The relationship between gastric cancer and Helicobacter pylori cytotoxin-related gene A genotypes.
This study investigates the association between cagA H. pylori genotypes and gastric cancer. For this purpose, 65 stomach biopsies of the gastric cancer patients and 100 saliva samples were collected from healthy and H. pylori-infected individuals. Then genomic DNA was purified and Polymerase Chain Reaction (PCR) was performed for the studied gene using specific primers. The presence of H. pylori was investigated by PCR and a pair of specific primers for a protected region in the bacterium glmM gene. Then cagA+ and cagA- genotypes frequencies were determined in H. pylori-infected cases. Statistical analysis showed that the...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expressions of IL-12 and its receptors in patients with lumbar disc herniation and their relationship with clinical efficacy.
In conclusion, the concentrations of serum IL-12, IL-23R and IL12RB2 in LDH patients are significantly higher than those in normal controls. Moreover, the concentrations are closely related to the rehabilitation of patients and are expected to become therapeutic targets for LDH. PMID: 33287915 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of miR-7 on Hcy-induced rat cerebral arterial vascular smooth muscle cell proliferation, migration and inflammatory factor expression by targeting MMP-14 to regulate TLR4/NF- κB signaling pathway.
Effects of miR-7 on Hcy-induced rat cerebral arterial vascular smooth muscle cell proliferation, migration and inflammatory factor expression by targeting MMP-14 to regulate TLR4/NF-κB signaling pathway. Cell Mol Biol (Noisy-le-grand). 2020 Oct 31;66(7):12-17 Authors: Ma H, Wang L, Lv W, Lv Z Abstract The current research aimed to investigate the effect of miR-7 targeting matrix metalloproteinase 14 (MMP-14) on homocysteine (Hcy)-induced rat cerebral artery vascular smooth muscle cells (VSMCs) proliferation, migration and inflammatory factor expression and its possible mechanism. The expression o...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CircB3GNTL1 and miR-598 regulation effects on proliferation, apoptosis, and glutaminolysis in gastric cancer cells.
This study was performed to research circB3GNTL1 control, miR-598 and its mechanism for cell proliferation, apoptosis and glutamine breakdown. For this purpose, CirB3GNTL1 and miR-598 expressions were detected by qRT-PCR in gastric and cell lines; MTT tests were performed to detect proliferation; flow cytometry was established in flow; glutamine decomposition was evaluated with glutamine, glutamic acid and α-keto-glutaric acid (α-KG) expression; Bcl-2, PCNA, ASCT2 and GLS1 expression levels were calculated; Methods of expression were calculated. The results showed that CircB3GNTL1 expression was up-regulated an...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Multi-omics analysis profile oral tumor module clusters to reveal the potential pathogenic mechanism.
This study is based on a comprehensive strategy to explore a multi-factor-driven oral cancer barrier module, which is an attempt to describe the pathogenesis of the disease and potential regulatory drugs from a global perspective. Functional disease modules were identified by constructing a protein-specific interaction network in patients' oral tissues. Then, comprehensive pathogenesis was explored through combination with analysis of functional and signaling pathway enrichment, prediction of key regulatory factors. It was found that these specifically expressed proteins and their interactions often play a pivotal part&nbs...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Development of poly ADP-ribose polymerase-1 inhibitor with anti-cervical carcinoma activity.
Authors: Zhang TM, Wang W Abstract This research aimed to discover and identify new poly ADP-ribose polymerase-1 (PARP) inhibitors with potent anti-cervical carcinoma activity, and then explore their potential biological roles on cervical carcinoma cell. For this purpose, we identified a new PARP inhibitor from a high-throughput virtual screening method and found that the compound strongly inhibited cervical carcinoma HeLa cell. Cell proliferation was evaluated by an MTT assay, and the cell apoptosis was assessed by flow cytometry. Results showed that PARP1 is a poly ADP-ribose catalyzing enzyme in eukaryotic cells...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of sevoflurane on the spatial recall ability and expression of apolipoprotein E and β amyloid in the hippocampus in rats.
