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Insights into the multifunctional role of natural killer enhancing factor-A (NKEF-A/Prx1) in big-belly seahorse (Hippocampus abdominalis): DNA protection, insulin reduction, H2O2 scavenging, and immune modulation activity.
This study provides insight into the functions of NKEF-A/Prx1 in fish species. PMID: 29155258 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Godahewa GI, Perera NCN, Lee J Tags: Gene Source Type: research

Promoter activity and regulation of the Pou5f1 homolog from a teleost, Nile tilapia.
Abstract Mammalian POU5F1 (also known as OCT4) is an essential transcription factor that induces and controls stemness in the inner cell mass and embryonic stem (ES) cells. Its expression results from intricate regulatory networks involving its 5' upstream DNA elements and numerous transcription factors. Pou5f3, the ortholog of POU5F1, has been identified in non-mammalians including fish. However, little is known about the molecular mechanisms controlling its expression up to date. Here we report the promoter activity and regulation of Nile tilapia (Oreochromis niloticus) pou5f3 (Onpou5f3) in fish early-stage embr...
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Jing W, Xiaohuan H, Zhenhua F, Zhuo Y, Fan D, Wenjing T, Linyan Z, Deshou W Tags: Gene Source Type: research

Genome-wide identification of the SWEET gene family in wheat.
In conclusion, we provide the first comprehensive analysis of the wheat SWEET gene family. Information regarding the phylogenetic relationships, gene structures, and expression profiles of SWEET genes in different tissues and following stem rust disease inoculation will be useful in identifying the potential roles of SWEETs in specific developmental and pathogenic processes. PMID: 29155326 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Gao Y, Wang ZY, Kumar V, Xu XF, Yuan P, Zhu XF, Li TY, Jia BL, Xuan YH Tags: Gene Source Type: research

Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp Fenneropenaeus chinensis in response to viral and bacterial infections.
Abstract Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, However, compared with mammals and insects, the potential function of p38 MAPK from Chinese shrimp (Fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. In the study, the immune responses of four genes MKK3, MKK4, p38 and ATF-2 from F. chinensis were investigated in defending against white spot syndrome virus (WSSV), Vibrio anguillarum (V. anguillarum) and Staphylococcus aureus (S. aureus) infection....
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: He Y, Yao W, Liu P, Li J, Wang Q Tags: Gene Source Type: research

Mitochondrial tRNA(Leu(UUR)) C3275T, tRNA(Gln) T4363C and tRNA(Lys) A8343G mutations may be associated with PCOS and metabolic syndrome.
Abstract Polycystic ovary syndrome (PCOS) is a very prevalent endocrine disease affecting reproductive women. Clinically, patients with this disorder are more vulnerable to develop type 2 diabetes mellitus (T2DM), cardiovascular events, as well as metabolic syndrome (MetS). To date, the molecular mechanism underlying PCOS remains largely unknown. Previously, we showed that mitochondrial dysfunction caused by mitochondrial DNA (mtDNA) mutation was an important cause for PCOS. In the current study, we described the clinical and biochemical features of a three-generation pedigree with maternally transmitted MetS, com...
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Ding Y, Xia BH, Zhang CJ, Zhuo GC Tags: Gene Source Type: research

Cloning and characterization of two novel β-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea.
Cloning and characterization of two novel β-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea. Gene. 2017 Nov 16;: Authors: Chan AKN, Ng AKL, Ng KKY, Wong WKR Abstract Enzymatic degradation of cellulosic waste to generate renewable biofuels has offered an attractive solution to the energy problem. Synergistic hydrolysis of cellulose residues requires the participation of three different types of cellulases - endoglucanases, exoglucanases, and β-glucosidases (Bgl). Our group has been interested in using Bgl of Cellulomonas biazotea in studies designed ...
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Chan AKN, Ng AKL, Ng KKY, Wong WKR Tags: Gene Source Type: research

Expression pattern and functional analysis of R-spondin1 in tongue sole Cynoglossus semilaevis.
This study laid the groundwork for further sex control breeding techniques in C. semilaevis. PMID: 29155330 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Liu J, Liu T, Niu J, Wu X, Zhai J, Zhang Q, Qi J Tags: Gene Source Type: research

