Aspartate ‐β‐alanine‐NBAD pathway regulates pupal melanin pigmentation plasticity of ladybird Harmonia axyridis
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - October 16, 2020 Category: Biology Authors: Yi Zhang, Xing ‐Xing Wang, Zhu‐Jun Feng, Hong‐Gang Tian, Yi Feng, Tong‐Xian Liu Tags: ORIGINAL ARTICLE Source Type: research

Roles of LmCDA1 and LmCDA2 in cuticle formation in the foregut and hindgut of Locusta migratoria
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - October 10, 2020 Category: Biology Authors: Min Zhang, Peng ‐Juan Ma, Ting‐Ting Zhang, Zhi‐Mei Gao, Pan Zhao, Xiao‐Jian Liu, Xue‐Yao Zhang, Wei‐Min Liu, Rong‐Rrong Yu, Bernard Moussian, Jian‐Zhen Zhang Tags: ORIGINAL ARTICLE Source Type: research

A CCCH zinc finger gene regulates doublesex alternative splicing and male development in Bombyx mori
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - October 7, 2020 Category: Biology Authors: Fangying Yang, Zhongjie Zhang, Bo Hu, Ye Yu, Anjiang Tan Tags: ORIGINAL ARTICLE Source Type: research

Novel strategies for the biocontrol of noctuid pests (Lepidoptera) based on improving ascovirus infectivity using Bacillus thuringiensis
This article is protected by copyright. All rights reservedAbstractIdentifying novel biocontrol agents and developing new strategies are urgent goals in insect pest biocontrol. Ascoviruses are potential competent insect viruses that may be developed into bioinsecticides, but this aim is impeded by their poor oral infectivity. To improve theper os infectivity of ascovirus,Bacillus thuringiensis kurstaki (Btk) was employed as a helper to damage the midgut of lepidopteran larvae (Helicoverpa armigera,Mythimna separata,Spodoptera frugiperda, andS. litura) in formulations withHeliothis virescens ascovirus isolates (HvAV ‐3h a...
Source: Insect Science - October 4, 2020 Category: Biology Authors: Huan Yu, Chang ‐Jin Yang, Ni Li, Ying Zhao, Zhuang‐Mei Chen, Si‐Jia Yi, Zi‐Qi Li, Michael J. Adang, Guo‐Hua Huang Tags: ORIGINAL ARTICLE Source Type: research

Genome ‐wide identification and characterization of long non‐coding RNAs in Tribolium castaneum
This study provides comprehensive information for lncRNAs ofT. castaneum, and will promote functional analysis and target identification of lncRNAs in the insect. (Source: Insect Science)
Source: Insect Science - September 24, 2020 Category: Biology Authors: Liu Yang, You ‐Wei Wang, Yao‐Yao Lu, Bin Li, Ke‐Ping Chen, Cheng‐Jun Li Tags: ORIGINAL ARTICLE Source Type: research

Domestication ‐related changes in sexual performance of Queensland fruit fly
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 22, 2020 Category: Biology Authors: Jeanneth P érez, Vivian Mendez, Boaz Yuval, Phillip W. Taylor Tags: ORIGINAL ARTICLE Source Type: research

Regulatory mechanisms of microbial homeostasis in insect gut
Intestinal symbionts are important for the growth, development and reproduction of insects; resisting pathogens and maintaining the homeostasis of the gut microbial are important for insects.Intestinal physical barrier, Imd, Duox ‐ROS, JAK/STAT signaling pathways and intestinal symbiotic flora are beneficial to insect resistance to pathogens and maintenance the homeostasis of the gut microbiome. AbstractInsects live in incredibly complex environments. The intestinal epithelium of insects is in constant contact with microorganisms, some of which are beneficial and some harmful to the host. Insect gut health and function a...
Source: Insect Science - September 22, 2020 Category: Biology Authors: Shuai Bai, Zhichao Yao, Muhammad Fahim Raza, Zhaohui Cai, Hongyu Zhang Tags: REVIEW Source Type: research

