The synthesis of non-structural carbohydrates under the network security model.
Authors: Chai X, Li Y Abstract In order to explore the pathway of non-structural carbohydrate synthesis, an analysis of the pathway of non-structural carbohydrate synthesis under the network security model was proposed. Taking non-structural carbohydrates as the research object, the experimental materials and equipment were selected under the network security model. Through the establishment of detection methods, the preparation of freeze-dried carbohydrates, the influence of synthesis pathway-specific inhibitors on the synthesis of non-structural carbohydrates, the influence of precursors and intermediates in the ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The expression and significance of NF- κB, MMP1, and MMP2 in rats with abdominal aortic aneurysm.
This study aimed to investigate the expression and significance of NF-κB, matrix metal protein1 (MMP1), and matrix metal protein2 (MMP2) in rats with abdominal aortic aneurysm (AAA). 48 Wistar rats were collected, then they were divided into an experiment group and a control group (n=24). The rats in the experiment group were used to carry out an abdominal aortic aneurysm modeling, while the rats in the control group were infused with a same dose of saline through catheters. qRT-PCR and ELISA were used to detect the expressions of EGF and VEGF-C in skin tissues and mRNA of NF-κB, MMP1, and MMP2 in their abdomin...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-92b as a marker for TPF induced chemotherapy response prediction and prognosis evaluation in with advanced oral squamous cell carcinoma patients.
This study aimed to explore the clinical value of miR-92b in advanced oral squamous cell carcinoma (OSCC) and to observe the relationship between miR-92b and TPF induced chemotherapy and prognosis. Totally 114 patients with advanced OSCC admitted to our hospital were selected as the study subjects, all of whom received docetaxel, cisplatin and 5-fluorouracil (TPF) induction chemotherapy. In addition, another 80 healthy subjects who underwent physical examination in our hospital from the same period were enrolled. The serum expression of miR-92b was detected by qRT-PCR. Serum miR-92b was up-regulated in patients with advanc...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Influenced CD cells and ICAM-1 by pulmonary surfactant combined with high-frequency oscillatory ventilation and its effects on immune function in children with neonatal respiratory distress syndrome.
This study aimed to explore the clinical efficacy of pulmonary surfactant combined with high-frequency oscillatory ventilation (HFOV) on neonatal respiratory distress syndrome (NRDS) and its influence on immune function in children. Children admitted to our hospital from March 2017 to March 2019 who received HFOV combined with pulmonary surfactant therapy as a research group. Sixty-two children received conventional nasal continuous positive pressure combined with pulmonary surfactant therapy as a control group. Clinical efficacy, blood gas and immune function of patients were compared between the two groups. The clinical ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Correlation of MMP-9 and HMGB1 expression with the cognitive function in patients with epilepsy and factors affecting the prognosis.
This study was designed to investigate the expressions and roles of MMP-9 and HMGB1 in peripheral blood of patients with epilepsy and their relationship with the cognitive function and to explore factors affecting the prognosis of epilepsy patients. A total of 127 patients with epilepsy were collected in the study group and 120 healthy subjects receiving a physical examination at the same time were collected in the control group. The MMP-9 and HMGB1 expressions and their diagnostic value for epilepsy were compared between the two groups. The relationship between MMP-9 and HMGB1 expression levels and the clinical-pathologic...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Uninfluenced alpha-fetoprotein and treatment of liver primary carcinoma by lobaplatin in combination with 5-fluorouracil and doxorubicin via chemoembolization and transarterial chemoembolization.
Authors: Chen Y, Li W, Yang Q, Chen L, Zeng C Abstract Alpha-fetoprotein (AFP) is a protein encoded by the AFP gene and normally produced by the fetus. The purpose of this study is to investigate the efficacy of lobaplatin in combination with 5-fluorouracil (5-FU) and doxorubicin on AFP and treatment of primary carcinoma of the liver by transhepatic arterial chemotherapy and embolization (TACE). Patients with primary carcinoma of the liver who took the TACE for treatment were enrolled in this study and divided randomly into the research group and the control group. Patients in the research group adopted the TACE in...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Parenteral nutrition effects of Omega-3 fatty acids on C-reactive protein, high-density lipoprotein, lymphocyte characteristics and the treatment of critically ill patients.
