How far we have gone in realizing true potential of Viscum album as versatile regulator of cell signaling pathways.
Authors: Farooqi AA, Sabitaliyevich UY PMID: 31304899 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of the effects of Cyclotrichium niveum on brain acetylcholinesterase activity and oxidative stress in male rats orally exposed to lead acetate.
Authors: Sahin Z, Ozkaya A, Uckun M, Yologlu E, Kuzu M, Comakli V, Demirdag R, Tel AZ, Aymelek F, Yologlu S Abstract Cyclotrichium niveum is an endemic plant for Turkey and it appears to have in vitro antioxidant and acetylcholinesterase inhibition properties. To the best of our knowledge, there has been no study on the in vivo effects of this plant. Therefore, the purpose of this study was to evaluate the effects of C. niveum on lead (Pb)-acetate-induced potential alterations in brain acetylcholinesterase activity, as well as oxidative stress in male rats. The rats were randomly assigned to control, Pb-acetate, C....
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Impact of citrate- and chitosan-capped gold nanoparticles on the liver of Swiss albino mice: Histological and cyto-genotoxic study.
Authors: Zaki EI, El-Seedy AS, Kelada IP, Sharafeldin NA, Abdel Mouaty HM, Ramadan HS Abstract The present study aimed to disclose the histological alterations and cyto-genotoxic potential induced by citrate- and chitosan-capped AuNPs on liver of adult Swiss albino mice. Animals were randomly divided into 8 groups. The first two groups were intraperitoneally (i.p) injected with physiological saline once and left for 10 days and every other day for 21 days, respectively, and kept as negative control groups. While the third and fourth groups were injected i.p with a single dose of 2 mg/kg of citrate- and chitosan-cap...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective and therapeutic effects of Pilose antler against kidney deficiency-induced osteoporosis.
This study was carried out to evaluate the preventive and curative effects of Pilose antler against osteoporosis due to kidney deficiency, and investigate its potential mechanism of action. A model of osteoporosis due to kidney deficiency was established in rats using bilateral ovariectomy. Pilose antler polypeptide (PAP), Pilose antler polysaccharide (PAP'), and their mixture (PAP+PAP') were separately administered to the rats for 12 weeks, with progynova and xianlingubao tablets (XLGB) as the positive control groups. We determined the bone mineral density (BMD) and uterus Index of the rats. Osteoblastic bone metabolism-r...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Emodin attenuates Alzheimer's disease by activating the protein kinase C signaling pathway.
Authors: Du C, Shi L, Wang M, Mao P, Wang J, Wei Y, Hou J, Wang M Abstract To investigate the effects of emodin on learning and memory and protein kinase C (PKC) signaling pathway in Alzheimer's disease (AD) model mice. 60 APP/PS1 double transgenic AD mice were selected as model mice at the age of 7-8 months, 36 healthy male C57BL/6 mice served as the control group. Morris water maze method and passive avoidance experiment were used to evaluate the memory ability of mice. The thiazole blue (MTT) method and the lactate dehydrogenase (LDH) cytotoxicity test kit were used to evaluate the effect of emodin on the cell v...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Naringin inhibits lipopolysaccharide-induced activation of microglia cells.
Authors: Bai J, Li S, Wu G, Ma L, Li C, Liu H, Zhang P Abstract The purpose of this study was to investigate the effect of naringin on lipopolysaccharide (LPS)-induced activation of BV2 microglia and inflammatory factor release, and the mechanism involved. Different concentrations of naringin were used to pretreat BV2 cells for 30 min, after which they were stimulated with 100 ng/mL LPS for different durations. The levels of NO, IL-1β and TNF-α in the cell culture medium was determined with ELISA and Griess method. The mRNA expressions of IL-1β and TNF-α was determined with RT-PCR. Changes in ...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Interleukin-6 serum level and gene polymorphism in keloid patients.