This study aimed to observe the recent spatial recall ability and the changes of expression of hippocampal apolipoprotein E (ApoE) and amyloid β protein (Aβ) in adult rats after inhaling sevoflurane anesthetic drugs, and to analyze the mechanism of action. For this purpose, a total of 54 adult SD clean-grade rats were selected in this study and were randomly divided into the sevoflurane anesthesia group, carrier gas group and control group, 18 rats in each group. The rats in the carrier gas group were inhaled with 1 L/min of oxygen O2+1 L/min air mixed carrier gas for 2 h, and the rats in the sevoflurane anesthes...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Circulatory miRNA-155, miRNA-21 target PTEN expression and activity as a factor in breast cancer development.
Authors: Ali SA, Abdulrahman ZFA, Faraidun HN Abstract Breast cancer is a complex disease with multiple factors involved in its pathophysiological development. genetic mutations of BRCA1, BRCA2 and p53 are among the most well-studied factors. The role of other genetic factors like altered expression profiles, SNPs in the regulatory regions of different genes or epigenetic factors like promoter methylation and histone modifications are also well studied but no solid understanding is available on distinct key players triggering malignancy in breast cancer, (Phosphatase and tensin homolog) PTEN is known to be a crucia...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of Ultraviolet-B irradiation on the photodegradation of ciprofloxacin and norfloxacin as inhibitors of bacterial DNA replication and cell division in urea medium.
This study aimed to assess the effect of Ultraviolet B (UVB) irradiation in removing ciprofloxacin and norfloxacin by using a simulating model of wastewater contained urea at pH 4. A known concentration of ciprofloxacin and norfloxacin were prepared in an appropriate aqueous solution in presence or absence 0.2M urea and adjusted at pH 4. The dis-solved drugs were irradiated with UVB-lamp in a dark place for 60 minutes. The percent of removal and the rate of elimination (k) of each drug were calculated. The direct photolysis effect of UVB irradiation was observed with ciprofloxacin which amounted to 24.4% removal compared w...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Optimization of fermentation of Fomes fomentarius extracellular polysaccharide and antioxidation of derivatized polysaccharides.
Authors: Zhang Q, Li Y, Zong S, Ye M Abstract Fermentation is a metabolic process that converts sugars into acids, gases, or alcohol. This process occurs in yeasts and bacteria, as well as in muscle cells when faced with a lack of oxygen. In this paper, isolation, culture, purification and extracellular polysaccharides of strain Fomes fomentarius were studied. Extraction of polysaccharides from a culture based on F. fomentarius extracellular polysaccharides, extracellular polysaccharides fermentation experiments was optimized and compared, the optimal fermentation method was obtained; extracellular polysaccharides ...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression.
Authors: Chen L, Zhang S, Liu X, Dai J, Yan Z, Wu C Abstract Brain microvascular endothelial cells are a key part of the blood-brain barrier. This experiment was set up to investigate the effect and molecular mechanism of Dendrobium polysaccharide on oxidized low-density lipoprotein (ox-LDL)-induced damage to the human brain microvascular endothelial cells. For this purpose, human brain microvascular endothelial cells HBMEC were divided into control group (without any treatment), ox-LDL group (50 μg/mL ox-LDL), Dendrobium polysaccharide low, medium and high concentration group (0.1 μg/L, 0.2 μg/L, 0.4 μ...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between TIM-3 gene polymorphisms and steroid-resistant primary nephrotic syndrome in children.
Authors: Lei J, Ma S Abstract Nephrotic syndrome, also known as nephrosis, is a collection of symptoms in medicine and urology caused by damage to the basement membrane of the kidney glomeruli and the kidneys excrete a large amount of protein. This experiment was carried out to investigate the association of three single nucleotide polymorphisms (SNPs) of T-cell immunoglobulin and mucin-domain-containing-3 (Tim-3) with childhood primary nephrotic syndrome (PNS) steroid response in Han Chinese. For this purpose, a total of 218 children with steroid-resistant PNS and 189 children with steroid-responsive PNS were enro...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protein molecular defect detection method based on a neural network algorithm.
