Stilbenoids isolated from the roots of Rheum lhasaense under the guidance of the acetylcholinesterase inhibition activity
AbstractFour unknown stilbenoids, including one dimer, namely 4 ′-methoxy-scirpusin A (5) and three monomeric stilbene glycosides, namely piceatannol-3 ′-O-[2 ′′-(3,5-dihydroxy-4-methoxybenzoyl)]-β-d-glucopyranoside (13), piceatannol-3 ′-O-(2 ′′-galloyl)-β-d-glucopyranoside (14) and piceatannol-3 ′-O-(6 ″-p-coumaroyl)- β-d-glucopyranoside (16) together with 15 described compounds, were isolated from the ethyl acetate fraction of the ethanol extract of roots ofRheum lhasaense based on the guidance of the inhibitory effect on acetylcholinesterase. The struc...
Source: Journal of Natural Medicines - January 7, 2021 Category: Drugs & Pharmacology Source Type: research

Complanadine F, a novel dimeric alkaloid from Lycopodium complanatum
AbstractA novelLycopodium alkaloid, complanadine F (1),seco-complanadine A type was isolated from the club mossLycopodium complanatum. The planar structure and relative configuration were elucidated based on spectroscopic data.Graphic abstract (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - January 6, 2021 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic study of traditional Japanese Kampo medicine shimotsuto used to treat gynecological diseases in rats
This study also performed nontargeted LC–MS/MS analysis of plasma following administration of constituent crude drugs of shimotsuto to find extensively blood-absorbed ingredients of shimotsuto. Among detected peaks in the nontargeted analysis, two peaks could be identified as bergapten and 8-debenzoylpaeoniflorin, subsequently their concentrations in shimotsuto-treated rat plasma were quantified. These pharmacokinetic studies indicated that catalpol showed the highest plasma concentration following administration of shimotsuto, followed by 8 -debenzoylpaeoniflorin. This study suggests that all nine ingredients are ab...
Source: Journal of Natural Medicines - January 4, 2021 Category: Drugs & Pharmacology Source Type: research

One-pot enantioselective synthesis of ( S )-spirobrassinin and non-natural ( S )-methylspirobrassinin from amino acids using a turnip enzyme
AbstractThe enantioselective synthesis of (S)-( −)-spirobrassinin, which features a unique sulfur-containing spirooxindole skeleton, was achieved by focusing on the phytoalexin generation in Brassicaceae plants. Specifically, (S)-( −)-spirobrassinin was obtained in a one-pot fashion froml-tryptophan through a reaction involvingS-spirocyclization with various turnip enzymes and constituents, i.e., using the turnip as a reaction reagent, catalyst, and reaction vessel. Surprisingly, this strategy also enabled the one-pot enantioselective synthesis of the novel non-natural spirooxindole (S)-( −)-5-methylspiro...
Source: Journal of Natural Medicines - January 3, 2021 Category: Drugs & Pharmacology Source Type: research

Effects of clovamide and its related compounds on the aggregations of amyloid polypeptides
AbstractAlzheimer's disease (AD) and type 2 diabetes (T2D) are common diseases in the elderly, and the increasing number of patients with these diseases has become a serious health problem worldwide. The aggregation and development of plaque of amyloid polypeptides (amyloid β; Aβ and human islet amyloid polypeptide; hIAPP, amylin) are found in the brains of patients with AD and the pancreas of patients with T2D and are considered to be, in part, the causes of both diseases, respectively. Therefore, preventing amyloid aggregation may be a promising therapeutic strateg y for preventing AD and T2D. In addition, the ...
Source: Journal of Natural Medicines - January 3, 2021 Category: Drugs & Pharmacology Source Type: research

Development and validation of an analysis method for pesticide residues by gas chromatography –tandem mass spectrometry in Daikenchuto
In this study, we developed an analytical method for 91 compounds, which are listed in USP, using DKT as the subject. The method could extract pesticides from DKT with acetone, elute pesticides with acetonitrile using a SepPak C18 column (5 g) and with ethyl acetate using a DSC-NH2 column (2  g), and perform simultaneous analyses by gas chromatography–tandem mass spectrometry (GC–MS/MS). This method, which could quantify 88 compounds, was validated according to USP. A pesticide residue analysis method that meets USP requirements enables the analysis of pesticide residues with a hig h residue risk and ...
Source: Journal of Natural Medicines - January 3, 2021 Category: Drugs & Pharmacology Source Type: research

