Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats
Publication date: Available online 12 November 2018Source: Life SciencesAuthor(s): Bo Shi, Shixing Li, Hao Ju, Xin Liu, Dan Li, Ying LiAbstractThe present study aimed to evaluate the renoprotective effects of chelerythrine (CHE), a protein kinase C inhibitor, on neonatal rats after partial unilateral ureteral obstruction (UUO) surgery. New born Sprague Dawley rats were subjected to partial UUO 48 h after birth and received a daily intraperitoneal injection of 5 mg/kg CHE. At 21-day age, the rats were scarified and the kidneys were collected for analysis. Results showed that CHE treatment significantly increased kidney ...
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

FTY720P inhibits the Na+/K+ ATPase in Caco-2 cells via S1PR2: PGE2 and NO are along the signaling pathway
Publication date: Available online 12 November 2018Source: Life SciencesAuthor(s): Reem Rida, Sawsan KreydiyyehAbstractAimsSphingosine-1-phosphate (S1P) has been implicated lately in inflammatory bowel disease which has diarrhea as one of its symptoms. Diarrhea is due to altered water movements as a result of altered electrolyte transport, and in particular sodium. Sodium movements are geared by the sodium gradient established by the Na+/K+ ATPase. The aim of this work was to investigate if S1P can modulate the activity of the ATPase, using Caco-2 cells as a model and the S1P analogue, FTY720P.Materials and methodsThe acti...
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

IGF-1 and somatocrinin trigger islet differentiation in human amniotic membrane derived mesenchymal stem cells
Publication date: Available online 13 November 2018Source: Life SciencesAuthor(s): Avinash Kharat, Bhawna Chandravanshi, Shashikant Gadre, Vikrant Patil, Ramesh Bhonde, Aparna DubhashiAbstractAimTo induce differentiation of human amniotic membrane derived mesenchymal stem cells (hAMMSCs) into insulin producing cells (IPCs) by treating with somatocrinin or growth hormone releasing hormone (GHRH) and Insulin-like growth factor-1 (IGF-1).Main methodIn this investigation, we cultivated and characterized hAMMSCs and then treated with IGF-1 and somatocrinin to find out whether this combination gives better yield of insulin produ...
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

Protective efficacy of crocetin and its nanoformulation against cyclosporine A-mediated toxicity in human embryonic kidney cells
This study is aimed to formulate crocetin-loaded lipid Nanoparticles (NPs) and to evaluate its antioxidant properties in a cyclosporine A-mediated toxicity in Human Embryonic Kidney (HEK-293) cells in vitro.Main methodsCrocetin-loaded NPs were prepared followed by physicochemical characterization. In vitro protective efficacy of crocetin and crocetin loaded NPs was investigated in cyclosporine A-mediated toxicity in HEK-293 cells by assessing free radical scavenging, DNA Nicking, cytotoxicity, intracellular Reactive oxygen species (ROS) inhibition, Mitochondrial membrane potential (MMPs) loss and evaluating the activity an...
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

Involvement of inducible nitric oxide synthase and estrogen receptor ESR2 (ERβ) in the vascular dysfunction in female type 1 diabetic rats
Publication date: Available online 14 November 2018Source: Life SciencesAuthor(s): Simone Marcieli Sartoretto, Fernanda Fernandes Santos, Beatriz Pereira Costa, Graziela Scalianti Ceravolo, Rosângela Santos-Eichler, Maria Helena Catelli Carvalho, Zuleica Bruno Fortes, Eliana Hiromi AkamineAbstractAimsInflammation is involved in diabetes-related vascular dysfunction. Estrogen receptor ESR2/ERβ induces the expression of inducible nitric oxide (NO) synthase (iNOS) and inflammation. The present study investigated the effect of alloxan-induced type 1 diabetes on the iNOS and ESR2 expression and the effect of the chro...
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

Diosmetin as a novel transient receptor potential vanilloid 1 antagonist with antinociceptive activity in mice
In conclusion, our results support the antinociceptive properties of diosmetin which seems to occur via TRPV1 antagonist in mice.Graphical abstract (Source: Life Sciences)
Source: Life Sciences - November 14, 2018 Category: Biology Source Type: research

