gong, Ying-Jie Wang, Yan-Wen ZhouAbstractAimsTo compare how OCT4A proteins interact with and regulate multiple OCT4A-octamer motifs (OMs) in different regions of the FOS gene expressed in somatic cancer cells versus pluripotent stem cells.Materials and methodsTwo FOS reporter gene systems harboring predicted OMs or their mutational counterparts were introduced into HeLa and NCCIT cells with varying OCT4A protein levels. The transcription of dsGFP reflecting ...
Source: Life Sciences - February 23, 2020 Category: Biology Source Type: research

Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing
In this study, we will investigate the therapeutic effects of berberine (BBR) in Helicobacter pylori (H. pylori) induced chronic atrophic gastritis (CAG). Furthermore, potential mechanisms of BBR in regulating IRF8-IFN-γ signaling axis will also be investigated.Materials and methodsH. pylori were utilized to establish CAG model of rats. Therapeutic effects of BBR on serum supernatant indices, and histopathology of stomach were analyzed in vivo. Moreover, GES-1 cells were infected by H. pylori, and intervened with BBR in vitro. Cell viability, morphology, proliferation, and quantitative analysis were detected by high-...
Source: Life Sciences - February 23, 2020 Category: Biology Source Type: research

A review on anti-cancer properties of Quercetin in breast cancer
Publication date: Available online 22 February 2020Source: Life SciencesAuthor(s): Maryam Ezzati, Bahman Yousefi, Kobra Velaei, Amin SafaAbstractBreast cancer is one of the well-known malignant tumors among women. In spite of attempts to classifying breast cancer according to its histological and molecular properties, it is almost considered as a dilemma in treatment. Nowadays, public and medical attentions have primary focused on foods with anti-cancer properties to alleviate the cancer problems. Flavonoid components such as Quercetin (Qu) as dietary substances with high attention of ordinary people might provide potentia...
Source: Life Sciences - February 23, 2020 Category: Biology Source Type: research

Melatonin induces mitochondrial apoptosis in osteoblasts by regulating the STIM1/cytosolic calcium elevation/ERK pathway
This study aimed to determine the mechanism by which melatonin inhibits the proliferation of osteoblasts.Main methodsCell viability experiment, DNA fragment detection and alkaline phosphatase (ALP) activity assays were performed to determine the effects of melatonin on the proliferation, apoptosis and differentiation of osteoblasts. We used immunofluorescence to detect the expression of STIM1 in melatonin-treated osteoblasts. STIM1 interference was achieved using a specific siRNA, and a TRPC inhibitor was used to block the influx of Ca2+. The mRNA expression was determined by RT-qPCR, and protein levels were measured by We...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Dihydroartemisinin inhibits the tumorigenesis and metastasis of breast cancer via downregulating CIZ1 expression associated with TGF-β1 signaling
In this study, we aimed to investigate the anti-proliferative and anti-metastasis effects of DHA.Main methodsUtilizing breast cancer cells MCF-7, MDA-MB-231 and BT549, cell proliferation, migration and invasion were detected. RT-qPCR was performed to detect CIZ1, TGF-β1 and Snail expression, and the interactions of these related molecules were analyzed by GeneMANIA database. Western blot detected CIZ1, TGF-β1/Smads signaling and Snail expression in DHA-treated cells, in TGFβ1-induced cells with enhanced metastatic capacity, and in cells treated with DHA plus TGFβ1/TGFβ1 inhibitor SD-208.Key finding...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Sin1 promotes proliferation and invasion of prostate Cancer cells by modulating mTORC2-AKT and AR signaling cascades
Publication date: Available online 21 February 2020Source: Life SciencesAuthor(s): Yunchuanxiang Huang, Guanying Feng, Jingshu Cai, Qian Peng, Zhenglin Yang, Chunhong Yan, Lu Yang, Ziyan WangAbstractAimsProstate cancer (PCa) is the most common type of cancer and a major cause of death in men worldwide. Aberrant Androgen receptor (AR) and PI3K-AKT signaling are very frequent in PCa patients and, therefore, considered as therapeutic targets in the clinic. Sin1 is an essential component of mTORC2 complex, which determines full AKT activation and PCa development in PTEN−/− mice. Here we examined the role of Sin1 in...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

