Bitter orange peel extract induces endoplasmic reticulum-mediated autophagy in human hepatoma cells
This study was aimed at investigating the anti-cancer effect of bitter orange peel extract (BOPE) on Hep3B, a human hepatoma cell line. Upon treatment of Hep3B cells with 6.25–100 μg/mL of BOPE for 72 h, its antiproliferative effect was shown to increase in a concentration- and time-dependent manner. The observations, such as concentration-dependent elevation of LC3 fluorescent puncta, increased conversion of LC3-I to LC3-II, no characteristic apoptotic nuclear fragmentation, and no significant effect on percentages of annexin V-positive apoptotic cells, confirmed the autophagy triggered by BOPE in Hep3B cells. More...
Source: Journal of Functional Foods - June 15, 2019 Category: Nutrition Source Type: research
Danhong Huayu Koufuye prevents venous thrombosis through antiinflammation via Sirtuin 1/NF-κB signaling pathway
ConclusionDHK may prevent DVT by inhibiting inflammation via SIRT1/NF-κB signaling pathway.Graphical abstract (Source: Journal of Ethnopharmacology)
Source: Journal of Ethnopharmacology - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
Human TRPA1 activation by terpenes derived from the essential oil of daidai, Citrus aurantium L. var. daidai Makino.
Abstract
Daidai (bitter orange, Citrus aurantium) is characterized by its fresh citrus scent. In Japanese cuisine, its juice is an important ingredient. As tons of industrial waste is obtained while processing the daidai juice, additional utilization of this waste has great social value. In our study, we prepared the essential oil from the waste obtained during daidai juice processing and demonstrated that the oil activates human TRPA1 (hTRPA1). This oil contains 10 types of terpenes, all of which activated hTRPA1 with an EC50 value of 6-167 µM. To our knowledge, this study is the first to show a hTRPA1 ...
Source: Bioscience, Biotechnology, and Biochemistry - May 9, 2019 Category: Biochemistry Authors: Terada Y, Yamashita R, Ihara N, Yamazaki-Ito T, Takahashi Y, Masuda H, Sakuragawa S, Ito S, Ito K, Watanabe T Tags: Biosci Biotechnol Biochem Source Type: research
Statistical modeling of the inactivation of spoilage microorganisms during ohmic heating of sour orange juice
This study sought to investigate the effect of four ohmic resistance variables, temperature (Temp), voltage (V), current (AMP) and electric conductivity (EC), on the population of two yeasts, an ascospore and three fermentative bacteria, by inoculation into sour orange juice. The incidence of the variables was evaluated through different regression models. The results of the simple linear regression (SLR) indicated that Temp, AMP and EC had a significant negative effect on the population of all microorganisms, while V had no effect on the population of any microorganism. The results of the stepwise linear regression (SWLR)...
Source: LWT Food Science and Technology - April 26, 2019 Category: Food Science Source Type: research
Tangshen Formula Alleviates Hepatic Steatosis by Inducing Autophagy Through the AMPK/SIRT1 Pathway
Conclusion
In conclusion, the present study demonstrated that autophagy was involved in relieving the effects of TSF against NAFLD, which were mediated by the AMPK/SIRT1 pathway (Figure 7D). These findings may improve our current understanding of the role of TSF in treating hepatic steatosis and provide an experimental basis for the clinical application of TSF in NAFLD and its related metabolic syndrome.
Ethics Statement
This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Ethics Co...
Source: Frontiers in Physiology - April 25, 2019 Category: Physiology Source Type: research
In vitro Susceptibility and Evaluation of Techniques for Understanding the Mode of Action of a Promising Non-antibiotic Citrus Fruit Extract Against Several Pathogens
Conclusion
This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the vi...
Source: Frontiers in Microbiology - April 23, 2019 Category: Microbiology Source Type: research
Analysis of the cytotoxic, genotoxic, mutagenic, and pro-oxidant effect of synephrine, a component of thermogenic supplements, in human hepatic cells in vitro.
In conclusion, SN showed no cytotoxic, genotoxic, and mutagenic potential at relevant concentrations for thermogenic users in human hepatic cells in vitro, although, it plays pro-oxidative action, and cytostatic effects. Taken together, our results suggest that other investigations about the hazard absence of this thermogenic compound should be performed.
PMID: 31004705 [PubMed - as supplied by publisher] (Source: Toxicology)
Source: Toxicology - April 16, 2019 Category: Toxicology Authors: Ribeiro DL, Machado ART, da Silva Machado C, Dos Santos PW, Aissa AF, Barcelos GRM, Antunes LMG Tags: Toxicology Source Type: research
Molecules, Vol. 24, Pages 1270: In Vitro Activity of Essential Oils against Saprolegnia parasitica
Mancianti
Saprolegnia spp. water molds severely impact fish health in aquaculture, fish farms and hobby fish tanks colonizing mature and immature stages of fishes, as well as eggs. Considering that there are no drugs licensed for treating and/or control the organism, efficient and environmental low-impact methods to control these oomycetes in aquaculture are needed. The aim of the present report was to evaluate the in vitro sensitivity of Saprolegnia parasitica to essential oils (EOs) from Citrus aurantium L., Citrus bergamia Risso et Poiteau, Citrus limon Burm. f., Citrus paradisi Macfad, Citrus sinensis Osbeck, Cinna...