Overexpression of Forkhead box C1 attenuates oxidative stress, inflammation and apoptosis in chronic obstructive pulmonary disease
Publication date: Available online 11 November 2018Source: Life SciencesAuthor(s): Shuyue Xia, Jian Qu, Hui Jia, Wei He, Jing Li, Long Zhao, Mingqing Mao, Yan ZhaoAbstractAimChronic obstructive pulmonary disease (COPD) is a disease caused by cigarette smoke, which has been emerging as a serious health problem worldwide. The aim of this study is to explore the mRNA expression profile of lung tissues from the COPD rats and to characterize the role of Forkhead box C1 (Foxc1) in COPD.Main methodsWistar rats were exposed to cigarette smoke during 16 weeks for COPD model establishment. The microarray was used to identify the d...
Source: Life Sciences - November 12, 2018 Category: Biology Source Type: research

Induction of functional erythropoietin and erythropoietin receptor gene expression by gamma-aminobutyric acid and piperine in kidney epithelial cells
Publication date: Available online 12 November 2018Source: Life SciencesAuthor(s): Yoon-Mi Lee, Jun-Ha Choi, Wan-Kwon Min, Jong-Kwon Han, Jae-Wook OhAbstractAimsThe aim of this study was to evaluate gamma-aminobutyric acid (GABA)- and piperine-induced erythropoietin (EPO) and EPO-receptor expression.Materials and methodsThe effect of GABA and piperine on cell viability was examined using kidney epithelial cells. Expression levels of EPO and EPO-R mRNA and protein were evaluated in response to GABA and piperine treatments. GABA- and piperine-mediated activation of the mitogen-activated protein kinase (MAPK) signaling pathwa...
Source: Life Sciences - November 12, 2018 Category: Biology Source Type: research

MicroRNA-499-5p regulates skeletal myofiber specification via NFATc1/MEF2C pathway and Thrap1/MEF2C axis
This study aimed to investigate the role of microRNA-499-5p (miR-499-5p) in the regulation of skeletal myofiber specification and its underlying mechanisms.Main methodsMouse C2C12 cells were used in this study. Cyclosporin A and siRNA targeting Thrap1 (si-Thrap1) were used to inhibit NFATc1/MEF2C pathway and knockdown Thrap1, respectively. The expressions of miR-499-5p and genes were evaluated by real-time quantitative PCR and western blot analysis.Key findingsOverexpression of miR-499-5p promoted oxidative fiber gene expression and repressed glycolytic fiber gene expression, affecting several factors associated with fiber...
Source: Life Sciences - November 11, 2018 Category: Biology Source Type: research

Cardioprotective and anti-apoptotic effects of Potentilla reptans L. root via Nrf2 pathway in an isolated rat heart ischemia/reperfusion model
ConclusionsP. reptans demonstrated that the cardioprotective preconditioning effects via NO release, Nrf2 pathway, and antioxidant activity lead to a decrease in the apoptotic index. (Source: Life Sciences)
Source: Life Sciences - November 11, 2018 Category: Biology Source Type: research

A meta-analysis of dysregulated miRNAs in coronary heart disease
Publication date: Available online 10 November 2018Source: Life SciencesAuthor(s): Si-Si Wang, Li-Juan Wu, Jia-Jiang-Hui Li, Huan-Bo Xiao, Yan He, Yu-Xiang YanAbstractAimsTo combine the results of dysregulated miRNAs in individual coronary heart disease (CHD) studies and to identify potential miRNA biomarkers.Main methodsMiRNA profiling studies of CHD were extracted from Pubmed, Embase, Web of Science and China National Knowledge Internet (CNKI) databases if they met the inclusion criteria. The meta-analysis was conducted using a random effects model to identify the effect of each multiple-reported miRNA. We also performed...
Source: Life Sciences - November 11, 2018 Category: Biology Source Type: research

Protective effects of ginsenoside Rg3 on TNF-α-induced human nucleus pulposus cells through inhibiting NF-κB signaling pathway
Publication date: Available online 11 November 2018Source: Life SciencesAuthor(s): Jiang Chen, Gen-Zhe Liu, Qi Sun, Fan Zhang, Chu-yin Liu, Lin Yuan, Xue-Qian Zhao, Yong-Jun Wang, Yu-Song JiaAbstractThis work aims to evaluate the effect of ginsenoside Rg3 on the apoptosis, proliferation, extracellular matrix (ECM) metabolism and oxidative stress-induced damage of human nucleus pulposus cells (NPCs) induced by TNF-α. The human NPCs were divided into Control, TNF-α, TNF-α + low Rg3, TNF-α + medium Rg3 and TNF-α + high Rg3 groups. Annexin V-FITC/PI, CCK-8 and flow cytometry were used ...
Source: Life Sciences - November 11, 2018 Category: Biology Source Type: research

