Compartmentalized muscle redox signals controlling exercise metabolism – Current state, future challenges
Publication date: Available online 22 February 2020Source: Redox BiologyAuthor(s): Carlos Henriquez-Olguin, Roberto Meneses-Valdes, Thomas E. JensenAbstractExercise imposes cellular stress on contracting skeletal muscle fibers, forcing them to complete molecular adaptations to maintain homeostasis. There is mounting evidence that redox signaling by reactive oxygen species (ROS) is vital for skeletal muscle exercise adaptations across many different exercise modalities. The study of redox signaling is moving towards a growing appreciation that these ROS do not signal in a global unspecific way, but rather elicit their effec...
Source: Redox Biology - February 23, 2020 Category: Biology Source Type: research

Antioxidant supplements and endurance exercise: Current evidence and mechanistic insights
Publication date: Available online 20 February 2020Source: Redox BiologyAuthor(s): Shaun A. Mason, Adam J. Trewin, Lewan Parker, Glenn D. WadleyAbstractAntioxidant supplements are commonly consumed by endurance athletes to minimize exercise-induced oxidative stress, with the intention of enhancing recovery and improving performance. There are numerous commercially available nutritional supplements that are targeted to athletes and health enthusiasts that allegedly possess antioxidant properties. However, most of these compounds are poorly investigated with respect to their in vivo redox activity and efficacy in humans. The...
Source: Redox Biology - February 21, 2020 Category: Biology Source Type: research

End-stage renal disease is different from chronic kidney disease in upregulating ROS-modulated proinflammatory secretome in PBMCs - A novel multiple-hit model for disease progression
ConclusionsOur analysis suggests novel secretomic upregulation in PBMCs of patients with CKD and ESRD, act synergistically with uremic toxins, to promote inflammation and potential disease progression. Our findings have provided novel insights on PBMC secretome upregulation to promote disease progression and may lead to the identification of new therapeutic targets for novel regimens for CKD, ESRD and their accelerated cardiovascular disease, other inflammations and cancers. (Total words: 279). (Source: Redox Biology)
Source: Redox Biology - February 21, 2020 Category: Biology Source Type: research

CO-mediated cytoprotection is dependent on cell metabolism modulation
Publication date: Available online 19 February 2020Source: Redox BiologyAuthor(s): Cláudia Figueiredo-Pereira, Daniela Dias-Pedroso, Nuno L. Soares, Helena L.A. VieiraAbstractCarbon monoxide (CO) is a gasotransmitter endogenously produced by the activity of heme oxygenase, which is a stress-response enzyme. Endogenous CO or low concentrations of exogenous CO have been described to present several cytoprotective functions: anti-apoptosis, anti-inflammatory, vasomodulation, maintenance of homeostasis, stimulation of preconditioning and modulation of cell differentiation. The present review revises and discuss how CO r...
Source: Redox Biology - February 20, 2020 Category: Biology Source Type: research

The systemic role of SIRT1 in exercise mediated adaptation
Publication date: Available online 14 February 2020Source: Redox BiologyAuthor(s): Zsolt Radak, Katsuhiko Suzuki, Aniko Posa, Zita Petrovszky, Erika Koltai, Istvan BoldoghAbstractCellular energy demands are readily changed during physical exercise resulting in adaptive responses by signaling proteins of metabolic processes, including the NAD+ dependent lysine deacetylase SIRT1. Regular exercise results in systemic adaptation that restores the level of SIRT1 in the kidney, liver, and brain in patients with neurodegenerative diseases, and thereby normalizes cellular metabolic processes to attenuate the severity of these dise...
Source: Redox Biology - February 16, 2020 Category: Biology Source Type: research

Emerging perspectives on cytoglobin, beyond NO dioxygenase and peroxidase
Publication date: Available online 14 February 2020Source: Redox BiologyAuthor(s): Clinton Mathai, Frances L. Jourd'heuil, Reynold I. Lopez-Soler, David Jourd'heuilAbstractCytoglobin is an evolutionary ancient hemoglobin with poor functional annotation. Rather than constrained to penta coordination, cytoglobin's heme iron may exist either as a penta or hexacoordinated arrangement when exposed to different intracellular environments. Two cysteine residues at the surface of the protein form an intramolecular disulfide bond that regulates iron coordination, ligand binding, and peroxidase activity. Overall, biochemical results...
Source: Redox Biology - February 16, 2020 Category: Biology Source Type: research

