Association between Matrix Metalloproteinase 9 Polymorphisms and Breast Cancer Risk: An Updated Meta-Analysis and Trial Sequential Analysis.
CONCLUSION: Our meta-analysis suggests that MMP-9 rs3918242, but not rs3787268 and rs17576 polymorphisms, may be risk factors for breast cancer. The effect of rs2250889 needs further confirmation with a larger sample size. PMID: 32739585 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 30, 2020 Category: Genetics & Stem Cells Authors: Xu T, Zhang S, Qiu D, Li X, Fan Y Tags: Gene Source Type: research

Genome-wide Identification and Expression Profile of the Sox Gene Family in Different Tissues and During Embryogenesis in the Pacific White Shrimp (Litopenaeus vannamei).
In this study, a total of five members of the sox gene family were identified from the genome of Pacific white shrimp Litopenaeus vannamei and classified into three subgroups based on the conserved HMG-box domain. Among them, three belong to the SoxB subgroup (one in B1 and two in B2), one in the SoxC subgroup, and one in the SoxE subgroup. The five sox genes had different sex-biased expression in some tissues. Sox21, soxB1, and sox14 had a higher expression in ovary than in testis. In comparison, sox4 had a male-biased specific expression in the gonad, hepatopancreas, gill, and eyestalk. There was no difference in soxE ge...
Source: Gene - July 30, 2020 Category: Genetics & Stem Cells Authors: Yao C, Wan H, Zhang Z, Lin J, Wang Y Tags: Gene Source Type: research

HPV11E7 inhibits IMQ-induced chemokine and colony-stimulating factor production in keratinocytes.
Abstract Imiquimod (IMQ) is approved as a first-line treatment for genital warts caused by human papillomavirus (HPV) infection. However, the recurrence rate is very high. HPV E7 protein plays a critical role in HPV immune escape. However, the role of HPV11 E7 protein in genital warts recurrence during IMQ treatment is not clear. Here, we found that the expression profile of NHEK cells was obviously changed after IMQ treatment, and a large number of genes encoding cytokines and genes involved in cytokine-mediated signaling pathways and cellular metabolic signaling pathways were up- or downregulated. HPV11E7 overex...
Source: Gene - July 30, 2020 Category: Genetics & Stem Cells Authors: Zheng H, Zou Z, Wu X, Xu Y, Zhu J, Zhou Q, Han R, Song Y, Cheng H Tags: Gene Source Type: research

Identification and Functional Analysis of Fourteen NR5A1 Variants in Patients with 46 XY Disorders of Sex Development.
In this study, 11 NR5A1 pathogenic variants were identified. These novel variants further expand the existing spectrum of the NR5A1 variants associated with 46,XY DSD, which will, in turn, assist in the molecular diagnosis of DSD. PMID: 32738419 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 29, 2020 Category: Genetics & Stem Cells Authors: Na X, Mao Y, Tang Y, Jiang W, Yu J, Cao L, Yang J Tags: Gene Source Type: research

Telomerase inhibition regulates EMT mechanism in breast cancer stem cells.
CONCLUSION: Our obtained results indicated that telomerase inhibition affects the EMT mechanism. The targeted elimination of breast cancer stem cells by a telomerase inhibitor in cancer treatment may limit the mobility and stemness of cancer cells due to the EMT mechanism, thus may prevent metastasis. PMID: 32738420 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 29, 2020 Category: Genetics & Stem Cells Authors: Kusoglu A, Bagca BG, Ay NPO, Gunduz C, Avci CB Tags: Gene Source Type: research

Recent Advancements in Understanding the Role of Epigenetics in the Auditory System.
The objective of this article is to review the existing literature and discuss the advancements made in understanding epigenetic modifications of inner ear sensory epithelial cells. The analysis of the emerging epigenetic mechanisms related to inner ear sensory epithelial cells development, differentiation, protection, and regeneration will pave the way to develop novel therapeutic strategies for hearing loss. PMID: 32738421 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 29, 2020 Category: Genetics & Stem Cells Authors: Mittal R, Bencie N, Liu G, Eshraghi N, Nisenbaum E, Blanton SH, Yan D, Mittal J, Dinh CT, Young JI, Gong F, Liu XZ Tags: Gene Source Type: research

Novel tomato miRNA miR1001 initiates cross-species regulation to suppress the conidiospore germination and infection virulence of Botrytis cinerea in vitro.
This study investigated the regulatory role of novel tomato miRNA miR1001 in the growth and development of Botrytis cinerea. Results showed that miR1001 inhibited the virulence of B. cinerea-infected plants, and the inhibitory effect of miR1001/miR1001* was stronger than that of miR1001. Moreover, miR1001 exerted a significant inhibitory effect on the conidiospore germination of B. cinerea. Degradome-seq experiment showed that miR1001 can directly target the Bcin03g02170.1 and Bcin10g01400.1 genes, which respectively encode the ATP-dependent metallopeptidase and cysteine-type endopeptidase, in B. cinerea. The interactions ...
Source: Gene - July 26, 2020 Category: Genetics & Stem Cells Authors: Meng X, Jin W, Wu F Tags: Gene Source Type: research

