siRNA transfection in the barnacle Amphibalanus amphitrite larvae [METHODS [amp ] TECHNIQUES]

In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either nauplius or cyprid stage, or both. Effects of siRNA transfection on p38 MAPK level were hardly detectable in the cyprids that their corresponding nauplii were transfected. In contrast, larvae that were transfected at cyprid stage showed lower level of p38 MAPK than the blank and reagent controls. However, significantly decreased level of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rate were observed only in the "Double Transfection", in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. Relatively longer transfection time and more cells of the larvae exposed to siRNA directly might explain the higher efficiency in the "Double Transfection".

Source: Journal of Experimental Biology - June 25, 2015 Category: Biology Authors: Zhang, G., He, L.-s., Wong, Y. H., Yu, L., Qian, P.-y. Tags: METHODS [amp ] TECHNIQUES Source Type: research