ERK and miRNA-1 target Cx43 expression and phosphorylation to modulate the vascular protective effect of angiotensin II
In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and microRNA (miR-1) in this process.Key findingsOur results show that after hemorrhagic shock or hypoxia, the blockade of GJs or knockdown of Cx43 inhibits the AngII-induced increase in vascular reactivity of superior mesenteric arteries and the contractile response of vascular smooth muscle cells (VSMCs). AngII treatment increases Cx43 expression and phosphorylation at Ser262, and restores gap-junctional communication (GJIC) between VSMCs afte...
Source: Life Sciences - November 9, 2018 Category: Biology Source Type: research

Integrative proteomic analysis reveals potential high-frequency alternative open reading frame-encoded peptides in human colorectal cancer
Publication date: Available online 9 November 2018Source: Life SciencesAuthor(s): Tianfang Wang, Yining Liu, Qi Liu, Scott Cummins, Min ZhaoAbstractIdentification of alternative open reading frame-encoded peptides (AEPs) for the diagnosis of colorectal cancer at the proteome level is largely unexplored because of a lack of comprehensive proteomics data. Here, we performed a comprehensive integrative analysis of mass spectral data published by Clinical Proteomic Tumor Analysis Consortium and characterized 93 high-confident AEPs encoded within 75 genes. There are four cancer-related genes appeared to have AEPs identified fre...
Source: Life Sciences - November 9, 2018 Category: Biology Source Type: research

