Pharmacological profile of TAN-452, a novel peripherally acting opioid receptor antagonist for the treatment of opioid-induced bowel syndromes
The objective was to characterize the pharmacological profile of 17-cyclopropylmethyl-6,7-didehydro-4,5α-epoxy-6′-ethoxycarbonyl-3,14-dihydroxyindolo [2′,3′-6,7]morphinan (TAN-452), a novel peripherally acting opioid receptor antagonist.Main methodsThe in vitro binding affinity for the μ-, δ-, and κ-opioid receptors (MOR, DOR, and KOR) and the inhibition of [35S]GTPγS binding were examined using membrane preparations from recombinant human (h) MOR, DOR, or KOR expressing cell. In vivo assays were performed to determine the inhibitory effect of TAN-452 on morphine-induced emesis ...
Source: Life Sciences - July 21, 2018 Category: Biology Source Type: research

Long non-coding RNA HOTAIR promotes LPS-induced inflammatory injury by down-regulation of microRNA-124 in murine chondrogenic ATDC5 cells
Publication date: Available online 20 July 2018Source: Life SciencesAuthor(s): Guoqing Duan, Shiqiang Song, Shuaishuai NiuAbstractAimsLong non-coding RNAs (lncRNAs) exert important functions in pathogenesis of osteoarthritis (OA). Our study aimed to explore the effects of lncRNA HOX transcript antisense RNA (HOTAIR) on LPS-induced inflammatory injury in ATDC5 cells, as well as potential mechanism(s).Main methodsHOTAIR expression was measured in both OA patients and non-OA patients. ATDC5 cells were treated with LPS and/or transfected with pc-HOTAIR, sh-HOTAIR, miR-124 mimic and their corresponding negative controls. The ex...
Source: Life Sciences - July 21, 2018 Category: Biology Source Type: research

Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways
Publication date: Available online 20 July 2018Source: Life SciencesAuthor(s): Wei Qu, Zhenguo Zhao, Sijin Chen, Lijin Zhang, Danlian Wu, Zhigao ChenAbstractBisphenol A (BPA) can act as pathogenic agent on mammalian cells. However, whether BPA induces colonic epithelial cell damage remains unexplored. We used HCT116 cells as a model to investigate the negative effect of BPA on human colon cancer cells and explore the potential mechanism. Our results suggest that BPA decreased viability of HCT116 cells. BPA also caused serious oxidative damage to the colonic epithelium, as indicated by increased mitochondrial and intracellu...
Source: Life Sciences - July 21, 2018 Category: Biology Source Type: research

Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity
Publication date: Available online 20 July 2018Source: Life SciencesAuthor(s): Sergio M. Borghi, Victor Fattori, Kenji W. Ruiz-Miyazawa, Mariana M. Bertozzi, Yuri Lourenco-Gonzalez, Roberto I. Tatakihara, Allan J.C. Bussmann, Tânia L. Mazzuco, Rubia Casagrande, Waldiceu A. VerriAbstractAimsNon-steroidal anti-inflammatory drugs (NSAIDs) are widely used and effective anti-inflammatories despite the well-known side effects such as gastrointestinal damage, acute kidney injury (AKI), and cardiovascular dysfunctions. Diclofenac is among the most prescribed NSAIDs due to its efficient analgesic and anti-inflammatory propert...
Source: Life Sciences - July 21, 2018 Category: Biology Source Type: research

Exploring the potential of marine microbes in clinical management of Alzheimer's disease: A road map for bioprospecting and identifying promising isolates
Publication date: Available online 19 July 2018Source: Life SciencesAuthor(s): Anuraag Muralidharan, J. Venkata Rao, H. Raghu ChandrashekharAbstractPervasiveness of Alzheimer's disease (AD) across the globe is on rise, devitalizing the essential brain functions of the afflicted individual. Multiple neurological pathways viz., cholinergic, amyloidogenic and tau protein pathways underlying the disease and interdependence make it more complex to develop effective treatment strategies. Existing drug treatments for Alzheimer's disease majorly belong to the class of cholinergic inhibitors which improve the behavioral symptoms. B...
Source: Life Sciences - July 20, 2018 Category: Biology Source Type: research

