Association of the SOD2 (rs2758339 and rs5746136) polymorphisms with the risk of heroin dependency and the SOD2 expression levels.
CONCLUSION: The rs2758339 and rs5746136 polymorphisms of the SOD2 are associated with the risk of HD but not associated with the SOD2 expression level. PMID: 29459008 [PubMed - in process] (Source: Gene)
Source: Gene - February 22, 2018 Category: Genetics & Stem Cells Authors: Boroumand F, Mahmoudinasab H, Saadat M Tags: Gene Source Type: research
Characterization of metabolic network of oxalic acid biosynthesis through RNA seq data analysis of developing spikes of finger millet (Eleusine coracana): Deciphering the role of key genes involved in oxalate formation in relation to grain calcium accumulation.
Abstract In the present study, we identified seven major genes of oxalic acid biosynthesis pathway (SGAT, GGAT, ICL, GLO, MHAR, APO and OXO) from developing spike transcriptome of finger millet using rice as a reference. Sequence alignment of identified genes showed high similarity with their respective homolog in rice except for OXO and GLO. Transcript abundance (FPKM) reflects the higher accumulation of identified genes in GP-1 (low calcium genotype) as compared to GP-45 (high calcium genotype) which was further confirmed by qRT-PCR analysis, indicating differential oxalate formation in both genotypes. Determina...
Source: Gene - February 22, 2018 Category: Genetics & Stem Cells Authors: Akbar N, Gupta S, Tiwari A, Singh KP, Kumar A Tags: Gene Source Type: research
Plasma microRNA signature of patients with IgA nephropathy.
Abstract We looked for differentially expressed MicroRNAs (miRNAs) in Immunoglobulin A nephropathy (IgAN). Forty-eight miRNAs were identified through the initial screening phase (2 IgAN pools vs. 1 normal control (NC) pool) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel (miRCURY-Ready-to-Use-PCR-Human-panel-I + II-V1.M). By qRT-PCR, these miRNAs were further assessed in the training (32 IgAN VS. 31 NCs) and testing stages (51 IgAN VS. 51 NCs). The renal pathological lesions of patients with IgAN were evaluated according to Lee's grading system. We discovered a p...
Source: Gene - February 22, 2018 Category: Genetics & Stem Cells Authors: Wu J, Zhang H, Wang W, Zhu M, Qi LW, Wang T, Cheng W, Zhu J, Shan X, Huang Z, Zhang L, Chen Y, Sun B, Zhao X, Qian J, Zhu W, Zhou X, Xing C Tags: Gene Source Type: research
Dyslipidemia, steatohepatitis and atherogenesis in lipodystrophic apoE deficient mice with Seipin deletion.
Abstract SEIPIN is an integral membrane protein located in the endoplasmic reticulum, regulating adipocytes differentiation and lipolysis. Deficiency of Seipin in mice causes severe general lipodystrophy, accompanied by insulin resistance, postprandial hypertriglyceridemia and steatohepatitis. In atherosclerosis-prone Ldlr null (Ldlr-/-) mice, lipodystrophy caused by Seipin deletion even led to severe hypercholesteremia and accelerated atherogenesis, when challenged with an atherogenic diet. However, whether the phenotypes observed in Seipin-/-Ldlr-/- mice were a common consequence due to lipodystrophy, rather tha...
Source: Gene - February 13, 2018 Category: Genetics & Stem Cells Authors: Liao J, Liu X, Gao M, Wang M, Wang Y, Wang F, Huang W, Liu G Tags: Gene Source Type: research
Role of Forkhead Box O (FOXO) transcription factor in aging and diseases.
Abstract Fork head box O (FOXO) transcription factor is a key player in an evolutionarily conserved pathway. The mammalian FOXO family consists of FOXO1, 3, 4 and 6, are highly similar in their structure, function and regulation. To maintain optimum body function, the organisms have developed complex mechanisms for homeostasis. Importantly, it is well known that when these mechanisms dysregulate it results in the development of age-related disease. FOXO proteins are involved in a diverse cellular function and also have clinical significance including cell cycle arrest, cell differentiation, tumour suppression, DNA...
Source: Gene - February 13, 2018 Category: Genetics & Stem Cells Authors: Tia N, Singh AK, Pandey P, Azad CS, Chaudhary P, Gambhir IS Tags: Gene Source Type: research
Retraction notice to Pigment epithelium derived factor play a positive role in bone mineralization of osteoblasts derived from diabetic patients [GENE 627C (2017) 563 - 568 of retracted article].
PMID: 29325768 [PubMed - in process] (Source: Gene)
Source: Gene - January 13, 2018 Category: Genetics & Stem Cells Authors: Song N, Zhong J, Zhang J, Yu J, Li J, Qi J, Yang J, Qiu Y, Su W, Feng Z, Wang H Tags: Gene Source Type: research
Associations of GBP2 gene copy number variations with growth traits and transcriptional expression in Chinese cattle.
