Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment.
Abstract The CONSTANS-LIKE (COL) gene has an important role both in regulation flowering through photoperiodic control and response to abiotic stress. In the present study, we performed a genome-wide analysis of maize COL gene family and identified 19 non-redundant ZmCOL genes, which were unequally distributed on ten maize chromosomes. Analysis of compound phylogenetic tree (maize, sorghum, rice and Arabidopsis) showed high bootstrap, as well as conserved domain and semblable gene structures among members within the same clade, revealing that COL genes in same clade were from the common ancestral and prior to the ...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Song N, Xu Z, Wang J, Qin Q, Jiang H, Si W, Li X Tags: Gene Source Type: research

LncRNA UCA1 sponges miR-26a to regulate the migration and proliferation of vascular smooth muscle cells.
CONCLUSION: The expression of UCA1 antagonized the effect of miR-26a on the downregulation of its target PETN and contraction phenotype. This study reveals that lncRNA UCA1 act as an endogenous sponge of miR-26a and downregulates miR-26a expression levels, and thereby relieving the inhibition of its target gene PTEN and alleviates VSMCs proliferation against atherosclerosis. PMID: 29908280 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Tian S, Yuan Y, Li Z, Gao M, Lu Y, Gao H Tags: Gene Source Type: research

LTBP2 knockdown and oxidative stress affect glaucoma features including TGF β pathways, ECM genes expression and apoptosis in trabecular meshwork cells.
LTBP2 knockdown and oxidative stress affect glaucoma features including TGFβ pathways, ECM genes expression and apoptosis in trabecular meshwork cells. Gene. 2018 Jun 13;: Authors: Suri F, Yazdani S, Elahi E Abstract Glaucoma is the leading cause of irreversible blindness worldwide. Although the etiology of glaucoma is incompletely understood, it is known that the extracellular matrix (ECM) of the trabecular meshwork, oxidative stress, TGFβ signaling pathways, and apoptosis are important components of glaucoma pathogenesis. These components appear to be interrelated, but knowledge on their i...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Suri F, Yazdani S, Elahi E Tags: Gene Source Type: research

The function and clinical significance of eIF3 in cancer.
Abstract Abnormal regulation of gene expression is essential for tumorigenesis. Several studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by subunits of eukaryotic translation initiation factors (eIFs). Eukaryotic translation initiation factor 3 (eIF3) is the largest (800 kDa) and the most complex mammalian initiation factor. It is composed of 13 non-identical polypeptides designated as eIF3a-m and plays a pivotal role in protein synthesis that bridges the 43S pre-initiation complex and eIF4F-bound mRNA. However, the functional roles of individual subunits are n...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Yin Y, Long J, Sun Y, Li H, Jiang E, Zeng C, Zhu W Tags: Gene Source Type: research

MiR-378 and BMP-Smad can influence the proliferation of sheep myoblast.
Abstract MicroRNA (miRNA) is a sort of endogenous ~20-25 nt non-coding RNAs, and it can regulate a variety of biological events. We found the miR-378 may involve in regulating the muscle development of sheep during our previous research. However, the molecular mechanism of miR-378 regulating myoblast proliferation is still unclear. In this research, we predicted that BMP2 (Bone morphogenetic protein 2) was the target gene of miR-378 and the BMP-Smad signal pathway that BMP2 participated in playing an important role in the muscle development. Therefore, we tried to determine whether miR-378 influence myoblast pro...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Lu Z, Du L, Liu R, Di R, Zhang L, Ma Y, Li Q, Liu E, Chu M, Wei C Tags: Gene Source Type: research

Downregulation of SCARA5 may contribute to breast cancer via promoter hypermethylation.
Abstract Breast cancer is the most common malignant tumor in women worldwide. Breast tumors mostly exhibit aberrant gene expression and DNA hypermethylation patterns that predispose the disease. Understanding the genetic and epigenetic factors that contribute to breast cancer development is important to identify novel diagnostic and prognostic markers. SCARA5: Scavenger receptor class A, member 5; is a member of the scavenger receptor family located on chromosome 8p21 which is a frequently deleted region in human cancers. SCARA5 has been identified as a candidate tumor suppressor gene in various kinds of cancer. H...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Ulker D, Ersoy YE, Gucin Z, Muslumanoglu M, Buyru N Tags: Gene Source Type: research

Evaluating the effect of multiple genetic risk score models on colorectal cancer risk prediction.
In this study, we applied both simulation and real data to comprehensively compare the discriminability of different GRS models. The GRS models were fitted by logistic regression with three scenarios, including simple count GRS (SC-GRS), logistic regression weighted GRS (LR-GRS, including DL-GRS and OR-GRS) and explained variance weighted GRS (EV-GRS, including EV_DL-GRS and EV_OR-GRS) models. The model performance was evaluated by receiver operating characteristic (ROC) curves and area under curves (AUC) metric, net reclassification improvement (NRI) and integrated discrimination improvement (IDI). In real data analysis, ...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Xin J, Chu H, Ben S, Ge Y, Shao W, Zhao Y, Wei Y, Ma G, Li S, Gu D, Zhang Z, Du M, Wang M Tags: Gene Source Type: research

