Flavonoids, bioactive components of propolis, exhibit cytotoxic activity and induce cell cycle arrest and apoptosis in human breast cancer cells MDA-MB-231 and MCF-7 - a comparative study.
Authors: Kabała-Dzik A, Rzepecka-Stojko A, Kubina R, Iriti M, Wojtyczka RD, Buszman E, Stojko J Abstract Breast cancer is one of the most common causes of mortality in women. Flavonoids, among other compounds, are bioactive constituents of propolis. In this comparative study, we investigated the effects of flavonoids apigenin (API), genistein (GEN), hesperidin (HES), naringin (NAR) and quercetin (QUE) on the proliferation, apoptosis, and cell cycle of two different human cancer cells - MDA-MB-231, estrogen-negative, and MCF-7, estrogen-positive receptor breast carcinoma cells. Many cytotoxic reports of flavonoids ...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antiviral activity of Veronica persica Poir. on herpes virus infection.
Authors: Sharifi-Rad J, Iriti M, Setzer WN, Sharifi-Rad M, Roointan A, Salehi B Abstract The lack of an effective anti-viral agent and the emergence of drug-resistant strains dictate a real need for discovery of novel therapies able to ameliorate viral infections. In this regards, medicinal plants and natural products offer safe and inexpensive platforms for discovery of efficient and novel anti-viral agents. We have investigated the potential anti-viral activities of Veronica persica Poir.  as a medicinal plant against herpes simplex viruses (HSVs). In vitro screening of the ethanol plant extract against HSV-...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pulicaria vulgaris Gaertn. essential oil: an alternative or complementary treatment for Leishmaniasis.
The objectives of this study were to obtain the essential oil (EO) from Pulicaria vulgaris Gaertn. growing in Iran and to carry out in-vitro antileishmanial screening of the EO against promastigotes of Leishmania major and Leishmania infantum. The EO from the aerial parts of P. vulgaris was extracted by hydrodistillation. Serial dilutions of the EO were screened for in-vitro antileishmanial activity using 96-well microtiter plates. The P. vulgaris EO was active against the promastigote forms of L. major and L. infantum, with IC50 values of 244.70 and 233.65 µg/mL, respectively. Pulicaria vulgaris EO may serve as an a...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pullulan gum production from low-quality fig syrup using Aureobasidium pullulans.
In conclusion, fig syrup was an effective substrate for pullulan production by Aureobasidium pullulans, and, therefore, this byproduct deserves attention for the cost-effective and environmentally friendly pullulan production. PMID: 29981680 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Satyrium nepalense, a high altitude medicinal orchid of Indian Himalayan region: chemical profile and biological activities of tuber extracts.
Authors: Mishra AP, Saklani S, Salehi B, Parcha V, Sharifi-Rad M, Milella L, Iriti M, Sharifi-Rad J, Srivastava M Abstract The present study investigated antioxidant and antibacterial activities of 5 different extracts and derived fractions from the S. nepalense tubers. Identification of the most active fractions, their phytochemical characterization, total phenolic and flavonoid contents, and biological activities were also evaluated. Petroleum ether, chloroform, ethyl acetate, methanol, water extracts and methanol fractions were screened for their antibacterial activity at 10, 50 and 100 mg/mL doses against ten G...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Susceptibility of Leishmania major to Veronica persica Poir. extracts - In vitro and in vivo assays.
In this study, in vitro and in vivo susceptibility of L. major to Veronica persica Poir. extract, a medicinal plant with many applications, has been evaluated. Antileishmanial activity of plant extract was investigated both on cultured L. major promastigotes and in mice challenged with L. major. Animals were divided into three groups including control (without any treatment), test (treated with plant extract) and glucantime (the reference drug) treated groups. After treatments, skin lesion sizes and body weights of animals were checked during 4 weeks. The potential of the plant extract in decreasing the number of parasites...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Veronica persica Poir. extract - antibacterial, antifungal and scolicidal activities, and inhibitory potential on acetylcholinesterase, tyrosinase, lipoxygenase and xanthine oxidase.