Evolution of vomeronasal receptor 1 (V1R) genes in the common marmoset (Callithrix jacchus).
Abstract Pheromones are crucial for eliciting innate responses and inducing social and sexual behaviors in mammals. The vomeronasal receptor 1 genes, V1Rs, encode members of a pheromone receptor family that are mainly expressed in the vomeronasal organ (VNO). The V1R family shows extraordinary variety in gene number among vertebrates owing to successive gene gains and losses during evolution. Such diversity is believed to reflect a degree of dependence on the VNO. We investigated V1R evolution in primate lineages closely related to humans because these VNOs show a trend toward degeneration. We performed extensive ...
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Moriya-Ito K, Hayakawa T, Suzuki H, Hagino-Yamagishi K, Nikaido M Tags: Gene Source Type: research

Unusual features of the large linear plasmid pSA3239 from Streptomyces aureofaciens CCM 3239.
We report here the nucleotide sequence of this 241,076-bp plasmid. pSA3239 contains an unprecedentedly small (13bp) telomeric sequence CCCGCGGAGCGGG, which is identical to the conserved Palindrome I sequence involved in the priming of end-patching replication. A bioinformatics analysis revealed 234 open reading frames with high number (28) of regulatory genes from various families. In contrast to most other linear plasmids, pSA3239 contains a pair of replication initiation genes (sa76 and sa75) located at its extreme left end, adjacent to the telomere. Together with similar proteins from several other linear plasmids (pFRL...
Source: Gene - November 16, 2017 Category: Genetics & Stem Cells Authors: Mingyar E, Novakova R, Knirschova R, Feckova L, Bekeova C, Kormanec J Tags: Gene Source Type: research

Contrasting the microbiomes from forest rhizosphere and deeper bulk soil from an Amazon rainforest reserve.
Abstract Pristine forest ecosystems provide a unique perspective for the study of plant-associated microbiota since they host a great microbial diversity. Although the Amazon forest is one of the hotspots of biodiversity around the world, few metagenomic studies described its microbial community diversity thus far. Understanding the environmental factors that can cause shifts in microbial profiles is key to improving soil health and biogeochemical cycles. Here we report a taxonomic and functional characterization of the microbiome from the rhizosphere of Brosimum guianense (Snakewood), a native tree, and bulk soil...
Source: Gene - November 15, 2017 Category: Genetics & Stem Cells Authors: Fonseca JP, Hoffmann L, Cabral BCA, Dias VHG, Miranda MR, de Azevedo Martins AC, Boschiero C, Bastos WR, Silva R Tags: Gene Source Type: research

Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells.
Abstract DNA binding domains (DBDs) have been used with great success to impart targeting capabilities to a variety of proteins creating highly useful genomic tools. We evaluated the ability of five types of DBDs and strategies (AAV Rep proteins, Cre, TAL effectors, zinc finger proteins, and Cas9/gRNA system) to target the L1 ORF2 protein to drive retrotransposition of Alu inserts to specific sequences in the human genome. First, we find that the L1 ORF2 protein tolerates the addition of protein domains both at the amino- and carboxy-terminus. Although in some instances retrotransposition efficiencies slightly dim...
Source: Gene - November 14, 2017 Category: Genetics & Stem Cells Authors: Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM Tags: Gene Source Type: research

The FTO variant is associated with chronic complications of diabetes mellitus in Czech population.
Abstract BACKGROUND: Genome-wide association studies have resulted in the identification of the FTO gene as an important genetic determinant of diabetes mellitus. The aim of this study was to confirm the role of this gene in the development of DM in the Czech-Slavonic population and to analyse whether this gene is associated with common DM complications. METHODS: Two groups of patients (814 with T1DM and 848 with T2DM) and a group of healthy controls (2339 individuals) - both of Czech origin - were genotyped for the FTO rs17817449 SNP. ANOVA and logistic regression were used for the statistical evaluations. ...
Source: Gene - November 14, 2017 Category: Genetics & Stem Cells Authors: Hubacek JA, Dlouha D, Klementova M, Lanska V, Neskudla T, Pelikanova T Tags: Gene Source Type: research

Association between genetic polymorphisms in the promoters of let-7 and risk of cervical squamous cell carcinoma.
Abstract Numerous reports showed low levels of let-7 family in cervical cancer, acting as tumor suppressors by regulating multiple target genes. Genetic variants in the promoter of miRNA have been reported to influence individuals' susceptibility to human diseases. We aimed to investigate the association of rs10877887 and rs13293512 polymorphisms in the promoters of let-7 with risk of cervical squamous cell carcinoma (CSCC). A total of 331 patients with CSCC and 358 controls were included. Genotyping of rs10877887 was done using polymerase chain reaction-restriction fragment length polymorphism analysis. Genotypin...
Source: Gene - November 14, 2017 Category: Genetics & Stem Cells Authors: Liu J, Ni S Tags: Gene Source Type: research

