GSE262762 An immunohistochemical atlas of necroptotic pathway expression
Contributors : Andre L Samson ; James M Murphy ; Isabella Y KongSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusNecroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell death in vivo has proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis ...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE262864 Targeting an immunoregulatory dendritic cell-CCL22 program alleviates radioresistance
Conclusions: We identify a previously unappreciated cDC mechanism mediating Treg tumor infiltration after RT. Our findings suggest blocking the cDC1-CCL22-Treg axis augments RT efficacy. αEGFR-IFNα added to RT provided robust antitumor responses better than systemic free interferon administration, and may overcome clinical limitations to interferon therapy. Our findings highlight the complex behavior of cDC after RT and provide novel therapeutic strategies for overcoming RT-driven immunosuppression to improve RT efficacy. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE263369 Gene expression in kidney cells after unilateral ischemia-reperfusion
Contributors : Jamie R Privratsky ; Yanting ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo determine changes in gene expression due to ischemia-reperfusion AKI in different intra-renal cell populations (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE263829 Porphyrin overdrive rewires cancer cell metabolism
In conclusion, we identified a dependence of cancer cells on non-homeostatic heme metabolism, and we targeted this cancer metabolic vulnerability with a novel “bait-and-kill” strategy to eradicate malignant cells. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE263867 Heterogeneity of immune cells and their communications unveiled by transcriptome profiling in acute inflammatory lung injury
Conclusion: This study has identified specific gene signatures and signaling pathways in animal models and human samples that facilitate the interaction between immune cells, which could be targeted therapeutically in ARDS patients of various etiologies. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE263884 DNA Microarray and Bioinformatic Analysis Reveals the Potential of Whale Oil in Enhancing Hair Growth in C57BL/6 Mice
Contributors : Junko Shibato ; Randeep Rakwal ; Fumiko Takenoya ; Michio Yamashita ; Ai Kimura ; Satoshi Hirako ; Seiji ShiodaSeries Type : Expression profiling by arrayOrganism : Mus musculusMuch research has been conducted to determine how hair regeneration is regulated, as this could provide therapeutic, cosmetic, and even psychological interventions for hair loss. The current study focused on the hair growth effect and effective utilization of fatty oil obtained from Bryde's whales through high-throughput DNA microarray approach in conjunction with immunohistochemical observations. The research also examined the...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research
GSE263890 Combined bioinformatic and clinical assay uncovers resistance and susceptibility mechanisms of human glioblastomas to temozolomide and identifies new survival biomarkers outperforming MGMT promoter methylation
Contributors : Maria Suntsova ; Alexander Modestov ; Anton BuzdinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGlioblastoma (GBM) is the most aggressive and lethal CNS tumor with only 14 months median overall survival after diagnosis and ∼5% 5-years survival rate. The treatment strategy is mainly surgery and/or radiation therapy, both combined with adjuvant temozolomide (TMZ) chemotherapy. TMZ treatment results in methylation of DNA purine bases, where the primary cytotoxic lesion is O6-methylguanine that can be removed by DNA rep air enzyme methylguanine methyltransferase (MGM...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE263901 The Rapid Evolution of Chronic Inflammation to Tolerance / Immune Suppression: Mapping Bidirectional Checkpoints
Contributors : Elizabeth Mazzio ; Andrew BarnesSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCurrently, there is truly a universal lack of knowledge as to exactly how chronic inflammation leads to the initiation of cancer. Likewise, there exists an equal deficiency in knowledge to define the phenomenon of immune “ tolerance”. Tolerance is, however, generally described as the diminished biological response to repeated or sustained antigen exposure associated with limitation to efficacy of toll-like receptor (TLR) synthetic lipid A lipoglycans, or microbial antigen molecular pat...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE264058 Post-resolution macrophage-derived lipids shapes long-term tissue immunity and integrity
