MiR-325-3p inhibits renal inflammation and fibrosis by targeting CCL19 in diabetic nephropathy.
Abstract Diabetic nephropathy (DN), a common cardiovascular disease, has been a global health threat. MicroRNAs (miRNAs) havebeen proposed to frequently participate in the occurrence anddevelopment of DN. However, the role of miR-325-3p in DN remains uncharacterized.Our research aimed to explorethe function and mechanism of miR-325-3p in DN.Bioinformatics analysis (Targetscan, http://www.targetscan.org) and a wide range of experiments including RT-qPCR, CCK-8 assay, western blot, luciferase reporter assay, RIP assays, urine protein and blood glucose assays, histology analysis and morphometric analysis were used to...
Source: Clinical and Experimental Pharmacology and Physiology - June 30, 2020 Category: Drugs & Pharmacology Authors: Sun J, Wang J, Lu W, Xie L, Lv J, Li H, Yang S Tags: Clin Exp Pharmacol Physiol Source Type: research

Acute hypotensive effect of diminazene aceturate in Spontaneously Hypertensive Rats: role of NO and Mas receptor.
ll JG Abstract Diminazene aceturate (DIZE) has been described as an angiotensin-converting enzyme 2 (ACE2) activator. We aimed to investigate DIZE effects on blood pressure (BP) of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats. BP was recorded in awaked and unrestrained rats 24 h after femoral artery catheterization. DIZE (15 mg/kg, s.c.) produced a fast BP decrease only in SHR (P
Source: Clinical and Experimental Pharmacology and Physiology - June 30, 2020 Category: Drugs & Pharmacology Authors: Sartório CL, Pimentel EB, Dos Santos RL, Rouver WN, Mill JG Tags: Clin Exp Pharmacol Physiol Source Type: research

The dual orexin receptor antagonist daridorexant does not affect the pharmacokinetics of the BCRP substrate rosuvastatin.
nse J Abstract Daridorexant is a dual orexin receptor antagonist in clinical development for the treatment of insomnia. Breast-cancer resistant protein (BCRP) is an efflux pump expressed in intestinal epithelium and hepatocytes, contributing to the absorption, distribution, and elimination of drugs and endogenous compounds.In vitro, daridorexant inhibits BCRP with an IC50 of 3.0 μM. The BCRP substrate rosuvastatin is a cholesterol-lowering drug, recommended for clinical drug-drug interaction (DDI) studies. In order to exclude an inhibitory effect of daridorexant on BCRP, this single-centre, open-label, two-trea...
Source: Clinical and Experimental Pharmacology and Physiology - June 30, 2020 Category: Drugs & Pharmacology Authors: Zenklusen I, Muehlan C, Ulc I, Liška J, Dingemanse J Tags: Clin Exp Pharmacol Physiol Source Type: research

Survival motor neuron protein protects H9c2 cardiomyocytes from hypoxia-induced cell injury by reducing apoptosis.
CONCLUSION: SMN prevents hypoxia-induced cell apoptosis through inhibition of the mitochondrial apoptotic pathway, and thereby exerts a protective effect on H9c2 cardiomyocytes. PMID: 32603518 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - June 30, 2020 Category: Drugs & Pharmacology Authors: Zhong X, Song Z, Song X Tags: Clin Exp Pharmacol Physiol Source Type: research

Baseline, Delta, and Achieved Low-Density Lipoprotein Cholesterol Levels and Cardiovascular Risk in Patients on Statin Therapy: A Post-Hoc Resampling Mediation Analysis of Treating New Targets [TNT] Trial.
Abstract Clinical guidelines for monitoring low-density lipoprotein cholesterol (LDL-C) after statin therapy do not clearly define the clinical roles of baseline LDL-C, ΔLDL-C, and achieved LDL-C according to statin intensity. We performed post-hoc analysis of the Treating to New Target (TNT) study to evaluate individual LDL-C parameters after statin therapy. Primary outcome was the risk for total major adverse cardiovascular events (MACE). We use resampling multilevel mediation analysis to analyze complex relationships among LDL-C parameters based on similar statin intensities. Tertiles for resample A (matc...
Source: Clinical and Experimental Pharmacology and Physiology - June 25, 2020 Category: Drugs & Pharmacology Authors: Hyun MH, Won Jang J, Lee E, An H, Seog Seo H Tags: Clin Exp Pharmacol Physiol Source Type: research

A strategy for the Identification of Paracrine Regulators of Cancer Cell Migration.
Abstract We hypothesized that the correlation of the whole transcriptome with quantifiable phenotypes may unveil genes contributing to the regulation of the corresponding response. We tested this hypothesis in cultured fibroblasts exposed to diverse pharmacological and biological agents, to identify genes influencing chemoattraction of breast cancer cells. Our analyses revealed several genes that correlated, either positively or negatively with cell migration, suggesting that they may operate as activators or inhibitors of this process. Survey of the scientific literature showed that genes exhibiting positive or n...
Source: Clinical and Experimental Pharmacology and Physiology - June 25, 2020 Category: Drugs & Pharmacology Authors: Chavez B, Farmaki E, Zhang Y, Altomare D, Hao J, Soltnamohammadi E, Shtutman M, Chatzistamou I, Kiaris H Tags: Clin Exp Pharmacol Physiol Source Type: research

