Development of a fast LC-MS/MS protocol for combined measurement of six LSDs on dried blood spot in a newborn screening program
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Emanuela Scolamiero, Bruno Casetta, Sabrina Malvagia, Tetsuo Tanigawa, Giulia Forni, Silvia Funghini, Massimo Mura, Francesca Raspini, Sara Poggiali, Giancarlo la MarcaAbstractNew treatment options and improved strategies for Lysosomal Storage Disorders (LSDs) diagnosis on dried blood spot (DBS) have led to the development of several pilot newborn screening programs.Building on a previously published protocol, we devised a new 6-plex assay based on a single DBS punch incubated into a buffer containing a combina...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 7, 2018 Category: Drugs & Pharmacology Source Type: research

A novel analytical approach towards in-vitro bile acid binding studies to Colesevelam Hydrochloride tablets: An ultra-high performance liquid chromatography tandem mass spectrometric method
The objective of the study was to develop and validate a liquid chromatography tandem mass spectroscopic method for the simultaneous in-vitro estimation of bile acid salts of Glycocholic acid (GC), Glycochenodeoxycholic acid (GCDC) and Taurodeoxycholic acid (TDC) and its application in performing in-vitro binding study with Colesevelam Hydrochloride tablets. The method was developed using C-18 (50 x 4.6 mm, 3 μm) column with detection on negative ion mode and acquisition time of 3.5 min. The calibration range was linear from 0.0002 mM to 0.0065 mM for GC, 0.0002 mM...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 6, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid proteolytic digestion and peptide separation using monolithic enzyme microreactor coupled with capillary electrophoresis
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Mengxia Cheng, Rong Wang, Baofang Zhang, Zhenkun Mao, Zilin ChenAbstractProteolytic digestion and peptide separation are key steps in proteomic study, which has attracted much attention. A novel immobilized trypsin microreactor based on monolithic capillary column was developed for rapid proteolytic digestion in this work. The monolithic support was prepared using itaconic acid as functional monomer within a silanized fused-silica capillary. By taking advantage of itaconic acid with two carboxylic functional gr...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 6, 2018 Category: Drugs & Pharmacology Source Type: research

Distribution of platinum between nuclear and cytosolic fractions – Can subcellular fractionation be performed quantitatively?
In conclusion, a reliable intracellular localization and quantitation of platinum following administration of Cisplatin can be determined by application of the method.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - December 1, 2018 Category: Drugs & Pharmacology Source Type: research

High sensitive LC–MS/MS method for estimation of eluxadoline in human plasma and its application to pharmacokinetic study
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Sireesha Dodda, Ajitha Makula, Srinivasa Rao Polagani, Raj Narayana KandhagatlaAbstractA selective, sensitive and high throughput liquid chromatography – tandem mass spectrometry (LC–MS/MS) method was developed and validated for estimation of eluxadoline in human plasma. Plasma samples of analyte with internal standard (eluxadoline13CD3) were extracted using solid phase extraction on Orochem Panthera Deluxe cartridges. Chromatographic separation was performed on Ace Phenyl column (150 ×...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 29, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography–mass spectrometry-based urinary metabolomics study on a rat model of simulated microgravity-induced depression
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Teng Xu, Cong Lu, Li Feng, Lin-Xi Fan, Jing Sun, Bei Fan, Qiong Wang, Yan Wang, Xin-Min Liu, Feng-Zhong WangAbstractThe pathophysiological mechanism of depression is complex and its etiology remains unclear. Metabolomics can be used to monitor multiple metabolic pathways simultaneously, thereby investigating the mechanisms of depression onset and its regulation. Therefore, we studied the metabolic profile of urine samples from a rat model of depression for identifying potential metabolic biomarkers associated w...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 29, 2018 Category: Drugs & Pharmacology Source Type: research

A Photoelectrochemical sensing strategy based on single-layer MoS2 modified electrode for methionine detection
Publication date: Available online 28 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yanxin Li, Shuyu Mei, Sumin Liu, Xu HunAbstractMoS2, a typical transition metal disulfide, is widely used in the photoelectrochemical (PEC) sensor construction. In general, MoS2 based PEC sensor are “signal-on” strategies. Surprisingly, we discovered that the PEC response of MoS2 was quenched by methionine greatly. Based on this discovery, a reduction PEC sensing strategy utilized MoS2 modified electrode for methionine detection was fabricated for the first time. Experimental factors, such as, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 28, 2018 Category: Drugs & Pharmacology Source Type: research