A closer look into NADPH oxidase inhibitors: Validation and insight into their mechanism of action
Publication date: Available online 15 February 2020Source: Redox BiologyAuthor(s): Joana Reis, Marta Massari, Sara Marchese, Marta Ceccon, Friso S. Aalbers, Federica Corana, Sergio Valente, Antonello Mai, Francesca Magnani, Andrea MatteviAbstractNADPH-oxidases (NOXs) purposefully produce reactive-oxygen-species (ROS) and are found in most kingdoms of life. The seven human NOXs are each characterized by a specific expression profile and a fine regulation to spatio-temporally tune ROS concentration in cells and tissues. One of the best characterized roles for NOXs is in host protection against pathogens but ROS themselves ar...
Source: Redox Biology - February 16, 2020 Category: Biology Source Type: research

Plasma carotenoids, tocopherols and retinol - Association with age in the Berlin Aging Study II
Publication date: Available online 13 February 2020Source: Redox BiologyAuthor(s): Daniela Weber, Bastian Kochlik, Ilja Demuth, Elisabeth Steinhagen-Thiessen, Tilman Grune, Kristina NormanAbstractRegular consumption of fruits and vegetables, which is related to high plasma levels of lipid-soluble micronutrients such as carotenoids and tocopherols, is linked to lower incidences of various age-related diseases. Differences in lipid-soluble micronutrient blood concentrations seem to be associated with age. Our retrospective analysis included men and women aged 22–37 and 60–85 years from the Berlin Aging Study II. ...
Source: Redox Biology - February 14, 2020 Category: Biology Source Type: research

Nitrite elicits divergent NO-dependent signaling that associates with outcome in out of hospital cardiac arrest
Publication date: Available online 14 February 2020Source: Redox BiologyAuthor(s): Dario A. Vitturi, Charles Maynard, Michele Olsufka, Adam C. Straub, Nick Krehel, Peter J. Kudenchuk, Graham Nichol, Michael Sayre, Francis Kim, Cameron DezfulianAbstractBrain and heart injury cause most out-of-hospital cardiac arrest deaths but limited pharmacotherapy exists to protect these tissues. Nitrite is a nitric oxide precursor that is protective in pre-clinical models of ischemic injury and safe in Phase I testing. Protection may occur by cGMP generation via the sGC pathway or through S-nitrosothiol and nitrated conjugated linoleic ...
Source: Redox Biology - February 14, 2020 Category: Biology Source Type: research

Mechanisms of exercise-induced preconditioning in skeletal muscles
Publication date: Available online 12 February 2020Source: Redox BiologyAuthor(s): Scott K. Powers, Matthew Bomkamp, Mustafa Ozdemir, Hayden HyattAbstractEndurance exercise training promotes numerous biochemical adaptations within skeletal muscle fibers culminating into a phenotype that is safeguarded against numerous perils including doxorubicin-induced myopathy and inactivity-induced muscle atrophy. This exercise-induced protection of skeletal muscle fibers is commonly termed “exercise preconditioning”. This review will discuss the biochemical mechanisms responsible for exercise-induced protection of skeletal...
Source: Redox Biology - February 13, 2020 Category: Biology Source Type: research

Molecular pathways driving omeprazole nephrotoxicity
In conclusion, omeprazole nephrotoxicity may be related to induction of oxidative stress and renal tubular cell death. (Source: Redox Biology)
Source: Redox Biology - February 13, 2020 Category: Biology Source Type: research

Waterpipe (shisha, hookah) smoking, oxidative stress and hidden disease potential
Publication date: Available online 11 February 2020Source: Redox BiologyAuthor(s): Mohammad Badran, Ismail LaherAbstractDue to the abundant research on the harmful effects of cigarette smoking and the strict regulations enacted by many health authorities, many smokers are seeking for safer and more acceptable tobacco forms. Waterpipe (also known as shisha or hookah) use has increased dramatically during the past decade, mostly due to its improved taste, lack of regulations and social acceptability as a safer option than cigarettes. However, recent clinical and experimental studies indicate that waterpipe use is as, or even...
Source: Redox Biology - February 13, 2020 Category: Biology Source Type: research

Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery
Publication date: Available online 11 February 2020Source: Redox BiologyAuthor(s): Stamatia Laidou, Gregorio Alanis-Lobato, Jan Pribyl, Tamás Raskó, Boris Tichy, Kamil Mikulasek, Maria Tsagiopoulou, Jan Oppelt, Georgia Kastrinaki, Maria Lefaki, Manvendra Singh, Annika Zink, Niki Chondrogianni, Fotis Psomopoulos, Alessandro Prigione, Zoltan Ivics, Sarka Pospisilova, Petr Skladal, Zsuzsanna Izsvák, Miguel A. Andrade-NavarroAbstractSpinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and se...
Source: Redox Biology - February 13, 2020 Category: Biology Source Type: research

CircRNA_0001449 disturbs phosphatidylinositol homeostasis and AKT activity by enhancing Osbpl5 translation in transient cerebral ischemia
Publication date: Available online 10 February 2020Source: Redox BiologyAuthor(s): Fei-Fei Shang, Li Luo, Jianghong Yan, Qiubo Yu, Yongzheng Guo, Yuchen Wen, Xiao-Li Min, Ling Jiang, Xiang He, Wei LiuAbstractPhosphatidylinositol-3,4,5-trisphosphate [PI(3,4,5)P3] is a phosphorylated derivative of phosphatidylinositol 4-phosphate [PI(4)P] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which recruit and activate AKT in the plasma membrane (PM) to promote cellular survival. ORP5 anchors at the endoplasmic reticulum (ER)-PM contact sites and acts as a PI(4)P and PI(4,5)P2/phosphatidylserine (PS) exchanger. Here, a lipid...
Source: Redox Biology - February 11, 2020 Category: Biology Source Type: research

Lectin-induced oxidative stress in human platelets
In conclusion lectins, especially WGA, induce oxidative stress in platelets and decrease energy availability through modifications of membrane structure leading to the inefficiency of the aerobic machinery that steers platelets toward death as suggested by the decreased metabolic activity of platelets and the increased lactic dehydrogenase release. (Source: Redox Biology)
Source: Redox Biology - February 10, 2020 Category: Biology Source Type: research

Lactate as a fulcrum of metabolism
Publication date: Available online 9 February 2020Source: Redox BiologyAuthor(s): George A. BrooksAbstractMistakenly thought to be the consequence of oxygen lack in contracting skeletal muscle we now know that the l-enantiomer of the lactate anion is formed under fully aerobic conditions and is utilized continuously in diverse cells, tissues, organs and at the whole-body level. By shuttling between producer (driver) and consumer (recipient) cells lactate fulfills at least three purposes: 1] a major energy source for mitochondrial respiration; 2] the major gluconeogenic precursor; and 3] a signaling molecule. Working by mas...
Source: Redox Biology - February 10, 2020 Category: Biology Source Type: research

Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
Publication date: Available online 7 February 2020Source: Redox BiologyAuthor(s): Ana Isabel Santos, Ana Sofia Lourenço, Sónia Simão, Dorinda Marques da Silva, Daniela Filipa Santos, Ana P. Onofre de Carvalho, Ana Catarina Pereira, Alicia Izquierdo-Álvarez, Elena Ramos, Esperanza Morato, Anabel Marina, Antonio Martínez-Ruiz, Inês M. AraújoAbstractNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the...
Source: Redox Biology - February 8, 2020 Category: Biology Source Type: research

Redox-dependent regulation of mitochondrial dynamics by DJ-1 paralogs in Saccharomyces cerevisiae
Publication date: Available online 7 February 2020Source: Redox BiologyAuthor(s): Kondalarao Bankapalli, Vinaya Vishwanathan, Gautam Susarla, N. Sunayana, SreeDivya Saladi, Divya Peethambaram, Patrick D'SilvaAbstractMitochondria are indispensable organelles that perform critical cellular functions, including energy metabolism, neurotransmission, and synaptic maintenance. Mitochondrial dysfunction and impairment in the organellar homeostasis are key hallmarks implicated in the progression of neurodegenerative disorders. The members of DJ-1/ThiJ/PfpI family are highly conserved, and loss of DJ-1 (PARK7) function in humans re...
Source: Redox Biology - February 8, 2020 Category: Biology Source Type: research

Mitochondrial complex I derived ROS regulate stress adaptation in Drosophila melanogaster
Publication date: Available online 7 February 2020Source: Redox BiologyAuthor(s): Filippo Scialo, Ashwin Sriram, Rhoda Stefanatos, Ruth V. Spriggs, Samantha H.Y. Loh, L. Miguel Martins, Alberto SanzAbstractReactive Oxygen Species (ROS) are essential cellular messengers required for cellular homeostasis and regulate the lifespan of several animal species. The main site of ROS production is the mitochondrion, and within it, respiratory complex I (CI) is the main ROS generator. ROS produced by CI trigger several physiological responses that are essential for the survival of neurons, cardiomyocytes or macrophages. Here, we sho...
Source: Redox Biology - February 8, 2020 Category: Biology Source Type: research

Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
Publication date: Available online 6 February 2020Source: Redox BiologyAuthor(s): Shan Deng, Kobina Essandoh, Xiaohong Wang, Yutian Li, Wei Huang, Jing Chen, Jiangtong Peng, Ding-Sheng Jiang, Xingjiang Mu, Chenran Wang, Tianqing Peng, Jun-Lin Guan, Yigang Wang, Anil Jegga, Kai Huang, Guo-Chang FanAbstractCurrently, most antioxidants do not show any favorable clinical outcomes in reducing myocardial ischemia-reperfusion (I/R) injury, suggesting an urgent need for exploring a new regulator of redox homeostasis in I/R hearts. Here, using heart-specific transgenic (TG) and knockdown (KD) mouse models, tumor susceptibility gene...
Source: Redox Biology - February 7, 2020 Category: Biology Source Type: research

Redox-mediated regulation of aging and healthspan by an evolutionarily conserved transcription factor HLH-2/Tcf3/E2A
Publication date: Available online 4 February 2020Source: Redox BiologyAuthor(s): Leonid Rozanov, Meenakshi Ravichandran, Giovanna Grigolon, Maria-Clara Zanellati, Johannes Mansfeld, Kim Zarse, Nir Barzilai, Gil Atzmon, Fabian Fischer, Michael RistowAbstractPhysiological aging is a complex process, influenced by a plethora of genetic and environmental factors. While being far from fully understood, a number of common aging hallmarks have been elucidated in recent years. Among these, transcriptomic alterations are hypothesized to represent a crucial early manifestation of aging. Accordingly, several transcription factors (T...
Source: Redox Biology - February 6, 2020 Category: Biology Source Type: research

Ginsenoside Rh2 pretreatment and withdrawal reactivated the pentose phosphate pathway to ameliorate intracellular redox disturbance and promoted intratumoral penetration of adriamycin
Publication date: Available online 5 February 2020Source: Redox BiologyAuthor(s): Jiali Liu, Qingyun Cai, Wenjie Wang, Meng Lu, JianMing Liu, Fang Zhou, Minjie Sun, Guangji Wang, Jingwei ZhangAbstractImproving the limited penetration, accumulation and therapeutic effects of antitumor drugs in the avascular region of the tumor mass is crucial during chemotherapy. P-gp inhibitors have achieved little success despite significant efforts. Excessive P-gp inhibition disturbed the kinetic balance between intracellular accumulation and intercellular penetration, thus resulting in a more inhomogeneous distribution of substrate drug...
Source: Redox Biology - February 6, 2020 Category: Biology Source Type: research

Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different Aβ concentrations
Publication date: Available online 31 January 2020Source: Redox BiologyAuthor(s): Kim Cuong Cap, Yeon-Joo Jung, Bo Young Choi, Seung Jae Hyeon, Jae-Gyu Kim, Jung-Ki Min, Rokibul Islam, Abu Jubayer Hossain, Won-Suk Chung, Sang Won Suh, Hoon Ryu, Jae-Bong ParkAbstractBoth the accumulation of Amyloid-β (Aβ) in plaques and phosphorylation of Tau protein (p-Tau) in neurofibrillary tangles have been identified as two major symptomatic features of Alzheimer's disease (AD). Despite of critical role of Aβ and p-Tau in AD progress, the interconnection of signalling pathways that Aβ induces p-Tau remains elusive. ...
Source: Redox Biology - February 2, 2020 Category: Biology Source Type: research

DNA hypermethylation: A novel mechanism of CREG gene suppression and atherosclerogenic endothelial dysfunction
ConclusionsOur study provides the first direct evidence that DNMT3B-mediated CREG gene hypermethylation is a novel mechanism that contributes to endothelial dysfunction and atherosclerosis development. Blocking CREG methylation may represent a novel therapeutic approach to treat ox-LDL-induced atherosclerosis. (Source: Redox Biology)
Source: Redox Biology - February 2, 2020 Category: Biology Source Type: research