In conclusion, there was a great association between IL-6 572 gene polymorphism and its serum level in patients with keloid specifically who have family history. PMID: 31304905 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The correlation between infections by human papillomavirus types 6/11 and 16/18 and mammary gland hyperplasia with glandular thickening.
Authors: Xiaoqun D, Xiaoli L, Jun L, Yang X Abstract The aim of the present study was to investigate the correlation between human papillomavirus (HPV) type 6/11 and 16/18 infections and glandular thickening mammary gland hyperplasia in order to explore methods for preventing glandular thickening mammary gland hyperplasia. A total of 240 patients with glandular thickening mammary gland hyperplasia who were treated by surgery in our hospital from January 2012 to June 2017 were enrolled in the present study. The hyperplastic breast tissue and adjacent normal breast tissue were taken to test HPV type 6/11 and 16/18 in...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of sakuranetin in brain cells of dementia model rats.
In this study, the effect of SAK on spatial discrimination in a rat model of cognitive dysfunction exposed to D-galactose was investigated with respect to its effect on malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels, and on the expressions of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and nuclear factor-κB inhibitory factor-α (IκBα) in hippocampus of rats. The results obtained suggest that SAK may exert protective effects on brain cells through anti-oxidation mechanism. Moreover, the improvement in learning and memory impairment by SA...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-epileptic effect of 16-O-acetyldigitoxigenin via suppressing mTOR signaling pathway.
Authors: Tu W, Qian S Abstract Epilepsy is a common chronic disease of the central nervous system that can last for years or even decades, causing serious adverse effects on the body, mind, and psychology of patients. Traditional antiepileptic drugs can effectively control seizures, but because of large individual differences, serious adverse reactions, narrow therapeutic window and other shortcomings, more effective, new treatment drugs are looked for. Streptocaulon griffithii is a plant of Asclepiadaceae. 16-O-acetyldigitoxigenin (ACE) is a strong cardiac glycoside isolated from methanol extract of Streptocaulon ...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of urea, compost, manure and bio-fertilizers on yield, percentage and composition of fatty acids of sesame seed oil (Sesamum indicum L.).
This study was carried out in Iran during two growing seasons 2016-2017. Sesame is one of the earliest oilseed plants, high oil percentage and high content of unsaturated fatty acids, especially oleic acid and linoleic acid, have led to an increase in the nutritional quality of sesame oil. Accordingly, a two-year experiment was conducted as factorial based on randomized complete block design with three replications. The evaluated factors included four levels of urea as nitrogen fertilizer (0, 33, 66 and 100% of plant requirement), three kinds of organic fertilizer (manure, vermicompost and control) and four kinds of biofer...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of Heweijiangni-decoction on esophageal morphology in a rat model of OVA-induced visceral hypersensitivity followed by acid exposure.
In this study, we observed the effect of HWJND on esophageal morphology in a rat model of ovalbumin (OVA)-induced visceral hypersensitivity followed by acid exposure. Esophageal morphology was assessed by measuring the extent of dilated intercellular spaces (DIS), desmosome disruption, and mitochondrial fragmentation. HWJND in low, moderate, and high doses relieved DIS and desmosome disruption in esophageal epithelium compared with model group (P<0.05 for all doses). In addition, HWJND in high dose protected mitochondria from fragmentation (P<0.05). Other findings suggest that DIS and mitochondrial fragmentat...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Camellia sinensis, Hypericum perforatum and Urtica dioica on kidney and liver injury induced by carbon tetrachloride in rats.
This study is aimed to investigate the effects of Camellia sinensis (CS), Hypericum perforatum (HP) and Urtica dioica (UD) in kidney and liver injury induced by carbon tetrachloride (CCl4) in rats. Highly toxic CCl4 which is used as a solvent in industry comprises experimental toxicity in rats and is widely used in hepatotoxicity and other tissue injury models. The purpose of this investigation is to monitor blood and various tissues by biochemical and histopathological analysis for preventive effects of CS, HP and UD on oxidative stress induced by administration of CCl4 and to enlighten the probable mechanism. Fifty eight...
