Comparative analysis of mitochondrial genomes in Ceratocystis fimbriata complex across diverse hosts
This study was aimed to investigate the mitochondrial genome architecture of two differentC. fimbriatacomplex isolates from Malaysia: one fromA. mangiumin Pahang (FRIM1162) and another fromEucalyptus pellitain Sarawak (FRIM1441). This research employed Next- Generation Sequencing (NGS) to contrast genomes from diverse hosts with nine additional mitochondrial sequences, identifying significant genetic diversity and mutational hotspots in the mitochondrial genome alignment. The mitochondrial genome-based phylogenetic analysis revealed a significant genetic relationship between the studied isolates and theC. fimbriatacomplex ...
Source: Gene - May 6, 2024 Category: Genetics & Stem Cells Authors: Samsuddin Ahmad Syazwan Ahmad Mohd-Farid Shiou Yih Lee Rozi Mohamed Source Type: research
In vitro study of HPV18-positive cervical cancer HeLa cells based on CRISPR/Cas13a system
Gene. 2024 May 4:148527. doi: 10.1016/j.gene.2024.148527. Online ahead of print.ABSTRACTThe E6 protein is a known oncogene in cervical cancer and plays a key role in the development and progression of cervical cancer by reducing the expression level of the tumor suppressor protein P53 and ultimately leading to enhanced cell proliferation and reduced apoptosis. Therefore, antiviral agents that inhibit the expression of E6 oncoprotein are expected to be potential therapies for human cervical cancer. Here we developed CRISPR/Cas13a: crRNA dual plasmid system and demonstrated that CRISPR/Cas13a could effectively and specifical...
Source: Gene - May 6, 2024 Category: Genetics & Stem Cells Authors: Anran Zhang Xue Zheng Shuaiyin Chen Guangcai Duan Source Type: research
Fengshi Liuhe Decoction treatment for rheumatoid arthritis via the Fzd6/NF- κB signaling axis
Gene. 2024 May 4:148538. doi: 10.1016/j.gene.2024.148538. Online ahead of print.ABSTRACTTo explore whether Fengshi Liuhe Decoction (FLD) alleviates rheumatoid arthritis (RA) via the Fzd6/NF-κB signaling axis. We used real-time quantitative PCR (qPCR) and western blotting (WB) to determine the genes of the frizzled (Fzd) protein 1- Fzd protein 10 that are significantly differentially expressed between normal rat f fibroblast-like synoviocyte (FLS) and collagen II-induced arthritis (CIA) rat FLS. Next, we used enzyme-linked immunosorbent assay (ELISA) to evaluate the levels of inflammatory factors in cell culture supernatan...
Source: Gene - May 6, 2024 Category: Genetics & Stem Cells Authors: Fulin Zhou Peilin Zhou Xian Jiang Zhicheng Yang Weiding Cui Weixi Liu Ruiping Liu Source Type: research
Identification of cotton PIP5K genes and role of GhPIP5K9a in primary root development
This study identified PIP5K genes from four cotton species and conducted bioinformatic analyses, with a particular emphasis on the functions of GhPIP5K9a in primary roots. The results showed that cotton PIP5Ks were classified into four subgroups. Analysis of gene structure and motif composition showed obvious conservation within each subgroup. Synteny analysis suggested that the PIP5K gene family experienced significant expansion due to both whole-genome duplication (WGD) and segmental duplication. Transcriptomic data analysis revealed that the majority of GhPIP5K genes had the either low or undetectable levels of expressi...
Source: Gene - May 5, 2024 Category: Genetics & Stem Cells Authors: Kaikai Qiao Jiaoyan Lv Juxin Hao Chenglong Zhao Shuli Fan Qifeng Ma Source Type: research
Different expression patterns of DNA methyltransferases during horse testis development
Gene. 2024 May 4;920:148531. doi: 10.1016/j.gene.2024.148531. Online ahead of print.ABSTRACTDNA methyltransferases (DNMTs) are important epigenetic modification during spermatogenesis. To further evaluate the pattern of DNMTs in horse testes during development, we investigated the expression and localization of DNMT1, DNMT3a and DNMT3b at different time points. The qRT-PCR results showed that DNMT1 expression was maintained in testes tissue from 6-month-old (0.5y) to 2-year-old (2y) of age and decreased after 3-year-old (3y) (P < 0.01). The expression levels of DNMT3a and DNMT3b peaked in testes tissue at 3y (P < 0.0...