Authors: Ni C, Cao J, Li D, Wu W, Cao L, Zhu C Abstract To study the effects of omega-3 fatty acid parenteral nutrition on the nutrition, inflammatory responses, immunity and prognoses of critically ill cancer patients. A total of 80 critically ill cancer patients were randomly divided into an observation group and a control group, 40 cases in each group. Both groups of patients received equal-nitrogen and equal-calorie enteral and parenteral nutrition. The observation group, on this basis, was added with omega-3 fatty acid parenteral nutrition. The weekly nutritional status measures, inflammatory response measures...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of a genetic variant in the AKT gene locus and cardiovascular risk factors.
Authors: Kermanshahi F, Ghazizadeh H, Hussein NA, Amerizadeh F, Samadi S, Tayefi M, Kamel Khodabandeh A, Moohebati M, Ebrahimi M, Esmaily H, Sahebi R, Abdulkarimi R, Mousavi SAR, Ferns GA, Avan A, Ghayour-Mobarhan M Abstract Cardiovascular disease (CVDs) is the leading cause of morbidity and death worldwide. Most genetic variants could be identified by several genome-wide-association-studies (GWAS), including within genes encoding proteins involved in the AKT/PI3K pathways that are related with an increased risk of metabolic syndrome and CVDs. Therefore, due to the importance of genetic variants in the prognosis of...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comprehensive multi-factors reveal the pathogenesis of degenerative intervertebral disc.
In conclusion, we recognized the ncRNA pivot (including miR-193b-3p, CRNDE, etc.) and TF pivot (including E2F1, E2F4, etc.) that significantly regulate dysfunction modules. PMID: 32538749 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The expression of lncRNA-MALAT1 in breast cancer patients and its influences on prognosis.
This study aimed to explore the expression of lncRNA-metastasis associated lung adenocarcinoma transcript 1 (lncRNA-MALAT1) in breast cancer (BC) patients and its influences on the prognosis of the patients. A total of 120 BC patients admitted to our hospital were enrolled as a BC group, of which 58 patients at I/II stage were treated with breast-conserving surgery as an operation group, and the other 62 patients at III/IV stage were treated with neoadjuvant chemotherapy combined with breast-conserving surgery as a combination group. Meantime, 100 healthy people in physical examination during the same period were enrolled ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of miR-27a-3p-mediated Smurf2 on bleomycin A5-induced pulmonary fibrosis in rats.
This study aimed to explore the effects of miR-27a-3p-mediated Smurf2 on bleomycin A5-induced pulmonary fibrosis in rats. Sixty clean-grade SD rats were made into models of pulmonary fibrosis induced by bleomycin A5. They were randomly divided into the control group (fed as usual), the bleomycin A5 group, and the miR-27a-3p group according to the modeling. Pathological sections and morphological observations were performed on the lung tissues of all rats, and the expression of miR-27a-3p, Smurf2 mRNA, Smurf2 protein, collagen type I (Col I), collagen type III (Col III), and related inflammatory factors in lung tissues were...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression and prognosis of CyclinA and CDK2 in patients with advanced cervical cancer after chemotherapy.
This study aimed to investigate the expression and prognosis of CyclinA and CDK2 in patients with advanced cervical cancer after chemotherapy. The patient history of 108 patients with advanced cervical cancer admitted to our hospital from December 2013 to January 2016 was selected as a cervical cancer group. 54 normal healthy people admitted to our hospital for physical examination in the same period were selected as the control group. Western blotting and RT-PCR were used to detect the difference between CyclinA and CDK2 proteins and mRNA expression between the two groups and the correlation between them was analyzed. The...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression and clinical diagnostic value of miR-383 in patients with severe preeclampsia.
This study aimed to investigate the expression and clinical diagnostic value of miR-383 in patients with severe preeclampsia. Thirty patients with severe preeclampsia from July 2017 to December 2018 were selected as a research group, twenty healthy pregnant women undergoing physical examination at the same period were selected as a control group, and miR-383 and miR-16 in placenta tissue of the two groups were detected by qRT-PCR. ROC curve was drawn to evaluate the predictive value of diagnostic efficiency, Spearman test was used for correlation analysis, and Logistic univariate and multivariate analysis was performed on ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Verification of expression of LINC00648 in the serum of lung cancer patients by TCGA database.
