Downregulation of RPS15A by miR-29a-3p attenuates cell proliferation in colorectal carcinoma.
In this study, we found that miR-29a-3p was at lower level expression in CRC tissues and cell lines. Experimental up-regulation miR-29a-3p with mimic could inhibit cell proliferation, but induced cell cycle arrest at G0/G1 phase and apoptosis in CRC cells. MiR-29a-3p overexpression significantly down-regulated the expression levels of CDK4, Cyclin D1, and Bax, but up-regulated the expression levels of p21 and Bcl-2 in DLD-1 cells. Moreover, ribosomal protein S15A (RPS15A) was predicted and confirmed as a direct target gene of miR-29a-3p. Furthermore, restoration of RPS15A could rescue the phenotypic changes caused by miR-2...
Source: Bioscience, Biotechnology, and Biochemistry - July 13, 2019 Category: Biochemistry Authors: Zheng Z, Cui H, Wang Y, Yao W Tags: Biosci Biotechnol Biochem Source Type: research

Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97.
Abstract Frigocyclinone is a novel antibiotic with antibacterial and anticancer activities. It is produced by both Antarctica-derived Streptomyces griseus NTK 97 and marine sponge-associated Streptomyces sp. M7_15. Here, we first report the biosynthetic gene cluster of frigocyclinone in the S. griseus NTK 97. The frigocyclinone gene cluster spans a DNA region of 33-kb which consists of 30 open reading frames (ORFs), encoding minimal type II polyketide synthase, aromatase and cyclase, redox tailoring enzymes, sugar biosynthesis-related enzymes, C-glycosyltransferase, a resistance protein, and three regulatory prote...
Source: Bioscience, Biotechnology, and Biochemistry - July 13, 2019 Category: Biochemistry Authors: Mo J, Ye J, Chen H, Hou B, Wu H, Zhang H Tags: Biosci Biotechnol Biochem Source Type: research

Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host.
Abstract A low-temperature protein expression system is useful for the production of thermolabile proteins. We previously developed a system that enables constitutive protein production at low temperatures, using the psychrotrophic bacterium Shewanella livingstonensis Ac10 as the host. To increase the utility of this system, in the present study, we introduced a repressible promoter of the trp operon of this bacterium into the system. When ß-lactamase was produced under the control of this promoter at 18°C and 4°C, the yields were 75 and 33 mg/L-culture, respectively, in the absence of L-Trp, and the...
Source: Bioscience, Biotechnology, and Biochemistry - July 11, 2019 Category: Biochemistry Authors: Kawai S, Kawamoto J, Ogawa T, Kurihara T Tags: Biosci Biotechnol Biochem Source Type: research

Characterization of saponins and phenolic compounds: antioxidant activity and inhibitory effects on α-glucosidase in different varieties of colored quinoa (Chenopodium quinoa Willd).
This study investigated the contents of saponins and phenolic compounds in relation to their antioxidant activity and α-glucosidase inhibition activity of 7 colored quinoa varieties. The total saponin content was significantly different among 7 varieties and ranged from 7.51 to 12.12 mg OAE/g DW. Darker quinoa had a higher content of phenolic compounds, as well as higher flavonoids and antioxidant activity than that of light varieties. Nine individual phenolic compounds were detected in free and bound form, with gallic acid and ferulic acid representing the major compounds. The free and bound phenolic compounds (gall...
Source: Bioscience, Biotechnology, and Biochemistry - July 7, 2019 Category: Biochemistry Authors: Han Y, Chi J, Zhang M, Zhang R, Fan S, Huang F, Xue K, Liu L Tags: Biosci Biotechnol Biochem Source Type: research

New approach to evaluating the effects of a drug on protein complexes with quantitative proteomics, using the SILAC method and bioinformatic approach.
Abstract Protein-protein interactions (PPIs) lead the formation of protein complexes that perform biochemical reactions that maintain the living state of the living cell. Although therapeutic drugs should influence the formation of protein complexes in addition to PPI network, the methodology analyzing such influences remain to be developed. Here, we demonstrate that a new approach combining HPLC (high performance liquid chromatography) for separating protein complexes, and the SILAC (stable isotope labeling using amino acids in cell culture) method for relative protein quantification, enable us to identify the pr...
Source: Bioscience, Biotechnology, and Biochemistry - July 7, 2019 Category: Biochemistry Authors: Kurokawa N, Kishimoto T, Tanaka K, Kondo J, Takahashi N, Miura Y Tags: Biosci Biotechnol Biochem Source Type: research

A herbivore-induced homoterpene volatile is emitted from Basella alba leaves.
Abstract An irregular C11 homoterpene, (E)-4,8-dimethylnona-1,3,7-triene (DMNT) was identified as a major component of the volatile compounds emitted from Basella alba leaves induced by herbivore. The terpenes including DMNT were not detected from the leaves infected by Botrytis cinerea. These results suggested that volatile emission from B. alba leaves was induced by herbivory but not by a fungal infection. PMID: 31280680 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - July 6, 2019 Category: Biochemistry Authors: Aboshi T, Toda A, Ashitani T, Murayama T Tags: Biosci Biotechnol Biochem Source Type: research

Downregulation of miR-145-5p elevates retinal ganglion cell survival to delay diabetic retinopathy progress by targeting FGF5.
In conclusion, our results demonstrated that inhibition of miR-145-5p might be a neuroprotective target for diabetes mellitus-related DR. Abbreviations: DR: diabetic retinopathy; RGCs: retinal ganglion cells; miR-145-5p: microRNA-145-5p; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; FGF: fibroblast growth factor; ATCC: American Type Culture Collection; WT: wild type; MUT: mutant type. PMID: 31272285 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - July 4, 2019 Category: Biochemistry Authors: Zhang J, Cui C, Xu H Tags: Biosci Biotechnol Biochem Source Type: research

Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique.
This study aimed to reveal the diversity of bacteria with natural chemotaxis towards methanol. We tried to enrich environmental chemotactic bacteria using a glass capillary that is half-filled with methanol solidified with agarose as a trap ("chemotaxis fishing"). The pilot experiment using methanol-chemotactic Methylobacterium aquaticum strain 22A enriched the cells by 46-fold. The method was then applied to bacterial suspensions from paddy water and plants. Depending on the isolation sources and the methods of motility induction, methylotrophic bacteria were enriched 1.2-330-fold. The fished isolates belong to ...
Source: Bioscience, Biotechnology, and Biochemistry - July 4, 2019 Category: Biochemistry Authors: Tola YH, Fujitani Y, Tani A Tags: Biosci Biotechnol Biochem Source Type: research

Suppression of LINC00707 alleviates lipopolysaccharide-induced inflammation and apoptosis in PC-12 cells by regulated miR-30a-5p/Neurod 1.
In this study, we found that LINC00707 expression was significantly up-regulated in lipopolysaccharide (LPS)-treated PC-12, a model that mimics nerve cell injury in an inflammatory environment after SCI. Suppression of LINC00707 alleviated LPS-induced inflammation and apoptosis in PC-12 cells. Furthermore, we found that LINC00707 adsorbed miR-30a-5p and silenced miR-30a-5p or overexpressed Neurod 1 reversed the effect of LINC00707 on the inflammation and apoptosis of LPS-treated PC-12 cells. These findings revealed that LINC00707 alleviates LPS-induced inflammation and apoptosis in PC-12 cells by targeting miR-30a-5p/Neuro...
Source: Bioscience, Biotechnology, and Biochemistry - July 4, 2019 Category: Biochemistry Authors: Zhu S, Zhou Z, Li Z, Shao J, Jiao G, Huang YE, Lin Y Tags: Biosci Biotechnol Biochem Source Type: research

Antioxidant activity of phenylethanoid glycosides on glutamate-induced neurotoxicity.
Abstract Exposure of PC12 cells to 10 mM glutamate caused significant viability loss, cell apoptosis, decreased activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as increased levels of malondialdehyde (MDA). In parallel, glutamate significantly increased the intracellular levels of ROS and intracellular calcium. However, pretreatment of the cells with acteoside and isoacteoside significantly suppressed glutamate-induced cellular events. Moreover, acteoside and isoacteoside reduced the glutamate-induced increase of caspase-3 activity and also ameliorated the glutamate-induced Bcl-...
Source: Bioscience, Biotechnology, and Biochemistry - July 4, 2019 Category: Biochemistry Authors: Ji SL, Cao KK, Zhao XX, Kang NX, Zhang Y, Xu QM, Yang SL, Liu YL, Wang C Tags: Biosci Biotechnol Biochem Source Type: research

Influence of continued ingestion of matcha on emotional behaviors after social stress in mice.
Abstract Matcha has high contents of theanine, caffeine, and epigallocatechin gallate (EGCG). Among these, theanine reduces psychological and physiological stress responses, although this effect is disturbed by caffeine and EGCG. It was reported that the continued ingestion of high-quality matcha, whose molar ratio of caffeine and EGCG to theanine and arginine was kept at less than two, reduces stress responses. However, most matcha on the market has an inadequate ratio. Therefore, we investigated the influence of continued ingestion of matcha with a ratio of over two, on emotional behaviors after stress. Continue...
Source: Bioscience, Biotechnology, and Biochemistry - July 3, 2019 Category: Biochemistry Authors: Monobe M, Nomura S, Ema K, Horie H Tags: Biosci Biotechnol Biochem Source Type: research

Biochemical characteristics of maltose phosphorylase MalE from Bacillus sp. AHU2001 and chemoenzymatic synthesis of oligosaccharides by the enzyme.
In this study, we characterized Bacillus sp. AHU2001 MP (MalE) that was produced in Escherichia coli. The enzyme exhibited phosphorolytic activity to maltose, but not to other α-linked glucobioses and maltotriose. The optimum pH and temperature of MalE for maltose-phosphorolysis were 8.1 and 45°C, respectively. MalE was stable at a pH range of 4.5-10.4 and at ≤40°C. The phosphorolysis of maltose by MalE obeyed the sequential Bi-Bi mechanism. In reverse phosphorolysis, MalE utilized d-glucose, 1,5-anhydro-d-glucitol, methyl α-d-glucoside, 2-deoxy-d-glucose, d-mannose, d-glucosamine, N-acetyl-d-glucosa...
Source: Bioscience, Biotechnology, and Biochemistry - July 2, 2019 Category: Biochemistry Authors: Gao Y, Saburi W, Taguchi Y, Mori H Tags: Biosci Biotechnol Biochem Source Type: research

Isolation and identification of N6-isopentenyladenosine as the cytotoxic constituent of a marine sponge Oceanapia sp.
Abstract N6-Isopentenyladenosine (i6A) was isolated from a marine sponge Oceanapia sp. as the major cytotoxic constituent along with N6-isopentenyladenosine 5'-monophosphate (i6AP) which was inactive. The structures of i6A and i6AP were assigned by a combination of the analysis of NMR spectroscopy and mass spectrometry. This is the first isolation of i6A and i6AP from a marine sponge. PMID: 31250707 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - June 28, 2019 Category: Biochemistry Authors: Nakamukai S, Ise Y, Ohtsuka S, Okada S, Matsunaga S Tags: Biosci Biotechnol Biochem Source Type: research