Silencing circ_0001879 inhibits the proliferation and migration of human retinal microvascular endothelial cells under high-glucose conditions via modulating miR-30-3p.
CONCLUSION: Silencing circ_0001879 inhibited the proliferation and migration of HRMECs under high-glucose conditions via modulating miR-30-3p, which might shed new light on a novel potentially marker and molecular therapeutic target for diabetic retinopathy. PMID: 32721474 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 25, 2020 Category: Genetics & Stem Cells Authors: Zeng Q, Liu J Tags: Gene Source Type: research

MicroRNAs as regulators of VEGFA and NFE2L2 in cancer.
llo E Abstract MicroRNAs (miRNAs) are small non-coding RNAs that are involved in post-transcriptional regulation of various genes, and their deregulation can lead to tumorigenesis. They may play the role of oncogenes or tumor suppressors by regulating different genes involved in cellular processes. One of the genes regulated by the miRNAs is the vascular endothelial growth factor A (VEGFA), which is responsible for angiogenesis. Angiogenesis is the process of formation of new blood vessels from pre-existing ones. This process plays an important role in tumor development, since it is responsible for the transport o...
Source: Gene - July 25, 2020 Category: Genetics & Stem Cells Authors: Izak Cuzziol C, Maria Urbanin Castanhole-Nunes M, Cristina Pavarino É, Maria Goloni-Bertollo E Tags: Gene Source Type: research

Isolation and molecular characterization of NtMYB4a, a putative transcription activation factor involved in anthocyanin synthesis in tobacco.
Abstract The MYB transcription factors are involved in the regulation of plant secondary metabolism, cell development and morphogenesis, and stress response. Here, a full-length, 816-bp NtMYB4a cDNA, which encodes a protein comprising 271 amino acids, was isolated from tobacco leaves. Phylogenetic analysis revealed that NtMYB4a is most similar to Nicotiana. attenuata MYB4, followed by Eriobotrya japonica MYB4, and NtMYB4a clustered with transcriptional activators rather than repressors. Subcellular localization assays showed that NtMYB4 localized in the nucleus, membrane, and cytoplasm. Expression analyses reveale...
Source: Gene - July 25, 2020 Category: Genetics & Stem Cells Authors: Luo Q, Liu R, Zeng L, Wu Y, Jiang Y, Yang Q, Nie Q Tags: Gene Source Type: research

Role of microRNAs in the development, prognosis and therapeutic response of patients with prostate cancer.
Abstract Prostate cancer is the most common cancer in males in several regions. One of the major challenges in diagnosis and treatment of this cancer is how to identify men who harbor an increased risk of having clinically significant prostate cancer and how to assess response to therapy. Biomarkers, like microRNAs (miRNAs) are one of the new diagnostic/therapeutic tools for clinicians. Finding men at high risk of significant cancer is essential as they will mostly benefit from earlier diagnosis and treatment. At the same time, it is important to reduce the number of unnecessary invasive biopsies in men without (c...
Source: Gene - July 25, 2020 Category: Genetics & Stem Cells Authors: Ghafouri-Fard S, Shoorei H, Taheri M Tags: Gene Source Type: research

Cathepsin-L is Involved in Degradation of Fat Body and Programmed Cell Death in Bombyx mori.
PMID: 32717304 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Yang H, Zhang R, Zhang Y, Liu Q, Li Y, Gong J, Hou Y Tags: Gene Source Type: research

Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines.
Abstract Clostridium perfringens beta2 (CPB2), a key virulence factor, is produced by C. perfringens type C that is the main pathogenic microorganism causing diarrhea in piglets. However, little is known concerning the toxic damage effect of CPB2 on intestinal cells of piglets. In present study, CPB2 toxin obtained by genetic recombination technology was evaluated for its cytotoxicity property using the intestinal porcine epithelial (IPEC-J2) cells, which aims to attempt to understand and explain its mechanism of action in porcine small intestinal epithelial cells. IPEC-J2 cells were treated with different concent...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Luo R, Yang Q, Huang X, Yan Z, Gao X, Wang W, Xie K, Wang P, Gun S Tags: Gene Source Type: research

