Identification of suitable reference genes for RT-qPCR studies in human parathyroid tissue glandular cells
This study aims to determine and compare the most stable to least stable genes for parathyroid tissue cells. 43 human parathyroid tissue obtained from primary and secondary hyperparathyroidism patients and glandular cells isolated enzymatically by removal of extracellular matrix components. After extraction of the total RNA, cDNA synthesis was performed, then qRT-PCR evaluated 14 candidate reference genes. Stability was determined by RefFinder software (Delta ct, BestKeeper, Genorm, and NormFinder algorithms), and the outcome was evaluated for five groups. Even if assessed with different groups, the most stable genes were ...
Source: Gene - March 15, 2024 Category: Genetics & Stem Cells Authors: Beyza Goncu Source Type: research
Revisiting the role and mechanism of ELF3 in circadian clock modulation
Gene. 2024 Mar 13:148378. doi: 10.1016/j.gene.2024.148378. Online ahead of print.ABSTRACTThe gene encoding EARLY FLOWERING3 (ELF3) is necessary for photoperiodic flowering and the normal regulation of circadian rhythms. It provides important information at the cellular level to uncover the biological mechanisms that improve plant growth and development. ELF3 interactions with transcription factors such as BROTHER OF LUX ARRHYTHMO (BOA), LIGHT-REGULATED WD1 (LWD1), PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), PHYTOCHROME-INTERACTING FACTOR 7 (PIF7), and LUX ARRHYTHMO (LUX) suggest a role in evening complex (EC) independent path...
Source: Gene - March 15, 2024 Category: Genetics & Stem Cells Authors: Xingzhun Zhu Hongtao Wang Source Type: research
Polymorphism in the leucine-rich repeats of TLR7 in different breeds of chicken and in silico analysis of its effect on TLR7 structure and function
This study first aimed to compare the single nucleotide polymorphisms (SNPs) associated with the ligand binding site of TLR7 in three indigenous chicken breeds namely Aseel, Kadaknath, Nicobari along with an exotic breed White Leghorn. Four synonymous SNPs (P123P, I171I, N339N and L421L) and four non-synonymous SNPs (I121V, S135T, F356S and S447G) were identified among various breeds. We employed in silico tools to screen the pathogenic nsSNPs and one nsSNP was identified as having potential impact on chTLR7 protein. Moreover, sequence and structure-based methods were used to determine the effect of nsSNPs on protein stabi...
Source: Gene - March 15, 2024 Category: Genetics & Stem Cells Authors: Abinaya Kaliappan Saravanan Ramakrishnan Prasad Thomas Surya Kant Verma Khushboo Panwar Mithilesh Singh Sohini Dey Madhan Mohan Chellappa Source Type: research
ZEB2 alleviates Hirschsprung's-associated enterocolitis by promoting the proliferation and differentiation of enteric neural precursor cells via the Notch-1/Jagged-2 pathway
CONCLUSION: Overexpression of ZEB2 ameliorates LPS-induced ENPCs' dysfunction via the Notch-1/Jagged-2 pathway, thus playing a role in HAEC.PMID:38485033 | DOI:10.1016/j.gene.2024.148365 (Source: Gene)
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Yong Feng Chonggao Zhou Fan Zhao Tidong Ma Yong Xiao Kun Peng Renpeng Xia Source Type: research
Metagenomic analysis of intestinal microbial function and key genes responsive to acute high-salinity stress in Nile tilapia (Oreochromis niloticus)
Gene. 2024 Mar 12:148371. doi: 10.1016/j.gene.2024.148371. Online ahead of print.ABSTRACTThe intestinal microbiota is increasingly recognized as playing an important role in aquatic animals. To investigate the functional roles and mechanisms of the intestinal microbial genes/enzymes responding to salinity stress or osmotic pressure in fish, metagenomic analysis was carried out to evaluate the response of intestinal microbiota and especially their functional genes/enzymes from freshwater (the control group) to acute high salinity stress (the treatment group) in Nile tilapia. Our results showed that at the microbial communit...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Jiayi Gong Fengmeng Xu Yao Li Yiyong He Zhizheng Liang Xiao Chen Xiaoyong Zhang Li Liu Lei Zhou Xiande Huang Source Type: research