Antinociceptive and anti-inflammatory effect of Naringenin in different nociceptive and inflammatory mice models
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Na Xue, Xianwei Wu, Li Wu, Lu Li, Fang WangAbstractInflammation is the vital defensive response triggered by our immune system against an infection or deleterious stimuli. This response in due course affects its own biological system leading to serious diseases like arteriosclerosis, osteoporosis, pancreatitis, cancer etc. Currently, researchers focused on utilizing phytochemicals as anti-inflammatory drugs since the drugs presently available in the market causes serious side effects and are less potent. Flavonoids are polyphenols which impar...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Loss of pigment epithelium-derived factor leads to ovarian oxidative damage accompanied by diminished ovarian reserve in mice
This study aims to investigate the pathophysiological role and mechanism of pigment epithelium-derived factor (PEDF) deletion in ovarian damage.MethodsFemale PEDF-knockout mice and their wild-type littermates were used in this study. Relevant tests were performed at 8–10 weeks or 32 weeks of age.Key findingsCompared to the wild-type mice, the PEDF-knockout mice showed diminished ovarian reserve (DOR), worse ovum quality after injection to induce controlled ovarian stimulation, increased serum follicle stimulating hormone (FSH) level and an follicle stimulating hormone/luteinizing hormone (FSH/LH) ratio. Moreover,...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Trigonelline therapy confers neuroprotection by reduced glutathione mediated myeloperoxidase expression in animal model of ischemic stroke
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Kanta Pravalika, Deepaneeta Sarmah, Harpreet Kaur, Kanchan Vats, Jackson Saraf, Madhuri Wanve, Kiran Kalia, Anupom Borah, Dileep R. Yavagal, Kunjan R. Dave, Pallab BhattacharyaAbstractAimStroke is devastating with a limited choice of intervention. Many pharmacological entities are available but none of them have evolved successfully in counteracting the multifaceted molecular alterations following stroke. Myeloperoxidase (MPO) has been reported to play an important role in neuroinflammation following neurodegenerative diseases. Therefore, usi...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Energy and metabolic pathways in trefoil factor family member 2 (Tff2) KO mice beyond the protection from high-fat diet-induced obesity
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Abdelaziz Ghanemi, Aicha Melouane, Octave Mucunguzi, Mayumi Yoshioka, Jonny St-AmandAbstractAimsTrefoil factor family member 2 (TFF2) is a small gut peptide. We have previously shown that Tff2 knock out (KO) mice are protected from high-fat (HF) diet-induced obesity (De Giorgio et al., 2013a). Thus, exploring Tff2 KO-related pathways of mice at the genomic, proteinic and biochemical levels would allow us to elucidate the processes behind this protection from obesity.Main methodsTo explore the metabolic and energetic effects related to Tff2 de...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Metformin alleviates hyperglycemia-induced apoptosis and differentiation suppression in osteoblasts through inhibiting the TLR4 signaling pathway
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Lifeng Zheng, Ximei Shen, Junjian Ye, Yun Xie, Sunjie YanAbstractAimsMetformin was found to protect against hyperglycemia-induced injury in osteoblasts, but the cellular mechanisms involved remain unclear. Therefore, the aim of this study was to determine the effect of metformin on hyperglycemia-induced apoptosis and differentiation suppression in osteoblasts and to explore its relationships with the TLR4 signaling pathway.Main methodsA mouse osteoblast cell line, MC3T3-E1, and a diabetic rat model were used to survey the protective effects o...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats
Publication date: Available online 8 November 2018Source: Life SciencesAuthor(s): M.A. Avila-Ramírez, R.L. Esteban-Martínez, E. López-Moctezuma, L. Anguiano-Robledo, M.E. Hernández-Campos, P. López-SánchezAbstractAimsPregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illness...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Monomethyl fumarate alleviates sepsis-induced hepatic dysfunction by regulating TLR-4/NF-κB signalling pathway
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Armin Azadkhah Shalmani, Mohammad Hossein Ghahremani, Fereshteh Jeivad, Amir Shadboorestan, Gholamreza Hassanzadeh, Abbas Beh-Pajooh, Mikhriy Ganbari-Erdi, Sara Kasirzadeh, Mojtaba Mojtahedzadeh, Omid SabzevariAbstractAimsSepsis is a potentially fatal illness that can lead to impairment of multiple organs such as liver. The condition is deeply associated with oxidative stress and inflammation. Monomethyl fumarate (MMF) has manifested antioxidant and immunomodulatory properties. The aim of current study was to evaluate protective effects of MM...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Polymeric nanoparticles promote endocytosis of a survivin molecular beacon: Localization and fate of nanoparticles and beacon in human A549 cells
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Barbara Adinolfi, Mario Pellegrino, Sara Tombelli, Cosimo Trono, Ambra Giannetti, Claudio Domenici, Greta Varchi, Giovanna Sotgiu, Marco Ballestri, Francesco BaldiniAbstractPolymethylmethacrylate core-shell fluorescent nanoparticles promote, in human lung A549 cancer cells, the internalization of a molecular beacon (MB) specific for survivin mRNA, an anti-apoptotic protein overexpressed in cancer cells.AimsTo design an effective drug delivery system, the knowledge of the uptake mechanism and of the nanoparticles (NPs) and MB fate is required....
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Intestinal luminal content from high-fat-fed prediabetic mice changes epithelial barrier function in vitro
In this study, we investigated the effect of the intestinal luminal content isolated from control or HFD-fed prediabetic mice upon the tight junction (TJ)-mediated epithelial barrier in Caco-2 and MDCK epithelial cell lines.Methods/key findingsExposure to small intestine luminal content (SI) isolated from HFD-fed prediabetic mice induced a more significant decrease in transepithelial electrical resistance (TEER), associated with higher paracellular flux in Caco-2 and MDCK cells after 6 h and 4 h respectively, as compared to the SI obtained from control mice. Such changes were accompanied by a significant decrease in TJ...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Differential effects of maternal high-fat/high-caloric or isocaloric diet on offspring's skeletal muscle phenotype
This study sought to investigate the effects of two different maternal high-fat diets, during gestation and lactation, on the morphology of the skeletal muscle of the adult offspring rats.MethodsFemale Wistar rats were fed Control (C) or High-fat/high-caloric (HH) or High-fat/isocaloric (HI) diet during gestation and lactation. The somatic growth of the offspring was measured throughout lactation. Glucose and insulin tolerance tests were performed at PND61 and PND65, respectively. At PND70, soleus and extensor digitorum longus (EDL) muscles were removed for myofibrillar ATPase staining analysis.Key findingsHH pups were hea...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Contribution of sulfate-reducing bacteria to homeostasis disruption during intestinal inflammation
Publication date: Available online 7 November 2018Source: Life SciencesAuthor(s): Vanessa Ribeiro Figliuolo, Robson Coutinho-Silva, Claudia Mara Lara Melo CoutinhoAbstractAlteration in microbial populations and metabolism are key events associated with disruption of intestinal homeostasis and immune tolerance during intestinal inflammation. A substantial imbalance in bacterial populations in the intestine and their relationships with the host have been observed in patients with inflammatory bowel disease (IBD), believed to be part of an intricate mechanism of triggering and progression of intestinal inflammation. Because e...
Source: Life Sciences - November 8, 2018 Category: Biology Source Type: research