Analytical methodologies used for the determination of colistin in biological fluids. Is it still a challenge?
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Ioanna Dagla, Evangelia Karkoula, Eirini Baira, Anthony Tsarbopoulos, Evagelos GikasAbstractColistin is a multicomponent polypeptide antibiotic consisting mainly of colistin A and colistin B, produced by selected strains of Bacillus polymyxa var. Colistinus. Only recently, the prodrug of colistin, colistimethate sodium, is widely used as last resort antibiotic for infections caused by resistant gram-negative bacteria. Colistin having been discovered several years ago, has not subjected to the drug development an...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 28, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid Chromatography-Mass Spectrometry-based Urinary Metabolomics Study on a Rat Model of Simulated Microgravity-induced Depression
Publication date: Available online 26 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Teng Xu, Cong Lu, Li Feng, Lin-Xi Fan, Jing Sun, Bei Fan, Qiong Wang, Yan Wang, Xin-Min Liu, Feng-Zhong WangAbstractThe pathophysiological mechanism of depression is complex and its etiology remains unclear. Metabolomics can be used to monitor multiple metabolic pathways simultaneously, thereby investigating the mechanisms of depression onset and its regulation. Therefore, we studied the metabolic profile of urine samples from a rat model of depression for identifying potential metabolic biomarkers associa...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 27, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of an isonicotinylhydrazide derivative, a novel positive inotropic compound, in mouse plasma by LC–MS/MS and its application to a pharmacokinetics study
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Renjie Hui, Yueying Gao, Jingyi Liu, Haili Liu, Chunlei Tang, Bainian FengAbstractHeart failure (HF) is one of the most serious health problems worldwide. A new positive inotropic compound, an isonicotinylhydrazide derivative (AF-HF001) was designed recently. A liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for the determination of the target analyte in mouse plasma. Samples were prepared by one step precipitation with ethyl acetate and stored in acetonitrile. Chromatograph...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 27, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of sensitive and selective quantification of total and free daptomycin in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry
In conclusion, we succeeded to develop a sensitive and selective method using UPLC-MS/MS for quantitative measurement of total and free daptomycin concentrations in plasma. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

A Novel Electrochemical Nanosensor Based on NH2-Functionalized Multi Walled Carbon Nanotubes for the Determination of Catechol-Orto-Methyltransferase Inhibitor Entacapone
In this study, an antiparkinson drug Entacapone (ENP) is electrochemically investigated under optimized conditions using NH2 functionalized multi walled carbon nanotubes (NH2fMWCNT) decorated over glassy carbon electrode (GCE). The surface morphology of the NH2fMWCNT/GCE was probed by scanning electron microscopy (SEM) armed with EDX analysis. Electrochemical impedance spectroscopy (EIS) was employed to investigate the electron transfer capability of modified and bare electrodes. Cyclic voltammetry was used to compare the redox response of ENP on the surface of modified and unmodified electrodes. The influence of interferi...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid discrimination and quantification of isomeric flavonoid-O-diglycosides in Citrus paradisi cv. Changshanhuyou by online extraction–quadrupole time-of flight tandem mass spectrometry
Publication date: Available online 22 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chaoying Tong, Xia Tong, Shuyun Shi, Keke GuoAbstractRapid differentiation, characterization and quantification of isomers from complex mixtures by direct mass spectrometry (MS) remained an analytical challenge due to their similar or identical MS/MS spectra and matrix interferences. Here, we reported a novel online extraction–quadrupole time-of-flight tandem mass spectrometry (OLE–QTOF-MS/MS) system to rapid, efficient and sensitive analysis of interglycosidic linkage isomers (hesperidin and n...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Ion quantification in liposomal drug products using high performance liquid chromatography
Publication date: Available online 22 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jiewei Wu, Rachael M. Crist, Scott E. McNeil, Jeffrey D. ClogstonAbstractA simple, straightforward analytical method based on liquid chromatography has been optimized to quantify total, internal, and external ions in drug-loaded liposomal products. The quantification of ammonium and sulfate ions in Doxil is detailed; although, the methodology has been extrapolated to quantitate a variety of ions, including calcium, acetate, and others in several different liposomal formulations. Total ion concentrations we...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

