Prospect of reprogramming replication licensing for cancer drug development.
Abstract Eukaryotic chromosomal DNA replication is preceded by replication licensing which involves the identification of the origin of replication by origin recognition complex (ORC). The ORC loads pre-replication complexes (pre-RCs) through a series of tightly regulated mechanisms where the ORC interacts with Cdc6 to recruit cdt1-MCM2-7 complexes to the origin of replication. In eukaryotes, adherence to regulatory mechanisms of the replication program is required to ensure that all daughter cells carry the exact copy of genetic material as the parent cell. Failure of which leads to the development of genome inst...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 23, 2021 Category: Drugs & Pharmacology Authors: Kyei Barffour I, Acheampong DO Tags: Biomed Pharmacother Source Type: research

Liposomes co-encapsulating doxorubicin and glucoevatromonoside derivative induce synergic cytotoxic response against breast cancer cell lines.
In this study, liposomes loaded with glucoevatromonoside containing peracetylated glucose hydroxyl groups (GEVPG) and DOX at molar ratio of 1:1 (SpHL-GEVPG:DOX 1:1) were developed, and their chemical and physicochemical properties were evaluated. This formulation presented a combination index (CI) lower than 1 at inhibitory concentration of 90 % growth (IC90) for three human breast tumor lines evaluated (0.52 ± 0.39 for MDA-MB-231, 0.19 ± 0.13 for MCF-7, and 0.99 ± 0.09 for SKBR-3). These results indicate a synergistic cytotoxic effect of the GEVPG and DOX combination encapsulated in liposomes. In addi...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Novais MVM, Gomes ER, Miranda MC, Silva JO, Gomes DA, Braga FC, Pádua RM, Oliveira MC Tags: Biomed Pharmacother Source Type: research

Structural stability of novel composite heart valve prostheses - Fatigue and wear performance.
Abstract Heart valve replacement is a very effective method to treat severe valvular stenosis or valvular insufficiency. The valve can be divided into the mechanical valve and biological valve according to the main materials of the valve leaflets. The former has good durability, but the patients need to take anticoagulants all their lives, otherwise, thrombosis will occur; the latter has good blood compatibility, and only 3-6 months of postoperative anticoagulation is required, but its durability is lower than the former. Compared with a traditional valve used materials, the fabric composite valve leaflets have bo...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Zhou H, Wu L, Wu Q Tags: Biomed Pharmacother Source Type: research

Longdan Xiegan Decoction alleviates experimental autoimmune uveitis in rats by inhibiting Notch signaling pathway activation and Th17 cell differentiation.
This study aimed to investigate the dynamic effects of the traditional Chinese medicine compound Longdan Xiegan Decoction (LXD) on the inhibition of Notch signaling pathway activation and T helper (Th) cell differentiation in rats with experimental autoimmune uveitis (EAU). Based on a network pharmacology strategy, we conducted protein interaction network analysis to construct an active ingredient-disease treatment network. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were further used to screen out the possible signaling pathways regulated by LXD in the treat...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Yin X, Qiu Y, Li Z, Guo L, Wei H, Liu B, Zhou M, Li T, Wang L, Jiang W, Bi H, Guo D Tags: Biomed Pharmacother Source Type: research

Resveratrol supplementation and acute pancreatitis: A comprehensive review.
Abstract Resveratrol, a natural polyphenolic ingredient extracted from herbs, suppresses oxidative stress and inflammation. We performed a comprehensive review to find any evidence about the effects of Resveratrol on acute pancreatitis (AP). Resveratrol has been found to directly impact cytokine generation. As these factors play a crucial role in the pathophysiology of AP, resveratrol might attenuate AP and its complications. Mechanistically, resveratrol exerts its pharmacological effects through anti-inflammatory and antioxidant mechanisms via interaction with different signaling molecules and transcription facto...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Agah S, Akbari A, Sadeghi E, Morvaridzadeh M, Basharat Z, Palmowski A, Heshmati J Tags: Biomed Pharmacother Source Type: research

Circular RNAs in depression: Biogenesis, function, expression, and therapeutic potential.
Abstract Depression is the second most common disease burden worldwide that threatens human health; however, mechanisms underlying the development of depression remain unclear. A family of non-coding RNAs, circular RNAs (circRNAs), has been shown to play a critical role in the development of depression by competitively binding to certain microRNAs (miRNA) and regulating the expression of target genes. Behavioral symptoms of depression may be ameliorated by knockdown or overexpression of depression-associated circRNAs. In this review, we summarized important functions of circRNAs and analyzed the most recent findin...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Gan H, Lei Y, Yuan N, Tang K, Hao W, Ma Q, Wu M, Zhou X, Li X, Huang J, Deng L, Chen J Tags: Biomed Pharmacother Source Type: research