Flavone of Hippophae (H-flavone) lowers atherosclerotic risk factors via upregulation of the adipokine C1q/tumor necrosis factor-related protein 6 (CTRP6) in macrophages.
In this study, we examined the mechanism of Flavone of Hippophae (H-flavone) in regulating macrophage foaming and atherosclerosis (AS) plaque formation. H-flavone treatment increased the secretion of C1q/tumor necrosis factor-related proteins 6 (CTRP6) in Ox-LDL-treated mouse peripheral blood macrophage cells (PBMC) and significantly reduced the percentage of cholesteryl ester (CE) in PBMC. Additionally, H-flavone suppressed Ox-LDL-induced cell foaming and the production of inflammatory cytokines through upregulating CTPR6 expression. Next, we further validated the inhibitory effect of H-flavone on plaque formation and inf...
Source: Bioscience, Biotechnology, and Biochemistry - June 28, 2019 Category: Biochemistry Authors: Zhuo X, Tian Y, Wei Y, Deng Y, Wu Y, Chen T Tags: Biosci Biotechnol Biochem Source Type: research

Antioxidant enzyme activity and growth responses of Huangguogan citrus cultivar to nitrogen supplementation.
This study examined the physiological effects of different amounts of nitrogen (N) supplementation (0 to 2.72 kg/year) on the citrus cultivar Huangguogan (Citrus reticulata × Citrus sinensis). Root activity, chlorophyll content, and fruit quality were measured, and the activities of the antioxidant enzymes superoxide dismutase (SOD), guaiacol peroxidase (POD), and catalase (CAT), and the contents of malondialdehyde (MDA) and soluble protein in root, leaf, and fruit tissues were examined at different developmental stages. Root activity, chlorophyll content, fruit quality, antioxidant enzyme activity, MDA content, and ...
Source: Bioscience, Biotechnology, and Biochemistry - June 27, 2019 Category: Biochemistry Authors: Liao L, Dong T, Qiu X, Rong Y, Sun G, Wang Z, Zhu J Tags: Biosci Biotechnol Biochem Source Type: research

Extracts of bilberry (Vaccinium myrtillus L.) fruits improve liver steatosis and injury in mice by preventing lipid accumulation and cell death.
Abstract Bilberry has been reported to have anti-oxidant and anti-inflammatory properties. We studied the effect of bilberry (Vaccinium myrtillus L.) fruits extracts (BEs) on the pathogenesis caused by lipid accumulation in fatty liver and non-alcoholic steatohepatitis (NASH). 5 μg/ml of BEs was enough to suppress lipid accumulation in the fatty liver model of the mouse hepatic AML12 cells. BEs increased cell viability and anti-oxidant capacity, presumably by activating (phosphorylating) Akt/STAT3 and inducing MnSOD/catalase. BEs also significantly reduced Rubicon and induced p62/SQSTM1, possibly contributing t...
Source: Bioscience, Biotechnology, and Biochemistry - June 27, 2019 Category: Biochemistry Authors: Haga S, YiMin, Yamaki H, Jin S, Sogon T, Morita N, Ozaki M Tags: Biosci Biotechnol Biochem Source Type: research

Inhibitory effects of a sulfated polysaccharide isolated from edible red alga Bangia fusco-purpurea on α-amylase and α-glucosidase.
In this study, a sulfated polysaccharide (BFP) was isolated from the edible red alga Bangia fusco-purpurea. Gel-filtration and thin layer chromatographically analyses suggested that BFP was a homogenous polysaccharide. The chemical structural analysis revealed that BFP mainly consisted of galactose together with a small amount of uronic acid, mannose, and glucose. Its molecular mass was estimated to be 133.18 kDa by high-performance liquid chromatography (HPLC) analysis. BFP inhibited α-amylase and α-glucosidase in a concentration-dependent manner. The IC50 values of BFP against α-amylase and α-gluc...
Source: Bioscience, Biotechnology, and Biochemistry - June 27, 2019 Category: Biochemistry Authors: Jiang Z, Yu G, Liang Y, Song T, Zhu Y, Ni H, Yamaguchi K, Oda T Tags: Biosci Biotechnol Biochem Source Type: research

Production of recombinant extracellular cholesterol esterase using consistently active promoters in Burkholderia stabilis.
Abstract Burkholderia stabilis FERMP-21014 produces highly active cholesterol esterase in the presence of fatty acids. To develop an overexpression system for cholesterol esterase production, we carried out RNA sequencing analyses to screen strongly active promoters in FERMP-21014. Based on gene expression consistency analysis, we selected nine genes that were consistently expressed at high levels, following which we constructed expression vectors using their promoter sequences and achieved overproduction of extracellular cholesterol esterase under fatty acid-free conditions. Of the tested promoters, the promoter ...
Source: Bioscience, Biotechnology, and Biochemistry - June 19, 2019 Category: Biochemistry Authors: Yoshida K, Konishi K, Magana-Mora A, Rougny A, Yasutake Y, Muramatsu S, Murata S, Kumagai T, Aburatani S, Sakasegawa SI, Tamura T Tags: Biosci Biotechnol Biochem Source Type: research