Authors: Zhao L, Gao M, Zhao X Abstract TCGA data were used to verify the expression of LINC00648 in lung cancer patients to provide a reference for clinical practice.  Lung cancer transcriptome data were downloaded by the TCGA database and LINC00648 data were extracted for analysis. Fifty-two patients with lung cancer diagnosed in our hospital from May 2014 to March 2016 were collected as the patient group and 30 normal people as the control group. RT-qPCR was used to detect the expression of LINC00648 in serum, follow up of patients was carried out, and bioinformatics was used to analyze the potential mechan...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bazedoxifene effects on osteoprotegerin, insulin-like growth factor, tumor necrosis factor and bone mineral density.
CONCLUSION: Bazedoxifene is an effective drug for the treatment of postmenopausal osteoporosis. It can not only prevent the rapid loss of bone mass, effectively relieve the symptoms of menopause, but also improve bone density and reduce the risk of fracture. PMID: 32538755 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of IgG, IgM, CD4+ and CD8+ T cells during neoadjuvant chemotherapy with Tezio and Apatinib in gastric cancer patients.
Authors: Cui Z, Zhang J, Zhang J, Xu L Abstract This experiment was carried out to study the effect the neoadjuvant chemotherapy with Tezio and Apatinib on the treatment of advanced gastric cancer and its impact on the postoperative immune function of patients' function. The patients with advanced gastric cancer who were treated in gastrointestinal surgery in our hospital from January 2017 to December 2018 were divided into two groups. The new chemotherapy and the traditional chemotherapy groups. The new chemotherapy group received neoadjuvant chemotherapy of Tezio combined with Apatinib before surgery, and the con...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Patrinia herba aqueous extract on the proliferation, apoptosis, invasion and migration of hepatocellular carcinoma cells.
This study aims to study the effect and mechanism of Patrinia herba aqueous extract on proliferation, apoptosis, invasion and migration of hepatocellular carcinoma cells. Hepatocellular carcinoma cells MHCC97-H were treated with 2.5, 5, 10 mg/mL P. herba aqueous extract, cell counting kit 8 (CCK-8), flow cytometry, plate cloning experiments, and Transwell measured cell survival, apoptosis, colony formation, invasion, and migration, respectively. Real-time quantitative PCR (qPCR) and western blot were used to detect long non-coding RNA (lncRNA) HTR2A-AS1 and expression of proteins P21, Caspase-3, E-cadherin and matrix metal...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Correlation of serum BNP and ET-1 levels with cardiac pump function and ventricular remodeling in patients with heart failure.
This study aimed to explore the correlation of serum brain natriuretic peptide (BNP) and endothelin-1 (ET-1) levels with cardiac pump function and ventricular remodeling in patients with heart failure. Eighty-one patients with chronic heart failure admitted to our hospital from March 2016 to November 2018 were enrolled as the study group, and 80 healthy individuals as the control group. Immunofluorescence was used for the detection of serum BNP, ELISA for serum ET-1, and ultrasound for related indexes of cardiac pump function and ventricular remodeling. Moreover, correlation analysis and prognostic factors analysis were ca...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of the expression levels of TNF- α, IL-6, hsCRP and sICAM-1 inflammatory factors, bone mineral density and nutritional status between diabetic and normal pregnant women.
Comparison of the expression levels of TNF-α, IL-6, hsCRP and sICAM-1 inflammatory factors, bone mineral density and nutritional status between diabetic and normal pregnant women. Cell Mol Biol (Noisy-le-grand). 2020 Jun 05;66(3):132-137 Authors: Wang Q, Wang C Abstract To compare the ultrasound bone mineral density, nutritional status and inflammatory state of pregnant women with gestational diabetes mellitus (GDM) and those with normal blood glucose. Retrospective analysis of pregnant women who were prenatally examined and delivered in The 5th People's Hospital of Ji'nan from May 2015 to July 2...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of diagnostic efficacy of MRI and PET/CT in lung cancer of mouse with spinal metastasis.
