Insulin signalling elicits hunger-induced feeding in Drosophila.
Abstract Insulin, a highly conserved peptide hormone, links nutrient availability to metabolism and growth in animals. In fed states insulin levels remain high and in animals that are food deprived insulin signalling drops. Here, we report that in Drosophila, feeding elicited by short periods of starvation is dependent on insulin signalling. The activity of insulin signalling pathway in the abdominal fatbody aids in feeding during short periods of starvation. A feedback regulatory signalling that involves cells that express the Drosophila hunger hormone short-neuropeptide-F (sNPF) and insulin-producing cells susta...
Source: Developmental Biology - November 23, 2019 Category: Biology Authors: Sudhakar SR, Pathak H, Rehman N, Fernandes J, Vishnu S, Varghese J Tags: Dev Biol Source Type: research

Six2 regulates Pax9 expression, palatogenesis and craniofacial bone formation.
In this study, we investigated the role of the transcription factor Six2 in palate development. Six2 was selected using the SysFACE tool to predict genes from the 2p21 locus, a region associated with clefting in humans by GWAS, that are likely to be involved in palatogenesis. We functionally validated the predicted role of Six2 in palatogenesis by showing that 22% of Six2 null embryos develop cleft palate. Six2 contributes to palatogenesis by promoting mesenchymal cell proliferation and regulating bone formation. The clefting phenotype in Six2-/- embryos is similar to Pax9 null embryos, so we examined the functional relati...
Source: Developmental Biology - November 22, 2019 Category: Biology Authors: Sweat YY, Sweat M, Mansaray M, Cao H, Eliason S, Adeyemo WL, Gowans LJJ, Eshete MA, Anand D, Chalkley C, Saadi I, Lachke SA, Butali A, Amendt BA Tags: Dev Biol Source Type: research

Left-right asymmetric heart jogging increases the robustness of dextral heart looping in zebrafish.
Abstract Building a left-right (L-R) asymmetric organ requires asymmetric information. This comes from various sources, including asymmetries in embryo-scale genetic cascades, organ-intrinsic mechanical forces, and cell-level chirality, but the relative influence of these sources and how they collaborate to drive asymmetric morphogenesis is not understood. During zebrafish heart development, the linear heart tube extends to the left of the midline in a process known as jogging. The jogged heart then undergoes dextral (i.e. rightward) looping to correctly position the heart chambers relative to one another. Left la...
Source: Developmental Biology - November 20, 2019 Category: Biology Authors: Grimes DT, Patterson VL, Luna-Arvizu G, Schottenfeld-Roames J, Irons ZH, Burdine RD Tags: Dev Biol Source Type: research

Inositol 1,4,5-trisphosphate receptor 2 as a novel marker of vasculature to delineate processes of cardiopulmonary development.
Abstract Congenital heart diseases (CHDs) involving the outflow tract (OFT), such as persistent truncus arteriosus (PTA), lead to mortality and morbidity with implications not only in the heart, but also in the pulmonary vasculature. The mechanisms of pulmonary artery (PA) development and the etiologies underlying PA disorders associated with CHD remain poorly understood partly because of a specific marker for PA development is nonexistent. The three subtypes of inositol 1,4,5-trisphosphate receptors (IP3R1, 2, and 3) are intracellular Ca2+ channels that are essential for many tissues and organs. We discovered tha...
Source: Developmental Biology - November 20, 2019 Category: Biology Authors: Ishizaki-Asami R, Uchida K, Tsuchihashi T, Shibata A, Kodo K, Emoto K, Mikoshiba K, Takahashi T, Yamagishi H Tags: Dev Biol Source Type: research

Gata is ubiquitously required for the earliest zygotic gene transcription in the ascidian embryo.
u Y Abstract In ascidian embryos, the earliest transcription from the zygotic genome begins between the 8-cell and 16-cell stages. Gata.a, a maternally expressed Gata transcription factor, activates target genes specifically in the animal hemisphere, whereas the complex of β-catenin and Tcf7 antagonizes the activity of Gata.a and activates target genes specifically in the vegetal hemisphere. Here, we show that genes zygotically expressed at the 16-cell stage have significantly more Gata motifs in their upstream regions. These genes included not only genes with animal hemisphere-specific expression but also ge...
Source: Developmental Biology - November 18, 2019 Category: Biology Authors: Imai KS, Kobayashi K, Kari W, Rothbächer U, Ookubo N, Oda-Ishii I, Satou Y Tags: Dev Biol Source Type: research

