Identification of key genes, MicroRNAs and potentially regulated pathways in alcoholic hepatitis by integrative analysis.
Abstract Alcoholic hepatitis (AH) is a severe form of alcoholic liver disease associated with high mortality. Current pharmacological treatment options are not fully effective, and novel target therapies are urgently needed. Until now, key genes, miRNAs and potential signaling pathways in AH remain unclear. Here, we integrated mRNA and miRNA expression profiles to reveal 1411 differentially expressed genes (DEG) and 69 differentially expressed miRNAs (DEM) in AH. And then 51 overlapping genes were identified by compared with miRNA target genes and DEGs, which named as consistent expression genes (CEGs). Pathway an...
Source: Gene - August 9, 2019 Category: Genetics & Stem Cells Authors: Yao J, Cheng Y, Zhang D, Fan J, Zhao Z, Li Y, Jiang Y, Guo Y Tags: Gene Source Type: research

Exosomes derived from liver cancer cells reprogram biological behaviors of LO2 cells by transferring Linc-ROR.
CONCLUSIONS: Our experiments indicated the HepG2 cells could transfer its Linc-ROR to the LO2 cells via exosomes, then influenced the recipient cells. PMID: 31400406 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 7, 2019 Category: Genetics & Stem Cells Authors: He X, Yu J, Xiong L, Liu Y, Fan L, Li Y, Chen B, Chen J, Xu X Tags: Gene Source Type: research

The dual function gene RAD23 contributes to Cryptococcus neoformans virulence independently of its role in nucleotide excision DNA repair.
Abstract Genes involved in the repair of DNA damage are emerging as playing important roles during the disease processes caused by pathogenic fungi. However, there are potentially hundreds of genes involved in DNA repair in a fungus and some of those genes can play additional roles within the cell. One such gene is RAD23, required for virulence of the human pathogenic fungus Cryptococcus neoformans, that encodes a protein involved in the nucleotide excision repair (NER) pathway. However, Rad23 is a dual function protein, with a role in either repair of damaged DNA or protein turn over by directing proteins to the ...
Source: Gene - August 7, 2019 Category: Genetics & Stem Cells Authors: Verma S, Shakya VPS, Idnurm A Tags: Gene Source Type: research

Genetic mutation analysis of the COQ2 gene in Italian patients with multiple system atrophy.
Abstract COQ2 encodes para-hydroxybenzoate-polyprenyl transferase and, recently, mutations in this gene have been associated with the increase of the risk of multiple system atrophy (MSA) in Japanese cases. Subsequently, studies in Asian patients confirmed the role of COQ2 in the development of MSA, while other analysis failed to replicate these results in Caucasian population. We performed genetics screening of COQ2 in 100 MSA Italian patients. We did not find any pathogenic mutations; our results suggest that COQ2 is not a genetic risk factor for MSA in Italian population. PMID: 31398377 [PubMed - as suppli...
Source: Gene - August 6, 2019 Category: Genetics & Stem Cells Authors: Procopio R, Gagliardi M, Brighina L, Nicoletti G, Morelli M, Ferrarese C, Annesi G, Quattrone A Tags: Gene Source Type: research

Integrative data mining and meta-analysis to investigate the prognostic role of microRNA-200 family in various human malignant neoplasms: A consideration on heterogeneity.
We presented the expressions of each member in miR-200 family in 15 types of cancer by boxplot, and analyzed the correlation among the members of miR-200 family by Spearman method. Different subgroup analyses were then performed based on the correlation among the members of miR-200 family, and the publication bias was assessed using the funnel plot of the Egger bias indicator test. RESULTS: Of 36 articles, including 15 tumor types and 4644 patients were included to perform meta-analysis. It was found that miR-200 family members can be used as independent protective factors in patients with various tumors but the miR-2...
Source: Gene - August 5, 2019 Category: Genetics & Stem Cells Authors: Yuan L, Bing Z, Yan P, Li R, Wang C, Sun X, Yang J, Shi X, Zhang Y, Yang K Tags: Gene Source Type: research

The effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells lines.
lkanlı M, Vatansever S Abstract Opioids are widely used in the treatment of cancer related pain. They mainly exert their effects on opioid receptors. The most common opioid in the treatment of pain is morphine. Previous studies show that they may have effects on cancer cell behavior. These may include apoptosis, angiogenesis, invasion, inflammation and immune reactions. Tramadol, also an opioid is widely used in the treatment of cancer pain and is not well studied in cancer behavior. We aimed to investigate the effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells. We used Colo32...
Source: Gene - August 4, 2019 Category: Genetics & Stem Cells Authors: Özgürbüz U, Gencür S, Kurtun FÖ, Özkalkanlı M, Vatansever S Tags: Gene Source Type: research