KatG plays an important role in Aeromonas hydrophila survival in fish macrophages and escape for further infection.
Abstract The success of the pathogenic bacteria is partly attributable to their ability to thwart host innate immune responses, which includes resisting the antimicrobial functions of macrophages. And reactive oxygen species (ROS) is one of the most effective antimicrobial components of macrophages to kill invading bacteria. Our previous studies found that Aeromonas hydrophila can survive in fish macrophages, which suggested that this bacterium might take fish macrophages as their shelters to resist drug killings and other immune damage. But how A. hydrophila survive in host macrophages remains unknown. Since KatG...
Source: Gene - June 12, 2018 Category: Genetics & Stem Cells Authors: Zhang M, Yan Q, Mao L, Wang S, Huang L, Xu X, Qin Y Tags: Gene Source Type: research

Association between matrix metalloproteinase family gene polymorphisms and risk of ischemic stroke: A systematic review and meta-analysis of 29 studies.
Abstract BACKGROUND: Ischemic stroke (IS) is a complex and devastating vascular disease that has become one of the leading causes of disability and mortality worldwide. Several studies have shown the association between matrix metalloproteinase (MMP) family gene polymorphisms and IS. However, the results have been indecisive. OBJECTIVE: To investigate the association between Matrix Metalloproteinase gene polymorphisms and risk of IS. METHODS: A literature search for eligible candidate gene studies published before, 28 June 2017, was conducted in the PubMed, EMBASE, Cochrane and Google Scholar databases. ...
Source: Gene - June 12, 2018 Category: Genetics & Stem Cells Authors: Misra S, Talwar P, Kumar A, Kumar P, Sagar R, Vibha D, Pandit AK, Gulati A, Kushwaha S, Prasad K Tags: Gene Source Type: research

Combination with TMZ and miR-505 inhibits the development of glioblastoma by regulating the WNT7B/Wnt/ β-catenin signaling pathway.
In conclusion, our results demonstrated that the induction of miR-505 in combination with TMZ treatment could be a useful therapeutic strategy for suppressing GBM. PMID: 29906532 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 12, 2018 Category: Genetics & Stem Cells Authors: Zhang C, Yang X, Fu C, Liu X Tags: Gene Source Type: research

Two novel COL6A3 mutations disrupt extracellular matrix formation and lead to myopathy from Ullrich congenital muscular dystrophy and Bethlem myopathy spectrum.
This study not only broadens allelic spectrum of pathogenic COL6A3 variants in myopathy, but also gives an additional support to Ullrich congenital muscular dystrophy Bethlem myopathy clinical continuum. PMID: 29894794 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 9, 2018 Category: Genetics & Stem Cells Authors: Marakhonov AV, Tabakov VY, Zernov NV, Dadali EL, Sharkova IV, Skoblov MY Tags: Gene Source Type: research

The role of CDKN2B in cardiovascular risk in ethnic Saudi Arabs: A validation study.
Abstract BACKGROUND: Genome-wide association studies (GWASs) have yielded a wealth of information furnishing support for the variability in genetic predisposition to disease. However, the actual impact of such findings on any particular ethnic population needs to be validated through replication studies. In the present study, we verified recent findings of a GWAS demonstrating a strong association for the cyclin-dependent kinase 4 inhibitor B (CDKN2B) genomic region with coronary artery disease (CAD)/myocardial infarction (MI) in ethnic Saudi Arabs. METHODOLOGY: We genotyped 8 CDKN2B SNPs for cardiovascular r...
Source: Gene - June 9, 2018 Category: Genetics & Stem Cells Authors: AlRasheed MM, Hefnawy MM, Elsherif NN, Alhawassi TM, Abanmy NO, AlRasheed NM, Alqahtani FY, Aleanizy FS, Muiya P, Al-Boudari OM, Dzimiri N Tags: Gene Source Type: research

Corrigendum to "Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain" [GENE 655 (2018) 1-12].
Corrigendum to "Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain" [GENE 655 (2018) 1-12]. Gene. 2018 Jun 08;: Authors: Das V, Kc R, Li X, Varma D, Qiu S, Kroin JS, Forsyth CB, Keshavarzian A, van Wijnen AJ, Park TJ, Stein GS, O-Sullivan I, Burris TP, Im HJ PMID: 29890121 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Das V, Kc R, Li X, Varma D, Qiu S, Kroin JS, Forsyth CB, Keshavarzian A, van Wijnen AJ, Park TJ, Stein GS, O-Sullivan I, Burris TP, Im HJ Tags: Gene Source Type: research

