Monoacylglycerol lipase blockade impairs fine motor coordination and triggers cerebellar neuroinflammation through cyclooxygenase-2
Publication date: Available online 25 June 2019Source: Brain, Behavior, and ImmunityAuthor(s): Sara Martínez-Torres, Laura Cutando, Antoni Pastor, Ako Kato, Kenji Sakimura, Rafael de la Torre, Emmanuel Valjent, Rafael Maldonado, Masanobu Kano, Andrés OzaitaAbstractMonoacylglycerol lipase (MAGL) is the main enzyme implicated in the degradation of the most abundant endocannabinoid in the brain, 2-arachidonoylglycerol (2-AG), producing arachidonic acid (AA) and glycerol. MAGL pharmacological inhibition with JZL184 or genetic deletion results in an exacerbated 2-AG signaling and reduced synthesis of prostaglandin...
Source: Brain, Behavior, and Immunity - June 25, 2019 Category: Neurology Source Type: research

On sulfonamide resistance, sul genes, class 1 integrons and their horizontal transfer in Escherichia coli
Publication date: Available online 25 June 2019Source: Microbial PathogenesisAuthor(s): María Eloísa Poey, María F. Azpiroz, Magela LaviñaAbstractClass 1 integrons (Int1) contribute to antibiotic multiresistance in Gram-negative bacteria. Being frequently carried by conjugative plasmids, their spread would depend to some extent on their horizontal transfer to other bacteria. This was the main issue that was addressed in this work: the analysis of Int1 lateral transfer in the presence of different antibiotic pressures. Strains from a previously obtained collection of Escherichia coli K12 carrying...
Source: Microbial Pathogenesis - June 25, 2019 Category: Infectious Diseases Source Type: research

Investigating the role of LSD2 as an epigenetic regulator in Ewing sarcoma.
Authors: Patel PO, Pishas KI, Taslim C, Selich-Anderson J, Theisen ER, Lessnick SL Abstract Ewing sarcoma is the second most common solid bone malignancy diagnosed in pediatric and young adolescent populations. Despite aggressive multi-modal treatment strategies, 5-year event-free survival remains at 75% for patients with localized disease and 20% for patients with metastases. Thus, the need for novel therapeutic options is imperative. Recent studies have focused on epigenetic misregulation in Ewing sarcoma development and potential new oncotargets for treatment. This project focused on the study of LSD2, a flavin-...
Source: Oncotarget - June 25, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Functional characterization of CNOT3 variants identified in familial adenomatous polyposis adenomas.
Authors: Delacruz RGC, Sandoval IT, Chang K, Miller BN, Reyes-Uribe L, Borras E, Lynch PM, Taggart MW, Hawk ET, Vilar E, Jones DA Abstract Germline mutations in the tumor suppressor Adenomatous Polyposis Coli (APC) define Familial Adenomatous Polyposis (FAP), the genetic predisposition to developing adenomatous polyps. Recent sequencing of FAP adenomas have challenged established dogma that APC mutations alone represent the adenoma mutational landscape because recurrent somatic mutations in non-WNT pathway genes were also discovered. In particular, one of these novel genes, CNOT3, presented E20K and E70K mutations ...
Source: Oncotarget - June 25, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

An efficient shortened genetic transformation strategy for filamentous fungus Trichoderma reesei.
Authors: Wu C, Chen Y, Huang X, Sun S, Luo J, Lu Z, Wang W, Ma Y Abstract The filamentous fungus Trichoderma reesei is one of the most important fungi for the production of cellulases and xylanases, which can be used for biofuel production from lignocellulose. We aimed to develop an effective selection marker system for more extensive functional genomic studies in the fungus T. reesei, and to construct better industrial transformants for producing cellulases. Here, we present a novel effective G418 selection marker to use a codon-optimized neomycin phosphotransferase II gene nptII to transform T. reesei. We develop...
Source: Journal of General and Applied Microbiology - June 25, 2019 Category: Microbiology Tags: J Gen Appl Microbiol Source Type: research

