Cloning and characterization of heat shock transcription factor 1 and its functional role for Hsp70 production in the sea slug Onchidium reevesii
This study demonstrated that OrHSF1 regulates Hsp70 expression in marine mollusks under low-frequency noise, and HSF1-Hsp70 axis plays a key role in stress response.PMID:38381511 | DOI:10.1016/j.gene.2023.147945 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Zhihan Tu Liusiqiao Tang Mohamed H Abo-Raya Mengying Sun Heding Shen Youji Wang Source Type: research
ChIP-Seq analysis reveals PRKACB as a target gene of HOXC13 involved in rabbit hair follicle development
In conclusion, our research revealed that PRKACB has the potential to serve as a novel target gene of HOXC13, contributing to the regulation of the proliferation and apoptosis of DPCs. The process of identifying global target genes can contribute to the understanding of the intricate pathways that HOXC13 regulates in the growth of HFs.PMID:38381512 | DOI:10.1016/j.gene.2023.147946 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Yang Chen Zhiyuan Bao Fan Yao Yan Liu Bohao Zhao Xinsheng Wu Source Type: research
Corrigendum to: "Identification of SLC12A8 as a valuable prognostic biomarker and immunotherapeutic target by comprehensive pan-cancer analysis" [Gene 903 (2024) 148211]
Gene. 2024 Feb 20;908:148267. doi: 10.1016/j.gene.2024.148267. Online ahead of print.NO ABSTRACTPMID:38382297 | DOI:10.1016/j.gene.2024.148267 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Dongmin Yu Hanhongfeng Ma Deping Li Huiyang Tang Wei Li Meifang Li Source Type: research
Phenotypic and genotypic analysis of a patient with Miyoshi myopathy caused by truncated protein
We report a novel pathogenic mutation in DYSF gene with Miyoshi myopathy and demonstrate this variant causes skipping of exon15 by minigene splicing assay. We point out the need of conducting functional analysis to verify the pathogenicity of intronic mutation. The finding enriches the mutation spectrum of DYSF gene and laid a foundation for future studies on the correlation between genotype and phenotype.PMID:38381504 | DOI:10.1016/j.gene.2023.147929 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Jinyi Zhou Rui Zhou Qihua Feng Xiaoxiang Song Xuqin Chen Source Type: research
Low-concentration exogenous 3-indoleacetic acid improves fruit-setting rate of Marsdenia tenacissima by inhibiting the expression of embryo abortion-related genes
In this study, we conducted a field experiment to determine the fruit-setting rate, seed-setting rate, fruit size, and changes in transcriptional expression of related genes by spraying 10 and 50 mg·L-1 of 3-indoleacetic acid (IAA). The control plants were sprayed with distilled water. Our results indicated that the fruit-setting rate was 0.15 when treated with 10 mg·L-1 of IAA, which was 2.76-fold higher than that of the control. Compared with that of the control, the number of differentially expressed genes (DEGs) regulated by 10 mg·L-1 of IAA was 28.6-fold higher than that regulated by 50 mg·L-1 of IAA. These DEGs w...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Haoyue Zhang Jiuxia Zhao Jingling Zhang Shuhan Wen Shiqing Xie Shengchao Yang Junwen Chen Yanli Zhou Guangqiang Long Source Type: research
Inhibiting neddylation with MLN4924 potentiates hypoxia-induced apoptosis of mouse type B spermatogonia GC-2 cells
In this study, aiming to explore the role of neddylation in male infertility, we used the specific neddylation inhibitor MLN4924 for treatment in mouse type B spermatogonia GC-2 cells. Our results showed that MLN4924 had no apparent effect on GC-2 cell apoptosis under normoxia, but significantly increased apoptotic cells under hypoxia. Transcriptomic analysis and qPCR assay confirmed that MLN4924 could suppress the expression of hypoxia target genes in GC-2 cells under hypoxia. In addition, MLN4924 could enhance the induction of intracellular and mitochondrial reactive oxygen species (ROS) under hypoxia. These results indi...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Zhu Chen Zixuan Wang Chunchun Zhu Hongyan Deng Xiaoyun Chen Source Type: research
Development and genetic regulation of pollen intine in Arabidopsis and rice
Gene. 2024 Jan 30;893:147936. doi: 10.1016/j.gene.2023.147936. Epub 2023 Oct 29.ABSTRACTPollen intine serves as a protective layer situated between the pollen exine and the plasma membrane. It performs essential functions during pollen development, including maintaining the morphological structure of the pollen, preventing the loss of pollen contents, and facilitating pollen germination. The formation of the intine layer commences at the bicellular pollen stage. Pectin, cellulose, hemicellulose and structural proteins are the key constituents of the pollen intine. In Arabidopsis and rice, numerous regulatory factors associ...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Zaibao Zhang Mengke Sun Tao Xiong Fan Ye Ziwei Zhao Source Type: research
Population genetics provides insights into the important agronomic traits of Ganoderma cultivation varieties in China
This study aimed to investigate the species diversity and genetic differentiation of the genome of the main cultivated strains of Ganoderma in China. Population genomics analysis was conducted based on 150 cultivated strains of Ganoderma collected nationwide. The results indicated that the main species currently cultivated in China were Ganoderma sichuanense and Ganoderma lucidum, with a minor proportion of Ganoderma sessile, Ganoderma weberianum, Ganoderma sinense, Ganoderma gibbosum and Ganoderma australe. A total of 336,506 high-quality single nucleotide polymorphism (SNP) loci were obtained through population evolution...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Lei Sun Shi-da Li Yin Li Lei Wang Xiu-Min Pu Yu-Peng Ge Qin Na Wei-Huan Li Xian-Hao Cheng Source Type: research