Source: Cellular and Molecular Biology - July 17, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Delayed development influences the outcome of different grades of D5 and D6 blastocysts during freeze-thaw cycle.
This study included transfers from the Reproductive Medicine Center of the Second Affiliated Hospital of Wenzhou Medical University during freeze-thaw cycles from January 2016 to December 2017. They were divided into D5 group (1616 cases) and D6 group (619 cases) according to blastocyst formation and development. Each group was further divided into 5 groups according to the quality of the blastocyst and the number of transplants, making a total of 10 groups. Following the frozen transplantation cycle, the transplanting rate was significantly higher for D5 (41.73%) than for D6 (23.98%) (P < 0.05); the ongoing pregnan...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of SRAP marker efficiency in identifying the relationship between genetic diversities of corn inbred lines with seed quantity and quality in derived hybrids.
Authors: Fareghi S, Mirlohi AF, Saeidi G, Khamisabadi H Abstract In order to estimate the efficiency of SRAP markers for identifying the performance of seed quantity and quality in maize single crosses, 13 inbred lines obtained from CIMMYT germplasm bank were crossed to A679, K166B, K18 and MO17 testers using the line×tester method. The inbred lines and derived hybrids were evaluated in two experiments separately in a randomized complete block design with three replications during two growing seasons in 2014 and 2015. In order to evaluate genetic variation in the inbred lines, 25 SRAP markers were also used. ...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular detection of ALS1, ALS3, HWP1 and SAP4 genes in Candida Genus isolated from hospitalized patients in Intensive Care Unit, Tehran, Iran.
Authors: Ardehali SH, Azimi T, Fallah F, Aghamohammadi N, Alimehr S, Karimi AM, Azimi L Abstract Candida species are considered as one of the important cause of nosocomial and community infections. Candidacies are fourth caused by septicemia in some countries and possess extra cost to the health care system. The aim of this study was survey the presence of virulence factors associated with various candida geniuses in samples which have been collected from the intensive care unit. In this cross-sectional study, various clinical specimens have been collected from patients which hospitalized in the Intensive Care Unit...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clinical significance of HDAC9 in hepatocellular carcinoma.
Authors: Hu Y, Sun L, Tao S, Dai M, Wang Y, Li Y, Wu J Abstract In recent years, most related studies have found that chronic hepatitis B virus infection is the main cause of hepatocellular carcinoma (HCC), but the specific pathogenesis is still unclear. To investigate the function of HDAC in hepatocellular carcinoma (HCC), this study used qRT-PCR to determine the expression levels of miR-376a and HDAC9 mNRA in HCC and para-cancerous tissues. The clinical significance of HDAC9 in HCC was assessed in a study cohort containing 37 patients with HCC using immunohistochemistry. The expression level of miR-376a in liver ...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Humic acid reduces the CuO and ZnO nanoparticles cellular toxicity in rapeseed (Brassica napus).
Authors: Yarmohammadi A, Khoramivafa M, Honarmand SJ Abstract Concerns about nanoparticles environmental pollution risk have been increased globally due to an increase in the production of nanoparticles in recent years and their use in diverse cases. The purpose of this experiment was to study the alleviation effect of humic acid on nanoparticles toxicity in greenhouse conditions. Thus two separate experiments were conducted at the rosette growing stages of rapeseed in a factorial experiment as a completely randomized design with three replications. The first factor was copper and zinc oxide nanoparticle in five co...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-inflammatory, anti-nociceptive and sedative-hypnotic activities of lucidone D extracted from Ganoderma lucidum.
In conclusion, these results suggest the anti-inflammatory, analgesic and sedative effects of LUC in vitro and in vivo. PMID: 31078150 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A  hesperetin derivative plays a role in immunoregulatory effect on human macrophages.