Polymorphisms of CSF1R and WISP1 genes are associated with severity of familial adenomatous polyposis in APC1311 pigs.
Abstract Hereditary familial adenomatous polyposis (FAP) in humans significantly increases the risk of development of colorectal cancer (CRC). Germline mutations in the APC (adenomatous polyposis coli) gene are responsible for FAP. Despite having the same causative mutation, the severity of the disease differs from patient to patient. The porcine FAP model carrying a truncating APC1311 mutation, orthologous to the dominant human mutation that leads to severe form of the disease (APC1309), mirrors the severity of polyposis. Earlier RNAseq studies have revealed the differential expression of WISP1 and CSF1R in sampl...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Sikorska A, Stachowiak M, Flisikowska T, Stachecka J, Flisikowski K, Switonski M Tags: Gene Source Type: research

Inhibition of kinesin motor protein KIFC1 by AZ82 induces multipolar mitosis and apoptosis in prostate cancer cell.
Abstract Kinesin 14 family member KIFC1 is a mitotic kinesin which contains a C-terminal motor domain and plays a vital role for clustering the amplified centrosomes. Overexpression of KIFC1 in prostate cancer (PCa) cells showed resistance to docetaxel (DTX). The present study revealed that small KIFC1 inhibitor AZ82 suppresed the transcription and translation of KIFC1 significantly in PCa cells. AZ82 inhibited the KIFC1 expression both in the cytoplasm and nucleus of PCa cells. Inhibition of KIFC1 by AZ82 caused multipolar mitosis in PCa cells via de-clustering the amplified centrosomes and decreased the rate of ...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Parvin A, Hao SL, Tan FQ, Yang WX Tags: Gene Source Type: research

Target sequencing identify loss of function variants in FSIP1 are associated with one particular subtype of mucosal melanoma.
CONCLUSIONS: In our study, a susceptibility gene for oral mucosal melanoma was confirmed in a Chinese Han population, and these findings contribute to a better genetic understanding of mucosal melanoma of different subtypes. PMID: 32717308 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Chen M, Wu Y, Li W, Zhang X, Chen L, Zheng X, Zuo X, Zhou F, Hong Y, Cheng H, Zhang M, Chen Z, Wu Q, Zhang X, Zhang B Tags: Gene Source Type: research

Current trends and opportunities in targeting p21 activated kinase-1(PAK1) for therapeutic management of breast cancers.
Abstract Breast cancer is the most frequently diagnosed cancer in women worldwide. Identifying reliable biomarkers and druggable molecular targets pose to be a significant quest in breast cancer research. p21-activated kinase 1 (PAK1) is a serine/threonine kinase that direct cell motility, cytoskeletal remodelling, and has been shown to function as a downstream regulator for various cancer signalling cascades that promote cell proliferation, apoptosis deregulation and hasten mitotic abnormalities, resulting in tumor formation and progression. The heterogeneity and acquired drug resistance are important factors tha...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Kanumuri R, Saravanan R, Pavithra V, Sundaram S, Rayala SK, Venkatraman G Tags: Gene Source Type: research

Rucaparib antagonize multidrug resistance in cervical cancer cells through blocking the function of ABC transporters.
Abstract Upregulation of the ATP-binding cassette (ABC) transporter is one of the most important factors leading to multidrug resistance (MDR) in several types of cancer. In the present study, we investigated the ability of rucaparib, a Poly (ADP-ribose) polymerase (PARP) inhibitor which is currently in clinical development, on overcoming ABC transporters-mediated MDR in cervical cancer cell lines. Rucaparib significantly enhanced the cytotoxic effects of a series of conventional chemotherapeutic drugs in drug resistance cervical cancer cell lines. Moreover, rucaparib significantly increased the accumulation of rh...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Chen Z, Ling K, Zhu Y, Deng L, Li Y, Liang Z Tags: Gene Source Type: research

CRISPR-Based Metabolic Editing: Next-Generation Metabolic Engineering in Plants.
Abstract Plants generate many secondary metabolites, so called phyto-metabolites, which can be used as toxins, dyes, drugs, and insecticides in bio-warfare plus bio-terrorism, industry, medicine, and agriculture, respectively. To 2013, the first generation metabolic engineering approaches like miRNA-based manipulation were widely adopted by researchers in biosciences. However, the discovery of the clustered regularly interspaced short palindromic repeat (CRISPR) genome editing system revolutionized metabolic engineering due to its unique features so that scientists could manipulate the biosynthetic pathways of phy...
Source: Gene - July 24, 2020 Category: Genetics & Stem Cells Authors: Sabzehzari M, Zeinali M, Reza Naghavi M Tags: Gene Source Type: research

Artesunate Ameliorates Sepsis-Induced Acute Lung Injury by Activating the mTOR/AKT/PI3K Axis.
Abstract Sepsis-induced acute lung injury (ALI) remains one of the most common disorders in hospitals. The aim of this research was to explore the underlying characteristics and mechanisms of artesunate (AS) in protecting rat lungs from sepsis. The sepsis-induced ALI model was generated in SD rats by the intratracheal administration of lipopolysaccharide (LPS, 5 mg/kg) for 24 h. The rats were randomly assigned into 4 groups: Sham, LPS, LPS + AS, and LPS + AS +LY294002. Pathological evaluation of the lungs was conducted by HE staining, immunofluorescence, and TUNEL assay, and the MPO activity and the W/D rat...
Source: Gene - July 23, 2020 Category: Genetics & Stem Cells Authors: Zhang E, Wang J, Chen Q, Wang Z, Li D, Jiang N, Ju X Tags: Gene Source Type: research