Antihypertensive effect of carvacrol is improved after incorporation in β-cyclodextrin as a drug delivery system.
Antihypertensive effect of carvacrol is improved after incorporation in β-cyclodextrin as a drug delivery system. Clin Exp Pharmacol Physiol. 2020 Jun 22;: Authors: Barreto L, Camargo SB, Dos Anjos Moraes R, Medeiros CF, de Melo Jesus A, Evangelista A, Villarreal CF, Quintans-Júnior LJ, Silva DF Abstract Carvacrol (CARV), has been shown to possess various pharmacological properties, especially in the treatment of cardiovascular diseases. We evaluated the antihypertensive effect of the CARV free and encapsulation of CARV in β-cyclodextrin (CARV/β-CD), and whether CARV/β-CD is...
Source: Clinical and Experimental Pharmacology and Physiology - June 22, 2020 Category: Drugs & Pharmacology Authors: Barreto L, Camargo SB, Dos Anjos Moraes R, Medeiros CF, de Melo Jesus A, Evangelista A, Villarreal CF, Quintans-Júnior LJ, Silva DF Tags: Clin Exp Pharmacol Physiol Source Type: research

SuPAR, a potential inflammatory mediator in psoriasis pathogenesis.
This study also compares the pattern of uPAR staining in healthy versus psoriatic skin. 39 psoriatic and 30 control subjects were included. Two biopsies (affected and unaffected skin) and one biopsy were taken from psoriasis patients and healthy controls respectively with uPAR staining of all skin biopsies. Blood samples from all subjects were obtained to determine suPAR, ESR, CRP, and fasting insulin levels. uPAR staining was prominent in unaffected skin from psoriasis patients and healthy individuals versus weak/absent uPAR staining in psoriatic skin. CRP, ESR and suPAR levels were not significantly elevated in the mild ...
Source: Clinical and Experimental Pharmacology and Physiology - June 19, 2020 Category: Drugs & Pharmacology Authors: Hamie L, Eid E, Abbas O, Safi R, Nammour T, Tamim H, Makki M, Stephan C, Hasbani D, Wehbe H, Ghaoui N, Hawa M, Nasser N, Eid A, Kibbi AG, Kurban M Tags: Clin Exp Pharmacol Physiol Source Type: research

Comparative studies of glial fibrillary acidic protein and brain-derived neurotrophic factor expression in two transgenic mouse models of Alzheimer's disease.
Abstract In Alzheimer's disease (AD)glial fibrillary acidic protein (GFAP) is expressed by reactive astrocytessurroundingβ-amyloid (Aβ) plaques, whereasbrain-derived neurotrophic factor (BDNF) levels are typically reduced. We compared the expression of GFAP, BDNF, and its precursor proBDNF in the dorsal hippocampus of two transgenicAD mouse models.APPSwe YAC mice expressing the APPSwe transgene on a yeast artificial chromosome (YAC) were assessed at age 4 and 21 months, and APPSwe/PS1dE9 miceco-expressingmutant amyloid precursor protein (APPSwe) and presenilin-1 (PS1dE9) were assessed at age 4 and 9 mont...
Source: Clinical and Experimental Pharmacology and Physiology - June 16, 2020 Category: Drugs & Pharmacology Authors: Edwards SR, Khan N, Coulson EJ, Smith MT Tags: Clin Exp Pharmacol Physiol Source Type: research

Sucralose causes non-selective CD4 and CD8 Lymphotoxicity via probable regulation of the MAPK8 / APTX / EID1 genes: an in vitro/in silico study.
This article presents the results of an evidence-based approach to the safety of human health in the use of Sucralose.Finally, this study points out that Sucralose has cytotoxic, genotoxic, and mutagenic effects in the concentrations and conditions tested in human lymphocyte cell culture. PMID: 32542867 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - June 16, 2020 Category: Drugs & Pharmacology Authors: Pasqualli T, Chaves PEE, da Veiga Pereira L, Serpa ÉA, de Oliveira LFS, Machado MM Tags: Clin Exp Pharmacol Physiol Source Type: research

"The pivotal relation between glucagon-like peptides,NF κB and inflammatory bowel disease".
"The pivotal relation between glucagon-like peptides,NFκB and inflammatory bowel disease". Clin Exp Pharmacol Physiol. 2020 Jun 08;: Authors: Azmy Nabeh O, Attallah MI, El-Gawhary NE Abstract Glucagon-like peptides (GLPs); GLP-1 and GLP-2, are released from intestinal enteroendocrine (L cells) in response to ingested nutrients. GLP-1 plays a crucial role in lowering blood glucose and controlling body weight, through stimulating the islet ß cells of pancreas to secret insulin, inhibiting gastric emptying, and reducing food ingestion. Therefore, GLP-1 receptor agonists are now used...
Source: Clinical and Experimental Pharmacology and Physiology - June 8, 2020 Category: Drugs & Pharmacology Authors: Azmy Nabeh O, Attallah MI, El-Gawhary NE Tags: Clin Exp Pharmacol Physiol Source Type: research

Immunodepression induced by influenza A virus (H1N1) in lymphoid organs functions as a pathogenic mechanism.
In this study, BALB/c female mice were infected with H1N1 by nasal inoculation for five days. After euthanasia, the brain, heart, lungs, thymus, liver, spleen, hilar lymph nodes, pancreas, kidneys, and adrenal glands were collected.Among these organs, only thelungs, thymus, spleen, and hilar lymph nodes showed lesions. Lung histopathology was characterized by widening of the septum, lymphocyte infiltration, alveolar effusion, and alveolar hyaline membrane formation. The thymus and spleen exhibited atrophy due to the apoptosis of numerous lymphocytes. Although the hilar lymph nodes were enlarged, lymphocyte apoptosis s...
Source: Clinical and Experimental Pharmacology and Physiology - June 4, 2020 Category: Drugs & Pharmacology Authors: Xiang L, Hu X, Zhang J, She J, Li M, Zhou T Tags: Clin Exp Pharmacol Physiol Source Type: research

Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand.
This study aimed to investigate the coordination of Fe(III) ions with a curcumin-oxime ligand that may be used in the treatment of iron overload. The synthesis of the curcumin-oxime ligand and curcumin-oxime-Fe(III) complex was successfully made and characterized in its solid-state and solution-state using FT-IR, UV-Vis, elemental analysis, and 1 H-NMR. However, in this study, we investigated the apoptotic effects of the curcumin-oxime Fe (III) complex on SW480. SW480 cells were exposed to 99.2% medium for 48 h. After the 48 h. the incubation period, cells were harvested by centrifug...
Source: Clinical and Experimental Pharmacology and Physiology - June 4, 2020 Category: Drugs & Pharmacology Authors: Özbolat G, Alizadeh Yegani A Tags: Clin Exp Pharmacol Physiol Source Type: research

"Utility of urinary organic anion transporter 5 as an early biomarker of obstructive nephropathy".
In this study, the Oat5 urinary excretion (Oat5u ) was evaluated as a potential biomarker of obstructive nephropathy, comparing it with traditional markers of renal function and with Neutrophil Gelatinase-Associated Lipocalin in urine (NGALu ), a more recent biomarker of renal pathology. Bilateral ureteral obstruction (BUO) was induced in male Wistar rats, by complete ligation of ureters for 1 h (BUO1), 2 h (BUO2), 5 h (BUO5), or 24 h (BUO24). After 24 h of ureteral releasing, urea and creatinine plasma concentrations, creatinine clearance, urinary total proteins, urinary glucose, and alkaline phosphatase activities in uri...
Source: Clinical and Experimental Pharmacology and Physiology - June 4, 2020 Category: Drugs & Pharmacology Authors: Campagno RV, Nosetto EC, Brandoni A, Torres AM Tags: Clin Exp Pharmacol Physiol Source Type: research

Salidroside protects against Diabetes Mellitus-induced kidney injury and renal fibrosis by attenuating TGF- β1 and Wnt1/3a/β-catenin signaling.
In conclusion, Salidroside protected against the kidney of rats from T1DM-induced injury and fibrosis by activating GS3Kβ-induced inhibition of Wnt1/Wnt3a β-catenin. This was associated with hypoglycemic and antioxidant effects. PMID: 32472701 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 30, 2020 Category: Drugs & Pharmacology Authors: Shati AA, Alfaifi MY, Shati AA Tags: Clin Exp Pharmacol Physiol Source Type: research

Genetic and phenotypic frequency distribution of CYP2C9, CYP2C19 and CYP2D6 in over 3200 Han Chinese.
CONCLUSIONS: Polymorphisms and phenotypic changes of CYP2C9, CYP2C19 and CYP2D6 are relatively frequent in the Han Chinese population. Thus, preemptive pharmacogenetic testing of CYP2C9, CYP2C19 and CYP2D6 should be recommended prior to dosing substrate drugs. PMID: 32469422 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 29, 2020 Category: Drugs & Pharmacology Authors: He L, Chen S, Li J, Xie X, Huang L, Kuang Y, Xu K, Huang W, Zhao Y, Yang G, Guo C Tags: Clin Exp Pharmacol Physiol Source Type: research

Tripartite motif 8 (TRIM8) plays a protective role in a cell model of Parkinson's disease via modulation of NF- κB activation through targeting of IκBα ubiquitination.
In conclusion, silencing of TRIM8 plays a protective role in the cell model of PD by inhibiting NF-κB activation through IκBα ubiquitination. PMID: 32463919 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 28, 2020 Category: Drugs & Pharmacology Authors: Pang Y, Rong Y, Zhu S, Pei H Tags: Clin Exp Pharmacol Physiol Source Type: research

A Role for Retinoids in The Treatment of Covid-19?
Abstract The 2020 global outbreak of the novel coronavirus (SARS-CoV-2 or Covid-19) is a serious threat to international health and thus there is an urgent need for discovery of novel therapies or use of repurposed drugs that can make a significant impact on slowing the spread of the virus. Type 1 interferons (IFN-I) are a family cytokines of the early innate immune response to viruses that are being tested against SARS-CoV-2. However coronaviruses similar to SARS-CoV-2 can suppress host IFN-I anti-viral responses. Retinoids are a family molecules related to vitamin A that possess robust immune modulating properti...
Source: Clinical and Experimental Pharmacology and Physiology - May 27, 2020 Category: Drugs & Pharmacology Authors: Trasino SE Tags: Clin Exp Pharmacol Physiol Source Type: research

Assessment of heavy metals by ICP-OES and their impact on insulin stimulating hormone and carbohydrate metabolizing enzymes.
Abstract Arsenic (As) and cadmium (Cd) have been recently emerged as major health concerns owing to their strong association with diabetes mellitus (DM). We aimed to investigate the heavy metals exposure towards incidence of DM at various enzymatic and hormonal levels. Additionally, association of As and Cd with Zinc (essential metal) was also evaluated. Spot urine samples were collected to assess As, Cd and Zn through ICP-OES. Serum was analyzed by assay method for fasting blood glucose, liver and renal function biomarkers. ELISA was performed to investigate the impact of heavy metals on HbA1c, α-amylase, D...
Source: Clinical and Experimental Pharmacology and Physiology - May 20, 2020 Category: Drugs & Pharmacology Authors: Sabir S, Akash MSH, Rehman K, Saleem U, Fiayyaz F, Ahmad T Tags: Clin Exp Pharmacol Physiol Source Type: research

