Ultrasonic nebulization extraction/low pressure photoionization mass spectrometry for direct analysis of chemicals in matrices.
Abstract
A novel ultrasonic nebulization extraction/low-pressure photoionization (UNE-LPPI) system has been designed and employed for the rapid mass spectrometric analysis of chemicals in matrices. An ultrasonic nebulizer was used to extract the chemicals in solid sample and nebulize the solvent in the nebulization cell. Aerosols formed by ultrasonic were evaporated by passing through a transferring tube, and desolvated chemicals were ionized by the emitted light (10.6 eV) from a Krypton discharge lamp at low pressure (∼68 Pa). First, a series of semi/non-volatile compounds with different polarities, s...
Source: Analytica Chimica Acta - September 3, 2015 Category: Chemistry Authors: Liu C, Zhu Y, Zhou Z, Yang J, Qi F, Pan Y Tags: Anal Chim Acta Source Type: research
The Antimicrobial and Antioxidant Effects of Citrus aurantium L. Flowers (Bahar Narang) Extract in Traditional Yoghurt Stew during Refrigerated Storage
Abstract
The antimicrobial and antioxidant effects of Citrus aurantium L. flowers (Bahar narang) extract (BE; 500, 1,000 and 2,000 ppm) were studied in traditional yoghurt stew during storage at 4C for 28 days. The contents of total phenolics and flavonoids in BE were 81 ± 1.8 and 46 ± 2.4 mg/g, respectively. In vitro antibacterial activity of BE showed that BE had a strong antimicrobial activity against Escherichia coli O157:H7, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus. Application of BE in traditional yoghurt stew decreased the growth rate of all tested pathogens and P. aeruginosa...
Source: Journal of Food Safety - August 16, 2015 Category: Food Science Authors: Seyed Mohammad Bagher Hashemi, Razieh Amininezhad, Elham Shirzadinezhad, Mina Farahani, Seyed Hossein Asadi Yousefabad Tags: Original Article Source Type: research
An untargeted metabolomics-driven approach based on LC–TOF/MS and LC–MS/MS for the screening of xenobiotics and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma
Publication date: 10 November 2015 Source:Journal of Pharmaceutical and Biomedical Analysis, Volume 115 Author(s): Huan Wu, Xixi Li, Xuemei Yan, Li An, Kaiwen Luo, Mingjing Shao, Yue Jiang, Rui Xie, Fang Feng Zhi-Zi-Da-Huang decoction (ZZDHD), a typical traditional Chinese medicine prescription, is widely used in clinical practice for the treatment of alcoholic liver disease. However, due to lack of holistic metabolic research, the active ingredients of ZZDHD have not been fully elucidated. It entails a huge obstacle for the quality evaluation, pharmacokinetic studies and clinical-safe medication adm...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2015 Category: Drugs & Pharmacology Source Type: research
High-performance thin-layer chromatography/desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus aurantium L. (Rutaceae).
CONCLUSIONS: HPTLC/DESI-MS imaging is a relatively fast, versatile, and efficient technique for natural product analysis, since many more ions are observed than with the direct infusion ESI-MS. The MS/MS technique provided information about the component structures, revealing the presence of important bioactive components. The application of DESI-MS imaging may contribute to the improvement identification and characterization of pharmacologically active compounds in phytochemistry. Copyright © 2015 John Wiley & Sons, Ltd.
PMID: 26212168 [PubMed - in process] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - July 29, 2015 Category: Chemistry Authors: Bagatela BS, Lopes AP, Cabral EC, Perazzo FF, Ifa DR Tags: Rapid Commun Mass Spectrom Source Type: research
Germinating Seeds of Citrus aurantium a Good Source of Bioactive Limonoids.
Authors: Ariza MR, Herrador del Pino MM, Barrero AF
Abstract
A simple method to obtain extracts enriched in bioactive limonoids from Citrus aurantium L. seeds has been developed, using solvents of increasing polarity. 1H NMR data from the extracts revealed that the highest amounts of limonoids were present in the t-butylmethylether extract. The comparison between extracts obtained from dormant and germinating seeds showed that the latter contained almost double amounts of limonoids, revealing germinating seeds as an excellent source of those bioactive compounds.