Effects of co-administration of rapamycin and evening primrose / hemp seed oil supplement on immunologic factors and cells membrane fatty acids of experimental autoimmune encephalomyelitis.
CONCLUSION: These results point to potential therapeutic effects on the repair of the structure of cell membranes by EPO / HSO in EAE. PMID: 32712065 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 23, 2020 Category: Genetics & Stem Cells Authors: Rezapour-Firouzi S, Mohammadian M, Sadeghzadeh M, Mazloomi E Tags: Gene Source Type: research

Molecular characterization of myostatin and its inhibitory function on myogenesis and muscle growth in Chinese Shrimp, Fenneropenaeus chinensis.
In this study, we identified and characterized the Mstn from Fenneropenaeus chinensis (FcMstn), and investigated its function on myogenesis and muscle growth. Two different cDNA sequences (2628 bp and 2604 bp) encoding for slightly different sizes of proteins were obtained for FcMstn, containing 86 bp of 5' untranslated regions (UTR) and 1258 bp of 3' UTR. The open reading frame of the long sequence and the short sequence contain 1284 bp and 1260 bp cDNA, encoding 427 and 419 amino acid sequence, respectively. Sequence analysis revealed that the overall protein sequence and specific functional sites of FcMstn were highly c...
Source: Gene - July 22, 2020 Category: Genetics & Stem Cells Authors: Yan Y, Lu X, Kong J, Meng X, Luan S, Dai P, Chen B, Cao B, Luo K Tags: Gene Source Type: research

The transcription factor CBFB mutations indicate an improved survival in HR+/HER2- breast cancer.
CONCLUSION: This study reveals race diversity of CBFB mutation spectrum in breast cancers. CBFB mutations mainly occur in HR+/HER2- breast cancer, and it may be a promising prognostic biomarker in HR+/HER2- subgroup. PMID: 32711101 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 22, 2020 Category: Genetics & Stem Cells Authors: Guo L, Chen B, Zhang G, Wang Y, Cao L, Ren C, Wen L, Lin J, Wei G, Liao N Tags: Gene Source Type: research

Characterizing transcriptome in female scallop Chlamys farreri provides new insights into the molecular mechanisms of reproductive regulation during ovarian development and spawn.
In this study, transcriptomes of ovary at four reproductive stages in female Chlamys farreri were characterized by RNA-Seq. Regarding signaling pathways, ECM-receptor interaction pathway, mTOR signaling pathway, Fanconi anemia pathway, FoxO signaling pathway, Wnt signaling pathway and Hedgehog signaling pathway were enriched during ovarian development processes. In addition, pathways related to energy metabolism such as Nitrogen metabolism and Arachidonic acid metabolism were enriched at spawn stage. Interestingly, Neuroactive ligand-receptor interaction was significantly enriched involved in ovarian development and spawn,...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Xu R, Pan L, Yang Y, Zhou Y Tags: Gene Source Type: research

Isolation, identification, expression and subcellular localization of PPARG gene in buffalo mammary gland.
In this study, the transcripts of PPARG gene were isolated and identified in buffalo mammary gland. The results showed that two types of transcripts (PPARG1 and PPARG2) of PPARG gene produced by alternative 5' end use were expressed in buffalo mammary gland, and each of them had four different alternative splicing variants. The PPARG1 includes PPARG1a, PPARG1b, PPARG1c and PPARG1d, while the PPARG2 contains PPARG2a, PPARG2b, PPARG2c and PPARG2d. Among them, only PPARG1a, PPARG2a and PPARG2d can encode complete functional proteins with three complete functional domains, and the rest encode truncated proteins with incomplete...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Zhou F, Teng X, Wang P, Zhang Y, Miao Y Tags: Gene Source Type: research

Circ_0123996 promotes cell proliferation and fibrosisin mouse mesangial cells through sponging miR-149-5p and inducing Bach1 expression.
Abstract Diabetic nephropathy (DN) is a serious microvascular complication of diabetes across the world. Recently, many circular RNAs (circRNAs) can exert a crucial role in DN progression. Our investigation was designed to study whether circ_0123996 was associated with DN and aimed to find out the underlying mechanisms. We observed that circ_0123996 expression was significantly increased in Type 2 diabetes (T2D) with DN in comparison to those patients without DN. Consistently, circ_0123996 was also obviously elevated in DN mice models and high glucose (HG)-incubated MMCs. Then, it was proved transfection of circ_0...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Wang W, Feng J, Zhou H, Li Q Tags: Gene Source Type: research