Sequencing of anthocyanin synthesis-related enzyme genes and screening of reference genes in leaves of four dominant subtropical forest tree species.
This study has important theoretical and practical significance for future research into the expression of anthocyanin synthesis-related enzyme genes in the dominant tree species in subtropical forests and reveals that anthocyanin has a photoprotective effect for young leaves in high-light environments. PMID: 31390541 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 4, 2019 Category: Genetics & Stem Cells Authors: Yu Z, Zhang P, Lin W, Zheng X, Cai M, Peng C Tags: Gene Source Type: research

Transcriptome profiling in Eid1-KO mice brain shows that Eid1 links cell proliferation in the brain.
This study identified 2531 genes differentially expressed in Eid1-KO mice compared with the wild-type, then qRT-PCR verification demonstrated that the transcriptomic data are reliable. By protein-protein interaction cluster analysis, 'regulation of cell proliferation' were unexpectedly discovered as important Eid1 functions. We then isolated neural progenitor cells (NPCs) and showed that the number of neurospheres and the proliferation rate of Eid1-KO NPCs were obviously lower than that in the control group, furthermore, CCK-8 and immunofluorescence assay clearly demonstrated that the Eid1-KO NPCs showed significantly less...
Source: Gene - August 2, 2019 Category: Genetics & Stem Cells Authors: Fu X, Luo L, Yi R, Ding B, Wang C, Zhang W, Wang X, Yang Y, Chen C, Fei X, Hu D, Xu R Tags: Gene Source Type: research

Transcripts of the nebulin gene from Ciona heart and their implications for the evolution of nebulin family genes.
In this study, we investigated the structure and cardiac expression of the nebulin gene in Ciona intestinalis, a urochordate whose phylogeny lies between cephalochordates and vertebrates. As a result of the gene structure analysis, we found that the Ciona nebulin gene predicted to be 62 kb and consists of 143 exons. The nebulin was expected to consist of a unique N-terminal region, followed by 155 nebulin repeats, another unique region, a Ser-rich region and a C-terminal SH3 domain. Whole-mount in situ hybridization experiments showed that the Ciona nebulin gene was expressed in a variety of muscles, including hearts. Ho...
Source: Gene - August 2, 2019 Category: Genetics & Stem Cells Authors: Nishikawa A, Hanashima A, Nakayama S, Ogasawara M, Kimura S Tags: Gene Source Type: research

Corrigendum to "Breast cancer invasion and progression by MMP-9 through Ets-1 transcription factor" [Gene 711 (2019) 143952].
Corrigendum to "Breast cancer invasion and progression by MMP-9 through Ets-1 transcription factor" [Gene 711 (2019) 143952]. Gene. 2019 Aug 01;:144013 Authors: Nazir SU, Kumar R, Singh A, Khan A, Tanwar P, Tripathi R, Mehrotra R, Hussain S PMID: 31377019 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Nazir SU, Kumar R, Singh A, Khan A, Tanwar P, Tripathi R, Mehrotra R, Hussain S Tags: Gene Source Type: research

Transfection of microRNA-143 mimic could inhibit migration of HN-5 cells through down-regulating of metastatic genes.
Abstract Oral squamous cell cancer (OSCC) is one of the causes of death worldwide. The purpose of this project was to define the restoring of microRNA-143 in HN-5 cells and discover molecular apparatuses responsible for the anticancer processes. Firstly, expression levels of miR-143, K-Ras, MMP9 and C-Myc were evaluated in OSCC tissues. Then, microRNA-143 was transfected into HN-5 cells. The cytotoxic effects of microRNA-143 on HN-5 cells were evaluated. To estimate the effects of microRNA-143 on cell migration, wound healing assay was done. The expression levels of microRNA-143, K-Ras, MMP9, C-Myc, ADAMTS and CXC...
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Mesgarzadeh AH, Aali M, Farhadi F, Noorolyai S, Baghbani E, Mohammadnejad F, Baradaran B Tags: Gene Source Type: research

Circ-IGF1R has pro-proliferative and anti-apoptotic effects in HCC by activating the PI3K/AKT pathway.
In this study, we identified a novel circRNA, circ-IGF1R, in HCC tumour tissues and cell lines. Circ-IGF1R levels were found to be significantly upregulated in HCC tissues compared with levels in paired peritumoural tissues. The high expression levels of circ-IGF1R in HCC were associated with tumour size. Moreover, knocking down circ-IGF1R with siRNA significantly attenuated cell proliferation and induced cell apoptosis and cell cycle arrest in vitro. Further investigation revealed that PI3K/AKT signalling pathway activation was involved in the oncogenic functions of circ-IGF1R in HCC. Our study suggests that circ-IGF1R ma...
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Fu HW, Lin X, Zhu YX, Lan X, Kuang Y, Wang YZ, Ke ZG, Yuan T, Chen P Tags: Gene Source Type: research