Association between MMP-9 -1562 C/T polymorphism and susceptibility to digestive cancers: A meta-analysis.
CONCLUSIONS: Our meta-analysis suggested that MMP-9 -1562 C/T polymorphism might be related to the digestive cancer susceptibility. Evidence with adequate sample size is needed. PMID: 29890308 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Chaoliang H, Fangzhong W, Lin L, Wei D, Jun Y, Liqing P, Ruixiang Z Tags: Gene Source Type: research

Genetic variants in the promoter region of the KLF3 gene associated with fat deposition in Qinchuan cattle.
Abstract Krüppel-like factor 3 (KLF3), a member of the Krüppel-like factor (KLF) family, plays an important role in adipogenesis and lipid metabolism. The aim of this study was to investigate whether KLF3 could be used as a candidate gene in the breeding of cattle. The expression pattern of bovine KLF3 gene revealed that it was highly expressed in abdominal fat and perirenal fat. Using DNA sequencing, three single nucleotide polymorphisms (SNPs) within the promoter regions of KLF3 gene were identified in 448 Qinchuan cattle, which are located in the recognition sequences of 11 transcription factors and t...
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Guo H, Raza SHA, Schreurs NM, Khan R, Wei D, Wang L, Zhang S, Zhang L, Wu S, Ullah I, Hosseini SM, Zan L Tags: Gene Source Type: research

Association of FoxP3 promoter polymorphisms with the risk of Graves' disease in ethnic Kashmiri population.
Abstract Graves' disease is a multifactorial autoimmune disorder of the thyroid gland, with some extra-thyroidal complications like eye and skin abnormalities in some patients. GD is more prevalent in women than men and is the leading cause of hyperthyroidism worldwide. A complex interaction between genetic and environmental factors is the proposed cause which triggers immune system to produce autoantibodies stimulating the TSH receptor, leading to clinical manifestations such as hyperthyroidism, diffuse thyroid enlargement (goiter) and often ophthalmopathy in affected individuals. Various Single nucleotide gene p...
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Shehjar F, Afroze D, Misgar RA, Malik SA, Laway BA Tags: Gene Source Type: research

Long non-coding RNA expression profile in Cdk5-knockdown mouse skin.
Abstract To elucidate the Cdk5 regulatory molecular mechanism in skin, we generated Cdk5-knockdown mice and subjected their skins to lncRNA sequencing. The results showed that there were 4533 novel lncRNAs from 142 lncRNA families. In total, 693 lncRNAs were significantly differentially expressed. Alignment analysis of the lncRNAs in miRBase identified 45 pre-mRNAs. By KEGG PATHWAY Database analysis, we found that lncRNAs (lnc-NONMMUT064276.2, lnc-NONMMUT075728.1, and lnc-NONMMUT039653.2) may regulate pigmentation by regulating target genes. To reveal potential antisense lncRNA-mRNA interactions, we searched all l...
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Ji K, Fan R, Zhang J, Yang S, Dong C Tags: Gene Source Type: research

The characterization and potential roles of bone morphogenetic protein 7 during spermatogenesis in Chinese mitten crab Eriocheir sinensis.
In this study, the whole gene encoding BMP7 protein was cloned and characterized firstly in Chinese mitten crab Eriocheir sinensis. The bioinformatics analysis of the deduced amino acid sequence showed that Es-BMP7 was composed of prodomain/latency-associated peptide and the TGF-β characteristic domain. The sequence conservation and phylogenetic analysis were also conducted. Quantitative real-time PCR was conducted indifferent tissues. The highest expression in testis indicated the potential role of BMP7 to male gonad development. Western blot results showed the different translational levels of BMP7 in different tiss...
Source: Gene - June 8, 2018 Category: Genetics & Stem Cells Authors: Xu YR, Wang GY, Zhou YC, Yang WX Tags: Gene Source Type: research

Down-regulation of NAMPT expression by mir-206 reduces cell survival of breast cancer cells.
Abstract Nicotinamide adenine dinucleotide (NAD) is a critical coenzyme for all living cells. Nicotinamide phosphoribosyltransferase (NAMPT) functions as a key enzyme in the salvage pathway of NAD biosynthesis. Cancer cells have higher rate of NAD consumption and therefore NAMPT is essential for their survival. Thus, we investigated the effect of NAMPT inhibition by miR-206 on breast cancer cell survival. Breast cancer cells were transfected with miR-206 mimic, inhibitor and their negative controls. NAMPT levels were assessed by real-time PCR as well as western blotting. Cell survival assay and quantification of N...
Source: Gene - June 7, 2018 Category: Genetics & Stem Cells Authors: Hesari Z, Nourbakhsh M, Hosseinkhani S, Abdolvahabi Z, Alipour M, Tavakoli-Yaraki M, Ghorbanhosseini SS, Yousefi Z, Jafarzadeh M, Yarahmadi S Tags: Gene Source Type: research