Boundary maintenance in the ancestral metazoan Hydra depends on histone acetylation.
h TCG Abstract Much of boundary formation during development remains to be understood, despite being a defining feature of many animal taxa. Axial patterning of Hydra, a member of the ancient phylum Cnidaria which diverged prior to the bilaterian radiation, involves a steady-state of production and loss of tissue, and is dependent on an organizer located in the upper part of the head. We show that the sharp boundary separating tissue in the body column from head and foot tissue depends on histone acetylation. Histone deacetylation disrupts the boundary by affecting numerous developmental genes including Wnt compon...
Source: Developmental Biology - November 15, 2019 Category: Biology Authors: López-Quintero JA, Torres GG, Neme R, Bosch TCG Tags: Dev Biol Source Type: research

Building a worm: Complete development one cell at a time.
PMID: 31703772 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - November 12, 2019 Category: Biology Authors: Chalfie M Tags: Dev Biol Source Type: research

The zebrafish as a model for developmental and biomedical research in Poland and beyond.
PMID: 31705847 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - November 6, 2019 Category: Biology Authors: Winata CL, Dodzian J, Bialek-Wyrzykowska U Tags: Dev Biol Source Type: research

History of Zebrafish research in Poland.
PMID: 31705848 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - November 6, 2019 Category: Biology Authors: Tylzanowski P Tags: Dev Biol Source Type: research

Hhex regulates the specification and growth of the hepatopancreatic ductal system.
Abstract Significant efforts have advanced our understanding of foregut-derived organ development; however, little is known about the molecular mechanisms that underlie the formation of the hepatopancreatic ductal (HPD) system. Here, we report a role for the homeodomain transcription factor Hhex in directing HPD progenitor specification in zebrafish. Loss of Hhex function results in impaired HPD system formation. We found that Hhex specifies a distinct population of HPD progenitors that gives rise to the cystic duct, common bile duct, and extra-pancreatic duct. Since hhex is not uniquely expressed in the HPD regio...
Source: Developmental Biology - November 4, 2019 Category: Biology Authors: Villasenor A, Gauvrit S, Collins MM, Maischein HM, Stainier DYR Tags: Dev Biol Source Type: research

Development of the chick wing and leg neuromuscular systems and their plasticity in response to changes in digit numbers.
Abstract The tetrapod limb has long served as a paradigm to study vertebrate pattern formation. During limb morphogenesis, a number of distinct tissue types are patterned and subsequently must be integrated to form coherent functional units. For example, the musculoskeletal apparatus of the limb requires the coordinated development of the skeletal elements, connective tissues, muscles and nerves. Here, using light-sheet microscopy and 3D-reconstructions, we concomitantly follow the developmental emergence of nerve and muscle patterns in chicken wings and legs, two appendages with highly specialized locomotor outpu...
Source: Developmental Biology - November 4, 2019 Category: Biology Authors: Luxey M, Berki B, Heusermann W, Fischer S, Tschopp P Tags: Dev Biol Source Type: research

Epithelial-adipocyte interactions are required for mammary gland development, but not for milk production or fertility.
Abstract To investigate the role of adipose tissue in reproductive function and mammary gland development and function, we have examined lipodystrophic (LD) mice. LD mice of both sexes are sterile, but fertility can be restored with leptin injections. Mammary glands from lipodystrophic mice were rudimentary and lacked terminal end buds. Leptin-injected LD mice were able to become pregnant, showed normal pregnancy-associated glandular proliferation despite a smaller glandular area, were able to produce a small amount of milk that had grossly normal content of milk proteins and neutral lipids, but could not sustain ...
Source: Developmental Biology - November 4, 2019 Category: Biology Authors: Brenot A, Hutson I, Harris C Tags: Dev Biol Source Type: research