Domestication ‐related changes in sexual performance of Queensland fruit fly
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 22, 2020 Category: Biology Authors: Jeanneth P érez, Vivian Mendez, Boaz Yuval, Phillip W. Taylor Tags: ORIGINAL ARTICLE Source Type: research

Symbiotic bacteria attenuate Drosophila oviposition repellence to alkaline through acidification
In conclusion,Enterococcus protectsDrosophila from alkali stress by acidifying the ovipositional substrate, and ultimately improves the fitness of theDrosophila population. Our results demonstrate that symbionts are profound factors in theDrosophila ovipositional decision, and extend our understanding of the intimate interactions betweenDrosophila and their symbionts. (Source: Insect Science)
Source: Insect Science - September 17, 2020 Category: Biology Authors: Wei Liu, Jie Wang, Hong ‐Yu Zhang, Ying‐Chun Yang, Ru‐Xue Kang, Peng Bai, Hui Fu, Li‐Rong Chen, Yan‐Ping Gao, Eng King Tan Tags: ORIGINAL ARTICLE Source Type: research

Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)
This study showed that some specificCPAP3 genes had crucial roles inB. dorsalis development, and these CP genes could be used as potential targets to control this pest via RNAi. (Source: Insect Science)
Source: Insect Science - September 17, 2020 Category: Biology Authors: Qiu ‐Li Hou, Er‐Hu Chen, Wei Dou, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

CRISPR/Cas9 ‐based functional analysis of yellow gene in the diamondback moth, Plutella xylostella
We identified a gene of pigmentation (yellow) in P. xylostella, Pxyellow, and performed a clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR ‐associated protein 9 (Cas9)‐mediated knockout experiment to verify thatPxyellow plays an essential role in the body pigmentation without being directly involved in regulation of growth, development, and reproduction. Such findings prove the effectiveness ofPxyellow as a screening marker for genetically based pest control techniques. AbstractThe diamondback moth,Plutella xylostella (L.), is an economically important pest of cruciferous crops worldwide. This pest ...
Source: Insect Science - September 17, 2020 Category: Biology Authors: Yajun Wang, Yuping Huang, Xuejiao Xu, Zhaoxia Liu, Jianyu Li, Xue Zhan, Guang Yang, Minsheng You, Shijun You Tags: LETTER TO THE EDITOR Source Type: research

CRISPR/Cas9 ‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori
Circadian rhythms, which are ubiquitous and adaptive, occur across all species, from microbes to humans, in which they organize and modify behavior and physiology.timeless (tim) is a canonical clock gene. The core composition of theDrosophila melanogaster endogenous circadian clock has been extensively investigated; however, in lepidopteran insects, includingBombyx mori, the mechanism is complicated and little is known regarding the participation oftim in the negative feedback loop responsible for behavioral activities. To arrive at a comprehensive understanding of the role oftim in theB. mori endogenous circadian clock, w...
Source: Insect Science - September 17, 2020 Category: Biology Authors: Moses Addo Nartey, Xia Sun, Sheng Qin, Cheng ‐Xiang Hou, Mu‐Wang Li Tags: ORIGINAL ARTICLE Source Type: research

An InR/mir ‐9a/NlUbx regulatory cascade regulates wing diphenism in brown planthoppers
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 15, 2020 Category: Biology Authors: Xiang Li, Mu ‐Hua Zhao, Miao‐Miao Tian, Jing Zhao, Wan‐Lun Cai, Hong‐Xia Hua Tags: ORIGINAL ARTICLE Source Type: research

Molecular basis of the silkworm mutant rel causing red egg color and embryonic death
This study revealed the molecular basis of silkwormrel mutant formation and indicated that theSema1a gene is essential for insect embryo development.This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 10, 2020 Category: Biology Authors: Jiang ‐Wen Luo, Er‐Xia An, Ya‐Ru Lu, Ling Yang, Ting‐Ting Gai, Song‐Zhen He, Song‐Yuan Wu, Hai Hu, Chun‐Lin Li, Cheng Lu, Xiao‐Ling Tong, Fang‐Yin Dai Tags: ORIGINAL ARTICLE Source Type: research

