Alterations in lipid metabolism accompanied by changes in protein and carotenoid content as spectroscopic markers of human T cell activation
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Apr 20:159496. doi: 10.1016/j.bbalip.2024.159496. Online ahead of print.ABSTRACTThis work aims to understand better the mechanism of cellular processes accompanying the activation of human T cells and to develop a novel, fast, label-free approach to identify molecular biomarkers for this process. Non-activated T-cell activation is a key method in cancer immunotherapy and involves the isolation of T-cells from a patient to perform a specific genetic modification. The standard methodology for confirming the activation state of T cells is based on flow cytometry, antibodies, and...
Source: Biochimica et Biophysica Acta - April 22, 2024 Category: Biochemistry Authors: Aleksandra Borek-Dorosz Anna Maria Nowakowska Paulina Laskowska Maciej Szyd łowski William Tipping Duncan Graham Katarzyna Wiktorska Przemyslaw Juszczynski Malgorzata Baranska Piotr Mrowka Katarzyna Majzner Source Type: research
TRPV3 promotes sebocyte inflammation via transcriptional modulating TLR2 in acne
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 20:167195. doi: 10.1016/j.bbadis.2024.167195. Online ahead of print.ABSTRACTAcne is a common chronic inflammatory disease of the pilosebaceous unit. Transient receptor potential vanilloid 3 (TRPV3) is an ion channel that is involved in inflammatory dermatosis development. However, the involvement of TRPV3 in acne-related inflammation remains unclear. Here, we used acne-like mice and human sebocytes to examine the role of TRPV3 in the development of acne. We found that TRPV3 expression increased in the skin lesions of Propionibacterium acnes (P. acnes)-injected acne-like mice and...
Source: Biochimica et Biophysica Acta - April 22, 2024 Category: Biochemistry Authors: Ziyu Wei Meng Gao Yihe Liu Rong Zeng Juan Liu Shuya Sun Siyuan Li Linghan Hu Ruiyu Xiang Ran Mo Zhongya Song Zhiming Chen Dan Bao Di Hua Christos C Zouboulis Yanyan Feng Ji Li Yong Yang Source Type: research
Unlocking the potential of adeno-associated virus in neuroscience: a brief review
Mol Biol Rep. 2024 Apr 22;51(1):563. doi: 10.1007/s11033-024-09521-6.ABSTRACTAdeno-associated virus (AAV) has emerged as a pivotal tool in neuroscience research, owing to its remarkable versatility and efficiency in delivering genetic material to diverse cell types within the nervous system. This mini review aims to underscore the advanced applications of AAV vectors in neuroscience and their profound potential to revolutionize our understanding of brain function and therapeutic interventions for neurological disorders. By providing a concise overview of the latest developments and strategies employing AAV vectors, this re...
Source: Molecular Biology Reports - April 22, 2024 Category: Molecular Biology Authors: Antea Minetti Source Type: research
Viewing early life without labels: optical approaches for imaging the early embryo
Biol Reprod. 2024 Apr 22:ioae062. doi: 10.1093/biolre/ioae062. Online ahead of print.ABSTRACTEmbryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo quality rely on subjective morphology assessments or an invasive biopsy for genetic testing. However, both approaches can be inherently inaccurate and crucially, fail to improve the live birth rate following the transfer of in vitro produced embryos. Optical imaging offers a potential non-invasive and accurate avenue for assessing embryo viability. Recent advances in various label-free ...
Source: Reproductive Biology - April 22, 2024 Category: Reproduction Medicine Authors: Darren J X Chow Tiffany C Y Tan Avinash Upadhya Megan Lim Kishan Dholakia Kylie R Dunning Source Type: research
Viewing early life without labels: optical approaches for imaging the early embryo
Biol Reprod. 2024 Apr 22:ioae062. doi: 10.1093/biolre/ioae062. Online ahead of print.ABSTRACTEmbryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo quality rely on subjective morphology assessments or an invasive biopsy for genetic testing. However, both approaches can be inherently inaccurate and crucially, fail to improve the live birth rate following the transfer of in vitro produced embryos. Optical imaging offers a potential non-invasive and accurate avenue for assessing embryo viability. Recent advances in various label-free ...