This study was performed to determine the presence of GBP2 CNV (relative to Angus cattle) in 466 individuals representing six main cattle breeds from China, identify its relationship with growth, and explore the biological effects of gene expression. There were two CNV regions in the GBP2 gene, for three types, CNV1 loss type (relative to Angus cattle) was more frequent in XN than other breeds, and CNV2 loss type (relative to Angus cattle) was more frequent in XN and CDM than other breeds. Though the GBP2 gene copy number presented no correlation with the transcriptional expression of JX (P > .05), but the transcrip...
Source: Gene - January 8, 2018 Category: Genetics & Stem Cells Authors: Zhang GM, Zheng L, He H, Song CC, Zhang ZJ, Cao XK, Lei CZ, Lan XY, Qi XL, Chen H, Huang YZ Tags: Gene Source Type: research
Fat mass and obesity-associated gene rs9939609 polymorphism is a potential biomarker of recurrent venous thromboembolism in male but not in female patients.
In conclusion, our results show that FTO rs9939609 polymorphism in recurrent VTE may differ according to gender and FTO polymorphism may predict VTE recurrence in male patients. PMID: 29325734 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 8, 2018 Category: Genetics & Stem Cells Authors: Ahmad A, Memon AA, Sundquist J, Svensson PJ, Zöller B, Sundquist K Tags: Gene Source Type: research
Whole transcriptome analysis reveals potential novel mechanisms of low-level linezolid resistance in Enterococcus faecalis.
Abstract Linezolid is an oxazolidinone antibiotic commonly used to treat serious infections caused by vancomycin-resistant enterococcus. Recently, low-level linezolid resistant Enterococcus faecalis strains have emerged worldwide, but the resistant mechanisms remain undefined. Whole-transcriptome profiling was performed on an E. faecalis strain P10748 with low-level linezolid resistance in comparison with a linezolid-susceptible strain 3138 and the standard control strain ATCC29212. The functions of differentially expressed genes (DEGs) were predicted, with some DEGs potentially involved in drug resistance were va...
Source: Gene - January 8, 2018 Category: Genetics & Stem Cells Authors: Hua R, Xia Y, Wu W, Yan J, Yang M Tags: Gene Source Type: research
Differential association of DENND1Agenetic variants with polycystic ovary syndrome in Tunisian but not Bahraini Arab women.
CONCLUSION: The association of DENND1A rs10818854 and rs10986105 variants with PCOS in Tunisian but not Bahraini women confirms the dependence of this association on the genetic/racial origin of study subjects. PMID: 29325736 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 8, 2018 Category: Genetics & Stem Cells Authors: Dallel M, Sarray S, Douma Z, Hachani F, Al-Ansari AK, Letaifa DB, Mahjoub T, Almawi WY Tags: Gene Source Type: research
Expression of hNeuritn protein in a baculovirus expression system and the analysis of its activity.
Abstract Neuritin plays an important role in the development and regeneration of the nervous system, and shows good prospects in the treatment and protection of the nervous system. To characterize neuritin function, we constructed a baculovirus expression system of neuritin, and identified the biological activity of the neuritin protein. The results and showed that the expression product could promote the neurite growth of dorsal root ganglion in chicken embryos. The neuritin open reading frame was amplified and cloned into the plasmid pFastBac™HTA. The pFastBac™HTA-neuritin was confirmed to be correct...
Source: Gene - January 7, 2018 Category: Genetics & Stem Cells Authors: Zhang S, Huang Y, Zhu J, Shan L, Gao J, Zhang Y, Yu N, Yang L, Huang J Tags: Gene Source Type: research
Polymerase epsilon mutations and concomitant β2-microglobulin mutations in cancer.
Polymerase epsilon mutations and concomitant β2-microglobulin mutations in cancer. Gene. 2018 Jan 07;: Authors: Voutsadakis IA Abstract Mutations in the exonuclease domain of polymerase epsilon (POLE), an enzyme of DNA synthesis, are involved in a newly described syndrome of colorectal polyposis and cancer, and have been associated with a high mutation burden with or without microsatellite instability (MSI) phenotype. The exonuclease domain of POLE executes a proofreading function that decreases the mutation rate during DNA replication by an estimated of one to two orders. The high mutation burde...
Source: Gene - January 7, 2018 Category: Genetics & Stem Cells Authors: Voutsadakis IA Tags: Gene Source Type: research
Identification of key microRNAs affecting drip loss in porcine longissimus dorsi by RNA-Seq.