Sedative effects of l-menthol, d-camphor, phenylethyl alcohol, and geraniol
AbstractVarious essential oils from plants and fragrance components such as monoterpenes have been discovered to reduce spontaneous movements in mice; thus, it has been made clear that the odor itself has the sedative activity. In the present study, we examined the sedative activity of the odors of fragrance components added to eye drops; l-menthol, d-camphor, phenylethyl alcohol, and geraniol, which are often used as refreshers or preservatives. Each fragrance component was administered by the inhalation route to mice, and the sedative effects were evaluated using an open field test. The results showed that four component...
Source: Journal of Natural Medicines - January 2, 2021 Category: Drugs & Pharmacology Source Type: research

Application of the highly sensitive labeling reagent to the structural confirmation of readily isomerizable peptides
AbstractThioamycolamide A (1) is a biosynthetically unique cytotoxic cyclic microbial lipopeptide that bears ad-configured thiazoline, a thioether bridge, a fatty acid side chain, and a reduced C-terminus. It has gained attention for its unique structure, and very recently we reported the total synthesis of1 via a biomimetic route. The NMR spectra of synthetic1 agreed with those of natural1. However, structural identity between peptidic natural and synthetic compounds is often difficult to confirm by comparison of NMR spectra because their NMR spectra vary depending on the conditions in the NMR tube, which often result in ...
Source: Journal of Natural Medicines - January 2, 2021 Category: Drugs & Pharmacology Source Type: research

Genomics-directed activation of cryptic natural product pathways deciphers codes for biosynthesis and molecular function
AbstractNatural products, which can be isolated from living organisms worldwide, have played a pivotal role in drug discovery since ancient times. However, it has become more challenging to identify a structurally novel molecule with promising biological activity for pharmaceutical development, mainly due to the limited methodologies for their acquisition. In this review, we summarize our recent studies that activate the biosynthetic potential of filamentous fungi by genetic engineering to harness the metabolic flow for the efficient production of unprecedented natural products. The recent revolution in genome sequencing t...
Source: Journal of Natural Medicines - December 3, 2020 Category: Drugs & Pharmacology Source Type: research

Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus
AbstractSix new triterpene saponins (1–5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactusStenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer ’s disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (Aβ) aggregation using thioflavin-T assay, and triterpenes as an aglycone moiety and an alkaline hydrolysate of the saponins were also evaluated. One saponin, stenoside A (7)...
Source: Journal of Natural Medicines - November 24, 2020 Category: Drugs & Pharmacology Source Type: research

Astragaloside IV  inhibits cell proliferation and metastasis of breast cancer via promoting the long noncoding RNA TRHDE-AS1
In this study, we first demonstrated that the lncRNA of TRHDE antisense RNA 1 (TRHDE-AS1) was downregulated in breast cancer tissues and cells. Low TRHDE-AS1 expression is associated with poor outcomes in patients with breast cancer and potentially contributes to the aggressive tumor biology of breast cancer. Furthermore, ASV significantly increased TRHDE-AS1 expression in a dose- and time-dependent manner in breast cancer cells. By upregulating TRHDE-AS1, ASV repressed breast cancer cell growth and metastasis both in vitro and in vivo. Taken together, our data indicated that TRHDE-AS1 participates in the anticancer role o...
Source: Journal of Natural Medicines - November 20, 2020 Category: Drugs & Pharmacology Source Type: research

Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice
This study was conducted to investigate whether and howJumihaidokuto (JHT), a traditional Chinese medicine, prevents UVB-induced skin damage in male HR-1 hairless mice. JHT has been traditionally prescribed for patients presenting skin disorders with redness and swelling, and, in Japan, it is approved for prescription to patients with acute and/or purulent skin disorders, hives, acute eczema, and athlete ’s foot. Considering the traditional use of JHT, we hypothesized that oral administration of JHT might emerge as an effective strategy to prevent UVB-induced skin damage, such as edema and erythema. Here, we pretreat...
Source: Journal of Natural Medicines - November 17, 2020 Category: Drugs & Pharmacology Source Type: research

4 β-Hydroxywithanolide E and withanolide E from Physalis peruviana L. inhibit adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion
AbstractObesity is a risk factor for many diseases, including type 2 diabetes and cardiovascular disease, and is related to the rising morbidity and mortality. Discovery of agents targeting adipogenesis, especially from natural sources, is important for the treatment of obesity. Here, we aimed to identify anti-adipogenic substances in methanol extracts ofPhysalis peruviana and to investigate their effect, along with underlying mechanisms. Activity-guided fractionation of the extract revealed 4 β-hydroxywithanolide E (HWE) and withanolide E (WE) as the adipogenesis inhibitors. Both compounds suppressed mRNA expression ...
Source: Journal of Natural Medicines - November 16, 2020 Category: Drugs & Pharmacology Source Type: research