Contributors : Karen T Feehan ; Hannah E Bridgewater ; Jan Stenkiewicz-Witeska ; Roel P De Maeyer ; John Ferguson ; Matthias Mack ; Jemery Brown ; Giuseppe Ercoli ; Connar M Mawer ; Arne N Akbar ; James R Glanville ; Parinaaz Jalali ; Olivia V Bracken ; Anna Nicolaou ; Alexandra C Kendall ; Michelle A Sugimoto ; Derek W GilorySeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusResolving inflammation is thought to return the affected tissue back to homeostasis. However, we show that within days following resolution of Streptococcus pneumonia-triggered lung inflammation there is an i...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE264066 Effects of strigolactones on tomato leaf transcriptome under irrigated and repeated water stress conditions
This study investigated the positioning of strigolactones to the tomato flowering network, and their contribution to drought memory. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Solanum lycopersicum Source Type: research
GSE264084 Depletion of ribosome biogenesis factors in NPM1-mutant acute myeloid leukemia: Insights into direct consequences of the mutation and implications for treatment
Contributors : Aristi Damaskou ; Rachael Wilson ; Malgorzata Gozdecka ; Muxin Gu ; Maria Eleftheriou ; Eliza Yankova ; Justyna Rak ; George S VassiliouSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMutations in the NPM1 gene are found in more than 30% of acute myeloid leukemia (AML) cases. The mutations disrupt a nucleolar localization signal (NoLS) and create a novel nuclear export signal (NES), leading to cytoplasmic displacement of the protein (NPM1c). NPM1c mutations prime hematopoietic progenitors to leukemic transformation, but their precise molecular consequences remain...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE264166 shRNA-based miRNA overexpression results in significant off-target effects caused by isomiR production [miRNA-seq PC-3]
Contributors : Diana Maltseva ; Ivan Kirillov ; Anton Zhiyanov ; Roman Suvorov ; Daria Gubani ; Anna Kudriaeva ; Alexey Belogurov ; Alexander TonevitskySeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensmiRNA overexpression strategy based on RNA Polymerase (Pol) III mediated expression of short hairpin RNA (shRNA) coding particular miRNA is widely used for miRNA functional studies. For some miRNAs, e.g., encoded in the genome as a part of a polycistronic miRNA cluster, it is most likely the only way for their individual stable overexpression. Here we have revealed that extra ...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research
GSE264173 Insulin and leptin oscillations license food-entrained browning and metabolic flexibility [RNA-seq]
Contributor : P RajbhandariSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTimed-feeding leads to adipose browning, although the integrative mechanisms for the same remain unclear. Here we show that twice-a-nocturnal (TAN) feeding causes biphasic oscillations of circulating insulin and leptin, representing their entrainment by timed-feeding. Insulin and leptin surges lead to marked cellular, functional and metabolic remodeling of sWAT resulting in increased energy expenditure. Single-cell RNAseq analyses and flow cytometry reveal a role for insulin and leptin surges in ILC2 recruitme...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE254981 Auto-sumoylation of the Ubc9 E2 SUMO-conjugating Enzyme Extends Cellular Lifespan (RNA-Seq)
We report here that yeast adapted through in vitro evolution to the severe cellular stress caused by loss of the Ulp2 SUMO-specific protease exhibit both enhanced growth rates and replicative lifespan, and they have altered gene expression profiles similar to those observed in CR. Notably, in certain evolved ulp2 Δ lines, a dramatic increase in the auto-sumoylation of Ubc9 E2 SUMO-conjugating enzyme results in altered regulation of multiple targets involved in energy metabolism and translation at both transcriptional and post-translational levels. This increase is essential for the survival of aged cells an d CR-mediated ...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Saccharomyces cerevisiae Source Type: research
GSE255351 Prophage-encoded methyltransferase upregulates virulence to drive adaptation in an outbreak of community-acquired methicillin-resistant Staphylococcus aureus [Harm analysis]
Contributors : Robert J Ulrich ; Bo ShopsinSeries Type : Expression profiling by high throughput sequencingOrganism : Staphylococcus aureusTo determine the effects of phage m ⏀11 on gene expression in USA300 (LAC*) (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Staphylococcus aureus Source Type: research