Huntingtin confers fitness but is not embryonically essential in zebrafish development.
Abstract Attempts to constitutively knockout HTT in rodents resulted in embryonic lethality, curtailing efforts to study HTT function later in development. Here we show that HTT is dispensable for early zebrafish development, contrasting published zebrafish morpholino experiment results. Homozygous HTT knockouts were embryonically viable and appeared developmentally normal through juvenile stages. Comparison of adult fish revealed significant reduction in body size and fitness in knockouts compared to hemizygotes and wildtype fish, indicating an important role for wildtype HTT in postnatal development. Our zebrafi...
Source: Developmental Biology - November 1, 2019 Category: Biology Authors: Sidik H, Ang CJ, Pouladi MA Tags: Dev Biol Source Type: research

Epigenetic transgenerational inheritance of parent-of-origin allelic transmission of outcross pathology and sperm epimutations.
Abstract Epigenetic transgenerational inheritance potentially impacts disease etiology, phenotypic variation, and evolution. An increasing number of environmental factors from nutrition to toxicants have been shown to promote the epigenetic transgenerational inheritance of disease. Previous observations have demonstrated that the agricultural fungicide vinclozolin and pesticide DDT (dichlorodiphenyltrichloroethane) induce transgenerational sperm epimutations involving DNA methylation, ncRNA, and histone modifications or retention. These two environmental toxicants were used to investigate the impacts of parent-of-...
Source: Developmental Biology - November 1, 2019 Category: Biology Authors: Ben Maamar M, King SE, Nilsson E, Beck D, Skinner MK Tags: Dev Biol Source Type: research

Hox13 is essential for formation of a sensory organ at the terminal end of the sperm duct in Ciona.
Abstract Species-specific traits are thought to have been acquired by natural selection. Transcription factors play central roles in the evolution of species-specific traits. Hox genes encode a set of conserved transcription factors essential for establishing the anterior-posterior body axis of animals. Changes in the expression or function of Hox genes can lead to the diversification of animal-body plans. The tunicate ascidian Ciona intestinalis Type A has an orange-colored structure at the sperm duct terminus. This orange-pigmented organ (OPO) is the characteristic that can distinguish this ascidian from other c...
Source: Developmental Biology - November 1, 2019 Category: Biology Authors: Tajima Y, Hozumi A, Yoshida K, Treen N, Sakuma T, Yamamoto T, Sasakura Y Tags: Dev Biol Source Type: research

From bench to board-side: Academic teaching careers.
Abstract Teaching positions provide a rewarding career pathway for Ph.D.s wishing to stay in academia outside of the research-focused position. The balance of teaching and research expectations on faculty can vary greatly depending on the type of institution. Faculty at primarily undergraduate institutions may be required to be research active and mentor undergraduates in the laboratory, while teaching faculty at a research-centered university may not have a research lab. In addition, faculty are expected to actively contribute to the shared governance of the institution, in the name of service. The career in teac...
Source: Developmental Biology - November 1, 2019 Category: Biology Authors: Freeman EA, Theodosiou NA, Anderson WJ Tags: Dev Biol Source Type: research

Best of both worlds: A career in technology transfer and business development.
Abstract The current academic and business landscape does not provide a clear path for doctoral trainees graduating. Moreover, the fundamental need of the hour is diversification of the workforce where PhD graduates need to be trained to do more beyond basic scientific research. One of the possible paths that is now becoming popular as science enters its translational phase is research commercialization and technology transfer. This short article provides an comprehensive overview of the technology transfer field and possible paths trainees can take to position themselves as a successful technology transfer profes...
Source: Developmental Biology - November 1, 2019 Category: Biology Authors: Sachani SS Tags: Dev Biol Source Type: research

A systems-level view of pattern formation mechanisms in development.
r P PMID: 31678369 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 31, 2019 Category: Biology Authors: El-Sherif E, Müller P Tags: Dev Biol Source Type: research

Life beyond the bench: Scientific writing.
PMID: 31669343 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 26, 2019 Category: Biology Authors: Hatch V Tags: Dev Biol Source Type: research