Donepezil down-regulates propionylation, 2-hydroxyisobutyrylation, butyrylation, succinylation, and crotonylation in the brain of bilateral common carotid artery occlusion-induced vascular dementia rats.
In this study, we evaluated the effects of donepezil on VaD, and investigated the underlying molecular mechanisms of action. VaD was established by ligation of the bilateral common carotid artery occlusion (BCCAO). Executive function was tested by the Morris Water Maze (MWM) test and the attentional set shifting task (ASST). Our results showed that donepezil improved executive dysfunction and cognitive flexibility in BCCAO rats. In addition, we showed that donepezil treatment decreased the level of Aβ1-42 in BCCAO rats by enzyme-linked immunosorbent assay. Posttranslational modifications (PTMs) are known to be critica...
Source: Clinical and Experimental Pharmacology and Physiology - May 18, 2020 Category: Drugs & Pharmacology Authors: Wang H, Lu J, Gao WC, Ma X, Li N, Ding Z, Wu C, Zhu M, Qiao G, Xiao C, Zhang C, Chen C, Weng Z, Yang W, Zheng CB Tags: Clin Exp Pharmacol Physiol Source Type: research

Biochemical investigation of rs1801282 variations in PPAR- γ gene and its correlation with risk factors of diabetes mellitus in coronary artery disease.
This study suggests that hyperglycemia in CAD-patients particularly obese, smokers and hypertensives having Pro/Ala polymorphism in PPAR-γ gene are at high risk of developing DM as clearly observed by hyperglycemia in CAD-patients. PMID: 32416637 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 16, 2020 Category: Drugs & Pharmacology Authors: Rehman K, Jabeen K, Awan FR, Hussain M, Saddique MA, Akash MSH Tags: Clin Exp Pharmacol Physiol Source Type: research

The anti-tumorigenic effect of Ellagic acid in SKOV-3 ovarian cancer cells entails activation of autophagy-mediated by inhibiting Akt and activating AMPK.
In conclusion, EA can inhibit SKOV-3 growth, migration, and invasion by activating cytotoxic autophagy mediated by inhibition of mTORC1 and Akt and activation of AMPK. PMID: 32415699 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 15, 2020 Category: Drugs & Pharmacology Authors: Elsaid FG, Alshehri MA, Shati AA, Al-Kahtani MA, Alsheri AS, Massoud EE, El-Kott AF, El-Mekkawy HI, Al-Ramlawy AM, Abdraboh ME Tags: Clin Exp Pharmacol Physiol Source Type: research

Estimates of beta cell function adjusted by anthropometric markers in patients with T2DM.
Abstract We sought to determine whether adjusting the indices used to assess beta cell function by anthropometric markers of obesity improves their clinical value in a diabetic population. We conducted a cross-sectional survey of 3,732 diabetic patients who underwent a 100 gram carbohydrate-meal test. Insulin secretion was estimated using HOMA-B of steady state as well as △C0-30 /△G0-30 , △AUCc30-120 /△AUCG30-120 and CPIn for dynamic state. Body weight index, waist circumference, waist-hip ratio and body surface area were recorded. The final analysis included 2,873 T2DM patients. Correlation analyses showe...
Source: Clinical and Experimental Pharmacology and Physiology - May 15, 2020 Category: Drugs & Pharmacology Authors: Zhang M, Su Y, Quan L, Jiang S, Yao H Tags: Clin Exp Pharmacol Physiol Source Type: research

ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human Periodontal Ligament Fibroblasts via Rac1/Cdc42.
This study highlights the importance of Rho signaling in the response of human PDL fibroblasts to mechanical stress. PMID: 32391922 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - May 11, 2020 Category: Drugs & Pharmacology Authors: Wang L, Yang X, Wan L, Wang S, Liu Y, Pan J Tags: Clin Exp Pharmacol Physiol Source Type: research

Toll-like receptor-2 mediates systemic inflammation in gentamicin-induced rat nephrotoxicity.
Abstract Gentamicin is an aminoglycoside antibiotic commonly administrated to patients with gram-negative infections. Gentamicin induced nephrotoxicity by functional and structural impairment. Toll like receptors (TLRs) as key mediators in the innate and adaptive immune system response involved in gentamicin induced-nephrotoxicity. The present study aimed to investigate the gene expression of TLR2 and pro-inflammatory cytokines in the renal tissues and buffy coat of the whole blood in gentamicin treated rats. Twenty adult male Sprague Dawley rats weighting 180-200 were randomly divided into gentamicin (100 mg/kg, ...
Source: Clinical and Experimental Pharmacology and Physiology - May 8, 2020 Category: Drugs & Pharmacology Authors: Karimi Z, Pakfetrat Z, Roozbeh J, Janfeshan S Tags: Clin Exp Pharmacol Physiol Source Type: research