Nigella sativa, Anthemis hyaline and Citrus sinensis extracts reduce SARS-CoV-2 replication by fluctuating Rho GTPase, PI3K-AKT, and MAPK/ERK pathways in HeLa-CEACAM1a cells
This study aimed to investigate the influence of Anthemis hyaline, Nigella sativa, and Citrus sinensis extracts on coronavirus replication and apoptosis-related pathways. HeLa-CEACAM1a cells were exposed to mouse hepatitis virus-A59. After viral inoculation, the mRNA levels of 36 genes were quantified using a Fluidigm Dynamic Array nanofluidic chip. IL-8 level and intracellular Ca2+ concentration was measured, and viral titer was assessed by the TCID50/ml assay to detect the extent of infection. Treatment with Nigella sativa extract surged the inflammatory cytokine IL-8 level at both 24 and 48-hour. Changes in gene express...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Mehmet Bostanc ıklıoğlu Mehri İğci Mustafa Ula şlı Source Type: research
Analysis and validation of hub genes in neutrophil extracellular traps for the long-term prognosis of myocardial infarction
CONCLUSION: The NETs formation could be highly related to MI and the long-term prognosis of MI can be significantly influenced by the NETs formation. Four hub genes, namely FOS, EGR1, PTGS2, and HIST1H4H, have the potential to be key genes related to this process. They could also serve as biomarkers for predicting MI prognosis and as targets for gene therapy.PMID:38485036 | DOI:10.1016/j.gene.2024.148369 (Source: Gene)
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Xuan Li Wenyu Wu Huan He Lin Guan Guancheng Chen Zhijun Lin Huan Li Jialin Jiang Xin Dong Zhuoji Guan Pinliang Chen Zigang Pan Weiwei Huang Runjia Yu Wenxin Song Lu Lu Zhongqi Yang Zixin Chen Lingjun Wang Shaoxiang Xian Jie Chen Source Type: research
Research progress of RP1L1 gene in disease
Gene. 2024 Mar 12:148367. doi: 10.1016/j.gene.2024.148367. Online ahead of print.ABSTRACTRetinitis pigmentosa 1-like 1 (RP1L1) is a component of photoreceptor cilia. Pathogenic variants in RP1L1 cause photoreceptor diseases, suggesting that RP1L1 plays an important role in photoreceptor biology, although its exact function is unknown. To date, RP1L1 variants have been associated with occult macular dystrophy (cone degeneration) and retinitis pigmentosa (rod disease). Here, we summarize the reported RP1L1-associated photoreceptor pathogenic mutations. The association between RP1L1 and other diseases (mainly several tumors) ...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Jiali Liu Melvin R Hayden Ying Yanga Source Type: research
A review of the advances, insights, and prospects of gene therapy for Alzheimer's disease: A novel target for therapeutic medicine
Gene. 2024 Mar 12:148368. doi: 10.1016/j.gene.2024.148368. Online ahead of print.ABSTRACTNeurodegenerative diseases such as Alzheimer's disease (AD) are still an important issue for scientists because it is difficult to cure with the available molecular medications and conventional treatments. Due to the complex nature of the brain structures and heterogeneous morphological and physiological properties of neuronal cells, interventions for cerebral-related disorders using surgical approaches, and classical and ongoing treatments remain hard for physicians. Furthermore, the development of newly designed medications attempts ...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Bahar Ataei Mahsa Hokmabadi Sahar Asadi Elnaz Asadifard Seyed Mohsen Aghaei Zarch Sajad Najafi Saeid Bagheri-Mohammadi Source Type: research
ZEB2 alleviates Hirschsprung's-associated enterocolitis by promoting the proliferation and differentiation of enteric neural precursor cells via the Notch-1/Jagged-2 pathway
CONCLUSION: Overexpression of ZEB2 ameliorates LPS-induced ENPCs' dysfunction via the Notch-1/Jagged-2 pathway, thus playing a role in HAEC.PMID:38485033 | DOI:10.1016/j.gene.2024.148365 (Source: Gene)
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Yong Feng Chonggao Zhou Fan Zhao Tidong Ma Yong Xiao Kun Peng Renpeng Xia Source Type: research
Metagenomic analysis of intestinal microbial function and key genes responsive to acute high-salinity stress in Nile tilapia (Oreochromis niloticus)