Authors: Zheng M, Kahrizi S Abstract Proteins, as the largest macromolecules in the body, are among the most important components of the body and play very vital and important roles. These substances are made up of a series of amino acid chains that, depending on the type of protein, the number of these amino acids can reach several thousand. Proteins function differently depending on the type and location of their presence, including enzymatic activity to catalyze the process, identify microbes and cancer cells, transport substances such as respiratory gases, and signalize. In the biochemical experiment, the probl...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Atomic force microscope observation of athletes' hemoglobin imaging.
Conclusion: It is more accurate and comprehensive to use AFM to observe athletes' hemoglobin. PMID: 33287927 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Design of biological gene information collection system based on data mining technology.
Authors: Wu Y, Mu X, Kahrizi D Abstract At present, bioinformatics research focuses on the development from the accumulation of biological data to the integration and processing of biological data. This paper designs a bio gene information collection system based on data mining technology. In the system, the information of gene web analysis database, data mining model database and gene chip database is transferred to gene algorithm tool library, which can extract, transform and load the biological gene information, transfer the collected and processed biological gene information to gene general chip and web databas...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Design of abnormal data detection system for protein gene library based on data mining technology.
Authors: Liu C, Wang Y Abstract In view of the shortcomings of the current abnormal data detection system of the protein gene library, such as low detection rate and high error detection rate, the abnormal data detection system of the protein gene library based on data mining technology is designed. The protein gene enters the firewall module of the system, and enters the immune module when it does not match the firewall rules; the memory detector in the immune module presents the protein gene, if the memory detector does not match the protein gene, the mature detector presents the protein gene, if the mature detec...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Biosynthesis and characterization with antimicrobial activity of TiO2 nanoparticles using probiotic Bifidobacterium bifidum.
Authors: Hkeem Ibrahem K, Ali FA, Abdulla Sorchee SM Abstract Bifidobacterium selectively colonizes the infants' intestinal tract, and the relevant coliform bacteria in adults are particularly beneficial because of their enhanced capability to prevent pathogens of gastro intestine by direct antimicrobial action and relieve infection, which led to their intensification, the antibacterial activities of titanium nanoparticles producing by some bacteria, makes them attractive as a new agent against pathogenic bacteria. In our present study, we used a probiotic bacteria Bifidobacterium bifidum which was isolated from th...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Quercetin may repair the impaired oxidant-antioxidant balance in 1, 2, 3, 4-tetrachlorodibenzo-p-dioxin induced cardiac injury in male rats.
Authors: Nanakali NM Abstract The purpose of this study was to evaluate the prophylactic effects of quercetin (QUE) against oxidative damages and histological changes induced by 1, 2, 3, 4-tetrachlorodibenzo-p-dioxin (TCDD) in rat heart. For this purpose, 20 adult male Wistar rats were divided into four groups as fellow control group, TCDD group (10µg/kg BW/day by gavage daily for 60 consecutive days), QUE group (30 mg/kg BW/day) and TCDD + QUEgroup. The oxidative biomarkers of heart tissue and the levels of cTnI and lipid profile in serum were measured. Our results showed that the cTnI serum level and the he...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Estimation levels of prostate-specific antigen, interleukin-8, oxidative stress and some inflammatory markers in sera of benign prostatic hyperplasia patients who have smoking habits as a risk factor.
Authors: Al-Barzinj RMGT Abstract Benign prostatic hyperplasia (BPH) is a common multifactorial inflammatory disease of older men, defined by increased growth of prostate epithelial and stromal cells. Elevation serum levels of Interleukin-8 (IL-8), oxidative stress and inflammatory markers represent predictive surrogate markers of the disease progression in smoker BPH patients. A total of 86 BPH patients and 54 control subjects were admitted to the urology unit, Rizgary Teaching Hospital in Erbil City, Iraq between January and June 2020. Sera levels of prostate-specific antigen(PSA), IL-8, malondialdehyde (MDA...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Immunohistochemical expression of ATRX in gliomas.