Diosgenin biosynthesis investigation in medicinal herb (Tribulus terrestris) by transcriptome analysis
Gene. 2024 Jan 30;893:147937. doi: 10.1016/j.gene.2023.147937. Epub 2023 Oct 29.ABSTRACTNext-generation sequencing (NGS) has revolutionized the analysis of specific genes, pathways, and their regulation in various species. Tribulus terrestris L., an annual medicinal herb of Zygophyllaceae family, has gained significant attention due to its diverse medicinal properties, including anti-inflammatory, antimicrobial, and anti-cancer effects. Diosgenin, a steroidal saponin, is the major bioactive compound responsible for the medicinal importance of T. terrestris. However, there is a paucity of information regarding the genes inv...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Parul Tyagi Mrinalini Prasad Shivangi Mathur Rajiv Ranjan Source Type: research
RNA-seq transcriptome analysis provides new insights into the negative effects of tannic acid on the intestinal function of Brandt's voles (Lasiopodomys brandtii)
In this study, male Brandt's vole (13 weeks old) were divided into two groups and given 0 (control) or 1,200 (TA-treated) mg•kg-1 TA for 18 days. Then RNA sequencing was used to conduct a thorough transcriptome analysis on the duodenum, jejunum, and ileum of Brandt's voles. Results showed that TA significantly increased serum total cholesterol concentration (P < 0.05) and decreased the nutrient digestibility (P < 0.05) of Brandt's voles. Furthermore, there were 174 differentially expressed genes (DEGs) in the duodenum, 96 DEGs in the jejunum, and 88 DEGs in the ileum between the control and TA-treated groups. Enric...
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Minghui Gu Yuxuan Han Xin Dai Xuwei Ma Weiwei Ge Wanhong Wei Shengmei Yang Source Type: research
Cloning and characterization of heat shock transcription factor 1 and its functional role for Hsp70 production in the sea slug Onchidium reevesii
This study demonstrated that OrHSF1 regulates Hsp70 expression in marine mollusks under low-frequency noise, and HSF1-Hsp70 axis plays a key role in stress response.PMID:38381511 | DOI:10.1016/j.gene.2023.147945 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Zhihan Tu Liusiqiao Tang Mohamed H Abo-Raya Mengying Sun Heding Shen Youji Wang Source Type: research
ChIP-Seq analysis reveals PRKACB as a target gene of HOXC13 involved in rabbit hair follicle development
In conclusion, our research revealed that PRKACB has the potential to serve as a novel target gene of HOXC13, contributing to the regulation of the proliferation and apoptosis of DPCs. The process of identifying global target genes can contribute to the understanding of the intricate pathways that HOXC13 regulates in the growth of HFs.PMID:38381512 | DOI:10.1016/j.gene.2023.147946 (Source: Gene)
Source: Gene - February 21, 2024 Category: Genetics & Stem Cells Authors: Yang Chen Zhiyuan Bao Fan Yao Yan Liu Bohao Zhao Xinsheng Wu Source Type: research
Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis
In conclusion, our findings reveal a new regulatory mechanism for circ_0000119, which sponges the miR-502-5p, suppresses MTBP expression, and finally promotes GC progression.PMID:38378131 | DOI:10.1016/j.gene.2024.148296 (Source: Gene)
Source: Gene - February 20, 2024 Category: Genetics & Stem Cells Authors: Mengyan Xie Yunru Gu Tingting Xu Xinming Jing Yongqian Shu Source Type: research
SUPT5H mutations associated with elevation of Hb A < sub > 2 < /sub > level: Identification of two novel variants and literature review
In this study, we identified two novel variants in the SUPT5H gene: a frameshift mutation (SUPT5H: c.3032_3033delTG, p.M1011Mfs*9) and a nonsense mutation (SUPT5H: c.397C > T, p.Arg133*) in two Chinese individuals. Utilizing a combination of phenotype analysis, bioinformatics analysis, and functional analysis, we deduced that these two variants modified the SUPT5H protein's structure, thereby impacting its function and consequently leading to the heightened Hb A2 level phenotype found in the carriers. Furthermore, through a comprehensive literature review, a mutation spectrum was consolidated for SUPT5H, an investigatio...
Source: Gene - February 19, 2024 Category: Genetics & Stem Cells Authors: Zezhang Lin Xiongda Liang Xiaofeng Wei Guanxia Liang Dina Zhu Hongting Xie Tizhen Yan Xuan Shang Source Type: research
E-cadherin re-expression: Its potential in combating TRAIL resistance and reversing epithelial-to-mesenchymal transition
Gene. 2024 Feb 17:148293. doi: 10.1016/j.gene.2024.148293. Online ahead of print.ABSTRACTThe major limitation of conventional chemotherapy drugs is their lack of specificity for cancer cells. As a selective apoptosis-inducing agent, tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) has emerged as an attractive alternative. However, most of the cancer cells are found to be either intrinsically resistant to the TRAIL protein or may develop resistance after multiple treatments, and TRAIL resistance can induce epithelial-to-mesenchymal transition (EMT) at a later stage, promoting cancer invasion and migrati...
Source: Gene - February 19, 2024 Category: Genetics & Stem Cells Authors: Ser Hui San Siew Ching Ngai Source Type: research