Brain Transcriptome Study through CRISPR/Cas9 mediated Mouse Dip2c gene Knock-out.
Abstract Dip2C is highly expressed in brain and many other tissues but its biological functions are still not clear. Genes regulated by Dip2C in brain have never been studied. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, adaptive immune systems of bacteria and archaea, have been recently developed and broadly used in genome editing. Here, we describe targeted gene deletions of Dip2c gene in mice via CRISPR/Cas9 system and study of brain transcriptome under Dip2C regulation. The CRISPR/Cas9 system effectively generated targeted deletions of Dip2c by ...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Mar Oo Z, Adlat S, Kumar Sah R, Zun Zaw Myint M, Hayel F, Chen Y, Htoo H, Binta Bah F, Bahadar N, Kaythi Chan M, Zhang L, Feng X, Zheng Y Tags: Gene Source Type: research

Dexmedetomidine Ameliorates Renal Ischemia Reperfusion-Mediated Activation of the NLRP3 Inflammasome in Alveolar Macrophages.
In conclusion, our study demonstrated that the protective action of DEX in AMs is induced through amelioration of HMGB1-NLRP3 inflammasome-AMPK signaling. Our results suggest that the anesthetic reagent DEX exerts beneficial effects to ameliorate rI/R-induced ALI. PMID: 32707303 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Chen Y, Huang Y, Xiong B, Luo H, Song X Tags: Gene Source Type: research

Comparative Investigation of Coarse and Fine Wool Sheep Skin Indicates the Early Regulators for Skin and Wool Diversity.
Abstract The hair follicle is an excellent mini-system illustrating the mechanisms governing organogenesis and regeneration. Although the general mechanisms modulating skin and hair follicle development are widely studied in mouse and chicken models, the delicate network regulating skin and hair diversity remains largely unclear. Sheep is an additional model to address the various wool characteristics observed in nature. The coarse and fine wool sheep with diverse fibers were examined to show differences in the primary wool follicle size and skin thickness. The molecular dynamics in skin staged at the primary wool...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Li S, Chen W, Zheng X, Liu Z, Yang G, Hu X, Mou C Tags: Gene Source Type: research

Establishment and validation of a novel autophagy-related gene signature for patients with breast cancer.
This study aimed to construct and evaluate a novel autophagy-related gene signature as a potential prognostic factor and therapeutic target in breast cancer patients based on high-throughput sequencing datasets. MATERIALS & METHODS: Autophagy-related genes obtained from the Human Autophagy Database and high-sequencing data obtained from The Cancer Genome Atlas (TCGA) were analyzed to identify differential expressed genes (DEGs) between tumor and normal tissues. Then GO and KEGG analysis were performed to explore potential biological and pathological functions of DEGs. Autophagy-related prognostic genes were identi...
Source: Gene - July 21, 2020 Category: Genetics & Stem Cells Authors: Du JX, Chen C, Luo YH, Cai JL, Cai CZ, Xu J, Ni XJ, Zhu W Tags: Gene Source Type: research

Full-length sequencing of ginkgo transcriptomes for an in-depth understanding of flavonoid and terpenoid trilactone biosynthesis.
Abstract Ginkgo biloba L. is regarded as the most ancient living tree, and its kernel has been used as a traditional Chinese medicine for more than 2,000 years. The leaf extracts of this tree have been among the bestselling herbal remedies in Western countries since the last century. To understand the biosynthesis of the pharmacologically active ingredients in G. biloba, flavonoids and terpenoid trilactones (TTLs), we sequenced the transcriptomes of G. biloba leaves, kernels and testae with Iso-Seq and RNA-Seq technologies and obtained 152,524 clean consensus reads. When these reads were used to improve the annota...
Source: Gene - July 18, 2020 Category: Genetics & Stem Cells Authors: Sun S, Li Y, Chu L, Kuang X, Song J, Sun C Tags: Gene Source Type: research

Cohesin Subunit RAD21: from Biology to Disease.
Abstract RAD21 (also known as KIAA0078, NXP1, HR21, Mcd1, Scc1, and hereafter called RAD21), an essential gene, encodes a DNA double-strand break (DSB) repair protein that is evolutionarily conserved in all eukaryotes from budding yeast to humans. RAD21 protein is a structural component of the highly conserved cohesin complex consisting of RAD21, SMC1a, SMC3, and SCC3 [STAG1 (SA1) and STAG2 (SA2) in metazoans] proteins, involved in sister chromatid cohesion. This function is essential for proper chromosome segregation, post-replicative DNA repair, and prevention of inappropriate recombination between repetitive re...
Source: Gene - July 17, 2020 Category: Genetics & Stem Cells Authors: Cheng H, Zhang N, Pati D Tags: Gene Source Type: research