Gemigliptin suppresses salivary dysfunction in streptozotocin-induced diabetic rats.
In this study, the protective roles of gemigliptin, a DPP4 inhibitor, against salivary dysfunction under diabetic conditions were investigated. Streptozotocin-induced diabetic rats received gemigliptin 10 mg/kg or 100 mg/kg via oral gavage for 3 weeks. The weights of salivary gland tissues, saliva secretion, and antioxidant capacity in salivary glands were reduced after diabetes induction, but were significantly preserved following gemigliptin treatment. In salivary gland analysis, expression of apoptotic proteins, as well as amylase and aquaporin-5 (AQP5) protein expression, were increased following gemigliptin treatment....
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Kang WS, Jung WK, Park SB, Kim HR, Kim J Tags: Biomed Pharmacother Source Type: research

The emerging role of non-coding RNAs in the regulation of PI3K/AKT pathway in the carcinogenesis process.
Abstract The PI3K/AKT pathway is an intracellular signaling pathway with an indispensable impact on cell cycle control. This pathway is functionally related with cell proliferation, cell survival, metabolism, and quiescence. The crucial role of this pathway in the development of cancer has offered this pathway as a target of novel anti-cancer treatments. Recent researches have demonstrated the role of microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) in controlling the PI3K/AKT pathway. Some miRNAs such as miR-155-5p, miR-328-3p, miR-125b-5p, miR-126, miR-331-3p and miR-16 inactivate this pathway, while miR-182...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 22, 2021 Category: Drugs & Pharmacology Authors: Ghafouri-Fard S, Abak A, Tondro Anamag F, Shoorei H, Majidpoor J, Taheri M Tags: Biomed Pharmacother Source Type: research

Nanotechnology shaping stem cell therapy: Recent advances, application, challenges, and future outlook.
Abstract Currently, stem cell nanotechnology is one of the novel and exciting fields. Certain experimental studies conducted on the interaction of stem cells with nanostructures or nanomaterials have made significant progress. The significance of nanostructures, nanotechnology, and nanomaterials in the development of stem cell-based therapies for degenerative diseases and injuries has been well established. Specifically, the structure and properties of nanomaterials affecting the propagation and differentiation of stem cells have become a new interdisciplinary frontier in material science and regeneration medicine...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 21, 2021 Category: Drugs & Pharmacology Authors: Dong Y, Wu X, Chen X, Zhou P, Xu F, Liang W Tags: Biomed Pharmacother Source Type: research

Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies.
CONCLUSION: The stem bark of Pentaclethra macrophylla possesses hepatoprotective effects that may result from its capacity to inhibit lipid peroxidation and could be attributed to its active components 3, 4 and 2. PMID: 33486213 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 21, 2021 Category: Drugs & Pharmacology Authors: Bomgning CLK, Sinda PVK, Ponou BK, Fotio AL, Tsague MK, Tsafack BT, Kühlborn J, Mbuyo-Nguelefack EP, Teponno RB, Opatz T, Tapondjou LA, Nguelefack TB Tags: Biomed Pharmacother Source Type: research

Effect of cultivation ages on anti-inflammatory activity of a new type of red ginseng.
Abstract Ginseng has been widely applied in clinical practice, but the cultivation age cannot be ignored as it influences the quality of ginseng and its products. In this work, different cultivation ages of fresh ginseng (FG) from four to seven years were analysed by UPLC-Q-TOF-MS/MS. Principal component analysis and supervised orthogonal partial least squared discrimination analysis, which belong to the normal method of multivariate statistical analysis, were applied to discover the characteristic components of FG at different cultivation ages. The components of new type of red ginseng (NRG) derived from FG at di...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Xu XF, Qu WJ, Jia Z, Han T, Liu MN, Bai YY, Wang M, Lin RC, Hua Q, Li XR Tags: Biomed Pharmacother Source Type: research

Crosstalk between PPARs and gut microbiota in NAFLD.
Abstract Nonalcoholic fatty liver disease (NAFLD) has become the most common liver disorder in both China and worldwide. It ranges from simple steatosis and progresses over time to nonalcoholic steatohepatitis (NASH), advanced liver fibrosis, cirrhosis, or hepatocellular carcinoma(HCC). Furthermore, NAFLD and its complications impose a huge health burden to society. The microbiota is widely connected and plays an active role in human physiology and pathology, and it is a hidden 'organ' in determining the state of the host, in terms of homeostasis, or disease. Peroxisome proliferator-activated receptors (PPARs) are...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wu L, Li J, Feng J, Ji J, Yu Q, Li Y, Zheng Y, Dai W, Wu J, Guo C Tags: Biomed Pharmacother Source Type: research

Cardioprotection of Panax Notoginseng saponins against acute myocardial infarction and heart failure through inducing autophagy.
Abstract Morbidity and mortality from acute myocardial infarction (AMI) remains substantial although interventional coronary reperfusion strategies are widely use and successful. MI remains the most common cause of heart failure (HF) worldwide. Here we demonstrated that Panax Notoginseng saponins (PNS), the extract of Panax notoginseng, exerts cardioprotective effect in AMI and the underlying mechanism refers to inducing cardiomyocyte autophagy, antiplatelet aggregation, enhancing endothelial migration and angiogenesis. PNS was initially tested to rescue the myocardial infarct size and cardiac function in left ant...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wang D, Lv L, Xu Y, Jiang K, Chen F, Qian J, Chen M, Liu G, Xiang Y Tags: Biomed Pharmacother Source Type: research