Authors: Jalal JA, Rowandizy AIS, Ismael AT Abstract Glioma is one of the primary tumors of the central nervous system that occurs in the spinal cord or brain and the origin of the tumor is from glial cell cells. The most common site of glioma tumors is the brain. Glioma accounts for 30% of all central nervous system tumors and 80% of malignant brain tumors. Alpha-thalassemia/mental retardation syndrome X-linked (ATRX) mutations are frequently distinguished in gliomas. Current research is an attempt to assess ATRX immunoexpression in different types of gliomas diagnosed, in Erbil-Iraq, and to evaluate its associati...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Application of intravoxel incoherent motion imaging in the study of migration and movement of cancer cells.
Authors: Wang Y, Xu H, Ahmed IJ Abstract IVIM imaging technology can obtain physiological information of tissue cells and blood perfusion at the same time in one imaging process. Taking female patients in Shenzhen Maternal and Child Health Hospital affiliated to Southern Medical University as an example, the migration and movement of breast cancer cells and hepatocellular carcinoma cells are studied by intravoxel incoherent motion imaging. Apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudo-diffusion coefficient (D*) and perfusion fraction (f) are measured by the IVIM bi-index model, and st...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Analysis of topological properties and drug discovery for bipolar disorder and associated diseases: A bioinformatics approach.
The objective of the present study was to investigate the association between genetic mutations in the four above listed diseases and to create a Protein-protein interaction (PPI) network or common pathways. Firstly, we need to find out the genetic relationship between them. Thus it will help us to understand the genetic association between them and help to develop the drug design for all the diseases. Genes responsible for these diseases are gathered, pre-processed, processed and mining using python scripts. This exploration is expected to carry out further measurements in the field of drug structure and also contributes ...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of atrial fibrillation-related circular RNAs and constructing the integrative regulatory network of circular RNAs, microRNAs and mRNAs by bioinformatics analysis.
Authors: Zhai Z, Qin T, Liu F, Han L, Zhou H, Li Q, Xia Z, Li J Abstract Atrial fibrillation (AF) is the most common cardiac arrhythmia with a high incidence of stroke. Many circular RNAs (circRNAs) have been demonstrated they are elated to various heart diseases and may play important roles in diagnostics or many pathophysiological processes. Nevertheless, there is Few studies on circRNAs functions in persistent AF. To identify AF-related circRNAs and construct the integrative regulatory network of circular RNAs, miRNAs, and mRNAs, we collected human right atrial appendage tissues from 5 patients suffering persist...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential risk assessment of miR-143 gene polymorphisms rs41291957 and rs353292 in colorectal cancer.
Authors: Ahmed MN, Mansoor Q, Baig RM, Tahir T, Abbasi SA Abstract Colorectal cancer is a life-threatening and therapeutically challenging disease. Increasingly it is being deciphered that genetic and epigenetic mutations play a central role in cancer onset and progression. Excitingly, discovery of non-coding RNAs is considered to be a milestone in molecular oncology and emerging evidence is deepening our understanding about pivotal role of miRNAs in carcinogenesis. miR-143 has been experimentally verified to play an instrumental role as tumor suppressor. Recent studies suggest that single nucleotide polymorphisms ...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Raddeanin A suppresses lung cancer cell proliferation via induction of apoptosis and increased production of ROS.
Authors: Jin W, Lu S, Wang X, Shu Y, Shi H Abstract At present, in vitro cell experiments have confirmed that RaddeaninA can effectively inhibit the proliferation of some tumor cells, but the effect of RaddeaninA on lung cancer cells has not been observed. Therefore, this study explored its effect on lung cancer cells and its mechanism of action. Human lung cancer cell lines were treated with serum-free medium and varied concentrations of Raddeanin A. Cell proliferation and apoptosis were determined using MTT, and flow cytometric assays, respectively. The intracellular level of ROS was determined using DCFH-DA assa...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-196a targeting LRIG3 promotes the proliferation and migration of cervical cancer cells.