TNNT1 facilitates proliferation of breast cancer cells by promoting G1/S phase transition
Publication date: Available online 19 July 2018Source: Life SciencesAuthor(s): Yawei Shi, Yang Zhao, Yunjian Zhang, NiJiati AiErken, Nan Shao, Runyi Ye, Ying Lin, Shenming WangAbstractAimsBreast cancer is the major diagnosed cancer and the leading reason of cancer related death among women, and the tumor size is one of the risk factors. Therefore, it is significant to reveal the principle of breaking the subtle homeostasis of cell cycle and sustaining chronic proliferation of cancer cells.Main methodsThe expression of TNNT1 was examined by qPCR and western blotting. The effect of TNNT1 on cell proliferation was detected by...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Resveratrol sensitizes lung cancer cell to TRAIL by p53 independent and suppression of Akt/NF-κB signaling
Publication date: Available online 19 July 2018Source: Life SciencesAuthor(s): Mohammad Rasheduzzaman, Jae-Kyo Jeong, Sang-Youel ParkAbstractAimsTRAIL is a promising anticancer agent that has the potential to sensitize a wide variety of cancer or transformed cells by inducing apoptosis. However, resistance to TRAIL is a growing concern. Current manuscript aimed to employing combination treatment to investigate resveratrol induced TRAIL sensitization in NSCLC.MethodA549 and HCC-15 cells were used in experimental design. Cell viability was determined by morphological image, crystal violet staining and MTT assay. Apoptosis wa...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Specific deletion connexin43 in astrocyte ameliorates cognitive dysfunction in APP/PS1 mice
Publication date: Available online 19 July 2018Source: Life SciencesAuthor(s): Ren Runan, Zhang Li, Wang MinAbstractEmerging data indicate an important role for connexin43 (Cx43) in cognitive function, but there is a lack of direct evidence of the role of astroglial Cx43 in cognitive dysfunction in Alzheimer's disease (AD). Here we evaluated the expression pattern of Cx43 in AD and found progressive upregulation of the mRNA and protein levels of Cx43. Subsequently, we generated an astroglial Cx43 knockout (KO) AD mouse model by crossbreeding Gfap (glial fibrillary acidic protein)-Cx43 KO mice with APP/PS1 mice. Then we ass...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