High sensitive LC-MS/MS method for estimation of Eluxadoline in human plasma and its application to pharmacokinetic study
Publication date: Available online 24 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sireesha Dodda, Ajitha Makula, Srinivasa Rao Polagani, Raj Narayana KandhagatlaAbstractA selective, sensitive and high throughput liquid chromatography – tandem mass spectrometry (LC-MS/MS) method was developed and validated for estimation of eluxadoline in human plasma. Plasma samples of analyte with internal standard (eluxadoline13CD3) were extracted using solid phase extraction on Orochem Panthera Deluxe cartridges. Chromatographic separation was performed on Ace Phenyl column (150 x&thinsp...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as potential plasma biomarkers to evaluate NSAIDs-induced gastric injury by liquid chromatography-tandem mass spectrometry
Publication date: Available online 24 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Soyoung Shin, Hyeon Myeong Jeong, Seung Eun Cheung, Tae Hwan Kim, Subindra Kazi Thapa, Da Young Lee, Chang Ho Song, Jun Young Lim, Sang-Min Cho, Kyu-Yeol Nam, Won-Ho Kang, Youn-Woong Choi, Beom Soo ShinABSTRACTAlthough major adverse effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) are gastric injury, assessment of NSAIDs-induced gastrointestinal adverse effects is mostly dependent on endoscopy due to the lack of plasma biomarkers. Several amino acids associated with collagenase activit...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Exploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: in vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studies
In this study, ethyl acetate, acetone, ethanol and water extracts from flowers, stems and roots of Cistanche phelypaea (L.) Cout were appraised for radical scavenging activity (RSA) towards 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) and superoxide free radicals, and for metal chelating activities on iron and copper ions. The water extracts had the highest antioxidant activity, especially those from roots and flowers, and were further appraised for in vitro inhibition of enzymes implicated on the onset of human ailments, namely acetyl- (AChE) and butyrylcholinesterase (BuChE) for Al...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Potentiometric characterization of iodine-based disinfecting preparations
Publication date: Available online 24 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Waldemar Gottardi, Markus NaglAbstractA potentiometric method is presented that allows to characterize aqueous iodine-based disinfecting solutions and preparations, respectively. By means of three electrodes (iodide-sensitive, platinum redox, and reference electrode), the concentrations of free iodine, iodide, and triiodide were determined. The apposition “free” means iodine not complexed with ligands originating from the organic matrix of the iodophoric iodine source (e.g. povidone iodine). Ba...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Large-scale profiling of carotenoids by using non aqueous reversed phase liquid chromatography – photodiode array detection – triple quadrupole linear ion trap mass spectrometry: Application to some varieties of sweet pepper (Capsicum annuum L.)
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Alessandra Gentili, Chiara Dal Bosco, Salvatore Fanali, Chiara FanaliAbstractAnalysis of carotenoids is very complex and demanding in terms of both separation and detection. In this article, an analytical strategy relying on high-performance liquid chromatography-photodiode array detection-tandem mass spectrometry (HPLC-PAD-MS/MS) is presented for the large-scale screening of these phytochemicals. Separation was realized by means of Non-Aqueous Reversed Phase (NARP) chromatography on a triacontyl stationary phas...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Paeonia arietina and Paeonia kesrounansis bioactive constituents: NMR, LC-DAD-MS fingerprinting and in vitro assays
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Stefania Sut, Gokhan Zengin, Stefano Dall’Acqua, Markéta Gazdová, Karel Šmejkal, Gizem Bulut, Ahmet Dogan, Mehmet Zeki Haznedaroglu, Muhammad Zakariyyah Aumeeruddy, Filippo Maggi, Mohamad Fawzi MahomoodallyAbstractPaeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective eff...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 25, 2018 Category: Drugs & Pharmacology Source Type: research

Validated analytical method to determine new salivary lipid peroxidation compounds as potential neurodegenerative biomarkers
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Carmen Peña-Bautista, Paula Carrascosa-Marco, Camille Oger, Claire Vigor, Jean-Marie Galano, Thierry Durand, Miguel Baquero, Marina López-Nogueroles, Máximo Vento, Ana García-Blanco, Consuelo Cháfer-PericásAbstractLipid peroxidation is closely related to neurodegenerative diseases since brain shows high lipid composition and oxygen consumption. The determination of lipid peroxidation compounds in non-invasive biological samples would help to monitor the patients’ o...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 24, 2018 Category: Drugs & Pharmacology Source Type: research