From the bench to the press: Scholarly publishing as a career route for the scientist.
Abstract As the academic career path has become less feasible for most researchers, scholarly publishing offers a way to put scientific training to use, and to build a career while remaining in the world of research. Here I detail the path that my career took and offer advice for those interested in exploring academic publishing. PMID: 31669334 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 25, 2019 Category: Biology Authors: Crotty D Tags: Dev Biol Source Type: research

FRS2 α-dependent cell fate transition during endocardial cushion morphogenesis.
FRS2α-dependent cell fate transition during endocardial cushion morphogenesis. Dev Biol. 2019 Oct 25;: Authors: Chen D, Zhu X, Kofler N, Wang Y, Zhou B, Simons M Abstract Atrioventricular valve development requires endothelial-to-mesenchymal transition (EndMT) that induces cushion endocardial cells to give rise to mesenchymal cells crucial to valve formation. In the adult endothelium, deletion of the docking protein FRS2α induces EndMT by activating TGFβ signaling in a miRNA let-7-dependent manner. To study the role of endothelial FRS2α during embryonic development, we generated...
Source: Developmental Biology - October 25, 2019 Category: Biology Authors: Chen D, Zhu X, Kofler N, Wang Y, Zhou B, Simons M Tags: Dev Biol Source Type: research

Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate.
Abstract Lens abnormalities are a major cause of reduced vision and blindness. One mechanism that can lead to reduced lens transparency, i.e. cataract, is abnormal behavior of lens epithelial cells (LECs), the precursors of the transparent lens fiber cells. Here we describe a zebrafish mutation causing the embryonic lens epithelium to generate cellular masses comprising partially differentiated lens fiber cells. We identify the mutant gene as plod3, which encodes for Lysyl hydroxylase 3 (Lh3), an enzyme essential for modification of collagens, including Collagen IV, a main component of the lens capsule. We show th...
Source: Developmental Biology - October 24, 2019 Category: Biology Authors: Taler K, Weiss O, Rotem S, Rubinstein AM, Seritrakul P, Gross JM, Inbal A Tags: Dev Biol Source Type: research

Beyond the lab bench: Pathways in inclusion, equity, and diversity in biology education and social justice.
Abstract Over the past six years, I describe my personal journey into promoting inclusion, equity, and diversity in biology education and social justice. In my personal journey, I will describe how I found my passion in mentoring and teaching Native American, Latinx, and non-traditional undergraduates in cell and developmental biology. I will also describe how Dr. Karen Gross' concept of lasticity aligned with culturally-responsive teaching and mentoring can help foster transformative learning for all undergraduates. PMID: 31647908 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 21, 2019 Category: Biology Authors: Kao RM Tags: Dev Biol Source Type: research

A Mentor's perspective: It's not all about academic research-other careers for Ph. D.s in developmental biology and biological sciences.
PMID: 31639335 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 19, 2019 Category: Biology Authors: Erickson RP Tags: Dev Biol Source Type: research

The road taken -changing One's professional focus at a large research university.
Abstract The scientific endeavor has many facets, extending well beyond the experimental research bench. However, in most fields, especially in the biomedical sciences, the traditional career pathway for scientists is first joining and later leading an experimental research laboratory or program. As a result, scientific education is often focused on training new bench researchers. My own journey from a traditional bench scientist to that of an educator and educational researcher will be discussed in the context of a large research university environment. Being a scientist with an educational focus at such an insti...
Source: Developmental Biology - October 19, 2019 Category: Biology Authors: Hortsch M Tags: Dev Biol Source Type: research

Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.
lfi A Abstract The central nervous system of the Ciona larva contains only 177 neurons. The precise regulation of neuron subtype-specific morphogenesis and differentiation observed during the formation of this minimal connectome offers a unique opportunity to dissect gene regulatory networks underlying chordate neurodevelopment. Here we compare the transcriptomes of two very distinct neuron types in the hindbrain/spinal cord homolog of Ciona, the Motor Ganglion (MG): the Descending decussating neuron (ddN, proposed homolog of Mauthner Cells in vertebrates) and the MG Interneuron 2 (MGIN2). Both types are invariant...
Source: Developmental Biology - October 19, 2019 Category: Biology Authors: Gibboney S, Orvis J, Kim K, Johnson CJ, Martínez-Feduchi P, Lowe EK, Sharma S, Stolfi A Tags: Dev Biol Source Type: research