New combination therapy of gliclazide and quercetin for protection against STZ-induced diabetic rats
Publication date: Available online 21 February 2020Source: Life SciencesAuthor(s): Noha F. Abdelkader, Hebatollah E. Eitah, Yousreya A. Maklad, Amina A. Gamaleldin, Manal A. Badawi, Sanaa A. KenawyAbstractAimsThe use of natural agents with anti-diabetic effect in combination therapy adds further positive clinical implications in the management of diabetes mellitus. Interestingly, quercetin is one of the most potent naturally occurring antioxidant which possesses various pharmacological actions including anti-diabetic effect. Thus, this research was conducted to assess the efficiency of a new combination from gliclazide and...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Multiple sevoflurane exposures don't disturb the T-cell receptor repertoire in infant rhesus monkeys' thymus
Publication date: Available online 21 February 2020Source: Life SciencesAuthor(s): Yanyong Cheng, Jie Wang, Niming Wu, Lei Zhang, Hong JiangAbstractAimsMultiple surgical procedures and anesthesia increase the risk of the development in children. However, the influence of such exposures on the developing childhood immunity organs is rarely reported.Materials and methodsHigh-throughput sequencing of T-cell receptor (TCR) repertoires (TCRseq) from rhesus monkeys' thymus was performed to investigate whether anesthetics could induce de novo antigen recognition via TCR or TCR development impairments.Key findingsNo significant di...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Schwann cells apoptosis is induced by high glucose in diabetic peripheral neuropathy
Publication date: Available online 21 February 2020Source: Life SciencesAuthor(s): Yu-pu Liu, Shui-jin Shao, Hai-dong GuoAbstractDiabetic peripheral neuropathy (DPN) is a common complication of diabetes mellitus that affects approximately half of patients with diabetes. Current treatment regimens cannot treat DPN effectively. Schwann cells (SCs) are very sensitive to glucose concentration and insulin, and closely associated with the occurrence and development of type 1 diabetic mellitus (T1DM) and DPN. Apoptosis of SCs is induced by hyperglycemia and is involved in the pathogenesis of DPN. This review considers the patholo...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Carbon nanotubes functionalized with sodium hyaluronate: Sterilization, osteogenic capacity and renal function analysis
This study determined the optimum gamma irradiation dosage to sterilize sodium hyaluronate (HY), single-walled carbon nanotubes (SWCNT), multi-walled carbon nanotubes (MWCNT) and CNT functionalized with HY (HY-SWCNT and HY-MWCNT), evaluated the structural integrity of the materials and assessed whether sterilized materials kept biological properties without affecting renal function.Main methodsMaterials were submitted to dosages of 100 gγ to 30 Kgγ and plated onto agar mediums for colony forming units (CFUs) counting. Sterilized samples were inoculated with 107 Bacillus clausii, submitted again to gamma irradia...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Corrigendum to “Effect of resveratrol combined with atorvastatin on re-endothelialization after drug-eluting stents implantation and the underlying mechanism” [Life Sci. 245 (2020) 117349]
Publication date: 15 April 2020Source: Life Sciences, Volume 247Author(s): Changzhe Chen, Chenxi Song, Dong Zhang, Dong Yin, Rui Zhang, Jingzhou Chen, Kefei Dou (Source: Life Sciences)
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Early astragaloside IV administration attenuates experimental autoimmune encephalomyelitis in mice by suppressing the maturation and function of dendritic cells
Publication date: Available online 19 February 2020Source: Life SciencesAuthor(s): Liu Yang, Xinyan Han, Jinfeng Yuan, Faping Xing, Zhixing Hu, Fei Huang, Hui Wu, Hailian Shi, Ting Zhang, Xiaojun WuAbstractAimsDendritic cells (DCs) actively participate in the pathogenesis of multiple sclerosis (MS), an autoimmune disease. Astragaloside IV (ASI), an active monomer isolated from the Chinese medicine Astragalus membranaceus, has a wide range of pharmacological effects. We aimed to elucidate the effects of ASI on the development of DCs in the early stage of MS/EAE.Main methodsThe mice were administered with ASI (20 mg/kg) da...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Whole body hypothermia extends tissue plasminogen activator treatment window in the rat model of embolic stroke