Substrate recognition by bacterial solute-binding protein is responsible for import of extracellular hyaluronan and chondroitin sulfate from the animal host.
Abstract Glycosaminoglycans (GAGs) such as hyaluronan and chondroitin in animal extracellular matrices contain disaccharide-repeating units. In a Gram-negative pathogenic Streptobacillus moniliformis, which belongs to Fusobacteria phylum and resides in rodent oral cavities, the solute-binding protein (Smon0123)-dependent ATP-binding cassette transporter imports unsaturated hyaluronan/chondroitin disaccharides into the cytoplasm after GAG lyase-dependent depolymerization. Here we show substrate recognition of unsaturated hyaluronan disaccharide by Smon0123. Moreover, Smon0123 exhibited no affinity for unsaturated c...
Source: Bioscience, Biotechnology, and Biochemistry - June 16, 2019 Category: Biochemistry Authors: Oiki S, Sato M, Mikami B, Murata K, Hashimoto W Tags: Biosci Biotechnol Biochem Source Type: research

Oxytocin promotes heat stress tolerance via insulin signals in Caenorhabditis elegans.
Abstract Oxytocin, has various physiological functions that have been well studied and many that remain unknown. Here, we aimed to determine new physiological functions of oxytocin using Caenorhabditis elegans. Oxytocin treatment promoted the restoration of movement after heat stress and enhanced the viability under heat stress. However, oxytocin had no effect on the life span and only little effect on the oxidative stress tolerance. In contrast, oxytocin treatment didn't promote the restoration of movement or enhance the viability of deficient mutants of ntr-1/2, which is the gene encoding the oxytocin receptor. ...
Source: Bioscience, Biotechnology, and Biochemistry - June 14, 2019 Category: Biochemistry Authors: Yasuda K, Sakamoto K Tags: Biosci Biotechnol Biochem Source Type: research

Iso- α-acids and matured hop bitter acids in beer improve obesity-induced cognitive impairment.
Iso-α-acids and matured hop bitter acids in beer improve obesity-induced cognitive impairment. Biosci Biotechnol Biochem. 2019 Jun 14;:1-9 Authors: Ayabe T, Ohya R, Ano Y Abstract Dementia and cognitive decline have become public health issues worldwide and life-style-related diseases and obesity have recently been reported as key risk factors. We have recently demonstrated that short-term administration of iso-α-acids (IAAs), hop-derived bitter components of beer, improves spatial and object recognition memory. However, the short-term effects of IAAs on obesity-induced cognitive impairmen...
Source: Bioscience, Biotechnology, and Biochemistry - June 14, 2019 Category: Biochemistry Authors: Ayabe T, Ohya R, Ano Y Tags: Biosci Biotechnol Biochem Source Type: research

Metabolomic insights into polyhydroxyalkanoates production by halophilic bacteria with acetic acid as carbon source.
Abstract A metabolomics method was established to analyze changes of intracellular metabolites and study the mechanism for enhancing polyhydroxyalkanoates production by halotolerant bacteria, Bacillus cereus strain HY-3, using acetic acid as carbon source. Maximum poly(3-hydroxybutyrate) (PHB) contents for the medium with 0.5 g/L and 5.0 g/L of acetic acid were 41.0 ± 0.415% and 49.2 ± 1.21%. Principal components analysis revealed clear metabolic differences in different growth stages and different concentrations of carbon source. According to statistical analysis, 3-hydroxybutyrate (3-HB), serine, t...
Source: Bioscience, Biotechnology, and Biochemistry - June 14, 2019 Category: Biochemistry Authors: Wang P, Qiu YQ, Chen XT, Liang XF, Ren LH Tags: Biosci Biotechnol Biochem Source Type: research

Purification, characterization, and gene cloning of a novel aminoacylase from Burkholderia sp. strain LP5_18B that efficiently catalyzes the synthesis of N-lauroyl-l-amino acids.
Abstract An N-lauroyl-l-phenylalanine-producing bacterium, identified as Burkholderia sp. strain LP5_18B, was isolated from a soil sample. The enzyme was purified from the cell-free extract of the strain and shown to catalyze degradation and synthesis activities toward various N-acyl-amino acids. N-lauroyl-l-phenylalanine and N-lauroyl-l-arginine were obtained with especially high yields (51% and 89%, respectively) from lauric acid and l-phenylalanine or l-arginine by the purified enzyme in an aqueous system. The gene encoding the novel aminoacylase was cloned from Burkholderia sp. strain LP5_18B and expressed in ...
Source: Bioscience, Biotechnology, and Biochemistry - June 14, 2019 Category: Biochemistry Authors: Takakura Y, Asano Y Tags: Biosci Biotechnol Biochem Source Type: research

Genome editing to generate nonfoam-forming sake yeast strains.
Abstract Mutations frequently occur during breeding of sake yeasts and result in unexpected phenotypes. Here, genome editing tools were applied to develop an ideal nonfoam-forming sake yeast strain, K7GE01, which had homozygous awa1∆/awa1∆ deletion alleles that were responsible for nonfoam formation and few off-target mutations. High-dimensional morphological phenotyping revealed no detectable morphological differences between the genome-edited strain and its parent, while the canonical nonfoam-forming strain, K701, showed obvious morphological changes. Small-scale fermentation tests also showed differences be...
Source: Bioscience, Biotechnology, and Biochemistry - June 12, 2019 Category: Biochemistry Authors: Ohnuki S, Kashima M, Yamada T, Ghanegolmohammadi F, Zhou Y, Goshima T, Maruyama JI, Kitamoto K, Hirata D, Akao T, Ohya Y Tags: Biosci Biotechnol Biochem Source Type: research

Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose.
Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose. Biosci Biotechnol Biochem. 2019 Jun 12;:1-8 Authors: Kobayashi K, Nakajima M, Aramasa H, Kimura S, Iwata T, Nakai H, Taguchi H Abstract A large amount of β-1,2-glucan was produced enzymatically from quite a small amount of sophorose as an acceptor material through three synthesis steps using a sucrose phosphorylase and a 1,2-β-oligoglucan phosphorylase. The first synthesis step was performed in a 200 μL of a reaction solution containing 5 mM sophorose and 1.0 M sucrose. β-1,2-Glucan in a part o...
Source: Bioscience, Biotechnology, and Biochemistry - June 12, 2019 Category: Biochemistry Authors: Kobayashi K, Nakajima M, Aramasa H, Kimura S, Iwata T, Nakai H, Taguchi H Tags: Biosci Biotechnol Biochem Source Type: research

Cantharidin decreased viable cell number in human osteosarcoma U-2 OS cells through G2/M phase arrest and induction of cell apoptosis.
Abstract Cantharidin (CTD), a sesquiterpenoid bioactive substance, has been reported to exhibit anticancer activity against various types of cancer cells. The aim of the present study was to investigate the apoptosis effects and the underlying mechanisms of CTD on osteosarcoma U-2 OS cells. Results showed that CTD induced cell morphologic changes, reduced total viable cells, induced DNA damage, and G2/M phase arrest. CTD increased the production of reactive oxygen species and Ca2+, and elevated the activities of caspase-3 and -9, but decreased the level of mitochondrial membrane potential. Furthermore, CTD in...
Source: Bioscience, Biotechnology, and Biochemistry - June 12, 2019 Category: Biochemistry Authors: Chen CC, Chueh FS, Peng SF, Huang WW, Tsai CH, Tsai FJ, Huang CY, Tang CH, Yang JS, Hsu YM, Yin MC, Huang YP, Chung JG Tags: Biosci Biotechnol Biochem Source Type: research

Feeding Aspergillus protease preparation combined with adequate protein diet to rats increases levels of cecum gut-protective amino acids, partially linked to Bifidobacterium and Lactobacillus.
In this study, dietary AP markedly increased the relative abundance of Bifidobacterium and Lactobacillus and the levels of free threonine, alanine, proline, taurine, ornithine, phenylalanine, cystine, and γ-aminobutyric acid in the cecum contents of rats fed with an adequate protein diet, but not in those fed with a low-protein diet. The elevated AAs, except ornithine and phenylalanine, potentially have gut-related health benefits. Some of the AP-modulated free AAs appeared to be associated with the relative abundance of Bifidobacterium and Lactobacillus. Thus, AP combined with adequate protein diet is likely to incr...
Source: Bioscience, Biotechnology, and Biochemistry - June 11, 2019 Category: Biochemistry Authors: Yang Y, Kumrungsee T, Kuroda M, Yamaguchi S, Kato N Tags: Biosci Biotechnol Biochem Source Type: research

Characterization of AtWRI1 in fatty acids and starch synthesis in rice.
Abstract WRINKLED1 (WRI1) belongs to AP2/EREBP transcription factor. Its function in dicots for fatty acids synthesis has been deeply studied, but its role in monocot, especially in rice, is still poorly understood. Here, with the overexpression of AtWRI1 in rice, we found its overexpression increased fatty acids content in vegetative organs and seed coat including aleurone layer (SCAL) but decreased fatty acids content in endosperm. Meanwhile, the overexpression of AtWRI1 increased starch content in endosperm. These results provide a new insight into the function of AtWRI1in monocot and make a previous basement f...
Source: Bioscience, Biotechnology, and Biochemistry - June 9, 2019 Category: Biochemistry Authors: Yang J, Tian R, Gao Z, Yang H Tags: Biosci Biotechnol Biochem Source Type: research

Adenosine A2A receptor deficiency prevents p38MAPK activation and apoptosis of mouse hippocampal cells in the chronic hypoxic-hypercapnia model.
This study aims to study the effects of adenosine A2A receptor (A2AR) on hippocampal cell apoptosis and the putative mechanisms in a mouse model of chronic hypoxic-hypercapnia. Wild-type (WT) or A2AR knockout (A2AR KO) mice were randomly divided into normal control (NC) groups and chronic hypoxic-hypercapnia (4HH) groups. Compared with their corresponding NC groups (WT-NC and KO-NC), the apoptosis index (AI), caspase-3 activity, Bax mRNA and P-p38 protein expression in the hippocampus of 4HH groups (WT-4HH and KO-4HH) were significantly increased, while Bcl2 mRNA expression was significantly decreased (P 
Source: Bioscience, Biotechnology, and Biochemistry - June 9, 2019 Category: Biochemistry Authors: Ren H, Guo X, Wang X, Cui Z Tags: Biosci Biotechnol Biochem Source Type: research

Ubr1p-Cup9p-Ptr2p pathway involves in the sensitivity to readthrough compounds negamycin derivatives in budding yeast.
In this study, we found that dipeptide transporter Ptr2p is the putative transporter of read-through compounds (+)-negamycin derivatives TCP-126 and TCP-112, in budding yeast. Ptr2p expression and activity were correlated with the TCP-112 sensitivity, and dipeptide with high affinity to Ptr2p suppressed the TCP-112 activity. These results suggest that dipeptide transporter is one of the determinants of negamycin analogs sensitivity. Abbreviation: PTC: premature termination codon. PMID: 31159660 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - June 4, 2019 Category: Biochemistry Authors: Hamada K, Naito A, Hamaguchi Y, Kanesaki Y, Kasahara K, Taguchi A, Omura N, Hayashi Y, Usui T Tags: Biosci Biotechnol Biochem Source Type: research