Authors: Qiu Y, Han Q, Lu H, Shi C Abstract In recent years, studies have found that miR-RNA plays a role in cell proliferation, differentiation, apoptosis and metabolism. Among them, miR-196a is closely related to cervical cancer. Therefore, this experiment investigates the effect of mir-196a expression on cervical cancer cells and related mechanisms. The expression level of miR-196a in the cervical cancer cell line was assayed with the RT-PCR method, and liposome transfection was used to investigate its up-regulation or down-regulation in cervical cancer cells. The CCK-8 method and flow cytometry were used to mea...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Diagnostic value of cfDNA and long fragment DNA in patients with breast cancer.
Authors: Xu H, Liu G Abstract To investigate the diagnostic value of cell-free DNA (cfDNA) and long fragment DNA in breast cancer patients.Female patients with breast cancer (n = 80) were recruited over one year for this study, and served as an observation group. The control group consisted of 50 normal, healthy females. Plasma levels of cfDNA and long fragment DNA were determined a day before treatment, 7 days after treatment, and on the 20th day of treatment. The levels of cfDNA and long fragment DNA in breast cancer patients before treatment were significantly higher than those of the control group (p<0.0...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Fibrillin 2 gene knockdown inhibits invasion and migration of lung cancer cells.
Authors: Hong Q, Li R, Zhang Y, Gu K Abstract To investigate the effect of Fibrillin 2 (FBN2) expression on the invasion and migration of lung cancer cells, and the underlying mechanism. Protein and mRNA expressions of FBN2 were assayed. The relationship between FBN2 protein expression and clinical characteristics of lung cancer patients was analyzed. Correlation between FBN2 expression level and patient survival time was analyzed. Moreover, the mRNA and protein expression of FBN2 in lung cancer cells and human normal lung epithelial cells were assayed. After constructing low-expressing FBN2 cells, the cell prolife...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10.
Authors: Li Y, Chen L, Zhang B, Ohno Y, Hu H Abstract Ovarian cancer is a leading cause of gynecological cancer-related mortality. It has been reported that miR-409-3p is involved in the proliferation and migration of cancer cells. However, the role of miR-409-3p in ovarian cancer has not been well studied. The present study aimed to investigate the functional role of miR-409-3p in the pathogenesis of ovarian cancer, and its potential mechanism. It was found that the expression levels of miR-409-3p in 6 ovarian cancer tissues were upregulated. Through proliferation, migration and colony formation assays, it was rev...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lymphocyte cell population as a potential hematological index for early diagnosis of COVID-19.
In this study, we investigate the potential clinical utility of lymphocyte CPD for early diagnosis of COVID-19. To investigate the potential of lymphocyte cell population data (lymphocyte CPD) for use in early diagnosis of coronavirus disease 2019 (COVID-19). Lymphocyte CPD of healthy control (n = 51), common cold patients (n = 49) and mild COVID-19 patients (n = 126) were generated using hematology analyzer. The parameters were subjected to sensitivity and specificity analysis to determine their suitability as biomarkers for early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Normali...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of regulation and mechanism of miR-223 on autophagy of CD4 + T lymphocytes in septic mice.
Authors: Li Q, Xiang G, Peng C, Xie L, Ji W Abstract T-lymphocyte dysfunction is most important part of immune dysfunction in sepsis, where dynamic change, especially autophagy of CD4+T lymphocytes is found to be related to disease fate. Our study is to i nvestigate the changes of CD4 + T lymphocytes and their autophagy levels in septic miR-223 -/- mouse model injected intraperitoneally with E. coli.120 male C57BL/6J wild-type. Twenty male miR-223 knockout(miR-223-/-) mice were randomly divided into, according to intraperitoneal injection of normal saline (NS) and E. coli solution, normal saline (WT NS) group, seps...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A comprehensive review of histopathological findings of infections induced by COVID-19.