Trajectory modeling revealed a southwest ‐northeast migration corridor for fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) emerging from the North China Plain
Graphical Abstract: Trajectory modeling revealed a southwest ‐northeast migration corridor for fall armyworm (Spodoptera frugiperda J. E. Smith) (Lepidoptera: Noctuidae) emerging from the North China Plain. The spatiotemporal extent of the nocturnal migratory movements of fall armyworm from the North China Plain was simulated using the trajectory analysis approach based on historical meteorological data from June to October of 2015 ‐2019. The topography‐induced windborne displacement of newly emerged moths from the North China Plain was concentrated in Liaoning, Jilin and Heilongjiang provinces before late summer, an...
Source: Insect Science - September 9, 2020 Category: Biology Authors: Qiu ‐Lin Wu, Yu‐Ying Jiang, Jie Liu, Gao Hu, Kong‐Ming Wu Tags: ORIGINAL ARTICLE Source Type: research

An inducible constitutive expression system in Bombyx mori mediated by phiC31 integrase
Graphical Abstract The present study reports a phiC31 integrase ‐mediated promoter flip system (FLIP) via the attB/attP exchange for the inducible expression of target genes in silkworms. Left inset illustrates the schematic of the FLIP system mediated by the phiC31 integrase‐induced attB/attP exchange. Right inset shows this system activates the efficient a nd inducible expression of DsRed in a silkworm embryo‐derived cell line BmE and silkworm individuals. AbstractInducible gene ‐expression systems play important roles in gene functional assays in the post‐genome era.Streptomyces phage ‐derived phiC31 integra...
Source: Insect Science - September 9, 2020 Category: Biology Authors: Feng Wang, Yan ‐Ting Ji, Chi Tian, Yuan‐Cheng Wang, Shen Xu, Ri‐Yuan Wang, Qian‐Qian Yang, Ping Zhao, Qing‐You Xia Tags: ORIGINAL ARTICLE Source Type: research

Diversity and functional analysis of Chinese bumblebee gut microbiota reveal the metabolic niche and antibiotic resistance variation of Gilliamella
AbstractBumblebees play an important role in maintaining the balance of natural and agricultural ecosystems, and the characteristic gut microbiota of bumblebees exhibit significant mutualistic functions. China has the highest diversity of bumblebees; however, gut microbiota of Chinese bumblebees have mostly been investigated through culture ‐independent studies. Here, we analyzed the gut communities of bumblebees from Sichuan, Yunnan, and Shaanxi provinces in China through 16S ribosomal RNA amplicon sequencing and bacterial isolation. It revealed that the bumblebees examined in this study harbored two gut enterotypes as ...
Source: Insect Science - September 8, 2020 Category: Biology Authors: Zi ‐Jing Zhang, Ming‐Fei Huang, Li‐Fei Qiu, Rui‐Hao Song, Zi‐Xuan Zhang, Yi‐Wen Ding, Xin Zhou, Xue Zhang, Hao Zheng Tags: ORIGINAL ARTICLE Source Type: research

CRISPR/Cas9 ‐based functional analysis of yellow gene in the diamondback moth, Plutella xylostella
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 6, 2020 Category: Biology Authors: Yajun Wang, Yuping Huang, Xuejiao Xu, Zhaoxia Liu, Jianyu Li, Xue Zhan, Guang Yang, Minsheng You, Shijun You Tags: LETTER TO THE EDITOR Source Type: research

Regulatory mechanisms of microbial homeostasis in insect gut
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - September 3, 2020 Category: Biology Authors: Shuai Bai, Zhi ‐Chao Yao, Muhammad Fahim Raza, Zhao‐Hui Cai, Hong‐Yu Zhang Tags: REVIEW Source Type: research

