Exploring the resistome, virulome, and mobilome of multidrug-resistant Klebsiella pneumoniae isolates: deciphering the molecular basis of carbapenem resistance
Klebsiella pneumoniae, a notorious pathogen for causing nosocomial infections has become a major cause of neonatal septicemia, leading to high morbidity and mortality worldwide. This opportunistic bacterium has ... (Source: BMC Genomics)
Source: BMC Genomics - April 25, 2024 Category: Genetics & Stem Cells Authors: Sidra Rahmat Ullah, Sidra Irum, Iqra Mahnoor, Humaira Ismatullah, Mariam Mumtaz, Saadia Andleeb, Abdur Rahman and Muhsin Jamal Tags: Research Source Type: research
A PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application
Sequencing the mitochondrial genome has been increasingly important for the investigation of primary mitochondrial diseases (PMD) and mitochondrial genetics. To overcome the limitations originating from PCR-ba... (Source: Journal of Translational Medicine)
Source: Journal of Translational Medicine - April 25, 2024 Category: Research Authors: Dong Liang, Lin Zhu, Yuqing Zhu, Mingtao Huang, Ying Lin, Hang Li, Ping Hu, Jun Zhang, Bin Shen and Zhengfeng Xu Tags: Methodology Source Type: research
GMOIT: a tool for effective screening of genetically modified crops
Advancement in agricultural biotechnology has resulted in increasing numbers of commercial varieties of genetically modified (GM) crops worldwide. Though several databases on GM crops are available, these data... (Source: BMC Plant Biology)
Source: BMC Plant Biology - April 25, 2024 Category: Biology Authors: Pu Zhou, Xuan Liu, Jingang Liang, Juanli Zhao, Yuqi Zhang, Dongmei Xu, Xiaying Li, Ziyan Chen, Zongyong Shi and Jianhua Gao Tags: Research Source Type: research
Assessing several fungal contaminants and their associated mycotoxins in maize cultivated on cornfields of Republic of Moldova
. (Source: Food Additives and Contaminants Part A)
Source: Food Additives and Contaminants Part A - April 25, 2024 Category: Food Science Authors: Cristina GrajdieruIrina MitinaLidia TumanovaValentin MitinLaboratory of Molecular genetics, Institute of Genetics, Physiology and Plant Protection of Moldova State University, Chisinau, Republic of Moldova Source Type: research
Enhanced cellular therapy: revolutionizing adoptive cellular therapy
This article comprehensively summarizes the mechanism, current situation, and clinical application of enhanced cellular therapy, describing potential improvements to adoptive cellular therapy. (Source: Experimental Hematology and Oncology)
Source: Experimental Hematology and Oncology - April 25, 2024 Category: Cancer & Oncology Source Type: research
Genetic parameters, genetic trends and correlated responses of growth traits considering maternal ability in Nelore cattle
AbstractThe growth of Nelore cattle was analysed considering the following performance parameters; the effect of the calving order of cows on the phenotypic expression of birth weight (BW), average daily gain from birth to weaning (BWG), and weaning weight (WW), the estimated genetic parameters for the traits, including the covariance components between direct and maternal genetic effects. Genetic trends and correlated responses were also obtained for the studied traits. The calving order of cows, as well as other fixed effects used to obtain the adjusted phenotypic means, were statistically significant (p < 0.001)...
Source: Tropical Animal Health and Production - April 25, 2024 Category: Veterinary Research Source Type: research
HPV infection and its correlation with p53 and Bcl-2 among pregnant mothers and their infants
AbstractThe investigation of perinatal transmission of HPV is vital for early screening of cervical/oral cancers. Here, transmission of HPV from the pregnant women to their infants was studied. p53 and Bcl-2 expressions and their correlations with HPV infection were examined. HPV infection was detected in the cervical and oral swabs of 135 mother-baby pairs employing both PCR and HC-II methods. 1 year follow-up with an interim visit at 3 months for mothers and 6 months for babies was performed. Immunocytochemistry of p53 and Bcl-2 using the streptavidin–biotin peroxidase method was performed. Prevalence of HPV infect...
Source: Virus Genes - April 25, 2024 Category: Genetics & Stem Cells Source Type: research
Danish Diabetes Birth Registry 2: a study protocol of a national prospective cohort study to monitor outcomes of pregnancies of women with pre-existing diabetes
Introduction
Despite technological developments and intensified care, pregnancies in women with pre-existing diabetes are still considered high-risk pregnancies. The rate of adverse outcomes in pregnancies affected by diabetes in Denmark is currently unknown, and there is a limited understanding of mechanisms contributing to this elevated risk. To address these gaps, the Danish Diabetes Birth Registry 2 (DDBR2) was established. The aims of this registry are to evaluate maternal and fetal-neonatal outcomes based on 5 years cohort data, and to identify pathophysiology and risk factors associated with short-term and long-term...