Starvation-, thermal- and heavy metal- associated expression of four small heat shock protein genes in Musca domestica.
In this study, starvation-, thermal- and heavy metal-associated expression of four small Musca domestica HSPs (abbreviated as MdomHSPs and they are MdomHSP10, MdomHSP27, MdomHSP27.1 and MdomHSP27.2) were determined. The following results were found: All MdomHSPs were significantly higher expressed during the active larval and adult stages than the egg and pupal stages; All MdomHSPs were expressed at relatively equal levels in the head, thorax and abdomen of adults; The expression of MdomHSP27 was significantly down-regulated in 4-day-old larvae that were starved for 6h, while the other 3 MdomHSPs were not significantly aff...
Source: Gene - November 14, 2017 Category: Genetics & Stem Cells Authors: Tian L, Wang X, Wang X, Lei C, Zhu F Tags: Gene Source Type: research

Medaka igf1 identifies somatic cells and meiotic germ cells of both sexes.
Abstract Insulin-like growth factors (IGFs) play significant roles in regulating cell proliferation, differentiation and reproduction in diverse organisms. Here, we report the identification of medaka igf1 and the expression pattern of igf1 RNA in adult medaka gonads. Using RT-PCR, igf1 RNA was easily detected in several somatic organs and adult gonads of both sexes. Using chromogenic in situ hybridization, igf1 RNA in the ovary was found in stage I-IV oocytes and in the somatic cells throughout oogenesis. In the testis, igf1 RNA was present in meiotic products and the somatic cells surrounding male germ cells thr...
Source: Gene - November 14, 2017 Category: Genetics & Stem Cells Authors: Yuan C, Chen K, Zhu Y, Yuan Y, Li M Tags: Gene Source Type: research

Association analyses of genetic variants in long non-coding RNA MALAT1 with breast cancer susceptibility and mRNA expression of MALAT1 in Chinese Han population.
Abstract The long non-coding RNA (lncRNA) Metastasis-associated lung adenocarcinoma transcript 1(MALAT1) has been implicated in breast cancer (BC). Polymorphisms in MALAT1 may play a vital role in the progress of breast cancer by its regulation function. However, potential genetic variants in MALAT1 affecting the development of BC is rarely explored. In our current molecular epidemiology study, all three tagging SNPs (rs3200401, rs619586 and rs7927113) in lncRNA MALAT1 were selected for genotyping in 487BCE patients and 489 cancer-free controls in Chinese Han population, and futher experiment of quantitative real-...
Source: Gene - November 13, 2017 Category: Genetics & Stem Cells Authors: Peng R, Luo C, Guo Q, Cao J, Yang Q, Dong K, Wang S, Wang K, Song C Tags: Gene Source Type: research

ASIC2a overexpression enhances the protective effect of PcTx1 and APETx2 against acidosis-induced articular chondrocyte apoptosis and cytotoxicity.
Abstract Acid hydrarthrosis is another important pathological character in rheumatoid arthritis (RA), and acid-sensing ion channel 1a (ASIC1a) plays a destructive role in acidosis-induced articular chondrocyte cytotoxicity. Recently, ASIC2a has been reported to possess neuroprotective effect on acidosis-induced injury of neuronal cells. However, whether ASIC2a has an enhanced effect on the protective effect of blocking ASIC1a and ASIC3 against acid-induced chondrocyte apoptosis is still unclear. The aim of present study was to investigate the chondroprotective effect of ASIC2a with PcTx1 (ASIC1a specific blocker) ...
Source: Gene - November 13, 2017 Category: Genetics & Stem Cells Authors: Zhou RP, Ni WL, Dai BB, Wu XS, Wang ZS, Xie YY, Wang ZQ, Yang WJ, Ge JF, Hu W, Chen FH Tags: Gene Source Type: research

EGFR and Ras regulate DDX59 during lung cancer development.
In this report, we found that both EGFR and Ras signaling can upregulate RNA helicase DDX59 in lung cancer cells. DDX59 can be induced through the mitogen activated protein kinase (MAPK) pathway after EGFR or Ras activation. Inhibitors for Ras/Raf/MAP pathway significantly decreased DDX59 expression at both protein and mRNA levels. Through immunohistochemistry, we found that DDX59 protein expression correlated with Ras and EGFR mutation status in human lung adenocarcinoma. Finally, through a xenograft nude mice model, we demonstrated that DDX59 is pivotal for EGFR mutated lung cancer cell growth in vivo. Our study identifi...
Source: Gene - November 10, 2017 Category: Genetics & Stem Cells Authors: Yang L, Zhang H, Chen D, Ding P, Yuan Y, Zhang Y Tags: Gene Source Type: research