Chrysanthemum zawadskil Herbich attenuates dexamethasone-induced muscle atrophy through the regulation of proteostasis and mitochondrial function.
Abstract Chrysanthemum zawadskii Herbich (CZH) is used in traditional medicine to treat inflammatory diseases and diabetes. However, the effects of CZH on muscle wasting remains to be studied. Here, we investigated the effect of CZH on dexamethasone (DEX), a synthetic glucocorticoid, induced muscle atrophy. To examine the effect of CZH on muscle atrophy, C2C12 myotubes were co-treated with DEX and CZH for 24 h. The treatment with CZH prevented DEX-induced myotube atrophy in a dose-dependent manner. CZH inhibited the DEX-induced decrease of the MHC isoforms and the upregulation of atrogin-1 and MuRF1 in C2C12 diffe...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Lee H, Kim YI, Nirmala FS, Jeong HY, Seo HD, Ha TY, Jung CH, Ahn J Tags: Biomed Pharmacother Source Type: research

Repurposing of existing therapeutics to combat drug-resistant malaria.
Abstract In the era of drug repurposing, speedy discovery of new therapeutic options for the drug-resistant malaria is the best available tactic to reduce the financial load and time in the drug discovery process. Six anticancer drugs, three immunomodulators and four antibiotics were selected for the repositioning against experimental malaria owing to their mode of action and published literature. The efficacy of existing therapeutics was evaluated against chloroquine-resistant in vitro and in vivo strains of Plasmodium falciparum and P. yoelii, respectively. All the pre-existing FDA-approved drugs along with lept...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Yadav K, Shivahare R, Shaham SH, Joshi P, Sharma A, Tripathi R Tags: Biomed Pharmacother Source Type: research

Protective effect of grape seed extract and orlistat co-treatment against stroke: Effect on oxidative stress and energy failure.
Abstract Ischemic stroke is a major health concern and a leading cause of mortality worldwide. Oxidative stress is an early event in the course of stroke inducing neuro-inflammation and cell death. Grape seed extract (GSE) is a natural phytochemical mixture exhibiting antioxidant, anti-inflammatory and neuroprotective properties. Orlistat (ORL) is an anti-obesity agent and a gastro-intestinal lipase inhibitor which showed recently beneficial effects on brain lipotoxicity. Recent studies reported the increase of lipase activity upon stroke which led us to investigate the neuroprotective effect of ORL on rat brain I...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Kadri S, El Ayed M, Kadri A, Limam F, Aouani E, Mokni M Tags: Biomed Pharmacother Source Type: research

Anti-androgenetic alopecia effect of policosanol from Chinese wax by regulating abnormal hormone levels to suppress premature hair follicle entry into the regression phase.
In conclusion, policosanol prevents androgenetic alopecia by regulating hormone levels and suppressing premature hair follicle entry into the regression phase. PMID: 33485069 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wang ZD, Feng Y, Sun L, Gan J, Li X, Ding WF, Chen XM Tags: Biomed Pharmacother Source Type: research

Hydroxysafflor yellow A promotes apoptosis via blocking autophagic flux in liver cancer.
In this study, HSYA inhibited liver cancer cell growth in vivo and in vitro, evidenced by cell proliferation inhibition detected by CCK8, numerous apoptotic cells shown by flow cytometry assay, and expression of apoptosis-related proteins determined by western blot. Importantly, our data revealed that HSYA triggered autophagic response and autophagosome accumulation considering the increased levels of LC3II-conversion examined by western blot, LC3 puncta visualized by immunofluorescence, and expression of autophagy-related genes shown by quantitative real-time PCR. Furthermore, HSYA blocked the late-phase of autophagic flu...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wu N, Li J, Luo H, Wang D, Bai X Tags: Biomed Pharmacother Source Type: research

5-Fluorouracil (5-FU) resistance and the new strategy to enhance the sensitivity against cancer: Implication of DNA repair inhibition.
Abstract 5-Fluorouracil (5-FU) has been an important anti-cancer drug to date. With an increase in the knowledge of its mechanism of action, various treatment modalities have been developed over the past few decades to increase its anti-cancer activity. But drug resistance has greatly affected the clinical use of 5-FU. Overcoming this chemoresistance is a challenge due to the presence of cancer stem cells like cells, cancer recurrence, metastasis, and angiogenesis. In this review, we have systematically discussed the mechanism of 5-FU resistance and advent strategies to increase the sensitivity of 5-FU therapy inc...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Sethy C, Kundu CN Tags: Biomed Pharmacother Source Type: research