Isoorientin exerts a urate-lowering effect through inhibition of xanthine oxidase and regulation of the TLR4-NLRP3 inflammasome signaling pathway
AbstractIsoorientin (ISO), a natural flavonoid compound, has been identified in several plants and its biological activity is determined and the study on lowering uric acid has not been reported. In view of the current status of treatment of hyperuricemia, we evaluated the hypouricemic effects of ISO in vivo and in vitro, and explored the underlying mechanisms. Yeast extract-induced hyperuricemia animal model as well as hypoxanthine and xanthine oxidase (XOD) co-induced high uric acid L-O2 cell model and enzymatic experiments in vitro were selected. The XOD activity and uric acid (UA) level were inhibited after the treatme...
Source: Journal of Natural Medicines - November 13, 2020 Category: Drugs & Pharmacology Source Type: research

Costunolide and dehydrocostuslactone from Saussurea lappa root inhibit autophagy in hepatocellular carcinoma cells
In this study, we explored the effects of costunolide (CL) and dehydrocostuslactone (DCL), which are bioactive sesquiterpene lactones in this extract, on autophagy modulation in HepG2 cells. An analysis of autophagy-related proteins demonstrated that CL and DCL blocks the autophagy process that leads to the accumulation of microtubule-associated protein 1 light chain 3 (LC3) and SQSTM1/p62 (p62). LC3 turnover assays indicated that CL and DCL trigger autophagy inhibition by blocking the autophagic flux, thereby resulting in the accumulation of LC3-II and p62. These results are encouraging and warrant further study of CL and...
Source: Journal of Natural Medicines - November 6, 2020 Category: Drugs & Pharmacology Source Type: research

Environmental and soil characteristics in Ephedra habitats of Uzbekistan
AbstractIn Uzbekistan,Ephedra distachya L.,E. equisetina Bunge,E. foliata Boiss. ex C. A. Mey.,E. lomatolepis Schrenk, andE. strobilacea Bunge show species specificity for habitat environments and physical and chemical characteristics of habitat soils. Furthermore, the relationship between soil characteristics and ephedrine and pseudoephedrine contents was examined.E. distachya was found growing from 80 to 200  m above sea level (a.s.l) in the Plateau Ustyurt on the desert steppe of cliffs on soil having relatively higher loss on ignition (19.8–33.8%) and water-soluble cations (Ca2+, 5.14 –133.13; Mg2+, 0....
Source: Journal of Natural Medicines - October 22, 2020 Category: Drugs & Pharmacology Source Type: research

Four new pyrrole alkaloids from the rhizomes of Amomum koenigii
AbstractFour new pyrrole alkaloids, named amokoens A –D (1–4), together with three known compounds (5–7) were isolated from the rhizomes ofAmomum koenigii. Their structures and absolute configurations were established by spectroscopic data, including 1D and 2D NMR, and the optical rotation calculations. All the isolates were evaluated for their effects on nitric oxide (NO) production in lipopolysaccharide-induced RAW264.7 macrophages. Compounds1–7 inhibited NO production ranging from 27.1 to 82.4% at a concentration of 100  μM, and compounds5 and6 showed efficacious inhibitory activities wit...
Source: Journal of Natural Medicines - October 21, 2020 Category: Drugs & Pharmacology Source Type: research

Changes in the extracted amounts and seasonally variable constituents of Diospyros kaki at different growth stages
In this study, by focusing on the medicinal part of Persimmon Calyx, calyx on mature fruit ofD. kaki, we examined the changes in the extraction amounts of 3 cultivars ofD. kaki ( ‘Hiratanenashi’, ‘Jiro’, and ‘Tonewase’) to identify and quantify seasonally variable constituents during the maturation process by analysing their chemical compositions. We found that the extraction weight of the calyx, fruit of persimmons, and total tannin content in calyxes were signi ficantly increased during maturation. Lupeol (1), betulinic acid (2), pomolic acid (3), ursolic acid (4), β-sitosterol (5...
Source: Journal of Natural Medicines - October 21, 2020 Category: Drugs & Pharmacology Source Type: research