Association of genetic polymorphisms with erythrocyte traits: Verification of SNPs reported in a previous GWAS in a Japanese population.
Abstract Erythrocyte count and volume are the commonly used hematological indices for anemia that change in various diseases. To date, however, only one study ever exists that addressed erythrocyte trait-associated single nucleotide polymorphisms (SNPs) in a Japanese population. Because that study was performed in patients with various diseases, we confirmed the reported associations in a general population. Participants in the current study were from the Shizuoka component of the Japan Multi-Institutional Collaborative Cohort Study, which included 4971 men and women aged 35 to 69years who were recruited between 2...
Source: Gene - November 10, 2017 Category: Genetics & Stem Cells Authors: Seiki T, Naito M, Hishida A, Takagi S, Matsunaga T, Sasakabe T, Hattori Y, Kawai S, Okada R, Yin G, Hamajima N, Wakai K Tags: Gene Source Type: research

cDNA sequences of GHF9 endo- β-1,4-glucanases in terrestrial Crustacea.
This study aimed to sequence and identify a glycosyl hydrolase family 9 (GHF9) endo-β-1,4-glucanase expressed in the midgut gland of the herbivorous gecarcinid land crab, Gecarcoidea natalis. Hence this would explain the gene responsible for the production of previously purified and characterised endo-β-1,4-glucanases. Three different transcripts, two complete and one partial were sequenced from cDNA and an open reading frame of 1383bp was produced. Translated, this would produce a putative protein of 460 amino acid residues, including a 16 amino acid residue signal peptide. The mature protein (without signal pep...
Source: Gene - November 10, 2017 Category: Genetics & Stem Cells Authors: Gray M, Linton SM, Allardyce BJ Tags: Gene Source Type: research

The long noncoding RNA expression profiles of paroxysmal atrial fibrillation identified by microarray analysis.
Abstract BACKGROUND: Long noncoding RNAs (lncRNAs) represent a novel class of noncoding RNAs that are involved in a variety of biological processes and human diseases. Recent evidence suggested that lncRNAs were associated with cardiac disorders. However, the roles of lncRNAs in paroxysmal atrial fibrillation (PAF) remain elusive. The purpose of the present study was to identify differentially expressed lncRNAs in PAF and predict their potential functions. METHODS: Between May 2014 and December 2015, a total of 67 patients, including 34 patients with PAF and 33 patients without PAF were recruited in this stud...
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Su Y, Li L, Zhao S, Yue Y, Yang S Tags: Gene Source Type: research

Overexpression of FOXA1 inhibits cell proliferation and EMT of human gastric cancer AGS cells.
In conclusion, these data suggests that FOXA1 may function as a novel anti-oncogene in gastric cancer cells. PMID: 29129808 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Lin M, Pan J, Chen Q, Xu Z, Lin X, Shi C Tags: Gene Source Type: research

Characterization and spatiotemporal expression analysis of nine bone morphogenetic protein family genes during intermuscular bone development in blunt snout bream.
In this study, we firstly obtained and characterized nine bmp genes for Megalobrama amblycephala, which belongs to Cyprinidae and have a certain amount of IBs. Sequence alignment and phylogenetic analysis both documented that the mature proteins of M. amblycephala bmp genes were highly conserved with other corresponding homologs, respectively, indicating that the function of each bmp gene has been conserved throughout evolution. As a step to characterize potential involvement of bmp genes in IB formation and development, spatiotemporal expressions of nine bmp genes (bmp2a, bmp2b, bmp3, bmp4, bmp5, bmp7b, bmp8a, bmp14 and b...
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Zhang WZ, Lan T, Nie CH, Guan NN, Gao ZX Tags: Gene Source Type: research

Discerning molecular interactions: A comprehensive review on biomolecular interaction databases and network analysis tools.
Abstract Computational analysis of biomolecular interaction networks is now gaining a lot of importance to understand the functions of novel genes/proteins. Gene interaction (GI) network analysis and protein-protein interaction (PPI) network analysis play a major role in predicting the functionality of interacting genes or proteins and gives an insight into the functional relationships and evolutionary conservation of interactions among the genes. An interaction network is a graphical representation of gene/protein interactome, where each gene/protein is a node, and interaction between gene/protein is an edge. In ...
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Miryala SK, Anbarasu A, Ramaiah S Tags: Gene Source Type: research