Mitonuclear interactions influence multiple sclerosis risk.
Abstract Multiple sclerosis (MS) is a chronic disease of the central nervous system characterized by the autoimmune inflammation, demyelination, and neurodegeneration. This complex disease develops in genetically predisposed individuals under adverse environmental factors. To date, a large number of MS-associated polymorphic loci of the nuclear genome have been identified; however, their total variability can explain only about 48% of the observed inheritance of MS. Polymorphic variants of the mitochondrial genome and interactions of mitochondrial and nuclear genes (mitonuclear interactions) may be the possible so...
Source: Gene - July 17, 2020 Category: Genetics & Stem Cells Authors: Kozin M, Kulakova O, Kiselev I, Baulina N, Boyko A, Favorova O Tags: Gene Source Type: research

The USP22 promotes the growth of cancer cells through the DYRK1A in pancreatic ductal adenocarcinoma.
Abstract As a member of the ubiquitin-specific protease (USP) family, USP22 could remove ubiquitin moieties from its target proteins to control the function of the target proteins. Accumulating studies show that USP22 essentially participates in diverse types of cancer as an oncogene-like protein. However, the roles of USP22 in human pancreatic ductal adenocarcinoma (PDAC) and the underlying mechanism are unknown. Here we report that USP22 promotes the growth of PDAC cells by promoting the expression of dual-specificity tyrosine regulated kinase 1A (DYRK1A). Our results showed that the expression levels of USP22 w...
Source: Gene - July 17, 2020 Category: Genetics & Stem Cells Authors: Bai Z, Du Y, Cong L, Cheng Y Tags: Gene Source Type: research

Silencing ICAM-1 reduces the adhesion of vascular endothelial cells in mice with immunologic contact urticaria.
CONCLUSION: These findings indicate that RNAi-mediated gene silencing of ICAM-1 may decrease VECs adhesion expression and reduce cell-cell adhesion in mice with ICU. PMID: 32687948 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 17, 2020 Category: Genetics & Stem Cells Authors: Lv G, Fan J Tags: Gene Source Type: research

Lack of Methylation on Transgene Leads to High Level and Persistent Transgene Expression in induced Pluripotent Stem Cells.
In this study, we assessed the implication of DNA methylation on the duration of GFP expression in mouse embryonic stem (ES) and mouse induced pluripotent stem (iPS) cells. The cells were transduced with lentivirus (LV) carrying green fluorescent protein (GFP) driven by either human elongation factor (EF1α) or cytomegalovirus (CMV) promoter. Transduced iPS cells exhibited higher percentage of GFP+ cells with persistent mean fluorescent intensity than transduced ES cells. Analysis on the integrated copy of transgene in the population of the transduced cells demonstrated similar copy number. However, significant increa...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Yusuf Alhaji S, Nordin N, Ching Ngai S, Al Abbar A, Mei L, Abdullah S Tags: Gene Source Type: research

Circular RNA Circ_0000064 promotes the proliferation and fibrosis of mesangial cells via miR-143 in diabetic nephropathy.
In conclusion, we demonstrated the regulatory role of circ_0000064 in DN and clarified that circ_0000064 play a role in DN via targeting miR-143. Circ_0000064 and miR-143 also showed the potential as a biomarker for DN. PMID: 32683074 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Ge X, Xi L, Wang Q, Li H, Xia L, Cang Z, Peng W, Huang S Tags: Gene Source Type: research

Candidate Gene Association Analysis of Multiple Sclerosis in the Jordanian Arab Population: A Case-Control Study.
Abstract Multiple sclerosis (MS) is a clinically heterogeneous multifactorial disorder which is one of the most prevalent neurological disorders of females and young people. Both genetic and environmental factors are playing an important role in the pathophysiology of MS. The main objective of this study is to identify the relationship between numbers of genetic variants within different candidate genes (IL7R, LAG3, and CD40) and the risk of developing MS in the Jordanian Arab population. This case-control study consists of 218 MS patients chosen from neurology clinics at different hospitals in Jordan and ethnical...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Al-Eitan L, Al Qudah M, Al Qawasmeh M Tags: Gene Source Type: research

Cyclin B protein undergoes increased expression and nuclear relocation during oocyte meiotic maturation of the freshwater prawn Macrobrachium rosenbergii and the Chinese mitten crab Eriocheir sinensis.
Abstract Cyclin B functions as a regulatory protein through association with its catalytic partner Cdc2 kinase forming M-phase promoting factor (MPF), which plays a central role in the meiotic maturation of oocyte. To gain insight into the molecular events, we here cloned a cyclin B cDNA from the ovary of the prawn Macrobrachium rosenbergii and compared its spatial-temporal expression patterns during oocyte maturation with those of crab Eriocheir sinensis. The prawn cyclin B cDNA encodes a 398 amino acid protein with predicted molecular weight of 45.16 kDa. Immunodetection of cyclin B protein by Western blot showe...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Feng H, Dong YT, Liu X, Qiu Writing-Review GF, Editing Tags: Gene Source Type: research