Anthraquinone-containing compound in rhubarb prevents indole production via functional changes in gut microbiota
In this study, we developed an optimized HPLC method for analyzing indole production and evaluated the effect of diets and rhubarb on indole production via the changes of gut microbiota. In high-carbohydrate and high-fat diet-fed mice, the indole production was significantly higher than in high-fiber diet-fed mice. We further used the high-carbohydrate diet-fed mice as a model for examining the effect of rhubarb on indole production. The 20% methanol-eluted fraction of aqueous rhubarb extract significantly suppressed indole production, and the eluate constituent rhein 8-O-β-d-glucopyranoside (RG) contributed to this e...
Source: Journal of Natural Medicines - October 19, 2020 Category: Drugs & Pharmacology Source Type: research

Acacienone, a terpenoid-like natural product having an unprecedented C20 framework isolated from Acacia mangium leaves
AbstractA novel C20 natural product, acacienone (1), was isolated from the leaves ofAcacia mangium collected in Bangladesh. The structure of compound1 was elucidated by spectral studies and X-ray crystallographic analysis. Acacienone (1) possesses a terpenoid-related tetracyclic framework containing 20 carbons with biogenetically unusual structural features: (i) vicinal C1-branches at the C-3 and C-4 positions in the A ring, and (ii) a cyclopentenone D ring in an androsterone-like assembly, lacking a methyl group at the C-13 position. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - October 13, 2020 Category: Drugs & Pharmacology Source Type: research

Epoxyquinophomopsins A and B from endophytic fungus Phomopsis sp. and their activity against tyrosine kinase
AbstractTwo new quinone derivatives, epoxyquinophomopsins A (1) and B (2), were purified from the EtOAc extract of endophytic fungusPhomopsis sp isolated fromMorus cathayana. The structures of both compounds were determined based on 1D and 2D NMR and mass spectral data, as well as by x-ray diffraction analysis for1. Compounds1 and2 were screened against eight receptor- (RTKs) and eight non-receptor tyrosine kinases (nRTKs). Both compounds showed strong inhibitory properties against Bruton ’s Tyrosine Kinase (nRTK) with their kinase activity were 19% and 20%, respectively. Only compound 1 that showed strong inhibitory...
Source: Journal of Natural Medicines - October 7, 2020 Category: Drugs & Pharmacology Source Type: research

Structure and anticancer activity of a new lectin from the cultivated red alga, Kappaphycus striatus
AbstractThe red algaKappaphycus striatus is economically important food species and extensively cultivated throughout most tropical parts of the world as a source of carrageenan. In this note, the primary structure of a new lectin KSL from this alga was elucidated by the rapid amplification method of complementary DNA (cDNA) ends, which consists of 267 amino acid residues distributed in four tandem-repeated domains of about 67 amino acids and sharing 43% of identity. The calculated molecular mass from the deduced sequence was consistent with that of natural KSL (27,826  Da) determined by electron spray ionization&ndas...
Source: Journal of Natural Medicines - October 5, 2020 Category: Drugs & Pharmacology Source Type: research

Correction to: Inhibiting reactive oxygen species-dependent autophagy enhanced baicalein-induced apoptosis in oral squamous cell carcinoma
In the original publication of the article, an incorrect immunofluorescence of GFP-LC3 puncta was included in the Fig. 3a. The correct version of Fig. 3a is provided below. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - October 5, 2020 Category: Drugs & Pharmacology Source Type: research

Shenyan Kangfu tablet alleviates diabetic kidney disease through attenuating inflammation and modulating the gut microbiota
This study further observed that SYKFT increased relative abundance of Firmicutes and decreased Bacteroidetes, showing direct cor relation between representative microbiota relative abundance and renal function parameters. SYKFT effectively decreased the relative abundance of Bacteroides, and positive correlation between the relative abundance and protein expression of NF-κB, TNF-α and IL-1β predicted that anti-inflammatory activity of SYKFT was associated with modulating the gut microbiota. Therefore, we first demonstrated SYKFT alleviated DKD through regulating renal inflammatory signaling cascades and i...
Source: Journal of Natural Medicines - September 29, 2020 Category: Drugs & Pharmacology Source Type: research

Diosgenin content is a novel criterion to assess memory enhancement effect of yam extracts
In this study, we aimed to investigate whether one of the main and brain-penetrating components in yams, diosgenin, can be a novel criterion to assess memory enhancement effect of yam extracts. Although our previous studies showed that administration of diosgenin or diosgenin-rich yam extract enhanced cognitive function in normal mice and healthy humans, we have never evaluated whether the effect depends on diosgenin content or not. Therefore, we compared memory enhancement effects of low diosgenin-contained general yam water extract with diosgenin-rich yam extract on cognitive function in normal mice. We found that unlike...
Source: Journal of Natural Medicines - September 25, 2020 Category: Drugs & Pharmacology Source Type: research