Transcriptome - Scale characterization of salt responsive bean TCP transcription.
This study identified 27 TCP genes of Phaseolus vulgaris (common bean), which were divided into three clusters based on phylogenetic relationship. In addition, this study showed that some of TCP genes such as Pvul-TCP-4 and Pvul-TCP-15 located on chromosomes 3 and 7, Pvul-TCP-7 and Pvul-TCP-20 located on chromosome 7 and 9, were segmentally duplicated. On the other hand, a total of 20 Pvul-TCP genes have predicted to be targeted by microRNAs (miRNA). Most of the miRNA-target genes were Pvul-TCP-1, -11, -13 and -27, which were targeted by 13, 17, 22 and 13 plant miRNAs, respectively. miR319 was one of the highly represented...
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: İlhan E, Büyük İ, İnal B Tags: Gene Source Type: research

Influence of LGALS3 gene polymorphisms on susceptibility and prognosis of dilated cardiomyopathy in a Northern Han Chinese population.
Conclusion, LGALS3 gene polymorphisms might be associated with the susceptibility of DCM in a Northern Han Chinese population. PMID: 29129812 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Zhang Y, Wang Y, Zhai M, Gan T, Zhao X, Zhang R, An T, Huang Y, Zhou Q, Zhang J Tags: Gene Source Type: research

A novel duplication downstream of BMP2 in a Chinese family with Brachydactyly type A2 (BDA2).
CONCLUSION: The novel duplication has different breakpoints compared with the previous ones but highly overlapped with them. The duplication narrows the range of the potential cis-regulatory sequence, and further supports the association between BDA2 and the duplication downstream BMP2. PMID: 29129813 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 9, 2017 Category: Genetics & Stem Cells Authors: Wang WB, Jia YC, Zhang Z, Xu J, Zuo RT, Kang QL Tags: Gene Source Type: research

Genome-wide mapping of estrogen receptor α binding sites by ChIP-seq to identify genes related to sexual maturity in hens.
This study also provides insight into the regulation of the ERα target gene networks and a reference for understanding ERα-regulated transcription. PMID: 29128632 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Guo M, Li Y, Chen Y, Guo X, Yuan Z, Jiang Y Tags: Gene Source Type: research

PHGDH is an independent prognosis marker and contributes cell proliferation, migration and invasion in human pancreatic cancer.
CONCLUSIONS: Our data indicated that the expression of PHGDH is increased in pancreatic cancer and is an independent molecular prognostic factor for pancreatic cancer patients. In addition, PHGDH controls cell proliferation, migration and invasion abilities. Therefore, PHGDH could serve as an important prognostic indicator and therapeutic target for pancreatic cancer. PMID: 29128633 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Song Z, Feng C, Lu Y, Lin Y, Dong C Tags: Gene Source Type: research

Vitamin D receptor (VDR) polymorphisms are associated to spontaneous preterm birth and maternal aspects.
In conclusion our findings indicate an association between polymorphisms FokI and Cdx-2 within VDR gene and SPTB, suggesting their involvement in the triggering of these syndromes. PMID: 29128634 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Javorski NR, Lima CAD, Silva LVC, Crovella S, de Azêvedo Silva J Tags: Gene Source Type: research

MicroRNA-374b reduces the proliferation and invasion of colon cancer cells by regulation of LRH-1/Wnt signaling.
Abstract Deregulation of microRNA (miRNA) has been suggested as a critical event in colon cancer development and progression. Recent studies have suggested that miR-374b is a novel cancer-related miRNA involved in several cancer types. Thus far, very little is known about the role of miR-374b in colon cancer; therefore, the goal of this study was to investigate the potential role of miR-374b in colon cancer. Here, we showed that miR-374b expression was significantly downregulated in colon cancer tissues and cell lines. Overexpression of miR-374b inhibited the proliferation and invasion of colon cancer cells, while...
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Qu R, Hao S, Jin X, Shi G, Yu Q, Tong X, Guo D Tags: Gene Source Type: research

Identification of key microRNAs associated with diffuse large B-cell lymphoma by analyzing serum microRNA expressions.
CONCLUSION: hsa-miR-34a-5p may be directly regulated by TP53 and may be involved in DLBCL development via the p53 signaling pathway. Furthermore, hsa-miR-323b-3p may be related to DLBCL by participating in pathways in cancer. hsa-miR-431-5p may also play a role in DLBCL by regulating FYN. PMID: 29128636 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Meng Y, Quan L, Liu A Tags: Gene Source Type: research