Mitogenomes of Spondias.
Abstract Mitogenomes in plants are well-known as exhibiting high diversity in genome size architecture and repetitive DNA sequences. In this research study, we report on the complete mitochondrial genomes of S. tuberosa and S. mombin using Illumina paired-end and mate-pair end reads. These genomes were obtained by a combination of methods of de novo assembly and contig extension. The mitogenomes of S. tuberosa and S. mombin showed 779,106 bp and 674,156 bp in length, with a total of 49 and 47 genes, respectively. Genome comparisons showed many rearrangements that were mediated by repetitive DNA, and also high ...
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Martins G, Balbino E, Marques A, Almeida C Tags: Gene Source Type: research

Mitochondrial MTHFD isozymes display distinct expression, regulation, and association with cancer.
This study supports that MTHFD2L is unlikely to have an important function in increased proliferation or cancer. Furthermore, therapeutic strategies aiming to block the mitochondrial folate pathway in cancer should focus on MTHFD2, with MTHFD2L being unlikely to be involved in the development of chemoresistance to targeting of its mitochondrial isozyme. PMID: 31377316 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Nilsson R, Nicolaidou V, Koufaris C Tags: Gene Source Type: research

Histone chaperone CHAF1A impacts the outcome of fluoropyrimidines-based adjuvant therapy in gastric cancer by regulating the expression of thymidylate synthetase.
Abstract BACKGROUND: Outcome in adjuvant chemotherapy of gastric cancer (GC) has considerable stage-independent variability, which underscores the need for prognostic or predictive molecular markers. CHAF1A promotes tumor growth while its impact on chemotherapy outcome remains unknown. METHODS: CHAF1A protein expression was measured in independent discovery and validation sets that included 86 and 325 patients respectively who received fluoropyrimidines-based adjuvant chemotherapy after radical gastrectomy. The chemosensitizing effect of CHAF1A knockdown was investigated in vitro. Bioinformatics analysis base...
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Wang D, Li X, Shen B, Chen X, Shu Y Tags: Gene Source Type: research

Genomic and biological features of Plasmodium falciparum resistance against antimalarial endoperoxide N-89.
In this study we seek to understand the resistant mechanism against N-89 by establishing a highly N-89-resistant clone, named NRC10H, of the Plasmodium falciparum FCR-3 strain. We describe gene mutations in the parent FCR-3 strain and the NRC10H clone using whole-genome sequencing and subsequently by expression profiling using quantitative real-time PCR. Seven genes related to drug resistance, proteolysis, glycophosphatidylinositol anchor biosynthesis, and phosphatidylethanolamine biosynthesis exhibited a single amino acid substitution in the NRC10H clone. Among these seven genes, the multidrug resistance protein 2 (mdr2) ...
Source: Gene - August 1, 2019 Category: Genetics & Stem Cells Authors: Morita M, Hayashi K, Sato A, Hiramoto A, Kaneko O, Isogawa R, Kurosaki Y, Miyoshi SI, Chang KS, Wataya Y, Kim HS Tags: Gene Source Type: research

The long noncoding RNA HOTAIR serves as a microRNA-34a-5p sponge to reduce nucleus pulposus cell apoptosis via a NOTCH1-mediated mechanism.
This study sought to assess to what extent HOTAIR, a long non-coding (Lnc) RNA is expressed in IDD and regulates the apoptotic death of nucleus pulposus (NP) cells. We therefore used real-time qPCR to measure HOTAIR and microRNA(miR)-34a-5p in degenerative NP cells, and then validated their functional relevance via overexpressing them in these NP cells. We further verified the targets of these RNA constructs in 293 T cells through the use of a dual luciferase reporter assay. We further measured NP cell apoptosis via flow cytometry and Notch1 expression via western blotting. Our results indicated that IDD was linked with ...
Source: Gene - July 31, 2019 Category: Genetics & Stem Cells Authors: Shao T, Hu Y, Tang W, Shen H, Yu Z, Gu J Tags: Gene Source Type: research

Structure, regulation, and (patho-)physiological functions of the stress-induced protein kinase CK1 delta (CSNK1D).
f J Abstract Members of the highly conserved pleiotropic CK1 family of serine/threonine-specific kinases are tightly regulated in the cell and play crucial regulatory roles in multiple cellular processes from protozoa to human. Since their dysregulation as well as mutations within their coding regions contribute to the development of various different pathologies, including cancer and neurodegenerative diseases, they have become interesting new drug targets within the last decade. However, to develop optimized CK1 isoform-specific therapeutics in personalized therapy concepts, a detailed knowledge of the regulatio...
Source: Gene - July 31, 2019 Category: Genetics & Stem Cells Authors: Xu P, Ianes C, Gärtner F, Liu C, Burster T, Bakulev V, Rachidi N, Knippschild U, Bischof J Tags: Gene Source Type: research