Publication date: Available online 20 February 2020Source: Life SciencesAuthor(s): Mahsa Hassanipour, Mohammadreza Zarisfi, Vahid Ehsani, Mohammad AllahtavakoliAbstractLate treatment with tissue plasminogen activator (tPA) leads to reperfusion injury and poor outcome in ischemic stroke. We have recently shown the beneficial effects of local brain hypothermia after late thrombolysis. Herein, we investigated whether transient whole-body hypothermia was neuroprotective and could prevent the side effects of late tPA therapy at 5.5 h after embolic stroke. After induction of stroke, male rats were randomly assigned into four g...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Electroacupuncture preconditioning attenuates acute myocardial ischemia injury through inhibiting NLRP3 inflammasome activation in mice
Publication date: Available online 20 February 2020Source: Life SciencesAuthor(s): Tao Zhang, Wen-xiu Yang, Ya-ling Wang, Jing Yuan, Yi Qian, Qin-mei Sun, Mei-ling Yu, Shu-ping Fu, Bin Xu, Sheng-feng LuAbstractAimsElectro-acupuncture pretreatment (EAP) plays a protective role in myocardial ischemia (MI) injury. However, the underlying mechanism remains unclear. A growing body of evidence suggests postinfarction inflammatory response directly affects the remodeling of ventricular function. The purpose of this study was to investigate whether EAP alleviates MI through NLRP3 inflammasome inhibition.Materials and methodsWe con...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Tropisetron protects against brain aging via attenuating oxidative stress, apoptosis and inflammation: The role of SIRT1 signaling
Publication date: Available online 20 February 2020Source: Life SciencesAuthor(s): Atefeh Mirshafa, Hamidreza Mohammadi, Mohammad Shokrzadeh, Ebrahim Mohammadi, Fereshteh Talebpour Amiri, Fatemeh ShakiAbstractAimsThe aim of this study was to elucidate the signaling pathway involved in the anti-aging effect of tropisetron and to clarify whether it affects mitochondrial oxidative stress, apoptosis and inflammation in the aging mouse brain by upregulating Sirtuin 1 or silent information regulator 1 (SIRT1).Materials and methodsAging was induced by d-galactose (DG) at the dose of 200 mg/kg body weight/day subcutaneously inje...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Clearing or subverting the enemy: Role of autophagy in protozoan infections
Publication date: Available online 20 February 2020Source: Life SciencesAuthor(s): George Ghartey-Kwansah, Benjamin Aboagye, Frank Adu-Nti, Yeboah Kwaku Opoku, Emmanuel Kwasi AbuAbstractThe protozoan parasites are evolutionarily divergent, unicellular eukaryotic pathogens representing one of the essential sources of parasitic diseases. These parasites significantly affect the economy and cause public health burdens globally. Protozoan parasites share many cellular features and pathways with their respective host cells. This includes autophagy, a process responsible for self-degradation of the cell's components. There is co...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Modification of lung endoplasmic reticulum genes expression and NF-kB protein levels in obese ovalbumin-sensitized male and female rats
In this study, we investigated the expression of endoplasmic reticulum (ER) stress genes in the lung tissue of obese ovalbumin (OVA)-sensitized male and female rats.Main methodsThe rats were divided into eight groups (n = 5 per group) as follows: female and male rats fed with normal diet (FND and MND, respectively), female and male OVA-sensitized rats fed with normal diet (F-OND and M-OND, respectively), female and male rats fed with high-fat diet (F-HFD and M-HFD, respectively), female and male OVA-sensitized rats fed with high-fat diet (F-OHFD and M-OHFD, respectively). All rats were fed with a high-fat diet or stand...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Free and hydrogel encapsulated exosome-based therapies in regenerative medicine
Publication date: Available online 19 February 2020Source: Life SciencesAuthor(s): Ali Akbari, Nassrollah Jabbari, Roholah Sharifi, Mahdi Ahmadi, Ali Vahhabi, Seyyed Javad Seyedzadeh, Muhammad Nawaz, Sławomir Szafert, Monireh Mahmoodi, Esmaiel Jabbari, Rahim Asghari, Jafar RezaieAbstractOver the last few decades, mesenchymal stem cells-derived exosomes (MSCs-Ex) have attracted a lot of attention as a therapeutic tool in regenerative medicine. Exosomes are extracellular vehicles (EVs) that play important roles in cell-cell communication through various processes such as stress response, senescence, angiogenesis, and cell d...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