A MADS-box transcription factor of 'Kuerlexiangli'(Pyrus sinkiangensis Yu) PsJOINTLESS gene functions in floral organ abscission.
Abstract MADS-box proteins have been implicated in many biological processes. However, plant MADS-box proteins functioning in floral organ abscission and the underlying physiological mechanisms remain poorly understood. Here, we report the identification and functional characterization of PsJOINTLESS isolated from 'Kuerlexiangli'. PsJOINTLESS had a complete open reading frame of 672bp, encoding a 224 amino acid peptide, and shared high sequence identities with MADS-box from other plants. PsJOINTLESS was subcellularly targeted to the nucleus, supporting its role as a transcription factor. Expression levels of PsJOI...
Source: Gene - November 8, 2017 Category: Genetics & Stem Cells Authors: Qi X, Hu S, Zhou H, Liu X, Wang L, Zhao B, Huang X, Zhang S Tags: Gene Source Type: research

The role of TRPV4 in fibrosis.
Abstract The transient receptor potential vanilloid 4 (TRPV4) is a highly Ca(2+)-permeable non-selective cation channel in TRPV family. Accumulating evidence hints that TRPV4 play a significant role in a wide diversity of pathologic changes. Fibrosis is a kind of chronic disease which was characterized by the formation of excessive accumulation of extracellular matrix (ECM) components in tissues and organs. In recent years, a growing body of studies showed that TRPV4 acted as a crucial regulator in the progression of fibrosis including myocardial fibrosis, cystic fibrosis, pulmonary fibrosis, hepatic fibrosis and ...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Zhan L, Li J Tags: Gene Source Type: research

The unrecognized role of tumor suppressor genes in atrial fibrillation.
Abstract Epidemiogical evidence has shown that the incidence of atrial fibrillation in tumor patients is higher than non-tumor patients and general population. The potential risk factors predisposing tumor patients to atrial fibrillation include advanced age, comorbidities, direct anatomic local occupying effect of tumors in the heart or adjacent organs, paraneoplastic manifestations of some tumors, tumor-induced dys-regulation of metabolism, radio-, bio- and chemo-therapeutics, disturbance of autonomous nerve system because of physical pain and psychological sufferings, chronic inflammation typical of most tumors...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Mao L, Huang W, Zou P, Dang X, Zeng X Tags: Gene Source Type: research

CLOCK interacts with RANBP9 and is involved in alternative splicing in spermatogenesis.
Abstract The core circadian gene CLOCK plays an important role in regulating male reproduction. However, the underlying mechanism still remains unclear. In the present study, we executed yeast two-hybrid screening using cDNA fragment of CLOCK PAS A domain as bait, and identified RANBP9 as a novel protein interacting with CLOCK. The interaction between CLOCK and RANBP9 was further validated by in vivo and in vitro assays. Previous studies have confirmed that SF3B3 was a RANBP9 interacting protein. Subsequently, our study also found that CLOCK and SF3B3 can interact with each other by co-immunoprecipitation in mouse...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Yang J, Zhang Z, Zhang Y, Zheng X, Lu Y, Tao D, Liu Y, Ma Y Tags: Gene Source Type: research

Suppression of p53R2 gene expression with specific siRNA sensitizes HepG2 cells to doxorubicin.
Abstract INTRODUCTION: p53R2 is a p53-inducible protein that contributes to DNA repair by providing dNTPs in response to DNA damage. The roles of p53R2 in cancer cells and malignancies still remain controversial. Herein, we examined the effects of p53R2 silencing on HepG2 human hepatocellular carcinoma (HHC) cell line (wild-type p53) viability, apoptosis and cell cycle arrest in the presence and absence of doxorubicin. METHODS: Cell transfection was performed using a liposomal approach. Gene silencing was determined by quantitative real-time PCR and western blot analysis. To evaluate the cell growth rate afte...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Azimi A, Majidinia M, Shafiei-Irannejad V, Jahanban-Esfahlan R, Ahmadi Y, Karimian A, Mir SM, Karami H, Yousefi B Tags: Gene Source Type: research

Comparative transcriptomic characterization of a new mib mutant allele, mib(nn2002), in zebrafish.
Abstract mib(nn2002), identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mib(nn2002) mutants. By comparing the transcriptomic data of mib(nn2002) mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 ...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Lai KP, Li JW, Hsu CH, You MS, Chan TF, Tse WKF, Jiang YJ Tags: Gene Source Type: research