Macrocarquinoids A –C, new meroterpenoids from Sargassum macrocarpum
This study was conducted as a search for an AGE inhibitor from brown alga,Sargassum macrocarpum. Separation and purification were performed using AGEs inhibitory activity as an index, yielding isolation of 11 meroterpenoids, of which 3 were new compounds: macrocarquinoids A (1), B (6), and C (9). Their structures were elucidated using NMR spectral analysis with 2D techniques. All tested compounds showed AGEs inhibitory activity. Particularly, macrocarquinoid C (9) possessed the strongest activity (IC50: 1.0  mM) of isolated compounds. This activity was stronger than that of aminoguanidine (positive control).Graphic ab...
Source: Journal of Natural Medicines - September 23, 2020 Category: Drugs & Pharmacology Source Type: research

Two new rearranged clerodane diterpenes from Thai Tinospora baenzigeri
AbstractA new rearranged clerodane-type diterpene (1), tinobaenzigeride, and a new rearranged clerodane glucoside (2) were isolated from the stems ofTinospora baenzigeri, along with four known compounds (3‒6). Their structures were elucidated by spectroscopic data analysis. In addition, the structure and configuration of1 was confirmed by single-crystal X-ray diffraction analysis. Compound1 are a rare example of rearranged clerodanes, since it contains a fully oxygenated tetrahydrofuran moiety. The isolated compounds were evaluated for their cytotoxicity against Hep-G2 and MCF-7 cancer cells, none of them did show any si...
Source: Journal of Natural Medicines - September 23, 2020 Category: Drugs & Pharmacology Source Type: research

PTP1B and α-glucosidase inhibitors from Selaginella rolandi-principis and their glucose uptake stimulation
AbstractAs part of an ongoing search for new protein tyrosine phosphatase 1B inhibitors and glucose uptake stimulators from nature, a new coumarin, selaginolide A (1) and four known isoflavones (2‒5) were isolated from the ethanol extract of a Vietnamese medicinal plantSelaginella rolandi-principis. The chemical structures of the isolates were elucidated by extensive analysis of spectroscopic and physicochemical data. Compounds3‒5 have been identified fromSelaginella genus for the first time. The antidiabetic properties of the isolates (1‒5) were investigated using in vitro assay on 2-NBDG uptake in 3T3-L1 adipocytes...
Source: Journal of Natural Medicines - September 13, 2020 Category: Drugs & Pharmacology Source Type: research

EGCG down-regulates MuRF1 expression through 67-kDa laminin receptor and the receptor signaling is amplified by eriodictyol
Abstract( –)-epigallocatechin-3-O-gallate (EGCG) is a bioactive polyphenol in green tea. Previous studies have demonstrated the beneficial effects of EGCG on muscle mass and muscle atrophy. In the current study, we investigated the mechanisms underlying effect of EGCG on muscle atrophy. It was demonstrated that EGCG suppressed muscle-specific ubiquitin ligase, muscle RING Finger 1 (MuRF1) expression through 67-kDa laminin receptor (67LR). Previous studies have shown that eriodictyol potentiates the anti-tumor activities of EGCG by amplifying 67LR signaling. Therefore, we investigated the effects of EGCG and eriodicty...
Source: Journal of Natural Medicines - August 30, 2020 Category: Drugs & Pharmacology Source Type: research

Correction to: Resveratrol oligomer structure in Dipterocarpaceaeous plants
The article Resveratrol oligomer structure in  Dipterocarpaceaeous plants (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 30, 2020 Category: Drugs & Pharmacology Source Type: research

Correction to: Enzymatic studies on aromatic prenyltransferases
The article Enzymatic studies on aromatic prenyltransferases, written by Takahiro  Mori. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 30, 2020 Category: Drugs & Pharmacology Source Type: research

Solubility enhancement of berberine –baicalin complex by the constituents of Gardenia Fruit
In this report, through solubility-enhancement assay based on HPLC, we identified crocins as the constituents of Gardenia Fruits, which enhanced the solubility of berberine–baicalin comple x. All-trans crocin-1 (1) and 13-cis crocin-1 (5) showed high activities among the isolated crocins, and the number of glucosyl groups in the molecule seemed correlated with the activity. As berberine and baicalin were reported as the anti-inflammatory constituents of Coptis Rhizome and Phellodendron Bark, and Scutellaria Root, respectively, Gardenia Fruit contributes anti-inflammatory activity of orengedokuto by increasing solubil...
Source: Journal of Natural Medicines - August 27, 2020 Category: Drugs & Pharmacology Source Type: research