In this study, several analyses were done to discover the antimicrobial and scolicidal activities and acetyl cholinesterase (AChE), tyrosinase (TYR), lipoxygenase (LOX), and xanthine oxidase (XO) inhibitory activities of V. persica extract. The results presented that B. subtilis was the most susceptible to the extract (MIC = 40.3 μg/mL), while P. aeruginosa was the most resistant strain (MIC = 250.9 μg/mL) among all bacteria evaluated. The extracts demonstrated significant activity versus E. granulosus (P < 0.5) with dose-dependent inhibitions of the protoscolices. The analyzed plant extract exhibited a high A...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir.
Authors: Sharifi-Rad J, Sharifi-Rad M, Salehi B, Iriti M, Roointan A, Mnayer D, Soltani-Nejad A, Afshari A Abstract With the appearance of new disorders along with inability of some conventional therapies for the treatment of diseases without any side effects, the discovery of safe and efficient therapeutic agents is of utmost importance in the medical area. In this context, medicinal plants as promising therapeutic candidates can provide a reliable and efficient profile. Since free radicals are at the center of various disorder pathways, reducing their production or complete removal of these chemical species could...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antibacterial potential of Saussurea obvallata petroleum ether extract: A spiritually revered medicinal plant.
Authors: Mishra AP, Saklani S, Sharifi-Rad M, Iriti M, Salehi B, Maurya VK, Rauf A, Milella L, Rajabi S, Baghalpour N, Sharifi-Rad J Abstract Uttarakhand Himalayan region holds Asteraceae or Compositae as the largest family of flowering, medicinal and aromatic plants. Species belonging to this family rises from low altitude to the alpine region. Among Asteraceae, Saussurea obvallata (DC.) Edgew. is widely used in several indigenous systems of medicine. Flowers, leaves and rhizomes of S. obvallata are used for several traditional, religious, therapeutic and ornamental purposes. Aim of this study was to determine the...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antibacterial activity of some Lamiaceae species against Staphylococcus aureus in yoghurt-based drink (Doogh).
This study investigated the antibacterial activity of essential oils (EOs) from Thymus vulgaris L., Mentha piperita L. and Ziziphora tenuior L., alone or in combination, against Staphylococcus aureus in industrial doogh. A three-level and three-variable face centered central composite design experiment was used. Results showed that EOs significantly inhibited S. aureus growth after 1 and 7 days of storage. According to the model, the maximum inhibition was obtained in the presence of 0.2% of EO, independently of the type, and no synergistic or additive effects were observed. Slightly lower S. aureus survivals were observed...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-inflammatory and wound healing effects of an essential oils-based bioadhesive gel after oral mucosa biopsies: preliminary results.
Authors: Scotti F, Decani S, Sardella A, Iriti M, Varoni EM, Lodi G Abstract Post-operative management of patients receiving oral biopsy includes the control of edema, pain, infection, and re-epithelization at the surgical site. This clinical study investigates the topical use of a bioadhesive gel, containing essential oils, to promote wound healing and prevent post-operative pain and infection, avoiding the need for surgical suture and chlorhexidine applications. Ten patients, who needed to receive oral biopsies (≤6 mm in diameter) for the diagnosis of mucosal oral lesions, were enrolled. The bioadhesive gel su...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioactive compounds and health benefits of edible Rumex species-A review.
Authors: Prakash Mishra A, Sharifi-Rad M, Shariati MA, Mabkhot YN, Al-Showiman SS, Rauf A, Salehi B, Župunski M, Sharifi-Rad M, Gusain P, Sharifi-Rad J, Suleria HAR, Iriti M Abstract Medicinal and food plants as well as their bioactive fractions have been used by diverse human cultures since ancient times. These plants provide multiple health benefits because of the presence of a plethora of phytochemicals including phenylpropanoids, isoprenoids, alkaloids, sulphated compounds, peptides and polysaccharides that are responsible for various biological activities such as anticancer, antioxidant, antifungal, antibacte...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Rice allelopathy in weed management - An integrated approach.
Authors: Patni B, Chandra H, Mishra AP, Guru SK, Vitalini S, Iriti M Abstract The intensive use of pesticides with low biodegradability and high persistence in soil, surface and ground waters, represents a considerable environmental risk, especially under high weed pressure conditions. Furthermore, the number of herbicide-resistant weeds is increasing. Against this background, the investigation of alternative weed control strategies has taken on considerable importance. Among these, allelopathy as a negative effect of one plant on another due to the direct or indirect (including microorganisms) release of chemicals...