Combinatorial delivery of antigen and TLR agonists via PLGA nanoparticles modulates Leishmania major-infected-macrophages activation.
In conclusion, the findings of this study indicate that the addition of SLA in combination with TLR1/2 and TLR7/8 agonists either in NPs or in soluble forms overcome the suppression of L. major-infected macrophages. Moreover, encapsulation increases the strength and duration of the cytokines and iNOS expression, in parallel with decreasing parasite load, suggesting a longer availability or delivery of the NPs into the macrophages. These findings highlight the advantages of particulate therapeutic vaccine formulations. PMID: 33485119 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomede...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Katebi A, Varshochian R, Riazi-Rad F, Ganjalikhani-Hakemi M, Ajdary S Tags: Biomed Pharmacother Source Type: research

The new coumarin compound Bis 3 ameliorates cognitive disorder and suppresses brain-intestine-liver systematic oxidative stress in high-fat diet mice.
In this study, a 12-week high-fat diet model was established, and mice were randomly divided into 3 groups: standard diet, high-fat diet, and high-fat diet with Bis 3 treatment. Our results demonstrated that Bis 3 attenuated body weight gain and inhibited the development of insulin resistance in high-fat diet-fed mice. Bis 3 protected against high fat-induced intestinal barrier integrity damage and lipid content disorder. HFD-induced hepatocyte lipid metabolism disorder and hepatocyte damage were also alleviated by Bis 3. Moreover, the results of cognitive tests indicated that Bis 3 attenuated high fat-induced cerebral dys...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wang J, Zhang W, Li M, Li X Tags: Biomed Pharmacother Source Type: research

Environmental contaminant BPA causes intestinal damage by disrupting cellular repair and injury homeostasis in vivo and in vitro.
In this study, we established a mouse and intestinal epithelial cell model of BPA treatment to determine the underlying molecular mechanisms of BPA-induced intestinal injury. The results showed that the BPA treatment increased the intestinal permeability and disrupted the barrier function by increasing the chemical marker content and tight junction expression in intestinal tissues and blood circulation. BPA also altered the oxidative and antioxidant status of intestinal epithelial cells by increasing ROS and RNS contents and decreasing the activity levels of SOD, GPx, CAT, and T-AOC. BPA further induced inflammatory respon...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 20, 2021 Category: Drugs & Pharmacology Authors: Wang K, Qiu L, Zhu J, Sun Q, Qu W, Yu Y, Zhao Z, Yu Y, Shao G Tags: Biomed Pharmacother Source Type: research

Ozone inhalation induces exacerbation of eosinophilic airway inflammation and Th2-skew immune response in a rat model of AR.
CONCLUSIONS: O3 inhalation deteriorated symptoms in AR rats, and the possible mechanism is that ozone co-exposure could enhance the expression of Th2 cytokines, eosinophilic airway inflammation dose-dependently. The observation is helpful for us to understand the synergistic effect of O3 in the air pollution and allergen on aggravating allergic rhinitis. PMID: 33482509 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 19, 2021 Category: Drugs & Pharmacology Authors: Sun N, Niu Y, Zhang R, Huang Y, Wang J, Qiu W, Zhang X, Han Z, Bao J, Zhu H, Duan Y, Kan H Tags: Biomed Pharmacother Source Type: research

A novel amphibian-derived peptide alleviated ultraviolet B-induced photodamage in mice.
In this study, a new peptide (OM-GL15, GLLSGHYGRASPVAC) was identified from the skin of the green odorous frog (Odorrana margaretae). Results showed that OM-GL15 scavenged free radicals (2,2'-diazo-bis-3-ethylbenzothiazoline-6-sulfonic acid and 1,1-diphenyl-2-trinitrophenylhydrazine) and reduced Fe3+ to Fe2+. Moreover, topical administration of OM-GL15 significantly alleviated UVB-induced skin photodamage in mice. Exploration of the underlying mechanisms further showed that OM-GL15 exerted antioxidant potency. Specifically, the peptide reduced the levels of lipid peroxidation and malondialdehyde and protected epidermal cel...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 19, 2021 Category: Drugs & Pharmacology Authors: Zhang X, Feng C, Wang S, Wang Y, Fu Z, Zhang Y, Sun H, Xie C, Fu Y, Tao J, Luo M, Yang X Tags: Biomed Pharmacother Source Type: research

CB2 agonism controls pain and subchondral bone degeneration induced by mono-iodoacetate: Implications GPCR functional bias and tolerance development.
CONCLUSION AND IMPLICATIONS: Data presents analgesic and disease-modifying potential of CB2 agonists in OA treatment. Moreover, the study revealed more pronounced tolerance development for analgesic effects of the β-arrestin biased CB2 agonist GW833972A. These results provide a better understanding of the molecular underpinnings of the anti-nociceptive potential of CB2 agonists and may improve drug development processes for any cannabinoid-based chronic pain therapy. PMID: 33482616 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 19, 2021 Category: Drugs & Pharmacology Authors: Mlost J, Kostrzewa M, Borczyk M, Bryk M, Chwastek J, Korostyński M, Starowicz K Tags: Biomed Pharmacother Source Type: research