Iridoids isolated from Viticis Fructus inhibit paclitaxel-induced mechanical allodynia in mice
This study provides confirmatory evidence for the anti-allodynic activity of purified1 and also reveals two additional active iridoids from Viticis Fructus. These three iridoids could be potential candidates for the treatment of CIPN. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 19, 2020 Category: Drugs & Pharmacology Source Type: research

Correction to: The search for, and  chemistry and mechanism of, neurotrophic natural products
he article The search for, and  chemis (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 19, 2020 Category: Drugs & Pharmacology Source Type: research

Structural revision of sesbagrandiflorains A and B, and synthesis and biological evaluation of 6-methoxy-2-arylbenzofuran derivatives
AbstractSesbagrandiflorains A (1) and B (2), isolated from the stem bark of the Indonesian fabaceous plantSesbania grandiflora, were reported to be 6-methoxy-2-(2 ´,3´-dihydroxy-5´-methoxyphenyl)-1-benzofuran-3-carbaldehyde and 6-hydroxy-2-(2´,3´-dihydroxy-5´-methoxyphenyl)-1-benzofuran-3-carbaldehyde, respectively. However, based on reevaluation of their 1D and 2D NMR data, the chemical structures of 1 and 2 have been revised to 4-hydroxy-2-(4´-hydrox y-2´-methoxyphenyl)-6-methoxybenzofuran-3-carbaldehyde and 4-hydroxy-2-(4´-hydroxy-2´-hydroxyphenyl)-6-methoxyben...
Source: Journal of Natural Medicines - August 17, 2020 Category: Drugs & Pharmacology Source Type: research

Kamiohnoyneosides A and B, two new polyacetylene glycosides from flowers of edible Chrysanthemum & quot;Kamiohno & quot;
AbstractTwo new polyacetylene glycosides, kamiohnoyneosides A and B, were isolated from the flowers of edible Chrysanthemum"Kamiohno", along with a known polyacetylene glycoside and two known monoterpene glycosides. The structures of new compounds were elucidated on the basis of spectroscopic data. Kamiohnoyneoside A and three known compounds moderately inhibited formation ofNε-(carboxymethyl)lysine, one of the representative advanced  glycation endproducts.Graphic abstract (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 16, 2020 Category: Drugs & Pharmacology Source Type: research

New isoflavone glucosides in yabumame ( Amphicarpaea bracteata (L.) Fernald subsp. edgeworthii (Benth.) H.Ohashi var. japonica (Oliv.) H.Ohashi) and their effect on leukotriene B 4 production in mast cells
In this study, the structures of yabumame isoflavone glucosides were determined and their effect on leukotriene (LT) B4, a chemical mediator of type I allergy, produced in mast cells, was investigated in vitro. Seven compounds were isolated from yabumame. Their structures were determined by spectroscopic and spectrometric analyses, which were genistein-7-O- β-d-glucoside (1), formononetin-7-O-(2 ″-O- β-d-glucosyl)- β-d-glucoside (2), formononetin-7-O- β-d-glucoside (3), biochanin A-7-O-(2 ″-O- β-d-glucosyl)- β-d-glucoside (4), formononetin-7-O-(6 ″-O-malonyl)- β-d-glucos...
Source: Journal of Natural Medicines - August 15, 2020 Category: Drugs & Pharmacology Source Type: research

Triterpenoids from Celastrus orbiculatus Thunb. inhibit RANKL-induced osteoclast formation and bone resorption via c-Fos signaling
AbstractFourteen triterpenes, lup-20(29)-ene-3 β,6β-diol (1), betulin (2), lupeol caffeate (3), 3 β-caffeoyloxylup-20(29)-en-6α-ol (4), betulin-3 β-yl-caffeate (5), 3 β-trans-feruloylbetulin (6), betulinaldehyde 3-caffeate (7), 3-O-trans-caffeoylbetulinic acid (8), dammarenediol II 3-caffeate (9), 12-oleanene-3 β,6α-diol (10), 11 α-hydroxy-3β-amyrin (11), nivadiol (12), 29-hydroxyfriedelin (13), and celastrusin A (14) were isolated fromCelastrus orbiculatus Thunb. and evaluated for their activity on receptor activator of nuclear factor κB ligand (RANKL)-induced ost...
Source: Journal of Natural Medicines - August 9, 2020 Category: Drugs & Pharmacology Source Type: research

Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF- κB signaling pathway activation: an in vivo and in vitro study
AbstractMonosodium urate (MSU)-mediated inflammation is closely related to gouty arthritis (GA). Dioscin, an active ingredient, has been reported to possess anti-inflammatory property. Nevertheless, the role of dioscin in GA and the underlying mechanism have not been fully understood. In the present study, we investigated the anti-inflammatory effect of dioscin on MSU-induced GA through in vivo and in vitro experiments. Histopathological analysis showed that dioscin alleviated the severity of GA concomitant with the lowered uric acid and creatinine levels. Moreover, the increasing IL-1 β, IL-6, and TNF-α levels ...
Source: Journal of Natural Medicines - August 5, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of metabolites in Saposhnikovia divaricata root from Mongolia
AbstractSaposhnikoviae Radix (SR), derived from the dried root and rhizome ofSaposhnikovia divaricata, is a popular crude drug used in traditional Chinese and Japanese medicine. To evaluate the metabolites ofS. divaricata roots from Mongolia and to investigate their geographical variation, we developed the HPLC method, determined the contents of 9 chromones and 4 coumarins, and conducted multivariate statistical analysis. All Mongolian specimens contained prim-O-glucosylcimifugin (1) and 4 ′-O- β-d-glucosyl-5-O-methylvisamminol (3), and their total amount (5.04 –25.06 mg/g) exceeded the criterion assi...
Source: Journal of Natural Medicines - July 31, 2020 Category: Drugs & Pharmacology Source Type: research

Quality evaluation of Angelicae acutilobae radix: individual differences and localization of ( Z )-ligustilide in Angelica acutiloba root
AbstractIt has been difficult to evaluate the quality of Angelicae acutilobae Radix (Toki) because of large differences in the contents of its chemical constituents. In this research, we revealed individual differences and localization of (Z)-ligustilide in Toki cultivated and processed under the same conditions. Thirteen Toki samples (dry weight: 68.2  g–132.3 g) were divided and categorized into 13 parts and the (Z)-ligustilide content of each part was quantified. Total (Z)-ligustilide content ranged from 0.08% to 0.22% and the maximum difference between samples was approximately 2.8-fold. In addition, th...
Source: Journal of Natural Medicines - July 30, 2020 Category: Drugs & Pharmacology Source Type: research

Two new sesquiterpenes from Artemisia annua L.
AbstractInvestigation of the dried whole plants ofArtemisia annua led to the isolation of two new sesquiterpenes, artemanins A (1) and B (2), along with twenty-nine known compounds. The structures of the new compounds were elucidated by spectroscopic and chemical means. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - July 23, 2020 Category: Drugs & Pharmacology Source Type: research

Two new phenylpropanoids from the resin of Styrax tonkinensis (Pierre) Craib ex Hartw
AbstractAbstractTwo new phenylpropanoids, named stytonkinol A (1) and stytonkinol B (2), have been isolated from the resin ofStyrax tonkinensis (Pierre) Craib ex Hartw. Their structures were determined by spectroscopic analysis, including 1D and 2D NMR, and HR-ESI –MS. Two isolated compounds were assayed for cytotoxic activities against five tumor cell lines (HepG-2, A549, Hela, MCF-7, and PC-3) by Cell Counting Kit-8 (CCK-8) test in vitro. The cytotoxic effectiveness observed against Hela, and MCF-7 cell lines of compound1 were superior or similar to the positive control cisplatin (IC50 values of 40.95 and 47.36 &nb...
Source: Journal of Natural Medicines - July 20, 2020 Category: Drugs & Pharmacology Source Type: research

Sauchinone: a prospective therapeutic agent-mediated EIF4EBP1 down-regulation suppresses proliferation, invasion and migration of lung adenocarcinoma cells
AbstractLung adenocarcinoma (LUAD) is the top prevalent histological kind of lung cancer worldwide. Recent evidences have demonstrated that Sauchinone plays an anticancer role in tumor cell invasion and migration. Therefore, we performed this investigation to explain the potential role of Sauchinone in LUAD as well as the potential mechanism involved. Cell counting kit 8 (CCK-8) and transwell experiments were implemented to measure the proliferative, invasive and migratory abilities of LUAD cells. qRT-PCR and Western blot were performed to detect the transfection efficiency of si-EIF4EBP1s. Additionally, Western blot was a...
Source: Journal of Natural Medicines - July 14, 2020 Category: Drugs & Pharmacology Source Type: research

