Electrospun EU/HPMC nanofibers decorated by ZIF-8 nanoparticle as the advanced electrochemical biosensor modifier for sensitive and selective detection of c-MET cancer biomarker in human plasma sample
Biosens Bioelectron. 2024 Apr 24;257:116319. doi: 10.1016/j.bios.2024.116319. Online ahead of print.ABSTRACTThis research presents a selective and sensitive electrochemical biosensor for the detection of the mesenchymal-epithelial transition factor (c-MET). The biosensing is based on a modification of the SPCE (screen-printed carbon electrode) with the electrospun nanofiber containing eudragit (EU), hydroxypropyl methylcellulose (HPMC), and Zeolite imidazolate frameworks (ZIF-8) nanoparticles. EU/HPMC/ZIF-8 nanofibers have presented a high capability of electron transfer, and more active surface area than bare SPCE due to ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Fatemeh Maleki Habib Razmi Mohammad-Reza Rashidi Mehdi Yousefi Soghra Ramezani Marjan Ghorbani Source Type: research
High-Grade Serous Ovarian Cancer during Pregnancy: From Diagnosis to Treatment
CONCLUSIONS: There have not been long-term follow-ups of patients' oncologic and obstetric outcomes. As patients should be comprehensively informed, more detailed case reports or series with longer follow-up periods are needed.PMID:38668047 | PMC:PMC11048790 | DOI:10.3390/curroncol31040144 (Source: Current Oncology)
Source: Current Oncology - April 26, 2024 Category: Cancer & Oncology Authors: Gregor Vivod Sebastjan Merlo Nina Kovacevic Source Type: research
Modulation of epithelial-mesenchymal transition by gemcitabine: Targeting ionizing radiation-induced cellular senescence in lung cancer cell
This study investigates the effects of ionizing radiation and gemcitabine on lung cancer cells, with a focus on induced senescence, EMT, and apoptosis. Human-derived A549, PC-9, and mouse-derived Lewis lung carcinoma cells exposed to 10 Gy X-ray irradiation exhibited senescence, as indicated by morphological changes, β-galactosidase staining, and cell cycle arrest through the p53-p21 pathway. Ionizing radiation also promoted EMT via TGFβ/SMAD signaling, evidenced by increased TGFβ1 levels, altered EMT marker expressions, and enhanced cell migration. Gemcitabine, a first-line lung cancer treatment, was shown to enhance a...
Source: Biochemical Pharmacology - April 26, 2024 Category: Drugs & Pharmacology Authors: Heng Zhou Chenghao Li Yanxian Ren Wen-An Wang Jiayuan Zhuang Yue Ren Lin Shen Yong Chen Source Type: research
Whole-exome sequencing reveals an association of rs112065068 in TGOLN2 gene with distant metastasis of non-small cell lung cancer
Gene. 2024 Apr 24:148507. doi: 10.1016/j.gene.2024.148507. Online ahead of print.ABSTRACTEarly prediction and prevention of recurring illness is critical for improving the survival rates of patients with non-small cell lung cancer (NSCLC). Previously, we demonstrated that the presence of premalignant epithelial changes in the small bronchi distant to the primary tumor is associated with NSCLC progression: isolated basal cell hyperplasia (iBCH) indicates a high risk of distant metastasis, BCH combined with squamous metaplasia (BCHSM) - a high risk of locoregional recurrence. Here, we aimed to identify germline single nucleo...