Diterpenoid anthraquinones as chemopreventive agents altered microRNA and transcriptome expressions in cancer cells.
CONCLUSION: This study revealed that CPT and DHT targeted cell proliferation and apoptosis signaling pathways via alterations in miRNAs and transcription factors. In addition, the findings of this study suggest that DHT is more potent than CPT in cancer chemopreventive activities. Therefore, DHT at a low dose is a TCM compound with less toxic side effects and may contribute to the development of natural medicine as a potential cancer chemopreventive agent. PMID: 33465676 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 16, 2021 Category: Drugs & Pharmacology Authors: Su YS, Kuo MZ, Kuo YT, Huang SW, Lee CJ, Su ZY, Ni YH, Li DK, Wu TY Tags: Biomed Pharmacother Source Type: research

H2S prevents peripheral immune cell invasion, increasing [Ca2+]i and excessive phagocytosis following hypoxia-ischemia injury in neonatal mice.
Abstract We previously reported that L-Cysteine, H2S donor, remarkably attenuated neuroinflammation following hypoxia-ischemia (HI) brain injury in neonatal mice. However, its anti-inflammatory mechanism for HI insult is still unknown. The study focus on the effects of L-Cysteine on immune cell populations, Ca2+ mobilization and phagocytosis after neonatal HI. We found that L-Cysteine treatment skewed CD11b+/CD45low microglia and CD11b+/CD45high brain monocytes/macrophages towards a more anti-inflammatory property 72 h after HI-injured brain. Moreover, L-Cysteine treatment reduced cerebral infiltration of CD4 T ce...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 15, 2021 Category: Drugs & Pharmacology Authors: Li T, Chu X, Xin D, Ke H, Wang S, Liu D, Chen W, Wang Z Tags: Biomed Pharmacother Source Type: research

Synergic effect of combined cyclosporin and melatonin protects the brain against acute ischemic reperfusion injury.
This study tested whether combined cyclosporin-A (CsA) and melatonin (Mel) was superior to either one on protecting the brain against ischemia (occluded left-middle-cerebral-artery for 90-min)-reperfusion (for 14 days) injury. METHODS AND RESULTS: Neuro-2a cells (N2a) were categorized into groups 1 (N2a), 2 (N2a-IR), 3 (N2a-IR-Mel), 4 (N2a-IR-CsA) and 5 (N2a-IR-CsA-Mel). in vitro results showed the protein expressions of cytosolic-cytochrome-C/mitochondrial-Bax/cleaved-capase-3/NOX-1/NOX-2 and flow-cytometric results of ROS (DCFDA/Mito-SOX) were highest in group 2, lowest in group 1, significantly lower in group 5 tha...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 15, 2021 Category: Drugs & Pharmacology Authors: Chen KH, Chai HT, Chen CH, Huang CR, Chiang JY, Sung PH, Chu YC, Yip HK Tags: Biomed Pharmacother Source Type: research

A strategy for component-based Chinese medicines design approach of Polygonum orientale L. against hypoxia/reoxygenation based on uniform design-stepwise regression-simulated annealing.
Abstract Presently, optimal proportions and synergistic mechanisms of component-based Chinese medicines are critical for developing novel strategies to treat cardiovascular diseases (CVDs). A new multi-objective optimization algorithm based on uniform design (UD) and stepwise regression (SR) modeling is proposed to find the synergistic effect of orientin (Ori), quercitrin (Que) and vitexin (Vit), the three effective components from Polygonum orientale L., using the H9c2 cells injury induced by hypoxia/reoxygenation (H/R). The optimal proportion of these three components was calculated by simulated annealing (SA). ...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 14, 2021 Category: Drugs & Pharmacology Authors: Wan S, Zhang J, Hou R, Zheng M, Liu L, Zhang M, Li Z, Huang X Tags: Biomed Pharmacother Source Type: research

Artesunate: A natural product-based immunomodulator involved in human complement.
In conclusion, we for the first time report the anti-complement bioactivities of ART and suggest a potential therapeutic benefit of ART in the complement-related human diseases. PMID: 33454596 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 14, 2021 Category: Drugs & Pharmacology Authors: Song L, Ge T, Li Z, Sun J, Li G, Sun Y, Fang L, Ma YJ, Garred P Tags: Biomed Pharmacother Source Type: research