This study aimed to compare the diagnostic efficacy of MRI and PET/CT in lung cancer of mouse with spinal metastasis. 40 healthy Balb/c nude mice were selected. 0.1 ml of human lung cancer cell A549 bacterial suspension was injected by the left ventricle injection method to establish a lung cancer spinal metastasis model, and the abnormal signs of the nude mice were closely observed. When the body weight was reduced by 20%, micro PET/CT imaging and small coil MRI imaging were applied after intraperitoneal injection of thiopental anesthesia in nude mice. After the imaging was completed, the nude mouse was dissected and the ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

LncRNA NORAD targets miR-410-3p to regulate drug resistance sensitivity of osteosarcoma.
Authors: Xie X, Liu W, Duan Z, Li X, Zhang L, Yang G Abstract The current experiment was performed to investigate the effect of LncRNA NORAD on the sensitivity of miR-410-3p to drug resistance of osteosarcoma cells. The cisplatin-resistant cell line HOS/DDP was induced; si-NC, si-NORAD, miR-NC, miR-410-3p, pcDNA-NC, and pcDNA-NORAD were transfected into HOS/DDP cells, respectively; record as a si-NC group, si-NORAD group, miR-NC group, miR-410-3p group, pcDNA-NC group, pcDNA-NORAD group; si-NORAD was co-transfected into HOS/DDP cells with anti-miR-NC, anti-miR-410-3p, recorded as an anti-miR-NC+si-NORAD group and a...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of dezocine and ropivacaine infiltration anesthesia on cellular immune function indicators, anesthesia recovery time and pain factors in patients with open liver resection.
Authors: Zhu R, Du T, Gao H Abstract The current experiment was carried out to explore the effects of dezocine combined with ropivacaine infiltration anesthesia on the anesthesia recovery time and pain factors of patients with open hepatectomy. A prospective randomized controlled method was used to select 92 patients with open liver cancer resection in our hospital from August 2017 to November 2019. The patients were divided into a study group (n=46) and a control group (n=46) using a computer-generated random number table. Both groups underwent general anesthesia, based on this, the study group was treated with ro...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Decursin inhibits the oxidation of low-density lipoprotein and protects human aortic endothelial cells against oxidative damage.
Authors: Wang X, Yu J, Ji B, Yang Y Abstract Atherosclerosis is the pathological basis of cardiovascular diseases (CVDs) which are the leading cause of death worldwide. The pathogenesis of atherosclerosis itself is complex. Low-density lipoprotein (LDL) oxidation has been shown to increase lipid peroxide levels in arterial wall of atherosclerosis lesion site. Decursin is a coumarin with a range of pharmacological effects. The present study investigated the inhibitory effect of decursin on LDL oxidation, and its protective effect against oxidative damage in human aortic endothelial cells (HAECs). Two models of oxida...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Down-regulation of miRNA-196b expression inhibits the proliferation, migration and invasiveness of HepG2 cells while promoting their apoptosis via the PI3K/Akt signaling pathway.
Authors: Xu F, Zhu F, Wang W, Gao W, Chen X, Yu C Abstract The purpose of this study was to investigate the effects of microRNA-196b (miRNA-196b) on proliferation, migration, invasiveness and apoptosis of hepatocellular carcinoma cell line (HepG2), and the mechanism involved.   MiRNA-196b inhibitor or negative control were transfected into HepG2 cells, while empty liposome vector was used as normal control. The results of transfection were assessed using real-time quantitative polymerase chain reaction (qRT-PCR). Cell proliferation, migration, invasiveness and apoptosis were determined using cell counting...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Electroacupuncture ameliorates post-traumatic stress disorder  in rats via a mechanism involving the BDNF-TrkB signaling pathway.
