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Total 656 results found since Jan 2013.
Amphiphilic small peptides for delivery of plasmid DNAs and siRNAs
This article is protected by copyright. All rights reserved.
As novel nucleic acid carriers, linear and branched amphiphilic peptides were prepared. Each peptide was compared in terms of SNALP size, stability, and delivery activities for plasmid DNA (pDNA) and siRNA. Among the five peptides, a linear peptide H‐RCL was an effective delivery carrier for both siRNA and pDNA. Introduction of a benzoyl group to H‐RCL significantly enhanced siRNA delivery, but greatly reduced pDNA delivery. These demonstrated that a small structural modification of the peptides can modulate their delivery properties.
Source: Chemical Biology and Drug Design - October 20, 2017 Category: Biology Authors: Eun ‐Kyoung Bang, Hanna Cho, Sean S‐H Jeon, Na Ly Tran, Dong‐Kwon Lim, Wooyoung Hur, Taebo Sim Tags: Research Article Source Type: research
Solid Phase Synthesis and Self ‐Assembly of Higher‐Order siRNAs and their Bioconjugates
This article is protected by copyright. All rights reserved.
Source: Chemical Biology and Drug Design - November 27, 2018 Category: Biology Authors: Christopher N. Cultrara,
Sunil Shah,
Stephen D. Kozuch,
Mayurbhai R. Patel,
David Sabatino Tags: Special Issue Article Source Type: research
Small interfering RNA targeting alpha7 nicotinic acetylcholine receptor sensitizes hepatocellular carcinoma cells to sorafenib
Publication date: Available online 18 January 2020Source: Life SciencesAuthor(s): Khalil Hajiasgharzadeh, Mohammad Hossein Somi, Behzad Mansoori, Vahid Khaze Shahgoli, Afshin Derakhshani, Ahad Mokhtarzadeh, Dariush Shanehbandi, Behzad BaradaranAbstractAimsIt has been demonstrated that reduced expression of alpha7 nicotinic acetylcholine receptor (α7nAChR) led to reduced chemotherapeutic drugs resistance in various cancer cells. However, whether small interfering RNA (siRNA) mediated knockdown of α7nAChR can reduce sorafenib (SOR) resistance in HCC cells remains to be determined.Materials and methodsThe effects of α7nACh...
Source: Life Sciences - January 18, 2020 Category: Biology Source Type: research
Endo-PDI is required for TNFα-induced angiogenesis.
Conclusion: Our study establishes Endo-PDI as a novel, important mediator of AP-1 driven gene expression and endothelial angiogenic function.
PMID: 24103565 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - October 5, 2013 Category: Biology Authors: de Lucca Camargo L, Babelova A, Mieth A, Weigert A, Mooz J, Rajalingam K, Heide H, Wittig I, Rossetti Lopes L, Brandes RP Tags: Free Radic Biol Med Source Type: research
P2Y2R activation by nucleotides released from oxLDL-treated endothelial cells (ECs) mediates the interaction between ECs and immune cells through RAGE expression and reactive oxygen species production.
Abstract
Lipoprotein oxidation, inflammation, and immune responses involving the vascular endothelium and immune cells contribute to the pathogenesis of atherosclerosis. In an atherosclerotic animal model, P2Y2 receptor (P2Y2R) upregulation and stimulation were previously shown to induce intimal hyperplasia and increased intimal monocyte infiltration. Thus, we investigated the role of P2Y2R in oxidized low-density lipoprotein (oxLDL)-mediated oxidative stress and the subsequent interaction between endothelial cells (ECs) and immune cells. The treatment of human ECs with oxLDL caused the rapid release of ATP (maxim...
Source: Free Radical Biology and Medicine - January 29, 2014 Category: Biology Authors: Eun SY, Park SW, Lee JH, Chang KC, Kim HJ Tags: Free Radic Biol Med Source Type: research
Dynamic parent-of-origin effects on small interfering RNA expression in the developing maize endosperm
Conclusions:
A subset of siRNAs subjected to imprinted expression pattern in maize developing endosperm, and they are likely correlated with certain imprinted gene expression. Additionally, siRNAs might influence nutrient uptake and allocation processes during maize endosperm development.
Source: BMC Plant Biology - Latest articles - July 24, 2014 Category: Biology Authors: Mingming XinRuolin YangYingyin YaoChuang MaHuiru PengQixin SunXiangfeng WangZhongfu Ni Source Type: research
Intratracheal administration of mitochondrial DNA directly provokes lung inflammation through the TLR9-p38 MAPK pathway.
CONCLUSION: The intratracheal administration of mtDNA induced a local inflammatory response in the mouse lungs that depended on the interactions of mtDNA with TLR9 and may be correlated with infiltrating macrophages that could be activated by mtDNA exposure via the TLR9-p38 MAPK signal transduction pathway.
PMID: 25772007 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - March 12, 2015 Category: Biology Authors: Gu X, Wu G, Yao Y, Zeng J, Shi D, Lv T, Luo L, Song Y Tags: Free Radic Biol Med Source Type: research
Influence of various stressors to the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis (Hendel)
This article is protected by copyright. All rights reserved
Source: Insect Science - January 6, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research
Influence of various stressors on the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis
Abstract
RNA interference (RNAi)‐based technology has emerged as a potential tool for controlling insect pests, however, previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable. In nature, insects often meet various challenges, such as pathogen infections, extreme temperatures, lack of nutrition and heavy metals. To better understand the association of the stressors with efficiency of RNAi, in the current study we tested the expression of three core genes, dicer2 (Bddcr2), r2d2 (Bdr2d2) and argonaute2 (Bdago2), of the small interfering RNA (siRNA) pathway of B. dorsalis upon various stresso...