Source: Molecules - March 31, 2019 Category: Chemistry Authors: Simona Nardoni Basma Najar Baldassare Fronte Luisa Pistelli Francesca Mancianti Tags: Article Source Type: research
Antibacterial Activity of Polymeric Nanofiber Membranes Impregnated with Texas Sour Orange Juice
Publication date: Available online 7 March 2019Source: European Polymer JournalAuthor(s): Mandana Akia, Cristobal Rodriguez, Luis Materon, Robert Gilkerson, Karen LozanoAbstractThe antibacterial activity of Nylon 6 nanofiber membranes and commercial gauze pads dipped in Texas sour orange juice were evaluated. The Nylon 6 nanofiber mats were developed using a centrifugal spinning technique. The resultant mats were composed of fibers with average diameter of 209 nm, while the average fiber diameter of the commercial gauze pad was 8.73 µm. The dipping solution was composed of 100% Texas sour orange juice. The samples were im...
Source: European Polymer Journal - March 7, 2019 Category: Chemistry Source Type: research
Review of Published Bitter Orange Extract and p-Synephrine Adverse Event Clinical Study Case Reports.
Authors: Stohs SJ, Ray SD
Abstract
p-Synephrine is the primary active ingredient in bitter orange (Citrus aurantium) extract and is present in other citrus species. This review summarizes all known case reports that have been published regarding adverse events associated with multi-ingredient dietary supplements containing bitter orange extract. A common characteristic of all the case studies was the assumption that if bitter orange extract is listed on the label of the product it is the most likely cause of any adverse effect, although in no case was the presence of p-synephrine determined or a direct lin...
Source: Journal of Dietary Supplements - March 7, 2019 Category: Nutrition Tags: J Diet Suppl Source Type: research
Molecules, Vol. 24, Pages 908: Essential Oil Composition and Biological Activity of “Pompia”, a Sardinian Citrus Ecotype
Luisa Pistelli
Pompia is a Sardinian citrus ecotype whose botanical classification is still being debated. In the present study, the composition of Pompia peel essential oil (EO) is reported for the first time, along with that of the leaf EO, as a phytochemical contribution to the classification of this ecotype. The peel EO was tested for its antioxidant ability (with both the 2,2-diphenyl-1-picarylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays). Moreover, its antimicrobial activities were tested for the first time on dermatophytes (Microsporum canis, Microsporum gypseum, and Trichophyton mentagro...
Source: Molecules - March 4, 2019 Category: Chemistry Authors: Guido Flamini Laura Pistelli Simona Nardoni Valentina Virginia Ebani Angela Zinnai Francesca Mancianti Roberta Ascrizzi Luisa Pistelli Tags: Article Source Type: research
Preventive Effect of Citrus aurantium Peel Extract on High-Fat Diet-Induced Non-alcoholic Fatty Liver in Mice.
Abstract
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic lipid accumulation, which is the most common form of chronic liver disease. Multiple clinical studies using natural compounds such as flavonoids have been conducted to treat NAFLD. In the present study, the pharmacological effect of Citrus aurantium L. (Rutaceae) peel extract (CAE), which contains over 27% of polymethoxyflavone nobiletin, on NAFLD was evaluated using a high-fat diet (HFD) animal model susceptible to developing NAFLD. C57BL/6 mice were fed an HFD (60% kcal of energy derived from fat) for 8 weeks to induce obesity....
Source: Biological and Pharmaceutical Bulletin - February 6, 2019 Category: Drugs & Pharmacology Authors: Han HY, Lee SK, Choi BK, Lee DR, Lee HJ, Kim TW Tags: Biol Pharm Bull Source Type: research
Citrus Aurantium and caffeine complex versus placebo on biomarkers of metabolism: a double blind crossover design
The purpose of this study was to examine resting the metabolic response to the ingestion of a complex containing Citrus Aurantium + Caffeine (CA + C) and if its consumption influences metabolic recovery follow... (Source: Journal of the International Society of Sports Nutrition)
Source: Journal of the International Society of Sports Nutrition - February 6, 2019 Category: Sports Medicine Authors: Brian Kliszczewicz, Emily Bechke, Cassie Williamson, Zackery Green, Paul Bailey, John McLester and Cherilyn McLester Tags: Research article Source Type: research
Evidence for the efficacy and safety of herbal weight loss preparations
Publication date: Available online 30 January 2019Source: Journal of Integrative MedicineAuthor(s): Rachael Farrington, Ian F. Musgrave, Roger W. ByardAbstractRising rates of obesity across the globe have been associated with an increase in the use of herbal preparations for weight control. However, the mechanisms of action for these substances are often not known, as is the potential for interaction with other herbal preparations or prescription pharmaceutical drugs. To investigate the reported efficacy and safety of herbal weight loss preparations, we conducted a review of the literature focusing on herbs that are most c...
Source: Journal of Integrative Medicine - January 31, 2019 Category: Complementary Medicine Source Type: research
Clinical Pharmacology of Citrus aurantium and Citrus sinensis for the Treatment of Anxiety.
Conclusions: Overview of clinical trials conducted with Citrus aurantium or Citrus sinensis on people with anxiety showed that inhalation or oral administration of Citrus aurantium and inhalation of Citrus sinensis can exert beneficial effects on anxiety; however, because of incomplete accuracy in the reporting of methodology, further more complete clinical studies are warranted.
PMID: 30622597 [PubMed] (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - January 10, 2019 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