A comprehensive review of anticancer mechanisms of action of Alantolactone.
Abstract Cancer is considered as the main challenge of human communities, and it annually imposes a significant economic burden on society. Natural products have been used for treatment of many diseases including inflammation, infections, neurological disorders, atherosclerosis, asthma and cancer for many years. Sesquiterpene lactones (STLs) refers to a group of natural products with different biological activities. A type of STL that has recently attracted much attention is Alantolactone (ALT). In recent years, many studies have investigated the molecular mechanism of this compound affecting cancer cells and resu...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 14, 2021 Category: Drugs & Pharmacology Authors: Babaei G, Gholizadeh-Ghaleh Aziz S, Rajabi Bazl M, Khadem Ansari MH Tags: Biomed Pharmacother Source Type: research

Non-coding RNAs modulate function of extracellular matrix proteins.
Abstract The extracellular matrix (ECM) creates a multifaceted system for the interaction of diverse structural proteins, matricellular molecules, proteoglycans, hyaluronan, and various glycoproteins that collaborate and bind with each other to produce a bioactive polymer. Alterations in the composition and configuration of ECM elements influence the cellular phenotype, thus participating in the pathogenesis of several human disorders. Recent studies indicate the crucial roles of non-coding RNAs in the modulation of ECM. Several miRNAs such as miR-21, miR-26, miR-19, miR-140, miR-29, miR-30, miR-133 have been dysr...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 14, 2021 Category: Drugs & Pharmacology Authors: Akbari Dilmaghnai N, Shoorei H, Sharifi G, Mohaqiq M, Majidpoor J, Dinger ME, Taheri M, Ghafouri-Fard S Tags: Biomed Pharmacother Source Type: research

CRISPR-Cas13a mediated targeting of hepatitis C virus internal-ribosomal entry site (IRES) as an effective antiviral strategy.
In conclusion, the CRISPR-Cas13a system efficiently targets HCV in vitro, suggesting its potential as a programmable therapeutic antiviral strategy. PMID: 33454599 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 14, 2021 Category: Drugs & Pharmacology Authors: Ashraf MU, Salman HM, Khalid MF, Khan MHF, Anwar S, Afzal S, Idrees M, Chaudhary SU Tags: Biomed Pharmacother Source Type: research

The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators.
Abstract Iron overload is increasingly implicated as a contributor to the pathogenesis of COVID-19. Indeed, several of the manifestations of COVID-19, such as inflammation, hypercoagulation, hyperferritinemia, and immune dysfunction are also reminiscent of iron overload. Although iron is essential for all living cells, free unbound iron, resulting from iron dysregulation and overload, is very reactive and potentially toxic due to its role in the generation of reactive oxygen species (ROS). ROS react with and damage cellular lipids, nucleic acids, and proteins, with consequent activation of either acute or chronic ...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 13, 2021 Category: Drugs & Pharmacology Authors: Habib HM, Ibrahim S, Zaim A, Ibrahim WH Tags: Biomed Pharmacother Source Type: research

EF24 induces ferroptosis in osteosarcoma cells through HMOX1.
CONCLUSION: Our results showed that EF24 upregulated HMOX1 to suppress GPX4 expression to induce ferroptosis by increasing MDA level, ROS level and intracellular ferric ion level. Thus, EF24 might serve as a potential agent for the treatment of HMOX1-positive osteosarcoma patients. PMID: 33453607 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 13, 2021 Category: Drugs & Pharmacology Authors: Lin H, Chen X, Zhang C, Yang T, Deng Z, Song Y, Huang L, Li F, Li Q, Lin S, Jin D Tags: Biomed Pharmacother Source Type: research

Role of peripheral 5-HT1D, 5-HT3 and 5-HT7 receptors in the mechanical allodynia induced by serotonin in mice.
This study also demonstrates the inhibitory role of peripheral 5-HT3 receptors in the nociceptive response induced by 5-HT or carrageenan. PMID: 33453675 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 13, 2021 Category: Drugs & Pharmacology Authors: Nascimento EB, Romero TRL, Dutra MMGB, Fiebich BL, Duarte IDG, Coelho MM Tags: Biomed Pharmacother Source Type: research

Agomelatine effects on fat-enriched diet induced neuroinflammation and depression-like behavior in rats.
In this study we postulated that in addition to improving mood, antidepressant therapy would reverse inflammatory changes in the brain of rats exposed to a HFD. To test our hypothesis, we measured the effect of the antidepressant agomelatine (AGO) on anxiety- and depressive-like behaviors, as well as on CNS markers of inflammation in rats rendered obese. Agomelatine is an agonist of the melatonin receptors MT1 and MT2 and an antagonist of the serotonin receptors 5HT2B and 5HT2C. A subset of rats was also treated with lipopolysaccharides (LPS) to determine how additional neuroinflammation alters behavior and affects the res...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 13, 2021 Category: Drugs & Pharmacology Authors: Rebai R, Jasmin L, Boudah A Tags: Biomed Pharmacother Source Type: research

Low-dose PPI to prevent bleeding after ESD: A multicenter randomized controlled study.
CONCLUSION: The intermittent use of low-dose PPIs is sufficient to prevent post-ESD bleeding. It might be applied in clinical practice to prevent post-ESD bleeding and reduce the costs related to PPIs. PMID: 33450486 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Yang L, Qi J, Chen W, Guo Q, Xie R, Zhao Z, Qin S, Liu A, Den M, Fan C, Bai J, Lin H, Guo H, Yang S Tags: Biomed Pharmacother Source Type: research