Rare genetic mutations in Pakistani patients with dilated cardiomyopathy.
In this study, whole exomes of five unrelated patients of idiopathic DCM were sequenced to an average depth of 100× using Illumina HiSeq4000 system. The analysis of the data with in silico tools SIFT, Polyphen2, and CADD showed 494 rare (AF 
Source: Gene - June 7, 2018 Category: Genetics & Stem Cells Authors: Shakeel M, Irfan M, Khan IA Tags: Gene Source Type: research

ATF3 and PRAP1 play important roles in cisplatin-induced damages in microvascular endothelial cells.
CONCLUSION: ATF3 and PRAP1 play important roles in cisplatin-induced DNA damage repair process. They may serve as potential early surrogate biomarkers of microvascular endothelial damage for cancer patients receiving chemotherapies. PMID: 29886035 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 7, 2018 Category: Genetics & Stem Cells Authors: Li M, Zhai G, Gu X, Sun K Tags: Gene Source Type: research

Molecular cloning and characterization of a sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) from Y-organs of the blue crab (Callinectes sapidus).
Abstract Existing data indicate that a Ca2+ signal stimulates ecdysteroid hormone production by crustacean molting glands (Y-organs). Ca2+ signaling is dependent on a tightly regulated Ca2+ gradient, with intracellular free Ca2+ maintained at a low basal level (typically sub-micromolar). This is achieved through the action of proteins intrinsic to the plasma membrane and the membranes of organelles. One such protein, the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), pumps Ca2+ from cytosol to the lumen of the endoplasmic reticulum. As a step toward understanding Ca2+-mediated regulation of ecdysteroidogenesis, ...
Source: Gene - June 7, 2018 Category: Genetics & Stem Cells Authors: Roegner ME, Chen HY, Douglas Watson R Tags: Gene Source Type: research

Identification of nicotianamine synthase genes in Triticum monococcum and their expression under different Fe and Zn concentrations.
Abstract In graminaceous plants, nicotianamine (NA) is an important component of metal acquisition. NA is synthesized from S-adenosyl-l-methionine (SAM) catalyzed by nicotianamine synthase (NAS). Here, eight Triticum monococcum NAS (TmNAS) genes were cloned and characterized. Amino acid sequence analysis showed that TmNAS genes had high sequence identity with those from Triticum aestivum, Zea mays, Oryza sativa and Hordeum vulgare. Phylogenetic analysis showed that NAS genes were classified into two distinct groups, e.g. group I and group II. Expression analysis demonstrated that two of the TmNAS genes in group II...
Source: Gene - June 6, 2018 Category: Genetics & Stem Cells Authors: Du X, Wang H, He J, Zhu B, Guo J, Hou W, Weng Q, Zhang X Tags: Gene Source Type: research

SIRT1 gene polymorphisms associated with decreased risk of atherosclerotic coronary artery disease.
Abstract Coronary artery disease (CAD) exhibits the rules of the multifactorial hereditary. Notwithstanding, the role of genetic factors in the prevalence of CAD is estimated very high. SIRT1 plays an athero-protective role and alterations in its expression have some important consequence in the metabolism, cholesterol, and fat deposition. The aim of our study was to investigate the association between rs4746720, rs12413112, and rs1467568 polymorphisms in the SIRT1 gene and CAD in the high-risk cases. In the present retrospective case-control study, 150 healthy individuals and 150 cases diagnosed with atherosclero...
Source: Gene - June 6, 2018 Category: Genetics & Stem Cells Authors: Nasiri M, Rauf M, Kamfiroozie H, Zibaeenezhad MJ, Jamali Z Tags: Gene Source Type: research

Evolutionary conservation and divergence of Vasa, Dazl and Nanos1 during embryogenesis and gametogenesis in dark sleeper (Odontobutis potamophila).
In this study, the full-length sequences of Opvasa, Opdazl and Opnanos1 were cloned and characterized from the dark sleeper (Odontobutis potamophila). Gonad-specific expression patterns of Opvasa and Opdazl were confirmed in adult tissues by quantitative real-time PCR (qRT-PCR). Different from Opvasa and Opdazl, the expression of Opnanos1 was ubiquitously detected in all examined tissues except for the liver and spleen. Time-course dynamic expressions during embryogenesis were assessed, and all three genes (Opvasa, Opdazl and Opnanos1) persisted at a high level until gastrulation. qRT-PCR and Western blotting analyses reve...
Source: Gene - June 6, 2018 Category: Genetics & Stem Cells Authors: Zhu W, Wang T, Zhao C, Wang D, Zhang X, Zhang H, Chi M, Yin S, Jia Y Tags: Gene Source Type: research

RNA sequencing reveals target genes of temporomandibular joint osteoarthritis in rats after the treatment of low-intensity pulsed ultrasound.
CONCLUSION: This study reveals the potential therapeutic genes related to TMJ-OA. Especially the circadian Per-2 gene was detected up-regulated by the treatment of LIPUS. It provides us a precious, new target OA-related gene and further investigation of gene-function will provide us new insights in understanding the potential mechanical underling TMJ-OA. PMID: 29885465 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 6, 2018 Category: Genetics & Stem Cells Authors: He D, An Y, Li Y, Wang J, Wu G, Chen L, Zhu G Tags: Gene Source Type: research

