Association between < em > KRAS < /em > and < em > PIK3CA < /em > Mutations and Progesterone Resistance in Endometriotic Epithelial Cell Line
In this study, we investigated the effects of these mutations on progesterone resistance. Since the HMOsisEC10 had suppressed progesterone receptor (PR) expression, we transduced PR-B to HMOsisEc10 cell lines including KRAS mutant and PIK3CA mutant cell lines. We conducted a migration assay, invasion assay, and MTT assay using dienogest and medroxyprogestrone acetate. All cell lines showed progesterone sensitivity with or without mutations. Regarding inflammatory factors, real-time quantitative RT-PCR revealed that the KRAS mutation cell line exhibited no suppression of Cox-2 and mPGES-1 on progesterone treatment, whereas ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Kosuke Kanno Kentaro Nakayama Sultana Razia Sohel Hasibul Islam Zahan Umme Farzana Shahataj Begum Sonia Hitomi Yamashita Masako Ishikawa Tomoka Ishibashi Kayo Imamura Tohru Kiyono Satoru Kyo Source Type: research
Challenges in Defining a Reference Set of Differentially Expressed lncRNAs in Ulcerative Colitis by Meta-Analysis
Curr Issues Mol Biol. 2024 Apr 5;46(4):3164-3174. doi: 10.3390/cimb46040198.ABSTRACTThe study aimed to identify common differentially expressed lncRNAs from manually curated ulcerative colitis (UC) gene expression omnibus (GEO) datasets. Nine UC transcriptomic datasets of clearly annotated human colonic biopsies were included in the study. The datasets were manually curated to select active UC samples and controls. R packages geneknitR, gprofiler, clusterProfiler were used for gene symbol annotation. The R EdgeR package was used to analyze differential expression. This resulted in a total of nineteen lncRNAs that were diff...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Christopher G Fenton Mithlesh Kumar Ray Ruth H Paulssen Source Type: research
Frankincense ( < em > Boswellia serrata < /em > ) Extract Effects on Growth and Biofilm Formation of < em > Porphyromonas gingivalis < /em > , and Its Intracellular Infection in Human Gingival Epithelial Cells
Curr Issues Mol Biol. 2024 Mar 30;46(4):2991-3004. doi: 10.3390/cimb46040187.ABSTRACTFrankincense is produced by Boswellia trees, which can be found throughout the Middle East and parts of Africa and Asia. Boswellia serrata extract has been shown to have anti-cancer, anti-inflammatory, and antimicrobial effects. Periodontitis is an oral chronic inflammatory disease that affects nearly half of the US population. We investigated the antimicrobial effects of B. serrata extract on two oral pathogens associated with periodontitis. Using the minimum inhibitory concentration and crystal violet staining methods, we demonstrated th...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: David Vang Aline Cristina Abreu Moreira-Souza Nicholas Zusman German Moncada Harmony Matshik Dakafay Homer Asadi David M Ojcius Cassio Luiz Coutinho Almeida-da-Silva Source Type: research
The effectiveness of CA125 and HE4 as clinical prognostic markers in epithelial ovarian cancer patients with BRCA mutation
CONCLUSION: Normalizing both biomarkers emerged as the most effective predictive marker for the 1-year recurrence rate, regardless of BRCA mutational status. A negative HE4 value can be a useful predictor for 1-year recurrence-free survival in patients with BRCA mutations.PMID:38670560 | DOI:10.3802/jgo.2024.35.e80 (Source: Journal of Gynecologic Oncology)
Source: Journal of Gynecologic Oncology - April 26, 2024 Category: OBGYN Authors: Young Joo Lee Woojin Kim Soomin Hong Yong Jae Lee Jung-Yun Lee Sang Wun Kim Sunghoon Kim Young Tae Kim Eun Ji Nam Source Type: research
Tropomyosin 2 Regulates Tumor Cell Proliferation, Immune Suppression, and Activation of the JNK Signaling Pathway in Colitis-Associated Cancer (CAC)