HOTTIP knockdown inhibits cell proliferation and migration via regulating miR-490-3p/HMGB1 axis and PI3K-AKT signaling pathway in ox-LDL-induced VSMCs
Publication date: Available online 19 February 2020Source: Life SciencesAuthor(s): Xiaoyan Guo, Yuhao Liu, Xiaohui Zheng, Yan Han, Jiangtao ChengAbstractAimsAtherosclerosis (AS) is a common cardiovascular disease with complicated pathogenesis. Long non-coding RNAs (lncRNAs) have been reported to be associated with AS progression. We aimed to explore the role and underlying mechanism of HOXA transcript at the distal tip (HOTTIP) in AS.Materials and methodsQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of HOTTIP, miR-490-3p and high mobility group B 1 (HMGB1) in AS patients'...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Proteome array of antibody responses to Chlamydia trachomatis infection in nonhuman primates
This study aimed to systemically profile antibody responses to C. trachomatis infection in nonhuman primates.Materials and methodsSera were obtained from 4 pig-tailed and 8 long-tailed macaques which were intravaginally or ocularly infected with live C. trachomatis organisms, and analyzed by C. trachomatis proteome array of antigens.Key findingsThe sera from 12 macaques recognized total 172 C. trachomatis antigens. While 84 antigens were recognized by pig-tailed macaques intravaginally infected with serovar D strain, 125 antigens were recognized by long-tailed macaques ocularly infected with serovar A, and 37 antigens were...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