Comparative analysis on liver transcriptome profiles of different methods to establish type 2 diabetes mellitus models in Guangxi Bama mini-pig.
CONCLUSION: STZ can rapidly induce T2DM model in GBM pig, but STZ caused abnormal expression of genes in the liver. PMID: 29885466 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 6, 2018 Category: Genetics & Stem Cells Authors: Wu Y, Zhang L, Liang J, Jiang Q, Guo Y, Lan G Tags: Gene Source Type: research

Data integration for functional annotation of regulatory single nucleotide polymorphisms associated with Alzheimer's disease susceptibility.
Abstract BACKGROUND: Alzheimer's disease (AD), the most common form of dementia affects 24.3 million people worldwide. More than twenty genetic loci have been associated with AD and a significant number of genetic variants were mapped within these loci. A large proportion of genome wide significant variants lie outside the coding region. However, the plausible function of these variants is still unexplored. OBJECTIVE: The present study aimed to unravel the regulatory role of proxy single nucleotide polymorphisms (SNPs), to determine their risk of developing AD. METHODS: The RegulomeDB was employed to pre...
Source: Gene - June 5, 2018 Category: Genetics & Stem Cells Authors: Amber S, Zahid S Tags: Gene Source Type: research

Association of APOB and LIPC polymorphisms with type 2 diabetes in Chinese Han population.
CONCLUSION: This study suggests that the APOB polymorphism rs679899 is associated with type 2 diabetes and GGT levels, while the LIPC polymorphism rs6083 may influence CHOL, TG, and LDL levels in Chinese Han population. PMID: 29883758 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 5, 2018 Category: Genetics & Stem Cells Authors: Long T, Lu S, Li H, Lin R, Qin Y, Li L, Chen L, Zhang L, Lv Y, Liang D, Liang Y, Xie L, Yang H, Dong C, Zhang H Tags: Gene Source Type: research

SCUBE2 inhibits the proliferation, migration and invasion of human non-small cell lung cancer cells through regulation of the sonic hedgehog signaling pathway.
Abstract Signal peptide-CUB-EGF domain-containing protein 2 (SCUBE2) belongs to a secreted and membrane-associated multi-domain SCUBE protein family. Recently, it was found to play an important role in tumor development. However, the role of SCUBE2 in non-small cell lung cancer (NSCLC) has never been revealed. Here, we explored the roles and mechanism of SCUBE2 in the progression of NSCLC. Our result showed that SCUBE2 was expressed in low levels in NSCLC cell lines. In addition, overexpression of SCUBE2 significantly inhibited the proliferation, migration and invasion of NSCLC cells. Further mechanistic analysis ...
Source: Gene - June 5, 2018 Category: Genetics & Stem Cells Authors: Yang B, Miao S, Li Y Tags: Gene Source Type: research

Collagenase-1 (-1607 1G/2G), Gelatinase-A (-1306 C/T), Stromelysin-1 (-1171 5A/6A) functional promoter polymorphisms in risk prediction of type 2 diabetic nephropathy.
Abstract Type 2 Diabetic Nephropathy (DN) is a common multifactorial disorder. Degradation of glomerular basement membrane (GBM) by matrix metalloproteinases (MMPs) is a key event in the progression of renal disease. A functional polymorphism at position -1607 1G/2G, -1306 C/T and -1171 5A/6A has been shown to alter the transcriptional activity of MMP-1, MMP-2 and MMP-3 respectively, and also associated with several diseases contributing to inter-individual differences in susceptibility to type 2 DN. The study population consisted of 310 type 2 DN patients and 310 healthy controls. Genotypes of MMP-1, 2 and 3 were...
Source: Gene - June 5, 2018 Category: Genetics & Stem Cells Authors: Gantala SR, Kondapalli MS, Kummari R, Padala C, Tupurani MA, Kupsal K, Galimudi RK, Gundapaneni KK, Puranam K, Shyamala N, Guditi S, Rapur R, Hanumanth SR Tags: Gene Source Type: research

Generation of a recombinant Aggregatibacter actinomycetemcomitans RTX toxin in Escherichia coli.
Abstract A leukotoxin (LtxA) that is produced by Aggregatibacter actinomycetemcomitans (Aa) is an important virulence determinant in an aggressive form of periodontitis in adolescents. Understanding the function of this protein at the molecular level is critical to elucidating its role in the disease process. To accomplish genetic analysis of the protein structure and relating these observations to toxin function, we have developed an E. coli expression system for the generation and rapid purification of LtxA. Cloning the structural toxin gene, ltxA, from Aa strain JP2 under control of T7 promoter-1 of pCDFDuet-1 ...
Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Balashova N, Giannakakis A, Brown AC, Koufos E, Benz R, Arakawa T, Tang HY, Lally ET Tags: Gene Source Type: research