Licoricidin improves neurological dysfunction after traumatic brain injury in mice via regulating FoxO3/Wnt/ β-catenin pathway
In conclusion, Licoricidin exerted neuroprotective effect on TBI model and the effect was possibly due to its antioxidative effect and antiapoptotic effect via regulating the FoxO3/Wnt/β-catenin pathway. Licoricidin may be a candidate drug for TBI therapy. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - July 11, 2020 Category: Drugs & Pharmacology Source Type: research

Isolation and evaluation of cardenolides from Lansium domesticum as Notch inhibitors
In this study, six naturally occurring Notch inhibitors were isolated from the methanol (MeOH) extract ofLansium domesticum using our novel cell-based assay. Hongherin (2), a cardiac glycoside, demonstrated potent Notch inhibitory activity with an IC50 of 0.62  μM and was found to be cytotoxic in HPB-ALL human T cell acute lymphoblastic leukemia cells. Hongherin (2) also induced the differentiation of C17.2 neural stem cells to neurons, causing a 65% increase in differentiation compared to the control. Mechanistically, hongherin (2) reduced the amount of Notch1 (full length) and mastermind-like protein (MAML). This...
Source: Journal of Natural Medicines - July 8, 2020 Category: Drugs & Pharmacology Source Type: research

Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf.
AbstractAG36 is a triterpenoid saponin fromArdisia gigantifolia stapf. Our recent studies proved that AG36 displayed prominent cytotoxicity against breast cancer cells both in vitro and in vivo. However, whether AG36 has antiangiogenic properties is unknown. Therefore, in the present study, we evaluated the antiangiogenic effect of AG36 and the underlying mechanism. The results indicated that AG36 could significantly inhibit the proliferation, migration and invasion of human umbilical vein endothelial cells (HUVEC). Further antiangiogenic molecular mechanism investigation showed that AG36 significantly suppressed phosphory...
Source: Journal of Natural Medicines - July 7, 2020 Category: Drugs & Pharmacology Source Type: research

The search for, and chemistry and mechanism of, neurotrophic natural products
AbstractNeurotrophic factors, now termed neurotrophins, which belong to a class of polypeptidyl agents, have been shown to potentially be beneficial for the treatment of neurodegenerative diseases such as Alzheimer ’s disease, because endogenous neurotrophic factors (NGF, BDNF, NT3, NT4) have been recognized to play critical roles in the promotion of neurogenesis, differentiation, and neuroprotection throughout the development of the central nervous system. However, high-molecular weight proteins are unable to cross the blood–brain barrier and are easily decomposed by peptidase under physiological conditions. T...
Source: Journal of Natural Medicines - July 7, 2020 Category: Drugs & Pharmacology Source Type: research

Anti-inflammatory canthin-6-one alkaloids from the roots of Thailand Eurycoma longifolia Jack
AbstractTwo new canthin-6-one alkaloids, 4,9-dimethoxy-5-hydroxycanthin-6-one (1) and 9-methoxy-(R/S)-5-(1-hydroxyethyl)-canthin-6-one (2), together with fifteen known ones were isolated from the roots of ThailandEurycoma longifolia Jack. Among the known canthin-6-one alkaloids, compounds9 and16 were isolated from theEurycoma genus for the first time. Meanwhile, the nitric oxide (NO) inhibitory activities of all isolates were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 50  µM. Moreover, a dose-dependent experiment was conducted for active compounds1,2,4,6,7,10,12–17 at the concentrati...
Source: Journal of Natural Medicines - July 7, 2020 Category: Drugs & Pharmacology Source Type: research

Firmosides A and B: two new sucrose ferulates from the aerial parts of Silene firma and evaluation of radical scavenging activities
AbstractTwo new tri-ferulates of sucrose, firmosides A and B (1 and2, respectively), together with 18 known compounds (3–20), were isolated from the aerial parts ofSilene firma. The structures of the isolated compounds were elucidated by various spectroscopic methods, including 1D, 2D NMR, and high-resolution electro-spray ionization –mass spectrometry (HR-ESI–MS). All the isolated compounds were evaluated for their free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. As a result, two new compounds (1,2) and11 demonstrated significant radical scavenging activity, implying t...
Source: Journal of Natural Medicines - July 5, 2020 Category: Drugs & Pharmacology Source Type: research