Cytoglobin deficiency potentiates Crb1-mediated retinal degeneration in rd8 mice.
CONCLUSIONS: Cygb is necessary for retinal lamination, maintenance of cell polarity, and photoreceptor survival in rd8 mice. These results are consistent with Cygb as a disease modifying gene in Crb1-related retinopathy. Further studies are necessary to investigate the role of Cygb in the human retina. PMID: 31634437 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 18, 2019 Category: Biology Authors: Kwon YS, Tham A, Lopez AJ, Edwards S, Woods S, Chen J, Fortunato J, Quiroz A, Javier S, Au I, Clarke M, Humpal D, Lloyd KCK, Thomasy S, Murphy C, Glaser TM, Moshiri A Tags: Dev Biol Source Type: research

20/20 Hindsight - A chance to reflect upon my career.
PMID: 31634438 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - October 18, 2019 Category: Biology Authors: Frank M Tags: Dev Biol Source Type: research

The enteric nervous system undergoes significant chemical and synaptic maturation during adolescence in mice.
Abstract Adolescence is a critical period of development. It is very likely that there is significant maturation of the enteric nervous system (ENS) of the gut during this stage of life, especially since there are substantial changes in factors known to influence the ENS including diet and microbiota during this time, but this remains unknown. To examine maturation of the ENS during adolescence, we performed immunohistochemistry using advanced microscopy and analytical methods to compare enteric neurons and glia of the duodenum and colon of mice taken prior to weaning with those of young adult mice. We found signi...
Source: Developmental Biology - October 17, 2019 Category: Biology Authors: Parathan P, Wang Y, Leembruggen AJ, Bornstein JC, Foong JP Tags: Dev Biol Source Type: research

Amphiregulin mediates the hormonal regulation on Rspondin-1 expression in the mammary gland.
Abstract The steroid hormones are instrumental for the growth of mammary epithelial cells. Our previous study indicates that hormones regulate the expression of Rspondin-1 (Rspo1). Yet, the regulatory mechanism remains unknown. In the current study, we identify Amphiregulin (Areg) as a novel upstream regulator of Rspo1 expression mediating the hormonal influence. In response to hormonal signaling, Areg emanating from estrogen receptor (ER)-positive luminal cells, induce the expression of Rspo1 in ER-negative luminal cells. The paracrine action of Areg on Rspo1 expression is dependent on Egfr. Our data reveal a nov...
Source: Developmental Biology - October 11, 2019 Category: Biology Authors: Cai C, Geng A, Wang M, Yang L, Yu QC, Zeng YA Tags: Dev Biol Source Type: research

Blastula stage specification of avian neural crest.
ro MI Abstract Cell fate specification defines the earliest steps towards a distinct cell lineage. Neural crest, a multipotent stem cell population, is thought to be specified from the ectoderm, but its varied contributions defy canons of segregation potential and challenges its embryonic origin. Aiming to resolve this conflict, we have assayed the earliest specification of neural crest using blastula stage chick embryos. Specification assays on isolated chick epiblast explants identify an intermediate region specified towards the neural crest cell fate. Furthermore, low density culture suggests that the specifica...
Source: Developmental Biology - October 11, 2019 Category: Biology Authors: Prasad MS, Uribe-Querol E, Marquez J, Vadasz S, Yardley N, Shelar PB, Charney RM, García-Castro MI Tags: Dev Biol Source Type: research

A developmental perspective on the evolution of the nervous system.
M, Hejnol A Abstract The evolution of nervous systems in animals has always fascinated biologists, and thus multiple evolutionary scenarios have been proposed to explain the appearance of neurons and complex neuronal centers. However, the absence of a robust phylogenetic framework for animal interrelationships, the lack of a mechanistic understanding of development, and a recapitulative view of animal ontogeny have traditionally limited these scenarios. Only recently, the integration of advanced molecular and morphological studies in a broad range of animals has allowed tracing the evolution of developmental and n...
Source: Developmental Biology - October 11, 2019 Category: Biology Authors: Martín-Durán JM, Hejnol A Tags: Dev Biol Source Type: research