Electroacupuncture ameliorates post-traumatic stress disorder in rats via a mechanism involving the BDNF-TrkB signaling pathway. Cell Mol Biol (Noisy-le-grand). 2020 Jun 05;66(3):165-170 Authors: Li M, Xie Y, Niu K, Li K Abstract Post-traumatic stress disorder (PTSD) is a mental health condition triggered by a terrifying event, causing flashbacks, nightmares and severe anxiety. It develops in individuals who have experienced a shocking, scary, or dangerous event. Electroacupuncture is reported to be effective for the treatment of PTSD. The present study was carried out to investigate the prot...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Analgesic action of Rubimaillin in vitro and in vivo.
In this study, the analgesic effect of Rub, and its mechanism of action were investigated using glacial acetic acid-induced mice writhing model and a mice model of neurogenic and inflammatory bipolar pain. Analgesic effects were measured in different experimental groups. In vitro, RAW 264.7 cells were used to investigate the release of nitric oxide (NO), iNOS and COX-2 protein in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). The results revealed that Rub reduced the number of acetic acid-induced writhing in mice, inhibited formalin-induced biphasic pain response, and suppressed the production of NO in RAW 264.7...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nitric oxide donor andrographolide enhances humoral and cell-mediated immune responses.
Authors: Zhang B, Yang B, Du L, Guo Y Abstract The present study was aimed to investigate the regulatory effect of Nitric oxide  donor andrographolide (Q-1) on cellular immunity in patients with chronic hepatitis B. Peripheral blood mononuclear cells (PBMCs) were isolated from patients with chronic hepatitis B. Cell viability was assessed using 3‑(4,5‑dimethyl‑thiazol‑2‑yl)‑2,5‑diphenyl‑2H‑tetrazolium bromide (MTT) assay. The levels of expression of interferon gamma (IFN-γ), interleukin 4 (IL-4), interleukin 10 (IL-10) and tumor necrosis factor α (TFN-α) in PBMCs of patients...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Piceatannol inhibits proliferation and induces apoptosis of bladder cancer cells through regulation of the PTEN/AKT signal pathway.
This study was aimed to investigate the effect of piceatannol (PIC) on the proliferation and apoptosis of bladder cancer cell line EJ, and the underlying mechanism.   Bladder cancer cell line EJ was incubated with different concentrations of PIC, and CCK-8 method was used to determine the effect of the treatment on cell proliferation. The effect of PIC on cell cycle, apoptosis and the expressions of related signal pathway proteins were determined using Western blotting. Flow cytometry showed that PIC inhibited the proliferation of EJ cells in a concentration- and time-dependent fashion. Moreover, EJ cells were si...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Luteolin affects keloid fibroblast proliferation and apoptosis by regulating FRAT1 gene expression.
Authors: Zhang X, Liu W, Wei S Abstract The current experiment was performed to investigate whether luteolin affects the proliferation and apoptosis of keloid fibroblasts by regulating the expression of FRAT1 gene. Keloid fibroblasts were treated with luteolin at different concentrations. MTT method, western blot, flow cytometry, and real-time quantitative PCR (qPCR) were used to detect cell proliferation, cyclin D1 (CyclinD1), p21, B-cell lymphoma / leukemia-2 (Bcl-2), Bcl-2 related X protein (Bax), FRAT1 protein expression, apoptosis and ARHI mRNA expression. Keloid fibroblasts were transfected with si-FRAT1, or ...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of lncRNA KCNQ1OT1 on autophagy and drug resistance of hepatocellular carcinoma cells by targeting miR-338-3p.
Authors: Zhong W, Dai Q, Huang Q Abstract The current experiment aimed to investigate the effects of lncRNA KCNQ1OT1 on the proliferation, autophagy and drug resistance of hepatocellular carcinoma cells, as well as the potential molecular mechanism. Hepatocellular carcinoma SK-HEP-1 cells and DDP resistant SK-HEP-1/DDP cells were treated with cisplatin (DDP) of different concentrations (1 nmol/L, 2 nmol/L, 4 nmol/L, 8 nmol/L, 16 nmol/L). The survival rate of SK-HEP-1 and SK-HEP-1/DDP cells was determined by the CCK8 method. QRT-PCR was used to detect the levels of lncRNA KCNQ1OT1 and miR-338-3p in normal hepatocyte...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of some cellular biomarker proteins, oxidative stress and clinical indices as results of laparoscopic appendectomy for perforated appendicitis in children.