Tobramycin suppresses HUWE1 degradation to control MCL-1 stability during tumour development.
Abstract HUWE1 is an E3 ubiquitin ligase that is involved in cancer cell proliferation by regulating MCL-1 stability. The HECT domain has been shown to be required for the ubiquitin ligase activity of HUWE1. To identify efficient drugs that impairthe activity of HUWE1, and thus decrease MCL-1 accumulation, we screened 2000 candidate compounds that might suppress HUWE1 activity. To evaluate these 2000 candidates, the HECT domain of HUWE1, which is the catalytic domain responsible for MCL1 ubiquitination, was selected as a conjugation site, and putative binding candidates were filtrated. Tobramycin emerged as one of...
Source: Clinical and Experimental Pharmacology and Physiology - May 7, 2020 Category: Drugs & Pharmacology Authors: Jiabei H, Sixian W, Li X, Li T, Hanxiao C, Lang Q, Jiang X, Tao L, Wenming X Tags: Clin Exp Pharmacol Physiol Source Type: research

Low levels of salicylic acid and salicyluric acid are present in synovial fluid of patients taking aspirin at the time of knee arthroplasty surgery.
Abstract Low doses of acetylsalicylic acid (aspirin) are recommended in some venous thromboembolism prophylaxis guidelines for total knee replacement arthroplasty surgery. As salicylic acid has antimicrobial activity it would be advantageous if aspirin's use reduced the risk of prosthetic joint infection. We measured salicylate levels in the synovial fluid of patients taking long term low dose aspirin who were having knee arthroplasty surgery. Low levels of salicylic and salicyluric acids were present in synovial fluid immediately pre surgery. Acetylsalicylic acid was not measurable in synovial fluid. The measured...
Source: Clinical and Experimental Pharmacology and Physiology - May 5, 2020 Category: Drugs & Pharmacology Authors: Eisen DP, McEwen P, Hansen R, Pandey S, Wallis SC, Roberts JA Tags: Clin Exp Pharmacol Physiol Source Type: research

Investigating the role of endogenous opioid system in chloroquine-induced phospholipidosis in rat liver by morphological, biochemical and molecular modeling studies.
Abstract Drug-induced phospholipidosis (DIPL) is characterized by phospholipid storage in the lysosomes of affected tissues. Many severe effects and toxicities have been linked to DIPL. The aim of this study was to determine whether the endogenous opioid system is involved in chloroquine-induced phospholipidosis. The effect of naltrexone as an antagonist of opioid receptors in chloroquine-induced phospholipidosis in rat liver was investigated by morphological, biochemical, and molecular modeling studies. Transmission electron microscopy (TEM) showed that morphological characteristic changes of rat liver, including...
Source: Clinical and Experimental Pharmacology and Physiology - May 4, 2020 Category: Drugs & Pharmacology Authors: Malek MR, Ahmadian S, Dehpour AR, Ebrahim-Habibi A, Shafiezadeh M, Kashani Amin E Tags: Clin Exp Pharmacol Physiol Source Type: research

Hyperbaric oxygen treatment mitigates liver damage in mice with noise exposure.
Abstract Noise exposure relates to various pathological disorders including liver damage, preventive measures of which are being demanded. Hyperbaric oxygen treatment (HBOT), as a non-invasive procedure, exerts convincing therapeutic potency on multiple liver diseases. The efficacy of HBOT in mitigating noise induced liver damage (NILD) and associated mechanisms would be elucidated here. Mice were subject to broad band noise (20-20k Hz, 90-110 dB) for 5 days by 3 hours/day. HBOT with 2.5 atmosphere absolute (ata) was employed before noise exposure. Morphology of liver tissue was examined by hematoxylin-eosin (HE) ...
Source: Clinical and Experimental Pharmacology and Physiology - April 29, 2020 Category: Drugs & Pharmacology Authors: Meng X, Zhang X, Su Y, Gu Z, Xie X, Chang Y, Bao J Tags: Clin Exp Pharmacol Physiol Source Type: research

The association between serum albumin/prealbumin level and disease severity in non-CF bronchiectasis.
In conclusion, BMI, albumin and prealbumin showed a significant correlation with the BSI, FACED, as well as patients' clinical symptoms. Among them, serum albumin was the indicator most strongly associated with the BSI and questionnaires, while prealbumin could better reflect lung function decline and radiological severity. PMID: 32347970 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - April 29, 2020 Category: Drugs & Pharmacology Authors: Li L, Li Z, Bi J, Li H, Wang S, Shao C, Song Y Tags: Clin Exp Pharmacol Physiol Source Type: research

Comparison of effects of L/N-type and L-type calcium channel blockers on post-infarct cardiac remodeling in spontaneously hypertensive rats.
Abstract Hypertension and coronary events are becoming more prevalent in aging societies, and myocardial infarction usually occurs in calcium channel blocker (CCB)-treated hypertensive patients. We herein compared the effects of cilnidipine, an L/N-type CCB and amlodipine, an L-type CCB, on post-infarct left ventricular (LV) remodeling in spontaneously hypertensive rats (SHRs). Male SHRs were subjected to 30 minutes of left coronary artery occlusion followed by reperfusion (MI group). The administration of cilnidipine (10 mg/kg/day; MI+Cil group) or amlodipine (10 mg/kg/day; MI+Aml group) was initiated one week be...
Source: Clinical and Experimental Pharmacology and Physiology - April 23, 2020 Category: Drugs & Pharmacology Authors: Naseratun N, Kobara M, Watanabe Y, Toba H, Nakata T Tags: Clin Exp Pharmacol Physiol Source Type: research