Source: Gene - April 26, 2024 Category: Genetics & Stem Cells Authors: Tatiana Gerashchenko Rostislav Skitchenko Anastasia Korobeynikova Kristina Kuanysheva Anna Khozyainova Rostislav Vorobiev Evgeny Rodionov Sergey Miller Evgeny Topolnitsky Nikolay Shefer Maxim Anisimenko Lilia Zhuikova Mrinal Vashisth Olga Pankova Vladimir Source Type: research
Profiling the molecular and clinical landscape of glioblastoma utilizing the Oncology Research Information Exchange Network brain cancer database
CONCLUSIONS: Herein, we report the first clinical, genomic, and transcriptomic analysis of ORIEN glioblastoma cases, incorporating sample reclassification under updated 2021 diagnostic criteria. These findings create multiple avenues for further investigation and reinforce the value of multi-institutional consortia such as ORIEN in deepening our knowledge of intractable diseases such as glioblastoma.PMID:38665799 | PMC:PMC11044707 | DOI:10.1093/noajnl/vdae046 (Source: Adv Data)
Source: Adv Data - April 26, 2024 Category: Epidemiology Authors: Alexandra N Demetriou Frances Chow David W Craig Michelle G Webb D Ryan Ormond James Battiste Arnab Chakravarti Howard Colman John L Villano Bryan P Schneider James K C Liu Michelle L Churchman Gabriel Zada Source Type: research
Association between < em > KRAS < /em > and < em > PIK3CA < /em > Mutations and Progesterone Resistance in Endometriotic Epithelial Cell Line
In this study, we investigated the effects of these mutations on progesterone resistance. Since the HMOsisEC10 had suppressed progesterone receptor (PR) expression, we transduced PR-B to HMOsisEc10 cell lines including KRAS mutant and PIK3CA mutant cell lines. We conducted a migration assay, invasion assay, and MTT assay using dienogest and medroxyprogestrone acetate. All cell lines showed progesterone sensitivity with or without mutations. Regarding inflammatory factors, real-time quantitative RT-PCR revealed that the KRAS mutation cell line exhibited no suppression of Cox-2 and mPGES-1 on progesterone treatment, whereas ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Kosuke Kanno Kentaro Nakayama Sultana Razia Sohel Hasibul Islam Zahan Umme Farzana Shahataj Begum Sonia Hitomi Yamashita Masako Ishikawa Tomoka Ishibashi Kayo Imamura Tohru Kiyono Satoru Kyo Source Type: research
Challenges in Defining a Reference Set of Differentially Expressed lncRNAs in Ulcerative Colitis by Meta-Analysis
Curr Issues Mol Biol. 2024 Apr 5;46(4):3164-3174. doi: 10.3390/cimb46040198.ABSTRACTThe study aimed to identify common differentially expressed lncRNAs from manually curated ulcerative colitis (UC) gene expression omnibus (GEO) datasets. Nine UC transcriptomic datasets of clearly annotated human colonic biopsies were included in the study. The datasets were manually curated to select active UC samples and controls. R packages geneknitR, gprofiler, clusterProfiler were used for gene symbol annotation. The R EdgeR package was used to analyze differential expression. This resulted in a total of nineteen lncRNAs that were diff...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Christopher G Fenton Mithlesh Kumar Ray Ruth H Paulssen Source Type: research
Frankincense ( < em > Boswellia serrata < /em > ) Extract Effects on Growth and Biofilm Formation of < em > Porphyromonas gingivalis < /em > , and Its Intracellular Infection in Human Gingival Epithelial Cells
Curr Issues Mol Biol. 2024 Mar 30;46(4):2991-3004. doi: 10.3390/cimb46040187.ABSTRACTFrankincense is produced by Boswellia trees, which can be found throughout the Middle East and parts of Africa and Asia. Boswellia serrata extract has been shown to have anti-cancer, anti-inflammatory, and antimicrobial effects. Periodontitis is an oral chronic inflammatory disease that affects nearly half of the US population. We investigated the antimicrobial effects of B. serrata extract on two oral pathogens associated with periodontitis. Using the minimum inhibitory concentration and crystal violet staining methods, we demonstrated th...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: David Vang Aline Cristina Abreu Moreira-Souza Nicholas Zusman German Moncada Harmony Matshik Dakafay Homer Asadi David M Ojcius Cassio Luiz Coutinho Almeida-da-Silva Source Type: research
The effectiveness of CA125 and HE4 as clinical prognostic markers in epithelial ovarian cancer patients with BRCA mutation
CONCLUSION: Normalizing both biomarkers emerged as the most effective predictive marker for the 1-year recurrence rate, regardless of BRCA mutational status. A negative HE4 value can be a useful predictor for 1-year recurrence-free survival in patients with BRCA mutations.PMID:38670560 | DOI:10.3802/jgo.2024.35.e80 (Source: Journal of Gynecologic Oncology)