Authors: Balaky STJ, Zaki Abdullah SM, Alexander M, Maashi MS, Alkaim AF, Shahriyari S, Tabari F, Kazemi E Abstract The severe acute respiratory syndrome (SARS)-Coronavirus (CoV2) virus, first identified in Wuhan, China, caused the coronavirus disease 2019 (COVID-19) which soon became a global pandemic, as labelled by the World Health Organization (WHO). The transmission method of the infection is primarily through droplets of various sizes. The SARS-CoV2 virus leads to a severe respiratory illness which in the first place causes the simulation of the acute respiratory syndrome. In order to diagnose of COVID-19 eff...
Source: Cellular and Molecular Biology - December 10, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cisatracurium inhibits the proliferation, migration and invasion of breast cancer cells by regulating the expression of miR-3174.
Authors: Yang X, Ma W Abstract Breast cancer is a type of cancer that begins in the breast tissue. Being a woman is the most important factor in the risk of breast cancer. Although men also get the cancer, women are much more likely to get it. This experiment was founded to investigate the effect and mechanism of Cisatracurium on breast cancer cell proliferation, migration and invasion. Breast cancer cells MDA-MB-231 were cultured in vitro. MDA-MB-231 cells were treated with cisatracurium of different concentrations for 48 h. CCK-8method detected cell proliferation, Transwell detected cell migration and invasion, W...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of Dengzhan Shengmai Capsule combined with butylphthalide soft capsule on oxidative stress indexes and serum Hcy and CRP levels in patients with vascular dementia.
Authors: Ma Y, Sun W Abstract This experiment was aimed to investigate the effect of Dengzhan Shengmai capsule combined with butylphthalide soft capsule on oxidative stress indexes and serum homocysteine (Hcy) and C-reactive protein (CRP) levels in patients with vascular dementia (VD). From July 2017 to July 2019, 123 patients with VD in our hospital were selected as the research object, and each patient was assigned a random number according to the order of treatment. Among them, No. 1 to 41 were the control group A, No. 42 to 82 were the control group B, and No. 83 to 123 were the research group. Control group A ...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Interleukin-6 role in the severity of COVID-19 and intensive care unit stay length.
Authors: Taher Al Barzin RMG, Ghafour Raheem S, Khudhur PK, Abdulkarimi R, Mohammadnejad E, Tabatabaee A Abstract Evaluation of cytokine production in COVIID-19 disease, in which the cytokine storm is one of the most important pathological features in complicated cases, especially interleukin 6 as a pre-inflammatory cytokine that exacerbates the immune response, could help determine the pathophysiology of the disease. Examining the level of this cytokine along with other related factors can help to better understand the pathogenesis of this disease. In this cross-sectional study, 48 patients with COVID-19 whose dis...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigating the changes in the levels of HbA1c, blood fat and insulin sensitivity in elder patients with type II diabetes mellitus due to combined medication of pioglitazone and melbine and single-use of pioglitazone.
This study was founded for the purpose investigate the differences in effects of combined medication of pioglitazone and melbine and single-use of pioglitazone on the levels of hba1c, blood fat and insulin sensitivity of elder patients with type II diabetes mellitus (T2DM), to provide clinical reference and guidance for the treatment of T2DM in elder patients. For this purpose, we selected a total of 120 elder patients with T2DM who visited the clinic or were admitted to this hospital between July 2016 and July 2017 and divided them into the observation group and the control group (n=60 for each group). For the control gro...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Wogonin suppresses proliferation and invasion of skin epithelioid carcinoma cells through Notch1.
In conclusion, wogonin may inhibit skin epithelioid carcinoma cell proliferation and invasion at least partially by repressing the Notch1 gene expression. PMID: 33040781 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Tuling Wendan Decoction combined with flunarizine in the treatment of migraine patients and the effect of intervention on serum cyclooxygenase-2, endothelin-1 and nitric oxide.