The mechanisms of lysophosphatidylcholine in the development of diseases
Publication date: Available online 19 February 2020Source: Life SciencesAuthor(s): Panpan Liu, Wu Zhu, Chao Chen, Bei Yan, Lei Zhu, Xiang Chen, Cong PengAbstractLysophosphatidylcholine (LPC) is the main component of oxidatively damaged low-density lipoprotein (oxLDL). LPC originates from the cleavage of phosphatidylcholine by phospholipase A2 (PLA2). LPC plays a biological role by binding to G protein-coupled receptors and Toll-like receptors. LPC can induce the migration of lymphocytes and macrophages, increase the production of pro-inflammatory cytokines, induce oxidative stress, and promote apoptosis, which can aggregat...
Source: Life Sciences - February 20, 2020 Category: Biology Source Type: research

Circadian clock gene Clock is involved in the pathogenesis of preeclampsia through hypoxia
ConclusionThe expression of Clock gene in human placenta tissue, hypoxia cell model and RUPP rat model suggests that it may regulate the biological behavior of trophoblast cells through hypoxia, and then participate in the pathogenesis of preeclampsia. (Source: Life Sciences)
Source: Life Sciences - February 17, 2020 Category: Biology Source Type: research

Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
Publication date: Available online 17 February 2020Source: Life SciencesAuthor(s): Raghunath Singh, Yashika Bansal, Rupinder Kaur Sodhi, Dhirendra Pratap Singh, Mahendra Bishnoi, Kanthi Kiran Kondepudi, Bikash Medhi, Anurag KuhadAbstractTransient receptor potential vanilloid type 1 (TRPV1) channels are emerging therapeutic targets for metabolic disorders. Berberine, which is a modulator of TRPV1, has proven antiobesity and antidiabetic potentials. The present study was aimed to investigate the protective effects of berberine in olanzapine-induced alterations in hypothalamic appetite control, inflammation and metabolic aber...
Source: Life Sciences - February 17, 2020 Category: Biology Source Type: research

The role of Toll-like receptor 4 signaling pathway in ovarian, cervical, and endometrial cancers
Publication date: Available online 17 February 2020Source: Life SciencesAuthor(s): Luiz Antonio Lupi, Maira Smaniotto Cucielo, Henrique Spaulonci Silveira, Letícia Barbosa Gaiotte, Roberta Carvalho Cesário, Fábio Rodrigues Ferreira Seiva, Luiz Gustavo de Almeida ChuffaAbstractToll-like receptors (TLRs) are critical sensors related to inflammation and tumorigenesis. Among all subtypes, the TLR4 is a highly described transmembrane protein involved in the inflammatory process. The TLR4/myeloid differentiation factor 88 (MyD88) signaling pathway has been implicated in oncogenic events in several tissues an...
Source: Life Sciences - February 17, 2020 Category: Biology Source Type: research

FOXO4 expression associates with glioblastoma development and FOXO4 expression inhibits cell malignant phenotypes in vitro and in vivo
In conclusion, FOXO4 possesses an anti-cancer glioma activity, which could be a novel target for future control of GBM. (Source: Life Sciences)
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

Fibroblast growth factor-inducible 14 mediates macrophage infiltration in heart to promote pressure overload-induced cardiac dysfunction
The objective of this study was to investigate the role of TWEAK/Fn14 pathway in promoting cardiac inflammation under non ischemic stress conditions.Main methodsWild type (WT) and Fn14 knock out (Fn14−/−) mice were subjected to pressure overload [transaortic constriction (TAC)] for 1 or 6 weeks. A subset of WT TAC animals were treated with the Fn14 antagonist L524-0366. Cardiac function was measured by echocardiography. Cardiac fibrosis and macrophage infiltration were quantified using immunohistochemistry and flow cytometry, respectively. Cardiac fibroblasts were isolated for quantifying TWEAK-induced chemok...
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

PD-L1/PD-1 axis as a potent therapeutic target in breast cancer
Publication date: Available online 16 February 2020Source: Life SciencesAuthor(s): Shima Bastaki, Mahzad Irandoust, Armin Ahmadi, Mohammad Hojjat-Farsangi, Patrick Ambrose, Shahin Hallaj, Mahdi Edalati Fathabad, Ghasem Ghalamfarsa, Gholamreza Azizi, Mehdi Yousefi, Hengameh Chalajour, Farhad Jadidi-NiaraghAbstractAlthough both the incidence and the mortality rate of breast cancer is rising, there is no potent and practical option for the treatment of these patients, particularly in advanced stages. One of the most critical challenges for treatment is the presence of complicated and extensive tumor escape mechanisms in the t...
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

Synthesis of novel caffeic acid derivatives and their protective effect against hydrogen peroxide induced oxidative stress via Nrf2 pathway
This study was aimed to synthesize novel caffeic acid derivatives and evaluate their potential applications for the treatment of oxidative stress associated disease.Main methodsCaffeic acid sulfonamide derivatives were synthesized by coupling sulfonamides to the backbone of caffeic acid and fully characterized by melting points test, FT-IR, MS, NMR, UV–vis and n-octanol–water distribution assay. Their free radical scavenging ability was evaluated using DPPH assay and cytotoxicity against A549 cells were determined by MTT assay. The protective effect of these derivatives against hydrogen peroxide (H2O2) induced ...
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