Reactive intermediates and bioactivation pathways characterization of avitinib by LC–MS/MS: In vitro metabolic investigation
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Mohamed W. Attwa, Adnan A. Kadi, Ali S. AbdelhameedAbstractAvitinib (AC0010) is a third generation inhibitor of the EGFR (epidermal growth factor receptor) that was permitted parallel phase I clinical trials in the US and in 2014. It is estimated to enter in market within two years. In the current study, eight in vitro metabolites were detected and their chemical structures were postulated. The main in vitro phase-I metabolic reaction was N-oxidation in piperazine moiety. The generation of reactive metabolites i...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic studies unveiled the drug–drug interaction between trans-2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucopyranoside and emodin that may contribute to the idiosyncratic hepatotoxicity of Polygoni Multiflori Radix
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Yanchao Xing, Linlin Wang, Chenxi Wang, Youcai Zhang, Yi Zhang, Limin Hu, Xiumei Gao, Lifeng Han, Wenzhi YangAbstractPolygoni Multiflori Radix (PMR) has been a reputable tonifying traditional Chinese medicine for a long history. However, clinical side effects regarding its idiosyncratic hepatotoxicity are occasionally reported. The containing anthraquinones, particularly emodin, could cause liver injury in both in vitro and in vivo experiments. It is well-known that some compounds could influence other compounds...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics, bioavailability and metabolism of cligosiban, an antagonist of oxytocin receptor, in rat by liquid chromatography hyphenated with electrospray ionization tandem mass spectrometry
This study is the first report on the pharmacokinetic and metabolic information of cligosiban, which would provide insights into the effectiveness and toxicity of cligosiban. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research

Authentication and discrimination of green tea samples using UV–vis, FTIR and HPLC techniques coupled with chemometrics analysis
This study aimed to develop a simple method for assessing the quality of green tea samples obtained from the South and the East Asian regions. The UV–vis, FTIR and HPLC data from 38 samples were subjected to multivariate analyses using the unsupervised recognition techniques comprising Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA). The model for their authentication was constructed and validated by applying the supervised recognition techniques as Soft Independent Modeling of Class Analogy (SIMCA) and Partial Least Square Discriminant Analysis (PLS-DA). The percentages of caffeine in the ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research

Distribution of platinum between nuclear and cytosolic fractions - Can subcellular fractionation be performed quantitatively?
In conclusion, a reliable intracellular localization and quantitation of platinum following administration of Cisplatin can be determined by application of the method.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 21, 2018 Category: Drugs & Pharmacology Source Type: research

Preparation from Lepidium meyenii Walpers using high-speed countercurrent chromatography and thermal stability of macamides in air at various temperatures
Publication date: Available online 20 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jia-Lun Zhong, Hao Yan, Hui-Dong Xu, Nadeem Muhammad, Wei-Dong YanAbstractOriginally cultivated in the Peruvian Andes, Maca (Lepidium meyenii) is now widely consumed as functional food which is known for its functional characteristic like relieving fatigue and enhancing stamina. Macamides are the unique fatty acids with long-chain and N-benzylamides among the various constituents extracted from Maca. N-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide, N-benzyl-(9Z, 12Z)-octadecadienamide, N-benzylhexadecanamide an...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 21, 2018 Category: Drugs & Pharmacology Source Type: research

A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties
In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 21, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography–tandem mass spectrometry of recombinant human extracellular superoxide dismutase (rhSOD3) in mouse plasma and its application to pharmacokinetic study
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Jong-Woo Jeong, Ji-Hoon Oh, Yu-Geun Ji, Yu-Mi Shin, Myeong Hwi Lee, Nam Sook Kang, Weontae Lee, Sung-Sub Kim, Tae-Yoon Kim, Tae-Sung KooAbstractThe antioxidant enzyme human extracellular superoxide dismutase (SOD3) is a promising biopharmaceutical candidate for the treatment of various diseases. To support the early development of SOD3 as a biopharmaceutical, a simple, sensitive, and rapid liquid chromatography tandem mass spectrometry procedure was developed and validated for the determination of SOD3 levels in...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 21, 2018 Category: Drugs & Pharmacology Source Type: research

