N6-methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16-infected cervical squamous cell carcinoma
In this study, we observed that m6A regulators methyltransferase like protein (METTL14) and insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) were upregulated in HPV-positive CSCC tissues and cell lines, and their high expression predicted poor prognosis for HPV-infected CSCC patients. Cellular functional experiments verified that HPV16 oncogenes E6/E7 upregulated the expression of METTL14 and IGF2BP3 to promote cell proliferation and epithelial mesenchymal transition of CSCC cells. Next, we found that E6/E7 stabilized fascin actin-bundling protein 1 (FSCN1) mRNA and elevated FSCN1 expression in CSCC cells thro...
Source: Clinical and Experimental Pharmacology and Physiology - April 28, 2024 Category: Drugs & Pharmacology Authors: Qingqing Tian Juqing Huang Qin Zhang Jufen Zhao Source Type: research
LncRNA DDX11-AS1 promotes cell growth, migration and invasion through regulating epithelial-mesenchymal transition in breast cancer
This study aimed to investigate the expression of long non-coding ribonucleic acid (lncRNA) DDX11 antisense RNA 1 (DDX11-AS1) in breast cancer (BC) tissues and cells and investigate its biological function and potential molecular mechanism through in vitro experiments. Tissue specimens were obtained from 44 BC patients. TRIzol method was used to extract RNAs from the tissues. The relative expression of DDX11-AS1 in BC tissues and the expression of DDX11-AS1 in BC cells were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effect of DDX11-AS1 on the proliferation ability of BC cells w...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Xun Xi Fulan Yang Zhenluo Ding Haoxiang Zhuang Huozhong Yuan Source Type: research
LncRNA DDX11-AS1 promotes cell growth, migration and invasion through regulating epithelial-mesenchymal transition in breast cancer
This study aimed to investigate the expression of long non-coding ribonucleic acid (lncRNA) DDX11 antisense RNA 1 (DDX11-AS1) in breast cancer (BC) tissues and cells and investigate its biological function and potential molecular mechanism through in vitro experiments. Tissue specimens were obtained from 44 BC patients. TRIzol method was used to extract RNAs from the tissues. The relative expression of DDX11-AS1 in BC tissues and the expression of DDX11-AS1 in BC cells were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effect of DDX11-AS1 on the proliferation ability of BC cells w...
Source: Mol Biol Cell - April 28, 2024 Category: Molecular Biology Authors: Xun Xi Fulan Yang Zhenluo Ding Haoxiang Zhuang Huozhong Yuan Source Type: research
Effects of chronic stress on cancer development and the therapeutic prospects of adrenergic signaling regulation
Biomed Pharmacother. 2024 Apr 27;175:116609. doi: 10.1016/j.biopha.2024.116609. Online ahead of print.ABSTRACTLong-term chronic stress is an important factor in the poor prognosis of cancer patients. Chronic stress reduces the tissue infiltration of immune cells in the tumor microenvironment (TME) by continuously activating the adrenergic signaling, inhibits antitumor immune response and tumor cell apoptosis while also inducing epithelial-mesenchymal transition (EMT) and tumor angiogenesis, promoting tumor invasion and metastasis. This review first summarizes how adrenergic signaling activates intracellular signaling by bi...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 28, 2024 Category: Drugs & Pharmacology Authors: Hao Zhang Yuwei Yang Yan Cao Jingzhi Guan Source Type: research
N6-methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16-infected cervical squamous cell carcinoma
In this study, we observed that m6A regulators methyltransferase like protein (METTL14) and insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) were upregulated in HPV-positive CSCC tissues and cell lines, and their high expression predicted poor prognosis for HPV-infected CSCC patients. Cellular functional experiments verified that HPV16 oncogenes E6/E7 upregulated the expression of METTL14 and IGF2BP3 to promote cell proliferation and epithelial mesenchymal transition of CSCC cells. Next, we found that E6/E7 stabilized fascin actin-bundling protein 1 (FSCN1) mRNA and elevated FSCN1 expression in CSCC cells thro...
Source: Clinical and Experimental Pharmacology and Physiology - April 28, 2024 Category: Drugs & Pharmacology Authors: Qingqing Tian Juqing Huang Qin Zhang Jufen Zhao Source Type: research
LncRNA DDX11-AS1 promotes cell growth, migration and invasion through regulating epithelial-mesenchymal transition in breast cancer
This study aimed to investigate the expression of long non-coding ribonucleic acid (lncRNA) DDX11 antisense RNA 1 (DDX11-AS1) in breast cancer (BC) tissues and cells and investigate its biological function and potential molecular mechanism through in vitro experiments. Tissue specimens were obtained from 44 BC patients. TRIzol method was used to extract RNAs from the tissues. The relative expression of DDX11-AS1 in BC tissues and the expression of DDX11-AS1 in BC cells were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effect of DDX11-AS1 on the proliferation ability of BC cells w...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Xun Xi Fulan Yang Zhenluo Ding Haoxiang Zhuang Huozhong Yuan Source Type: research
Upregulated Matrisomal Proteins and Extracellular Matrix Mechanosignaling Underlie Obesity-Associated Promotion of Pancreatic Ductal Adenocarcinoma
Cancers (Basel). 2024 Apr 21;16(8):1593. doi: 10.3390/cancers16081593.ABSTRACTDiet-induced obesity (DIO) promotes pancreatic ductal adenocarcinoma (PDAC) in mice expressing KRasG12D in the pancreas (KC mice), but the precise mechanisms remain unclear. Here, we performed multiplex quantitative proteomic and phosphoproteomic analysis by liquid chromatography-tandem mass spectrometry and further bioinformatic and spatial analysis of pancreas tissues from control-fed versus DIO KC mice after 3, 6, and 9 months. Normal pancreatic parenchyma and associated proteins were steadily eliminated and the novel proteins, phosphoproteins...