Exogenous melatonin restrains neuroinflammation in high fat diet induced diabetic rats through attenuating indoleamine 2,3-dioxygenase 1 expression
Publication date: Available online 15 February 2020Source: Life SciencesAuthor(s): Adham M. Maher, Samar R. Saleh, Nihal M. Elguindy, Hagar M. Hashem, Galila A. YacoutAbstractAim of the workNeuroinflammation can arise from metabolic disturbances accompanying type 2 diabetes mellitus (T2DM) with an implication of indoleamine 2,3-dioxygenase 1 (IDO1). The antioxidant and anti-inflammatory potentials of melatonin (Mel) can amend diabetic complications. Here, we examined the effect of exogenous melatonin on neuroinflammation in high fat diet (HFD)-induced T2DM rats.Main methodsTwenty-one adult male Sprague-dawley rats were div...
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

Hypoxia-induced USP22-BMI1 axis promotes the stemness and malignancy of glioma stem cells via regulation of HIF-1α
This study intends to investigate the mechanisms of ubiqutin-specific protease 22 (USP22)/B cell-specific Moloney murine leukemia virus integration site 1 (BMI1) on the biological phenotypes of glioma stem cells (GSCs) under hypoxia.Main methodsWestern blot, Cell Counting Kit-8, colony formation and flow cytometry assays were preformed to evaluate cells biological behaviors. Luciferase assay was utilized to identify the associations among USP22, HIF-1α and BMI1.Key findingsSilencing USP22 reduced the stemness and proliferation of GSCs, and increased its apoptosis in response to hypoxia. Whilst, overexpression of BMI1...
Source: Life Sciences - February 16, 2020 Category: Biology Source Type: research

Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats
ConclusionUrantide can effectively block the UII/GPR14 system by regulating the JAK2/STAT3 signaling pathway to prevent and treat atherosclerosis-related kidney injury. At this stage, effective inhibition of inflammatory signaling pathways is of great significance in the treatment of atherosclerosis.Graphical abstract (Source: Life Sciences)
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Is CD47 a potentially promising therapeutic target in cardiovascular diseases? — Role of CD47 in cardiovascular diseases
In conclusion, these observations suggest a pathogenic role of CD47 in the development of cardiovascular diseases (CVDs) and indicate that CD47 might be a potentially promising molecular target for treating CVDs. Herein, we highlight the role of CD47 in the CVD pathogenesis and discuss the potential clinical application by targeting CD47 for treating CVDs.Graphical abstract (Source: Life Sciences)
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Effects of prenatal cold stress on maternal serum metabolomics in rats
In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after cold stress of 3 or 7 days. The results showed that 19 metabolites in the CS (cold stress 7 days)-NS (control) group and 23 metabolites in the CT (cold stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal cold stress ...
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Angiopoietin-1 accelerates Alzheimer's disease via FOXA2/PEN2/APP pathway in APP/PS1 mice
In conclusion, Ang-1 has an accelerative effect on Alzheimer's disease by increasing the secretion of Aβ42 via FOXA2/PEN2/APP pathway. (Source: Life Sciences)
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway
In this study, we investigated the mechanism through which melatonin inhibits apoptosis in mouse Leydig cells by activating the retinoic acid-related orphan nuclear receptor (ROR) α/p53 signaling pathway. We confirmed the expression and localization of RORα in mouse Leydig cells using immunofluorescence. After treatment with 10 ng/mL melatonin for 36 h, RORα mRNA and protein levels were significantly increased (P 
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Clopidogrel or Prasugrel reduces mortality and lessens cardiovascular damage from acute myocardial infarction in Hypercholesterolemic male rats
Publication date: Available online 13 February 2020Source: Life SciencesAuthor(s): Hala M.F. Mohammad, Samy M. Makary, Hoda Atef, Mohamed El-Sherbiny, Hebatallah H. Atteia, Gehan A. Ibrahim, Abdelaty Shawky Mohamed, Sawsan A. ZaitoneAbstractAimsHypercholesterolemia is a hazard for increasing susceptibility of the heart to myocardial infarction (MI) by inducing platelet hyperaggregability. Clopidogrel and prasugrel have documented cardioprotective effects in clinical studies. Herein, we investigated whether clopidogrel and prasugrel could protect against isoproterenol-induced acute MI (A-MI) under hypercholesterolemic condi...
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Beneficial effects of tamoxifen on leptin sensitivity in young mice fed a high fat diet: Role of estrogen receptor α and cytokines
Publication date: Available online 13 February 2020Source: Life SciencesAuthor(s): Zeinab Farhadi, Mohammad Khaksari, Hossein Azizian, Abbas Mortazaeizadeh, Mohammad Shabani, Nader Shahrokhi (Source: Life Sciences)
Source: Life Sciences - February 14, 2020 Category: Biology Source Type: research