This study provides a theoretical and practical basis for the development of HES as an immunomodulator in the future. PMID: 31078151 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ginsenoside compound K inhibits growth of lung cancer cells via HIF-1 α-mediated glucose metabolism.
Ginsenoside compound K inhibits growth of lung cancer cells via HIF-1α-mediated glucose metabolism. Cell Mol Biol (Noisy-le-grand). 2019 Apr 30;65(4):48-52 Authors: Chen HF, Wu LX, Li XF, Zhu YC, Wang WX, Xu CW, Huang ZZ, Du KQ Abstract Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related deaths. Compound K, an active metabolite of ginsenosides, is reported to exhibit anti-cancer property in various types of human malignancies. The present study investigated the role of compound K on glucose metabolism in NSCLC cells and its underlying mechanism. Our study found that compound...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of Hordeum vulgare on the monoaminergic system modulating neural-thyroid dysfunction in hypothyroid female rats.
Authors: Wahman LF, Abd-Rabo MM, Youseef MHM, Abdel-Hameed UK Abstract Thyroid hormones regulate the development and maturation of the brain by maintaining levels of neurotransmitters and their related metabolites. The present work emphasizes the neural dysfunction in the brain caused by hypothyroidism and the potential role of Hordeum vulgare (water soluble barley, (B)) in ameliorating these effects. The study was conducted on euothyroid and hypothyroid adult female rats. The induction of hypothyroidism was conducted by oral-administration of neo-mercazole (5.0 mg.kg-1) daily for thirty days prior the study and te...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Roles of Sema3A and VEGF165 in cortical neurons and vascular endothelial cells during oxygen glucose deprivation stimulation.
Authors: Yang Y, Li C, Wang R, Wang H, Ma Q, He Y, Zhang P Abstract To investigate the expressions and roles of semaphorin3A (Sema3A) and vascular endothelial growth factor 165 (VEGF165) in cultured rat cortical neurons and vascular endothelial cells after oxygen glucose deprivation (OGD) stimulation. Cultured cortical neurons (NC) and vascular endothelial cells (VEC) of Sprague Dawley (SD) rats (SPF grade) were randomly divided into control group and OGD treatment group. Western blot assay, immunofluorescent staining and immunohistochemical methods were used to determine the expressions of VEGF165, Sema3A and neur...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Palmatine plays a role in sedation and hypnosis by increasing 5-hydroxytryptamine.
Authors: Du J, Yu Y, Qiu L, Qiu M, Zhu Q Abstract Objective of this study was to investigate the sedative and hypnotic effects of palmatine and to observe whether its mechanism is related to 5-hydroxytryptamine (5-HT) and GABA. The sedative and hypnotic effects of palmatine on mice were observed with mouse autonomic activity test, direct sleep test, pentobarbital sodium in suprathreshold and subthreshold dose sleep test. The content of GABA and 5-HT in brain homogenate was determined by ELISA  method. Mouse brain specimens were observed by immunohistochemistry for 5-HT expression in the nucleus of mouse brain....
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ginkgetin inhibits proliferation of HeLa cells via activation of p38/NF- κB pathway.
Ginkgetin inhibits proliferation of HeLa cells via activation of p38/NF-κB pathway. Cell Mol Biol (Noisy-le-grand). 2019 Apr 30;65(4):79-82 Authors: Cheng J, Li Y, Kong J Abstract Effect of ginkgetin on proliferation of human cervical cancer (HeLa) cells and the underlying mechanism   were investigated. Human cervical cancer (HeLa) cells were cultured at 37 °C in 10 % fetal bovine serum (FBS) supplemented RPMI 1640 medium in a humidified incubator containing 5 % CO2. Cell proliferation was determined using MTT assay, while real-time quantitative polymerase chain reaction (qRT-PCR) a...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hydro-thermal priming enhance seed germination capacity and seedling growth in sugar beet.