Environmental risk factors and their footprints in vivo – A proposal for the classification of oxidative stress biomarkers
Publication date: Available online 31 January 2020Source: Redox BiologyAuthor(s): Pietro GhezziAbstractEnvironmental agents, including socioeconomic condition, and host factors can act as causal agents and risk factors in disease. We use biomarkers and sociomarkers to study causal factors, such as overproduction of reactive oxygen species (ROS) which could play a role in disease through oxidative stress. It is therefore important to define the exact meaning of the biomarker we measure. In this review we attempt a classification of biomarkers related to oxidative stress based on their biological meaning. We define as type z...
Source: Redox Biology - February 1, 2020 Category: Biology Source Type: research

Hydrogen sulfide stimulates xanthine oxidoreductase conversion to nitrite reductase and formation of NO
Publication date: Available online 30 January 2020Source: Redox BiologyAuthor(s): Sibile Pardue, Gopi K. Kolluru, Xinggui Shen, Sara E. Lewis, Courtney B. Saffle, Eric E. Kelley, Christopher G. KevilAbstractCardiovascular disease is the leading cause of death and disability worldwide with increased oxidative stress, and reduced NO bioavailability serving as a key risk factors. For decades, elevation in protein abundance and enzymatic activity of xanthine oxidoreductase (XOR) under hypoxic/inflammatory conditions has been associated with organ damage and vascular dysfunction. Recent reports have challenged this dogma by ide...
Source: Redox Biology - January 30, 2020 Category: Biology Source Type: research

A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38
Publication date: Available online 28 January 2020Source: Redox BiologyAuthor(s): Chuanbin Yang, Zhou Zhu, Benjamin Chun-Kit Tong, Ashok Iyaswamy, Wen-Jian Xie, Yu Zhu, Sravan Gopalkrishnashetty Sreenivasmurthy, Krishnamoorthi Senthilkumar, King-Ho Cheung, Ju-Xian Song, Hong-Jie Zhang, Min LiAbstractTFEB (transcription factor EB) and TFE3 (transcription factor E3) are “master regulators” of autophagy and lysosomal biogenesis. The stress response p38 mitogen-activated protein (MAP) kinases affects multiple intracellular responses including inflammation, cell growth, differentiation, cell death, senescence, tumor...
Source: Redox Biology - January 29, 2020 Category: Biology Source Type: research

An emerging perspective on sex differences: Intersecting S-nitrosothiol and aldehyde signaling in the heart
Publication date: Available online 25 January 2020Source: Redox BiologyAuthor(s): Kevin M. Casin, Mark J. KohrAbstractCardiovascular disease is the leading cause of the death for both men and women. Although baseline heart physiology and the response to disease are known to differ by sex, little is known about sex differences in baseline molecular signaling, especially with regard to redox biology. In this review, we describe current research on sex differences in cardiac redox biology with a focus on the regulation of nitric oxide and aldehyde signaling. Furthermore, we argue for a new perspective on cardiovascular sex di...
Source: Redox Biology - January 26, 2020 Category: Biology Source Type: research

Cellular stress responses and dysfunctional Mitochondrial–cellular senescence, and therapeutics in chronic respiratory diseases
Publication date: Available online 25 January 2020Source: Redox BiologyAuthor(s): Marko Manevski, Thivanka Muthumalage, Dinesh Devadoss, Isaac K. Sundar, Qixin Wang, Kameshwar P. Singh, Hoshang J. Unwalla, Hitendra S. Chand, Irfan RahmanAbstractThe abnormal inflammatory responses due to the lung tissue damage and ineffective repair/resolution in response to the inhaled toxicants result in the pathological changes associated with chronic respiratory diseases. Investigation of such pathophysiological mechanisms provides the opportunity to develop the molecular phenotype-specific diagnostic assays and could help in designing ...
Source: Redox Biology - January 26, 2020 Category: Biology Source Type: research

Redox requirements for ubiquitin-like urmylation of Ahp1, a 2-Cys peroxiredoxin from yeast
Publication date: Available online 22 January 2020Source: Redox BiologyAuthor(s): Cindy Brachmann, Lars Kaduhr, André Jüdes, Keerthiraju Ethiraju Ravichandran, James D. West, Sebastian Glatt, Raffael SchaffrathAbstractThe yeast peroxiredoxin Ahp1, like related anti-oxidant enzymes in other species, undergoes urmylation, a lysine-directed conjugation to ubiquitin-like modifier Urm1. Ahp1 assembles into a homodimer that detoxifies peroxides via forming intersubunit disulfides between peroxidatic and resolving cysteines that are subsequently reduced by the thioredoxin system. Although urmylation coincides with oxi...
Source: Redox Biology - January 23, 2020 Category: Biology Source Type: research