Transcriptional identification of differentially expressed genes associated with division of labor in Apis cerana cerana
AbstractWorkers ofApis cerana cerana undergo an in ‐hive nursing to outdoor foraging transition, but the genes underlying this age‐related transition remain largely unknown. Here, we sequenced the head transcriptomes of its 7‐day‐old normal nurses, 18‐ and 22‐day‐old normal foragers, 7‐day‐old precocious foragers and 22‐day‐old over‐aged nurses to unravel the genes associated with this transition. Mapping of the sequence reads toApis mellifera genome showed that the three types of foragers had a greater percentage of reads from annotated exons and intergenic regions, whereas the two types of nurses ...
Source: Insect Science - September 2, 2020 Category: Biology Authors: Yi ‐Jie Chen, Ying‐Jiao Li, Shuang Wu, Wen‐Chao Yang, Jing Miao, Shao‐Hua Gu, Jiang‐Hong Li, Xiao‐Qing Miao, Xianchun Li Tags: ORIGINAL ARTICLE Source Type: research

Decline of Eulia ministrana (Lepidoptera: Tortricidae) in polluted habitats is not accompanied by phenotypic stress responses
The population density of a leafrolling mothEulia ministrana decreased 10 fold with a 15 fold increase in environmental pollution, but this decline was not accompanied by the expected changes in the studied morphological stress indices: size, forewing melanization and fluctuating asymmetry in wing venation. Thus, insect populations can decline in polluted habitats without any visible signs of stress. AbstractEnvironmental pollution is currently identified as one of the major drivers of rapid decline of insect populations, and this finding has revitalized interest in insect responses to pollution. We tested the hypothesis t...
Source: Insect Science - August 31, 2020 Category: Biology Authors: Vitali Zverev, Mikhail V. Kozlov Tags: ORIGINAL ARTICLE Source Type: research

Only mothers feed mature offspring in European Ceratina bees
‐ Feeding of mature offspring is an uncommon type of care about offspring but usual in small carpenter bees (Ceratina).‐ We detected feeding of offspring by mother in three EuropeanCeratina bees.‐ However, sisters do not care about siblings compared to some other species of the same genus. AbstractParental care directed to adult offspring is uncommon in animals. Such parental care has been documented in Xylocopinae bees (Hymenoptera: Apidae). Moreover, someCeratina bees (Xylocopinae) are known to feed mature siblings, and feeding of mature siblings is achieved by dwarf eldest daughters when mothers died. These daught...
Source: Insect Science - August 27, 2020 Category: Biology Authors: Michael Mik át, Jitka Waldhauserová, Tereza Fraňková, Kateřina Čermáková, Vojtěch Brož, Šimon Zeman, Marcela Dokulilová, Jakub Straka Tags: ORIGINAL ARTICLE Source Type: research

The effect of the endosymbiont Wolbachia on the behavior of insect hosts
AbstractAs one of the most successful intracellular symbiotic bacteria,Wolbachia can infect many arthropods and nematodes.Wolbachia infection usually affects the reproduction of their hosts to promote their own proliferation and transmission. Currently, most of the studies focus on the mechanisms ofWolbachia interactions with host reproduction. However, in addition to distribution in the reproductive tissues,Wolbachia also infect various somatic tissues of their hosts, including the brain. This raises the potential thatWolbachia may influence some somatic processes, such as behaviors in their hosts. So far, information abo...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Jie Bi, Yu ‐Feng Wang Tags: REVIEW Source Type: research

Cover Caption
Spider mites are notorious agricultural pests worldwide. A male adult ofTetranychus truncatus is waiting for mating with a female. Spider mites harbor diverse bacterial communities, whose structure could be affected by many factors. We found that host species is the primary determinant of the microbiome composition of spider mites. Also, the microbiome of spider mite feces potentially reflects the gut bacterial community in spider mites (see pages 859 ‐868). Photo provided by Xiao‐Yue Hong of Nanjing Agricultural University, China. (Source: Insect Science)
Source: Insect Science - August 26, 2020 Category: Biology Tags: CONTENTS Source Type: research

Issue Information
Insect Science, Volume 27, Issue 5, Page 845-845, October 2020. (Source: Insect Science)
Source: Insect Science - August 26, 2020 Category: Biology Tags: ISSUE INFORMATION Source Type: research

Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high ‐throughput amplicon sequencing
AbstractThe microbiome associated with brown planthopper (BPH) plays an important role in mediating host health and fitness. Characterization of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host insect. Here, we investigated the bacterial and fungal communities of BPH at different developmental stages using high ‐throughput amplicon sequencing. Our results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH development. The bacterial communities were generally richer than fungi in each develop...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Zheng ‐Liang Wang, Tian‐Zhao Wang, Hang‐Feng Zhu, Hai‐Bo Pan, Xiao‐Ping Yu Tags: ORIGINAL ARTICLE Source Type: research

Wolbachia ‐induced expression of kenny gene in testes affects male fertility in Drosophila melanogaster
AbstractWolbachia are Gram ‐negative endosymbionts that are known to cause embryonic lethality when infected male insects mate with uninfected females or with females carrying a different strain ofWolbachia, a situation characterized as cytoplasmic incompatibility (CI). However, the mechanism of CI is not yet fully understood, although recent studies onDrosophila melanogaster have achieved great progress. Here, we found thatWolbachia infection caused changes in the expressions of several immunity ‐related genes, including significant upregulation ofkenny (key), in the testes ofD. melanogaster. Overexpression ofkey in f...
Source: Insect Science - August 26, 2020 Category: Biology Authors: John C. Biwot, Hua ‐Bao Zhang, Chen Liu, Jun‐Xue Qiao, Xiao‐Qiang Yu, Yu‐Feng Wang Tags: ORIGINAL ARTICLE Source Type: research

Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations
This study investigated the bacterial and fungal community compositions of Chinese wi ld rice planthoppersLaodelphax striatellus andSogatella furcifera using parallel 16S rRNA gene amplicon and internal transcribed space region sequencing. The bacteria varied significantly between the species and were partitioned significantly by sex, tissues and host environments in each species. The majority of bacteria were affiliated with the generaWolbachia,Cardinium,Rickettsia andPantoea. The abundance ofWolbachia was negatively correlated with that ofCardinium in both planthopper species. Compared with bacteria, the abundance and di...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Xiao ‐Li Bing, Dian‐Shu Zhao, Chang‐Wu Peng, Hai‐Jian Huang, Xiao‐Yue Hong Tags: ORIGINAL ARTICLE Source Type: research

Assessing immunocompetence in red palm weevil adult and immature stages in response to bacterial challenge and entomopathogenic nematode infection
AbstractParasites and pathogens can follow different patterns of infection depending on the host developmental stage or sex. In fact, immune function is energetically costly for hosts and trade ‐offs exist between immune defenses and life history traits as growth, development and reproduction and organisms should thus optimize immune defense through their life cycle according to their developmental stage. Identifying the most susceptible target and the most virulent pathogen is particula rly important in the case of insect pests, in order to develop effective control strategies targeting the most vulnerable individuals w...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Federico Cappa, Giulia Torrini, Giuseppe Mazza, Alberto Francesco Inghilesi, Claudia Benvenuti, Leonardo Viliani, Pio Federico Roversi, Rita Cervo Tags: ORIGINAL ARTICLE Source Type: research

Cellular and humoral immune interactions between Drosophila and its parasitoids
Parasitoid infestation inDrosophila is counteracted by its multiple natural immune defense systems, including cellular and humoral immunity.Cellular immune responses involve the proliferation, differentiation, migration and spreading of host hemocytes and parasitoid encapsulation by them.Humoral immune responses rely more heavily on melanization and on the Toll, Imd and Jak/Stat immune pathways associated with antimicrobial peptides along with stress factors.To achieve successful development, various virulence factors are introduced while laying eggs, among which venom and virus ‐like particles (VLPs) are the key players...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Lei Yang, Li ‐Ming Qiu, Qi Fang, David W. Stanley, Gong‐Yin Ye Tags: REVIEW Source Type: research