Source: Cancer Control - April 27, 2024 Category: Cancer & Oncology Authors: Richard T Waldron Aurelia Lugea Hui-Hua Chang Hsin-Yuan Su Crystal Quiros Michael S Lewis Mingtian Che V Krishnan Ramanujan Enrique Rozengurt Guido Eibl Stephen J Pandol Source Type: research
Integrating integrins with the hallmarks of cancer
Matrix Biol. 2024 Apr 25:S0945-053X(24)00053-2. doi: 10.1016/j.matbio.2024.04.003. Online ahead of print.ABSTRACTEpithelial cells adhere to a specialized extracellular matrix called the basement membrane which allows them to polarize and form epithelial tissues. The extracellular matrix provides essential physical scaffolding and biochemical and biophysical cues required for tissue morphogenesis, differentiation, function, and homeostasis. Epithelial cell adhesion to the extracellular matrix (i.e., basement membrane) plays a critical role in organizing epithelial tissues, separating the epithelial cells from the stroma. Ep...
Source: Matrix Biology - April 27, 2024 Category: Molecular Biology Authors: Scott M Haake Brenda L Rios Ambra Pozzi Roy Zent Source Type: research
Integrating integrins with the hallmarks of cancer
Matrix Biol. 2024 Apr 25:S0945-053X(24)00053-2. doi: 10.1016/j.matbio.2024.04.003. Online ahead of print.ABSTRACTEpithelial cells adhere to a specialized extracellular matrix called the basement membrane which allows them to polarize and form epithelial tissues. The extracellular matrix provides essential physical scaffolding and biochemical and biophysical cues required for tissue morphogenesis, differentiation, function, and homeostasis. Epithelial cell adhesion to the extracellular matrix (i.e., basement membrane) plays a critical role in organizing epithelial tissues, separating the epithelial cells from the stroma. Ep...
Source: Matrix Biology - April 27, 2024 Category: Molecular Biology Authors: Scott M Haake Brenda L Rios Ambra Pozzi Roy Zent Source Type: research
Integrating integrins with the hallmarks of cancer
Matrix Biol. 2024 Apr 25:S0945-053X(24)00053-2. doi: 10.1016/j.matbio.2024.04.003. Online ahead of print.ABSTRACTEpithelial cells adhere to a specialized extracellular matrix called the basement membrane which allows them to polarize and form epithelial tissues. The extracellular matrix provides essential physical scaffolding and biochemical and biophysical cues required for tissue morphogenesis, differentiation, function, and homeostasis. Epithelial cell adhesion to the extracellular matrix (i.e., basement membrane) plays a critical role in organizing epithelial tissues, separating the epithelial cells from the stroma. Ep...
Source: Matrix Biology - April 27, 2024 Category: Molecular Biology Authors: Scott M Haake Brenda L Rios Ambra Pozzi Roy Zent Source Type: research
Integrating integrins with the hallmarks of cancer
Matrix Biol. 2024 Apr 25:S0945-053X(24)00053-2. doi: 10.1016/j.matbio.2024.04.003. Online ahead of print.ABSTRACTEpithelial cells adhere to a specialized extracellular matrix called the basement membrane which allows them to polarize and form epithelial tissues. The extracellular matrix provides essential physical scaffolding and biochemical and biophysical cues required for tissue morphogenesis, differentiation, function, and homeostasis. Epithelial cell adhesion to the extracellular matrix (i.e., basement membrane) plays a critical role in organizing epithelial tissues, separating the epithelial cells from the stroma. Ep...
Source: Matrix Biology - April 27, 2024 Category: Molecular Biology Authors: Scott M Haake Brenda L Rios Ambra Pozzi Roy Zent Source Type: research
Integrating integrins with the hallmarks of cancer
Matrix Biol. 2024 Apr 25:S0945-053X(24)00053-2. doi: 10.1016/j.matbio.2024.04.003. Online ahead of print.ABSTRACTEpithelial cells adhere to a specialized extracellular matrix called the basement membrane which allows them to polarize and form epithelial tissues. The extracellular matrix provides essential physical scaffolding and biochemical and biophysical cues required for tissue morphogenesis, differentiation, function, and homeostasis. Epithelial cell adhesion to the extracellular matrix (i.e., basement membrane) plays a critical role in organizing epithelial tissues, separating the epithelial cells from the stroma. Ep...
Source: Matrix Biology - April 27, 2024 Category: Molecular Biology Authors: Scott M Haake Brenda L Rios Ambra Pozzi Roy Zent Source Type: research