CENP-E as a target for cancer therapy: Where are we now?
Publication date: Available online 19 July 2018Source: Life SciencesAuthor(s): Amr Ahmed El-Arabey, Salama Abdu Salama, Adel Rashad Abd-AllahAbstractIn 2015, more than 1.6 million new cancer cases with 589,430 deaths were estimated over worldwide. Cancer is a complex disease with abnormal cell growth control which is hallmarked by chromosome misalignment and consequently genomic instability. Mitosis is a well-known target for chemotherapy as taxol and colchicines inhibit tumor cell division by inhibiting mitotic spindle plasticity. Accumulating evidence has revealed that the Centromere-associated protein E (CENP-E) is expr...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Plasma miR-17, miR-20a, miR-20b and miR-122 as potential biomarkers for diagnosis of NAFLD in type 2 diabetes mellitus patients
This study aimed to identify circulating miRNAs for early diagnosis of the complication.Materials and methodsPlasma miRNA expression profiles of T2DM patients complicated with or without NAFLD were examined by miRNA array analysis and then were validated by qRT-PCR. A new index for prediction the presence of NAFLD was developed based on the result of multivariate logistic regression analysis. STZ and high fat diet were used for construction a rat model of T2DM complicated with NAFLD.Key findingsPlasma miR-17, miR-20a, miR-20b, and miR-122 were up-regulated in T2DM patients with NAFLD complicated compared in those without NAFLD (P 
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Quercetin suppresses the mobility of breast cancer by suppressing glycolysis through Akt-mTOR pathway mediated autophagy induction
Publication date: Available online 17 July 2018Source: Life SciencesAuthor(s): Lijun Jia, Shan Huang, Xiaoran Yin, Ying Zan, Ya Guo, Lili HanAbstractTumor metastasis is the primary factor causing death of cancer patients and it is a study emphasis in cancer treatment to suppress tumor metastasis by inhibiting glycolysis, which is the main way of energy supply for cell mobility in tumor. In the present study, we aimed to explore the effect of quercetin, a bioactive flavonoid, on tumor metastasis and cell glycolysis and its related functionary mechanism in breast cancer progression. Firstly, trans-well invasion assay and wou...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Glucocorticoid receptor inhibit the activity of NF-κB through p38 signaling pathway in spinal cord in the spared nerve injury rats
Publication date: Available online 17 July 2018Source: Life SciencesAuthor(s): Jinping Shao, Ruiyan Xu, Ming Li, Qingzan Zhao, Xiuhua Ren, Zhihua Li, Jing Cao, Weidong ZangAbstractAimsThe glucocorticoid receptors (GRs) are an active regulator in inflammatory responses. The inflammatory reaction plays an important role in neuropathic pain, but the underlying mechanisms that GR regulates the inflammatory responses in neuropathic pain are still unknown. The activation of GRs has been shown to participate in the p38MAPK-mediated suppression of transcription activation. An unanswered question is whether GRs take part in inflamm...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Human cellular models of medium spiny neuron development and Huntington disease
Publication date: Available online 18 July 2018Source: Life SciencesAuthor(s): Monika M. GolasAbstractThe loss of gamma-aminobutyric acid (GABA)-ergic medium spiny neurons (MSNs) in the striatum is the hallmark of Huntington disease (HD), an incurable neurodegenerative disorder characterized by progressive motor, psychiatric, and cognitive symptoms. Transplantation of MSNs or their precursors represents a promising treatment strategy for HD. In initial clinical trials in which HD patients received fetal neurografts directly into the striatum without a pretransplant cell-differentiation step, some patients exhibited tempora...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Long noncoding RNA SNHG6 regulates p21 expression via activation of the JNK pathway and regulation of EZH2 in gastric cancer cells
This study indicates that SNHG6 plays an important role in GC progression via the regulation of 21. (Source: Life Sciences)
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Naringin attenuates alcoholic liver injury by reducing lipid accumulation and oxidative stress
In this study, we aim to investigate the effect and the regulatory mechanisms of naringin in the liver and whole body after alcohol exposure under zebrafish larvae system.Main methodsAt 96 h post fertilization (hpf), larvae from wild-type (WT) and transgenic zebrafish, with liver-specific eGFP expression (Tg(lfabp10α:eGFP)), were exposed to 2% ethanol for 32 h to establish an ALD model. Different endpoints, such as morphological changes in liver shape and size, histological changes, oxidative stress-related free radical levels, apoptosis and the expression of certain genes, were chosen to verify the essential imp...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Regulation of apolipoprotein A-I gene expression by the histamine H1 receptor: Requirement for NF-κB
Publication date: 1 September 2018Source: Life Sciences, Volume 208Author(s): Michael J. Haas, Marilu Jurado-Flores, Ramadan Hammoud, Gabriela Plazarte, Luisa Onstead-Haas, Norman C.W. Wong, Arshag D. MooradianAbstractAimsEarlier it had been found by us that apolipoprotein A-I (apo A-I) is suppressed by histamine in HepG2 cells. Histamine has been shown to regulate NF-κB activity, though not in hepatocytes. Therefore we examined the role of the histamine receptors and NF-κB in histamine-mediated apo A-I gene expression in HepG2 liver cells.Main methodsThe effect of histamine on histamine H1 receptor expression,...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research

Activation of AMPK prevents monocrotaline-induced pulmonary arterial hypertension by suppression of NF-κB-mediated autophagy activation
Publication date: 1 September 2018Source: Life Sciences, Volume 208Author(s): Cui Zhai, Wenhua Shi, Wei Feng, Yanting Zhu, Jian Wang, Shaojun Li, Xin Yan, Qingting Wang, Qianqian Zhang, Limin Chai, Cong Li, Pengtao Liu, Manxiang LiAbstractAimsIt has been shown that activation of autophagy is involved in the development of pulmonary arterial hypertension (PAH). Meanwhile, activation of nuclear factor-kappaB (NF-κB) has been found to induce autophagy in several types of human diseases including cancer and cardiac diseases. However, it is still unknown whether NF-κB mediates autophagy activation in PAH, and whethe...
Source: Life Sciences - July 17, 2018 Category: Biology Source Type: research

Agmatine attenuates rhabdomyolysis-induced acute kidney injury in rats in a dose dependent manner
This study was targeted to explore the prophylactic effect of agmatine (AGM), an endogenous metabolite of l-arginine against RM-induced AKI. A rat model was elucidated by 50% glycerol (10 ml/kg, im). Glycerol induced functional and structural alterations in the kidney. Pretreatment with AGM significantly ameliorated RM through decreasing total creatinine kinase (CK) and creatine kinase-MB (CK-MB) levels. AGM alleviated functional changes evidenced by decreased serum levels of creatinine (Cr), blood urea nitrogen (BUN) and decreased urinary levels of albumin and proteins. Moreover, AGM decreased renal levels of malondiald...
Source: Life Sciences - July 17, 2018 Category: Biology Source Type: research

Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effects
In conclusion; the modulatory effect of SAXA on inflammatory cytokines, its anti-apoptic properties, ameliorative impact on oxidative load and positive impact on host antioxidant defenses accounts for the observed nephro-protective impact. (Source: Life Sciences)
Source: Life Sciences - July 17, 2018 Category: Biology Source Type: research

Altered expression of Aquaporin-2 in one-kidney, one-clip hypertension
Publication date: 1 September 2018Source: Life Sciences, Volume 208Author(s): Maria Florencia Albertoni Borghese, Sandra Hope, Maria del Carmen Ortiz, Magalí Barchuk, Camila Kessler, Carlos Davio, Marcelo Vatta, Mónica MajowiczAbstractAimsThe aim of the present study was to evaluate the regulation of Aquaporin-2 (AQP2) water channel in the kidney of one-kidney, one-clip rats (Goldblatt-1 model). In addition, some mechanisms that underlie the role of AQP2 in the Goldblatt-1 model were evaluated.Main methodsSprague-Dawley rats were divided in three groups: control two-kidney, no clip (C, 2 K-NC); nephrectomiz...
Source: Life Sciences - July 17, 2018 Category: Biology Source Type: research

Asiatic acid protects differentiated PC12 cells from Aβ25–35-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling
Publication date: Available online 11 July 2018Source: Life SciencesAuthor(s): Wenjing Cheng, Weimei Chen, Peng Wang, Jianfeng ChuAbstractAmyloid β (Aβ) peptide can cause neurotoxicity in Alzheimer's disease (AD). The main purpose of the present study is to investigate the protective role of asiatic acid (AA) against Aβ25–35-induced neurotoxicity in neuronally differentiated PC12 cells. Differentiated PC12 cells were pretreated with 5, 10 or 20 μM AA before treatment with 20 μM Aβ25–35. The viability and apoptosis of differentiated PC12 cells were determined by MTT assay and Annexi...
Source: Life Sciences - July 12, 2018 Category: Biology Source Type: research

Overexpression of GRIM-19 accelerates radiation-induced osteosarcoma cells apoptosis by p53 stabilization
This study was designed to investigate the role and the underlying mechanism of GRIM-19 in osteosarcoma progression.Materials and methodsOsteosarcoma tissues and cell lines were utilized to analyze the expressions of GRIM-19 in osteosarcoma by qRT-PCR and Western blot. Methods containing flow cytometry, irradiation exposure, cells inoculation, plasmid transfection, and protein immunoprecipitation were used to investigate the underlying mechanisms of GRIM-19 in osteosarcoma progression.Key findingsGRIM-19 is downregulated in osteosarcoma tissues and cell lines. Exposure to radiation induces osteosarcoma cell apoptosis by up...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

RING finger protein 10 prevents neointimal hyperplasia by promoting apoptosis in vitro and in vivo
Publication date: Available online 30 April 2018Source: Life SciencesAuthor(s): Guiquan Yu, Jing Chen, Siyu Li, Peng Pu, Wei Huang, Yongpeng Zhao, Xin Peng, Ruiyu Wang, Han LeiAbstractAimsVascular restenosis and neointimal hyperplasia are enhanced in metabolic syndrome (MetS). Vascular smooth muscle cell (VSMC) proliferation is a key step during restenosis and is suppressed by RING finger protein 10 (RNF10). However, the effect of RNF10 on neointimal hyperplasia is unknown. In the present study, we explored whether RNF10 over-expression prevents neointimal hyperplasia in a MetS rat model and in cultured VSMCs exposed to hi...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