PMID: 26197503 [PubMed - in process]...
Source: Natural Product Communications - July 24, 2015 Category: Biochemistry Tags: Nat Prod Commun Source Type: research
Activity antifungal of the essential oils; aqueous and ethanol extracts from Citrus aurantium L.
Abstract
Our study is about the essential oil of Citrus aurantium L. in Tunisia and its plant extract. The yield of this essential oil is 0, 56% but the yield of the extract of plant was 17.1% for the aqueous extract ant 18.3% for the ethanolic extract. The analysis of chemical composition by using GC and GC/MS showed the essential oil of C. aurantium L. species to be rich in monoterpenes such as α-terpineol, lianolyl acetate, linalool and limonene. The antifungal activity of this oil showed us an inhibition of the germination of mushrooms, in the same way we could note that the biologic activities are ge...
Source: Natural Product Research - July 24, 2015 Category: Biochemistry Authors: Metoui N, Gargouri S, Amri I, Fezzani T, Jamoussi B, Hamrouni L Tags: Nat Prod Res Source Type: research
Deep sequencing and analysis of small RNAs in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in Sicily
Abstract
Two representative isolates of a citrus tristeza virus population in Sicily, SG29 (aggressive) and Bau282 (mild), were sequenced via viral small RNAs (vsRNA) produced in budlings of sweet orange grafted on sour orange. Phylogenetic relationships with Mediterranean and exotic isolates revealed that SG29 clustered within the “VT-Asian” subtype, whereas Bau282 belonged to the cluster T30. The study confirms that molecular data need to be integrated with bio-indexing in order to obtain adequate information for risk assessment. (Source: Archives of Virology)
Source: Archives of Virology - July 15, 2015 Category: Virology Source Type: research
Comparative pharmacokinetics and brain distribution of magnolol and honokiol after oral administration of Magnolia officinalis cortex extract and its compatibility with other herbal medicines in Zhi-Zi-Hou-Po Decoction to rats.
This article is protected by copyright. All rights reserved.
PMID: 26173910 [PubMed - as supplied by publisher] (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - July 14, 2015 Category: Biomedical Science Authors: Liu Y, Wang D, Yang G, Shi Q, Feng F Tags: Biomed Chromatogr Source Type: research
A polymethoxy flavonoids‐rich Citrus aurantium extract ameliorates ethanol‐induced liver injury through modulation of AMPK and Nrf2‐related signals in a binge drinking mouse model
Nobiletin and tangeretin are polymethoxy flavonoids (PMFs), found in rich quantities in the peel of citrus fruits. In the present study, we assessed the biological effect of the PMFs on liver damage using a mouse model of binge drinking. First, we extracted PMFs from the peels of Citrus aurantium to make Citrus aurantium extract (CAE). Male C57BL/6 mice were orally treated with silymarin and CAE (50, 100, and 200 mg/kg) for 3 days prior to ethanol (5 g/kg, total of 3 doses) oral gavage. Liver injury was observed in the ethanol alone group, as evidenced by increases in serum hepatic enzymes and histopathologic alterat...