Authors: Liu Q, Xu K Abstract Laparoscopic appendectomy for perforated appendicitis in children has the advantages of quick recovery, little influence of inflammatory and oxidative stress and low infection rate. Altogether 115 children with perforated appendicitis treated in our hospital from June 2018 to August 2019 were selected and divided into two groups according to different treatment methods. Laparoscopic appendectomy was used as the research group (RG) (67 cases) and open appendectomy (48 cases) as the control group (CG). The clinical indexes (operation time, intraoperative blood loss, ambulation time, inci...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Construction of SIRT1 gene shRNA lentivirus vector and its effect on the proliferation of breast cancer cells.
Authors: Yue Y, He L, Tian M, Li X Abstract Current experiment aimed to investigate the construction of the SIRT1 gene shRNA lentivirus vector and its effect on proliferation of breast cancer cells. Altogether 80 cases of breast cancer tissues and 80 cases of normal adjacent tissues were collected. qPCR was used for detecting SIRT1 expression. Western blot was used to detect the expression of EMT marker protein. The effect of lentivirus infected sh-SIRT1 on the cell biological function of SK-BR-3 and MDA-MB-231 cells was detected. MTT assay was used to detect cell activity, Transwell cell was used to detect cell in...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic biomarkers: Potential roles in cancer diagnosis.
Authors: Khailany RA, Aziz SA, Najjar SM, Safdar M, Ozaslan M Abstract Biomarkers are indicators of pathogenic processes, typical biological processes, or pharmacological reactions to a therapy. It has several potential usages in cancer; differential diagnosis, prognosis, risk assessment, therapeutic response, and monitoring of disease progression. Recently, advances in oncomarkers raised significant opportunities for enhancing management of cancer. Chromosomal aberration, molecular impairment and epigenetic alteration might be applied to diagnose and prognose cancer and its epidemiology. Some oncomarkers are speci...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TRAIL mediated signaling as a double-edged sword in pancreatic cancer: Analysis of brighter and darker sides of the pathway.
Authors: Farooqi AA, Zahid R, Maryam A, Naureen H, Attar R, Sabitaliyevich UY, Konysbayevna KK Abstract Genetic, genomic and proteomic studies have refined our concepts related to underlying mechanisms of pancreatic cancer. Increasingly sophisticated knowledge has started to shed light on the fact that pancreatic cancer harbored multiple epigenetic and genetic alterations and revealed complicated and dense tumor microenvironments. Our rapidly evolving knowledge about pancreatic cancer has helped us in identification of myriad of underlying mechanisms which play instrumental role in disease onset, drug resistance an...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Combating sars-cov-2 through lipoxins, proteasome, caveolin and nuclear factor- κb pathways in non-pregnant and pregnant populations.
Combating sars-cov-2 through lipoxins, proteasome, caveolin and nuclear factor-κb pathways in non-pregnant and pregnant populations. Cell Mol Biol (Noisy-le-grand). 2020 Jun 05;66(3):221-229 Authors: Celik O, Celik N, Aydin S, Baysal B, Aydin S, Saglam A, Gursu Y, Dalkilic S, Ulas M, Ozcil MD, Tayyar AT, Cengiz F, Ugur K, Akkoc RF, Ersahin AA Abstract It can be misleading to think that the new severe acute respiratory syndrome coronavirus (SARS-CoV2) which has a very strong mutation and adaptation capabilities, uses only the angiotensin-converting enzyme II (ACE2) pathway to reach target cells. D...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clinical effects of Lactobacillus strains as probiotics in the treatment of irritable bowel syndrome. Results from the LAPIBSS trial: Future objectives.
The objective of this communication is to present and analyze the recent results from the LAPIBSS study in order to improve future clinical trials on the effects of Lactobacillus strains in the treatment of irritable bowel syndrome (IBS). Using a tightly-controlled clinical trial protocol with the highest Jadad score of 5/5, the current trial aimed to demonstrate the efficacy of a 2-strain mixture of Lactobacillus acidophilus (L. acidophilus) to improve IBS symptoms. Eighty patients diagnosed with IBS according to Rome III criteria were recruited to a multicentric, double-blind, in parallel groups, placebo-controlled, rand...