In this study, the influence of hydrothermal priming on seed parameters of sugar beet is investigated in both laboratory and field conditions. In the laboratory, the treatments consist of a combination of cultivars (Arya and Shokoofa), hydro-priming at two temperatures (10 and 15 °C) for 6, 10, 14, 18, 22 hours. Germination traits and seedling growth were measured for determination of optimum hydro-thermal priming. Also, the protein pattern in the optimum hydro-thermal priming treatments and unprimed seeds were compared by electrophoresis. In the field experiment, the percentage and rate of emergence of primed and unpr...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of combination of all-trans retinoic acid and arsenic trioxide on apoptosis of acute promyelocytic leukemia cells.
Authors: Hu J, Sun Q, Fang W, Wang Q Abstract To study the effect of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) combination treatment on apoptosis of acute promyelocytic leukemia cells (NB4), inflammation and prognosis. The effect of ATRA - ATO combination on the proliferation of NB4 was determined using MTT assay. Apoptosis of NB4 cells was assessed with TUNEL assay. The effect of ATRA-As2O3 combination on the expressions of IL-6 and TNF-α in NB4 cells was determined using ELISA kits, while its effect on the quality of life of 25 acute promyelocytic leukemia patients admitted to our hospital w...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lipopolysaccharide pretreatment inhibits oxidative stress-induced endothelial progenitor cell apoptosis via a TLR4-mediated PI3K/Akt/ NF- κB p65 signaling pathway.
In this study, the role of LPS in apoptosis of EPCs was investigated. Pretreatment with 1µg/ml LPS prevented oxidative stress-induced EPCs apoptosis and ROS generation, which effects were abolished by TAK-242, a specific TLR4 antagonist. Further investigation of the mechanisms demonstrated that the activation was mediated by TLR4, and that PI3K/Akt/ NF-κB p65 signaling pathway may play a critical role in the process. PMID: 31078159 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nppc/Npr2/cGMP signaling cascade maintains oocyte developmental capacity.
Authors: Celik O, Celik N, Ugur K, Hatirnaz S, Celik S, Muderris II, Yavuzkir S, Sahin İ, Yardim M, Aydin S Abstract The follicle must fulfill the following criteria if it is to survive the period between early embryonic life and the luteinizing hormone (LH) peak. It should (i) be surrounded by pregranulosa cells; (ii) complete the first meiotic division and become dormant; and (iii) continue metabolism during the dormant stage. Interaction between the natriuretic peptide precursor type C (Nppc) and its receptor, natriuretic peptide receptor 2 (Npr2), affects female fertility through the production of oocytes with...
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ABC, a patented innovation in the infusion of teas and herbal plants: enrichment of Camellia sinensis leaves with dry extracts from herbal plants.
In conclusion, the ABC process offers a real breakthrough in the market of teas and herbal teas for health and wellness. PMID: 31078161 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 14, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Retraction: 6-Gingerol inhibits proliferation in gastric cancer via the STAT3 pathway in vitro.
Authors: Cellular and Molecular Biology Editor Abstract [This delete the article DOI: 10.14715/cmb/2019.65.3.16]. PMID: 30963995 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 11, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of gibberellic acid and zinc sulfate on biochemical performance relate to drought tolerance of white bean under water stress.
In this study the effects of zinc sulfate and gibberellin on agro physiological of white bean under water deficiency were studied. Therefore, an experiment was conducted in a split-split plot design based on a randomized complete block with three replications in two places. The experimental factors included three irrigation levels, spraying of zinc sulfate in four levels and two levels of non-spraying and spraying of gibberellin. Analysis of measured data indicated that the water stress had a significant effect on all traits, except proline amount and 100 seeds weight. Spraying of zinc sulfate showed a significant effect o...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.).