Source: Biology of Reproduction - April 22, 2024 Category: Reproduction Medicine Authors: Darren J X Chow Tiffany C Y Tan Avinash Upadhya Megan Lim Kishan Dholakia Kylie R Dunning Source Type: research
Kenya must wake up to the threat of cryptic Anopheles species and their impact on residual malaria transmission
This article discusses reports of these cryptic species, their importance to malaria transmission, especially in the arid and semi-arid areas, and what can be done to mitigate the situation.PMID:38648401 | DOI:10.4103/0972-9062.392264 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - April 22, 2024 Category: Infectious Diseases Authors: Eunice A Owino Source Type: research
Identification of ferroptosis-related key genes associated with immune infiltration in sepsis by bioinformatics analysis and in vivo validation
CONCLUSION: MAPK14 and ACSL4 could become robustly reliable and promising biomarkers for sepsis by regulating ferroptosis through the NLRP3 pathway, which is mainly associated with T-cell infiltration.PMID:38649061 | DOI:10.1016/j.gene.2024.148482 (Source: Gene)
Source: Gene - April 22, 2024 Category: Genetics & Stem Cells Authors: Rui Shi Chunyun Bai Shibo Sun Fang Wang Chaozhong Li Chongyu Wang Lidan Hu Ziwen Zhao Qiuzhe Guo Guanhua Du Dan Xu Alex F Chen Weimin Yang Source Type: research
Reflections on assortative mating, social stratification, and genetics
J Genet. 2024;103:15.ABSTRACTA recent report by G. Clark points to a sustained persistence of social status in England that extends vertically across several generations and horizontally across many levels of kinship. We seek to put his findings in historical perspective. We do so by relating them to two lines of thinking related to biological inheritance. One predated the rediscovery of Mendel's work and led to the field of quantitative genetics, which dealt on the whole with quasi-continuously varying traits. The other is based on the rediscovery itself and led to a reconciliation between quantitative genetics and discre...
Source: Journal of Genetics - April 22, 2024 Category: Genetics & Stem Cells Authors: Oliver Mayo Vidyanand Nanjundiah Source Type: research
Pan-Cancer Analysis of the Prognostic and Immunological Role of TOMM40 to Identify Its Function in Breast Cancer
In conclusion, we found that TOMM40 has prognostic value in 21 cancers, including breast cancer, by bioinformatics analysis. Based on immune correlation analysis, TOMM40 may also be a potential immunotherapeutic target for the treatment of BRCA. Therefore, our results may provide researchers to further explore the role of MARGs, especially TOMM40, in the developmental process of breast cancer, which may provide new directions and targets for the improvement of prognosis of breast cancer patients and their treatment.PMID:38649557 | DOI:10.1007/s10528-024-10794-6 (Source: Biochemical Genetics)
Source: Biochemical Genetics - April 22, 2024 Category: Genetics & Stem Cells Authors: Yaqing Zhou Xing Wei Lijun Jia Weimiao Li Shuqun Zhang Yonglin Zhao Source Type: research
A PAX3 insertion in the Celestial breed and certain feline breeding lines with dominant blue eyes
Anim Genet. 2024 Apr 22. doi: 10.1111/age.13433. Online ahead of print.ABSTRACTDuring the last 60 years many inherited traits in domestic outbred cats were selected and retained giving birth to new breeds characterised by singular coat or morphological phenotypes. Among them, minimal white spotting associated with blue eyes was selected by feline breeders to create the Altai, Topaz, and Celestial breeds. Various established breeds also introduced this trait in their lineages. The trait, that was confirmed as autosomal dominant by breeding data, was first described in domestic cats from Kazakhstan and Russia, in British sho...
Source: Animal Genetics - April 22, 2024 Category: Genetics & Stem Cells Authors: Marie Abitbol Alice Couronn é Caroline Dufaure de Citres Vincent Gache Source Type: research
A panel of tetranucleotide STR markers as an alternative approach to forensic DNA identification of wolf and dog
Anim Genet. 2024 Apr 21. doi: 10.1111/age.13428. Online ahead of print.ABSTRACTCommercial panels of microsatellite (STR) loci are focused on the use of DNA of the domestic dog (Canis lupus familiaris) and are often inapplicable for genotyping the DNA of the gray wolf (Canis lupus lupus). We propose a CPlex test system, including one hexa- and 12 tetranucleotide autosomal STR loci, as well as two sex loci, that is equally efficient in DNA identification of biological samples of the wolf and the dog. Analysis of molecular variance between samples revealed significant differentiation values (FST = 0.0784, p < 0.001), which...
