Streamlining Considerations for Safety Measures: A Predictive Model for Addition of Clinically Significant Adverse Reactions to Japanese Drug Package Inserts
In conclusion, the proposed model effectively predicted CSAR additions to PIs resulting from the accumulation of ADR cases using data from both Japan and the United States.PMID:38479885 | DOI:10.1248/bpb.b23-00846 (Source: Biological and Pharmaceutical Bulletin)
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Takashi Watanabe Kaori Ambe Masahiro Tohkin Source Type: research

Structural Impact Assessment of Cytochrome P450 2A13 Polymorphisms Using Molecular Dynamics Simulations
This study aimed to unravel the mechanism underlying the diminished enzymatic activity of CYP2A13 variants by investigating their three-dimensional structures through molecular dynamics (MD) simulations. For each variant, MD simulations of 1000 ns were performed, and the obtained results were compared with those of the wild type. The findings indicated alterations in the interaction with heme in CYP2A13.4, .6, .8, and .9. In the case of CYP2A13.5, observable effects on the helix structure related to the interaction with the redox partner were identified. These conformational changes were sufficient to cause a decrease in e...
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Koichi Kato Tomoki Nakayoshi Sho Hioki Masahiro Hiratsuka Yoshinobu Ishikawa Eiji Kurimoto Akifumi Oda Source Type: research

Streamlining Considerations for Safety Measures: A Predictive Model for Addition of Clinically Significant Adverse Reactions to Japanese Drug Package Inserts
In conclusion, the proposed model effectively predicted CSAR additions to PIs resulting from the accumulation of ADR cases using data from both Japan and the United States.PMID:38479885 | DOI:10.1248/bpb.b23-00846 (Source: Biological and Pharmaceutical Bulletin)
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Takashi Watanabe Kaori Ambe Masahiro Tohkin Source Type: research

Structural Impact Assessment of Cytochrome P450 2A13 Polymorphisms Using Molecular Dynamics Simulations
This study aimed to unravel the mechanism underlying the diminished enzymatic activity of CYP2A13 variants by investigating their three-dimensional structures through molecular dynamics (MD) simulations. For each variant, MD simulations of 1000 ns were performed, and the obtained results were compared with those of the wild type. The findings indicated alterations in the interaction with heme in CYP2A13.4, .6, .8, and .9. In the case of CYP2A13.5, observable effects on the helix structure related to the interaction with the redox partner were identified. These conformational changes were sufficient to cause a decrease in e...
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Koichi Kato Tomoki Nakayoshi Sho Hioki Masahiro Hiratsuka Yoshinobu Ishikawa Eiji Kurimoto Akifumi Oda Source Type: research

Streamlining Considerations for Safety Measures: A Predictive Model for Addition of Clinically Significant Adverse Reactions to Japanese Drug Package Inserts
In conclusion, the proposed model effectively predicted CSAR additions to PIs resulting from the accumulation of ADR cases using data from both Japan and the United States.PMID:38479885 | DOI:10.1248/bpb.b23-00846 (Source: Biological and Pharmaceutical Bulletin)
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Takashi Watanabe Kaori Ambe Masahiro Tohkin Source Type: research

Structural Impact Assessment of Cytochrome P450 2A13 Polymorphisms Using Molecular Dynamics Simulations
This study aimed to unravel the mechanism underlying the diminished enzymatic activity of CYP2A13 variants by investigating their three-dimensional structures through molecular dynamics (MD) simulations. For each variant, MD simulations of 1000 ns were performed, and the obtained results were compared with those of the wild type. The findings indicated alterations in the interaction with heme in CYP2A13.4, .6, .8, and .9. In the case of CYP2A13.5, observable effects on the helix structure related to the interaction with the redox partner were identified. These conformational changes were sufficient to cause a decrease in e...
Source: Biological and Pharmaceutical Bulletin - March 13, 2024 Category: Drugs & Pharmacology Authors: Koichi Kato Tomoki Nakayoshi Sho Hioki Masahiro Hiratsuka Yoshinobu Ishikawa Eiji Kurimoto Akifumi Oda Source Type: research