MicroRNA profiling of second trimester maternal plasma shows upregulation of miR-195-5p in patients with gestational diabetes.
This study aimed to determine the plasma miRNA expression profiles of the pregnant women with GDM and compare them to those of pregnant controls using the real-time PCR array method. The study involved 22 single-pregnancy women (mean age ± standard deviation of 29.9 ± 4.5 years old) who underwent a glucose tolerance test between 23 and 31 weeks of gestation. Of them, 13 were diagnosed with GDM. We identified 15 upregulated miRNAs in the GDM patients that were involved in 41 pathways. Among the top 10 associated pathways, fatty acid biosynthesis and fatty acid metabolism were targeted by the most, ...
Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Tagoma A, Alnek K, Kirss A, Uibo R, Haller-Kikkatalo K Tags: Gene Source Type: research

Isolation and characterization of porcine PILRB gene and its alternative splicing variants.
In this study, we obtained the cDNA (V1) of its encoding gene, PILRB, and three alternative splicing (AS) variants (V2-4). The complete coding sequence of V1 was 621 bp long encoding a polypeptide of 206 aa. Compared with V1, V2 and V3 were formed by exon-skipping in the 3'-untranslated region (UTR), while V4 was formed by alternative 3' splice site of exon 3, resulting in a premature termination codon, combined with exon skipping in the 3'-UTR. Expression profile analysis showed that all the isoforms were most abundant in the spleen, and V1 was strongly induced by poly(I:C). Furthermore, the transcription of V1 altered ...
Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Yang XQ, Jing XY, Zhang CX, Song YF, Liu D Tags: Gene Source Type: research

Digital gene-expression profiling analysis of the fatty liver of Landes geese fed different supplemental oils.
This study was designed to investigate the effect of different lipid sources on hepatic lipogenesis in overfed geese. Sixty Landes geese were fed ad libitum with no fat (control) or overfed diets containing 2% goose fat (GF) or rapeseed oil (RO) for 20 days. We measured fatty acid composition of the liver at day 20 of overfeeding. We performed a transcriptomic comparison of fatty liver between GF and RO-fed geese to gain insights into the molecular and cellular events mediating lipogenesis activity. The results showed that there was no substantial effect on fatty liver performance between GF- and RO-fed geese. Significan...
Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Tang J, Fang Q, Shao R, Shen J, He J, Niu D, Lu L Tags: Gene Source Type: research

The reciprocal link between EVI1 and miRNAs in human malignancies.
Abstract Ecotropic virus integration site-1 (EVI1) is an oncogenic transcription factor which locus on chromosome 3(3q26.2). Alterations in EVI1 functions correspond with poor prognosis in different cancers, underscoring their status for the clinical cancer phenotype. MicroRNAs(MiR)are a class of small non-coding RNA sequences. They post-transcriptionally influence mRNA sequence through imperfect pairing with the 3'-UTR. Moreover, a growing body of studies showed that miRNAs could regulate initiation and progression of human malignancies. Current studies have been described that identifies numerous microRNAs that ...
Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Lang WJ, Chen FY Tags: Gene Source Type: research

The overlap between regeneration and fibrosis in injured skeletal muscle is regulated by phosphatidylinositol 3-kinase/Akt signaling pathway - A bioinformatic analysis based on lncRNA microarray.
Abstract Injured skeletal muscle would go through a sequence of the pathological phases of degeneration, myogenesis and fibrosis. Growing evidence indicated that fibrotic and myogenic phases might overlap within the injured skeletal muscle in the early time after injury. However, the mechanism underlying this overlapping remains unclear. Here, we performed an lncRNA microarray to identify the activated pathways in mice muscle seven days after contusion. KEGG analysis indicated that phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signaling cascade was predicted to be activated by lncRNAs. The top genes targeted by lnc...
Source: Gene - June 2, 2018 Category: Genetics & Stem Cells Authors: Sun Y, Sun X, Liu S, Liu L, Chen J Tags: Gene Source Type: research

Synergistic defects of novo FAS and homozygous UNC13D leading to autoimmune lymphoproliferative syndrome-like disease: A 10-year-old Chinese boy case report.
Abstract Autoimmune lymphoproliferative syndrome (ALPS) usually presents in childhood with fever, nonmalignant splenomegaly and lymphadenopathy along with hemocytopenia. This case report describes a 10-year-old boy presenting with signs of autoimmune disease, splenomegaly, hepatomegaly and resistant hemocytopenia. Sirolimus controlled the relapsed thrombocytopenia after splenectomy. Sequencing of the FAS gene identified two spontaneous heterozygous mutations (c.234 T > G, p.D78E) (c.236dupA, p.P80Tfs*26). The boy's homozygous missense variation (c.2588G > A, p.G863D) (rs140184929) in UNC13D gene ha...
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: Gu H, Ma J, Chen Z, Wang J, Zhang R, Wu R Tags: Gene Source Type: research