Acyclovir induces cell cycle perturbation and apoptosis in Jurkat leukemia cells, and enhances chemotherapeutic drug cytotoxicity
Publication date: Available online 4 November 2018Source: Life SciencesAuthor(s): Serena Benedetti, Simona Catalani, Francesco Palma, Barbara Canonico, Francesca Luchetti, Rossella Galati, Stefano Papa, Serafina BattistelliAbstractAims and methodsMany antiviral agents have been reported to present direct cytotoxic activity in cancer, showing antiproliferative and proapoptotic effects through different mechanisms. In the present study, we took into account the cytotoxic action of the antiviral drug acyclovir (ACV) on leukemia cells, by investigating cell cycle perturbations and apoptosis induction upon drug administration t...
Source: Life Sciences - November 6, 2018 Category: Biology Source Type: research

CB2-deficiency is associated with a stronger hypertrophy and remodeling of the right ventricle in a murine model of left pulmonary artery occlusion
Publication date: Available online 4 November 2018Source: Life SciencesAuthor(s): Georg Daniel Duerr, Andreas Feißt, Katharina Halbach, Luise Verfuerth, Christopher Gestrich, Daniela Wenzel, Andreas Zimmer, Johannes Breuer, Oliver DewaldAbstractAimsPulmonary hypertension (PH) leads to right ventricular (RV) adaptation and remodeling and has deleterious long-term effects on RV function. The endocannabinoid receptor CB2 has been associated with protective effects in adaptation and remodeling of the left ventricle after ischemia. Therefore, we investigated the role of CB2 receptor in RV adaptation after occlusion of the...
Source: Life Sciences - November 6, 2018 Category: Biology Source Type: research

Tracking acetate through a journey of living world: Evolution as alternative cellular fuel with potential for application in cancer therapeutics
Publication date: Available online 5 November 2018Source: Life SciencesAuthor(s): Shrish Kumar Pandey, Saveg Yadav, Mithlesh Kumar Temre, Sukh Mahendra SinghAbstractAcetate is a short chain fatty acid, comprising carbon, hydrogen and oxygen (C2H3O2−) has emerged as a key alternative fuel for cellular metabolism. Beginning this voyage from the abiotic atmosphere, acetate has contributed to the physiology of both prokaryotes and eukaryotes. The main role of acetate includes its contribution to the global carbon cycle, bioenergetic and biosynthetic metabolic processes. Based on the ability to produce and consume organis...
Source: Life Sciences - November 6, 2018 Category: Biology Source Type: research

Development and characterization of late-stage diabetes mellitus and -associated vascular complications
Publication date: Available online 6 November 2018Source: Life SciencesAuthor(s): Gunjan Sharma, Md. Umama Ashhar, Vidhu Aeri, Deepshikha Pande KatareAbstractPreclinical investigation is the key mark of medical research, as the major breakthroughs including treatment of devastating diseases in biomedical research have been led by animal studies. Type 2 diabetes mellitus (T2DM) is a predominant metabolic disorder having high prevalence of morbidity worldwide which create an urgent need to understand the pathogenesis, complication and other possible influences by development of appropriate animal model. High-fat diet (HFD) f...
Source: Life Sciences - November 6, 2018 Category: Biology Source Type: research

Assessment of PKA and PKC inhibitors on force and kinetics of non-failing and failing human myocardium
Publication date: Available online 3 November 2018Source: Life SciencesAuthor(s): Nancy S. Saad, Mohammad T. Elnakish, Elizabeth A. Brundage, Brandon J. Biesiadecki, Ahmet Kilic, Amany A.E. Ahmed, Peter J. Mohler, Paul M.L. JanssenAbstractAimsHeart failure (HF) is a prevalent disease that is considered the foremost reason for hospitalization in the United States. Most protein kinases (PK) are activated in heart disease and their inhibition has been shown to improve cardiac function in both animal and human studies. However, little is known about the direct impact of PKA and PKC inhibitors on human cardiac contractile funct...
Source: Life Sciences - November 4, 2018 Category: Biology Source Type: research

Paracrine effect of mesenchymal stem cell as a novel therapeutic strategy for diabetic nephropathy
Publication date: Available online 3 November 2018Source: Life SciencesAuthor(s): Hongde Li, Pengfei Rong, Xiaoqian Ma, Wei Nie, Cheng Chen, Cejun Yang, Juan Zhang, Qiong Dong, Wei WangAbstractDiabetic nephropathy (DN) is a microvascular complication of diabetes mellitus (DM) and the main reason for end-stage renal diseases (ESRD). Based on the role of mesenchymal stem cells (MSCs) in regenerative medicine, the MSC therapy has been considered a promising strategy to ameliorate the progression of DN. In this article, we review the therapeutic potential of MSCs in DN, mainly involving MSC paracrine mechanism based on trophic...
Source: Life Sciences - November 4, 2018 Category: Biology Source Type: research