Maternal BMI-Increasing Genetic Risk Score and Fetal Weights among Diverse US Ethnic Groups.
Abstract OBJECTIVE: Associations between maternal genetic risk for obesity and fetal weight were examined at the end of the first (13 weeks 6 days), second (27 weeks 6 days), and third (40 weeks 0 days) trimesters of pregnancy among four race/ethnic groups in the US. METHODS: For 603 white, 591 black, 535 Hispanic, and 216 Asian women, maternal genetic risk score (GRS) was calculated as the sum of 189 BMI-increasing alleles and was categorized into high or low GRS. Associations between GRS (continuous and categorical) and estimated fetal weight were tested overall and stratified by prepregnancy BMI, gestation...
Source: Obesity - June 25, 2019 Category: Eating Disorders & Weight Management Authors: Shrestha D, Rahman ML, Hinkle SN, Workalemahu T, Tekola-Ayele F Tags: Obesity (Silver Spring) Source Type: research

GSE133249 Identification of an eight-protein biosignature for the diagnosis of tuberculosis
Contributors : Ruo-Pan  Huang ; Xinchun ChenSeries Type : Protein profiling by protein arrayOrganism : Homo sapiensMycobacterium tuberculosis (Mtb) antigen-specific cellular response is promising for detectionof Mtb infection, but not efficient for diagnosis of TB. We firstly identified 16 TB disease-specific protein markers measured in the culture supernatant of Mtb-stimulated whole blood using a 640 human proteins array, the highest throughput antibody-based protein array available at the time when we did this study. Potential TB-related proteins were then analyzed across three different patient cohorts comprised of...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Protein profiling by protein array Homo sapiens Source Type: research

Cathepsin K–deficient osteocytes prevent lactation-induced bone loss and parathyroid hormone suppression
Lactation induces bone loss to provide sufficient calcium in the milk, a process that involves osteoclastic bone resorption but also osteocytes and perilacunar resorption. The exact mechanisms by which osteocytes contribute to bone loss remain elusive. Osteocytes express genes required in osteoclasts for bone resorption, including cathepsin K (Ctsk), and lactation elevates their expression. We show that Ctsk deletion in osteocytes prevented the increase in osteocyte lacunar area seen during lactation, as well as the effects of lactation to increase osteoclast numbers and decrease trabecular bone volume, cortical thickness,...
Source: Journal of Clinical Investigation - June 25, 2019 Category: Biomedical Science Authors: Sutada Lotinun, Yoshihito Ishihara, Kenichi Nagano, Riku Kiviranta, Vincent T. Carpentier, Lynn Neff, Virginia Parkman, Noriko Ide, Dorothy Hu, Pamela Dann, Daniel Brooks, Mary L. Bouxsein, John Wysolmerski, Francesca Gori, Roland Baron Source Type: research

High multiplicity of infection following transplantation of hepatitis C virus–positive organs
Highly effective direct-acting antivirals against hepatitis C virus (HCV) have created an opportunity to transplant organs from HCV-positive individuals into HCV-negative recipients, since de novo infection can be routinely cured. As this procedure is performed more widely, it becomes increasingly important to understand the biological underpinnings of virus transmission, especially the multiplicity of infection. Here, we used single genome sequencing of plasma virus in 4 genotype 1a HCV-positive organ donors and their 7 organ recipients to assess the genetic bottleneck associated with HCV transmission following renal and ...
Source: Journal of Clinical Investigation - June 25, 2019 Category: Biomedical Science Authors: Muhammad N. Zahid, Shuyi Wang, Gerald H. Learn, Peter L. Abt, Emily A. Blumberg, Peter P. Reese, David S. Goldberg, George M. Shaw, Katharine J. Bar Source Type: research

Bypassing the bottleneck: intentional hepatitis C transmission with organ transplant
Solid organ transplantation from hepatitis C virus–positive (HCV-positive) deceased donors into HCV-negative recipients is a recent approach aimed to expand the donor organ pool in the setting of severe shortage. Good short-term outcomes have been reported with this approach in combination with direct-acting antivirals. In this issue of the JCI, Zahid and colleagues have characterized early viral kinetics and the genetic landscape of donor-to-recipient HCV transmission using single-genome sequencing. In seven HCV-negative recipients of four HCV-positive donor organs, productive infection with a highly diverse viral p...
Source: Journal of Clinical Investigation - June 25, 2019 Category: Biomedical Science Authors: Christine M. Durand, Michael A. Chattergoon Source Type: research