Ivabradine prevents deleterious effects of dopamine therapy in heart failure: No role for HCN4 overexpression.
Abstract BACKGROUND: Exacerbations of chronic heart failure (CHF) are often treated with catecholamines to provide short term inotropic support, but this strategy is associated with long-term detrimental hemodynamic effects and increased ventricular arrhythmias (VA), possibly related to increased heart rate (HR). We hypothesized that ivabradine may prevent adverse effects of short-term dopamine treatment in CHF. METHODS: Rats with post-myocardial infarction CHF received 2-week infusion of saline, dopamine(D), ivabradine(I) or D&I; cardiac function was assessed using echocardiography and pressure-volume lo...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Paterek A, Sochanowicz B, Oknińska M, Śmigielski W, Kruszewski M, Mackiewicz U, Mączewski M, Leszek P Tags: Biomed Pharmacother Source Type: research

Curcumin and cardiovascular diseases: Focus on cellular targets and cascades.
Abstract Cardiovascular diseases (CVDs) are one of the leading causes of the most considerable mortality globally, and it has been tried to find the molecular mechanisms and design new drugs that triggered the molecular target. Curcumin is the main ingredient of Curcuma longa (turmeric) that has been used in traditional medicine for treating several diseases for years. Numerous investigations have indicated the beneficial effect of Curcumin in modulating multiple signaling pathways involved in oxidative stress, inflammation, apoptosis, and proliferation. The cardiovascular protective effects of Curcumin against CV...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Pourbagher-Shahri AM, Farkhondeh T, Ashrafizadeh M, Talebi M, Samargahndian S Tags: Biomed Pharmacother Source Type: research

LncRNA influence sequential steps of hepatocellular carcinoma metastasis.
Abstract As a class of new and crucial molecules involved in the regulation of biological function, long noncoding RNA (lncRNA) have obtained widespread attention in recent days. While it was thought that lncRNA would be redundant in the past, it is proved that lncRNA identify a class of molecular that regulate the homeostasis including hepatocellular carcinoma in the present. All kinds of lncRNA have been implicated in a various of diseases, particularly in tumorigenesis and metastasis. But the mechanisms how they act is still not entirely clear. Metastasis is a major factor affecting long-term survival in hepato...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Lv E, Sheng J, Yu C, Rao D, Huang W Tags: Biomed Pharmacother Source Type: research

Muscone derivative ZM-32 inhibits breast tumor angiogenesis by suppressing HuR-mediated VEGF and MMP9 expression.
In this study, we synthesized five series of muscone derivatives and discovered that compound ZM-32 was effective in preventing HuR RRM1/2-Vegf-a mRNA complex formation. ZM-32 bound to HuR RRM1/2 protein with a KD value of 521.7 nmol/L. Furthermore, ZM-32 inhibited endothelial cell proliferation, migration, and tubule formation, and suppressed the VEGF/VEGFR2/ERK1/2 signaling axis mediated by macrophages in vitro. We also demonstrated that ZM-32 effectively prevented the proliferation and migration of breast cancer cells and inhibited the growth and angiogenesis of MDA-MB-231 xenograft tumors without any obvious toxicity i...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Yang LQ, Yu SP, Yang YT, Zhao YS, Wang FY, Chen Y, Li QH, Tian P, Zhu YY, Zhang JG, Lin GQ Tags: Biomed Pharmacother Source Type: research

Overexpression of human ATP-binding cassette transporter ABCG2 contributes to reducing the cytotoxicity of GSK1070916 in cancer cells.
Abstract The emergence of multidrug resistance (MDR) is one of the main factors that impair therapeutic outcome in cancer therapy. Among all the factors that contribute to MDR, overexpression of ABCG2 transporter has been described as a key factor. GSK1070916 is a potent Aurora kinase inhibitor with broad anticancer effects. The robust efficacy shown in preclinical studies allowed the drug progress to clinical investigation. However, the potential mechanisms of acquired resistance to GSK1070916 remain inconclusive. Since several Aurora kinase inhibitors were reported to be transported substrates of ABCG2, we aimed...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Wu ZX, Mai Q, Yang Y, Wang JQ, Ma H, Zeng L, Chen ZS, Pan Y Tags: Biomed Pharmacother Source Type: research

Persistent long-term platelet activation and endothelial perturbation in women with Takotsubo syndrome.
CONCLUSIONS: Our study highlighted the presence of endothelial perturbation in TTS patients even at long-term from the index event. The residual thromboxane production and platelet aggregation still leave open the question about the use of low dose ASA in this clinical setting. PMID: 33450492 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Amadio P, Porro B, Cavalca V, Barbieri SS, Eligini S, Fiorelli S, Di Minno A, Gorini A, Giuliani M, Werba JP, Cosentino N, Olivares P, Barbieri S, Veglia F, Tremoli E, Trabattoni D Tags: Biomed Pharmacother Source Type: research