GNAS gene is an important regulator of insulin secretory capacity in pancreatic β-cells.
CONCLUSION: LoFtool is a powerful tool to identify genes associated with pancreatic islets dysfunction. GNAS is a crucial gene for the β-cell insulin secretory capacity. PMID: 31374326 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 30, 2019 Category: Genetics & Stem Cells Authors: Taneera J, Dhaiban S, Mohammed AK, Mukhopadhyay D, Aljaibeji H, Sulaiman N, Fadista J, Salehi A Tags: Gene Source Type: research

A novel mutation of the ITGB2 gene in a Chinese Zhuang minority patient with leukocyte adhesion deficiency type 1 and glucose-6-phosphate dehydrogenase deficiency.
CONCLUSIONS: The patient was diagnosed with G6PDD and moderate LAD-1. The combination of LAD-1 and G6PDD in this case may have been due to the high incidence of genetic disease in this minority ethnic population. Analyzing existing LAD-1 and G6PDD cases from different populations can facilitate disease diagnosis and treatment. Particularly, reporting pathogenic mutations of LAD-1 and G6PDD will be crucial for genetic testing and prenatal diagnosis in an effort to decrease the incidence of these diseases. PMID: 31374327 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 30, 2019 Category: Genetics & Stem Cells Authors: Zhang Y, Yang X, He X, Liu H, Guo P, Liu X, Xiao Y, Feng X, Wang Y, Li L Tags: Gene Source Type: research

Polymorphisms of four candidate genes and their correlations with growth traits in blue fox (Alopex lagopus).
Abstract To improve the accuracy and genetic progress of blue fox breeding, four genes that have a relatively large influence on growth traits were selected as candidate genes. χ2 goodness-of-fit test and Linkage disequilibrium (LD) analysis are applied to mutation site. In addition, a single factor variance analysis showed that the MC4R (g.267C > T, g.423C > T, and g.731C > A) and MC3R (g.677C > T) genotypes had significant impacts on body weight, chest circumference, abdominal perimeter and body mass index (BMI) (P  A had significant effects on female fecundity, whereas the di...
Source: Gene - July 27, 2019 Category: Genetics & Stem Cells Authors: Yu DY, Wu RZ, Zhao Y, Nie ZH, Wei L, Wang TY, Liu ZP Tags: Gene Source Type: research

Gene regulation for the extreme resistance to ionizing radiation of Deinococcus radiodurans.
Abstract Deinococcus radiodurans is a model microorganism used for studies on DNA repair and antioxidation due to its extraordinary tolerance to ionizing radiation and other DNA-damaging agents. Various transcriptome analyses have revealed that hundreds of genes are induced and that many other genes are repressed during recovery of D. radiodurans following irradiation, suggesting that gene regulation is of great importance for the extreme resistance of this microorganism to ionizing radiation. In this article, we focus on some reported strategies that are employed by D. radiodurans to regulate the genes implicated...
Source: Gene - July 27, 2019 Category: Genetics & Stem Cells Authors: Wang W, Ma Y, He J, Qi H, Xiao F, He S Tags: Gene Source Type: research

Association between F+1 polymorphism in a disintegrin and metalloprotease 33 (ADAM33) gene and chronic obstructive pulmonary disease susceptibility: An evidence-based meta-analysis.
CONCLUSION: The ADAM33 F+1 mutant gene A may increase the risk of COPD among the Asian population, while it may not associate with the European population. PMID: 31357020 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Feng HH, Mao L, Pan K, Zhang L, Rui DS Tags: Gene Source Type: research

Integrative analysis of DNA methylation and gene expression profiles identifies MIR4435-2HG as an oncogenic lncRNA for glioma progression.
In this study, we firstly analyzed the RNA-seq data systematically across grades II, IV, and IV of glioma samples. We identified 60 lncRNAs that are significantly differentially expressed over disease progression (DElncRNA), including well-known PVT1, HOTAIR, H19 and rarely studied CARD8-AS, MIR4435-2HG. Secondly, by integrating HM450K methylation microarray data, we demonstrated that some of the lncRNAs are epigenetically regulated by methylation. Thirdly, we developed a DESeq2-GSEA-ceRNA-survival analysis strategy to investigate their functions. Particularly, MIR4435-2HG is highly expressed in high-grade glioma and may h...
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Li Z, Tan H, Zhao W, Xu Y, Zhang Z, Wang M, Zhou X Tags: Gene Source Type: research

Apolipoprotein E genetic polymorphism influence the susceptibility to nephropathy in type 2 diabetes patients.
CONCLUSION: The results of this case-control study provide evidence that the ε2 and ε3 alleles of APOE modify lipid profile, and constitute independent risk factors of DN in type 2 diabetes. The molecular mechanisms underlying this risk is discussed. PMID: 31357022 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Atageldiyeva KK, Nemr R, Echtay A, Racoubian E, Sarray S, Almawi WY Tags: Gene Source Type: research