GSE101517 Gene expression analysis of human dermal fibroblasts - transcriptome profiling under normal versus induced DNA damage conditions
Contributors : Basuthkar J RAO ; Ishita S MEHTASeries Type : Expression profiling by arrayOrganism : Homo sapiensTo investigate genome-wide transcriptomic changes during CT relocation, we have employed whole genome microarray expression profiling as a discovery platform to identify differential gene expression changes aportioned per chromosome, which were associated during an induced and controlled DNA damage response paradigm. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE102499 Identification of the coiled-coil domain as an essential element of MBD3 for preserving lineage commitment potential of ESCs
Contributors : Masataka Hirasaki ; Yutaka Nakachi ; Yosuke Mizuno ; Yasushi Okazaki ; Akihiko OkudaSeries Type : Expression profiling by arrayOrganism : Mus musculusWe explored the effect of knockout of Mbd3 gene on global expression profile in ESCs and compared the ability to counteract such alteration among three isoforms of MBD3, i.e. MBD3a, b and c. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE102643 Identification of the coiled-coil domain as an essential element of MBD3 for preserving lineage commitment potential of ESCs
Contributors : Masataka Hirasaki ; Masakazu Kohda ; Yasushi Okazaki ; Akihiko OkudaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusAs one of approaches for elucidating the molecular bases which define the difference for counteracting defect of Mbd3-knockout ESCs between MBD3a mutant lacking MBD domain and that lacking coiled-coil domain, we explored the possibility that these two mutants are different in their ability to recruit PRC2 complex in close vicinity by ChIP-sequence analyses. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research

GSE116351 Analysis of Fusarium verticillioides gene expression in response to lactam and lactone compounds
Contributors : Gao Minglu ; Anthony E Glenn ; Scott E GoldSeries Type : Expression profiling by high throughput sequencingOrganism : Fusarium verticillioidesInvestigation of whole transcriptional changes in F. verticillioides FRC M-3125 when exposed to 5 μg/ml pyrrocidine A (PA), 20 μg/ml pyrrocidine B (PB), 50 μg/ml 2-benzoxazolinone (BOA), 50 μg/ml 2-oxindole (OXD), 50 μg/ml 2-coumaranone (CMN), or 50 μg/ml chlorzoxazone (CZX). Cultures were harvested one hour after exposure. Assessed in reference to control cultures of M-3125 exposed to D MSO (0.5% final concentration) since all the above compounds wer...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Fusarium verticillioides Source Type: research

GSE117442 Identification and Characterization of Splenic Adherent Cells forming Densely-Packed Colony
Contributors : Hirasaki Masataka ; Akihiko Okuda ; Norihiro KotaniSeries Type : Expression profiling by arrayOrganism : Mus musculusAs a first step for identifying hypothetical splenic stem cells for nonhematopoietic cells, densely-packed colony-forming cells were isolated from mouse spleen. Those which we designated as Splenic Adherent Colony-Forming Cells (SACCs) are positive for some of stem cell markers such as alkaline phosphatase and SSEA-1 antigen. We herewith determined global expression profiles of SACCs and control splenic adherent cells. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE117644 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: 3T12 deepCAGE
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusdeepCAGE was used in conjunction with Pacific Biosciences Iso-Seq and Illumina RNA-Seq to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE117645 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: Illumina RNA-Seq
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStrand-specific Illumina RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE117650 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: Iso-Seq
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPacific Biosciences Iso-Seq was used in conjunction with Illumina RNA-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE117651 Genome-wide transcript structure resolution reveals novel features of gammaherpesvirus transcription and yields an extensively revised murine gammaherpesvirus 68 genome annotation
Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE120051 H3K36 methylation and the chromodomain protein Eaf3 are required for proper co-transcriptional spliceosome assembly
Contributors : Calvin S Leung ; Stephen M Douglass ; Marco Morselli ; Matthew B Obusan ; Marat S Pavlyukov ; Matteo Pellegrini ; Tracy L JohnsonSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Saccharomyces cerevisiaeIn the eukaryotic cell, spliceosomes assemble onto pre-mRNA cotranscriptionally. The fact that spliceosome assembly takes place in the context of a dynamic chromatin environment suggests that the state of the chromatin may affect splicing. The molecular details and mechanisms through which chromatin regulates RNA splic...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Saccharomyces cerevisiae Source Type: research