Study on anti-hyperuricemia effects and active ingredients of traditional Tibetan medicine TongFengTangSan (TFTS) by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
Publication date: Available online 17 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hai-fang Chen, Chen Zhang, Yun Yao, Jun-mao Li, Wen-di Du, Mu-lan Li, Bei Wu, Shi-lin Yang, Yu-lin Feng, Wu-gang ZhangAbstractTongFengTangSan (TFTS), a traditional Tibetan medicine comprising of Tinospora sinensis (TS), Terminalia chebula Retz (TC) and Trogopterori faeces (TF), is used to treat joint diseases like gout, gout arthritis, swelling, pain etc. Despite the significant therapeutic effects of TFTS, its pharmacological components and active ingredients have not been analyzed so far. Therefore, the ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 18, 2018 Category: Drugs & Pharmacology Source Type: research

Reactive Intermediates and Bioactivation Pathways Characterization of Avitinib by LC-MS/MS: In vitro Metabolic Investigation
Publication date: Available online 17 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed W. Attwa, Adnan A. Kadi, Ali S. AbdelhameedAbstractAvitinib (AC0010) is a third generation inhibitor of the EGFR (epidermal growth factor receptor) that was permitted parallel phase I clinical trials in the US and in 2014. It is estimated to enter in market within two years. In the current study, eight in vitro metabolites were detected and their chemical structures were postulated. The main in vitro phase-I metabolic reaction was N-oxidation in piperazine moiety. The generation of reactive metabol...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 18, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic studies unveiled the drug-drug interaction between trans-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside and emodin that may contribute to the idiosyncratic hepatotoxicity of Polygoni Multiflori Radix
Publication date: Available online 16 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yanchao Xing, Linlin Wang, Chenxi Wang, Youcai Zhang, Yi Zhang, Limin Hu, Xiumei Gao, Lifeng Han, Wenzhi YangAbstractPolygoni Multiflori Radix (PMR) has been a reputable tonifying traditional Chinese medicine for a long history. However, clinical side effects regarding its idiosyncratic hepatotoxicity are occasionally reported. The containing anthraquinones, particularly emodin, could cause liver injury in both in vitro and in vivo experiments. It is well-known that some compounds could influence other com...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

In vivo phenotyping of cytochrome 450 isoforms involved in the metabolism of anti-HIV and anti-tubercular drugs in human using cocktail approach: an LC-MS/MS analysis
ConclusionsThis study advocates the use of a cocktail of bupropion, losartan and dapsone for in vivo phenotyping of CYP2B6, CYP2C9 and NAT2, which is important in determining therapeutic dose levels of anti-HIV and anti-TB drugs in HIV/AIDS-TB coinfections. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a method to analyze the complexes of enoxaparin and platelet factor 4 with size-exclusion chromatography
Publication date: Available online 16 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fangxia Wu, Kai Dong, Meng Zhu, Qinghua Zhang, Bingying Xie, Duxin Li, Hao Gan, Robert J. Linhardt, Zhenqing ZhangAbstractHeparin, a highly sulfated glycosaminoglycan, has been used as a clinical anticoagulant over 80 years. However, heparin-induced thrombocytopenia and thrombosis (HITT) is a serious side effect of heparin therapy, resulting in relatively high risk of amputation and even death. HITT is caused by forming of complexes between heparin and platelet factor 4 (PF4). Enoxaparin, one of the most c...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

Editorial For Sergio and Sandor
Publication date: Available online 17 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Coral Barbas, Bezhan Chankvetadze, Sandra Furlanetto, Jun Haginaka, Shaoping Li, Ruin Moaddel, Jean-Luc Veuthey (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

Effect of Temperature on the Retention of Janus Kinase 3 Inhibitor in Different Mobile Phase Compositions Using Reversed-Phase Liquid Chromatography
In this study, the thermodynamic dissociation constant (pKa) values of tofacitinib in acetonitrile-water binary mixtures with of 25%, 30%, 35% and 45% (v/v) have been determined at 25-45 °C range of temperatures with reversed-phase liquid chromatography (RPLC). The chromatographic determination was achieved on a Kinetex Core-Shell EVO C18-Phenomenex (150 mm x 4.6 mm, 5 µm) analytical column. For each case pKa values and retention factors of tofacitinib by taking into account the effect of the activity coefficients in hydro-organic water–acetonitrile binary mixtures ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