Genetic variants in 17q12-21 locus and childhood asthma in Brazil: Interaction with Varicella zoster virus seropositivity.
This study aimed to investigate the role of variants in the 17q12-21 locus on asthma in a sample of Brazilian children. METHODS: This was a cross-sectional study conducted on a cohort of 1247 children. These analyses used 50 Single Nucleotide Variants (SNVs) in the 17q12-21 locus were genotyped as part of a genome wide association study (GWAS). RESULTS: Four SNVs (rs4065275, rs12603332, rs73985228 and rs77777702) were associated with childhood asthma. The rs73985228 exhibited the strongest association across the different genetic models (OR, 95%CI 2.8, 1.44-3.21, p 
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: de Oliveira Costa GN, Figueiredo CA, Conceição JS, Strina A, Genser B, da Silva TM, Alcantara-Neves NM, Fiaccone RL, Rodrigues LC, Barreto ML Tags: Gene Source Type: research

A novel mutation (-195C > A) in the promoter region of CFTR gene is associated with Chinese Congenital Bilateral Absence of Vas Deferens (CBAVD).
This study aim to explore the promoter region of CFTR gene in CBAVD patients and study the mutations by functional analysis, and to discuss the significance of mutation testing in this area. We performed screening analysis on 65 CBAVD patients and 50 controls to detect mutations in the CFTR gene, and studied the functions of promoter mutations using reporter gene constructs, transient transfection techniques and subsequent assessment of transcriptional activity and expression levels. Mutations c.-195C>A and c.-34C>T in the promoter region of the CFTR gene were detected in 4 of our Chinese CBAVD patients, one of which...
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Feng J, Wu X, Zhang Y, Yang X, Ma G, Chen S, Luo S, Zhang Y Tags: Gene Source Type: research

TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF- κB pathway in non-small-cell lung carcinoma cells.
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. Gene. 2019 Jul 26;:144015 Authors: Xu L, Wu Q, Zhou X, Wu Q, Fang M Abstract Tripartite Motif Containing 13 (TRIM13), a member of TRIM proteins, is deleted in multiple tumor types, especially in B-cell chronic lymphocytic leukemia and multiple myeloma. The present study explored the expression and potential role of TRIM13 in non-small-cell lung carcinoma (NSCLC). We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in compariso...
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Xu L, Wu Q, Zhou X, Wu Q, Fang M Tags: Gene Source Type: research

SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling.
Abstract SRY-related high-mobility-group box 9 (SOX9) is a member of the SOX family of transcription factors. Accumulating evidence has shown that SOX9 plays a significant role in various malignancies. However, the role of SOX9 in nasopharyngeal carcinoma (NPC) remains unknown. In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. Conversely, knock down of SOX9 inhibited NPC proliferation, colony formation, migration and invasion. Mechanistically, SOX9 bound directly to the promoter regio...
Source: Gene - July 26, 2019 Category: Genetics & Stem Cells Authors: Xiao B, Zhang W, Kuang Z, Lu J, Li W, Deng C, He Y, Lei T, Hao W, Sun Z, Li L Tags: Gene Source Type: research

Knockdown of SSATX, an alternative splicing variant of the SAT1 gene, promotes melanoma progression.
Abstract Alternative splicing can generate multiple protein messages from a single gene and has emerged as an important mechanism to regulate cancer pathways. The human SAT1 gene produces two transcript variants: one translates spermidine/spermine N-1 acetyltransferase (SSAT1), the rate-limiting enzyme in the catabolism of polyamines, and the other generates SSATX, which has largely unknown biological functions. Here, we used experimental data and analyses of several melanoma transcriptome datasets to reveal that SSATX is weakly expressed in melanoma cells. SSATX knockdown promoted the proliferation, migration, an...
Source: Gene - July 25, 2019 Category: Genetics & Stem Cells Authors: Yang Q, Deng Y, Xu Y, Ding N, Wang C, Zhao X, Lou X, Li Y, Zhao H, Fang X Tags: Gene Source Type: research

Role of calreticulin in biotic and abiotic stress signalling and tolerance mechanisms in plants.
Abstract Calreticulin (CRT) is calcium binding protein of endoplasmic reticulum (ER) which performs plethora of functions besides it's role as molecular chaperone. Among the three different isoforms of this protein, CRT3 is most closely related to primitive CRT gene of higher plants. Based on their distinct structural and functional organisation, the plant CRTs have been known to contain three different domains: N, P and the C domain. The domain organisation and various biochemical characterstics of plant and animal CRTs are common with the exception of some differences. In plant calreticulin, the important N-glyc...
Source: Gene - July 24, 2019 Category: Genetics & Stem Cells Authors: Joshi R, Paul M, Kumar A, Pandey D Tags: Gene Source Type: research