Abstract MicroRNAs (miRNAs) are a class of ~22-nt non-coding small RNA that play an important role in various metabolic processes, mainly through suppressing the expression of protein coding genes at post-transcriptional level. Drip loss (DL) is one of the most important meat quality traits affecting the end product yield and quality of pork. To date, the underlying regulatory factors involved in DL trait are still incompletely understood. In the present study, we constructed two small RNA libraries with longissimus dorsi muscles from the higher (WJJ-H group) and the lower DL (WJJ-L group) individuals, and applied...
Source: Gene - January 7, 2018 Category: Genetics & Stem Cells Authors: Wei W, Li B, Liu K, Jiang A, Dong C, Jia C, Chen J, Liu H, Wu W Tags: Gene Source Type: research
Association study of miR-100, miR-124-1, miR-218-2, miR-301b, miR-605, and miR-4293 polymorphisms and the risk of breast cancer in a sample of Iranian population.
Abstract MicroRNAs (miRNAs) regulate genes expression by directly binding to the 3' untranslated region (3'UTR) of specific target mRNAs. Single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) are proposed to be important in the development of breast cancer (BC). In the present study, we conducted a case-control study with 266 BCE patients and 288 control women to examine the possible association of miRNAs polymorphisms (miR-100 rs1834306, miR-124-1 rs531564, miR-218-2 rs11134527, miR-301b rs384262, miR-605 rs2043556, and miR-4293 rs12220909) with BC susceptibility. Genotyping was done by polymerase chain ...
Source: Gene - January 6, 2018 Category: Genetics & Stem Cells Authors: Danesh H, Hashemi M, Bizhani F, Hashemi SM, Bahari G Tags: Gene Source Type: research
The molecular mechanisms of action of the endocrine disrupting chemical bisphenol A in the development of cancer.
Abstract The endocrine disrupting chemical (EDC) is an exogenous substance or mixture that alters the function of the endocrine system and consequently causes adverse effects in intact organisms. Bisphenol A (BPA), one of the most common endocrine disrupting chemicals is a carbon-based synthetic compound used in the production of water bottles, cans, and teeth suture materials. It is known to be a xenoestrogen as it interacts with estrogen receptors and acts as agonist or antagonist via estrogen receptor-dependent signaling pathways. BPA has been associated with serious health effects in humans and wildlife. It el...
Source: Gene - January 6, 2018 Category: Genetics & Stem Cells Authors: Shafei AE, Ramzy MM, Hegazy AI, Husseny AK, El-Hadary UG, Taha MM, Mosa AA Tags: Gene Source Type: research
Molecular cloning, characterization, and expression analysis of the three cysteine and glycine-rich protein genes in the Chinese fire-bellied newt Cynops orientalis.
In this study, we cloned the complete open reading frame (ORF) sequences of the three CSRP genes from the Chinese fire-bellied newt, Cynops orientalis (C. orientalis). The complete ORF sequences of Co-CSRP1, Co-CSRP2, and Co-CSRP3 were 582, 582, and 576bp, respectively, and encoded 193, 193, and 191 amino acids, respectively. The deduced amino acid sequences of the three CRP members showed high similarities with that of other species, particularly, with amphibians. Co-CSRP1 was highly expressed in the kidney, limb, and stomach, however, the expression was low in the spleen, heart, intestine, liver, and tail (P
Source: Gene - January 6, 2018 Category: Genetics & Stem Cells Authors: Feng Y, Feng J, Zheng H, Wang W, Chen F, Yu Y, Cui J Tags: Gene Source Type: research
Differential distribution and association of FTO rs9939609 gene polymorphism with obesity: A cross-sectional study among two tribal populations of India with East-Asian ancestry.
This study revealed the FTO rs9939609 polymorphism is observed to be associated with obesity only among the Liangmai tribe not among the Mizo tribe. The differential distribution and association observed in the two selected tribes, inhabited in a similar geographical region, could be attributed to differences in their migratory histories in terms of both route and time of settlement. PMID: 29317321 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 6, 2018 Category: Genetics & Stem Cells Authors: Ningombam SS, Chhungi V, Newmei MK, Rajkumari S, Devi NK, Mondal PR, Saraswathy KN Tags: Gene Source Type: research
MiR-93-5p up-regulation is involved in non-small cell lung cancer cells proliferation and migration and poor prognosis.
CONCLUSION: MiR-93-5p is up-regulated in NSCLC and plays an oncogenic role by inhibiting PTEN and RB1, suggesting miR-93-5p may be a novel prognostic indicator and a therapeutic target in NSCLC. PMID: 29309884 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Yang W, Bai J, Liu D, Wang S, Zhao N, Che R, Zhang H Tags: Gene Source Type: research
HBXIP regulates etoposide-induced cell cycle checkpoints and apoptosis in MCF-7 human breast carcinoma cells.