Myosuppressin influences fecundity in the Colorado potato beetle, Leptinotarsa decemlineata
In this study, we investigated the involvement of MS in fecundity in a notorious defoliator of potato and other solanaceous plants, the Colorado potato beetle (CPB),  Leptinotarsa decemlineata. We identified an MS ‐precursor‐encoding transcript in theL. decemlineata transcriptomic database and then evaluated its transcript levels in various CPB tissues. MS transcript levels were found to be highest in the central nervous system, gut and muscle of CPB males and females. To investigate the role of MS in fecundity, MS was silenced in adult CPBs through RNA interference (RNAi). This resulted in a significant reduction...
Source: Insect Science - August 26, 2020 Category: Biology Authors: Shun ‐Hua Gui, Clauvis Nji Tizi Taning, Guy Smagghe Tags: ORIGINAL ARTICLE Source Type: research

Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)
This study showed that some specificCPAP3 genes had crucial roles inB. dorsalis development, and these CP genes could be used as potential targets to control this pest via RNAi.This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - August 26, 2020 Category: Biology Authors: Qiu ‐Li Hou, Er‐Hu Chen, Wei Dou, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Insect Science, Volume 27, Issue 5, Page 845-845, October 2020. (Source: Insect Science)
Source: Insect Science - August 26, 2020 Category: Biology Tags: ISSUE INFORMATION Source Type: research

Cover Caption
Spider mites are notorious agricultural pests worldwide. A male adult ofTetranychus truncatus is waiting for mating with a female. Spider mites harbor diverse bacterial communities, whose structure could be affected by many factors. We found that host species is the primary determinant of the microbiome composition of spider mites. Also, the microbiome of spider mite feces potentially reflects the gut bacterial community in spider mites (see pages 859 ‐868). Photo provided by Xiao‐Yue Hong of Nanjing Agricultural University, China. (Source: Insect Science)
Source: Insect Science - August 26, 2020 Category: Biology Tags: CONTENTS Source Type: research

Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC –MS/MS analyses
Graphical AbstractThe gene/protein repertoires ofBemisia tabaci salivary glands and secreted saliva were characterized by transcriptomic and LC –MS/MS analyses. A total of 698 salivary gland‐enriched unigenes and 171 salivary proteins were identified. AbstractThe whiteflyBemisia tabaci is a notorious agricultural pest of many crops worldwide. Although it is thought thatB. tabaci secretes saliva into the host plant to counter plant defenses, knowledge on the whitefly salivary proteome is limited. Here, we characterized the gene/protein repertoires ofB. tabaci salivary glands and secreted saliva by transcriptomic and...
Source: Insect Science - August 25, 2020 Category: Biology Authors: Hai ‐Jian Huang, Zhuang‐Xin Ye, Gang Lu, Chuan‐Xi Zhang, Jian‐Ping Chen, Jun‐Min Li Tags: ORIGINAL ARTICLE Source Type: research

Involvement of chemosensory proteins in host plant searching in the bird cherry ‐oat aphid
In this study, we present evidence for the involvement of chemosensory proteins in host plants searching of Rhopalosiphum padi. Knockdown of CSP4, CSP5 or CSP6 can significantly affect host ‐location behavior of the aphid. Host plant volatiles (octanal, [E] ‐2‐hexenol, and linalool) have significant attraction to winged adults of the species; knockdown of CSP4 or CSP5 can significantly decrease the attraction of octanal to winged adults. AbstractChemosensory systems are considered to play an important role in host plant selection in herbivorous insects. However, few studies have focused on chemosensory proteins (CSPs...
Source: Insect Science - August 24, 2020 Category: Biology Authors: Xiong Peng, Lang Liu, Yi ‐Xiao Huang, Su‐Ji Wang, De‐Xian Li, Shu‐Ting Chen, Jean‐Christophe Simon, Ming‐Jing Qu, Mao‐Hua Chen Tags: ORIGINAL ARTICLE Source Type: research

Novel alternative splicing of GABA receptor RDL exon 9 from Laodelphax striatellus modulates agonist potency
AbstractThe resistance to dieldrin gene (RDL) encodes the primary subunit of the insect ionotropicγ‐aminobutyric acid (GABA) receptor (GABAR), which is the target of phenylpyrazole and isoxazoline insecticides. The splice variants in exons 3 and 6 ofRDL, which have been widely explored in many insects, modulate the agonist potency of the homomeric RDL GABAR and potentially play an important role in the development of insects. In the present study, four splice variants of exon 9 were identified inRDL of the small brown planthopper,Laodelphax striatellus (LsRDL), resulting in LsRDL‐9a, LsRDL‐9a’, LsRDL‐9b...
Source: Insect Science - August 23, 2020 Category: Biology Authors: Jie Jiang, Li ‐Xin Huang, Feng Chen, Cheng‐Wang Sheng, Qiu‐Tang Huang, Zhao‐Jun Han, Chun‐Qing Zhao Tags: ORIGINAL ARTICLE Source Type: research