GSE128499 Pioneer factor-nucleosome binding events during differentiation are motif-encoded
Contributors : Michael P Meers ; Derek H Janssens ; Steven HenikoffSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; OtherOrganism : Homo sapiensThough the in vitro structural and in vivo spatial characteristics of transcription factor (TF) binding are well defined, TF interactions with chromatin and other companion TFs during development are poorly understood. To analyze such interactions in vivo, we profiled several TFs across a time course of human embryonic stem cell differentiation via CUT&RUN epigenome profiling, and studied their interactions with nucleosomes and co-occurring TFs b...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Other Homo sapiens Source Type: research

GSE129259 Glucose metabolism drives epigenetic landscape transitions that dictate muscle stem cell function
We report the application of chromatin immunoprecpitation (ChIP) and assay for transposase accessible chromatin (ATAC) followed by sequencing to assay chromatin response to metabolic perturbation. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research

GSE129280 Muscle stem cell function
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research

GSE131197 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: HE 2.1 deepCAGE
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusdeepCAGE was used in conjunction with Pacific Biosciences Iso-Seq and Illumina RNA-Seq to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE131198 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: 3T12 BGI RNA-Seq
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStrand-specific RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE131199 Genome-wide transcript structure resolution in murine gammaherpesvirus 68: HE 2.1 BGI RNA-Seq
Contributors : Tina O'Grady ; April Feswick ; Brett Hoffman ; Yiping Wang ; Erik K Flemington ; Scott A TibbettsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStrand-specific RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE132294 Transcriptome of FliC-stimulated mouse bladder and dependency on TLR5
We report a transcriptional response in the mouse bladder following exposure to the major flagella filament protein, FliC. The response is shown to encompasses multiple innate immune networks not previously associated with FliC. Additionally, we provide analysis of components of this response that depend on the presence of the known flagellin receptor, TLR5. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE133191 Protein-coding changes preceded cis-regulatory gains in newly evolved transcription circuit
We describe an evolutionary pathway in fungi where a new transcriptional circuit (a-specific gene repression by Mat α2) evolved by coding changes in an ancient master regulator followed millions of years later by cis-regulatory sequence changes in the genes of its future regulon. We discerned this order of events by analyzing a group of species in which the coding changes in the regulator are present, but the ci s-regulatory changes in the target genes have not yet occurred. In this group we show that the coding changes became necessary for the regulator’s deeply conserved function and were therefore preserved....
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Wickerhamomyces anomalus Source Type: research

GSE133195 RNA-Seq analysis of wild-type and Hdac3-null E16.5 neurospheres and P0 cerebral cortex
Contributors : Lin Li ; Xiang-Jiao YangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo study the impact of cerebrum specific (Emx1-Cre) deletion of Hdac3 on embryonic neurospheres (cultured in vitro from E16.5 cerebrum) and neocortex from newborn pups (P0). (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE133203 Polymorphisms in RocA contribute to the molecular pathogenesis of serotype M28 group A streptococcus
Contributors : Paul E Bernard ; Randall J OlsenSeries Type : Expression profiling by high throughput sequencingOrganism : Streptococcus pyogenesRNA-seq experiments of serotype M28 group A streptococcus strains with mutations in rocA at two time points (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Streptococcus pyogenes Source Type: research