Slit neuronal secretion coordinates optic lobe morphogenesis in Drosophila.
rralta J, Oliva C Abstract The complexity of the nervous system requires the coordination of multiple cellular processes during development. Among them, we find boundary formation, axon guidance, cell migration and cell segregation. Understanding how different cell populations such as glial cells, developing neurons and neural stem cells contribute to the formation of boundaries and morphogenesis in the nervous system is a critical question in neurobiology. Slit is an evolutionary conserved protein essential for the development of the nervous system. For signaling, Slit has to bind to its cognate receptor Robo, a ...
Source: Developmental Biology - October 10, 2019 Category: Biology Authors: Caipo L, González-Ramírez MC, Guzmán-Palma P, Contreras EG, Palominos T, Fuenzalida-Uribe N, Hassan BA, Campusano JM, Sierralta J, Oliva C Tags: Dev Biol Source Type: research

Insights into intestinal regeneration signaling mechanisms.
V, Amador A, Reyes J, García-Arrarás JE Abstract The cellular mechanisms underlying the amazing ability of sea cucumbers to regenerate their autotomized intestines have been widely described by us and others. However, the signaling pathways that control these mechanisms are unknown. Previous studies have shown that Wnt homologs are upregulated during early intestinal regenerative stages, suggesting that the Wnt/β-catenin pathway is active during this process. Here, we used small molecules, putative disruptors of the Wnt pathway, to determine the potential role of the canonical Wnt pathway on int...
Source: Developmental Biology - October 9, 2019 Category: Biology Authors: Bello SA, Torres-Gutiérrez V, Rodríguez-Flores EJ, Toledo-Román EJ, Rodríguez N, Díaz-Díaz LM, Vázquez-Figueroa LD, Cuesta JM, Grillo-Alvarado V, Amador A, Reyes J, García-Arrarás JE Tags: Dev Biol Source Type: research

LongAxis: A MATLAB-based program for 3D quantitative analysis of epithelial cell shape and orientation.
Abstract Epithelial morphogenesis, a fundamental aspect of development, generates 3-dimensional tissue structures crucial for organ function. Underlying morphogenetic mechanisms are, in many cases, poorly understood, but mutations that perturb organ development can affect epithelial cell shape and orientation - difficult features to quantify in three dimensions. The basic structure of the eye is established via epithelial morphogenesis: in the embryonic optic cup, the retinal progenitor epithelium enwraps the lens. We previously found that loss of the extracellular matrix protein laminin-alpha1 (lama1) led to misl...
Source: Developmental Biology - October 4, 2019 Category: Biology Authors: Carney KR, Bryan CD, Gordon HB, Kwan KM Tags: Dev Biol Source Type: research

Alveolar progenitor cells in the mammary gland are dependent on the β4 integrin.
Alveolar progenitor cells in the mammary gland are dependent on the β4 integrin. Dev Biol. 2019 Oct 03;: Authors: Walker MR, Amante JJ, Li J, Liu H, Zhu LJ, Feltri ML, Goel HL, Mercurio AM Abstract Understanding how progenitor cell function is regulated in the mammary gland is an important developmental problem that has significant implications for breast cancer. Although it had been assumed that the expression the α6β4 integrin (β4) is restricted to the basal lineage, we report that alveolar progenitor cells in the mouse mammary gland also express this integrin based on analysis ...
Source: Developmental Biology - October 3, 2019 Category: Biology Authors: Walker MR, Amante JJ, Li J, Liu H, Zhu LJ, Feltri ML, Goel HL, Mercurio AM Tags: Dev Biol Source Type: research

Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation.
Abstract Yap/Taz are well-established downstream effectors of the Hippo pathway, known to regulate organ size by directing proliferation and apoptosis. Although the functions of Yap/Taz have been extensively studied, little is known about their role in brain development. Here, through genetic ablation, we show that Yap/Taz are required for cerebellar morphogenesis. Yap/Taz deletion in neural progenitors causes defects in secondary fissure formation, leading to abnormal folia development. Although they seemed very likely to serve an important function in the development of cerebellar granule cell precursors, Yap/Ta...
Source: Developmental Biology - October 3, 2019 Category: Biology Authors: Hughes LJ, Park R, Lee MJ, Terry BK, Lee DJ, Kim H, Cho SH, Kim S Tags: Dev Biol Source Type: research