Source: Cellular and Molecular Biology - July 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Epirubicin-calf thymus DNA interaction: a comprehensive investigation using molecular docking, spectroscopy and fluorescent quantum dots.
Authors: Hemati Azandaryani A, Barati A, Shahlaei M, Shamsipur M, Moradi S, Arkan E Abstract Reviewing the mode of interaction between this kind of active pharmaceutical ingredients and DNA has received much more attention in current years. Anthracycline drugs such as Epirubicin are frequently used in cancer treatment for breast cancer treatment. In the present study, the Epirubicin -calf thymus DNA interaction was investigated by using spectroscopic, fluorimetric and molecular docking methods. Water-soluble quantum dots (QDs) with nanometric particle size fabricated and characterized by transmission electron micro...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cytotoxic effect of 2, 5-dimethyl-celecoxib as a structural analog of celecoxib on human colorectal cancer (HT-29) cell line.
In this study, we have investigated the effect of CXB and DMC on the human HT-29 cell line. The cellular viability, caspase-3 activity, and VEGF, NF-κB, and COX-2 genes expressions were assessed respectively with MTT, colorimetric, and real-time RT-PCR methods. DMC, a close analogue of CXB, was more potent in inhibiting the growth of cells (IC50: 23.45 µM at 24 hr) than CXB (IC50: 30.41 µM at 24 hr). Both CXB and DMC caused a significant difference in caspase-3 activity compared to the control group. DMC significantly decreased the NF-κB expression. Down-regulation of the COX-2 mRNA expression in th...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antioxidant capacity and phylogenetic analysis of twenty native grape cultivars in Siirt province, Turkey.
Authors: Fidan M, Erez ME, İnal B, Pinar SM, Altintaş S Abstract The quality of grape cultivars not only depends on the grape cultivar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultiva...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Purification and properties of Manganese Superoxide Dismutase (MnSOD) from Tamarix aphylla L.
Authors: Hamzah NA, Raheif ES Abstract Superoxide dismutase (SOD) of the Tamarix aphylla leaves were detected at optimum conditions that collected in April, May and June. Results indicated the specific activity in the crude extract reaching to 36.76 unit/ mg protein. Crude SOD was purified by several techniques, precipitation with ammonium sulfate (50-75) %, Ion exchange chromatography using DEAE-cellulose and two steps of size exclusion chromatography on sephacryl S-200 column. The obtained specific activity (310 unit/mg protein) and purification fold 7.91. The purified enzyme revealed one band by SDS-polyacrylami...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ROS related enzyme levels and its association to molecular signaling pathway in the development of head and neck cancer.
Authors: Iplik ES, Ertugrul B, Candan G, Pamuk S, Aydemir L, Celik M, Ulusan M, Ergen A, Cakmakoglu B Abstract Given the prevalence and annual incidence of cancer, head and neck cancer is affecting more than 600,000 people each year. In this research, it was decided to investigate that which genes are involved and how MPO, NQO1, SOD2 enzyme levels effective to develop of head and neck cancer and for the first time at the tissue level. 35 tumor tissues in all head and neck anatomy and their surrounding tissue (70 in total) were enclosed the research that received surgery. Determination of the apoptosis genes express...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of Cichorium intybus on serum oxidative stress, liver and kidney volume, and cyclin B1 and Bcl-2 levels in the brains of rats with ethanol induced damage.
Authors: Ergul Erkec O, Arihan O, Colcimen N, Kara M, Karatas E, Demir H, Cetin Ragbetli M Abstract We investigated the effects of an aqueous root extract of Cichorium intybus on Bcl-2 and cyclin B1 levels in the brain, kidney and liver volumes and changes of serum total antioxidant status (TAS) and total oxidant status (TOS) levels in ethanol induced damage in rats. The rats were divided into five groups: non-treated controls (C), maltodextrin in tap water treated (MD), 6.4% ethanol in tap water treated (ET), Cichorium intybus + maltodextrin in tap water treated (CI+MD), and Cichorium intybus + 6.4% ethanol in tap...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

GSK2193874 treatment at heatstroke onset reduced cell apoptosis in heatstroke mice.