1,25-Dihydroxyvitamin D3 Prevents Deleterious Effects of Erythromycin on Mitochondrial Function in Rat Heart Isolated Mitochondria.
In this study, we showed that erythromycin impairs mitochondrial function and induces mitochondrial toxicity in rat heart isolated mitochondria, which were reverted by calcitriol. These findings suggest that 1,25-dihydroxivitamin D3 may be a preventive/therapeutic strategy for cardiotoxicity complications caused by erythromycin. PMID: 32314438 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - April 20, 2020 Category: Drugs & Pharmacology Authors: Mersa A, Atashbar S, Ahvar N, Salimi A Tags: Clin Exp Pharmacol Physiol Source Type: research

Analysis of Very Important Pharmacogenevariants in the Tibetan population from China.
Abstract Personalized medicine, the best treatment suited for an individual, is a hot field of clinical research in the world. Many recent studies have shown that genetic variations had a great influence on the treatment. The study aimed to identify the distribution differences of very important pharmacogene (VIP) variants between the Tibetan population and the 26 populations. Based on the PharmGKB database, we successfully genotyped 50 VIP variants located in 27 genes in the Tibetan population. We also compared the genotype frequencies of VIP variants between Tibetan and the 26 populations from the 1000 Genomes p...
Source: Clinical and Experimental Pharmacology and Physiology - April 20, 2020 Category: Drugs & Pharmacology Authors: Rong H, Dong H, He X, Yuan D, Bai M, Wang L, Liu T, He Y, Zheng J, Wang Y, Jin T Tags: Clin Exp Pharmacol Physiol Source Type: research

Evidence for Altered Insulin Signaling in the Brains of Genetic Absence Epilepsy Rats from Strasbourg.
Abstract Insulin-mediated signaling in the brain is critical for neuronal functioning. Insulin resistance is implicated in the development of some neurological diseases, although changes associated with absence epilepsy have not been established yet. Therefore, we examined the major components of PI3K/Akt-mediated insulin signaling in cortical, thalamic, and hippocampal tissues collected from Genetic Absence Epilepsy Rats from Strasbourg (GAERS) and Non-Epileptic Control (NEC) rats. Insulin levels were also measured in plasma and cerebrospinal fluid (CSF). For the brain samples, the nuclear fraction (NF) and total...
Source: Clinical and Experimental Pharmacology and Physiology - April 18, 2020 Category: Drugs & Pharmacology Authors: Sekar S, Marks WN, Gopalakrishnan V, Greba Q, Snutch TP, Howland JG, Taghibiglou C Tags: Clin Exp Pharmacol Physiol Source Type: research

Long non-coding RNA DDX11-AS1 promotes non-small cell lung cancer development via regulating PI3K/AKT signaling.
Abstract Non-small cell lung cancer (NSCLC) has been considered to be the most common category of lung cancer, occupying approximately 80% of lung cancers. Long non-coding RNAs (lncRNAs) were diffusely documented to modulate carcinogenesis or progression of tumors. However, the role of DDX11-AS1 was still unclear in NSCLC. Bioinformatics analysis and experimental assays including hematoxylin and eosin (H&E) staining, RT-qPCR, colony formation, CCK-8, flow cytometry, Western blot and xenograft assays were applied to investigate the biological role and molecular mechanism of DDX11-AS1 in NSCLC. The level of lncR...
Source: Clinical and Experimental Pharmacology and Physiology - April 16, 2020 Category: Drugs & Pharmacology Authors: Feng X, Yang S, Zhou S, Deng S, Xie Y Tags: Clin Exp Pharmacol Physiol Source Type: research

A Glimpse at Growth Hormone Releasing Hormone Cosmos.
Abstract Growth hormone releasing hormone is a hypothalamic neuropeptide, which regulates the secretion of growth hormone by the anterior pituitary gland. Recent evidence suggest that it exerts growth factor activities in a diverse variety of in vivo and in vitro experimental malignancies, which are counteracted by growth hormone releasing hormone antagonists. Those peptides support the lung endothelial barrier integrity by suppressing major inflammatory pathways, and by inducing the endothelial defender p53. The present effort provides information regarding the effects of growth hormone releasing hormone in the r...
Source: Clinical and Experimental Pharmacology and Physiology - April 14, 2020 Category: Drugs & Pharmacology Authors: Barabutis N Tags: Clin Exp Pharmacol Physiol Source Type: research

Rotenone restrains the proliferation, motility and epithelial-mesenchymal transition of colon cancer cells and the tumorigenesis in nude mice via PI3K/AKT pathway.
This study aims to clarify the effect of rotenone on the proliferation, apoptosis, invasion and migration of colon cancer cells and tumorigenesis in nude mice. The present results showed that rotenone significantly inhibited the proliferation, promoted the apoptosis, and suppressed the invasion and migration of colon cancer cells in a dose-dependent manner. Rotenone inhibited PI3K/AKT pathway in LoVo and SW480 cells in a dose-dependent manner. In addition, rotenone regulated the proliferation, apoptosis, invasion, migration and EMT of LoVo and SW480 cells through PI3K/AKT pathway. In colon cancer xenograft mice, rotenone i...
Source: Clinical and Experimental Pharmacology and Physiology - April 13, 2020 Category: Drugs & Pharmacology Authors: Xue W, Men S, Liu R Tags: Clin Exp Pharmacol Physiol Source Type: research

Up-regulation of miR-135b expression induced by oxidative stress promotes the apoptosis of renal tubular epithelial cells under high glucose condition.
In conclusion, these results indicated that the up-regulation of miR-135b expression induced by oxidative stress promotes the apoptosis of HK-2 cells under high glucose conditions. PMID: 32278326 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - April 11, 2020 Category: Drugs & Pharmacology Authors: Xiao L, Luo D, Pi P, Hu ML, Li XY, Yin QQ Tags: Clin Exp Pharmacol Physiol Source Type: research