CXCR4 overexpression is correlated with poor prognosis in colorectal cancer
In conclusion, our findings suggest that CXCR4 might contribute to clinical tumour progression and may be a valuable prognostic biomarker in colorectal cancer treatment.Graphical abstract (Source: Life Sciences)
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells
In this study, we hypothesize that exogenous H2S may protect cardiac cells against high glucose (HG)-induced myocardial injury and inflammation with the involvement of the CIRP-MAPK signaling pathway.Main methodsH9c2 cardiac cells cultured under HG conditions were transfected with siRNA and different inhibitors for detecting the effects of sodium hydrogen sulfide (NaHS) (a H2S donor) on cell biological processes. The cardiac cell viability and LDH activity were determined by CCK-8 and LDH kit. ELISA was employed to measure the levels of inflammatory factors, while 2′,7′-dichlorofluorescein diacetate (DCFH-DA) t...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Wnt signaling in bone metastasis: mechanisms and therapeutic opportunities
Publication date: Available online 30 June 2018Source: Life SciencesAuthor(s): Xin Li, Jing Yang, Minyue Bao, Kan Zeng, Shijin Fu, Chenglin Wang, Ling YeAbstractBone metastasis frequently occurs in advanced cancer patients, who will develop osteogenic/osteolytic bone lesions in the late stage of the disease. Wnt signaling pathway, which is mainly grouped into the β-catenin dependent pathway and β-catenin independent pathway, is a well-organized cascade that has been reported to play important roles in a variety of physiological and pathological conditions, including bone metastasis. Regulation of Wnt signaling in...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Metabolome analysis of the serotonin syndrome rat model: Abnormal muscular contraction is related to metabolic alterations and hyper-thermogenesis
Publication date: Available online 2 July 2018Source: Life SciencesAuthor(s): Kei Zaitsu, Saki Noda, Akira Iguchi, Yumi Hayashi, Tomomi Ohara, Yuya Kimura, Yuta Koketsu, Tomoko Kosaki, Maiko Kusano, Takako Sato, Tetsuya Ishikawa, Hitoshi Tsuchihashi, Koichi Suzuki, Akira IshiiAbstractAimsSerotonin syndrome (SS) is an adverse outcome of selective serotonin reuptake inhibitors, though its mechanism is not understood and there is no specific clinical biomarker. In this article, metabolic profiles of the SS model rats and causes of metabolome disruption were investigated.Main methodsGas chromatography-tandem mass spectrometry ...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Corticotropin-releasing factor suppresses glioma progression by upregulation of long non-coding RNA-p21
In this study, we found CRF mRNA expression was significantly down-regulated in glioma mice. Moreover, CRF could suppress the proliferation of glioma cells and promote the expression of lincRNA-p21. Afterwards, lincRNA-p21 repressed the proliferation and invasion of glioma cells, which was reversed by miR-34c targeted with 3′-UTR. Furthermore, miR-34c decreased the expression of CRFR1 by binding with the 3′-UTR, which interact with CRF to inhibit the proliferation of glioma cells. Together, these results CRF plays as an important role in glioma progression and metastasis through activation of lincRNA-p21, provi...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Platinum-induced muscle wasting in cancer chemotherapy: Mechanisms and potential targets for therapeutic intervention
Publication date: Available online 6 July 2018Source: Life SciencesAuthor(s): Alexandra Moreira-Pais, Rita Ferreira, Rui Gil da CostaAbstractPlatinum-based drugs are among the most effective anticancer therapies, integrating the standard of care for numerous human malignancies. However, platinum-based chemotherapy induces severe side-effects in cancer patients, such as cachexia. Weight loss, as well as fatigue and systemic inflammation are characteristics of this syndrome that adversely affects the survival and the quality of life of cancer patients. The signalling pathways involved in chemotherapy-induced cachexia are sti...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Flexibility in metabolism bestows tenacious viability on cancer
Publication date: Available online 6 July 2018Source: Life SciencesAuthor(s): Yanlu Xiong, Lei Wang, Yangbo Feng, Xiaofei Li, Lintao Jia, Yong HanAbstractCancer cells display altered metabolism distinct from non-transformed cells, which is correlated closely with malignant biocharacteristics. Flexibility remains the central feature of metabolic alteration, enabling cancer cells to survive and thrive in the challenge of inner and outer environments. In this review, we summarise how cancer reprogrammes its metabolism nimbly and adaptively. To begin with, cancer cells adapt metabolism cunningly to supply sufficient materials ...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Icariin alleviates murine lupus nephritis via inhibiting NF-κB activation pathway and NLRP3 inflammasome
Publication date: Available online 6 July 2018Source: Life SciencesAuthor(s): Bofeng Su, Hong Ye, Xiaohan You, Haizhen Ni, Xuduan Chen, Linlin LiAbstractAimsLupus nephritis (LN) is a kidney inflammatory disease caused by systemic lupus erythematosus (SLE). Both NF-κB activation and NLRP3 inflammasome activation are implicated in LN pathogenesis, suggesting they are potential targets for LN treatment. Icariin, which is isolated from Chinese medicine Horny Goat Weed (Ying Yang Huo), has been shown to have anti-inflammation activity, and inhibit activations of both NF-κB and NLRP3 inflammasome. In present study, t...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Multiparametric radiomics improve prediction of lymph node metastasis of rectal cancer compared with conventional radiomics
Publication date: Available online 7 July 2018Source: Life SciencesAuthor(s): Li-Da Chen, Jin-Yu Liang, Hui Wu, Zhu Wang, Shu-Rong Li, Wei Li, Xin-Hua Zhang, Jian-Hui Chen, Jin-Ning Ye, Xin Li, Xiao-Yan Xie, Ming-De Lu, Ming Kuang, Jian-Bo Xu, Wei WangAbstractAimsTo establish multiparametric radiomics of rectal tumor for the preoperative prediction of lymph node (LN) metastasis.Materials and methodsThis prospective study consisted of 115 consecutive patients with rectal carcinoma between April 2015 and April 2017. The multiparametric radiomics scores were extracted from the endorectal ultrasound (ERUS), computed tomography...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Melatonin restrains angiogenic factors in triple-negative breast cancer by targeting miR-152-3p: In vivo and in vitro studies
Publication date: Available online 7 July 2018Source: Life SciencesAuthor(s): Jéssica H.M. Marques, André L. Mota, Jessica G. Oliveira, Jéssica Z. Lacerda, Júlia P. Stefani, Lívia C. Ferreira, Tialfi B. Castro, Andrés F. Aristizábal-Pachón, Debora A.P.C. ZuccariAbstractAimsBreast cancer represents the second most prevalent tumor-related cause of death among women. Although studies have already been published regarding the association between breast tumors and miRNAs, this field remains unclear. MicroRNAs (miRNAs) are defined as non-coding RNA molecules, and are known ...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Sini decoction alleviates E. coli induced acute lung injury in mice via equilibrating ACE-AngII-AT1R and ACE2-Ang-(1-7)-Mas axis
Publication date: Available online 7 July 2018Source: Life SciencesAuthor(s): Junjun Liu, Qiuhua Chen, Suzi Liu, Xiaoting Yang, Yan Zhang, Fengjie HuangAbstractAimsAcute respiratory distress syndrome (ARDS), one of the serious form of acute lung injury (ALI), is the primary cause of death in patients with ALI. Sini decoction (SND) is a widely used Traditional Chinese Medicine (TCM). However, the application of SND in ALI is rarely reported.Previous studies have found that renin-angiotensin-aldosterone system (RAAS) played vital and bidirectional roles in ALI. Therefore, the aim of the present study was to investigate prote...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