Regulatory mechanisms for amylolytic gene expression in the koji mold Aspergillus oryzae.
Abstract The koji mold Aspergillus oryzae has been used in traditional Japanese food and beverage fermentation for over a thousand years. Amylolytic enzymes are important in sake fermentation, wherein production is induced by starch or malto-oligosaccharides. This inducible production requires at least two transcription activators, AmyR and MalR. Among amylolytic enzymes, glucoamylase GlaB is produced exclusively in solid-state culture and plays a critical role in sake fermentation owing to its contribution to glucose generation from starch. A recent study demonstrated that glaB gene expression is regulated by a n...
Source: Bioscience, Biotechnology, and Biochemistry - June 4, 2019 Category: Biochemistry Authors: Gomi K Tags: Biosci Biotechnol Biochem Source Type: research

A new in vitro muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration.
Abstract Several food constituents augment exercise-induced muscle strength improvement; however, the detailed mechanism underlying these combined effects is unknown because of the lack of a cultured cell model for evaluating the contraction-induced muscle protein synthesis level. Here, we aimed to establish a new in vitro muscle contraction model for analyzing the activation of mammalian target of rapamycin complex 1 (mTORC1) signaling. We adopted the tetanic electric stimulation of 50 V at 100 Hz for 10 min in L6.C11 myotubes. Akt, ERK1/2, and p70S6K phosphorylation increased significantly after electrical pulse...
Source: Bioscience, Biotechnology, and Biochemistry - June 4, 2019 Category: Biochemistry Authors: Sato S, Nomura M, Yamana I, Uchiyama A, Furuichi Y, Manabe Y, Fujii NL Tags: Biosci Biotechnol Biochem Source Type: research

Development of a synthesis method for odor sesquiterpenoid, (-)-rotundone, using non-heme Fe2+-chelate catalyst and ferric-chelate reductase.
Abstract (-)-Rotundone, a sesquiterpenoid that has a characteristic woody and peppery odor, is a key aroma component of spicy foodstuffs, such as black pepper and Australian Shiraz wine. (-)-Rotundone shows the lowest level of odor threshold in natural compounds and remarkably improves the quality of various fruit flavors. To develop a method for the synthesis of (-)-rotundone, we focused on non-heme Fe2+-chelates, which are biomimetic catalysts of the active center of oxygenases and enzymatic supply and regeneration of those catalysts. That is, we constructed a unique combination system composed of the oxidative ...
Source: Bioscience, Biotechnology, and Biochemistry - June 4, 2019 Category: Biochemistry Authors: Umezawa S, Konishi S, Kino K Tags: Biosci Biotechnol Biochem Source Type: research

Purification and enzymatic characterization of trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from Rhodococcus opacus and enzymatic formation of α, β-unsaturated ketones.
Purification and enzymatic characterization of trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from Rhodococcus opacus and enzymatic formation of α, β-unsaturated ketones. Biosci Biotechnol Biochem. 2019 Jun 04;:1-5 Authors: Suzuki T, Takizawa N Abstract Trans-o-hydroxybenzylidenepyruvate (tHBPA) hydratase-aldolase (RnoE) catalyzes the conversion of tHBPA to 2-hydroxybenzaldehyde and pyruvate. We purified RnoE from Rhodococcus opacus and characterized its enzymatic properties. It exhibited maximum enzyme activity at 60°C and catalyzed the reverse reaction, converting various aroma...
Source: Bioscience, Biotechnology, and Biochemistry - June 4, 2019 Category: Biochemistry Authors: Suzuki T, Takizawa N Tags: Biosci Biotechnol Biochem Source Type: research

In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6.
Abstract TRAF6 is highly expressed in many tumors and plays an important role in the immune system. The aim of this study is to confirm anti-tumor activities of all naturally occurring Cinchona alkaloids that have been screened using computational docking program, and to validate the accuracy and specificity of the RING domain of TRAF6 as a potential anti-tumor target, and to explore their effect on the immune system. Results reported herein would demonstrate that Cinchona alkaloids could induce apoptosis in HeLa cells, inhibit the ubiquitination and phosphorylation of both AKT and TAK1, and up-regulate the ratio ...
Source: Bioscience, Biotechnology, and Biochemistry - May 11, 2019 Category: Biochemistry Authors: Qi Y, Zhao X, Chen J, Pradipta AR, Wei J, Ruan H, Zhou L, Hsung RP, Tanaka K Tags: Biosci Biotechnol Biochem Source Type: research

Human TRPA1 activation by terpenes derived from the essential oil of daidai, Citrus aurantium L. var. daidai Makino.
Abstract Daidai (bitter orange, Citrus aurantium) is characterized by its fresh citrus scent. In Japanese cuisine, its juice is an important ingredient. As tons of industrial waste is obtained while processing the daidai juice, additional utilization of this waste has great social value. In our study, we prepared the essential oil from the waste obtained during daidai juice processing and demonstrated that the oil activates human TRPA1 (hTRPA1). This oil contains 10 types of terpenes, all of which activated hTRPA1 with an EC50 value of 6-167 µM. To our knowledge, this study is the first to show a hTRPA1...
Source: Bioscience, Biotechnology, and Biochemistry - May 10, 2019 Category: Biochemistry Authors: Terada Y, Yamashita R, Ihara N, Yamazaki-Ito T, Takahashi Y, Masuda H, Sakuragawa S, Ito S, Ito K, Watanabe T Tags: Biosci Biotechnol Biochem Source Type: research