Authors: Dastmalchi T, Omidi M, Azizinezhad R, Rezazadeh S, Etminan A Abstract The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on thebaine and sanguinarine productions in vitro were investigated. Roots as explant and supplementing 3 mg L-1 2,4-Dichlorophenoxyacetic acid with 0.5 mg L-1 Benzyl amino purine to modified MS medium were selected to achieve the most efficient...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Impact of priming on seed germination, seedling growth and gene expression in common vetch under salinity stress.
This study was conducted to elucidate the effects of osmopriming with polyethylene glycol 6000 (PEG-6000) on seed germination, seedling growth and gene expression in the common vetch (Vicia sativa L.) in different saline conditions. Common vetch seeds were primed with PEG-6000 solutions having different osmotic potentials (0.00, -0.50,  -0.75, -1.00, -1.25, and -1.50 MPa) for 12 hours. Control (un-primed) and primed seeds were germinated and seedlings were grown in different saline conditions (EC= zero, 4, 8 and 16 dS m-1). Furthermore, gene expression was compared in the primed seedlings in two different osmotic pote...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Gene cloning, expression pattern analysis, and subcellular localization of LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) homologs in chrysanthemum (Chrysanthemum morifolium Ramat.).
Authors: Wu Z, Gao Y, Fan M, Gao Y Abstract Flowering is a very important developmental stage in the plant life cycle. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) has been shown to participate in epigenetic silencing of flowering genes. Here, for the first time, we isolated and characterized six CmLHP1 homolog genes from the important day-neutral ornamental Chrysanthemum morifolium cultivar 'Jin budiao'. These homolog genes were most likely generated by whole-genome duplication. Bioinformatic analysis showed that chrysanthemum LHP1 homologs present low similarity to other plant LHP1-like genes. However, three nuclear loc...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Indole itself and its novel derivative affect PML cells proliferation via controlling the expression of cell cycle genes.
Authors: Noroozi MK, Mahmoodi M, Jafarzadeh A, Darehkordi A, Hajizadeh MR, Khorramdelazad H, Sayadi AR, Falahati-Pour SK, Hassanshahi G Abstract Recently the role of indole and pyran rings in carcinogenesis has been well studied. Here we studied the effects and the possible mechanisms of the action of basal indole (I3A) and its novel indole derivative (C19H15F3N2O) on inhibition of proliferation cells in acute promyelocytic leukemia NB4 cell line by examining the expression of cell cycle genes. We treated NB4 cells with concentration of C19H15F3N2O for 24-72 h. The MTT and PI/Annexin V examinations were employed fo...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evolutionary analysis of TSP-1 gene in Plateau zokor (MyospalaxBaileyi) and its expression pattern under hypoxia.
In conclusion, due to long-term adaption to the hypoxic environment of sealed burrows, plateau zokor upregulates the expression of TSP-1 to effect anti-angiogenesis. Moreover, mutations in gene structure of TSP-1 may play an important role in inhibiting angiogenesis. PMID: 30942155 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Variations in the lipid peroxidation and antioxidant biomarkers in some tissues of anadromous cyprinid fish during migration.
This study reports that alterations occurred in the antioxidant defense system indicators in the tissues of anadromous cyprinid fish during migration and that the antioxidant defenses might reflect an important role in spawning migration that ultimately leads to accomplishment of reproductive activity. PMID: 30942156 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The concurrent effect of acyclovir and rosemary on glioblastoma cell culture.
In this study we aimed to investigate if ACV had cytotoxic effect on glioblastoma cell line U87 MG and also the effect of ACV on healthy cells. Furthermore it was aimed to search the effect of Rosmarinus Officinalis also known as rosemary which is an aromatic, perennial plant concurrent with ACV on glioblastoma and healthy cells. PMID: 30942157 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of antioxidant combination on indomethacin-induced gastric mucosal injury in rats.