Source: Gene - May 5, 2024 Category: Genetics & Stem Cells Authors: Minna Yi Nairag Asgenbaatar Xisheng Wang Tseweendolmaa Ulaangerel Yingchao Shen Xin Wen Ming Du Xiaoling Dong Manglai Dugarjav Gerelchimeg Bou Source Type: research
Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei
In this study, we identified a sequence of the MyoD gene (LvMyoD) with a conserved bHLH domain in the L. vannamei genome. Phylogenetic analysis revealed that both the overall protein sequence and specific functional sites of LvMyoD are highly conserved with those of other crustacean species and that they are evolutionarily closely related to vertebrate MyoD and Myf5. LvMyoD expression is initially high during early muscle development in shrimp and gradually decreases after 40 days post-larval development. In adults, the muscle-specific expression of LvMyoD was confirmed through RT-qPCR analysis. Knockdown of LvMyoD inhibit...
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Yanting Xia Xiaojun Zhang Xiaoxi Zhang Haochen Zhu Xiaoyun Zhong Weixiao Song Jianbo Yuan Zhenxia Sha Fuhua Li Source Type: research
The expression of AcIDI1 reveals diterpenoid Alkaloids' allocation strategies in the roots of Aconitum carmichaelii Debx
This study establishes a foundation for applying the IDI gene to regulate the biosynthesis and accumulation of DAs in A. carmichaelii.PMID:38703864 | DOI:10.1016/j.gene.2024.148529 (Source: Gene)
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Yiwen Hu Lijuan Chen Li Huang Guangzhi Wang Source Type: research
Selection of a suitable reference gene for gene-expression studies in Trichomonas gallinae under various biotic and abiotic stress conditions
This study quantified the transcript levels of ten candidate reference genes in T. gallinae isolates with different genotypes and culture conditions using qPCR. Using the geNorm, NormFinder, and BestKeeper algorithms, we assessed these reference genes' stabilities and ranked them using RankAggreg analysis. The most stable reference gene was tubulin beta chain (TUBB), while the widely used reference genes TUBG and GAPDH demonstrated poor stability. Additionally, we evaluated these candidate reference genes' stabilities using the T. gallinae TgaAtg8 gene. On using TUBB as a reference gene, TgaAtg8's expression profiles in T....
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Haiming Cai Yibin Zhu Yu Liu Zhuanqiang Yan Hanqin Shen Siyun Fang Dingai Wang Shenquan Liao Juan Li Minna Lv Xuhui Lin Junjing Hu Yongle Song Xiangjie Chen Lijun Yin Jianfei Zhang Nanshan Qi Mingfei Sun Source Type: research
Molecular characterization and phylogenetic analysis of babA gene of Helicobacter pylori isolated from Indian patients with gastrointestinal diseases
CONCLUSION: The appearance of variants within distinct disease categories is due to local genetic variation.PMID:38703866 | DOI:10.1016/j.gene.2024.148526 (Source: Gene)
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Sarika Singh Amresh Kumar Sharma Anup Som Valentina Gehlot Shweta Mahant Prateek Sharma Kunal Das Rajashree Das Source Type: research
Identification of LPCAT1 as a key biomarker for Crohn's disease based on bioinformatics and machine learnings and experimental verification
This study aims to explore the molecular mechanisms of EMT-related genes in Crohn's disease (CD) through bioinformatics methods and identify potential key biomarkers. In our research, we identified differentially expressed genes (DEGs) related to EMT based on the GSE52746 dataset and the gene set in the GeneCards database. Key genes were identified through Lasso-cox and Random Forest and validated using the external dataset GSE10616. Immune infiltration analysis showed that Lysophosphatidylcholine acyltransferase 1 (LPCAT1) was positively correlated with Neutrophils and Macrophages M1. The Gene Set Enrichment Analysis (GSE...