Commentary on paper by Leroy C.
PMID: 31470964 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - September 1, 2019 Category: Biology Authors: Duboule D Tags: Dev Biol Source Type: research

Commentary on Fristrom (1976).
PMID: 31358283 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - August 1, 2019 Category: Biology Authors: Keller R Tags: Dev Biol Source Type: research

Commentary on classic paper "Gimlich, R.L., Gerhart, J.C., 1984. Early cellular interactions promote embryonic axis formation in Xenopus laevis. Dev Biol 104, 117-130."
Commentary on classic paper "Gimlich, R.L., Gerhart, J.C., 1984. Early cellular interactions promote embryonic axis formation in Xenopus laevis. Dev Biol 104, 117-130." Dev Biol. 2019 Aug 15;452(2):67-82 Authors: Harland R PMID: 31235010 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - June 28, 2019 Category: Biology Authors: Harland R Tags: Dev Biol Source Type: research

Commentary and tribute to Antone Jacobson: The pioneer of morphodynamics.
PMID: 31229196 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - June 25, 2019 Category: Biology Authors: Wallingford JB, Sater AK Tags: Dev Biol Source Type: research

Pericardin, a Drosophila collagen, facilitates accumulation of hemocytes at the heart.
Abstract Hematopoietic cell lineages support organismal needs by responding to positional and systemic signals that balance proliferative and differentiation events. Drosophila provides an excellent genetic model to dissect these signals, where the activity of cues in the hemolymph or substrate can be traced to determination and differentiation events of well characterized hemocyte types. Plasmatocytes in third instar larvae increase in number in response to infection and in anticipation of metamorphosis. Here we characterize hemocyte clustering, proliferation and transdifferentiation on the heart or dorsal vessel...
Source: Developmental Biology - June 19, 2019 Category: Biology Authors: Cevik D, Acker M, Michalski C, Jacobs JR Tags: Dev Biol Source Type: research

Fibroblast growth factor signaling mediates progenitor cell aggregation and nephron regeneration in the adult zebrafish kidney.
Abstract The zebrafish kidney regenerates after injury by development of new nephrons from resident adult kidney stem cells. Although adult kidney progenitor cells have been characterized by transplantation and single cell RNA seq, signals that stimulate new nephron formation are not known. Here we demonstrate that fibroblast growth factors and FGF signaling is rapidly induced after kidney injury and that FGF signaling is required for recruitment of progenitor cells to sites of new nephron formation. Chemical or dominant negative blockade of Fgfr1 prevented formation of nephron progenitor cell aggregates after inj...
Source: Developmental Biology - June 17, 2019 Category: Biology Authors: Gallegos TF, Kamei CN, Rohly M, Drummond IA Tags: Dev Biol Source Type: research

Evolution of Snail-mediated regulation of neural crest and placodes from an ancient role in bilaterian neurogenesis.
Abstract A major challenge in vertebrate evolution is to identify the gene regulatory mechanisms that facilitated the origin of neural crest cells and placodes from ancestral precursors in invertebrates. Here, we show in lamprey, a primitively jawless vertebrate, that the transcription factor Snail is expressed simultaneously throughout the neural plate, neural plate border, and pre-placodal ectoderm in the early embryo and is then upregulated in the CNS throughout neurogenesis. Using CRISPR/Cas9-mediated genome editing, we demonstrate that Snail plays functional roles in all of these embryonic domains or their de...
Source: Developmental Biology - June 15, 2019 Category: Biology Authors: York JR, Zehnder K, Yuan T, Lakiza O, McCauley DW Tags: Dev Biol Source Type: research