Source: BMJ Open - April 25, 2024 Category: General Medicine Authors: Knorr, S., Aalders, J., Overgaard, M., Stovring, H., Mathiesen, E. R., Damm, P., Clausen, T. D., Bjerre-Christensen, U., Andersen, L. L. T., Vinter, C., Kofoed-Enevoldsen, A., Lauenborg, J., Kampmann, U., Fuglsang, J., Ovesen, P. G., Christensen, T. T., S Tags: Open access, Diabetes and Endocrinology Source Type: research
Endometriosis, a common but enigmatic disease with many faces: current concept of pathophysiology, and diagnostic strategy
AbstractEndometriosis is a benign, common, but controversial disease due to its enigmatic etiopathogenesis and biological behavior. Recent studies suggest multiple genetic, and environmental factors may affect its onset and development. Genomic analysis revealed the presence of cancer-associated gene mutations, which may reflect the neoplastic aspect of endometriosis. The management has changed dramatically with the development of fertility-preserving, minimally invasive therapies. Diagnostic strategies based on these recent basic and clinical findings are reviewed. With a focus on the presentation of clinical cases, we di...
Source: Japanese Journal of Radiology - April 25, 2024 Category: Radiology Source Type: research
From the Microscopic Sweat Gland to Large Datasets: The Power of Quantitative Traits in Dermatology
Sweat gland density (SGD) is a cutaneous trait that varies naturally within and across populations and also contributes to disease pathologies. In their recent article in Journal of Investigative Dermatology, Chen et al. (2024) report a GWAS of SGD that illustrates how genetic studies of quantitative skin traits provide an efficient method for determining the molecular basis of dermatologic diseases. This work also provides an opportunity to highlight the many modalities available to collect quantitative data about skin features that have relevance to cutaneous diseases. (Source: Journal of Investigative Dermatology)
Source: Journal of Investigative Dermatology - April 25, 2024 Category: Dermatology Authors: Olivia D. Perez, Eric R. Tkaczyk, Lynn Petukhova Tags: Commentary Source Type: research
The kinase Rio1 and a ribosome collision-dependent decay pathway survey the integrity of 18S rRNA cleavage
by Melissa D. Parker, Elise S. Brunk, Adam J. Getzler, Katrin Karbstein
The 18S rRNA sequence is highly conserved, particularly at its 3 ′-end, which is formed by the endonuclease Nob1. How Nob1 identifies its target sequence is not known, and in vitro experiments have shown Nob1 to be error-prone. Moreover, the sequence around the 3′-end is degenerate with similar sites nearby. Here, we used yeast genetics, biochemistry, and nex t-generation sequencing to investigate a role for the ATPase Rio1 in monitoring the accuracy of the 18S rRNA 3′-end. We demonstrate that Nob1 can miscleave its rRNA substrate and that miscl...
Source: PLoS Biology: Archived Table of Contents - April 25, 2024 Category: Biology Authors: Melissa D. Parker Source Type: research
Non-Invasive Preimplantation Genetic Testing for Aneuploidy – Look Before You Leap
(Source: Fertility and Sterility)
Source: Fertility and Sterility - April 25, 2024 Category: Reproduction Medicine Authors: Francesca Barrett, Thomas Molinaro Tags: Reflections Source Type: research
The genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion
by Alba Pacheco-Moreno, Anita Bollmann-Giolai, Govind Chandra, Paul Brett, Jack Davies, Owen Thornton, Philip Poole, Vinoy Ramachandran, James K. M. Brown, Paul Nicholson, Chris Ridout, Sarah DeVos, Jacob G. Malone
Plant-associated microbes play vital roles in promoting plant growth and health, with plants secreting root exudates into the rhizosphere to attract beneficial microbes. Exudate composition defines the nature of microbial recruitment, with different plant species attracting distinct microbiota to enable optimal adaptation to the soil environment. To more closely examine the relationship between plant genotype a...
Source: PLoS Biology: Archived Table of Contents - April 25, 2024 Category: Biology Authors: Alba Pacheco-Moreno Source Type: research
Ecological drift during colonization drives within-host and between-host heterogeneity in an animal-associated symbiont
by Jason Z. Chen, Zeeyong Kwong, Nicole M. Gerardo, Nic M. Vega
Specialized host –microbe symbioses canonically show greater diversity than expected from simple models, both at the population level and within individual hosts. To understand how this heterogeneity arises, we utilize the squash bug,Anasa tristis, and its bacterial symbionts in the genusCaballeronia. We modulate symbiont bottleneck size and inoculum composition during colonization to demonstrate the significance of ecological drift, the noisy fluctuations in community composition due to demographic stochasticity. Consistent with predictions from the neutra...
Source: PLoS Biology: Archived Table of Contents - April 25, 2024 Category: Biology Authors: Jason Z. Chen Source Type: research
Aminotransferases as causal factors for metabolic syndrome: A bidirectional Mendelian randomization study
ConclusionsOur bidirectional MR analyses suggest that ALT and AST are causally associated with several metabolic syndrome-related traits, especially hypertension and type 2 diabetes. These findings highlight the potential role of aminotransferases as biomarkers and therapeutic targets for metabolic syndrome. (Source: PLoS One)
Source: PLoS One - April 25, 2024 Category: Biomedical Science Authors: Meng Lingyu Source Type: research