Comparative Analysis of epi-miRNA Expression Levels in Local/Locally Advanced and Metastatic Prostate Cancer Patients.
In this study, the expression levels of 30 epi-miRNAs in whole blood samples from 25 control, 25 PCa and 40 metastatic PCa patients were investigated by the Quantitative Real-Time PCR method. Then, promoter methylation levels of 11 epi-miRNAs, whose expression levels were found to be significantly higher, were examined by methylation-specific qPCR method. The correlations between miRNA expression levels and clinicopathological parameters (Gleason Score (GS), PSA levels, TNM Staging) in different stages of PCa groups as well as disease-specific expression levels were examined. We found a hypomethylation in the promoter regi...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Gurbuz V, Kiliccioglu I, Ugras Dikmen A, Bilen CY, Sozen S, Konac E Tags: Gene Source Type: research

Functional Characterization of Cinnamate 4-hydroxylase from Helianthus annuus Linn Using a Fusion Protein Method.
In this study, we screened three C4H genes from the sunflower transcriptome and genomic databases, named HaC4H1, HaC4H2, and, HaC4H3, respectively. In heterologous expression experiments, we had improved a method from previous studies by the addition of restriction sites to make it easier to express multiple C4H functions and suitable for in vitro activity verification. HaC4Hs without the N-terminal membrane anchor region was fused with a redox partner of Arabidopsis thaliana cytochrome P450 enzyme (CYP450) by the method and functionally expressed in E. coli and the results showed that these three enzymes catalyzed the for...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Wang Z, Jian X, Zhao Y, Li S, Sui Z, Li L, Kong L, Luo J Tags: Gene Source Type: research

Heterologous overexpression of the GbTCP5 gene increased root hair length, root hair and stem trichome density, and lignin content in transgenic Arabidopsis.
In this study, the GbTCP5 gene encodes a sea-island cotton class II TCP CIN subclass transcription factor. The GbTCP5 transcription factor is located in the nucleus, has transcriptional activation activity, and can bind to TCP II cis-acting elements. GbTCP5 was widely expressed in tissues with the highest transcript level in the calyx. GbTCP5 is expressed at different developmental stages of the fiber and has significantly high transcriptional level expression in the fibers at 20, 30 and 35 days post anthesis (DPA). Heterologous overexpression of the GbTCP5 gene increased root hair length, root hair and stem trichome densi...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Wang Y, Yu Y, Wang J, Chen Q, Ni Z Tags: Gene Source Type: research

Metagenomic study focusing on antibiotic resistance genes from the sediments of River Yamuna.
In this study, metagenomics approach was employed a for constructing a comprehensive profile of the Antibiotic Resistance Genes (ARGs) identified in the sediments of the Yamuna River. A total of 139 ARGs were identified from 39 microbial species. Abundance analysis revealed that, aminoglycoside, beta-lactam, macrolide, and tetracycline resistance genes were highly abundant in the sediment samples obtained from the Yamuna River. The evolutionary relationships among the ARGs were studied by phylogenetic analyses, which revealed that, the identified resistome comprised eight clusters. Network analysis was performed for invest...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Kumar Das B, Kumar Behera B, Jyoti Chakraborty H, Paria P, Gangopadhyay A, Kumar Rout A, Kumari Nayak K, Kumar Parida P, Rai A Tags: Gene Source Type: research

Detection of polymorphisms in the bovine leptin receptor gene affects fat deposition in two Chinese beef cattle breeds.
This study investigated the presence and frequency of polymorphisms of bovine LEPR gene and determine whether the polymorphisms are associated with the fat deposition in two Chinese beef cattle breeds. Quantitative real-time polymerase chain reactions identified that the expression of LEPR gene was highest in the liver and subcutaneous fat. Four single nucleotide polymorphisms (SNPs) were identified including g.24169 C>T, g.24256 T>A, g.24267 G>C and g.24413 T>A. A greater backfat thickness was associated with the AA genotype of g.24256 T>A compared to the TT genotype. A greater intramuscular fat content was...
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Raza SHA, Liu GY, Zhou L, Gui LS, Khan R, Jinmeng Y, Chugang M, Schreurs NM, Ji R, Zan L Tags: Gene Source Type: research

Circ_0001946 facilitates the proliferation and represses apoptosis of ox-LDL-stimulated Vascular Smooth Muscle Cells via regulating miR-641/CCND1 pathway.
In conclusion, our current data suggested circ_0001946 silence inhibited VSMCs proliferation and increased cell apoptosis through modulating miR-641 and CCND1. PMID: 32683082 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 16, 2020 Category: Genetics & Stem Cells Authors: Cai XY, Shao L, Zhu HM, Zhan YL, Wang YX, Tu WL, Hong L, Wang S Tags: Gene Source Type: research