Authors: Turkyilmaz IB, Coskun ZM, Bolkent S, Yanardag R Abstract The aim of this study is an investigation the protective effects of vitamin C (Vit C), vitamin E (Vit E), β-carotene, sodium selenate combination in indomethacin-induced gastric mucosal damage in rats. Rats were divided into 6 groups. Group I: Intact animals (control). Group II: Control animals receiving Vit C (100 mg/kg/day), Vit E (100 mg/kg/day), β-carotene (15 mg/kg/day) and sodium selenate (0.2 mg/kg/day) for 3 days. Group III: Animals receiving 25 mg/kg indomethacin. Group IV: Animals receiving Vit C, Vit E, β-carotene and sodium...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of a novel missense eya4 mutation causing autosomal dominant non ‑syndromic hearing loss in a chinese family.
Identification of a novel missense eya4 mutation causing autosomal dominant non‑syndromic hearing loss in a chinese family. Cell Mol Biol (Noisy-le-grand). 2019 Mar 31;65(3):84-88 Authors: Xiao SY, Qu J, Zhang Q, Ao T, Zhang J, Zhang RH Abstract The aim of this study was to identify the novel missense eya4 mutation which cause autosomal dominant non syndromic hearing loss In a Chinese family. Hearing loss is the most common sensory deficit in humans, but the middle-frequency sensorineural hearing loss (MFSNHL) is rare among hereditary non-syndromic hearing loss, and EYA4 is one of the genes reported ...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Influence of macerated fenugreek (Trigonella foenum graecum) oil added to trout feed at the different rates on the Feed Conversion Rate (FCR), body length, blood parameters and Nitroblue Tetrazolium (NBT) values ​​of rainbow trout (Oncorhynchus mykiss Wal.
In this study, 1% and 2% of macerated fenugreek oil was added to the feeds of rainbow trout with an average weight of 25.79 ± 1.5 g. At the end of the study, growth rate, blood parameters and NBT (Nitroblue Tetrazolium) level of rainbow trout were determined. The best feed ratio (FCR) was observed in the control group (0.77). Statistically significant differences were found only in MID values (P<0.05), although there was a numerical increase in all blood parameters. There was no statistically significant difference between NBT levels (P> 0.05). Although the best weight gain was in the control group as ...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between the methylation levels of Twist gene and pathogenesis of endometriosis.
Authors: Juanqing L, Hailan Y, Xiangwei F, Tao Z, Junyan M, Jianhong Z, Jun L, Jianhua Y Abstract The purpose of this study was to investigate the difference of Twist gene promoter methylation among ovarian ectopic endometrium, eutopic endometrium and non-endometriosis (EMs) endometrium . 15 patients with reproductive age hospitalized at Department of obstetrics and gynecology affiliated to Medicine School of Zhejiang University from January 2013 to June 2016 were selected. Among them, 5 patients underwent laparoscopic surgery due to ovarian type EMs, and were selected after histologic confirmation. Ectopic endomet...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of cytotoxic, genotoxic, and apoptotic effects of curcumin on glioma cells.
We examined concentration-dependent cytotoxic, genotoxic, apoptotic, and ROS generating effects of curcumin at C-6 cells and L-929 cells. The cells were incubated with different doses of curcumin (10-100 µM) for 24 hours. Higher doses of curcumin resulted in greater cellular death of cancer than of normal cells at higher concentrations. Curcumin also induced ROS generation in cancer than normal cells in a concentration-dependent manner. Our results showed that curcumin-induced DNA damage in a dose-dependent manner (p < 0.001). At high curcumin concentration such as 80 µM, the proportions of live cells in...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

6-Gingerol inhibits proliferation in gastric cancer via the STAT3 pathway in vitro.
In this study, we explored the role of 6-Gingerol (GI), a natural active ingredient, in treating gastric cancer cells. MTT assay and colony formation assay were utilized to confirmed that GI can control the proliferation of gastric cancer cells, which is time and concentration-dependent to some extent. The Annexin V-FITC/PI staining results by flow cytometry reveal that GI induces the apoptosis of gastric cancer cells. And a study on further pathways by western blot shows that GI brings about cell apoptosis by inhibiting the activation of STAT3. GI therefore may be a good candidate for treating gastric cancer. PMID: 30...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ent-Dihydrotucumanoic acid promotes apoptosis in PC-3 human prostate cancer cells.