Global proteomic profiling of the uniquely human CHRFAM7A gene in transgenic mouse brain.
Abstract The uniquely human α7-nAChR gene (CHRFAM7A) is evolved from the fusion of two partially duplicated genes, FAM7 and α7-nAChR gene (CHRNA7), and is inserted on same chromosome 15, 5' end of the CHRNA7 gene. Transcription of CHRFAM7A gene produces a 1256-bp open reading frame encoding dup-α7-nAChR, where a 27-aminoacid residues from FAM7 replaced the 146-aminoacid residues of the N-terminal extracellular ligand binding domain of α7-nAChR. In vitro, dup-α7-nAChR has been shown to form hetero-pentamer with α7-nAChR and dominant-negatively regulates the channel functions of &...
Source: Gene - July 23, 2019 Category: Genetics & Stem Cells Authors: Jiang Y, Yuan H, Huang L, Hou X, Zhou R, Dang X Tags: Gene Source Type: research

Akt1 and Jak1 siRNA based silencing effects on the proliferation and apoptosis in head and neck squamous cell carcinoma.
In conclusion, the combination of siRNA-mediated gene-silencing strategy can be considered as a valuable and safe approach for sensitizing cancer cells to chemotherapeutic agents thus proposed further studies regarding this issue to approve some siRNA based therapeutics for using in clinic. PMID: 31348981 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 23, 2019 Category: Genetics & Stem Cells Authors: Saatloo MV, Aghbali AA, Koohsoltani M, Khosroushahi AY Tags: Gene Source Type: research

Up-regulation of microRNA-203 inhibits myocardial fibrosis and oxidative stress in mice with diabetic cardiomyopathy through the inhibition of PI3K/Akt signaling pathway via PIK3CA.
Abstract Diabetic cardiomyopathy (DCM) refers to the myocardial dysfunction in the absence of coronary artery disease and hypertension. Recently, the role of microRNAs (miRs) in gene expression regulation has attracted much more attention. Studies have shown that the PI3K/Akt signaling pathway is involved in the growth, metabolism and apoptosis of myocardial cells. Therefore, this study aimed to explore the regulatory role of miR-203 in myocardial fibrosis in mice with DCM via involvement of the PI3K/Akt signaling pathway. Firstly, mouse model of diabetes mellitus (DM) was established and injected with agomir, ant...
Source: Gene - July 20, 2019 Category: Genetics & Stem Cells Authors: Yang X, Li X, Lin Q, Xu Q Tags: Gene Source Type: research

Long non-coding RNA SNHG1 functions as a competitive endogenous RNA to regulate PDCD4 expression by sponging miR-195-5p in hepatocellular carcinoma.
In this study, SNHG1 expression in hepatocellular carcinoma cells was detected by qRT-PCR. Proliferative and migratory potentials of hepatocellular carcinoma cells were determined by CCK-8 and Transwell assay, respectively. Then, the nude mice model of xenograft was employed to verify the effect of SNHG1 on tumor formation in vivo. We identified the potential target of SNHG1 through bioinformatics and dual-luciferase reporter gene. Furthermore, Western blot and RIP assay was used for clarifying their interaction and functions in regulating the development of hepatocellular carcinoma. Our results indicated a high expression...
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: Huang D, Wei Y, Zhu J, Wang F Tags: Gene Source Type: research

Overexpression of circ_0021093 circular RNA forecasts an unfavorable prognosis and facilitates cell progression by targeting the miR-766-3p/MTA3 pathway in hepatocellular carcinoma.
In conclusion, the circ_0021093/miR-766-3p/MTA3 regulatory axis may be an effective therapeutic target for HCC. PMID: 31330234 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: Liu L, Qi X, Gui Y, Huo H, Yang X, Yang L Tags: Gene Source Type: research

Sequencing of NOTCH1 gene in an Italian population with bicuspid aortic valve: Preliminary results from the GISSI OUTLIERS VAR study.
CONCLUSIONS: Completing the genotyping of 62 BAV patients we found 11 new variants in the NOTCH1 gene never yet reported. These findings confirm that the identification of new, clinically remarkable biomarkers for BAV requires a deeper genetic understanding of the NOTCH1 gene variants, which could be targeted by future diagnostic and therapeutic strategies. PMID: 31330235 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: Pileggi S, De Chiara B, Magnoli M, Franzosi MG, Merlanti B, Bianchini F, Moreo A, Romeo G, Russo CF, Rizzo S, Basso C, Martinelli L, Maseri A, VAR Study Group Tags: Gene Source Type: research