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Wei Chen Zeyan Xu Jingjing Jiang Hong Chen Ruihua Shi Source Type: research
Potential global cis and trans regulation of lncRNAs in Saccharomyces cerevisiae subjected to ethanol stress
Gene. 2024 May 3;920:148521. doi: 10.1016/j.gene.2024.148521. Online ahead of print.ABSTRACTLong noncoding RNAs (lncRNAs) are regulatory RNAs. Saccharomyces cerevisiae strains transcribe hundreds of lncRNAs. LncRNAs can regulate the expression of adjacent genes (cis-regulation) or distant genes from lncRNAs (trans-regulation). Here, we analyzed the potential global cis and trans-regulation of lncRNAs of yeast subjected to ethanol stress. For potential cis regulation, for BMA641-A and S288C strains, we observed that most lncRNA-neighbor gene pairs increased the expression at a certain point followed by a decrease, and vice ...
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Amanda Piveta Schnepper Lucas Farinazzo Marques Ivan Rodrigo Wolf Agatha M S Kubo Guilherme Targino Valente Source Type: research
Association of the E23K (rs5219) polymorphism in the potassium channel (KCNJ11) gene with diabetic neuropathy in type 2 diabetes
Gene. 2024 May 2:148525. doi: 10.1016/j.gene.2024.148525. Online ahead of print.NO ABSTRACTPMID:38703869 | DOI:10.1016/j.gene.2024.148525 (Source: Gene)
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Rasha Elzehery Hala Abd El-Hafez Ibrahim Elsehely Amira Barakat Engy Ahmed Ebrahim Foda Shimaa Rabea Hendawy Mohammed Ali Gameil Hyam Sameh Nada Ahmed El-Sebaie Source Type: research
The mechanism of Traditional Mongolian medicine Daruqi particles on inflammation
Gene. 2024 May 2:148530. doi: 10.1016/j.gene.2024.148530. Online ahead of print.ABSTRACTDaruqi is a Traditional Mongolian medicine with anti-inflammatory, anti-bacterial, and immune-regulatory effects. However, the mechanisms of its activity were unclear. In the present study, we confirmed the anti-inflammation effect of Daruqi on inflammation induced by LPS using animal models. Then, THP-1 cells treated with LPS was used as a positive control to explore the effective component of Daruqi on inflammation. We identified that Oxymatrine was the essential effector of Daruqi. Furthermore, the mechanism of Oxymatrine on inflamma...
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Zhi Liu Zhiqiang Han Lan Xue Wei Wei None Batudeligen Source Type: research
Transcriptomic analysis of epicardial adipose tissue reveals the potential crosstalk genes and immune relationship between type 2 diabetes mellitus and atrial fibrillation
CONCLUSION: This investigation reveals the molecular links between T2DM and AF with a focus on EAT. Targeting these pathways, especially EAT-related ones, may enable personalized treatments and improved outcomes.PMID:38703871 | DOI:10.1016/j.gene.2024.148528 (Source: Gene)
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Tian-Lun Li Na-Na Zhu Zhao Yin Jiao Sun Jian-Pin Guo Hong-Tao Yuan Xiang-Min Shi Hong-Yang Guo Shi-Xing Li Zhao-Liang Shan Source Type: research
Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei
In this study, we identified a sequence of the MyoD gene (LvMyoD) with a conserved bHLH domain in the L. vannamei genome. Phylogenetic analysis revealed that both the overall protein sequence and specific functional sites of LvMyoD are highly conserved with those of other crustacean species and that they are evolutionarily closely related to vertebrate MyoD and Myf5. LvMyoD expression is initially high during early muscle development in shrimp and gradually decreases after 40 days post-larval development. In adults, the muscle-specific expression of LvMyoD was confirmed through RT-qPCR analysis. Knockdown of LvMyoD inhibit...
Source: Gene - May 4, 2024 Category: Genetics & Stem Cells Authors: Yanting Xia Xiaojun Zhang Xiaoxi Zhang Haochen Zhu Xiaoyun Zhong Weixiao Song Jianbo Yuan Zhenxia Sha Fuhua Li Source Type: research