AJUBA increases the cisplatin resistance through hippo pathway in cervical cancer.
Abstract Though LIM-domain protein AJUBA was identified as a putative oncogene, the function and underlying mechanisms of AJUBA in cervical cancer remain largely unknown. Firstly, AJUBA expression was detected via real-time quantitative PCR in patients' samples. Furthermore, Hela and Siha cells were transfected with AJUBA-overexpressing plasmids, and then exposed to cisplatin, the apoptosis was measured by cytometry assay. In addition, the expression of YAP and TAZ was disclosed through western blot assay. Our results revealed that AJUBA expression was significantly higher in the cervical cancer patients resistant...
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Bi L, Ma F, Tian R, Zhou Y, Lan W, Song Q, Cheng X Tags: Gene Source Type: research

Interleukin-33 levels are elevated in chronic allograft dysfunction of kidney transplant recipients and promotes epithelial to mesenchymal transition of human kidney (HK-2) cells.
This study is aimed to investigate the potential role of interleukin (IL)-33 in transplanted kidney interstitial fibrosis and the associated mechanism. Serum IL-33 levels were detected using an enzyme-linked immunosorbent assay (ELISA) in healthy volunteers, stable kidney transplantation recipients (KTRs) (stable), KTRs with acute rejection (AR), and KTRs with chronic allograft dysfunction (CAD) (CAD). Immunohistochemical (IHC) staining, Western blotting, and quantitative real-time PCR (qRT-PCR) were used to detect the expression of IL-33 in human kidney tissues obtained from control and CAD patients. In addition, human ki...
Source: Gene - November 6, 2017 Category: Genetics & Stem Cells Authors: Xu Z, Zhao C, Wang Z, Tao J, Han Z, Zhang W, Tan R, Gu M Tags: Gene Source Type: research

Drug response prediction in high-risk multiple myeloma.
Abstract A Drug Response Prediction (DRP) score was developed based on gene expression profiling (GEP) from cell lines and tumor samples. Twenty percent of high-risk patients by GEP70 treated in Total Therapy 2 and 3A have a progression-free survival (PFS) of more than 10years. We used available GEP data from high-risk patients by GEP70 at diagnosis from Total Therapy 2 and 3A to predict the response by the DRP score of drugs used in the treatment of myeloma patients. The DRP score stratified patients further. High-risk myeloma with a predicted sensitivity to melphalan by the DRP score had a prolonged PFS, HR=2.4 ...
Source: Gene - November 6, 2017 Category: Genetics & Stem Cells Authors: Vangsted AJ, Helm-Petersen S, Cowland JB, Jensen PB, Gimsing P, Barlogie B, Knudsen S Tags: Gene Source Type: research

RNA-Seq-derived identification of differential transcription in the eggplant (Solanum melongena) following inoculation with bacterial wilt.
In conclusion, our results provide useful insights into the potential mechanism of BW and are an important basis for further analysis. PMID: 29104166 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Chen N, Yu B, Dong R, Lei J, Chen C, Cao B Tags: Gene Source Type: research

The tubby-like proteins kingdom in animals and plants.
Abstract Each gene of the tubby-like family is characterized by a signature of C-terminal tubby domain. The wide spread of this family in plants and animals implies they have an important function in various organisms. Even though the tubby-like genes are suggested to be putative transcription factors (TFs), how they execute the function as TFs is not yet clear. The biological functions of most animal tubby-like genes have been well studied, especially for vertebrate TUB, TULP1 and TULP3, but not with TULP2 and TULP4. Plants possess more tubby-like genes than animals, but their functions are still elusive except t...
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Wang M, Xu Z, Kong Y Tags: Gene Source Type: research

Evaluation of PCSK9 levels and its genetic polymorphisms in women with polycystic ovary syndrome.
The objective of this study was to evaluate the PCSK9 polymorphisms rs505151 (c.2009A>G), rs562556 (c.1420A>G) and rs11206510 (T>C) and plasma PCSK9 levels in PCOS. A group of women with PCOS (n=97), and a group of healthy women (control, n=99) were selected. Biochemical parameters were determined by using Vitros system and polymorphisms were assessed by TaqMan SNP Genotyping Assays. Plasma PCSK9 levels or PCSK9 polymorphisms were not associated with PCOS. The genotype rs11206510TT was associated with higher levels of PCSK9 in both groups. The population investigated (PCOS+control groups) with the rs505151AA genot...
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Xavier LB, Sóter MO, Sales MF, Oliveira DK, Reis HJ, Candido AL, Reis FM, Silva IO, Gomes KB, Ferreira CN Tags: Gene Source Type: research