Source: Journal of Gynecologic Oncology - April 26, 2024 Category: OBGYN Authors: Young Joo Lee Woojin Kim Soomin Hong Yong Jae Lee Jung-Yun Lee Sang Wun Kim Sunghoon Kim Young Tae Kim Eun Ji Nam Source Type: research
Tropomyosin 2 Regulates Tumor Cell Proliferation, Immune Suppression, and Activation of the JNK Signaling Pathway in Colitis-Associated Cancer (CAC)
CONCLUSIONS: Our results demonstrated that TPM2 knockdown suppressed the proliferation of CAC IECs, enhanced immune suppression on CAC IECs, and inhibited the JNK signaling pathway within the framework of CAC. These findings suggest TPM2 can serve as a potential therapeutic target for CAC treatment.PMID:38665026 | DOI:10.24976/Discov.Med.202436183.73 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Aixi Sun Jian Ge Kaixin Ding Zhiyang Gao Yun Zhang Source Type: research
Epithelial-Mesenchymal Transformation Promotes the Progression of Hepatocellular Carcinoma through NF- κB/MMP9 Axis
CONCLUSIONS: The NF-κB/MMP9 signaling axis serves as a pivotal regulator that fosters proliferation and impedes apoptosis in Huh7 cells by modulating the process of EMT.PMID:38665016 | DOI:10.24976/Discov.Med.202436183.63 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Jinsen Li Qingbin Cai Ziyue Wang Liangshi Wang Guangrun Yang Zhilong Zhang Na Ge Shunxin Hu Tian Bai Source Type: research
Electrospun EU/HPMC nanofibers decorated by ZIF-8 nanoparticle as the advanced electrochemical biosensor modifier for sensitive and selective detection of c-MET cancer biomarker in human plasma sample
Biosens Bioelectron. 2024 Apr 24;257:116319. doi: 10.1016/j.bios.2024.116319. Online ahead of print.ABSTRACTThis research presents a selective and sensitive electrochemical biosensor for the detection of the mesenchymal-epithelial transition factor (c-MET). The biosensing is based on a modification of the SPCE (screen-printed carbon electrode) with the electrospun nanofiber containing eudragit (EU), hydroxypropyl methylcellulose (HPMC), and Zeolite imidazolate frameworks (ZIF-8) nanoparticles. EU/HPMC/ZIF-8 nanofibers have presented a high capability of electron transfer, and more active surface area than bare SPCE due to ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Fatemeh Maleki Habib Razmi Mohammad-Reza Rashidi Mehdi Yousefi Soghra Ramezani Marjan Ghorbani Source Type: research
High-Grade Serous Ovarian Cancer during Pregnancy: From Diagnosis to Treatment
CONCLUSIONS: There have not been long-term follow-ups of patients' oncologic and obstetric outcomes. As patients should be comprehensively informed, more detailed case reports or series with longer follow-up periods are needed.PMID:38668047 | PMC:PMC11048790 | DOI:10.3390/curroncol31040144 (Source: Current Oncology)
Source: Current Oncology - April 26, 2024 Category: Cancer & Oncology Authors: Gregor Vivod Sebastjan Merlo Nina Kovacevic Source Type: research
Modulation of epithelial-mesenchymal transition by gemcitabine: Targeting ionizing radiation-induced cellular senescence in lung cancer cell
This study investigates the effects of ionizing radiation and gemcitabine on lung cancer cells, with a focus on induced senescence, EMT, and apoptosis. Human-derived A549, PC-9, and mouse-derived Lewis lung carcinoma cells exposed to 10 Gy X-ray irradiation exhibited senescence, as indicated by morphological changes, β-galactosidase staining, and cell cycle arrest through the p53-p21 pathway. Ionizing radiation also promoted EMT via TGFβ/SMAD signaling, evidenced by increased TGFβ1 levels, altered EMT marker expressions, and enhanced cell migration. Gemcitabine, a first-line lung cancer treatment, was shown to enhance a...
Source: Biochemical Pharmacology - April 26, 2024 Category: Drugs & Pharmacology Authors: Heng Zhou Chenghao Li Yanxian Ren Wen-An Wang Jiayuan Zhuang Yue Ren Lin Shen Yong Chen Source Type: research
Association between < em > KRAS < /em > and < em > PIK3CA < /em > Mutations and Progesterone Resistance in Endometriotic Epithelial Cell Line
In this study, we investigated the effects of these mutations on progesterone resistance. Since the HMOsisEC10 had suppressed progesterone receptor (PR) expression, we transduced PR-B to HMOsisEc10 cell lines including KRAS mutant and PIK3CA mutant cell lines. We conducted a migration assay, invasion assay, and MTT assay using dienogest and medroxyprogestrone acetate. All cell lines showed progesterone sensitivity with or without mutations. Regarding inflammatory factors, real-time quantitative RT-PCR revealed that the KRAS mutation cell line exhibited no suppression of Cox-2 and mPGES-1 on progesterone treatment, whereas ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Kosuke Kanno Kentaro Nakayama Sultana Razia Sohel Hasibul Islam Zahan Umme Farzana Shahataj Begum Sonia Hitomi Yamashita Masako Ishikawa Tomoka Ishibashi Kayo Imamura Tohru Kiyono Satoru Kyo Source Type: research