Source: Cellular and Molecular Biology - June 18, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Extraction and identification of chemical and molecular compounds of Rosa damascene Mill) essence in different genotypes cultivated in some provinces of Iran.
Authors: Shahbazi K, Assareh MH, Rezaee M Abstract The Rosa damascena Mill. is cultivated in different parts of Iran. For evaluation of its essence, the experiment was carried out on 50 accessions during 2014-17 using randomized complete blocks design with three replications. Results showed that the essence of Kermanshah11 (0.412 g), Yazd 1 (0.400 g), Isfahan 4 (0.364 g), Lorestan 1 (0.361 g), Kermanshah 2 (0.350 g) and Isfahan 3 (0.331 g) accessions had a higher amount of essence. Strong positive correlation coefficients (p≤0.01) were found between essence of citronellol- n-eicosan (0.845), citronellol- n-henei...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synovial and pulmonary dysfunctions are induced by crosstalk of Smad and Erk pathways in an arthritis model.
Authors: Lei W, Jian L, Chuanbing H, Xi C, Lei L, Tianyang L, Yao G, Haixia F, Lei Z, Zhneg L Abstract In the current experiment, the effects of transforming growth factor (TGF)-β1/Smad and ERK pathway crosstalk on synovial and pulmonary systems during rheumatoid arthritis have been investigated. For this purpose, rats were divided into normal control (NC) and model control (MC) groups. In the MC group, 0.1 ml Freund's complete adjuvant was injected intradermally into the right hind paw, and the resulting inflammation represented a rheumatoid arthritis model. Joint swelling and changes in lung functions were o...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of calcitriol combined with sevelamer carbonate on serum parathyroid hormone in patients with chronic renal failure.
This study aimed to observe and analyze the effect of calcitriol combined with sevelamer carbonate on serum parathyroid hormone in patients with chronic renal failure. This study included 180 patients who had been treated for chronic renal failure in our hospital were enrolled as research objects. The patients were randomly divided into two groups: a research group and a control group, each containing 90 cases. The research group was treated with calcitriol combined with sevelamer carbonate, and the control group was treated with calcitriol alone. The therapeutic effects of the two groups were observed and analyzed by...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cancer-associated fibroblasts induce immunotherapy resistance in hepatocellular carcinoma animal model.
Authors: Yu L, Liu Q, Huo J, Wei F, Guo W Abstract Hepatocellular carcinoma is known to be a common predominant cancer in adults, especially in eastern countries. Immune response and cancer-associated fibroblasts (CAFs) have significant influences on tumor development. However, the interaction between CAFs and immunotherapy is unclear in hepatocellular carcinoma. We measured the number of activated fibroblasts in hepatocellular carcinoma samples and samples taken from normal liver tissues. A total of 20 patients' fresh hepatocellular carcinoma and normal tissues which were surrounding the tumor were obtained from t...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression of miR-93-5p in patients with esophageal carcinoma and its relationship with the curative effect and prognosis of radiotherapy.
Conclusion, the expression of miR-93-5p was high in esophageal carcinoma patients, and the higher the expression, the worse the curative effect of radiotherapy and the worse the prognosis, which may be a new predictor of radiotherapy effect and prognosis in patients with esophageal carcinoma. PMID: 32415925 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Integrated transcriptome and microRNA profiles analysis reveals molecular mechanisms underlying the consecutive monoculture problem of Polygonatum odoratum.
This study provided the expression regulation of genes related to CMP of P. odoratum but also suggested that CMP may result in the serious damage of pathogens to roots and cause the slow growth in the consecutive cropping plants. PMID: 32415926 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of AhR and Foxo1 in skin inflammation in burn animal model via MAPK signaling pathway.
Authors: Li K, Fan Y, Xu Z, Xu C Abstract Burn generally refers to thermal damage, including tissue damage caused by hydrothermal (water, soup, oil, etc.), flame, steam, high-temperature gas, hot metal liquid or solid (such as molten steel, ingot). However, little is known about the pathogenesis and inducement of skin inflammation in burned rats. Therefore, this study has carried out an in-depth analysis of the related causes of skin inflammation in burned rats. We analyzed the gene expression and the differentially expressed genes co-expression in burned rats. Subsequently, a set of functional dysfunction modules ...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Microarray analysis of preterm preeclampsia.