Causes of Sex Differences in Serum Cholesterol and Triglyceride Levels in Meishan Pigs
Biol Pharm Bull. 2024;47(3):606-610. doi: 10.1248/bpb.b23-00895.ABSTRACTTo clarify the causes of sex differences (male < female) in the serum total cholesterol (TCHO) and triglyceride (TG) levels in Meishan pigs, we examined the sex differences in mRNA levels of key hepatic enzymes involved in the biosynthesis/metabolism of cholesterol and TG using real-time RT-PCR. There were no sex differences in mRNA levels of 3-hydroxy-3-methylglutaryl-CoA reductase and CYP51A1 for cholesterol biosynthesis, or of the rate-limiting enzyme CYP7A1 for bile acid synthesis from cholesterol. By contrast, sex differences (male < female)...
Source: Biological and Pharmaceutical Bulletin - March 10, 2024 Category: Drugs & Pharmacology Authors: Misaki Kojima Masakuni Degawa Source Type: research

Causes of Sex Differences in Serum Cholesterol and Triglyceride Levels in Meishan Pigs
Biol Pharm Bull. 2024;47(3):606-610. doi: 10.1248/bpb.b23-00895.ABSTRACTTo clarify the causes of sex differences (male < female) in the serum total cholesterol (TCHO) and triglyceride (TG) levels in Meishan pigs, we examined the sex differences in mRNA levels of key hepatic enzymes involved in the biosynthesis/metabolism of cholesterol and TG using real-time RT-PCR. There were no sex differences in mRNA levels of 3-hydroxy-3-methylglutaryl-CoA reductase and CYP51A1 for cholesterol biosynthesis, or of the rate-limiting enzyme CYP7A1 for bile acid synthesis from cholesterol. By contrast, sex differences (male < female)...
Source: Biological and Pharmaceutical Bulletin - March 10, 2024 Category: Drugs & Pharmacology Authors: Misaki Kojima Masakuni Degawa Source Type: research

Causes of Sex Differences in Serum Cholesterol and Triglyceride Levels in Meishan Pigs
Biol Pharm Bull. 2024;47(3):606-610. doi: 10.1248/bpb.b23-00895.ABSTRACTTo clarify the causes of sex differences (male < female) in the serum total cholesterol (TCHO) and triglyceride (TG) levels in Meishan pigs, we examined the sex differences in mRNA levels of key hepatic enzymes involved in the biosynthesis/metabolism of cholesterol and TG using real-time RT-PCR. There were no sex differences in mRNA levels of 3-hydroxy-3-methylglutaryl-CoA reductase and CYP51A1 for cholesterol biosynthesis, or of the rate-limiting enzyme CYP7A1 for bile acid synthesis from cholesterol. By contrast, sex differences (male < female)...
Source: Biological and Pharmaceutical Bulletin - March 10, 2024 Category: Drugs & Pharmacology Authors: Misaki Kojima Masakuni Degawa Source Type: research

Combined Experiments with in Vivo Fiber Photometry and Behavior Tests Can Facilitate the Measurement of Neuronal Activity in the Primary Somatosensory Cortex and Hyperalgesia in an Inflammatory Pain Mice Model
In this study, to examine whether the interaction between change of neuronal activity in S1 and hyperalgesia in hind paw before and after causing inflammatory pain was detected from same animal, the combined experiment of in vivo fiber photometry system and von Frey hairs test was applied. This combined experiment detected that amplitude of calcium responses in S1 neurons increased and the mechanical threshold of hind paw decreased from same animals which have an inflammatory pain. Moreover, we found that the values between amplitude of calcium responses and mechanical thresholds were shifted to negative correlation after ...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Tatsuya Ishikawa Daisuke Uta Hiroaki Okuda Ilia Potapenko Kiyomi Hori Toshiaki Kume Noriyuki Ozaki Source Type: research