The detection and significance of cystic fibrosis transmembrane conductance regulator gene promoter mutations in Chinese patients with congenital bilateral absence of the vas deferens.
CONCLUSION: Mutations in the promoter region of the CFTR gene in Chinese CBAVD patients are different from those found in comparable Caucasian patients. The homozygous c.-966 T > G mutation state had the highest frequency, which reduced the CFTR transcriptional level and showed significant tissue-specificity. PMID: 29864494 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: Bai S, Du Q, Liu X, Tong Y, Wu B Tags: Gene Source Type: research

A novel indel within goat casein alpha S1 gene is significantly associated with litter size.
This study aimed to detect possible novel indels within CSNs in an indigenous Chinese goat breed-Shaanbei White Cashmere goat (SBWC, n = 3047) and four other Chinese goat breeds (n = 1136) with varied litter size rates (105%-283%) and different estrus types (seasonal vs. perennial), as well as exploring the association between these potential indels and litter size. Only one novel 11-bp indel within the CSN1S1 gene was found. The association analyses uncovered that this novel indel was related to the first-birth litter size of SBWC population (n = 2690) (P 
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: Wang K, Yan H, Xu H, Yang Q, Zhang S, Pan C, Chen H, Zhu H, Liu J, Qu L, Lan X Tags: Gene Source Type: research

Overexpression of farnesyl pyrophosphate synthase increases myocardial ischemia/reperfusion injury in mice.
Abstract Farnesyl pyrophosphate synthase (FPPS) is a vital enzyme in the mevalonate pathway. Our previous study has indicated that overexpression of FPPS increases hypoxia/reoxygenation (HR) injury in Heart-derived H9c2 Cells. Hence, we designed this experiment to further investigate the effect of FPPS on myocardial ischemia/reperfusion (MIR) injury using a transgenic (Tg) model, and explore the relevant mechanisms. The results showed that when mouse hearts were subjected to ex vivo I/R, Tg mice have a higher CK and LDH, a larger myocardial infarct size and lower heart function recovery. These phenomena are associ...
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: Dai D, Sun X, Ding J, Chen Y, Hu S Tags: Gene Source Type: research

Differential expression of multiple glutamine synthetase genes in air-breathing magur catfish, Clarias magur and their induction under hyper-ammonia stress.
Abstract The present study demonstrates the unique presence of three different gs genes (cmgs01, cmgs02, and cmgs03) in air-breathing ureogenic magur catfish (Clarias magur), which is otherwise reported to be encoded by a single gene in higher vertebrates. Of these three genes, two (cmgs01and cmgs03) were identified as 'liver' form, predominantly expressed in liver cells, and the third one as 'brain' form (cmgs02), expressed chiefly in brain cells. Molecular characterization studies have revealed conservation of homologous active site residues in all the three gs genes. In silico analysis, accompanied by GS enzyme...
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: Banerjee B, Koner D, Bhuyan G, Saha N Tags: Gene Source Type: research

Inorganic arsenic exposure increased expression of Fas and Bax gene in vivo and vitro.
In this study, we investigated the association between levels of Fas and Bax expression and the three arsenic species (inorganic arsenic (iAs), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA)) in vivo and vitro. Three arsenic species in urine were measured and levels of Fas and Bax expression were examined by the quantitative real-time PCR (qPCR) for all subjects. We found that Fas and Bax mRNA expression in the exposed group were significantly higher than that in the control group. The levels of gene expression were positively correlated with the concentrations of urinary iAs, MMA and DMA in all subjects. Sodi...
Source: Gene - June 1, 2018 Category: Genetics & Stem Cells Authors: He Y, Zhang R, Xiaoxiao S, Li S, Xinan W, Huang D Tags: Gene Source Type: research

Association of interleukin-6 (rs1800796) but not transforming growth factor beta 1 (rs1800469) with serum calcium levels in osteoporotic patients.
CONCLUSION: IL-6 genotype influences serum calcium levels in osteoporotic patients. The lack of association between the common genetic variations of TGF-β1 and IL-6 genes, and BMD highlights the complex genetic background of osteoporosis in the north of Iran. PMID: 29860063 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - May 31, 2018 Category: Genetics & Stem Cells Authors: Eftekhari H, Hosseini SR, Baboli HP, Golchin MM, Heidari L, Abedian Z, Pourbagher R, Amjadi-Moheb F, Kani SNM, Nooreddini H, Akhavan-Niaki H Tags: Gene Source Type: research