Expression and functional characterization of odorant ‐binding protein genes in the endoparasitic wasp Cotesia vestalis
Graphical AbstractWe discovered the presence of 20 OBPs in the parasitic wasp,Cotesia vestalis. RNA interference successfully inhibited the mRNA expression of CvesOBP17/18/19, and in vivo behavioral tests showed that the OBP ‐deficient wasp lost some of their ability to locate host plants and hosts. In summary, our results demonstrated that CvesOBP17/18/19 are likely involved in parasitic processes of female wasps. AbstractOdorant ‐binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory recep...
Source: Insect Science - August 23, 2020 Category: Biology Authors: Yue ‐Nan Zhou, Shuang Xie, Jia‐Ni Chen, Ze‐Hua Wang, Pei Yang, Si‐Cong Zhou, Lan Pang, Fei Li, Min Shi, Jian‐Hua Huang, Xue‐Xin Chen Tags: ORIGINAL ARTICLE Source Type: research

Phospholipase C gamma (PLC γ) regulates soluble trehalase in the 20E‐induced fecundity of Apolygus lucorum
In this study, 20E increasedAlPLC γ transcription as well as the abundance and activity of the encoded protein during molting and metamorphosis. The 20E treatment also induced the considerable accumulation of two second messengers, inositol triphosphate and diacylglycerol. The expression levels of genes encoding vitellogenin (AlVg) and soluble trehalase (AlTre ‐1) were similar to those ofAlPLC γ, and were upregulated in response to 20E. The silencing ofAlPLC γ resulted in downregulated expression ofAlTre ‐1 andAlVg. However, the silencing ofAlTre ‐1 andAlVg did not affectAlPLC γ expression. Mo...
Source: Insect Science - August 23, 2020 Category: Biology Authors: Yong ‐An Tan, Xu‐Dong Zhao, Hou‐Jun Sun, Jing Zhao, Liu‐Bin Xiao, De‐Jun Hao, Yi‐Pin Jiang Tags: ORIGINAL ARTICLE Source Type: research

Expression and functional characterization of odorant ‐binding protein genes in the endoparasitic wasp Cotesia vestalis
Graphical AbstractWe discovered the presence of 20 OBPs in the parasitic wasp,Cotesia vestalis. RNA interference successfully inhibited the mRNA expression of CvesOBP17/18/19, and in vivo behavioral tests showed that the OBP ‐deficient wasp lost some of their ability to locate host plants and hosts. In summary, our results demonstrated that CvesOBP17/18/19 are likely involved in parasitic processes of female wasps. AbstractOdorant ‐binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory recep...
Source: Insect Science - August 23, 2020 Category: Biology Authors: Yue ‐Nan Zhou, Shuang Xie, Jia‐Ni Chen, Ze‐Hua Wang, Pei Yang, Si‐Cong Zhou, Lan Pang, Fei Li, Min Shi, Jian‐Hua Huang, Xue‐Xin Chen Tags: ORIGINAL ARTICLE Source Type: research

CRISPR/Cas9 ‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori
This article is protected by copyright. All rights reserved (Source: Insect Science)
Source: Insect Science - August 22, 2020 Category: Biology Authors: Nartey Moses Addo, Xia Sun, Sheng Qin, Cheng ‐Xiang Hou, Mu‐Wang Li Tags: ORIGINAL ARTICLE Source Type: research