Functional diversification of tomato HsfA1 factors is based on DNA binding domain properties.
Abstract In all eukaryotes, the response to heat stress (HS) is dependent on the activity of HS transcription factors (Hsfs). Plants contain a large number of Hsfs, however, only members of the HsfA1 subfamily are considered as master regulators of stress response and thermotolerance. In Solanum lycopersicum, among the four HsfA1 members, only HsfA1a has been proposed to possess a master regulator function. We performed a comparative analysis of HsfA1a, HsfA1b, HsfA1c and HsfA1e at different levels of regulation and function. HsfA1a is constitutively expressed under control and stress conditions, while the other m...
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: El-Shershaby A, Ullrich S, Simm S, Scharf KD, Schleiff E, Fragkostefanakis S Tags: Gene Source Type: research

Genomic analysis of intrinsically disordered proteins in cereals: From mining to meaning.
Abstract Intrinsically disordered proteins (IDPs) are highly abundant in eukaryotic proteomes and involved in key biological and cellular processes. Although some resources of disordered protein predictions are available from animal and plant proteomes, those related to cereals are largely unknown. Here, we present an overview of IDPomes from Oryza sativa, Zea mays, Sorghum bicolor and Brachypodium distachyon. The work includes a comparative analysis with the model plant Arabidopsis thaliana. The data show that the intrinsic disorder content increases with the proteome size. Gene Ontology analysis reveals that IDP...
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: Choura M, Ebel C, Hanin M Tags: Gene Source Type: research

Diagnostic significance assessment of the circulating cell-free DNA in ovarian cancer: An updated meta-analysis.
CONCLUSIONS: Our meta-analysis found the qualitative detection (methylation); TNM stage I-IV, publication year 2011-2018 were related to more effective diagnostic accuracy for OC. However, serum-based cell-free DNA detection should be cautiously interpreted due to unclear factors. Hence, further large-scale longitudinal studies are required to validate the diagnostic potential of cell-free DNA. The present study provides to accrue knowledge of cell-free DNA levels for future researches. PMID: 31330238 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 19, 2019 Category: Genetics & Stem Cells Authors: Li B, Pu K, Ge L, Wu X Tags: Gene Source Type: research

MYC-driven regulation of long non-coding RNA profiles in breast cancer cells.
CONCLUSION: We observed that c-MYC regulates lncRNAs that have important roles on proliferation, cell cycle and etc. Further studies will give us a light to identify molecular mechanisms related to MYC-lncRNA regulatory pathways in breast cancer. PMID: 31326549 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 18, 2019 Category: Genetics & Stem Cells Authors: Tokgun PE, Tokgun O, Kurt S, Tomatır AG, Akca H Tags: Gene Source Type: research

A newly identified missense mutation in CLCA2 is associated with autosomal dominant cardiac conduction block.
CONCLUSION: Our findings indicate that a novel heterozygous missense mutation c.G1725T of the CLCA2 gene may be associated with heart block disease and the mutation in this gene may lead to sinus node lesions and conduction blocking. PMID: 31326550 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 18, 2019 Category: Genetics & Stem Cells Authors: Mao Z, Wang Y, Peng H, He F, Zhu L, Huang H, Huang X, Lu X, Tan X Tags: Gene Source Type: research

Further studies of ion channels in the electroreceptor of the skate through deep sequencing, cloning and cross species comparisons.
Abstract Our comparative studies seek to understand the structure and function of ion channels in cartilaginous fish that can detect very low voltage gradients in seawater. The principal channels of the electroreceptor include a calcium activated K channel whose α subunit is Kcnma1, and a voltage-dependent calcium channel, Cacna1d. It has also been suggested based on physiological and pharmacological evidence that a voltage-gated K channel is present in the basal membranes of the receptor cells which modulates synaptic transmitter release. Large conductance calcium-activated K channels (BK) are comprised of ...
Source: Gene - July 18, 2019 Category: Genetics & Stem Cells Authors: Clusin WT, Wu TH, Shi LF, Kao PN Tags: Gene Source Type: research

The study of genes and signal transduction pathways involved in mustard lung injury: A gene therapy approach.
Abstract Sulfur mustard (SM) is a destructive and harmful chemical agent for the eyes, skin and lungs that causes short-term and long-term lesions and was widely used in Iraq war against Iran (1980-1988). SM causes DNA damages, oxidative stress, and Inflammation. Considering the similarities between SM and COPD (Chronic Obstructive Pulmonary Disease) pathogens and limited available treatments, a novel therapeutic approach is not developed. Gene therapy is a novel therapeutic approach that uses genetic engineering science in treatment of most diseases including chronic obstructive pulmonary disease. In this review,...
Source: Gene - July 16, 2019 Category: Genetics & Stem Cells Authors: Arabipour I, Amani J, Mirhosseini SA, Salimian J Tags: Gene Source Type: research