Authors: Xie X, Shang K, Li X Abstract This experiment aimed to explore the curative effect of Tuling Wendan Decoction combined with flunarizine on migraine patients and the intervention effect on serum cyclooxygenase-2 (COX-2), endothelin-1 (ET-1), nitric oxide(NO) levels. For this purpose, from January 2019 to January 2020, 96 patients with migraine in our hospital were selected as the research object. Using a simple randomization method, patients who meet the criteria were assigned 1:1, and each patient was assigned a random number, of which the number 1 to 48 were the observation group, and the number 49 to 96 ...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Significance changes in the levels of myocardial enzyme in the child patients with Mycoplasma Pneumoniae Pneumonia.
Authors: Qi X, Sun X, Li X, Kong D, Zhao L Abstract Mycoplasma is a gram-negative with thin wall bacterium that in humans, Mycoplasma pneumoniae causes pneumonia. This experiment was designed to explore the changes of myocardial enzymes in the mycoplasma pneumoniae pneumonia (MPP) child patients, and analyze the clinical value of these changes, in combination with the relevant indicators, symptoms and signs, in the evaluation of the pneumonia mycoplasma infection. For this aim, a total of 120 child patients with MPP in the acute phase,120 child patients with MPP in the recovery phase and 120 healthy children were s...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of Salvia miltiorrhiza and Radix astragali on the TGF- β/Smad/Wnt pathway and the pathological process of liver fibrosis in rats.
Effects of Salvia miltiorrhiza and Radix astragali on the TGF-β/Smad/Wnt pathway and the pathological process of liver fibrosis in rats. Cell Mol Biol (Noisy-le-grand). 2020 Sep 30;66(6):46-51 Authors: Cao T, Lu Y, Zhu M, Cheng J, Ye B, Fang N, Cui Y, Xue B, Lari Najafi M, Kazemi E Abstract Traditional Chinese medicine has made some progress in the study of liver fibrosis, and provides valuable experience for clinical treatment of liver fibrosis. The aim of this study was to investigate the rationality of compatibility use of Salvia miltiorrhiza and Radix astragali on liver fibrosis in rats. For t...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway.
Authors: Ren S, Qing S Abstract Oral squamous cell carcinoma is one of the most common high malignant tumors. This experiment aimed to investigate whether ethyl acetate extract of peony (Paeonia suffruticosa) seed coat could affect the proliferation and apoptosis of oral squamous carcinoma cells by regulating the miR-424-3p/STAT3/Survivin pathway. For this purpose, oral squamous cell carcinoma cell CAL27 was cultured in vitro, and cells were treated with ethyl acetate extract of peony seed coat at different concentrations. MTT was used to detect cell proliferation. Flow cytometry was used to detect apoptosis. qRT-P...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiRNA-214 promotes the pyroptosis and inhibits the proliferation of cervical cancer cells via regulating the expression of NLRP3.
Authors: Yu S, Zhao N, He M, Zhang K, Bi X Abstract Inflammasome mediates the maturation of interleukin-1β (IL-1β) and IL-18, triggers the pyroptosis and associates with multiple autoimmune diseases. In light of this, we hope to investigate the regulatory role of miRNA-214 in the inflammasome of cervical cancer. With the samples collected from 50 cervical cancer patients and 50 age-matched healthy subjects, real-time PCR and Western blotting were employed to detect the mRNA and/or protein expression profiles of the NOD-like receptor protein family, including NLRP1, NLRP3, NLRC4, Caspase-1, IL-1β, IL-...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect and mechanism of rapamycin on proliferation and apoptosis of human lung cancer cells.
In conclusion, RAPA can induce apoptosis of NCI-H446 cells by down-regulating Bcl-2 gene expression, up-regulating P53 and Bax gene expression, and increasing intracellular Ca2+ concentration and its apoptosis induction effect have timeliness and dose-effect. PMID: 33040787 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of latanoprost on the expression of TGF- β1 and Wnt / β-catenin signaling pathway in the choroid of form-deprivation myopia rats.
In this study, we investigated the effect of latanoprost on the expression of TGF- β1 and Wnt / β - Catenin signal pathway in the choroid of form-deprivation myopia model rats. Forty rats were randomly divided into two groups: the control group and the FDM model group. Each group had 20 rats. The FDM model group was established by feeding latanoprost daily for 28 days. 15 rats in each group were used to measure the length of the ocular axis and the level of TGF-β1 in choroidal tissue; the remaining 5 rats in each group were used for choroidal fibroblast culture. After modeling, the rats were killed, the leng...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long noncoding RNA H19 promotes vincristine resistance in multiple myeloma by targeting Akt.