Evodiamine inhibits proliferation and promotes apoptosis of hepatocellular carcinoma cells via the Hippo-Yes-Associated Protein signaling pathway
Publication date: Available online 11 February 2020Source: Life SciencesAuthor(s): Shuang Zhao, Ke Xu, Rong Jiang, Dan-Yang Li, Xing-Xian Guo, Peng Zhou, Jia-Feng Tang, Li-Sha Li, Di Zeng, Ling Hu, Jian-Hua Ran, Jing Li, Di-Long ChenAbstractAimsDysfunction of the Hippo-Yes-Associated Protein (YAP) signaling pathway is known to be associated with hepatocellular carcinoma (HCC). Evodiamine (Evo), a plant-derived bioactive alkaloid, exerts inhibitory effects on cancer. However, the precise influence of Evo on HCC and its potential effects on Hippo-YAP signaling have yet to be ascertained. Here, the effects of Evo on cell prol...
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research

Decreasing Arl4c expression by inhibition of AKT signal in human lung adenocarcinoma cells
ConclusionHere, we highlighted the AKT pathway as an important regulatory pathway for Arl4c expression in lung cancer cells and identified HCPT as a promising drug for lung adenocarcinoma treatment that functioned by targeting Arl4c expression. (Source: Life Sciences)
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research

Nicorandil ameliorates bleomycin-induced pulmonary fibrosis in rats through modulating eNOS, iNOS, TXNIP and HIF-1α levels
In conclusion, these results illustrate the possible potential effects of nicorandil for managing pulmonary fibrosis caused by BLM. (Source: Life Sciences)
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research

Amentoflavone suppresses cell proliferation and induces cell death through triggering autophagy-dependent ferroptosis in human glioma
ConclusionThese results demonstrate that AF triggered ferroptosis in autophagy-dependent manner. Our results suggest that AF has the potential to be considered as a novel treatment agent in glioma. (Source: Life Sciences)
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research

Resveratrol promotes osteogenesis via activating SIRT1/FoxO1 pathway in osteoporosis mice
This study aimed to investigate the effects of resveratrol (3, 4′, 5-trihydroxystilbene, RES) on osteoporosis and the role of SIRT1/FoxO1 pathway in the process.Main methodsIn vivo, mice were divided into 3 groups, Sham, ovariectomized (OVX) and OVX-RES group. Micro-CT, histology and histomorphometry were conducted to detect details of bone mass and microstructure. The expression of osteoblast markers was tested by Real-time qPCR and serum markers which reflected bone formation and resorption were analyzed by enzyme-linked immunosorbent assay (ELISA). Besides, we assayed sirtuin 1 (SIRT1) expression and the concentra...
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research