Inhibition of TXNRD or SOD1 overcomes NRF2-mediated resistance to β-lapachone
Publication date: Available online 23 January 2020Source: Redox BiologyAuthor(s): Laura Torrente, Nicolas Prieto Farigua, Aimee Falzone, Cody M. Elkins, David A. Boothman, Eric B. Haura, Gina M. DeNicolaAbstractAlterations in the NRF2/KEAP1 pathway result in the constitutive activation of NRF2, leading to the aberrant induction of antioxidant and detoxification enzymes, including NQO1. The NQO1 bioactivatable agent β-lapachone can target cells with high NQO1 expression but relies in the generation of reactive oxygen species (ROS), which are actively scavenged in cells with NRF2/KEAP1 mutations. However, whether NRF2/K...
Source: Redox Biology - January 23, 2020 Category: Biology Source Type: research

Circulating biomarkers of antioxidant status and oxidative stress in people with cystic fibrosis: A systematic review and meta-analysis
ConclusionsThis systematic review and meta-analysis found good evidence for reduced antioxidant capacity and elevated oxidative stress in people with clinically-stable CF.Graphical abstract (Source: Redox Biology)
Source: Redox Biology - January 23, 2020 Category: Biology Source Type: research

Estrogenic control of mitochondrial function
Publication date: Available online 23 January 2020Source: Redox BiologyAuthor(s): Carolyn M. KlingeAbstractSex-based differences in human disease are caused in part by the levels of endogenous sex steroid hormones which regulate mitochondrial metabolism. This review updates a previous review on how estrogens regulate metabolism and mitochondrial function that was published in 2017. Estrogens are produced by ovaries and adrenals, and in lesser amounts by adipose, breast stromal, and brain tissues. At the cellular level, the mechanisms by which estrogens regulate diverse cellular functions including reproduction and behavior...
Source: Redox Biology - January 23, 2020 Category: Biology Source Type: research

Comparative analysis of the mitochondrial morphology, energy metabolism, and gene expression signatures in three types of blastocyst-derived stem cells
In this study, we focused on characterizing the three stem cell types derived from mouse blastocysts by observing cellular organelles, especially the mitochondria, and analyzing how mitochondrial dynamics relates to the energy metabolism in each cell type. Our study revealed that XEN cells have distinct mitochondrial morphology and energy metabolism compared with that in ESCs and TSCs. In addition, by analyzing the energy metabolism (oxygen consumption and extracellular acidification rates), we demonstrated that differences in the mitochondria affect the cellular metabolism in the stem cells. RNA sequencing analysis showed...
Source: Redox Biology - January 21, 2020 Category: Biology Source Type: research

Effect of ER stress on sphingolipid levels and apoptotic pathways in retinal pigment epithelial cells
ConclusionsThe results of this study reveal the presence of increased long chain CERs, C1P and apoptotic markers in retinal cells undergoing ER stress. (Source: Redox Biology)
Source: Redox Biology - January 20, 2020 Category: Biology Source Type: research

Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation
In conclusion, our study has established a pathophysiological role for ROS signaling in atrial cardiomyocytes, and it may be that ox-CaMKII and p-CaMKII (Thr-286) are activated by ROS to increase AF susceptibility following ibrutinib treatment. We have also identified the inhibition of NOX as a potential novel AF therapy approach. (Source: Redox Biology)
Source: Redox Biology - January 20, 2020 Category: Biology Source Type: research

Redox activation of excitatory pathways in auditory neurons as mechanism of age-related hearing loss
Publication date: Available online 20 January 2020Source: Redox BiologyAuthor(s): Francis Rousset, German Nacher-Soler, Marta Coelho, Sten Ilmjarv, Vivianne Beatrix Christina Kokje, Antoine Marteyn, Yves Cambet, Michael Perny, Marta Roccio, Vincent Jaquet, Pascal Senn, Karl Heinz KrauseAbstractAge-related hearing (ARHL) loss affects a large part of the human population with a major impact on our aging societies. Yet, underlying mechanisms are not understood, and no validated therapy or prevention exists. NADPH oxidases (NOX), are important sources of reactive oxygen species (ROS) in the cochlea and might therefore be invol...
Source: Redox Biology - January 20, 2020 Category: Biology Source Type: research