Knockdown of NRSF inhibits cell proliferation of ovarian cancer via activating Hippo pathway
Publication date: Available online 2 November 2018Source: Life SciencesAuthor(s): Pengfei Deng, Yan Zuo, Shuxian Feng, Zongtao Li, Wan Chen, Huawen Li, Xiaoyu WangAbstractAimsOvarian cancer is the most leading cause of deaths among gynecologic malignancies, and Neuron-restrictive silencer factor (NRSF) can be upregulated or downregulated according to the type of tumor. However, the expression and function of NRSF in ovarian cancer is still unknown.Main methodsExpression of NRSF in normal ovary and ovarian cancer cells were evaluated by quantitative PCR (qPCR). NRSF expression in normal ovary and ovarian cancer tissue sampl...
Source: Life Sciences - November 3, 2018 Category: Biology Source Type: research

Alogliptin abates memory injuries of hepatic encephalopathy induced by acute paracetamol intoxication via switching-off autophagy-related apoptosis
Publication date: Available online 1 November 2018Source: Life SciencesAuthor(s): Muhammed A. Saad, Alyasaa A. Rastanawi, Muhammed F. El-YamanyAbstractAimsHepatic encephalopathy (HE) is a complex neuropsychiatric syndrome. Paracetamol (APAP) causes, in high doses, a hepatic injury. Alogliptin (ALO), with its 100% oral bioavailability, may be able to reverse the acute hepatic injury and memory impairments.Materials and methodsForty rats were divided into four groups as follows; Normal Control Group, APAP intoxicated group, ALO and SIL groups. Behavioral tests (Morris water maze, Y-maze spontaneous alteration, and novel obje...
Source: Life Sciences - November 2, 2018 Category: Biology Source Type: research

Carnosine improves functional recovery and structural regeneration after sciatic nerve crush injury in rats
Publication date: Available online 1 November 2018Source: Life SciencesAuthor(s): Navideh Mirzakhani, Amir Abbas Farshid, Esmaeal Tamaddonfard, Mehdi Imani, Amir Erfanparast, Farahnaz NorooziniaAbstractAimsPeripheral nerve injury represents a substantial clinical problem with insufficient or unsatisfactory treatment options. Current researches have extensively focused on the new approaches for the treatment of peripheral nerve injuries. Carnosine is a naturally occurring pleotropic dipeptide and has many biological functions such as antioxidant property. In the present study, we examined the regenerative ability of carnosi...
Source: Life Sciences - November 2, 2018 Category: Biology Source Type: research

Pharmacokinetic, pharmacodynamic and formulations aspects of Naringenin: An update
Publication date: Available online 1 November 2018Source: Life SciencesAuthor(s): Ruthvika Joshi, Yogesh A. Kulkarni, Sarika WairkarAbstractPhenolic compounds constitute one of the important classes of secondary metabolites in the plants. Flavonoids are primary phenolic compounds found in natural drugs. Naringenin is a flavanone, aglycone of Naringin, predominantly found in citrus fruits with various pharmacological activities. Large number of scientific papers has been published on Naringenin describing its structure, physicochemical properties and its therapeutic use in different diseases. This review provides highlights...
Source: Life Sciences - November 2, 2018 Category: Biology Source Type: research

HIV-1 Nef-POTEE; A novel interaction modulates macrophage dissemination via mTORC2 signaling pathway
Publication date: Available online 31 October 2018Source: Life SciencesAuthor(s): Umeshkumar Vekariya, Reshu Saxena, Poonam Singh, Kavita Rawat, Balawant Kumar, Sushila Kumari, Saurabh Kumar Agnihotri, Supinder Kaur, Rekha Sachan, Aamir Nazir, Smrati Bhadauria, Monika Sachdev, Raj Kamal TripathiAbstractAimsHuman immunodeficiency virus −1 [HIV-1] Nef, localizes in different cellular compartments and modulates several cellular pathways. Nef promotes virus pathogenicity through alteration in cell surface receptor expression, apoptosis, protein trafficking etc. Nef regulates viral pathogenesis through interaction with di...
Source: Life Sciences - November 1, 2018 Category: Biology Source Type: research