Authors: Zhu YH, Pei ZM Abstract Heatstroke is still a potentially fatal threat during summer heat waves, despite improved prevention and treatment. It is reported that the transient receptor potential vanilloid 4 (TRPV4) inhibitor may protect septicemia mice. Many aspects of heatstroke have been defined, from the sepsis-mimic inflammatory response to hyperthermia. Hence, TRPV4 may be a therapeutic target for heatstroke. The results in murine models of heatstroke verified that GSK2193874, as a selected TRPV4 inhibitor, was injected at heatstroke onset, and then reduced the reduction of core temperature, the death ...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cyclic stretch induces VEGFA alternative splicing via Serine/Arginine-Rich Splicing Factor 1.
Authors: Xie ZW, Chen YX, Long CF, Qi YX, Yao QP Abstract Abnormal proliferation of vascular smooth muscle cells (VSMCs) induced by high cyclic stretch is crucial in the vascular remodeling during hypertension. Vascular endothelial growth factor A (VEGFA) alternative splicing plays important roles in the pathological process of vascular diseases and remodeling. However, the roles of VEGFA isoforms in modulating VSMC functions in response to cyclic stretch remain unclear. We hypothesize that high cyclic stretch may induce VEGFA alternative splicing via Serine/arginine-rich splicing factor 1 (SRSF1) which subsequentl...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Involvement of transforming growth factor beta 1 in the transcriptional regulation of nicotinamide N-methyltransferase in clear cell renal cell carcinoma.
In this study, we focused on molecules that could modulate the trascription of the enzyme nicotinamide N-methyltransferase (NNMT) that is known to be up-regulated in ccRCC. Signal transducer and activator of transcription 3 (STAT3), interleukin 6 (IL-6), hepatocyte nuclear factor 1 beta (HNF-1β) and transforming growth factor beta 1 (TGF-β1) expression levels were determined in tumor and non tumor samples obtained from 30 patients with ccRCC, using Real-Time PCR. Results obtained showed that TGF-β1 is significantly (p<0.05) overexpressed in tumor compared with normal tissue samples of ccRCC patients. ...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Kisspeptin increases intracellular calcium concentration by protein kinase C-mediated signaling in the primary cultured neurons from rat hippocampus.
Authors: Serhatlioglu I, Yuksel H, Kacar E, Ozcan S, Canpolat S, Ozcan M, Ayar A, Kelestimur H Abstract In addition to the fact that kisspeptin and its receptor GPR54 are well known to be abundantly expressed in the hypothalamus with suggestive roles in the initiation of puberty and similar reproductive system properties, there is also proof showing that kisspeptin might have influences on hippocampal functions. In our previous study, it was shown that kisspeptin increased free intracellular Ca2+ values ([Ca2+]i) through protein kinase C (PKC) activation in GT1-7 cells. For this reason, we examined the influences o...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioinformatic analysis reveals CYP2C9 as a potential prognostic marker for HCC and liver cancer cell lines suitable for its mechanism study.
In conclusion, this bioinformatic analysis demonstrated that DEGs, such as CYP2C9, might promote the development of HCC, especially in drug metabolism. It could also be used as a new biomarker for diagnosis. PMID: 29974848 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular epidemiology of ESBL-producing Klebsiella pneumoniae isolates in intensive care units of a tertiary care hospital, North of Iran.
Authors: Ghaffarian F, Hedayati M, Sedigh Ebrahim-Saraie H, Atrkar Roushan Z, Mojtahedi A Abstract The emergence of extended-spectrum beta-lactamases (ESBL) producing strains become a great concern, because few antimicrobial agents remain active against them. Due to the lack of data on the genotyping characteristics and antibiotics resistance of clinical isolates of Klebsiella pneumoniae in the north of Iran, this study aimed to determine the occurrence of ESBL-producing isolates and their molecular characteristics in order to analyses their epidemiological relationships. This cross-sectional study performed on 60 ...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sustenance of endothelial cell stability in septic mice through appropriate activation of transient receptor potential vanilloid-4.
This study was carried with a view to unravelling the reason(s) behind these conflicting results. Different doses of the inhibitor were used in the same degree of sepsis, and their effects were determined through assays for sepsis-related physiological indicators such as endothelial injury markers, coagulation index, organ damage indicators, inflammatory factor levels, and cell apoptosis. The results showed that high or low inhibitor levels had no significant effect on sepsis-related physiological indicators. These findings suggest that proper activation of TRPV-4 in sepsis is important for maintaining normal physiological...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pomegranate peel extract inhibits expression of β-catenin, epithelial mesenchymal transition, and metastasis in triple negative breast cancer cells.