Exercise shapes redox signaling in cancer
Publication date: Available online 18 January 2020Source: Redox BiologyAuthor(s): Mohamad Assi, Suzanne Dufresne, Amélie RébillardAbstractIn this paper of the special issue dedicated for the Olympics 2020, we put the light on an exciting facet of exercise-oncology, which may still be unknown to some audience. Accumulating convincing evidences show that exercise reduces cancer progression and recurrence mainly in colon and breast cancer patients. Interestingly, the positive effects of exercise on cancer outcomes were mainly observed when patients practiced vigorous exercise of 6 METs or more. At the molecular ...
Source: Redox Biology - January 19, 2020 Category: Biology Source Type: research

Indoxyl sulfate impairs valsartan-induced neovascularization
Publication date: Available online 14 January 2020Source: Redox BiologyAuthor(s): Ko-Lin Kuo, Jin-Feng Zhao, Po-Hsun Huang, Bei-Chia Guo, Der-Cherng Tarng, Tzong-Shyuan LeeAbstractStudies revealed that the use of renin-angiotensin-aldosterone system antagonism is not associated with a statistically significant reduction in the risk of cardiovascular events in patients with chronic kidney disease (CKD) compared with that in the general population. We tested the hypothesis that indoxyl sulfate (IS) can interfere with the protective effect of valsartan-mediated on endothelial function in vitro and neovascularization in mice u...
Source: Redox Biology - January 15, 2020 Category: Biology Source Type: research

Pro-survival and anti-inflammatory roles of NF-κB c-Rel in the Parkinson's disease models
Publication date: Available online 13 January 2020Source: Redox BiologyAuthor(s): Zishan Wang, Hongtian Dong, Jinghui Wang, Yulu Huang, Xiaoshuang Zhang, Yilin Tang, Qing Li, Zhaolin Liu, Yuanyuan Ma, Jiabin Tong, Li Huang, Jian Fei, Mei Yu, Jian Wang, Fang HuangAbstractThe pathological hallmarks of Parkinson's disease (PD) are the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc) and the presence of overactivated glial cells and neuroinflammation. Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) c-Rel subunit is closely related in the pathological progre...
Source: Redox Biology - January 14, 2020 Category: Biology Source Type: research

Excessive oxidative stress in cumulus granulosa cells induced cell senescence contributes to endometriosis-associated infertility
In conclusion, our results indicated excessive reactive oxygen species induces senescence of endometriosis GCs via arouse ER stress, which finally contributes to endometriosis-associated infertility, and melatonin may represent a novel adjuvant therapy strategy for endometriosis-associated infertility.Graphical abstract (Source: Redox Biology)
Source: Redox Biology - January 13, 2020 Category: Biology Source Type: research

Corrigendum to “Mapping glutathione utilization in the developing zebrafish (Danio rerio) embryo” [Redox Biol. 26 (2019) 101235]
Publication date: Available online 13 January 2020Source: Redox BiologyAuthor(s): Archit Rastogi, Christopher W. Clark, Sarah M. Conlin, Sarah E. Brown, Alicia R. Timme-Laragy (Source: Redox Biology)
Source: Redox Biology - January 13, 2020 Category: Biology Source Type: research

Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes
Publication date: Available online 11 January 2020Source: Redox BiologyAuthor(s): Caitlin J. Lewis, Bhavna Dixit, Elizabeth Batiuk, Carter J. Hall, Matthew S. O'Connor, Amutha BoominathanAbstractMutations in mitochondrial DNA can be inherited or occur de novo leading to several debilitating myopathies with no curative option and few or no effective treatments. Allotopic expression of recoded mitochondrial genes from the nucleus has potential as a gene therapy strategy for such conditions, however progress in this field has been hampered by technical challenges. Here we employed codon optimization as a tool to re-engineer t...
Source: Redox Biology - January 12, 2020 Category: Biology Source Type: research

Mitochondrial quality control in pulmonary fibrosis
Publication date: Available online 8 January 2020Source: Redox BiologyAuthor(s): Jennifer L. Larson-Casey, Chao He, A.Brent CarterAbstractMechanisms underlying the pathogenesis of pulmonary fibrosis remain incompletely understood. Emerging evidence suggests changes in mitochondrial quality control are a critical determinant in many lung diseases, including chronic obstructive pulmonary disease (COPD), asthma, pulmonary hypertension, acute lung injury, lung cancer, and in the susceptibility to pulmonary fibrosis. Once thought of as the kidney-bean shaped powerhouses of the cell, mitochondria are now known to form interconne...
Source: Redox Biology - January 8, 2020 Category: Biology Source Type: research