Elevated expression of Twinfilin-1 is correlated with inferior prognosis of lung adenocarcinoma
Publication date: Available online 31 October 2018Source: Life SciencesAuthor(s): Zhang Kaishang, Pan Xue, Zheng Shaozhong, Fan Yingying, Zhang Yan, Sun Chanjun, Li Zhenzhen, Li XiangnanAbstractAimTwinfilin-1 (TWF1) has been implicated in cell motility, invasion and migration. However, its exact role in lung cancer progression is still unclear. In the present study, we explored clinical and prognostic relevance of Twinfilin-1 (TWF1) levels for non-small cell lung carcinoma (NSCLC).Main methodsThe Cancer Genome Atlas (TCGA) dataset was analyzed for possible association between TWF1 expressions in NSCLC tissues and patient p...
Source: Life Sciences - November 1, 2018 Category: Biology Source Type: research

Protective autophagy induced by physcion suppresses hepatocellular carcinoma cell metastasis by inactivating the JAK2/STAT3 Axis
Publication date: Available online 30 October 2018Source: Life SciencesAuthor(s): Xiaoping Pan, Chen Wang, Yan Li, Lida Zhu, Ti ZhangAbstractAimAlthough the anti-neoplastic effects of physcion are well documented, its specific action in hepatocellular carcinoma (HCC) is not understood. Taken together, physcion is a promising drug candidate for the treatment and prevention of HCC.Material and methodsCell Counting Kit-8 (CCK-8) assay utilized to examine the viability of Hep3B and SMMC7721 cells. Apoptotic cell population was measured by flow cytometry analysis assay. Cell migration and cell invasion were determined by wound ...
Source: Life Sciences - October 31, 2018 Category: Biology Source Type: research

Emerging roles of YAP/TAZ in lung physiology and diseases
Publication date: Available online 30 October 2018Source: Life SciencesAuthor(s): Haojun Xie, Liquan Wu, Zhenan Deng, Yating Huo, Yuanxiong ChengAbstractThe YAP and TAZ, as the downstream effectors of Hippo pathway, have emerged as important translational co-activators of a wide variety of biological processes. YAP/TAZ plays a crucial role in the lung development and physiology. Dysregulation of YAP/TAZ signaling pathway contributes to the development and progression of chronic lung diseases, including lung cancer, pulmonary fibrosis, pulmonary hypertension, COPD, asthma, and lung infection. Therefore, owing to its critica...
Source: Life Sciences - October 30, 2018 Category: Biology Source Type: research

Alpha-lipoic acid and coenzyme Q10 combination ameliorates experimental diabetic neuropathy by modulating oxidative stress and apoptosis
Publication date: Available online 28 October 2018Source: Life SciencesAuthor(s): Nasrin Sadeghiyan Galeshkalami, Mohammad Abdollahi, Rezvan Najafi, Maryam Baeeri, Akram Jamshidzade, Reza Falak, Mohammad Davoodzadeh Gholami, Gholamreza Hassanzadeh, Tahmineh Mokhtari, Shokoufeh Hassani, Mahban Rahimifard, Asieh HosseiniAbstractAimsDiabetic neuropathy (DN) is the most common complication of diabetes. Neuroprotective effects of alpha lipoic acid (ALA) and coenzyme Q10 (CoQ10) has been previously shown in DN, but underlying mechanisms involved not been exactly found. The present study explored the neuroprotective effects of AL...
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Homocysteine accelerates atherosclerosis via inhibiting LXRα–mediated ABCA1/ABCG1–dependent cholesterol efflux from macrophages
Publication date: Available online 28 October 2018Source: Life SciencesAuthor(s): Ping Jin, Yitong Bian, Kai Wang, Guangzhi Cong, Ru Yan, Yong Sha, Xueping Ma, Juan Zhou, Zuyi Yuan, Shaobin JiaAbstractAimsMacrophage-derived foam-cell formation plays a crucial role in the development of atherosclerosis, and liver X receptor alpha (LXRα) is a key regulator of lipid metabolism in macrophages. Homocysteine (Hcy) is an independent risk factor of atherosclerosis; however, the regulation of lipid metabolism and role of LXRα induced by Hcy in macrophages is still unknown. The present study aimed to investigate the pote...
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Tissue regeneration: Impact of sleep on stem cell regenerative capacity
Publication date: Available online 28 October 2018Source: Life SciencesAuthor(s): Hoda Elkhenany, Abdelrahman AlOkda, Ahmed El-Badawy, Nagwa El-BadriAbstractThe circadian rhythm orchestrates many cellular functions, such as cell division, cell migration, metabolism and numerous intracellular biological processes. The physiological changes during sleep are believed to promote a suitable microenvironment for stem cells to proliferate, migrate and differentiate. These effects are mediated either directly by circadian clock genes or indirectly via hormones and cytokines. Hormones, such as melatonin and cortisol, are secreted i...
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Adipose-derived stem cells reduce liver oxidative stress and autophagy induced by ischemia-reperfusion and hepatectomy injury in swine
In conclusion, ADSCs attenuate hepatic I/R and hepatectomy-induced liver damage by reducing oxidative stress and inhibiting autophagy. (Source: Life Sciences)
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Interaction between gut microbiome and cardiovascular disease
Publication date: Available online 29 October 2018Source: Life SciencesAuthor(s): Jieting Peng, Xun Xiao, Min Hu, Xiangyu ZhangAbstractTraditional cardiovascular risk factors do not underlie all incidence of cardiovascular disease. In recent years, accumulating evidence has demonstrated that gut microbiota and its metabolites also play a pivotal role in the onset and development of cardiovascular disease, including atherosclerosis, hypertension, heart failure, atrial fibrillation and myocardial fibrosis. Trillions of bacteria indwell the gastrointestinal tract and metabolize nutrients into trimethylamine-N-oxide, short-cha...
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Editorial Board
Publication date: 15 November 2018Source: Life Sciences, Volume 213Author(s): (Source: Life Sciences)
Source: Life Sciences - October 29, 2018 Category: Biology Source Type: research