Pomegranate peel extract inhibits expression of β-catenin, epithelial mesenchymal transition, and metastasis in triple negative breast cancer cells. Cell Mol Biol (Noisy-le-grand). 2018 May 30;64(7):86-91 Authors: Bagheri M, Fazli M, Saeednia S, Kor A, Ahmadiankia N Abstract The standard treatment for triple-negative breast cancer (TNBC) is chemotherapy, which is highly toxic to patients; thereby, there is a need to identify safer and more effective therapeutic approaches. Medicinal plants constitute a common alternative for cancer treatment. Pomegranate is a well-known fruit in this context, but ...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell dedifferentiation and multiplication of Burdock (Arctium Lappa) as a medicinal plant.
In this study, optimization of tissue culture protocol for A. lappa was carried out as a research technique, as well as technique for further exploitation of this plant. PMID: 29974852 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of HOTAIR rs12826786 and rs1899663 polymorphisms on lung cancer susceptibility and clinicopathological characteristics in a turkish population: a hospital-based case-control study.
In this study, the association between two HOTAIR single nucleotide polymorphisms (SNPs) (rs12826786 and rs1899663) on the risk and clinical characteristics of lung cancer in a Turkish population was investigated. We genotyped HOTAIR rs12826786 and rs1899663 polymorphisms in 180 Turkish people including 87 lung cancer patients (71 males and 16 females) and 93 age-matched healthy controls (67 males and 26 females) by a TaqMan real-time polymerase chain reaction method. The mean age value of the lung cancer patients and control subjects were 59.27 ± 10.55 and 61.77 ± 12.00, respectively. We found that none of t...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitamin D status and oxidative stress in diabetes mellitus.
Authors: Lee WC, Mokhtar SS, Munisamy S, Yahaya S, Rasool AHG Abstract Diabetes mellitus is an epidemic that is gaining global concern. Chronic hyperglycemia in diabetes induces the excess production of free radicals. The deleterious effects of excess free radicals are encountered by endogenous antioxidant defense system. Imbalance between free radicals production and antioxidants defense mechanisms leads to a condition known as "oxidative stress". Diabetes mellitus is associated with augmented oxidative stress that induced micro- and macrovascular complications, which presents a significant risk for card...
Source: Cellular and Molecular Biology - July 7, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Advances in mechanism and treatment strategy of cancer.
Authors: Zeng Y Abstract Cancer is one of the most serious diseases with high incidence and high mortality rate threaten human health and quality of life. The researches on mechanisms of cancer development and metastasis, and effective and reasonable treatment strategies are of great significance. The traditional treatments for tumors such as surgery, radiotherapy, and chemotherapy have some benefits, but many drawbacks such as drug-resistance and side-effects. With the emergence of new therapeutic methods such as tumor-targeted drugs and immune drugs, the survival of cancer patients is improved, but reports on car...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Icariin inhibits autophagy and promotes apoptosis in SKVCR cells through mTOR signal pathway.
In this study, we investigated whether Icariin could induce a reduction of cell proliferation by inhibiting autophagy. SKVCR cells, which are resistant to vincristine, were used for the investigation. We used CCK8 test and flow cytometry assay to study the effects of Icariin on cell proliferation, cell apoptosis and cell circle. We performed transmission electron microscope (TEM), immunohistochemical assay and western blotting assay to study the level of autophagy after Icariin treatment. Finally, we investigated whether the mTOR pathway is a target of Icariin by using mTOR inhibitor rapamycin and detected autophagy and ap...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bacillus Calmette Gu érin (BCG) activates lymphocyte to promote autophagy and apoptosis of gastric cancer MGC-803 cell.