G-quadruplex-forming GGA repeat region functions as a negative regulator of the Ccnb1ip1 enhancer.
Abstract An enhancer located upstream of the transcriptional start site of Ccnb1ip1 containing two GGA-rich regions and a 14-GGA repeat (GGA)14 region has been previously identified. Three copies of four GGA repeats in the c-myb promoter that form a tetrad:heptad:heptad:tetrad (T:H:H:T) dimerized G-quadruplex (G4) structure reportedly functions as both a transcriptional repressor and activator. Here, the secondary structures of the two GGA-rich and (GGA)14 regions were analyzed using circular dichroism spectral analysis, which indicated that the two GGA-rich DNAs formed parallel-type G4 structures, whereas (GGA)14...
Source: Bioscience, Biotechnology, and Biochemistry - May 7, 2019 Category: Biochemistry Authors: Islam I, Baba Y, Witarto AB, Yoshida W Tags: Biosci Biotechnol Biochem Source Type: research

Maltobionic acid enhances intestinal absorption of calcium and magnesium in rats.
Abstract In Experiment 1, the effects of calcium maltobionate (MBCa) on calcium and magnesium absorption were examined using male rats. Four diets were designed in which 25%, 50%, and 100% of calcium carbonate (CaCO3, Control) were substituted with MBCa and were designated as MBCa-25, MBCa-50, and MBCa-100, respectively. The cecal concentration of short-chain fatty acids was significantly higher in groups MBCa-50 and MBCa-100; however, pH of cecal contents did not significantly differ among the groups. Retention rates of calcium and magnesium were significantly higher in all MBCa groups as compared to the Control....
Source: Bioscience, Biotechnology, and Biochemistry - May 6, 2019 Category: Biochemistry Authors: Suehiro D, Okada M, Fukami K, Nakagawa T, Hayakawa T Tags: Biosci Biotechnol Biochem Source Type: research

Gene cloning of the pink-colored protein from Pleurotus salmoneostramineus (PsPCP) and its species-specific chromoprotein are effective for colorization of the fruit body.
Abstract Pleurotus salmoneostramineus is a pink mushroom. This pink color is a protein and forms a complex with 3H-indol-3-one. The gene encoding the pink-colored protein from P. salmoneostramineus (PsPCP) was cloned, and its sequence was elucidated as a 681-bp. The ORF encodes 226 amino acid residues. The amino acid sequence of the protein did not show any significant homology in the DDBJ/EMBL/GenBank databases. Recombinant PsPCP was expressed as the soluble form in E. coli. The reaction mixture of purified recombinant PsPCP and 3H-indol-3-one showed a pink color as the native pigment. A real-time PCR analysis re...
Source: Bioscience, Biotechnology, and Biochemistry - May 6, 2019 Category: Biochemistry Authors: Fukuta Y, Kamei K, Matsui A, Fuji Y, Onuma H, Shirasaka N Tags: Biosci Biotechnol Biochem Source Type: research

An easy, inexpensive, and sensitive method for the quantification of chitin in insect peritrophic membrane by image processing.
In this study, we establish a facile method for the quantification of chitin in peritrophic membrane by image processing. In this method, chitin was indirectly quantified using chitosan-I3- complex, which exhibited a specific red-purple color. A calibration curve using a chitosan solution showed good linearity in a concentration range of 0.05-0.5 μg/μL. We quantified the amount of chitin in peritrophic membrane of Spodoptera litura (Lepidoptera: Noctuidae) larvae using this method. Throughout the study, only common inexpensive regents and easily attainable apparatuses were employed. This method can be easily app...
Source: Bioscience, Biotechnology, and Biochemistry - May 3, 2019 Category: Biochemistry Authors: Nakata R, Yoshinaga N, Teraishi M, Okumoto Y, Mori N Tags: Biosci Biotechnol Biochem Source Type: research

Soy hydrolysate enhances the isoproterenol-stimulated lipolytic pathway through an increase in β-adrenergic receptor expression in adipocytes.
Soy hydrolysate enhances the isoproterenol-stimulated lipolytic pathway through an increase in β-adrenergic receptor expression in adipocytes. Biosci Biotechnol Biochem. 2019 May 02;:1-8 Authors: Ng SP, Nomura W, Mohri S, Takahashi H, Jheng HF, Ara T, Nagai H, Ito T, Kawada T, Goto T Abstract Activation of the adipose lipolytic pathway during lipid metabolism is mediated by protein kinase A (PKA), which responds to β-adrenergic stimulation, leading to increased lipolysis. Soy is well known as a functional food and it is able to affect lipolysis in adipocytes. However, the mechanism by which ...
Source: Bioscience, Biotechnology, and Biochemistry - May 2, 2019 Category: Biochemistry Authors: Ng SP, Nomura W, Mohri S, Takahashi H, Jheng HF, Ara T, Nagai H, Ito T, Kawada T, Goto T Tags: Biosci Biotechnol Biochem Source Type: research

A novel Ca2+-signal transduction inhibitor, kujigamberol C, isolated from Kuji amber.
Abstract A novel labdane type diterpenoid, 15-nor-8-labden-13-ol, named kujigamberol C, was isolated from Kuji amber using a modified isolation method to increase the yield of biologically active compounds. The structure was determined using HREIMS, 1D and 2D NMR. Kujigamberol C showed growth-restoring activity against mutant yeast via Ca2+-signal transduction. PMID: 31045479 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - May 2, 2019 Category: Biochemistry Authors: Takahashi H, Koshino H, Maruyama M, Shinden H, Kimura KI Tags: Biosci Biotechnol Biochem Source Type: research

Generation of temperature sensitive mutations with error-prone PCR in agene encoding acomponent of the spindle pole body in fission yeast.
We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to pcp1, and can be suited to any essential genes involved in other processes. PMID: 31042107 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - May 1, 2019 Category: Biochemistry Authors: Tang NH, Fong CS, Masuda H, Jourdain I, Yukawa M, Toda T Tags: Biosci Biotechnol Biochem Source Type: research