In this study, we investigate the effects of HBXIP gene silencing on etoposide chemosensitivity in MCF-7 human breast cancer cells. We find that etoposide increases HBXIP expression and promotes mobilization of HBXIP to the nucleus in MCF-7 cells. Knockdown of HBXIP alleviates etoposide-induced G2/M or S phase arrest. Upregulation of p53 and p21 upon etoposide treatment is attenuated in HBXIP knock-down cells. Moreover, HBXIP gene silencing sensitizes etoposide-induced cell apoptosis and cleavage of caspase-9 and PARP in MCF-7 cells. Knockdown of HBXIP expression by RNAi abrogates the etoposide-activated ERK and Akt. These...
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Fei HR, Li ZJ, Ying-Zhang, Yue-Liu, Wang FZ Tags: Gene Source Type: research
The association of polymorphic variants, rs2267788, rs1333049 and rs2383207 with coronary artery disease, its severity and presentation in North Indian population.
CONCLUSION: The polymorphic variants of Apo-A5; rs2266788 (C), 9p21.3; rs1333049 (C) rs2383207 (A) are associated with CAD, its severity and exerts the risk of MI in North Indian population. PMID: 29309886 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Kashyap S, Kumar S, Agarwal V, Misra DP, Rai MK, Kapoor A Tags: Gene Source Type: research
Antileukemic effect of paclitaxel in combination with metformin in HL-60 cell line.
Abstract Acute promyelocytic leukemia (APL) is a subtype of AML that is a mixture of hematological malignancy, characterized by a specific translocation t(15;17). The using of all-trans retinoic acid (ATRA) with arsenic trioxide (ATO) or chemotherapeutic agents or both of these agents, composes main treatment strategy of APL. While it is possible to achieve success in treatment of low-risk APL with current treatment regimens, such success is not mentioned in high-risk APL. So, it may develop new approaches for treatment regimens for high-risk APL. In the present study, we aimed to investigate the effects of combin...
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Asik A, Kayabasi C, Yelken BO, Susluer SY, Sigva ZOD, Okcanoglu TB, Saydam G, Avci CB, Gunduz C Tags: Gene Source Type: research
A key structural gene, AaLDOX, is involved in anthocyanin biosynthesis in all red-fleshed kiwifruit (Actinidia arguta) based on transcriptome analysis.
Abstract Study on kiwifruit (Actinidia chinensis and A. deliciosa) color mainly concentrated in green and yellow-fleshed cultivars, less about molecular mechanism of red-fleshed trait formation, rarely in all-red typed fruit. Using 'Tianyuanhong' and 'Yongfengyihao' ('TY', a kind of all-red typed cultivar, from Actinidia arguta; 'YF', a kind of all-green type cultivar, also from Actinidia arguta) as experimental material, we performed RNA-seq to obtain 202,742 unigenes with an average length of 603bp and N50 of 873bp via transcriptome data analysis. Of these unigenes, 72,508 (35.76%) were annotated and 997 were as...
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Li Y, Fang J, Qi X, Lin M, Zhong Y, Sun L Tags: Gene Source Type: research
Matrix metalloproteinase and tissue inhibitors of metalloproteinases gene polymorphisms in disorders that influence fertility and pregnancy complications: A systematic review and meta-analysis.
eza N Abstract Matrix metalloproteinase (MMP) and tissue inhibitors of metalloproteinase (TIMP) gene polymorphisms have been extensively evaluated as predisposing factors to human reproductive disorders. However, the evidence available is inconsistent. Therefore, we performed a systematic review and meta-analysis to provide the first comprehensive synopsis of case-control studies that investigated the association of MMP and TIMP gene polymorphisms with disorders that influence fertility and pregnancy complications. Literature search was performed using PubMed and Scopus databases. We included 42 case-control studi...
Source: Gene - January 5, 2018 Category: Genetics & Stem Cells Authors: Barišić A, Pavlić SD, Ostojić S, Pereza N Tags: Gene Source Type: research
The complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) with phylogenetic implications for freshwater crabs.
Abstract In the present study, we determined the complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) for the first time. The genome is 15,380bp in length and typically consists of 37 genes. When the gene order was compared to the ancestral crustacean type, two tRNA genes (tRNAHis and tRNAGln) were rearranged in H. lichuanense, and the translocation of tRNAGln appeared only in Potamoidea crabs, such as Geothelphusa dehaani and Sinopotamon xiushuiense, supporting the monophyly of the Potamoidea superfamily. Thirteen protein-coding genes and 2 rRNA genes were divided into five complexes t...
Source: Gene - January 4, 2018 Category: Genetics & Stem Cells Authors: Bai J, Xu S, Nie Z, Wang Y, Zhu C, Wang Y, Min W, Cai Y, Zou J, Zhou X Tags: Gene Source Type: research
Global gene expression analysis of knockdown Triosephosphate isomerase (TPI) gene in human gastric cancer cell line MGC-803.