An integrative urinary metabolomic study of the therapeutic effect of Guizhi Fuling capsule on primary dysmenorrheal rats based 1H NMR and UPLC-MS
In conclusion, the variations in potential biomarkers revealed the therapeutic mechanism of GFC, and these potential biomarkers were both significant for early diagnosis and predicting PD. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

Ginkgo leaf-based synthesis of nitrogen-doped carbon quantum dots for highly sensitive detection of salazosulfapyridine in mouse plasma
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Xiaohua Jiang, Dongmiao Qin, Guichun Mo, Jinsu Feng, Chunhe Yu, Weiming Mo, Biyang DengAbstractA simple, economical hydrothermal strategy for synthesizing nitrogen-doped carbon quantum dots (N-CQDs) was developed using Ginko leaves as a carbon source. These N-CQDs have strong blue fluorescence, excitation-relevant emissions, high monodispersity, good stability, good water solubility, and a 22.8% fluorescence quantum yield. They average 3 nm in size, and have maximum excitation and emission wavelengths of ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 17, 2018 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography-tandem mass spectrometry analysis of recombinant human extracellular superoxide dismutase (rhSOD3) in mouse plasma and its application to pharmacokinetic study
Publication date: Available online 15 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jong-Woo Jeong, Ji-Hoon Oh, Yu-Geun Ji, Yu-Mi Shin, Myeong Hwi Lee, Nam Sook Kang, Weontae Lee, Sung-Sub Kim, Tae-Yoon Kim, Tae-Sung KooAbstractThe antioxidant enzyme human extracellular superoxide dismutase (SOD3) is a promising biopharmaceutical candidate for the treatment of various diseases. To support the early development of SOD3 as a biopharmaceutical, a simple, sensitive, and rapid liquid chromatography tandem mass spectrometry procedure was developed and validated for the determination of SOD3 lev...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of chemical stability of sitagliptin by LC-UV, LC-MS and FT-IR methods
Publication date: Available online 15 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Anna Gumieniczek, Anna Berecka, Tomasz Mroczek, Krzysztof Wojtanowski, Kinga Dąbrowska, Katarzyna StępieńAbstractSitagliptin was stored at high temperature/high humidity, dry hot air, UV/VIS light and different pH. Then, a selective LC-UV method was developed for determination of sitagliptin in the presence of degradation products and for estimation of degradation kinetics. Because parent drugs can react with excipients in final pharmaceutical formulations, stability of sitagliptin was also examined in ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

A fast and simple FIA-chemiluminescence method for the evaluation of Roselle flowers as scavenger of the free radicals generated by UV irradiated antibiotics
Publication date: Available online 15 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): José A. Murillo Pulgarín, Luisa F. García Bermejo, Armando Carrasquero DuránAbstractThis work proposes a new method for the in vitro evaluation of the effect of UV irradiation on the production of free radicals and other reactive species during the photodecomposition of drugs. The method was based on the UV irradiation of antibiotics molecules to generate excited states that undergo to homolytic bond cleavages. These reactive species can be detected by their ability to oxidize t...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Authentication and Discrimination of Green Tea Samples Using UV-Visible, FTIR and HPLC Techniques Coupled with Chemometrics Analysis
This study aimed to develop a simple method for assessing the quality of green tea samples obtained from the South and the East Asian regions. The UV-Visible, FTIR and HPLC data from 38 samples were subjected to multivariate analyses using the unsupervised recognition techniques comprising Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA). The model for their authentication was constructed and validated by applying the supervised recognition techniques as Soft Independent Modeling of Class Analogy (SIMCA) and Partial Least Square Discriminant Analysis (PLS-DA). The percentages of caffeine in the id...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Separation and analysis of flavonoid chemical constituents in flowers of Juglans regia L. by ultra-high-performance liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry
In this study, an analytical methodology for identification of flavonoid chemical constituents in flowers of Juglans regia L. was established using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). Thirty-six flavonoids compounds were identified based on the highly accurate measurements, and the mass spectrometric fragmentation pathways of eleven representative compounds were proposed, which were helpful for the identification of different types of flavonoids. The study has laid a foundation for the research and development of effective drugs from flowers of Juglans ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