COX inhibitors and bone: A safer impact on osteoblasts by NO-releasing NSAIDs
Publication date: Available online 7 July 2018Source: Life SciencesAuthor(s): Maria Cristina Aisa, Alessandro Datti, Antonio Orlacchio, Gian Carlo Di RenzoAbstractNonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for the treatment of pain and inflammation. Although it is well known that NSAIDs can suppress bone growth, remodelling and repair, they are largely used post-operatively and post-traumatically to achieve analgesia and reduce inflammation in bone tissue.AimsThe impact of two NO-releasing, non-selective NSAIDs, NCX-4016 and HCT-3012 (NO-derivatives of Aspirin and Naproxen, respectively) on osteo...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Hypoxic preconditioning enhances cell hypoxia tolerance and correlated lncRNA and mRNA analysis
This study is to explore the effects of hypoxic preconditioning (HP) on osteoblasts and analyze the involved lncRNA and mRNA.Main methodsHuman osteoblast was used. Normoxic control group (N) were cultured in normoxic culture medium (L-DMEM, 10% FBS and 1% penicillin–streptomycin) 8 h. Long-term hypoxic group (H) were cultured in 4 h hypoxic culture medium (200 μmol/L CoCl2 and L-DMEM)/4 h normoxic culture medium. Hypoxic preconditioning group (HP) were received 3 times HP (cultured in 10 min hypoxic culture medium/10 min normoxic culture medium, which was HP 1times) and then cultured in 4 h hypoxic c...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Alamandine attenuates sepsis-associated cardiac dysfunction via inhibiting MAPKs signaling pathways
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Peng Li, Xi-Ru Chen, Fei Xu, Chi Liu, Chang Li, Hui Liu, Hui Wang, Wei Sun, Yan-Hui Sheng, Xiang-Qing KongAbstractSepsis-induced myocardial dysfunction represents a major cause of death. Alamandine is an important biologically active peptide. The present study evaluated whether alamandine improves cardiac dysfunction, inflammation, and apoptosis, and affects the signaling pathways involved in these events. Experiments were carried out in mice treated with lipopolysaccharide (LPS) or alamandine, and in neonatal rat cardiomyocytes. Alamandine increase...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Bisphenol a and mesenchymal stem cells: Recent insights
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Helga Caputo Nunes, Wellerson Rodrigo Scarano, Elenice Deffune, Sérgio Luis Felisbino, Immacolata Porreca, Flávia Karina DelellaAbstractMesenchymal stem cells (MSCs) are found in all adult mesenchymal tissues. They play a role in the maintenance of tissue homeostasis and repair by allowing renewal of the cellular stock. MSCs can be isolated from both human and animal sources. These cells are important in regenerative medicine and cell therapy, thus adipose tissue is a rich and promising source of these cells. Adipose-derived stem cells...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Maternal one carbon metabolism through increased oxidative stress and disturbed angiogenesis can influence placental apoptosis in preeclampsia
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Vaishali V. Kasture, Deepali P. Sundrani, Sadhana R. JoshiAbstractAdequate maternal nutrition is critical for a healthy pregnancy outcome and poor maternal nutrition is known to be associated with pregnancy complications like preeclampsia. We have earlier demonstrated that there is an imbalance in the levels of micronutrients (folate and vitamin B12) along with low levels of long chain polyunsaturated fatty acids (LCPUFA) and high homocysteine levels in women with preeclampsia. Homocysteine is known to be involved in the formation of free radicals l...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