GSE133205 Gene expression in gastric Mist1+ cells.
Contributors : Masahiro Hata ; Hiroto Kinoshita ; Yoku Hayakawa ; Mitsuru Konishi ; Mayo Tsuboi ; Yukiko Oya ; Hayato Nakagawa ; Hiroaki Fujiwara ; Samuel Asfaha ; Daniel L Worthley ; Yuki Muranishi ; Takahisa Furukawa ; Shunsuke Kon ; Hiroyuki Tomita ; Timothy C Wang ; Kazuhiko KoikeSeries Type : Expression profiling by arrayOrganism : Mus musculusMist1+ cells and parietal cells in mouse stomach were separatedly sorted, and RNAs were isolated.Mist1 (also known as Bhlha15) is expressed in gastric chief cells and gastric stem cells in mice. However, more specific genes for each population needs to be identified to better un...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE133206 RNA-seq of human normal lung and lung cancer tissue
Contributors : Shin-ichiro Ohno ; Masahiko KurodaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn order to identify divergent transcripts involved in cancer development, next-generation sequencing analysis of RNA extracted from cancer and normal lung tissue was performed. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE133212 Conditional Activation of NF-kB Inducing Kinase (NIK) in the Osteolineage Enhances both Basal and Loading-Induced Bone Formation
Contributors : Jennifer L Davis ; Linda Cox ; Christine Shao ; Cheng Lyu ; Shaopeng Liu ; Rajeev Aurora ; Deborah J VeisSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStudies from global loss-of-function mutants suggest that alternative NF-kB downstream of NF-kB inducing kinase (NIK) is a cell-intrinsic negative regulator of osteogenesis. However, the interpretation of the osteoblast and/or osteocyte contribution to the bone phenotype is complicated by simultaneous osteoclast defects in these models. Therefore, we turned to a transgenic mouse model to investigate the direct role of N...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE133217 Differenatiation of miRNA expression in PBMCs (peripheral blood mononuclear cells)
Contributors : Caiyan Zhang ; Yufeng ZhouSeries Type : Non-coding RNA profiling by arrayOrganism : Homo sapiensTo determine miRNA transcribed during the PBMCs, we have employed whole genome microarray expression profiling as a discovery platform to identify miRNA expression in PBMCs donated by T1DM (type 1 diabetes mellitus) patients and healthy volunteers. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by array Homo sapiens Source Type: research

GSE133225 Differenatiation of ceRNA (circRNA, lncRNA and mRNA) expression in PBMCs (peripheral blood mononuclear cells)
Contributors : Caiyan Zhang ; Yufeng ZhouSeries Type : Expression profiling by array ; Non-coding RNA profiling by arrayOrganism : Homo sapiensTo determine ceRNA transcribed during the PBMCs, we have employed whole genome microarray expression profiling as a discovery platform to identify ceRNA expression in PBMCs donated by T1DM (type 1 diabetes mellitus) patients and healthy volunteers. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Non-coding RNA profiling by array Homo sapiens Source Type: research

GSE133226 A large-scale comparative study of isoform expressions measured on four platforms
Contributors : Rui Kuang ; Wei ZhangSeries Type : OtherOrganism : Homo sapiensThe NanoString experiment were conducted on 59 cell lines including 12 ovary cell lines, 12 lung cell lines, 11 colon cell lines, 10 breast cell lines, 4 pancreas cell lines, 2 prostate cell lines, 2 stomach cell lines, and 6 cell lines of 6 other types of tissues. A total of 403 probes were customized to estimate the isoform expressions of 155 cancer genes curated from the literature with more reliable isoform annotations, where each of the 155 gene contains at least two isoforms. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research

GSE133245 Next Generation Sequencing of Wild Type and Gata2-/- LSCs
Contributors : Juan B Gonzalez ; Neil Rodrigues ; Peter GilesSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGata2, a zinc finger TF, is essential for the generation and survival of HSCs in the embryo and has been implicated in the pathogenesis of AML, yet the requirement for Gata2 in adult HSCs and LSCs remains unclear. Using a conditional mouse model where Gata2 was deleted specifically in hematopoietic cells, we show that knockout of Gata2 leads to a rapid and complete cell-autonomous loss of adult HSCs. In Meis1a/Hoxa9 driven AML, deletion of Gata2 impedes maintenance and self-ren...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE133248 Next Generation Sequencing of Wild Type and Gata2+/- HSCs
Contributors : Ali Abdelfatah ; Neil Rodrigues ; Peter GilesSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGata2, a zinc finger TF, is essential for the generation and survival of HSCs in the embryo and has been implicated in the pathogenesis of AML, yet the requirement for Gata2 in adult HSCs and LSCs remains unclear. Using a conditional mouse model where Gata2 was deleted specifically in hematopoietic cells, we show that knockout of Gata2 leads to a rapid and complete cell-autonomous loss of adult HSCs. We then performed RNA-seq in sorted HSCs (LSK CD48- CD150+) from control and Ga...
Source: GEO: Gene Expression Omnibus - June 25, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