Authors: Liu H, Yu J, Yuan W, Shi H, Guan W Abstract Premature preeclampsia is the second cause of maternal mortalities around the world. To investigate its potential driving mechanism(s), we constructed a multi-regulatory-mediated preeclampsia dysfunction module. Through combining differential expression analysis, co-expression analysis, and enrichment analysis, we obtained 23 sets of preeclampsia expression disorder modules in the disease, which involve the modular aggregations of 3016 genes. The modules were subjected to be analyzed for GO and KEGG paths for enrichment analysis. Based on these pivotal regulators...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long-term intervention of taurocholic acid over-expressing in cholestatic liver disease inhibits the growth of hepatoma cells.
Authors: Zhang X, Nan, Zha C, He G, Zhang W, Duan Z Abstract Bile acids usually build up in patients with cholestatic liver disease. It was found that the concentration of taurocholic acid (TCA), one of the taurine conjugates of primary bile acids in serum, was elevated the most. While the role played by TCA in the disease is unclear, there is concern whether TCA contributes to the development of hepatocarcinoma from cholestasis. In the present study, the cell viability, flow cytometry, real-time polymerase chain reaction, intracellular ROS measurement, and intracellular Ca2+ measurement were used to investigate th...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mesenchymal  stromal cells in adipose tissue of breast cancer patients: a cross-sectional study.
Mesenchymal stromal cells in adipose tissue of breast cancer patients: a cross-sectional study. Cell Mol Biol (Noisy-le-grand). 2020 May 15;66(2):71-73 Authors: Gong C, Wang J, Cheng Q, Ren W, Bai J Abstract Mesenchymal stromal cells (MSCs) are important components of the tumor microenvironment and are believed to facilitate cancer growth affecting cancer cells. We investigated this issue in breast cancer patients. In this cross-sectional study biopsy or mastectomy specimens from breast cancer patients who underwent surgery were evaluated based on the presence of MSCs. Flow cytometric analysis was...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of black and green tea extracts on some bacteria-infected burns skin in rats.
Authors: Sorchee SM Abstract Herbal teas are the most common natural alternatives for the treatment of infectious diseases due to increasing antibiotic resistance, and are increasingly gaining more popularity and are known for their activity of antibacterial to many pathogenic micro-organisms. This research was necessitated by the need to counter the rapid emergence of drug-resistant human pathogens, which is becoming a regular phenomenon in the world making life-threatening illnesses easily curable infections. The study was done to determine the antimicrobial activities by agar diffusion inhibition assay of black ...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of astragaloside IV on cognitive dysfunction in rats with cerebrally infarcted via TGF- β / Smad signaling pathway.
Effect of astragaloside IV on cognitive dysfunction in rats with cerebrally infarcted via TGF-β / Smad signaling pathway. Cell Mol Biol (Noisy-le-grand). 2020 May 15;66(2):87-92 Authors: Li L, Guo Y, Zhao Y, Wei J, Jin Y Abstract Cerebral infarction is an acute cerebrovascular disease caused by abnormal blood circulation in the brain. In the present study, we investigate the effect of astragaloside IV on cognitive dysfunction in cerebrally infarcted rats via transforming growth factor-β (TGF-β) / Smad signaling pathway. For this purpose, 45 rats were divided into three groups including a...
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of dexmedetomidine pretreatment on rats with sepsis-induced acute kidney injury and miR-146a expression.
In conclusion, DEX can improve oxidative stress and inflammatory responses in rats with SAKI, reduce the severity of the renal injury, and up-regulate the expression level of miR-146a. PMID: 32415933 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comprehensive hematological reference intervals in a healthy adult male population.
This study established RIs for 12 routinely used hematological parameters in a healthy male population living in the northeastern region of Iran. Established RIs differed from those previously reported by other cohorts, highlighting the importance of population-specific RIs. PMID: 32415934 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research