3-Nitroacridine derivatives arrest cell cycle at G0/G1 phase and induce apoptosis in human breast cancer cells may act as DNA-target anticancer agents
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Qian Zhou, Chaoqun You, Cong Zheng, Yawen Gu, Hongchao Gu, Rui Zhang, Hongshuai Wu, Baiwang SunAbstractDNA is considered to be one of the most promising targets for anticancer agents. Acridine analogues have anticancer activity based on DNA binding and topoisomerases inhibition. However, due to the side effects, resistance and low bioavailability, a few have entered into clinical usage and the mechanisms of action are not fully understood. Novel acridine derivatives are needed for effective cancer therapy. A series of novel 3-nitroacridine-based der...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Preparation of an efficient and safe polymeric-magnetic nanoparticle delivery system for sorafenib in hepatocellular carcinoma
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Greeshma Tom, Sheena Philip, Rimal Isaac, P.K. Praseetha, S.G. Jiji, V.V. AshaAbstractAimsSuperparamagnetic iron oxide nanoparticles (SPIONs), as drug delivery vehicles, offer to eliminate the concerns associated with hydrophobic anti-cancer agents. The current study was intended to fabricate a SPION based delivery system for sorafenib that can simultaneously enable targeted delivery of sorafenib and expand its therapeutic index against hepatocellular carcinoma (HCC).Main methodsCo-precipitation and physical entrapment methods were employed for the ...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