Ginsenoside 20(S)-Rg3 upregulates HIF-1 α-targeting miR-519a-5p to inhibit the Warburg effect in ovarian cancer cells.
In conclusion, 20(S)-Rg3 upregulates miR-519a-5p via reducing DNMT3A-mediated DNA methylation to inhibit HIF-1α-stimulated Warburg effect in ovarian cancer. PMID: 32271958 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - April 9, 2020 Category: Drugs & Pharmacology Authors: Lu J, Chen H, He F, You Y, Feng Z, Chen W, Li X, Zhao L Tags: Clin Exp Pharmacol Physiol Source Type: research

Atorvastatin attenuates vascular remodeling in spontaneously hypertensive rats via the protein kinase D/extracellular signal-regulated kinase 5 pathway.
Abstract The present study was conducted to determine whether atorvastatin reduces hypertension-induced vascular remodeling and whether its effects involve protein kinase D (PKD) and extracellular signal-regulated kinase 5 (ERK5). We used 16-week-old spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto(WKY) rats. The blood pressure and serum lipid concentration were measured. Changes in the vascular morphology and histology were examined using H&E, Masson, s tricrome, and Sirius Red staining. The media thickness (MT), ratio of MT to lumen diameter (LD) (MT/LD), collagen volume fraction (CVF) and...
Source: Clinical and Experimental Pharmacology and Physiology - April 7, 2020 Category: Drugs & Pharmacology Authors: Yuan H, Wang D, Zhang Y, Geng J Tags: Clin Exp Pharmacol Physiol Source Type: research

MiR-342 attenuates lipopolysaccharide-induced acute lung injury via inhibiting MAPK1 expression.
This study will explore the role of miR-342/MAPK1 axis in regulation of lipopolysaccharide (LPS)-induced ALI. We found miR-342 was downregulated in LPS-induced A549 cells compared with control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Overexpression of miR-342 reduced LPS-induced inflammatory responses and apoptosis in LPS-stimulated A549 cells, and had a protective role in LPS-treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual-luciferase reporter gene assay demonstrated that miR-342 reg...
Source: Clinical and Experimental Pharmacology and Physiology - April 5, 2020 Category: Drugs & Pharmacology Authors: Zhu S, Song W, Sun Y, Zhou Y, Kong F Tags: Clin Exp Pharmacol Physiol Source Type: research

MiR-599 regulates LPS-mediated apoptosis and inflammatory responses through the JAK2/STAT3 signaling pathway via targeting ROCK1 in human umbilical vein endothelial cells.
Abstract MicroRNA plays an integral role in the development of atherosclerosis. Our study aimed to investigate the roles of miR-599 in LPS-induced endothelial damage in human umbilical vein endothelial cells (HUVECs). HUVECs were transfected with a miR-599 mimic and negative control, and then exposed to LPS. The expression of miR-599 was detected by Quantitative real time-polymerase chain reaction (RT-qPCR). Cell viability was analyzed by CCK-8 assay and Trypan blue exclusion assay; the formation of DNA fragments was tested by Cell Death Detection Elisa Plus kit; the incidence of apoptosis was detected by flow cyt...
Source: Clinical and Experimental Pharmacology and Physiology - April 5, 2020 Category: Drugs & Pharmacology Authors: Wang J, Du A, Wang H, Li Y Tags: Clin Exp Pharmacol Physiol Source Type: research

Characterization of B cell-mediated PD-1/PD-L1 interaction in pancreatic cancer patients.
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a common type of pancreatic cancer with one of the worst survival rate of all malignancies. Recent studies have identified that immunosuppressive B cells could employ the PD-1/PD-L1 pathway to suppress antitumor T cell responses, hence, we examined the expression and function of PD-L1 in B cells. We found that the PD-L1 expression was significantly enriched in tumor-infiltrating (TI) B cells than in peripheral blood (PB) B cells from the same patients. Additionally, the PB B cells from stage III and stage IV PDAC patients presented significantly higher PD-L1 than...
Source: Clinical and Experimental Pharmacology and Physiology - April 4, 2020 Category: Drugs & Pharmacology Authors: Tong DN, Guan J, Sun JH, Zhao CY, Chen SG, Zhang ZY, Zhou ZQ Tags: Clin Exp Pharmacol Physiol Source Type: research

Sex-biased ceRNA Networks Reveal that OSCAR Can Promote Proliferation and Migration of Lung Adenocarcinoma in Women.
In this study, we constructed gender-independent, male-specific, and female-specific ceRNA networks using RNA sequencing results from TCGA database. Subsequently, through analysis of the core genes of the ceRNA network, we determined that the male and female ceRNA networks indeed display different features. In addition, we also found that the osteoclast-associated receptor (OSCAR) gene was a potential diagnostic target for detecting LUAD in females, and that increased expression of this gene promoted the proliferation and migration of A549 and H1975 LUAD cell lines; more specifically, A549 and H1975 are male and female LUA...
Source: Clinical and Experimental Pharmacology and Physiology - April 4, 2020 Category: Drugs & Pharmacology Authors: Wang D, Yang N, Xie S Tags: Clin Exp Pharmacol Physiol Source Type: research