ROUTINE FUNDUS SCREENING OF FAMILIES OF CHILDREN WITH RETINOBLASTOMA: A Prospective Study of 131 Consecutive Families
Conclusion: Routine fundus screening of siblings allows for early detection of RB in otherwise asymptomatic children. Detection of spontaneously regressed RB in parents may act as a surrogate marker for germline RB1 mutation and is helpful in genetic counseling. (Source: RETINA)
Source: RETINA - June 25, 2019 Category: Opthalmology Tags: Original Study Source Type: research

First and second generation Probiotic therapeutics for Inflammatory Bowel Disease
Publication date: Available online 22 June 2019Source: PharmaNutritionAuthor(s): Srijita Sireswar, Ishita Ghosh, Gargi DeyAbstractThe interaction between the intestinal biota and humans have co-evolved, directing both to a state of complementary tolerance. However, dysbiosis in intestinal microbiota leads to inflammatory bowel disease which results from an aggressive immune response towards specific components of the commensal, enteric bacteria in a genetically susceptible host. One of the popular strategies for prophylaxis and treatment of inflammatory bowel disease has been the use of immune-regulatory probiotic formulat...
Source: PharmaNutrition - June 25, 2019 Category: Nutrition Source Type: research

Pediatric Langerhans cell histiocytosis: the impact of mutational profile on clinical progression and late sequelae
AbstractLangerhans cell histiocytosis (LCH) is a clonal histiocytic disorder with recurrent mutations ofBRAF andMAP2K1, but data on the impact of genetic features on progression and long-term sequelae are sparse. Cases of pediatric LCH with long-term follow-up from our institution were analyzed for mutations inBRAFV600 andMAP2K1 exons 2 and 3 by immunostaining with mutation-specific VE1 antibody, as well as allele-specific PCR and sequencing, respectively. Clinical and follow-up data were obtained from our files and a questionnaire sent to all former patients. Sixteen of 37 (43%) evaluable cases showedBRAFV600E, one case a...
Source: Annals of Hematology - June 25, 2019 Category: Hematology Source Type: research

The platelet NLRP3 inflammasome is upregulated in a murine model of pancreatic cancer and promotes platelet aggregation and tumor growth
AbstractPlatelets are activated in solid cancers, including pancreatic ductal adenocarcinoma (PDA), a highly aggressive malignancy with a devastating prognosis and limited therapeutic options. The mechanisms by which activated platelets regulate tumor progression are poorly understood. The nucleotide-binding domain leucine-rich repeat containing protein 3 (NLRP3) inflammasome is a key inflammatory mechanism recently identified in platelets, which controls platelet activation and aggregation. In an orthotopic PDA mouse model involving surgical implantation of Panc02 murine cancer cells into the tail of the pancreas, we show...
Source: Annals of Hematology - June 25, 2019 Category: Hematology Source Type: research

Korean Red Ginseng increases defective pol gene in peripheral blood mononuclear cells of HIV-1–infected patients; inhibition of its detection during ginseng-based combination therapy
ConclusionThe proportion of Δpol depends on template size as well as KRG treatment. HAART decreases the detection of Δpol. (Source: Journal of Ginseng Research)
Source: Journal of Ginseng Research - June 25, 2019 Category: Complementary Medicine Source Type: research

Association Between p.R4810K Variant and Long-Term Clinical Outcome in Patients With Moyamoya Disease
Conclusions: Compared to the patients in G/G group, patients in G/A+A/A group exhibited different clinical features, and had a lower rate of recurrent stroke and better clinical outcome after early medical and surgical interventions. Multivariate COX and logistic regression analysis showed that p.R4810Kvariant was not related to either recurrent stroke or neurological status. The p.R4810Kvariant may not be associated with long-term clinical outcome in Chinese patients with MMD. (Source: Frontiers in Neurology)
Source: Frontiers in Neurology - June 25, 2019 Category: Neurology Source Type: research