CONCLUSIONS: Our results demonstrated that TPM2 knockdown suppressed the proliferation of CAC IECs, enhanced immune suppression on CAC IECs, and inhibited the JNK signaling pathway within the framework of CAC. These findings suggest TPM2 can serve as a potential therapeutic target for CAC treatment.PMID:38665026 | DOI:10.24976/Discov.Med.202436183.73 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Aixi Sun Jian Ge Kaixin Ding Zhiyang Gao Yun Zhang Source Type: research
Epithelial-Mesenchymal Transformation Promotes the Progression of Hepatocellular Carcinoma through NF- κB/MMP9 Axis
CONCLUSIONS: The NF-κB/MMP9 signaling axis serves as a pivotal regulator that fosters proliferation and impedes apoptosis in Huh7 cells by modulating the process of EMT.PMID:38665016 | DOI:10.24976/Discov.Med.202436183.63 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Jinsen Li Qingbin Cai Ziyue Wang Liangshi Wang Guangrun Yang Zhilong Zhang Na Ge Shunxin Hu Tian Bai Source Type: research
Cancers, Vol. 16, Pages 1682: BRCA1/2 Testing Landscape in Ovarian Cancer: A Nationwide, Real-World Data Study
da H. de Bock
Analyzing BRCA1/2 tumor pathogenic variants (TPVs) in epithelial tubal/ovarian cancers (EOCs) has become an essential part of the diagnostic workflow in many centers to guide treatment options and genetic cascade testing. However, there is no standardization of testing procedures, including techniques, gene assays, or sequencers used, and data on the execution of tumor tests remains scarce. Therefore, we evaluated characteristics of BRCA1/2 tumor testing in advanced-stage EOC with real-world national data. Pathology reports of patients diagnosed with EOC in 2019 in the Netherlands were obtained from the D...
Source: Cancers - April 26, 2024 Category: Cancer & Oncology Authors: Lieke Lanjouw Joost Bart Marian J. E. Mourits Stefan M. Willems Annemieke H. van der Hout Arja ter Elst Geertruida H. de Bock Tags: Article Source Type: research
Cancers, Vol. 16, Pages 1680: A Re-Examination of Neoadjuvant Therapy for Thymic Tumors: A Long and Winding Road
Mao
Wentao Fang
For most patients with advanced thymic epithelial tumors (TETs), a complete resection is a strong indicator of a better prognosis. But sometimes, primary surgery is unsatisfactory, and preoperative therapy is needed to facilitate complete resection. Neoadjuvant chemotherapy is the most used form of preoperative therapy. But studies on neoadjuvant chemotherapy have included mainly patients with thymoma; its efficacy in patients with thymic carcinoma is less known. Neoadjuvant chemoradiation has also been explored in a few studies. Novel therapies such as immunotherapy and targeted therapy have shown ef...
Source: Cancers - April 26, 2024 Category: Cancer & Oncology Authors: Fenghao Yu Zhitao Gu Xuefei Zhang Ning Xu Xiuxiu Hao Changlu Wang Yizhuo Zhao Teng Mao Wentao Fang Tags: Review Source Type: research
AKR1B10 accelerates glycolysis through binding HK2 to promote the malignant progression of oral squamous cell carcinoma
ConclusionTo sum up, AKR1B10 could bind to HK2 to accelerate glycolysis, thereby facilitating the proliferation, migration, invasion and EMT of OSCC cells. (Source: Hormones and Cancer)
Source: Hormones and Cancer - April 26, 2024 Category: Cancer & Oncology Source Type: research
IL-17 Control of Cutaneous Immune Homeostasis
IL-17 is widely recognized for its roles in host defense and inflammatory disorders. However, it has become clear that IL-17 is also an essential regulator of barrier tissue physiology. Steady-state microbe sensing at the skin surface induces low-level IL-17 expression that enhances epithelial integrity and resists pathogens without causing overt inflammation. Recent reports describe novel protective roles for IL-17 in wound healing and counteracting physiologic stress; however, chronic amplification of these beneficial responses contributes to skin pathologies as diverse as fibrosis, cancer, and autoinflammation. (Source:...