Lung cancer chemotherapy using nanoparticles: Enhanced target ability of redox-responsive and pH-sensitive cisplatin prodrug and paclitaxel.
Abstract Platinum-based combination therapy is more effective and less toxic, but lack of targeting, and is not capable to enrich in the tumor zone. To obstacle these drawbacks, prodrug and nanotechnology strategies have been investigated in this study. GSH-responsive and pH-responsive cisplatin prodrug was synthesized. Cisplatin prodrug and paclitaxel co-loaded nanoparticles: DDP-P/PTX NPs were constructed. The drug release behavior and cytotoxicity of nanoparticles was assessed in vitro. In vivo anticancer efficiency and toxicity were evaluated on lung cancer bearing mice animal model. DDP-P/PTX NPs had a nanosc...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Wang B, Hu W, Yan H, Chen G, Zhang Y, Mao J, Wang L Tags: Biomed Pharmacother Source Type: research

Embryo-toxicity of docosahexaenoic and eicosapentaenoic acids: In vivo and in silico investigations using the chick embryo model.
CONCLUSION: According to the results of this study, one could confirm that the possible embryo-toxicity of omega-3 was approved by data presented in this research. The obtained results also support the suspicion that alteration of the apoptotic-related proteins in vessels is an essential pathway in embryo-toxicity of omega-3. PMID: 33450494 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Salari Z, Tavakkoli H, Khosravi A, Karamad E, Salarkia E, Ansari M, Dabiri S, Mortazaeizdeh A, Mosallanejad SS, Sharifi F Tags: Biomed Pharmacother Source Type: research

Copper nanoparticles: Biosynthesis, characterization, and protoscolicidal effects alone and combined with albendazole against hydatid cyst protoscoleces.
CONCLUSION: The findings of the present investigation showed potent protoscolicidal effects of CuNPs, especially combined with albendazole, which entirely eliminated the parasite after 10-20 min of exposure. The results also showed that although the possible protoscolicidal mechanisms of CuNPs are not clearly understood, the inducing apoptosis through caspases is one of the main protoscolicidal mechanisms of CuNPs. However, supplementary studies, especially in animal models and clinical settings, are needed to approve these results. PMID: 33450495 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacoth...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Ezzatkhah F, Khalaf AK, Mahmoudvand H Tags: Biomed Pharmacother Source Type: research

The influence of melatonin on the heart rhythm - An in vitro simulation with murine embryonic stem cell derived cardiomyocytes.
CONCLUSION: The study underlines the potential of this in vitro model to help explore the development of circadian rhythms and their modulation by melatonin in the embryonic phase. The results imply that melatonin influences the heart rhythm as early as during the embryonic heart development. Furthermore, the results indicate a potentially toxic effect of melatonin that has not been described in detail before. PMID: 33450496 [PubMed - as supplied by publisher] (Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie)
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Niehoff J, Matzkies M, Nguemo F, Hescheler J, Reppel M Tags: Biomed Pharmacother Source Type: research

Deoxidized gulose moiety attenuates the pulmonary toxicity of 6'-deoxy-bleomycin Z without effect on its antitumor activity.
Abstract Bleomycins (BLMs) are broad-spectrum antitumor drugs, but the dose-dependent lung toxicity has restricted their therapeutic applications. Many efforts have contributed to develop novel BLM analogues, but mainly focused on single functional domain owing to the structural complexity of BLM. Benefit from the engineered production of two novel analogues 6'-deoxy-BLM Z (6'-DO-BLM Z) and BLM Z, they together with clinical BLM-sulfate comprised a good model with varied sugar or C-terminal domain in any two of them, allowing us to study their structure-activity relationships pairwise. Our investigations suggested...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Kong J, Xiong Y, Duan Y, Zhu X Tags: Biomed Pharmacother Source Type: research

Investigation of the molecular mechanism underlying the inhibitory activities of ethanol extract of Bombyx mori pupa-incubated Cordyceps militaris fruiting bodies toward allergic rhinitis.
Abstract Cordyceps militaris has been widely studied for its various pharmacological activities such as antitumor, anti-inflammation, and immune regulation. The binding of an allergen to IgE-sensitized mast cells in nasal mucosa triggers allergic rhinitis. We found that oral administration of 300 mg/kg of the ethanol extract prepared from silkworm pupa-cultivated Cordyceps militaris fruiting bodies significantly alleviated the symptoms of ovalbumin-induced allergic rhinitis in mice, including sneeze/scratch, mast cell activation, eosinophil infiltration, and Syk activation. The treatment of ethanol extract signi...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 12, 2021 Category: Drugs & Pharmacology Authors: Wu TF, Shi WY, Chiu YC, Chan YY Tags: Biomed Pharmacother Source Type: research