In this study, we investigated the effect of BCG on gastric cancer cell line MGC-803 and studied the potential cooperation of BCG and lymphocyte in determining the final fate of cancer cells. After treatment with BCG, the cell viability was significantly inhibited in a dosage-dependent manner. Flow cytometry assay showed the apoptosis rates were significantly increased by BCG. Using western blot assay, results showed that BCG increased cleaved-caspase-3, LC-3BII and Atg-3. After cocultured with BCG and lymphocyte, the apoptosis rates, the levels of cleaved-caspase-3, and the protein levels of LC-3BII and Atg-3 were signifi...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Luteolin promotes the sensitivity of cisplatin in ovarian cancer by decreasing PRPA1-medicated autophagy.
In this study, luteolin was found to suppress autophagy with reduced expression of LC3-II, but enhanced the inhibition of cell vitality and promoted apoptosis induced by cisplatin, leading to restoration of the sensitivity to cisplatin in ovarian cancer cells through CCK-8, flow cytometry and immunofluorescent assays. Although cisplatin elevated the PARP1 for cell survival, the cisplatin-induced expression of PARP1 was inhibited by luteolin a dose- and time- dependent manner through Q-PCR and WB assays. Further, PARP1 siRNA could further improve the LUT-induced inhibition of cell vitality and restore the sensitivity to cis...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Characterization of imatinib-resistant K562 cell line displaying resistance mechanisms.
Authors: Hekmatshoar Y, Ozkan T, Altinok Gunes B, Bozkurt S, Karadag A, Karabay AZ, Sunguroglu A Abstract Chronic myeloid leukemia (CML) is a hematopoietic malignancy characterized by the t(9; 22) and the related oncogene, BCR-ABL. Tyrosine kinase activity of fusion protein BCR-ABL is the main cause of CML. Even if imatinib is used as a tyrosine kinase inhibitor (TKI) for CML therapy, drug resistance may occur in patients and the clinical failure of imatinib treatment in resistant patients had resulted with the use of another alternative TKIs. BCR-ABL dependent and independent molecular mechanisms have crucial role...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Adenovirus-mediated overexpression FADD induces a significant antitumor effect on human colorectal cancer cells both in vitro and in vivo.
Authors: He X, Peng X, Liu Y, Zhang X, Li H, Yin H Abstract The Wnt/β-catenin signaling pathway plays important roles in cancers such as colorectal cancer. Colon cancer cells secrete and express high levels of β-catenin, which may stimulate autocrine signaling and further enhance activities of the canonical Wnt signaling pathway. Free β-catenin in the cytoplasm and nucleus leads to its association with T cell factor (TCF)/lymphocyte enhancing factor (Lef) transcription factors, and subsequent transcriptional activation of downstream target genes. FADD plays a key role in cellular apoptosis in many di...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of SIRT1 inhibits proliferation and promotes apoptosis of paclitaxel-resistant human cervical cancer cells.
This study investigates the role of Sirtuin 1 (SIRT1) in paclitaxel (PTX)-resistant CC lines. We used qPCR and Western blots to measure SIRT1 mRNA and protein expressions in 10 matched clinical cancer tissues. We compared the expression levels of SIRT1 between sensitive CC cell lines and PTX-resistant cell lines. Subsequently, we used SIRT1 siRNA to knockdown the expression of SIRT1, and then measured cell proliferation, cell apoptosis rate, cell cycle distribution, and expression levels of Bcl-2 and Bax in PTX-sensitive Hela cell line, PTX-resistant Hela and Sila-resistant cell lines. Finally, we detected the location and...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion by targeting CEMIP and CADM3.
In this study, quantitative real-time PCR (qRT-PCR) and Western blot assays were used to determine the expression levels of miR-140-5p, cell migration-inducing protein (CEMIP), and cell adhesion molecule 3 (CADM3) in RB tissues and cell-lines. The proliferation ability was detected by cell-counting kit 8 (CCK-8), Edu staining, and colony formation assay. The cell cycle and migration and invasion abilities were measured by flow cytometry, wound-healing assay and Transwell assays, respectively. The correlation between miR-140-5p and CEMIP/CADM3 were then confirmed by immunofluorescence (IF) and dual-luciferase reporter assay...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Visfatin increases miR-21 to promote migration in HCC.