Source: Animal Genetics - April 22, 2024 Category: Genetics & Stem Cells Authors: Aliaksandra E Hrebianchuk Nastassia S Parfionava Tatsiana V Zabauskaya Iosif S Tsybovsky Source Type: research
The role of apparent diffusion coefficient in the grading of adult isocitrate dehydrogenase-mutant astrocytomas: relationship with the Ki-67 proliferation index
CONCLUSION: Conventional MRI features and ADC are valuable to predict pathological grading of adult IDH-mutant astrocytomas.PMID:38644751 | DOI:10.1177/02841851241242653 (Source: Acta Radiologica)
Source: Acta Radiologica - April 22, 2024 Category: Radiology Authors: Yingxia She Xianwang Liu Jian Jiang Xuwen Wang Qian Niu Junlin Zhou Source Type: research
First Transcriptome Analysis of Hepatoblastoma in Brazil: Unraveling the Pivotal Role of Noncoding RNAs and Metabolic Pathways
In this study conducted in Brazilian patients, an in-depth whole transcriptome analysis was performed on 14 primary hepatoblastomas, compared to control liver tissues. The analysis unveiled 1,492 differentially expressed genes (1,031 upregulated and 461 downregulated), including 920 protein-coding genes (62%). Upregulated biological processes were linked to cell differentiation, signaling, morphogenesis, and development, involving known hepatoblastoma-associated genes (DLK1, MEG3, HDAC2, TET1, HMGA2, DKK1, DKK4), alongside with novel findings (GYNG4, CDH3, and TNFRSF19). Downregulated processes predominantly centered aroun...
Source: Biochemical Genetics - April 22, 2024 Category: Genetics & Stem Cells Authors: Talita Ferreira Marques Aguiar Maria Prates Rivas Edson Mario de Andrade Silva Sara Ferreira Pires Gustavo Dib Dangoni Taiany Curdulino Macedo Alexandre Defelicibus Bruna Dur ães de Figueiredo Barros Estela Novak Lilian Maria Cristofani Vicente Odone Mon Source Type: research
Identification of a Novel IGSF1 Variant in Two Malaysian Male Siblings with Central Hypothyroidism and Macroorchidism
J Clin Res Pediatr Endocrinol. 2024 Apr 22. doi: 10.4274/jcrpe.galenos.2024.2023-12-1. Online ahead of print.ABSTRACTIGSF1 mutation is the commonest cause of mild to moderate isolated central congenital hypothyroidism and has an X-linked recessive inheritance, primarily affecting males. Other notable clinical features are macroorchidism with delayed pubertal testosterone rise, large birth weight, increased body mass index, low prolactin, transient growth hormone deficiency and low prolactin. Two male siblings with central hypothyroidism were found to have a novel IGSF1 c.3467T>A variant that was likely pathogenic based ...
Source: JCRPE Journal of Clinical Research in Pediatric Endocrinology - April 22, 2024 Category: Endocrinology Authors: Yee Lin Lee Tzer Hwu Ting Chong Teik Lim Karuppiah Thilakavathy Nurul Huda Musa King Hwa Ling Source Type: research
Applications of L-Arginine in Pregnancy and Beyond: An Emerging Pharmacogenomic Approach
Curr Gene Ther. 2024 Apr 19. doi: 10.2174/0115665232262213240329034826. Online ahead of print.ABSTRACTL-arginine is a semi-essential amino acid that plays a critical role in various physiological processes, such as protein synthesis, wound healing, immune function, and cardiovascular regulation. The use of L-arginine in pregnancy has been an emerging topic in the field of pharmacogenomics. L-arginine, an amino acid, plays a crucial role in the production of nitric oxide, which is necessary for proper placental development and fetal growth. Studies have shown that L-arginine supplementation during pregnancy can have positiv...
Source: Current Gene Therapy - April 22, 2024 Category: Genetics & Stem Cells Authors: Sai Vaishnavi V Mahadevamma Lingaiah Bala Murali Mohan Sanku Sunil Kumar Kadri Manoj Kumar Mudigubba Source Type: research