MicroRNA-544 attenuates diabetic renal injury via suppressing glomerulosclerosis and inflammation by targeting FASN.
In conclusion, our finding demonstrated that miR-544 attenuates diabetic renal injury via suppressing glomerulosclerosis and inflammation by targeting FASN, suggesting that miR-544 might have therapeutic potential for the treatment of DN. PMID: 31323309 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - July 16, 2019 Category: Genetics & Stem Cells Authors: Sun T, Liu Y, Liu L, Ma F Tags: Gene Source Type: research

Analysis of genetic diversity and population structure using SSR markers and validation of a Cleavage Amplified Polymorphic Sequences (CAPS) marker involving the sodium transporter OsHKT1;5 in saline tolerant rice (Oryza sativa L.) landraces.
Abstract Naturally evolved saline tolerant rice landraces found along the coastline of India are a valuable genomic resource to explore the complex, polygenic nature of salinity tolerance. In the present study, a set of 28 genome wide SSR markers, 11 salt responsive genic SSR markers and 8 Saltol QTL linked SSR markers were used to estimate genetic relatedness and population structure within a collection of 47 rice landraces (including a tolerant and 2 sensitive checks) originating from geographically divergent coastal regions of India. All three marker types identified substantial genetic variation among the land...
Source: Gene - July 12, 2019 Category: Genetics & Stem Cells Authors: Jayabalan S, Pulipati S, Ramasamy K, Jaganathan D, Venkatesan SD, Vijay G, Kumari K, Raju K, Neelakantan HG, Venkataraman G Tags: Gene Source Type: research

Evolution of S100A3 and PAD3, two important genes for mammalian hair.
Abstract Hair is one of the defining characteristics of mammals. The hair shaft has a two-layer structure comprising the cortex, which is the inner layer and is composed of cortical cells, and the cuticle, which is the outermost layer. S100 calcium-binding protein A3 (S100A3) is expressed at high levels in the human hair cuticle. Arginine 51 of S100A3 protein is citrullinated specifically by peptidyl arginine deiminase 3 (PAD3), and this citrullination is related to maturation of the cuticle. However, the detailed evolutionary processes of S100A3 and PAD3 during mammalian evolution are unknown. Here, we show that ...
Source: Gene - July 11, 2019 Category: Genetics & Stem Cells Authors: Minato T, Unno M, Kitano T Tags: Gene Source Type: research

Compound heterozygous mutations in SMO associated with anterior segment dysgenesis and morning glory syndrome.
Abstract Eye development in vertebrates is a highly coordinated multistep process while defects in key factors might lead to severe congenital ocular disorders. SMO encodes a G protein-coupled receptor that functions in Hedgehog signal transduction, an essential step during eye development. Here we reported the first identification of compound heterozygous mutations (c.G338A; p.R113Q and c.C1619T; p.A540V) in the SMO gene in a patient with both anterior segment dysgenesis (congenital corneal opacity, cataract) and morning glory syndrome, using trio-based whole exome sequencing. The clinical manifestations of this ...
Source: Gene - July 10, 2019 Category: Genetics & Stem Cells Authors: Zhang J, Li Y, Fan Y, Wu D, Xu J Tags: Gene Source Type: research

Overcoming multiple drug resistance in lung cancer using siRNA targeted therapy.
Abstract Among cancers, lung cancer is the most morbidity and mortality disease that is remaining the fatalist. Generally, there are multiple treatment procedures for lung cancer, such as surgery, immunotherapy, radiotherapy and chemotherapy. There is, therefore, an urgent need for more specified and efficient methods for treatment of lung cancer such as RNAi, which in combination with traditional therapies could silence genes that are involved in the drug resistance. These genes may either be motivators of apoptosis inhibition, EMT and DNA repair system promoters or a member of intracellular signaling pathways, s...
Source: Gene - July 10, 2019 Category: Genetics & Stem Cells Authors: Naghizadeh S, Mohammadi A, Baradaran B, Mansoori B Tags: Gene Source Type: research

A SEPALLATA1-like gene of Isatis indigotica Fort. regulates flowering time and specifies floral organs.
Abstract An orthologous gene of SEPALLATA1, designated as IiSEP1, was isolated from Isatis indigotica. The genomic DNA of IiSEP1 is 3.1 Kb in length. The full-length cDNA of IiSEP1 is 1481 bp and contains a 756 bp ORF encoding a 251-amino-acid protein. Sequence comparison revealed that IiSEP1 belongs to the MADS-box gene family. IiSEP1 contains 7 exons and 6 introns, showing similar exon-intron structure with Arabidopsis SEP1. Phylogenetic analysis suggested that IiSEP1 belonged to AGL2/SEP subfamily and was likely to be an I. indigotica ortholog of Arabidopsis SEP1. Quantitative real-time PCR showed that IiSE...
Source: Gene - July 10, 2019 Category: Genetics & Stem Cells Authors: Ma YQ, Pu ZQ, Zhang L, Lu MX, Zhu Y, Hao CY, Xu ZQ Tags: Gene Source Type: research