In this study, we aimed to investigate the serum levels of visfatin and miR-21 in HCC patients, to analysis the relationship between the pathological features and the plasma level of visfatin or miR-21, and to explore the roles of visfatin and miR-21 in migration of HCC cells. Our results showed that the serum levels of visfatin and miR-21 were significant higher in HCC patients than healthy subjects. The diagnostic sensitivity of serum visfatin was 82.5% and the specificity was 65.0%. The serum visfatin was significantly associated with the histology and metastasis. Visfatin induced miR-21 expression and cell migration in...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Influence of the vaccinating density of A549 cells on tumorigenesis and distant organ metastasis in a lung cancer mice model.
In this study, A549 cells were subcutaneously injected into mice at 1 × 107 cells/mL, 5 × 106 cells/mL, and 1 × 106 cells/mL or intravenously at 1 × 106 cells/mL, 5 × 106 cells/mL, and 1 × 106 cells/mL. Then, histology analysis, immunohistochemistry staining, and in-situ TUNEL assay were performed to evaluate tumor growth and metastasis. Results showed that subcutaneously injecting the A549 cells could develop tumors and that fewer apoptotic cells were found in the 5 × 106 cells/mL group than in the other two groups. In groups intravenously injected with A549 cells, there were tumo...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Flavored Guilu Erxian decoction inhibits the injury of human bone marrow mesenchymal stem cells induced by cisplatin.
Authors: Ke B, Shi L, Xu Z, Wu G, Gong Y, Zhu L, Wang Y, Ouyang H, Wang X Abstract To examine the exact role of flavored Guilu Erxian decoction, a Traditional Chinese Medicine (TCM) in the treatment of cisplatin-induced side-effects in bone marrow mesenchymal stem cells (BM-MSCs). BM-MSCs were isolated from bone marrow collected from SD rats and identified by flow cytometry. Cells were cultivated in MEM alpha medium containing 5% (TCM-L), 10% (TCM-M) and 20% (TCM-H) dosages of flavored Guilu Erxian decoction with or without cisplatin. Cell viability was determined through CCK-8 and thymidine analog 5-ethynyl-2'-deo...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The PI3K and AIB1 interaction is involved in estrogen treated breast cancer cells.
Authors: Li Z, Deng X, Wu G, Qiu R, Ju X, Wang Y, Zhang J Abstract AIB1 was involved in the development and progression of breast cancer. Although it was found that AIB1 could be phosphorylated by some kinases including PI3K, the function of AIB1 and AKT interaction in breast cancer is not well defined. MCF-7 cells were transfected with pERE-Luc AKT and/or AIB1 plasmids, and then ERE luciferase activity in presence or absence of estrogen (E2) were measured. Plasmids containing PTEN and an PI3K inhibitor LY294002 were transfected into or treated cells to identify the interaction of PI3K/AKT and activation of AIB1, a...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Levels of serum bilirubin in small cell lung cancer and non-small cell lung cancer patients.
Authors: Jiang D, Shi J, Yuan M, Duan X, Li L, Li Q Abstract Elevated bilirubin has been associated with protection of cardiovascular and kidney systems, whereas decreased bilirubin may predispose respiratory diseases. However, whether serum bilirubin levels are associated with lung cancer remains unclear. Here, clinical and pathologic data of a cohort of 363 lung cancer patients along with 363 age-and gender-matched healthy subjects were collected. The association of serum bilirubin levels with lung cancer was analyzed. The levels of serum bilirubin were significantly lower in lung cancer patients. The aspartate t...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA-200 as potential diagnostic markers for colorectal cancer: meta-analysis and experimental validation.
Authors: Peng Z, Zhu W, Dai J, Ju F Abstract Members of microRNA(miR)-200 family is proposed as promising biomarkers for colorectal cancer (CRC). However, their expression in CRC patients, and whether them could identify as new biomarkers of cancers are inconsistent and controversy. Therefore, a meta-analysis was performed to assess the diagnostic value of miR-200 family members in CRC patients. This meta-analysis screened 6 studies, including 191 patients with colorectal cancer at stage IV, 446 patients with colorectal cancer at stage I~III and 98 normal controls, and performed using bivariate and hierarchical sum...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A meta-analysis of microRNA-17 as a potential biomarker in diagnosis of colorectal cancer.