18F-Fluoride PET/CT and 99mTc-MDP SPECT/CT can detect bone cancer at early stage in rodents
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Christiano R.R. Alves, Daniele de P. Faria, Camila de G. Carneiro, Alexandre T. Garcez, Vanessa P. Gutierrez, Willian das Neves, Ney R. de Almeida, Yara Cury, Roger Chammas, Patricia C. BrumAbstractNoninvasive imaging using positron emission tomography/computed tomography (PET/CT) and single photon emission computed tomography/computed tomography (SPECT/CT) are considered revolutionized approaches to detect bone cancer. Both PET/CT and SPECT/CT technologies have advanced to permit miniaturization, which has provided the advantage of including animal...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Oxyfadichalcone C inhibits melanoma A375 cell proliferation and metastasis via suppressing PI3K/Akt and MAPK/ERK pathways
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Xiaolin Peng, Zhengming Wang, Yang Liu, Xin Peng, Yao Liu, Shan Zhu, Zhe Zhang, Yuling Qiu, Meihua Jin, Ran Wang, Qingying Zhang, Dexin KongAbstractAimsMelanoma remains to be one of the most incurable cancers. Discovery of novel antitumor agent for melanoma therapy is expected. We recently isolated Oxyfadichalcone C from Oxytropis falcate and investigated the anti-proliferative and anti-metastatic activity on human melanoma A375 cells in vitro.Main methodsCell viability was determined using MTT assay and soft agar cloning formation assay. The effect...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Deoxyelephantopin ameliorates lipopolysaccharides (LPS)-induced memory impairments in rats: Evidence for its anti-neuroinflammatory properties
In this study, DET (0.625. 1.25 and 2.5 mg/kg, i.p.) was administered in rats for 21 days and those animals were challenged with single injection of LPS (250 μg/kg, i.p.) for 7 days. Cognitive and behavioral assessment was carried out for 7 days followed by molecular assessment on brain hippocampus. Statistical significance was analyzed with one-way analysis of variance followed by Dunnett's test to compare the treatment groups with the control group.Key findingsDET ameliorated LPS-induced neuroinflammation by suppressing major pro-inflammatory mediators such as iNOS and COX-2. Furthermore, DET enhanced the an...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Neuralized1a regulates asymmetric division in mouse Lewis lung carcinoma cells
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Liangsheng Kong, Jingyuan Li, Yongli Liu, Zhiwei Sun, Shixia Zhou, Junling Tang, Ting Ye, Jianyu Wang, H. Rosie XingAbstractAsymmetric division (ASD), the unique characteristic of normal stem cells, is regarded as a stemness marker when applied to the study of cancer stem cells (CSCs). However, the role of ASD in the self-renewal of CSCs and its regulation remain largely unknown. Here, we first established a mouse Lewis lung carcinoma CSC cell line that could undergo asymmetric division (LLC-ASD cells) derived from the parental mouse Lewis lung carc...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Astragaloside suppresses apoptosis of the podocytes in rats with diabetic nephropathy via miR-378/TRAF5 signaling pathway
This study aims to explore the effect of AS-IV on diabetic nephropathy progression.Materials and methodsThe diabetic nephropathy model was established in rats with streptozotocin (STZ) injection. The albuminuria was examined by using the enzyme linked immunosorbent assay (ELISA). The expression of miR-378, tumor-necrosis factor (TNF) receptor (TNFR)-associated factor 5 (TRAF5) mRNA and protein was analyzed by qRT-PCR and western blot, respectively. Cell transfection was conducted for modulating endogenous expression of miR-378. Dual luciferase reporter assay was used to evaluate the interaction between miR-378 and TRAF5. T...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Puerarin attenuates palmitate-induced mitochondrial dysfunction, impaired mitophagy and inflammation in L6 myotubes
This study was conducted to analyze the attributive properties of puerarin against mitochondrial dysfunction and inflammation in skeletal muscle cells with insulin resistance.Main methodsThe effects of puerarin on mitochondrial biogenesis, oxidative phosphorylation, dynamics of fusion, fission and mitophagy, oxidative stress, as well as inflammatory response and insulin sensitivity in L6 myotubes treated with palmitate were examined.Key findingsPuerarin pretreatment improve insulin sensitivity and prevented muscle cells from palmitate-induced mitochondrial dysfunction manifested by the increases of complex I activity, mito...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Resistance training attenuates inflammation and the progression of renal fibrosis in chronic renal disease
In conclusion, RT was effective in reducing fibrosis and inflammation, in addition to increasing muscle strength and creatine kinase levels, in rats with CKD, independent of muscle mass. (Source: Life Sciences)
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Expression analysis of a cholecystokinin system in human and rat white adipose tissue
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Adrián Plaza, Beatriz Merino, Andrés Sánchez-Pernaute, Antonio J. Torres-García, Miguel A. Rubio-Herrera, Mariano Ruiz-GayoAbstractAimCholecystokinin (CCK) participates in the storage of dietary triglycerides in white adipose tissue (WAT). Our goal was to characterize, both in subcutaneous (Sc-WAT) and visceral WAT (Vis-WAT), the functional expression of the two known CCK receptors, CCK-1 (CCK-1R) and CCK-2 (CCK-2R), as well as of CCK.Main methodsGene and protein expression was assessed in different cell types of rat and ...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats
ConclusionsOur results demonstrated that PAH exhibited antidepressant-like effect in CUMS-induced rats model of depression, which might be mediated by TXNIP/TRX/NLRP3 pathway. (Source: Life Sciences)
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research