Source: Insect Science - May 24, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research
RNA-induced silencing attenuates G protein-mediated calcium signals Research Communication
Phospholipase Cβ (PLCβ) is activated by G protein subunits in response to environmental stimuli to increase intracellular calcium. In cells, a significant portion of PLCβ is cytosolic, where it binds a protein complex required for efficient RNA-induced silencing called C3PO (component 3 promoter of RISC). Binding between C3PO and PLCβ raises the possibility that RNA silencing activity can affect the ability of PLCβ to mediate calcium signals. By use of human and rat neuronal cell lines (SK-N-SH and PC12), we show that overexpression of one of the main components of C3PO diminishes Ca2+ release in r...
Source: FASEB Journal - April 28, 2016 Category: Biology Authors: Philip, F., Sahu, S., Golebiewska, U., Scarlata, S. Tags: Research Communication Source Type: research
The Mitochondria-targeted Antioxidant MitoQ Ameliorated Tubular Injury Mediated by Mitophagy in Diabetic Kidney Disease via Nrf2/PINK1
Publication date: Available online 21 December 2016 Source:Redox Biology Author(s): Li Xiao, Xiaoxuan Xu, Fan Zhang, Ming Wang, Yan Xu, Dan Tang, Jiahui Wang, Yan Qin, Yu Liu, Chengyuan Tang, Liyu He, Anna Greka, Zhiguang Zhou, Fuyou Liu, Dong Zeng, Lin Sun Mitochondria play a crucial role in tubular injury in diabetic kidney disease (DKD). MitoQ is a mitochondria-targeted antioxidant that exerts protective effects in diabetic mice, but the mechanism underlying these effects is not clear. We demonstrated that mitochondrial abnormalities, such as defective mitophagy, mitochondrial reactive oxygen species (ROS) overexpressi...
Source: Redox Biology - December 20, 2016 Category: Biology Source Type: research
Gene Silencing of Selenoprotein K Induces Inflammatory Response and Activates Heat Shock Proteins Expression in Chicken Myoblasts.
Abstract
In the present study, specific small interfering RNA (siRNA) for selenoprotein K (Selk) gene was designed and transfected into chicken myoblasts. Then, the expressions of inflammatory factors (including induced nitric oxide synthase [iNOS], nuclear factor-kappa B [NF-κB], heme-oxygenase-1 [HO-1], cyclooxygenase-2 [COX-2], and prostaglandin E synthase [PTGEs]), inflammation-related cytokines (including interleukin [IL]-1β, IL-6, IL-7, IL-8, IL-17, and interferon [IFN]-γ), and heat shock proteins (HSPs) (including HSP27, HSP40, HSP60, HSP70, and HSP90) were examined at 24 and 72 h after transfection. Th...
Source: Biological Trace Element Research - March 8, 2017 Category: Biology Authors: Fan R, Yao H, Cao C, Zhao X, Khalid A, Zhao J, Zhang Z, Xu S Tags: Biol Trace Elem Res Source Type: research
Endothelial deletion of mTORC1 protects against hindlimb ischemia in diabetic mice via activation of autophagy, attenuation of oxidative stress and alleviation of inflammation.
In conclusion, our present study demonstrates that endothelial mTORC1 deletion protects against hindlimb ischemic injury in diabetic mice possibly via activation of autophagy, attenuation of oxidative stress and alleviation of inflammation. Therapeutics targeting mTORC1 may therefore represents a promising strategy to rescue limb ischemia in diabetes mellitus.
PMID: 28473248 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - May 1, 2017 Category: Biology Authors: Fan W, Han D, Sun Z, Ma S, Gao L, Chen J, Li X, Li X, Fan M, Li C, Hu D, Wang Y, Cao F Tags: Free Radic Biol Med Source Type: research
Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells
Publication date: Available online 7 February 2019Source: Life SciencesAuthor(s): Huojun Zhang, Qian Liu, Luxia Kong, Shuyun XuAbstractAimsTo investigate whether mucin 1 (MUC1) downregulation reduced the sensitivity of tumor necrosis factor-alpha (TNF-α)-induced bronchial epithelial cells to glucocorticoid-mediated necroptosis and explore the underlying mechanisms.Main methodsThe human lung bronchial epithelial cell line (16HBE) was transfected with small interfering RNA (siRNA) against MUC1 and then stimulated by TNF-α, where some cells were pretreated with dexamethasone. Flow cytometry was performed to analyze necropto...
Source: Life Sciences - February 7, 2019 Category: Biology Source Type: research
Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer
Publication date: Available online 19 April 2019Source: Life SciencesAuthor(s): Jiaxin Zuo, Yameng Jiang, Enxia Zhang, Yuling Chen, Ze Liang, Jie Zhu, Yinan Zhao, Hong Xu, Guoliang Liu, Jiasi Liu, Wei Wang, Shubiao Zhang, Yuhong ZhenAbstractAimsTo investigate the antitumor effect of 7-O-geranylquercetin (GQ) combining with survivin siRNA (siSuvi) or IL-10 siRNA (siIL-10) to breast cancer.Main methodsXenograft tumor model was established by subcutaneously inoculating human breast cancer MCF-7 cells in BALB/c nude mice. Transfection efficiency of siRNA mediated by cationic liposome CDO14 in MCF-7 cells and tumor bearing mice...
Source: Life Sciences - April 19, 2019 Category: Biology Source Type: research