Authors: Liu T, Yin L, Yan G, Li C, Wang L Abstract This meta-analysis was aimed to determine the diagnostic accuracy of circulating microRNA-17 for colorectal Cancer (CRC). Databases including PubMed, Embase, Web of Science, Cochrane Library and China National Knowledge Infrastructure (CNKI) were searched up to February 23, 2018 for eligible studies. Quality Assessment of Diagnostic Accuracy Studies (QUADAS) was employed to assess the quality of the included studies. Meta-analysis was performed in STATA 13.0. Ten studies with total 938 CRC patients and 638 control individuals were included in this meta-analysis. A...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Levels of peripheral Th17 cells and serum Th17-related cytokines in patients with colorectal cancer: a meta-analysis.
This study sought to quantitatively summarize the clinical cytokine data. Multiple reports have described the proportion of Th17 cells in peripheral blood and serum levels of Th17-related cytokines in patients with colorectal cancer (CRC). To clarify the status of Th17 cells and Th17-related cytokines in CRC patients, we did a meta-analysis of the results published previously to quantitatively assess the levels of peripheral Th17 cells and serum Th17-related cytokines in patients with CRC. We searched PubMed, Embase, web of Science, Cochrane Library and China National Knowledge Infrastructure (CNKI) systematically for stud...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The regulatory effect of Xiaoyao San on glucocorticoid receptors under the condition of chronic stress.
Authors: Lu J, Fu L, Qin G, Shi P, Fu W Abstract In modern society, fierce competitions cause yearly increase of depression and anxiety. Xiaoyao San is a traditional Chinese medicine which relieves depression and nourishes liver. The active ingredients contain saikoside A, saikoside C, saikoside D, ferulic acid, ligustilide, Atractylenolide I, Atractylenolide II, Atractylenolide Ⅲ, paeoniflorin, Albiflorin, liquiritin, glycyrrhizic acid and pachymic acid. In stress condition, glucocorticoid receptors participate in the hypothalamus-pituitarium-adrenal gland (HPA) axis to regulate the balance of organism. In respo...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Huge abdominal and perineal aggressive angiomyxoma: A misdiagnosed case report and literature review.
Authors: Ma J, Wan J, Jiang B, Wang H, Zhang W, Zhang X Abstract Aggressive angiomyxoma (AA) is a distinctive soft tissue tumor with a high risk of local recurrence. Clinicians must be aware of this rare tumor pre-operatively. Excision is the preferred method of AA treatment. The case report presents a case of a 36-year-old woman who was difficulty in walking due to a non-painful tumor in the abdomen and perineum. She was misdiagnosed as abdomen neurofibroma for more than 10 years, and an operation was performed in 1997. However, the tumor was incompletely resected because its huge volume accompanies with extensive...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of serum levels of IL-6, IL-8, TGF- β and TNF-α in coronary artery diseases, stable angina and participants with normal coronary artery.
Comparison of serum levels of IL-6, IL-8, TGF-β and TNF-α in coronary artery diseases, stable angina and participants with normal coronary artery. Cell Mol Biol (Noisy-le-grand). 2018 Apr 30;64(5):1-6 Authors: Sepehri ZS, Masoomi M, Ruzbehi F, Kiani Z, Nasiri AA, Kohan F, Sheikh Fathollahi M, Kazemi Arababadi M, Kennedy D, Asadikaram GA Abstract Cytokines, which typically regulate the immune responses, play a role in cardiovascular diseases such as coronary artery diseases (CAD) and ischemic heart diseases (IHD). The aims of this study were to evaluate serum levels of IL-6, IL-8, TGF-β ...
Source: Cellular and Molecular Biology - May 8, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Immunoexpression of progesterone receptor, epithelial growth factor receptor and galectin-3 in uterine smooth muscle tumors.
Authors: Soltan MM, Albasry AM, Eldosouky MK, Abdelhamid HS Abstract Uterine smooth muscle tumors constitute a spectrum of neoplasms. Diagnosis of leiomyomas (LMs) is usually straight forwards; however, atypical leiomyomas (ALMs) and smooth muscle tumors of uncertain malignant potential (STUMPs) have overlapping features and need to be distinguished from leiomyosarcoma. To evaluate progesterone receptor (PR), epithelial growth factor receptor (EGF-R), and galectin-3 expression in LMs, ALMs, STUMPs, and leiomyosarcomas and to assess their possible role in differentiating those tumors. Immunoexpression of EGF-R, PR, ...
Source: Cellular and Molecular Biology - May 8, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research