In conclusion, DEGs and hub genes identified in the present study help us understand the molecular mechanisms of TPI in the carcinogenesis and progression of gastric cancer. PMID: 29307852 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 4, 2018 Category: Genetics & Stem Cells Authors: Ouyang P, Lin B, Du J, Pan H, Yu H, He R, Huang Z Tags: Gene Source Type: research
Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice.
Abstract The core circadian clock gene, Clock, is a positive component of the transcription/translation feedback loop in the master pacemaker suprachiasmatic nucleus (SCN) in mammals. The robust daytime peak of some clock genes in the wild-type SCN is absent in Clock mutant mice. However, very little is known about the impact of Clock mutation on the expression of other functional genes in SCN. Here, we performed cDNA microarray and found 799 differentially expressed genes (DEGs) at zeitgeber time 2 (ZT2) and 1289 DEGs at ZT14 in SCN of Clock△19/△19 mutant mice. KEGG pathway analysis showed that the changed mR...
Source: Gene - January 4, 2018 Category: Genetics & Stem Cells Authors: Wang Y, Lv K, Zhao M, Liang F, Chen H, Ji G, Wang T, Zhang Y, Cao H, Li Y, Qu L Tags: Gene Source Type: research
Evidence of post-transcriptional readthrough regulation in FGF5 gene of alpaca.
Abstract Two different phenotypes are described in alpaca, identified as suri and huacaya, which differ in the type of fleece. The huacaya fleece is characterized by compact, soft and highly crimped fibers, while the suri fleece is longer, straight, less-crimped and lustrous. In our study, the Fibroblast growth factor 5 (FGF5) was investigated as a possible candidate gene for hair length in alpaca (Vicugna pacos). As previously identified in other mammals, our results show that the alpaca FGF5 gene gives rise to a short (FGF5S) and a long (FGF5) isoform. Interestingly, in the long isoform, we observed a point muta...
Source: Gene - January 4, 2018 Category: Genetics & Stem Cells Authors: Pallotti S, Pediconi D, Subramanian D, Molina MG, Antonini M, Morelli MB, Renieri C, La Terza A Tags: Gene Source Type: research
LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli.
CONCLUSION: Our results suggest that LEAs from G. elata play an important role in responses to abiotic stress. PMID: 29305972 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Zeng X, Ling H, Yang J, Li Y, Guo S Tags: Gene Source Type: research
Peroxidasin is regulated by the epithelial-mesenchymal transition master transcription factor Snai1.
Abstract Peroxidasin (PXDN), an ECM protein with peroxidase activity, is integral to basement membrane consolidation through catalysis of sulfilimine bonds in collagen IV. PXDN is also involved in processes where epithelial-to-mesenchymal transition (EMT) takes place, namely fibrosis, development and cancer. We therefore investigated whether PXDN is regulated by the EMT-master-regulator, Snai1. During TGF-β1-induced EMT, PXDN expression decreased by up to 47% in two cervical-carcinoma cell lines, with concomitant increases in Snai1 and vimentin, and decrease in E-cadherin. TGF-β1 induced Snai1 binding to...
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Sitole BN, Mavri-Damelin D Tags: Gene Source Type: research
Circular RNA Atp9b, a competing endogenous RNA, regulates the progression of osteoarthritis by targeting miR-138-5p.
Abstract Osteoarthritis (OA) is the most common joint disease and is mainly characterized by degradation of the articular cartilage. Recently, circular RNAs (circRNAs), novel noncoding RNAs with different biological functions and pathological implications, have been reported to be closely associated with various diseases. Growing evidence indicates that circRNAs act as competing endogenous RNAs (ceRNAs) that bind with microRNAs (miRNAs) and regulate their downstream functions. Here, we identified a new circRNA, circRNA_Atp9b, and further investigated its function in OA using a well-established mouse chondrocyte mo...
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Zhou ZB, Du D, Huang GX, Chen A, Zhu L Tags: Gene Source Type: research
Identification and expression analysis of microRNAs in medaka gonads.
In this study, two small RNA libraries from the ovaries and testes were constructed and sequenced. A total of 285 conserved and 388 novel miRNAs were obtained, among which, 142 mature miRNAs were significantly (> two-fold change) up or down regulated in the testis compared to the ovary. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis showed that miR-430c, miR-26a and miR-202-5p were expressed in a gonad-specific or sex-biased pattern. Fluorescence in situ hybridization (FISH) indicated that miR-202-5p was present throughout spermatogenesis and was only detected at the early stages of ooge...
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Qiu W, Zhu Y, Wu Y, Yuan C, Chen K, Li M Tags: Gene Source Type: research
Functional long non-coding RNAs associated with gastric cancer susceptibility and evaluation of the epidemiological efficacy in a central Chinese population.