Matrikines for therapeutic and biomedical applications
Publication date: Available online 26 October 2018Source: Life SciencesAuthor(s): K. Sivaraman, C. ShanthiAbstractMatrikines, peptides originating from the fragmentation of extracellular matrix proteins are identified to play important role in both health and disease. They possess biological activities, much different from their parent protein. Identification of such bioactive cryptic regions in the extracellular matrix proteins has attracted the researchers all over the world in the recent decade. These bioactive peptides could find use in preparation of biomaterials and tissue engineering applications. Matrikines identif...
Source: Life Sciences - October 27, 2018 Category: Biology Source Type: research

p53 as a double-edged sword in the progression of non-alcoholic fatty liver disease
Publication date: Available online 26 October 2018Source: Life SciencesAuthor(s): Zhibin Yan, Xiaokang Miao, Bangzhi Zhang, Junqiu XieAbstractNon-alcoholic fatty liver disease (NAFLD) derives from the accumulation of hepatic lipids, which leads to liver steatosis and then triggers non-alcoholic steatohepatitis, sometimes worsening to hepatic fibrosis, cirrhosis, and hepatocellular carcinoma. Although the molecular mechanisms of NAFLD have been intensively investigated, its pathogenesis remains poorly understood and needs to be clarified. Tumor-suppressor factor p53 has a crucial role in many signaling pathways that induce ...
Source: Life Sciences - October 27, 2018 Category: Biology Source Type: research

ApoA-1 accelerates regeneration of small-for-size fatty liver graft after transplantation
ConclusionsApoA-1 may accelerate regeneration of small-for-size fatty liver graft at day 2 after transplantation through regulating mitochondrial function. ApoA-1 may be the potential new therapy of promoting liver regeneration. (Source: Life Sciences)
Source: Life Sciences - October 27, 2018 Category: Biology Source Type: research

CRTC2 suppresses BMP2-induced osteoblastic differentiation via Smurf1 expression in MC3T3-E1 cells
Publication date: Available online 26 October 2018Source: Life SciencesAuthor(s): Kyeong-Min Kim, Wan-Jin Jeon, Eun-Jung Kim, Won-Gu JangAbstractAimsCREB (cAMP response element-binding protein)-regulated transcription coactivator (CRTC2) has been reported to act as a coactivator of CREB during gluconeogenesis. The role of CRTC2 in osteoblastic differentiation has not yet been elucidated. The aim of this study is to identify the mechanism of CRTC2 in osteoblast differentiation.Main methodsThe mRNA expression was determined by RT-PCR and qPCR. Protein levels were measured using Western blot assay. Alkaline phosphatase (ALP) ...
Source: Life Sciences - October 27, 2018 Category: Biology Source Type: research

Testosterone mediates hyperthermic response of mice to heat exposure
We examined effects of testosterone on thermal response of mice to heat exposure.Main methodsAdult C57BL/6J male mice received gonadectomy or sham surgery subsequent vehicle (Gnx) or testosterone implants (Gnx+T). Body core temperature (Tc) of mice was recorded telemetrically during acute heat exposure. Thermal responses to heat exposure were also examined in age-matched female mice at each stage of the estrous cycle.Key findingsBasal Tc was lower in sham male mice than females, but did not differ among sham, Gnx or Gnx+T males. No alterations in expression of uncoupling proteins 2 and 3 in the gastrocnemius muscle were fo...
Source: Life Sciences - October 27, 2018 Category: Biology Source Type: research