Source: Phytotherapy Research - July 1, 2015 Category: Biochemistry Authors: Bong‐Keun Choi, Tae‐Won Kim, Dong‐Ryung Lee, Woon‐Ha Jung, Jong‐Hwan Lim, Ju‐Young Jung, Seung Hwan Yang, Joo‐Won Suh Tags: Research Article Source Type: research
Larvicidal activity of essential oils of Citrus sinensis and Citrus aurantium (Rutaceae) cultivated in Morocco against the malaria vector Anopheles labranchiae (Diptera: Culicidae)
Conclusions These results suggest that the essential oils isolated from Citrus plants have the potential to be used as an ideal ecofriendly approach for the control An. labranchiae. (Source: Asian Pacific Journal of Tropical Disease)
Source: Asian Pacific Journal of Tropical Disease - May 8, 2015 Category: Tropical Medicine Source Type: research
Identification of the absorbed components and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma by ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry
Publication date: 10 July 2015 Source:Journal of Pharmaceutical and Biomedical Analysis, Volume 111 Author(s): Heyun Zhu , Kaishun Bi , Fei Han , Jiao Guan , Xiaoshu Zhang , Xinjuan Mao , Longshan Zhao , Qing Li , Xiaohong Hou , Ran Yin Zhi-Zi-Da-Huang decoction (ZZDHD), consisting of Gardenia jasminoides Ellis, Rheum palmatum L., Citrus aurantium L. and Sojae Semen Praeparatum, is a widely used traditional Chinese medicine preparation for the treatment of acute or chronic hepatic diseases. In the present study, a sensitive and selective ultra-high performance liquid chromatography coupled with quadrupole-tim...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 3, 2015 Category: Drugs & Pharmacology Source Type: research
Molecules, Vol. 20, Pages 6925-6940: Identification of the Chemical Constituents in Aqueous Extract of Zhi-Qiao and Evaluation of Its Antidepressant Effect
The immature fruit of Citrus aurantium L. (Zhi-Qiao, ZQ) has been used as a traditional medicine in China. Our previous study has shown that ZQ decoction may contribute to the antidepressant-like action of Chaihu-Shu-Gan-San. However, there are no reports on the chemical constituents of ZQ aqueous extract or its anti-depression effects. Firstly, this research reported the on-line identification of the chemical constituents in the aqueous extract of ZQ by coupling ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 31 chemical constituents were identified in ZQ aqueous extra...
Source: Molecules - April 16, 2015 Category: Chemistry Authors: Ming WuHongwu ZhangChao ZhouHongmei JiaZhuo MaZhongmei Zou Tags: Article Source Type: research
Membrane association of a nonconserved viral protein confers virus ability to extend its host range.
In this study, we examined some of the characteristics of p33. We show that p33 is a membrane-associated protein that is inserted into the membrane via a transmembrane helix formed by hydrophobic amino acid residues at the C-terminal end of the protein. Removal of this transmembrane domain (TMD) dramatically altered the intracellular localization of p33. Moreover, the TMD alone was sufficient to confer membrane localization of an unrelated protein. Finally, a CTV variant that produced a truncated p33 lacking the TMD was unable to infect sour orange, one of the selected virus hosts, which infection requires p33, suggesting ...
Source: Virology - April 13, 2015 Category: Virology Authors: Kang SH, Bak A, Kim OK, Folimonova SY Tags: Virology Source Type: research
Analgesic and anti-inflammatory activities of Citrus aurantium L. blossoms essential oil (neroli): involvement of the nitric oxide/cyclic-guanosine monophosphate pathway
Abstract
The analgesic and anti-inflammatory properties of Citrus aurantium L. blossoms essential oil (neroli) were investigated in mice and rats. The analgesic activity of neroli was assessed by acetic acid-induced writhing and Eddy’s hot plate methods, while acute and chronic anti-inflammatory effects were investigated by inflammatory paw edema in rat and the cotton pellet-induced granuloma tissue model, respectively. Mechanistic studies were conducted using L-nitro arginine methyl ester (L-NAME), an inhibitor of NO synthase. Neroli significantly decreased the number of acetic acid-induced writhes in ...
Source: Journal of Natural Medicines - March 12, 2015 Category: Drugs & Pharmacology Source Type: research
Citrus aurantium increases seizure latency to PTZ induced seizures in zebrafish thru NMDA and mGluR's I and II
Coral Rosa-Falero, Stephanie Torres-Rodríguez, Claudia Claudia Jordán, Rígel Licier, Yolimar Santiago, Zuleyma Toledo, Marely Santiago, Kiara Serrano and José G. Ortiz (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - February 13, 2015 Category: Drugs & Pharmacology Source Type: research