Emerging roles of melanocortin receptor accessory proteins (MRAP and MRAP2) in physiology and pathophysiology.
Abstract In summary, genetic variants of MRAP are associated with severe familial glucocorticoid deficiency, however evidence is emerging that synthetic glucocorticoids may also disrupt ACTH and MRAP expression resulting in adrenal inflammation. MRAP2 genetic variants are associated with severe obesity but the phenotypes are subtly different to the phenotypes observed with MC4R genetic variants. There is a great deal of interest in the development of melanocortin therapeutics to treat obesity, as evidenced by setmelanotide, and fully understanding the role of MRAP2 and the interactions with both melanocortin recep...
Source: Gene - July 14, 2020 Category: Genetics & Stem Cells Authors: Berruien NNA, Smith CL Tags: Gene Source Type: research

FoxM1 promotes the migration of ovarian cancer cell through KRT5 and KRT7.
Abstract Forkhead box M1(FoxM1) played an important role in the pathogenesis of ovarian cancer, but its downstream molecular network is mysterious. Here, we combined ChIP-seq with RNA-seq analysis and identified 687 FoxM1-binding regions and 182 genes regulated by FoxM1. The above data pointed out that KRT5 and KRT7 were downstream target genes of FoxM1. Next, we used qPCR and Western blot to verify that FoxM1 knockdown inhibited the expression levels of KRT5 and KRT7. We also demonstrated that FoxM1 regulated KRT5 and KRT7 genes expression through binding a consensus AP-2 cis element, and showed that KRT5 and KRT...
Source: Gene - July 10, 2020 Category: Genetics & Stem Cells Authors: Zhang Z, Tu K, Liu F, Liang M, Yu K, Wang Y, Luo Y, Yang B, Qin Y, He D, Jiang G, Huang O, Zou Y Tags: Gene Source Type: research

VcMYB4a, an R2R3-MYB transcription factor from Vaccinium corymbosum, negatively regulates salt, drought, and temperature stress.
In this study, we cloned a novel MYB TF gene from Vaccinium corymbosum (blueberry) using rapid amplification of cDNA ends (RACE). The cDNA contained a 798-bp open reading frame that encodes a 265-amino acid protein. VcMYB4a possessed a C2/EAR-repressor motif domain and phylogenetic analysis showed that it clustered into a subgroup 4 with six Arabidopsis thaliana MYBs. Quantitative RT-PCR analysis demonstrated that VcMYB4a expression was downregulated by salt, drought, and cold treatment, but was induced by freezing and heat. Overexpression of VcMYB4a in blueberry callus enhanced sensitivity to salt, drought, cold, freezing...
Source: Gene - July 9, 2020 Category: Genetics & Stem Cells Authors: Zhang CY, Liu HC, Zhang XS, Guo QX, Bian SM, Wang JY, Zhai LL Tags: Gene Source Type: research

Overexpression of the Tuberous sclerosis complex 2 (TSC2) gene inhibits goat myoblasts proliferation and differentiation in understanding the underlying mechanism of muscle development.
Abstract The growth of animal skeletal muscle is mainly determined by the synthesis processes of total proteins in skeletal muscle cells, which has a significant impact on the postnatal growth of young animals. An increasing number of studies are focusing on the functions of Tuberous sclerosis complex 2 (TSC2) during the process of cell protein synthesis and growth. However, it is still unclear the effect of whether and how TSC2 on goat myoblasts proliferation and differentiation. Here, we found that TSC2 gene has opposite expression patterns in proliferation and differentiation of myoblasts. An expression vector ...
Source: Gene - July 8, 2020 Category: Genetics & Stem Cells Authors: Lyu M, Shalitana A, Luo J, He H, Sun S, Wang P Tags: Gene Source Type: research

Implication of Pseudo Reference Genes in Normalization of Data from Reverse Transcription-Quantitative PCR.
Abstract Pseudogenes are duplicated or retrotransposed DNA sequences of native functional genes. Amplification of pseudogenes along with gene of interest often produces false positive results, which is an innate problem in Reverse Transcription-quantitative PCR (RT-qPCR). Selecting a reference gene without any interference from pseudogene amplification is therefore a challenge to overcome. Among the common reference genes used for normalization (ACTB, GAPDH, HPRT1, TUBB, 18SrRNA and B2M), B2M was found to have no pseudogenes in silico, which has also been confirmed by RT-qPCR. We also assessed the effect of pseudo...
Source: Gene - July 8, 2020 Category: Genetics & Stem Cells Authors: Balaji S, Vanniarajan A Tags: Gene Source Type: research