Gene. 2024 Mar 12:148371. doi: 10.1016/j.gene.2024.148371. Online ahead of print.ABSTRACTThe intestinal microbiota is increasingly recognized as playing an important role in aquatic animals. To investigate the functional roles and mechanisms of the intestinal microbial genes/enzymes responding to salinity stress or osmotic pressure in fish, metagenomic analysis was carried out to evaluate the response of intestinal microbiota and especially their functional genes/enzymes from freshwater (the control group) to acute high salinity stress (the treatment group) in Nile tilapia. Our results showed that at the microbial communit...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Jiayi Gong Fengmeng Xu Yao Li Yiyong He Zhizheng Liang Xiao Chen Xiaoyong Zhang Li Liu Lei Zhou Xiande Huang Source Type: research
Nigella sativa, Anthemis hyaline and Citrus sinensis extracts reduce SARS-CoV-2 replication by fluctuating Rho GTPase, PI3K-AKT, and MAPK/ERK pathways in HeLa-CEACAM1a cells
This study aimed to investigate the influence of Anthemis hyaline, Nigella sativa, and Citrus sinensis extracts on coronavirus replication and apoptosis-related pathways. HeLa-CEACAM1a cells were exposed to mouse hepatitis virus-A59. After viral inoculation, the mRNA levels of 36 genes were quantified using a Fluidigm Dynamic Array nanofluidic chip. IL-8 level and intracellular Ca2+ concentration was measured, and viral titer was assessed by the TCID50/ml assay to detect the extent of infection. Treatment with Nigella sativa extract surged the inflammatory cytokine IL-8 level at both 24 and 48-hour. Changes in gene express...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Mehmet Bostanc ıklıoğlu Mehri İğci Mustafa Ula şlı Source Type: research
Analysis and validation of hub genes in neutrophil extracellular traps for the long-term prognosis of myocardial infarction
CONCLUSION: The NETs formation could be highly related to MI and the long-term prognosis of MI can be significantly influenced by the NETs formation. Four hub genes, namely FOS, EGR1, PTGS2, and HIST1H4H, have the potential to be key genes related to this process. They could also serve as biomarkers for predicting MI prognosis and as targets for gene therapy.PMID:38485036 | DOI:10.1016/j.gene.2024.148369 (Source: Gene)
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Xuan Li Wenyu Wu Huan He Lin Guan Guancheng Chen Zhijun Lin Huan Li Jialin Jiang Xin Dong Zhuoji Guan Pinliang Chen Zigang Pan Weiwei Huang Runjia Yu Wenxin Song Lu Lu Zhongqi Yang Zixin Chen Lingjun Wang Shaoxiang Xian Jie Chen Source Type: research
Research progress of RP1L1 gene in disease
Gene. 2024 Mar 12:148367. doi: 10.1016/j.gene.2024.148367. Online ahead of print.ABSTRACTRetinitis pigmentosa 1-like 1 (RP1L1) is a component of photoreceptor cilia. Pathogenic variants in RP1L1 cause photoreceptor diseases, suggesting that RP1L1 plays an important role in photoreceptor biology, although its exact function is unknown. To date, RP1L1 variants have been associated with occult macular dystrophy (cone degeneration) and retinitis pigmentosa (rod disease). Here, we summarize the reported RP1L1-associated photoreceptor pathogenic mutations. The association between RP1L1 and other diseases (mainly several tumors) ...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Jiali Liu Melvin R Hayden Ying Yanga Source Type: research
A review of the advances, insights, and prospects of gene therapy for Alzheimer's disease: A novel target for therapeutic medicine
Gene. 2024 Mar 12:148368. doi: 10.1016/j.gene.2024.148368. Online ahead of print.ABSTRACTNeurodegenerative diseases such as Alzheimer's disease (AD) are still an important issue for scientists because it is difficult to cure with the available molecular medications and conventional treatments. Due to the complex nature of the brain structures and heterogeneous morphological and physiological properties of neuronal cells, interventions for cerebral-related disorders using surgical approaches, and classical and ongoing treatments remain hard for physicians. Furthermore, the development of newly designed medications attempts ...
Source: Gene - March 14, 2024 Category: Genetics & Stem Cells Authors: Bahar Ataei Mahsa Hokmabadi Sahar Asadi Elnaz Asadifard Seyed Mohsen Aghaei Zarch Sajad Najafi Saeid Bagheri-Mohammadi Source Type: research