An application of QSRR approach and multiple linear regression method for lipophilicity assessment of flavonoids
Publication date: Available online 16 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mariusz Zapadka, Mateusz Kaczmarek, Bogumiła Kupcewicz, Przemysław Dekowski, Agata Walkowiak, Adam Kokotkiewicz, Maria Łuczkiewicz, Adam BucińskiAbstractThe analysis of quantitative structure-retention relationships (QSRR) is useful tool for assessment of compound’s lipophilicity/hydrophobicity due to similarity between its retention in chromatographic system and ability to permeation through biological membranes. The main goal of this study was to compare usefulness of two reversed-phase chromat...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous analysis by LC–MS/MS of 22 ketosteroids with hydroxylamine derivatization and underivatized estradiol from human plasma, serum and prostate tissue
This study describes a validated LC–MS/MS method for assaying 23 steroids within a single run from 150 µl of human plasma, serum or prostatic tissue homogenate. Isotope-labeled steroids were used as internal standards. Samples were extracted with toluene, and ketosteroids were derivatized with hydroxylamine prior to LC–MS/MS analysis. The steroids were separated on a C18 column and methanol was used as an organic solvent with the addition of 0.2 mM ammonium fluoride to improve underivatized estradiol (E2) ionization. Certified reference serums as well as plasma samples, and homogenates of pr...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Comprehensive metabolic profile of phenolic acids and flavonoids in Glechomae Herba using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight tandem mass spectrometry with diagnostic ion filtering strategy
Publication date: Available online 16 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yun Luo, Chang-Jiang-Sheng Lai, Jing Zhang, Yulin Feng, Quan Wen, Ting TanAbstractGlechomae Herba (GH), a traditional Chinese medicine, is commonly used for the treatment of urolithiasis and cholelithiasis based on its diuretic and cholagogic actions. However, relatively few studies have been conducted to describe this medicine’s metabolites. Therefore, an ultra-high-performance liquid chromatography coupled to quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) analysis with diagno...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

LC–MS/MS-based quantification of efflux transporter proteins at the BBB
Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): David Gomez-Zepeda, Meryam Taghi, Maria Smirnova, Philippe Sergent, Wang-Qing Liu, Cerina Chhuon, Michel Vidal, Martin Picard, Elizabeth Thioulouse, Isabelle Broutin, Ida-Chiara Guerrera, Jean-Michel Scherrmann, Yannick Parmentier, Xavier Decleves, Marie-Claude MenetAbstractTargeted protein quantification using tandem mass spectrometry coupled to high performance chromatography (LC–MS/MS) has been used to quantify proteins involved in the absorption, distribution, metabolism and excretion (ADME) of xenobio...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a validated LC-MS/MS method for the in vitro and in vivo quantitation of sunitinib in glioblastoma cells and cancer patients
Publication date: Available online 14 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maria V. Chatziathanasiadou, Evgenios K. Stylos, Efstathia Giannopoulou, Maria-Helen Spyridaki, Evangellos Briasoulis, Haralabos P. Kalofonos, Tim Crook, Nelofer Syed, Gregory B. Sivolapenko, Andreas G. TzakosAbstractSunitinib is a multi-targeted tyrosine kinase inhibitor approved for the treatment of renal cell carcinoma and imatinib-resistant gastrointestinal stromal tumor and is currently being investigated against other forms of malignant tumors. Recently a great interest has been emerged for the appli...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 15, 2018 Category: Drugs & Pharmacology Source Type: research

A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC-MS/MS profiles, antioxidant and enzyme inhibitory properties
In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - November 14, 2018 Category: Drugs & Pharmacology Source Type: research

X-ray irradiation effects on nuclear and membrane regions of single SH-SY5Y human neuroblastoma cells investigated by Raman micro-spectroscopy
Publication date: Available online 12 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): I. Delfino, G. Perna, V. Ricciardi, M. Lasalvia, L. Manti, V. Capozzi, M. LeporeAbstractRaman micro-spectroscopy was performed in vitro on nuclear and membrane regions of single SH-SY5Y human neuroblastoma cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). The acquired spectra were analyzed by principal component analysis (PCA) and interval-PCA (i-PCA) methods. Biochemical changes occurring in the different regions of single cells as a consequence of the radiation exposure were observed ...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 14, 2018 Category: Drugs & Pharmacology Source Type: research