Methylglyoxal interaction with superoxide dismutase 1
Publication date: Available online 7 January 2020Source: Redox BiologyAuthor(s): P. Polykretis, E. Luchinat, F. Boscaro, L. BanciAbstractMethylglyoxal (MG) is a highly reactive aldehyde spontaneously formed in human cells mainly as a by-product of glycolysis. Such endogenous metabolite reacts with proteins, nucleotides and lipids forming advanced glycation end-products (AGEs). MG binds to arginine, lysine and cysteine residues of proteins causing the formation of stable adducts that can interfere with protein function. Among the proteins affected by glycation, MG has been found to react with superoxide dismutase 1 (SOD1), ...
Source: Redox Biology - January 8, 2020 Category: Biology Source Type: research

Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer
We examined the role of dihydrolipoamide dehydrogenase (DLD) in ferroptosis induction in head and neck cancer (HNC). The effects of cystine deprivation or sulfasalazine treatment and of DLD gene silencing/overexpression were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed with regard to cell death, lipid reactive oxygen species (ROS) and mitochondrial iron production, mitochondrial membrane potential, mRNA/protein expression, and α-ketoglutarate dehydrogenase (KGDH)/succinate/aconitase activities. Cystine deprivation induced ferroptosis via glutaminolysis. Cystine deprivation or...
Source: Redox Biology - January 8, 2020 Category: Biology Source Type: research

xCT (SLC7A11) expression confers intrinsic resistance to physical plasma treatment in tumor cells
Publication date: Available online 3 January 2020Source: Redox BiologyAuthor(s): Sander Bekeschus, Sebastian Eisenmann, Sanjeev Kumar Sagwal, Gabriella Pasqual-Melo, Juliane Moritz, Yana Bodnar, Juliane Moritz, Broder Poschkamp, Ingo Stoffels, Steffen Emmert, Thomas von Woedtke, Madesh Muniswamy, Klaus-Dieter Weltmann, Rajesh Kumar GandhirajanAbstractCold physical plasma is a partially ionized gas investigated as a new anticancer tool in selectively targeting cancer cells in monotherapy or in combination with therapeutic agents. Here, we investigated the intrinsic resistance mechanisms of tumor cells towards physical plasm...
Source: Redox Biology - January 5, 2020 Category: Biology Source Type: research

Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury
This study aimed to identify which HECT-type E3 ubiquitin ligase is involved in endothelial injury and clarify the mechanisms at molecular, cellular, and organism levels. We revealed a novel role of the HECT-type E3 ubiquitin ligase WWP2 in regulating endothelial injury and vascular remodeling after endothelial injury. Endothelial/myeloid-specific WWP2 knockout in mice significantly aggravated angiotensin II/oxidative stress-induced endothelial injury and vascular remodeling after endothelial injury. The same results were obtained from in vitro experiments. Mechanistically, the endothelial injury factor Septin4 was identif...
Source: Redox Biology - January 3, 2020 Category: Biology Source Type: research

Transcription factor NRF2 uses the Hippo pathway effector TAZ to induce tumorigenesis in glioblastomas
In this study, we discover that NRF2 also propels tumorigenesis in gliomas and glioblastomas by inducing the expression of the transcriptional co-activator TAZ, a protein of the Hippo signaling pathway that promotes tumor growth. The expression of the genes encoding NRF2 (NFE2L2) and TAZ (WWTR1) showed a positive correlation in 721 gliomas from The Cancer Genome Atlas database. Moreover, NRF2 and TAZ protein levels also correlated in immunohistochemical tissue arrays of glioblastomas. Genetic knock-down of NRF2 decreased, while NRF2 overexpression or chemical activation with sulforaphane, increased TAZ transcript and prote...
Source: Redox Biology - January 3, 2020 Category: Biology Source Type: research

GSTpi regulates VE-cadherin stabilization through promoting S-glutathionylation of Src
This study revealed a novel regulatory effect of GSTpi on vascular endothelial barrier function and the importance of S-glutathionylation of Src induced by GSTpi in the activation of Src/VE-cadherin pathway.Graphical abstract (Source: Redox Biology)
Source: Redox Biology - January 1, 2020 Category: Biology Source Type: research