Susceptibility and interactions between Aedes mosquitoes and Zika viruses
In this study, we chose two Zika virus strains (FSS13025 and PRVABC59) with different abilities to infect the primary vector mosquitoesAe. aegypti andAe. albopictus. The transcriptomes and small RNA profiles of infected and uninfected mosquitoes were comparatively analyzed, and differentially expressed genes were functionally examined using RNA interference. According to the results, the susceptibility of PRVABC59 was higher than that of FSS13025 inAedes vector mosquitoes, andAe. aegypti was more susceptible to Zika virus than wasAe. albopictus. For PRVABC59 infection, specific differential expression profiles correlated w...
Source: Insect Science - August 20, 2020 Category: Biology Authors: Teng ‐Fei Zhou, Ze‐Tian Lai, Shuang Liu, Jia‐Yong Zhou, Yang Liu, Yang Wu, Ye Xu, Kun Wu, Jin‐Bao Gu, Gong Cheng, Xiao‐Guang Chen Tags: ORIGINAL ARTICLE Source Type: research

Transcription factor CncC potentially regulates the expression of multiple detoxification genes that mediate indoxacarb resistance in Spodoptera litura
This study indicates that the transcription factorSlituCncC may regulate multiple detoxification genes that mediate indoxacarb resistance inS. litura. (Source: Insect Science)
Source: Insect Science - August 20, 2020 Category: Biology Authors: Li Shi, Yao Shi, Meng ‐Fei Liu, Ya Zhang, Xiao‐Lan Liao Tags: ORIGINAL ARTICLE Source Type: research

Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes
Two cell sublines (Gr and Pl) possess the morphological characteristics of granulocytes and plasmatocytes respectively were established, and they showed different behaviors in cellular immune reactions. Two Gr and three Pl specifically expressed genes were identified by comparative analysis of their transcriptomes and RT ‐PCR and qPCR. AbstractGranulocytes and plasmatocytes play important roles in clearing foreign objects in insects, but it is difficult to distinguish between them in immune reactions. Based on the hemocyte cell line SYSU ‐OfHe ‐C established at our lab, two cell sublines, SYSU‐OfHe ‐C Granulocyte...
Source: Insect Science - August 18, 2020 Category: Biology Authors: Jian Hu, Yan Du, Meng Meng, Yipei Dong, Jiewen Peng Tags: ORIGINAL ARTICLE Source Type: research

Steroid hormone 20 ‐hydroxyecdysone promotes CTL1‐mediated cellular immunity in Helicoverpa armigera
A proposed model for the induction of HaCTL1 upon 20E signaling, thereby promoting cellular immunity. 20E ‐HaEcR‐HaUSP complex induces the expression ofHaCTL1 in hemocytes, followed by the secretion of HaCTL1 into the hemocoel. HaCTL1 then recognizes pathogens (such as parasitic nematodes and bacteria) and interacts with the unknown hemocytic receptor, which then activates cellular immune reactions such as encapsulation and phagocytosis. AbstractMermithid nematodes, such asOvomermis sinensis, are used as biological control agents against many insect pests, including cotton bollworm (Helicoverpa armigera). However, give...
Source: Insect Science - August 18, 2020 Category: Biology Authors: Gui ‐Jie Wang, Wen‐Wen Wang, Yu Liu, Lian‐Qin Chai, Guo‐Xiu Wang, Xu‐Sheng Liu, Yu‐Feng Wang, Jia‐Lin Wang Tags: ORIGINAL ARTICLE Source Type: research

Can the growing of transgenic maize threaten protected Lepidoptera in Europe?
We evaluated whether protected European butterflies can potentially be at risk if transgenic maize is extensively grown in Central Europe. We explored potential consequences of both insect resistant (IR) and herbicide resistant (HR) transgenic maize. IR maize can produce pollen that is toxic to lepidopteran larvae, and this puts butterfly species at possible risk if the presence of young larvae coincides with maize flowering, during which large quantities of maize pollen can be deposited on vegetation. By considering the timing of maize flowering in Europe, and the phenology of the protected Lepidoptera species, we found t...
Source: Insect Science - August 17, 2020 Category: Biology Authors: G ábor L. Lövei, Andreas Lang, Marco Ferrante, Victor Bacle Tags: ORIGINAL ARTICLE Source Type: research