Expression analysis of the MCPH1/BRIT1 and BRCA1 tumor suppressor genes and telomerase splice variants in epithelial ovarian cancer.
CONCLUSIONS: These findings support a regulatory effect of MCPH1/BRIT1 and BRCA1 on telomerase activity, particularly the negative association between MCPH1/BRIT1 and the functional form of hTERT (α+/β+). PMID: 29860064 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - May 31, 2018 Category: Genetics & Stem Cells Authors: Alsiary R, Brownhill SC, Brüning-Richardson A, Hutson R, Griffin N, Morrison EE, Bond J, Burchill SA, Bell SM Tags: Gene Source Type: research

PCL/PVA nanofibrous scaffold improve insulin-producing cells generation from human induced pluripotent stem cells.
In conclusion, this research could provide a new approach to beta-like cells replacement therapies and pancreatic tissue engineering for T1DM in the future. PMID: 29860065 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - May 31, 2018 Category: Genetics & Stem Cells Authors: Abazari MF, Soleimanifar F, Aleagha MN, Torabinejad S, Nasiri N, Khamisipour G, Mahabadi JA, Mahboudi H, Enderami SE, Saburi E, Hashemi J, Kehtari M Tags: Gene Source Type: research

Mutational analysis in patients with Autosomal Dominant Polycystic Kidney Disease (ADPKD): Identification of five mutations in the PKD1 gene.
In this study, we aimed to search for molecular causative defects among PKD1 and PKD2 genes. Eighteen patients were diagnosed based on renal ultrasonography and renal/extra-renal manifestations. Then, Sanger sequencing was performed for PKD1 and PKD2 genes. Multiplex Ligation dependent Probe Amplification method (MLPA) methods was performed for both PKD genes. Mutational analysis of the PKD2 gene revealed the absence of variants and no deletions or duplications of both PKD genes were detected. But three novels mutations i.e. p.S463C exon 7; c. c.11156+2T>C IVS38 and c.8161-1G>A IVS22 and two previously reported c.152...
Source: Gene - May 31, 2018 Category: Genetics & Stem Cells Authors: Abdelwahed M, Hilbert P, Ahmed A, Mahfoudh H, Bouomrani S, Dey M, Hachicha J, Kamoun H, Keskes-Ammar L, Belguith N Tags: Gene Source Type: research

Identification of a novel antisense long non-coding RNA PLA2G16-AS that regulates the expression of PLA2G16 in pigs.
Abstract Natural antisense transcripts (NATs) are widely present in mammalian genomes and act as pivotal regulator molecules to control gene expression. However, studies on the NATs of pigs are relatively rare. Here, we identified a novel antisense transcript, designated PLA2G16-AS, transcribed from the phospholipase A2 group XVI locus (PLA2G16) in the porcine genome, which is a well-known regulatory molecule of fat deposition. PLA2G16-AS and PLA2G16 were dominantly expressed in porcine adipose tissue, and were differentially expressed between Tibetan pigs and Rongchang pigs. In addition, PLA2G16-AS has a weak seq...
Source: Gene - May 31, 2018 Category: Genetics & Stem Cells Authors: Liu P, Jin L, Zhao L, Long K, Song Y, Tang Q, Ma J, Wang X, Tang G, Jiang Y, Zhu L, Li X, Li M Tags: Gene Source Type: research

Clinical variables and ethnicity may influenced by polymorphism of CAT -262C/T and MnSOD 47C/T antioxidant enzymes in Algerian type1 diabetes without complications.
tal N Abstract The latest studies in Algeria show that the frequency of type 1 diabetes (T1D) without complications is lower than that with complications and represents a significant burden in terms of cost and treatment. For this reason, we are interested in uncomplicated type1 diabetes and risk factors that are related to polymorphisms of antioxidant enzymes in order to prevent its complications. A total of 260 blood samples of young Algerian adults were examined. The genotypic analysis of Catalase gene (CAT -262C/T, rs1001179) and the superoxide dismutase gene (MnSOD 47C/T, rs4880) was performed by real-time PC...
Source: Gene - May 30, 2018 Category: Genetics & Stem Cells Authors: Eddaikra A, Amroun H, Raache R, Galleze A, Abdallah-Elhadj N, Azzouz M, Meçabih F, Mechti B, Abbadi MC, Touil-Boukoffa C, Attal N Tags: Gene Source Type: research

MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx.
This study was designed to examine whether the miR-132 regulate other proteins in OB during olfactory learning. To test this, miR-132 anti-sense oligodeoxynucleotide (AS-ODN) was delivered to the OB and then trained to novel odor. The 2-dimensional gel electrophoresis analysis showed that inhibition of miR-132 altered olfactory training induced expression of 321 proteins. Further, liquid chromatography-mass spectrometry (LC-MS/MS) analysis reveals the identity of differently expressed proteins such as phosphoribosyl transferase domain containing protein (PRTFDC 1), Sorting nexin-8 (SNX8), Creatine kinase B-type (CKB) and A...
Source: Gene - May 30, 2018 Category: Genetics & Stem Cells Authors: Mukilan M, Rajathei DM, Jeyaraj E, Kayalvizhi N, Rajan KE Tags: Gene Source Type: research