Determination of pharmacokinetic parameters of vitamin K1 in rats after an intravenous infusion.
Abstract Pharmacokinetic parameters of vitamin K1 have a large range of values in different literature. The aim of this study was to determine the pharmacokinetic parameters of vitamin K1 following post-constant speed intravenous infusion (PCSII) to provide rational pharmacokinetic parameters of vitamin K1 and compare these with results of noncompartmental analysis following intravenous injection (IV). After 15h intravenous infusion of vitamin K1 in rats, the logarithmic concentration-time curve of vitamin K1 was fit to a linear equation following PCSII (R2 =0.9599±0.0096). Then, half-time (T1/2 ), apparent...
Source: Clinical and Experimental Pharmacology and Physiology - March 29, 2020 Category: Drugs & Pharmacology Authors: Mi YN, Yan PP, Yu RH, Li QG, Wang CC, Hui MQ, Cao L, Cao YX Tags: Clin Exp Pharmacol Physiol Source Type: research

Tryptophan 387 and 390 residues in ADAMTS13 are crucial to the ability of vascular tube formation and cell migration of endothelial cells.
Abstract A disintegrin and metalloproteinase with thrombospondin motifs 13 (ADAMTS13) was mainly generated and secreted from endothelial cells (ECs). Our previous study showed that tryptophan (Trp) residues at 387 and 390 in ADAMTS13 are required for its secretion and enzymatic activity. However, the effects on its host cell as well as the potential mechanism have not been clear. The aim of the study was to examine the effects of Trp residues 387 and 390 of ADAMTS13 on the biological processes of ECs. Herein, Trp was substituted with alanine in ADAMTS13 to generate ADAMTS13 mutants at 387 (W387A), 390 (W390A), and...
Source: Clinical and Experimental Pharmacology and Physiology - March 29, 2020 Category: Drugs & Pharmacology Authors: Liu L, Ma Z, Zhou X, Yin J, Lu J, Su J, Shen F, Xie L, Hu S, Ling J Tags: Clin Exp Pharmacol Physiol Source Type: research

Diosmetin exerts cardioprotective effect on Myocardial Ischemia injury in neonatal rats by Decreasing Oxidative Stress and Myocardial Apoptosis.
Abstract Myocardial injury caused by the myocardial ischemia (MI) is still a troublesome condition in the clinic, including apoptosis, oxidative stress and inflammation. Diosmetin inhibits the cellular apoptosis and inflammatory response and enhances antioxidant activity. So this study was designed to investigate the cardioprotective effects of diosmetin on MI model neonatal rats. Forty sprague dawley (SD) rats 7 days old were randomly divided into five groups. Four groups of rats received diosmetin (50, 100, and 200 mg/kg) or vehicle (MI group) after ischemia. Another group received vehicle without ischemia to se...
Source: Clinical and Experimental Pharmacology and Physiology - March 27, 2020 Category: Drugs & Pharmacology Authors: Mo GL, He Y, Zhang XQ, Lei X, Luo Q Tags: Clin Exp Pharmacol Physiol Source Type: research

The JAK2 inhibitor AG490 regulates the Treg/Th17 balance and alleviates DSS-induced intestinal damage in IBD rats.
This study aimed to evaluate the effect of AG490 on the intestinal inflammatory process in an IBD rat model. A dextran sulfate sodium (DSS)-induced IBD rat model was established, and disease activity index (DAI) scores were calculated. The histopathological damage score was determined by haematoxylin-eosin (H&E) staining. Treg/Th17 cells in the spleen were detected by flow cytometry. The levels of IL-10, IL-6 and IL-17A were detected by enzyme-linked immunosorbent assay (ELISA). AG490 attenuated DSS-induced IBD injury by regulating the Treg/Th17 balance and related cytokine secretion to reduce the DAI and colonic tissu...
Source: Clinical and Experimental Pharmacology and Physiology - March 25, 2020 Category: Drugs & Pharmacology Authors: Guo J, Wang LY, Wu J, Xu LF, Sun M Tags: Clin Exp Pharmacol Physiol Source Type: research

Dexmedetomidine in the  prevention of postoperative delirium in elderly patients following non-cardiac surgery: a systematic review and meta-analysis.
Dexmedetomidine in the prevention of postoperative delirium in elderly patients following non-cardiac surgery: a systematic review and meta-analysis. Clin Exp Pharmacol Physiol. 2020 Mar 25;: Authors: Shen QH, Li HF, Zhou XY, Yuan XZ Abstract The efficacy of dexmedetomidine in the prevention of postoperative delirium (POD) remains ambiguous, however, it has been used to reduce the incidence of delirium in elderly patients. Here, we conducted a meta-analysis study for assessing the effects of dexmedetomidine on POD among elderly patients following non-cardiac surgery. A systematic l...
Source: Clinical and Experimental Pharmacology and Physiology - March 25, 2020 Category: Drugs & Pharmacology Authors: Shen QH, Li HF, Zhou XY, Yuan XZ Tags: Clin Exp Pharmacol Physiol Source Type: research

Oncogenic pseudogene DUXAP10 knockdown suppresses proliferation and invasion and induces apoptosis of papillary thyroid carcinoma cells by inhibition of Akt/mTOR pathway.
In conclusion, DUXAP10 knockdown suppressed proliferation and invasion and induced apoptosis in PTC cells at least partially by inhibition of the Akt/mTOR pathway. PMID: 32215944 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Pharmacology and Physiology)
Source: Clinical and Experimental Pharmacology and Physiology - March 25, 2020 Category: Drugs & Pharmacology Authors: Li J, Jiang L, Liu Z, Li Y, Xu Y, Liu H Tags: Clin Exp Pharmacol Physiol Source Type: research