Exploratory investigation of the anti-inflammatory effects of RNase A-treated Enterococcus faecalis strain EC-12.
This study aimed to investigate if RNase A-treated EC-12 could also produce IL-10 and to evaluate the possible effects of IL-10 produced by RNase A-treated EC-12. Three experiments were conducted: (1) Assessment of the effect of RNase A-treated EC-12 on transcriptome profiles and biological pathways in human peripheral blood mononuclear cells; (2) Determination of cytokine concentration in its culture supernatants; and (3) Supplementation of RNase A-treated EC-12 (RN) to mice with dextran sodium sulfate-induced colitis. Treatment of EC-12 with RNase A inhibited inflammatory response including the potency to induce IL-12 pr...
Source: Bioscience, Biotechnology, and Biochemistry - April 30, 2019 Category: Biochemistry Authors: Tamaki R, Takahashi M, Tai S, Makioka-Itaya Y, Ijich T, Inoue R Tags: Biosci Biotechnol Biochem Source Type: research

Tumor-suppressive activity of Hnf1 β in Wilms' tumor.
Tumor-suppressive activity of Hnf1β in Wilms' tumor. Biosci Biotechnol Biochem. 2019 Apr 30;:1-8 Authors: Liu Y, Kanyomse Q, Xie Y Abstract Hepatocyte nuclear factor 1β (HNF1β) is a transcription factor belonging to the HNF-1 family and has been implicated in a number of cancers, but its role in Wilms' tumor (nephroblastoma) has not been addressed. Here, we compared its expression between Wilms' tumor patient kidney tissue and adjacent tissue based on the Oncomine database ( www.oncomine.com ). Cell proliferation, apoptosis, migration, and HNF1β expression level were analyzed in Wi...
Source: Bioscience, Biotechnology, and Biochemistry - April 30, 2019 Category: Biochemistry Authors: Liu Y, Kanyomse Q, Xie Y Tags: Biosci Biotechnol Biochem Source Type: research

Peppermint (Mentha piperita L.) extract effectively inhibits cytochrome P450 3A4 (CYP3A4) mRNA induction in rifampicin-treated HepG2 cells.
Abstract Interaction between foods and drugs is an important consideration in pharmaceutical therapy. Therefore, here, we examined the suppressive effects of the extracts from seven edible herbs on the induction of CYP3A4 gene expression in rifampicin-treated HepG2 cells. We evaluated the structure and suppressive activity of the most effective active compound isolated from dried peppermint (Mentha piperita L.). The structure of the compound was identified as that of pheophorbide a based on spectroscopic data. It suppressed the induction of CYP3A4 mRNA expression by rifampicin in a dose-dependent manner. Quantitat...
Source: Bioscience, Biotechnology, and Biochemistry - April 27, 2019 Category: Biochemistry Authors: Kobayashi T, Sugaya K, Onose JI, Abe N Tags: Biosci Biotechnol Biochem Source Type: research

Pyrophosphate amplification reaction for measuring amino acid concentrations with high sensitivity using aminoacyl-tRNA synthetase from Escherichia coli.
Abstract To measure amino acid concentrations with high sensitivity, the pyrophosphate amplification reaction conditions of histidyl-tRNA synthetase (HisRS) and tyrosyl-tRNA synthetase (TyrRS) were examined. The amount of pyrophosphate produced by reactions involving HisRS and TyrRS was amplified compared with the amount of the initial substrate L-amino acid after the addition of excess adenosine-5'-triphosphate and magnesium ions, with incubation at 50°C in an alkaline pH. The amount of pyrophosphate produced in the HisRS and TyrRS reactions was approximately 24- and 16-fold higher than the initial amount of ...
Source: Bioscience, Biotechnology, and Biochemistry - April 27, 2019 Category: Biochemistry Authors: Nakatsuka T, Aoki H, Kida M, Kugimiya A Tags: Biosci Biotechnol Biochem Source Type: research

Production of the plant polyketide curcumin in Aspergillus oryzae: strengthening malonyl-CoA supply for yield improvement.
This study provided useful information for heterologous polyketide production in A. oryzae. PMID: 31023165 [PubMed - as supplied by publisher] (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - April 26, 2019 Category: Biochemistry Authors: Kan E, Katsuyama Y, Maruyama JI, Tamano K, Koyama Y, Ohnishi Y Tags: Biosci Biotechnol Biochem Source Type: research

Bacterial communication through membrane vesicles.
Abstract Bacteria can communicate through diffusible signaling molecules that are perceived by cognate receptors. It is now well established that bacterial communication regulates hundreds of genes. Hydrophobic molecules which do not diffuse in aqueous environments alone have been identified in bacterial communication, that raised the question on how these molecules are transported between cells and trigger gene expressions. Recent studies show that these hydrophobic signaling molecules, including a long-chain N-acyl homoserine lactone signal produced in Paracoccus denitrificans, are carried by membrane vesicles (...
Source: Bioscience, Biotechnology, and Biochemistry - April 25, 2019 Category: Biochemistry Authors: Toyofuku M Tags: Biosci Biotechnol Biochem Source Type: research

Mechanism of neoagarotetraose protects against intense exercise-induced liver injury based on molecular ecological network analysis.
Abstract Here we have explored the effect of neoagarotetraose (NAT) on liver injury caused by intense exercise. Our results showed that NAT treatment obviously decreased liver weight (p
Source: Bioscience, Biotechnology, and Biochemistry - April 25, 2019 Category: Biochemistry Authors: Chen X, Yu J, Xue C, Wang Y, Tang Q, Mao X Tags: Biosci Biotechnol Biochem Source Type: research