Authors: Zhou M, Li W Abstract Prostate cancer (PC) has become a disease that pose a serious threat to men's health and life. In recent years, due to the changes of environment, lifestyle and other factors, the incidence of PC has been increasing rapidly in recent years, which is a serious threat to men's health. Ent-Dihydrotucumanoic Acid (DTA) is a compound isolated from Asteraceae of gymnosperms, which has many pharmacological effects. The effect of DTA on the growth of tumor cell line was studied by CCK-8 method, mitochondrial membrane potential and apoptosis were detected by flow cytometry, apoptosis-related g...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Piperazine ferulate exerts antihypertensive effect and improves endothelial function in vitro and in vivo via the activation of endothelial nitric oxide synthase.
Authors: Jianzhi S, Qizeng W, Bin L, Wenhui L, Yunpeng C, Chenrong F, Lin Z, Huiting C Abstract To investigate the effect of piperazine ferulate (PF) on hypertension and endothelial function, and to assess the possible underlying mechanism. Human umbilical vein endothelial cells (HUVEC), adult male Wistar Kyoto (WKY) rats aged 12 to 14 weeks, and spontaneously hypertensive (SH) and Sprague Dawley (SD) rats were used for this study. Cell viability, activities of angiotensin-converting enzyme (ACE) and heme oxygenase-1 (HO-1), in vivo NO synthesis, arterial systolic blood pressure, vascular function, expressions of e...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular role of peptides/proteins in subfertility of polycystic ovarian syndrome.
Authors: Celik O, Aydin S, Celik N, Ugur K, Yavuzkir S, Hatirnaz S, Yardim M, Celik S Abstract Obesity and hyperandrogenemia are known to have adverse effects on both developing follicle and endometrium receptivity in polycystic ovarian syndrome (PCOS). Insulin resistance also contributes to this dilemma as a cause or a consequence and leads to worsening of the clinical picture. The difficulty in obtaining pregnancy despite the presence of a large number of oocyte has concentrated our attention on oocyte quality and development. However, the occurence of subfertility has also caused us to investigate the presence o...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of Kisspeptin mediated signaling by non-coding RNAs in different cancers: the beginning of a new era.
Authors: Farooqi AA, Attar R, Bageshlooyafshar B, Sabitaliyevich UY, Nurmurzayevich SB, Yelekenova AB, Gormus U Abstract Kisspeptin-driven intracellular signaling has captured enormous attention because of its central role in cancer onset and progression. Wealth of information has helped us to develop a better understanding of the critical roles of Kisspeptin-mediated signaling in different cancers. However, astonishingly, we have not yet drilled down deep into the mysterious aspects associated with non-coding RNA mediated regulation of Kisspeptin-driven signaling. Therefore, in this mini-review, we will comprehens...
Source: Cellular and Molecular Biology - April 5, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mitochondrial gene cytochrome c oxidase I (CO1) used for molecular identification of Bactrocera zonata in Pakistan.
Authors: Amad I, Hafeez F, Khan MA, Nahid N, Javed MR, Shaheen S, Farooq M, Khan AZ, Hussain K Abstract Bactrocera zonata is fruit pest mostly attacked on peach and cause heavy destruction in production of peach fruits by sucking their juice. For their management, we start to detect them on basis of their molecular characterization. As mitochondrial genome encodes a gene COI used as biomarker for identification of eukaryotes including insects. In present study, we amplified COI gene and cloned into pTZ57R/T vector (Fermentas). Cloned gene was confirmed through restriction analysis and sequenced through its entirety...
Source: Cellular and Molecular Biology - March 19, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research