Source: Journal of Investigative Dermatology - April 26, 2024 Category: Dermatology Authors: Leah A. Johnston, Raji R. Nagalla, Mushi Li, Sarah K. Whitley Tags: Review Source Type: research
Epithelial-mesenchymal interaction protects normal colonocytes from 4-HNE-induced phenotypic transformation
ConclusionsDuring the exposure phase, the epithelial-mesenchymal interaction protected colonocytes from 4-HNE-induced phenotypic transformation via activation of the BMP pathway. This intercellular dialogue also limited the ability of fibroblasts to subsequently promote colonocyte-anchorage-independent growth. In contrast, fibroblasts pre-exposed to 4-HNE in monoculture strongly increased the ability of Co(m) colonocytes to form colonies. (Source: PLoS One)
Source: PLoS One - April 26, 2024 Category: Biomedical Science Authors: Jacques Dupuy Source Type: research
Exploring the impact of MiR-92a-3p on FOLFOX chemoresistance biomarker genes in colon cancer cell lines
Conclusion: We found that miR-92a-3p does not upregulate the expression of proteins of DNA-repair pathways and other genes involved in FOLFOX chemotherapy resistance.PMID:38659583 | PMC:PMC11039864 | DOI:10.3389/fphar.2024.1376638 (Source: Cancer Control)
Source: Cancer Control - April 25, 2024 Category: Cancer & Oncology Authors: Paula I Escalante Luis A Qui ñones H éctor R Contreras Source Type: research
Honokiol Suppresses Cell Proliferation and Tumor Migration through ROS in Human Anaplastic Thyroid Cancer Cells
CONCLUSION: Taken together, we provided the potential mechanism for treating ATC cells with honokiol, which significantly suppresses tumor proliferation and inhibits tumor metastasis in vitro through reactive oxygen species (ROS) induction.PMID:38659261 | DOI:10.2174/0118715303295608240408082523 (Source: Endocrine, Metabolic and Immune Disorders Drug Targets)
Source: Endocrine, Metabolic and Immune Disorders Drug Targets - April 25, 2024 Category: Endocrinology Authors: Kai-Sheng Liao Ying-Ray Lee Wen-Ying Chao Yen-Ju Huang Hui-Chen Chung Shu-Hsin Chen Yi-Zhen Li Pei-Wen Zhao Hong-Yi Chang Source Type: research
Retraction: Silencing of Prrx1b suppresses cellular proliferation, migration, invasion and epithelial-mesenchymal transition in triple-negative breast cancer
J Cell Mol Med. 2024 Apr;28(8):e18312. doi: 10.1111/jcmm.18312.ABSTRACTZhi-Dong Lv, Zhao-Chuan Yang, Xiang-Ping Liu, Li-Ying Jin, Qian Dong, Hui-Li Qu, Fu-Nian Li, Bin Kong, Jiao Sun, Jiao-Jiao Zhao, Hai-Bo Wang, Silencing of Prrx1b suppresses cellular proliferation, migration, invasion and epithelial-mesenchymal transition in triple-negative breast cancer. Journal of Cellular and Molecular Medicine, 20: 1640-1650. https://doi.org/10.1111/jcmm.12856 The above article, published online on 29 March 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-i...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Source Type: research
A Genuinely Hybrid, Multiscale 3D Cancer Invasion and Metastasis Modelling Framework
Bull Math Biol. 2024 Apr 25;86(6):64. doi: 10.1007/s11538-024-01286-0.ABSTRACTWe introduce in this paper substantial enhancements to a previously proposed hybrid multiscale cancer invasion modelling framework to better reflect the biological reality and dynamics of cancer. These model updates contribute to a more accurate representation of cancer dynamics, they provide deeper insights and enhance our predictive capabilities. Key updates include the integration of porous medium-like diffusion for the evolution of Epithelial-like Cancer Cells and other essential cellular constituents of the system, more realistic modelling o...
Source: Bulletin of Mathematical Biology - April 25, 2024 Category: Bioinformatics Authors: Dimitrios Katsaounis Nicholas Harbour Thomas Williams Mark Aj Chaplain Nikolaos Sfakianakis Source Type: research