The rs2292239 polymorphism in ERBB3 gene is associated with risk for type 1 diabetes mellitus in a Brazilian population.
Abstract The Erb-b2 receptor tyrosine kinase 3 (ERBB3) belongs to a family of epidermal growth factor receptors of protein tyrosine kinases, and regulates cell survival, differentiation and proliferation in several cell types. Previous studies have suggested that ERBB3 contributes to T1DM pathogenesis by modulating antigen presenting cell function, autoimmunity and cytokine-induced beta-cell apoptosis. Accordingly, some genome-wide association studies identified ERBB3 gene as a susceptibility locus for T1DM, with the strongest association signal being observed for the rs2292239 single nucleotide polymorphism (SNP)...
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Lemos NE, Dieter C, Dorfman LE, Assmann TS, Duarte GCK, Canani LH, Bauer AC, Crispim D Tags: Gene Source Type: research

Roles of DEK in the endometrium of mice in early pregnancy.
In this study, we investigated the expression of DEK in mouse uterus by immunohistochemistry (IHC), Western blotting. We explored its function during decidualization of uterine stromal cells by inhibiting the expression of DEK. In further study of mechanism, the cell proliferation, apoptosis and DNA damage were detected after inhibiting DEK during decidualization of stromal cells. The results suggest that DEK participates in decidualization of stromal cells through mediating cell proliferation, apoptosis and DNA repair. PMID: 29109007 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Yang Y, Wu M, Geng Y, Liu X, Yang Y, Chen X, Ding Y, He J, Wang Y, Xie L Tags: Gene Source Type: research

The pathogenic gene screening in a Chinese familial dilated cardiomyopathy pedigree from Hubei.
In this study, we report a Chinese family with two members affected by TTN. Blood samples were collected from all family members. Genomic DNA was isolated from blood, and all coding exons and adjacent intronic sequences of the TTN gene were examined for mutation analysis using polymerase chain reaction (PCR)-based sequencing. The proband (III3) and his sister (III2) carry a TTN c.100126A>G (p.Thr33376Ala) missense mutation. The proband currently exhibits decreased cardiac function accompanied by malignant arrhythmia, and his sister has no obvious clinical symptoms and no abnormal ultrasound findings. The study found tha...
Source: Gene - November 3, 2017 Category: Genetics & Stem Cells Authors: Lyu Y, Chen J, Xu H Tags: Gene Source Type: research

Novel Polymerase Spiral Reaction (PSR) for rapid visual detection of Bovine Herpesvirus 1 genomic DNA from aborted bovine fetus and semen.
Abstract Bovine herpesvirus-1 (BHV-1) is a major viral pathogen affecting bovines leading to various clinical manifestations and causes significant economic impediment in modern livestock production system. Rapid, accurate and sensitive detection of BHV-1 infection at frozen semen stations or at dairy herds remains a priority for control of BHV-1 spread to susceptible population. Polymerase Spiral Reaction (PSR), a novel addition in the gamut of isothermal techniques, has been successfully implemented in initial optimization for detection of BHV-1 genomic DNA and further validated in clinical samples. The develope...
Source: Gene - November 2, 2017 Category: Genetics & Stem Cells Authors: Malla JA, Chakravarti S, Gupta V, Chander V, Sharma GK, Qureshi S, Mishra A, Gupta VK, Nandi S Tags: Gene Source Type: research

Eukaryotic and prokaryotic promoter databases as valuable tools in exploring the regulation of gene transcription: A comprehensive overview.
Abstract The complete exploration of the regulation of gene expression remains one of the top-priority goals for researchers. As the regulation is mainly controlled at the level of transcription by promoters, study on promoters and findings are of great importance. This review summarizes forty selected databases that centralize experimental and theoretical knowledge regarding the organization of promoters, interacting transcription factors (TFs) and microRNAs (miRNAs) in many eukaryotic and prokaryotic species. The presented databases offer researchers valuable support in elucidating the regulation of gene transcr...
Source: Gene - November 2, 2017 Category: Genetics & Stem Cells Authors: Majewska M, Wysokińska H, Kuźma Ł, Szymczyk P Tags: Gene Source Type: research

Protein secondary structure prediction based on the fuzzy support vector machine with the hyperplane optimization.
Abstract The prediction of the protein secondary structure is a crucial point in bioinformatics and related fields. In the last years, machine learning methods have become a valuable tool, achieving satisfactory results. However, the prediction accuracy needs to be further ameliorated. This paper proposes a new method based on an improved fuzzy support vector machine (FSVM) for the prediction of the secondary structure of proteins. Unlike traditional methods to set the membership function, it firstly constructs an approximate optimal separating hyperplane by iterating the class centers in the feature space. Then s...
Source: Gene - November 2, 2017 Category: Genetics & Stem Cells Authors: Xie S, Li Z, Hu H Tags: Gene Source Type: research