Linkage Evidence for a Two-Locus Inheritance of LQT-Associated Seizures in a Multigenerational LQT Family With a Novel KCNQ1 Loss-of-Function Mutation
Mutations in several genes encoding ion channels can cause the long-QT (LQT) syndrome with cardiac arrhythmias, syncope and sudden death. Recently, mutations in some of these genes were also identified to cause epileptic seizures in these patients, and the sudden unexplained death in epilepsy (SUDEP) was considered to be the pathologic overlap between the two clinical conditions. For LQT-associated KCNQ1 mutations, only few investigations reported the coincidence of cardiac dysfunction and epileptic seizures. Clinical, electrophysiological and genetic characterization of a large pedigree (n=241 family members) with LQT s...
Source: Frontiers in Neurology - June 25, 2019 Category: Neurology Source Type: research

An Update on Pediatric Cardiomyopathy
AbstractPurpose of reviewThis review summarizes the clinical characteristics and updated outcomes of primary pediatric cardiomyopathies including dilated (DCM), hypertrophic (HCM), and restrictive cardiomyopathy (RCM), and briefly discusses left ventricular non-compaction (LVNC) and arrhythmogenic cardiomyopathy (ACM), primarily arrythmogenic right ventricular cardiomyopathy (ARVC).Recent findingsPediatric cardiomyopathies are diseases of the heart muscle with an estimated annual incidence of 1.1 –1.5 cases per 100,000. They are progressive in nature and are frequently caused by a genetic mutation causing a structura...
Source: Current Treatment Options in Cardiovascular Medicine - June 25, 2019 Category: Cardiology Source Type: research

Multiple primary malignancies associated with a germline SMARCB1 pathogenic variant
AbstractA 51-year old presented with a 6-month history of increasing pelvic/lower back pain with nocturnal waking and episodes of anorexia and vomiting. Examination revealed right torticollis and Horner ’s syndrome, and a large abdominal mass arising from the pelvis. Magnetic resonance and positron emission tomography imaging revealed (A) a 14 cm heterogeneous enhancing mass, abutting the left kidney with standardised uptake value max = 2.9, (B) a large heterogeneous enhancing pelvic mass (C ) mesenteric adenopathy standardised uptake value max = 10.3 and (D) 6 cm right lung apex...
Source: Familial Cancer - June 25, 2019 Category: Cancer & Oncology Source Type: research

Radiomics: an Introductory Guide to What It May Foretell
AbstractPurpose of ReviewTo briefly review the radiomics concept, its applications, and challenges in oncology in the era of precision medicine.Recent FindingsOver the last 5  years, more than 500 studies have evaluated the role of radiomics to predict tumor diagnosis, genetic pattern, tumor response to therapy, and survival in multiple cancers. This new post-processing method is aimed at extracting multiple quantitative features from the image and converting them into mineable data.SummaryRadiomics models developed have shown promising results and may play a role in the near future in the daily patient management esp...
Source: Current Oncology Reports - June 25, 2019 Category: Cancer & Oncology Source Type: research

Cancers, Vol. 11, Pages 884: Loss of PTEN in Fallopian Tube Epithelium Results in Multicellular Tumor Spheroid Formation and Metastasis to the Ovary
In conclusion, loss of a single gene (PTEN) allows the fallopian tube epithelia to form MTS, which survive better under ultra-low adhesion conditions, attach to the extracellular matrix exposed during ovulation, and colonize the ovary. These results suggest that MTS may contribute to seeding of the ovary in HGSOC patients. (Source: Cancers)
Source: Cancers - June 25, 2019 Category: Cancer & Oncology Authors: Matthew Dean Vivian Jin Tova M. Bergsten Julia R. Austin Daniel D. Lantvit Angela Russo Joanna E. Burdette Tags: Article Source Type: research

Immunopathogenesis of Juvenile Systemic Sclerosis
Juvenile-onset systemic sclerosis (jSSc) is a rare and severe autoimmune disease with associated life-threatening organ inflammation and evidence of fibrosis. The organ manifestations of jSSc resemble adult SSc, but with better outcomes and survival. The etiology of jSSc appears treflect adult-onset SSc, with similar inflammatory mediators and autoantibodies, but with a significant population of children with uncharacterized anti-nuclear antibodies. The genetics of patients with jSSc differ from women with SSc, resembling instead the genes of adult males with SSc, with additional HLA genes uniquely associated with childhoo...
Source: Frontiers in Immunology - June 25, 2019 Category: Allergy & Immunology Source Type: research