Commentary on "Direct visualization, by β-galactosidase histochemistry, of differentiated normal cells derived from malignant teratocarcinoma in allophenic mice" by Dewey and Mintz 1978.
Commentary on "Direct visualization, by β-galactosidase histochemistry, of differentiated normal cells derived from malignant teratocarcinoma in allophenic mice" by Dewey and Mintz 1978. Dev Biol. 2019 Jun 15;450(2):65-75 Authors: Capel B PMID: 31138433 [PubMed - in process] (Source: Developmental Biology)
Source: Developmental Biology - May 30, 2019 Category: Biology Authors: Capel B Tags: Dev Biol Source Type: research

Lens differentiation is characterized by stage-specific changes in chromatin accessibility correlating with differentiation state-specific gene expression.
Abstract Changes in chromatin accessibility regulate the expression of multiple genes by controlling transcription factor access to key gene regulatory sequences. Here, we sought to establish a potential function for altered chromatin accessibility in control of key gene expression events during lens cell differentiation by establishing genome-wide chromatin accessibility maps specific for four distinct stages of lens cell differentiation and correlating specific changes in chromatin accessibility with genome-wide changes in gene expression. ATAC sequencing was employed to generate chromatin accessibility profiles...
Source: Developmental Biology - May 25, 2019 Category: Biology Authors: Disatham J, Chauss D, Gheyas R, Brennan L, Blanco D, Daley L, Menko AS, Kantorow M Tags: Dev Biol Source Type: research

The developing ocular lens: A practical paradigm for probing chromatin landscape and gene expression.
PMID: 31129080 [PubMed - as supplied by publisher] (Source: Developmental Biology)
Source: Developmental Biology - May 23, 2019 Category: Biology Authors: Bhat SP Tags: Dev Biol Source Type: research

Lineage tracing of sclerotome cells in amphibian reveals that somitic multipotent cells originate from lateral somitic frontier.
ine C Abstract The two somite compartments, dorso-lateral dermomyotome and medio-ventral sclerotome are major vertebrate novelties, but little is known about their evolutionary origin. We determined that sclerotome cells in Xenopus come from lateral somitic frontier (LSF) by lineage tracing, ablation experiments and histological analysis. We identified Twist1 as marker of migrating sclerotome progenitors in two amphibians, Xenopus and axolotl. From these results, three conclusions can be drawn. First, LSF is made up of multipotent somitic cells (MSCs) since LSF gives rise to sclerotome but also to dermomytome as a...
Source: Developmental Biology - May 22, 2019 Category: Biology Authors: Della Gaspera B, Mateus A, Andéol Y, Weill L, Charbonnier F, Chanoine C Tags: Dev Biol Source Type: research

PTK7 proteolytic fragment proteins function during early Xenopus development.
In this study we examined the biological activity of two proteolytic carboxyl-terminal PTK7 proteolytic fragments, cPTK7 622-1070 and cPTK7 726-1070 during early Xenopus nervous system development. We found that these smaller PTK7 proteolytic fragments have similar activity to full-length PTK7 protein to promote canonical Wnt-signaling via regulation of LRP6 protein levels. In addition to cancer systems, this study shows in vivo proof that these smaller PTK7 proteolytic fragments can recapitulate full-length PTK7 protein activity in diverse systems, such as vertebrate nervous system development. PMID: 31125531 [PubMed...
Source: Developmental Biology - May 21, 2019 Category: Biology Authors: Lichtig H, Cohen Y, Bin-Nun N, Golubkov V, Frank D Tags: Dev Biol Source Type: research

Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development.
Abstract The origin of the mammalian lymphatic vasculature has been studied for more than a century; however, details regarding organ-specific lymphatic development remain unknown. A recent study reported that cardiac lymphatic endothelial cells (LECs) stem from venous and non-venous origins in mice. Here, we identified Isl1-expressing progenitors as a potential non-venous origin of cardiac LECs. Genetic lineage tracing with Isl1-Cre reporter mice suggested a possible contribution from the Isl1-expressing pharyngeal mesoderm constituting the second heart field to lymphatic vessels around the cardiac outflow tract ...
Source: Developmental Biology - May 18, 2019 Category: Biology Authors: Maruyama K, Miyagawa-Tomita S, Mizukami K, Matsuzaki F, Kurihara H Tags: Dev Biol Source Type: research