The regulation of TFEB in lipid homeostasis of non-alcoholic fatty liver disease: Molecular mechanism and promising therapeutic targets
Publication date: Available online 10 February 2020Source: Life SciencesAuthor(s): Shenglan Yu, Zhengtao Wang, Lili Ding, Li YangAbstractNonalcoholic fatty liver disease (NAFLD), which is characterized by disruption of lipid homeostasis, has been the leading cause of chronic liver disease worldwide. However, currently there is no effective therapy for NAFLD. Consequently, it is extremely urgent to explore the specific and effective target functioned as lipids regulator during the pathological process of NAFLD for the drug development. Transcription factor EB (TFEB) plays a crucial role in the regulation of lipid homeostasi...
Source: Life Sciences - February 11, 2020 Category: Biology Source Type: research

Immunomodulatory benefits of mesenchymal stem cells treated with Caffeine in adjuvant-induced arthritis
ConclusionIn general, caffeine-treated MSCs may be a promising strategy for cell-based therapy of RA.Graphical abstract (Source: Life Sciences)
Source: Life Sciences - February 11, 2020 Category: Biology Source Type: research

Inhibition of RELM-β prevents hypoxia-induced overproliferation of human pulmonary artery smooth muscle cells by reversing PLC-mediated KCNK3 decline
Publication date: Available online 8 February 2020Source: Life SciencesAuthor(s): Linlin Han, Nannan Song, Xiaomin Hu, Afang Zhu, Xin Wei, Jinmin Liu, Shiying Yuan, Weike Mao, Xiangdong ChenAbstractAimsAlthough resistin-like molecule β (RELM-β) is involved in the pathological processes of various lung diseases, such as pulmonary inflammation, asthma and fibrosis, its potential roles in hypoxic pulmonary arterial hypertension (PAH) remain largely unknown. The study aims to investigate whether RELM-β contributes to hypoxia-induced excessive proliferation of human pulmonary artery smooth muscle cells (PASMCs) a...
Source: Life Sciences - February 10, 2020 Category: Biology Source Type: research

Cell-free miRNAs as non-invasive biomarkers in breast cancer: Significance in early diagnosis and metastasis prediction
Publication date: Available online 7 February 2020Source: Life SciencesAuthor(s): Dharambir Kashyap, Harmandeep KaurAbstractBreast cancer is one of the genetic diseases causing a high mortality among women around the world. Despite the availability of advanced diagnostic tools and treatment strategies, the incidence of breast cancer is increasing every year. This is due to the lack of accurate and reliable biomarkers whose deficiency creates difficulty in early breast cancer recognition, subtypes determination, and metastasis prophecy. Although biomarkers such as ER, PR, Her2, Ki-67, and other genetic platforms e.g. MammaP...
Source: Life Sciences - February 8, 2020 Category: Biology Source Type: research

High-mobility group box 1 protein participates in acute lung injury by activating protein kinase R and inducing M1 polarization
Publication date: Available online 6 February 2020Source: Life SciencesAuthor(s): Ruiting Li, You Shang, Yuan Yu, Ting Zhou, Wei Xiong, Xiaojing ZouAbstractHigh-mobility group box 1 protein (HMGB1) is a crucial proinflammatory cytokine that contributes to acute lung injury (ALI). Macrophages are known to express the primary receptors (receptor for advanced glycation end products [RAGE], Toll-like receptor [TLR] 2, and TLR4) of HMGB1 for transmitting intracellular signals. Studies have revealed that double-stranded RNA activated protein kinase R (PKR), which is expressed in macrophages, participates in ALI by regulating mac...
Source: Life Sciences - February 7, 2020 Category: Biology Source Type: research

Investigation of endoplasmic reticulum stress and sonic hedgehog pathway in diabetic liver injury in mice
In conclusion, the Shh pathway is related to ER stress and may provide a new strategy for its treatment, especially liver stress induced by diabetes. (Source: Life Sciences)
Source: Life Sciences - February 7, 2020 Category: Biology Source Type: research