Temperature-Dependent Upregulation of Per2 Protein Expression Is Mediated by eIF2 α Kinases PERK and PKR through PI3K Activation
Biol Pharm Bull. 2024;47(3):600-605. doi: 10.1248/bpb.b23-00739.ABSTRACTTemperature-dependent translational control of the core clock gene Per2 plays an important role in establishing entrainment of the circadian clock to physiological body temperature cycles. Previously, we found an involvement of the phosphatidylinositol 3-kinase (PI3K) in causing Per2 protein expression in response to a warm temperature shift (WTS) within a physiological range (from 35 to 38.5 °C). However, signaling pathway mediating the Per2 protein expression in response to WTS is only sparsely understood. Additional factor(s) other than PI3K remain...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Xinyan Shao Takahito Miyake Yuichi Inoue Emi Hasegawa Masao Doi Source Type: research

Combined Experiments with in Vivo Fiber Photometry and Behavior Tests Can Facilitate the Measurement of Neuronal Activity in the Primary Somatosensory Cortex and Hyperalgesia in an Inflammatory Pain Mice Model
In this study, to examine whether the interaction between change of neuronal activity in S1 and hyperalgesia in hind paw before and after causing inflammatory pain was detected from same animal, the combined experiment of in vivo fiber photometry system and von Frey hairs test was applied. This combined experiment detected that amplitude of calcium responses in S1 neurons increased and the mechanical threshold of hind paw decreased from same animals which have an inflammatory pain. Moreover, we found that the values between amplitude of calcium responses and mechanical thresholds were shifted to negative correlation after ...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Tatsuya Ishikawa Daisuke Uta Hiroaki Okuda Ilia Potapenko Kiyomi Hori Toshiaki Kume Noriyuki Ozaki Source Type: research

Temperature-Dependent Upregulation of Per2 Protein Expression Is Mediated by eIF2 α Kinases PERK and PKR through PI3K Activation
Biol Pharm Bull. 2024;47(3):600-605. doi: 10.1248/bpb.b23-00739.ABSTRACTTemperature-dependent translational control of the core clock gene Per2 plays an important role in establishing entrainment of the circadian clock to physiological body temperature cycles. Previously, we found an involvement of the phosphatidylinositol 3-kinase (PI3K) in causing Per2 protein expression in response to a warm temperature shift (WTS) within a physiological range (from 35 to 38.5 °C). However, signaling pathway mediating the Per2 protein expression in response to WTS is only sparsely understood. Additional factor(s) other than PI3K remain...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Xinyan Shao Takahito Miyake Yuichi Inoue Emi Hasegawa Masao Doi Source Type: research

Combined Experiments with in Vivo Fiber Photometry and Behavior Tests Can Facilitate the Measurement of Neuronal Activity in the Primary Somatosensory Cortex and Hyperalgesia in an Inflammatory Pain Mice Model
In this study, to examine whether the interaction between change of neuronal activity in S1 and hyperalgesia in hind paw before and after causing inflammatory pain was detected from same animal, the combined experiment of in vivo fiber photometry system and von Frey hairs test was applied. This combined experiment detected that amplitude of calcium responses in S1 neurons increased and the mechanical threshold of hind paw decreased from same animals which have an inflammatory pain. Moreover, we found that the values between amplitude of calcium responses and mechanical thresholds were shifted to negative correlation after ...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Tatsuya Ishikawa Daisuke Uta Hiroaki Okuda Ilia Potapenko Kiyomi Hori Toshiaki Kume Noriyuki Ozaki Source Type: research

Temperature-Dependent Upregulation of Per2 Protein Expression Is Mediated by eIF2 α Kinases PERK and PKR through PI3K Activation
Biol Pharm Bull. 2024;47(3):600-605. doi: 10.1248/bpb.b23-00739.ABSTRACTTemperature-dependent translational control of the core clock gene Per2 plays an important role in establishing entrainment of the circadian clock to physiological body temperature cycles. Previously, we found an involvement of the phosphatidylinositol 3-kinase (PI3K) in causing Per2 protein expression in response to a warm temperature shift (WTS) within a physiological range (from 35 to 38.5 °C). However, signaling pathway mediating the Per2 protein expression in response to WTS is only sparsely understood. Additional factor(s) other than PI3K remain...
Source: Biological and Pharmaceutical Bulletin - March 6, 2024 Category: Drugs & Pharmacology Authors: Xinyan Shao Takahito Miyake Yuichi Inoue Emi Hasegawa Masao Doi Source Type: research