CONCLUSION: Our findings showed rs4784659, rs579501 and rs1859168 reduced the susceptibility of gastric cancer. From epidemiological perspective, the lncRNAs with SNPs attenuate the development of gastric cancer. PMID: 29305976 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Duan F, Jiang J, Song C, Wang P, Ye H, Dai L, Zhang J, Wang K Tags: Gene Source Type: research
Functional analysis of a novel ENG variant in a patient with hereditary hemorrhagic telangiectasia (HHT) identifies a new Sp1 binding-site.
Abstract Hereditary Hemorrhagic Telangiectasia (HHT) is a rare disease, with an autosomal dominant inheritance and a worldwide incidence of about 1: 5000 individuals. In>80% of patients, HHT is caused by mutations in either ENG or ACVRL1, which code for ENDOGLIN and Activin A Receptor Type II-Like Kinase 1 (ALK1), belonging to the TGF-β/BMP signalling pathway. Typical HHT clinical features are mucocutaneous telangiectases, arteriovenous malformations, spontaneous and recurrent epistaxis, as well as gastrointestinal bleedings. An additional, but less frequent, clinical manifestation in some HHT patients is ...
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Plumitallo S, Ruiz-Llorente L, Langa C, Morini J, Babini G, Cappelletti D, Scelsi L, Greco A, Danesino C, Bernabeu C, Olivieri C Tags: Gene Source Type: research
Gene expression profiling provides insights into the immune mechanism of Plutella xylostella midgut to microbial infection.
In this study, expressions of the immune-related genes in the midgut of P. xylostella orally infected with Staphylococcus aureus, Escherichia coli and Pichia pastoris were profiled by RNA-seq and qRT-PCR approaches. The results revealed that the Toll, IMD, JNK and JAK-STAT pathways and possibly the prophenoloxidase activation system in P. xylostella could be activated by oral infections, and moricins, gloverins and lysozyme2 might act as important effectors against microorganisms. Subsequent knock-down of IMD showed that this gene was involved in regulating the expression of down-stream genes in the IMD pathway. Our work i...
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Lin J, Xia X, Yu XQ, Shen J, Li Y, Lin H, Tang S, Vasseur L, You M Tags: Gene Source Type: research
Target gene screening and evaluation of prognostic values in non-small cell lung cancers by bioinformatics analysis.
CONCLUSION: Our results provide some potential underlying biomarkers for NSCLC. Further studies are required to elucidate the pathogenesis of NSCLC. PMID: 29305979 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Piao J, Sun J, Yang Y, Jin T, Chen L, Lin Z Tags: Gene Source Type: research
Transcriptomic analysis of topping-induced axillary shoot outgrowth in Nicotiana tabacum.
This study lays a solid foundation for future studies on molecular mechanisms underlying the growth of axillary shoots. PMID: 29292191 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - December 29, 2017 Category: Genetics & Stem Cells Authors: Wang WF, Chen P, Lv J, Chen L, Sun YH Tags: Gene Source Type: research
The functional polymorphisms of ARID5B and IKZF1 are associated with acute myeloid leukemia risk in a Han Chinese population.
In this study, we systematically screened 6 potentially functional SNPs in ARID5B and IKZF1 genes, and conducted a case-control study including 660 AML cases and 1034 cancer-free controls to investigate the associations between these SNPs and AML risk. We found that the variant alleles of rs4509706 and rs11761922 could significantly increase the risk of AML (rs4509706: OR=1.35, 95%CI=1.12-1.62 in additive model; rs11761922: OR=1.29, 95%CI=1.02-1.62 in recessive model). Luciferase reporter assay showed that both rs11761922-G and rs4509706-C significantly increased the luciferase levels as compared with rs11761922-C and rs45...
Source: Gene - December 29, 2017 Category: Genetics & Stem Cells Authors: Cao S, Yang J, Qian X, Jin G, Ma H Tags: Gene Source Type: research
Transcriptome sequencing and comparative analysis of differentially-expressed isoforms in the roots of Halogeton glomeratus under salt stress.
Abstract Although Halogeton glomeratus (H. glomeratus) has been confirmed to have a unique mechanism to regulate Na+ efflux from the cytoplasm and compartmentalize Na+ into leaf vacuoles, little is known about the salt tolerance mechanisms of roots under salinity stress. In the present study, transcripts were sequenced using the BGISEQ-500 sequencing platform (BGI, Wuhan, China). After quality control, approximately 24.08 million clean reads were obtained and the average mapping ratio to the reference gene was 70.00%. When comparing salt-treated samples with the control, a total of 550, 590, 1411 and 2063 DEIs wer...
Source: Gene - December 29, 2017 Category: Genetics & Stem Cells Authors: Yao L, Wang J, Li B, Meng Y, Ma X, Si E, Ren P, Yang K, Shang X, Wang H Tags: Gene Source Type: research
Prevalence of MMP-8 gene polymorphisms in HIV-infected individuals and its association with HIV-associated neurocognitive disorder.