Authors: Wang Y, Xu S, Wang L, Liu J, Wang J, Zhou J Abstract Multiple myeloma is a malignant proliferation of plasma cells that results from a single clone. Manifestations include bone pain or fractures, kidney failure, susceptibility to infection, anemia, and hypercalcemia. To investigate the relationship between vincristine (VCR) resistance and Long noncoding RNA H19 (Lnc-RNA H19) in multiple myeloma (MM) this experiment was set up. For this aim, RT-PCR was used to detect the expression of lnc-RNA H19 in 60 MM patients from No.215 Hospital of Shaanxi Nuclear Industry and 50 healthy controls, and further detected...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of Twist1 on drug resistance of chronic myeloid leukemia cells through the PI3K/AKT signaling pathway.
In conclusion, Twist1 and the PI3K/AKT signaling pathway are over-activated during the formation of the CML cells resistant to imatinib. The silence of Twist1 can reverse the resistance through the pathway. PMID: 33040790 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Diversity and distribution of bacteria and archaea in Tuosu Lake in Qaidam Basin.
Authors: Song W, Sun C Abstract Microbes in plateau lakes are important participants of material circulation and energy flow in plateau ecosystems. Knowledge of the microbiota, such as bacteria and archaea, community distribution and diversity in plateau lakes is the basis to understand the species succession, adaptation, maintenance and metabolic mechanisms of specific environmental microbial ecosystems. This work aimed to reveal the diversity and# succession of the microbiota in Tuosu Lake to provide a biological basis for the exploration and development of microbial resources in the plateau lakes. The distributi...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Exploration and analysis of the value of tumor-marker joint detection in the pathological type of lung cancer.
Authors: Gao S, Zhang G, Lian Y, Yan L, Gao H Abstract Lung cancer is a disease characterized by the uncontrolled growth of cells in lung tissue. If left untreated, cell growth can spread beyond the lungs to a process called metastasis and reach surrounding tissues or other organs. This experiment was set up to discuss and analyze the research value of joint detection of tumor markers including carcino-embryonic antigen (CEA), cytokeratin 19 fragments (CYFRA21-1) and neuron-specific enolase (NSE) in the diagnosis and pathological type of lung cancer. From November 2016 to February 2018, 378 cases of patients with l...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lowered Cyclin E levels increase the efficiency and the specificity of capsaicin against cancerous cells of mesothelium.
In this study, we investigated the anti-mesothelioma potential of capsaicin by observing its cytotoxicity on healthy, immortalized and cancerous cells of mesothelium in vitro and how this potential be affected by lowered Cyclin E levels, a key regulator of G1/S transition of cell cycle. For this purpose, we determined and compared the IC50 values of capsaicin in both FBS (Fetal Bovine Serum) containing and FBS-deprived medium of each cell population studied. Additionally, we examined the changes in both protein and mRNA levels of caspase-3 upon capsaicin exposure as well as conducted a series of experiments through which t...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Calcium based siRNA coating: a novel approach for knockdown of HER2 gene in MCF-7 cells using gold nanoparticles.
Authors: Sami A, Naqvi SMS, Qayyum M, Raza Rao A, Sabitaliyevich UY, Ahmad MS Abstract Surface functionalization of nanoparticles (NPs) for therapeutic siRNA delivery into cancer cells has gained interest. The present study was designed for surface functionalization of gold nanoparticles (AuNPs) for efficient siRNA delivery and knockdown in cancer cells. In order to achieve this objective, AuNPs were coated with HER2-siRNA in the presence of 11-mercaptoundecanoic acid (11-MUA), calcium chloride (CaCl2) and polyethyleneimine (PEI) in alternate charge bearing successive layers. MCF-7 cells were cultured and transfect...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research