In conclusion, MMP-8 polymorphisms independently and with alcohol and tobacco usage revealed a trend of higher risk for the modulation of HAND severity. MMP-8 -799TT genotype was associated with the advancement of HIV disease. PMID: 29292194 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - December 29, 2017 Category: Genetics & Stem Cells Authors: Singh H, Samani D, Nambiar N, Ghate MV, Gangakhedkar RR Tags: Gene Source Type: research
Molecular cloning and characterization of APOBEC3 family in tree shrew.
Abstract The APOBEC3 family is a series antiviral factors that inhibit the replication of many viruses, such as HIV-1 and HBV. Tree shrews (Tupaia belangeri) possess great potential as an animal model for human diseases and therapeutic responses. However, the APOBEC3 family is unknown in tree shrews. Recent work has showed the presence of the APOBEC3 family in tree shrews. In this work, the cDNA sequences of five APOBEC3 members were identified in tree shrews, namely, tsAPOBEC3A, -3C, -3F, -3G and -3H. The results showed that their sequences encoded a zinc (Z)-coordinating-domain as a characteristic of APOBEC3 pro...
Source: Gene - December 29, 2017 Category: Genetics & Stem Cells Authors: Luo MT, Fan Y, Mu D, Yao YG, Zheng YT Tags: Gene Source Type: research
BmYki is transcribed into four functional splicing isoforms in the silk glands of the silkworm Bombyx mori.
This study provides novel insights into the role of BmYki. The contribution of each BmYki isoform to the modulation of gene expression will be of great interest for further study. PMID: 29289608 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - December 28, 2017 Category: Genetics & Stem Cells Authors: Zeng W, Liu R, Zhang T, Zuo W, Ou Y, Tang Y, Xu H Tags: Gene Source Type: research
Regulatory variants in cancer-related pathway genes predict survival of patients with surgically resected non-small cell lung cancer.
CONCLUSION: Our results suggest that five SNPs in the cancer-related pathway genes may be useful for the prediction of the prognosis in patients with surgically resected NSCLC. PMID: 29289609 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - December 28, 2017 Category: Genetics & Stem Cells Authors: Shin KM, Hong MJ, Lee SY, Jin CC, Baek SA, Lee JH, Choi JE, Kang HG, Lee WK, Seok Y, Lee EB, Jeong JY, Yoo SS, Lee J, Cha SI, Kim CH, Kim YC, Oh IJ, Na KJ, Cho S, Jheon S, Park JY Tags: Gene Source Type: research
Body composition, serum lipid levels, and transcriptomic characterization in the adipose tissue of male pigs in response to sex hormone deficiency.
Abstract It is known that the male hypogonadism plays an important role in regulating adipose metabolism. In the present study, fifteen pairs of full male sibs were divided into a castrated group and an intact group with a paired experiment design. The pigs were slaughtered at an age of 175days. The carcass characteristics and fat deposit of the studied animal were measured, and the hormone and serum lipid levels of the peripheral blood samples were determined, and the differentially expressed genes of the back fat between the two groups were screened with porcine genome array. Our results showed that the absence ...
Source: Gene - December 28, 2017 Category: Genetics & Stem Cells Authors: Yao Y, Ma H, Wu K, Shao Y, Han W, Cai Z, Xu N, Qi M, Zhao C, Wu C Tags: Gene Source Type: research
Variants in GNPTAB, GNPTG and NAGPA genes are associated with stutterers.
Abstract Non-syndromic stuttering is a neurodevelopmental disorder characterized by disruptions in normal flow of speech in the form of repetition, prolongation and involuntary halts. Previously, mutations with more severe effects on GNPTAB and GNPTG have been reported to cause Mucolipidosisll (ML-ll) and Mucolipidosislll (ML-lll), two lysosomal storage disorders with multiple pathologies. We used homozygosity mapping and Sanger sequencing to investigate variants of the three gene in 25 Iranian families with at least two first degree related non-syndromic stutterers. Bioinformatic evaluation and Segregation analys...
Source: Gene - December 28, 2017 Category: Genetics & Stem Cells Authors: Kazemi N, Estiar MA, Fazilaty H, Sakhinia E Tags: Gene Source Type: research
Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis).
In this study, we analyzed the expression patterns of Es-DDX52, Es-DDX6, Es-Vasa and Es-XRN1 both at mRNA and protein levels in different tissues and during gonadal development. It showed that Es-vasa, Es-DDX6 and Es-Xrn1, components of cytoplasmic foci P-bodies, have the similar transcriptional expression pattern, while Es-DDX52 has the reverse tendency. Furthermore, Es-DDX6 and Es-XRN1 proteins have the same localization in testicular tissues. Es-DDX52 mainly distributed in the cytoplasm of spermatogonia, only localized in the nucleus of early and middle spermatid and shifted to pre-acrosome vesicle (later developed into...
Source: Gene - December 27, 2017 Category: Genetics & Stem Cells Authors: Li Q, Yang H, He L, Wang Q Tags: Gene Source Type: research
The rs243866/243865 polymorphisms in MMP-2 gene and the relationship with BP control in obese resistant hypertensive subjects.
no H Abstract We sought to investigate whether the polymorphisms rs243865 (-1306C>T); rs243866 (-1575G>A) and rs2285053 (-735C>T) in metalloproteinases 2 - MMP-2 gene and rs17576 (Q279R), rs17577 (Q668R) and rs3918242 (-1562C>T) in MMP-9 gene are associated with clinical outcomes in obese resistant hypertensive (RH) subjects. One hundred and twenty RH were enrolled in this cross-sectional study and divided into obese (n=63) and non-obese (n=57) according to body mass index. Genotypes were determined by real-time PCR using TaqMan probes. We determined pulse wave velocity (PWV), microalbuminuria and left...
Source: Gene - December 27, 2017 Category: Genetics & Stem Cells Authors: Ritter AMV, de Faria AP, Barbaro NR, Sabbatini AR, Corrêa NB, Brunelli V, Fattoni A, Amorim R, Modolo R, Moreno H Tags: Gene Source Type: research
Sequence analysis of microRNAs during pre-implantation between Meishan and Yorkshire pigs.
Abstract Embryonic implantation in sows is a coordinated interaction between the implantation-competent blastocyst and receptive uterus. In addition, microRNAs are small endogenous non-coding RNAs which are involved in post-transcriptional gene regulation of several biological processes including embryonic implantation. However, the mechanisms of miRNAs involved in embryonic implantation of sows remain largely unknown. Here, we analyzed miRNAome of endometrium on day 9, 12 and 15 of pregnancy and on day 12 of non-pregnancy in Meishan and Yorkshire pigs by Illumina sequencing. From 24 libraries, we identified 312 k...
Source: Gene - December 26, 2017 Category: Genetics & Stem Cells Authors: Li W, Xi Y, Xue S, Wang Y, Wu L, Liu H, Lei M Tags: Gene Source Type: research
The role of DNA methylation in coronary artery disease.
Abstract Epigenetic studies have identified DNA methylation in coronary artery disease (CAD). How the critical genes interact at the cellular level to cause CAD is still unknown. The discovery of DNA methylation inspired researchers to explore relationships in genomic coding and disease phenotype. In the past two decades, there have been many findings regarding the relationship between DNA methylation and CAD development, and the DNA methylation of critical genes have been found to be significantly changed during CAD, including DNA methylation at homocysteine, Alu and long Interspersed Element 1 (LINE-1) repetitiv...
Source: Gene - December 26, 2017 Category: Genetics & Stem Cells Authors: Duan L, Hu J, Xiong X, Liu Y, Wang J Tags: Gene Source Type: research
Downregulation of long noncoding RNA H19 contributes to the proliferation and migration of papillary thyroid carcinoma.
Abstract Recent studies have highlighted important roles for long noncoding RNAs (lncRNAs) during the complex process of carcinogenesis. H19 is an example of an lncRNA that can function either as a tumor promoter or a tumor suppressor. Here, we investigated the role of H19 in papillary thyroid carcinoma (PTC). First, we assessed H19 expression levels in human PTC tissues and PTC cell lines using quantitative real-time PCR. We also established H19-overexpressed PTC cell lines with lentiviral vectors to investigate the effects of H19 on the proliferation and migration of PTC cells. Our results suggest that H19 is do...
Source: Gene - December 26, 2017 Category: Genetics & Stem Cells Authors: Lan X, Sun W, Dong W, Wang Z, Zhang T, He L, Zhang H Tags: Gene Source Type: research
MiR-183 regulates milk fat metabolism via MST1 in goat mammary epithelial cells.
In this study, we analyzed the correlation between differentially expressed miRNAs in goat mammary tissue and the fatty acid composition of goat milk by using Pearson correlations. Results revealed that levels of miR-183 were highly and positively correlated with the fatty acid content in the milk. In addition, we demonstrated that overexpression of miR-183 inhibits milk fat metabolism and inhibition of miR-183 promotes milk fat metabolism. Using Western blot, we demonstrate that MST1, one of the major elements of the Hippo signaling pathway, is a target of miR-183. Immunofluorescence assays revealed that miR-183 targets M...
Source: Gene - December 23, 2017 Category: Genetics & Stem Cells Authors: Chen Z, Shi H, Sun S, Luo J, Zhang W, Hou Y, Loor JJ Tags: Gene Source Type: research