Microwave-assisted digestion using dilute nitric acid solution and investigation of calibration strategies for determination of As, Cd, Hg and Pb in dietary supplements using ICP-MS
This study proposes an analytical procedure for microwave-assisted sample preparation of dietary supplements for athletes using dilute nitric acid solution followed by determination of elemental impurities (As, Cd, Hg and Pb) by inductively coupled plasma mass spectrometry (ICP-MS) according to the United States Pharmacopeia Chapters 2232 and 233. Calibration strategies as internal standardization (IS), multi-isotope calibration (MICal), and one-point standard addition (OP SA) were applied for correction of matrix effects. The optimization of the sample preparation procedure was performed using a Doehlert experimental desi...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 13, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative research on the metabolism of metoprolol by four CYP2D6 allelic variants in vitro with LC-MS/MS
Publication date: Available online 11 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Qingqing Yang, Jing Sun, Chuan Li, Haizhi Zhang, Weiren Xu, Changxiao Liu, Xuemin ZhengAbstractSpecific study about the effect of cytochrome P450 2D6 (CYP2D6) polymorphisms on the metabolism of clinic drugs is of great significance for drug safety investigation. Here, the interaction between CYP2D6 variants (*1, *2, *10, *39) and metoprolol (MET) was intensively researched in vitro from the aspect of drug-enzyme kinetic study. To obtain quantitative data, α-hydroxymetoprolol (main metabolite of MET) was ...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 13, 2019 Category: Drugs & Pharmacology Source Type: research

First profiling in hydrophilic interaction liquid chromatography of intact human chorionic gonadotropin isoforms
In this study, for the first time, the potential of hydrophilic interaction liquid chromatography (HILIC) was investigated to separate the intact hCG isoforms. Three different HILIC stationary phases were tested using an hCG-based drug as standard, a recombinant hCG. For each stationary phase, the effect of the initial mobile phase composition based on ACN/H2O mixture, the slope of the gradient, the content and nature of the acidic additive (formic acid and trifluoroacetic acid (TFA)), and the addition of a volatile salt (ammonium formate) on the retention and the resolution were studied. The best HILIC separation was obta...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 13, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous Quantification of Total Antibody and Antibody-Conjugated Drug for XMT-1522 in Human Plasma Using Immunocapture-Liquid Chromatography/Mass Spectrometry
Publication date: Available online 11 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ling Xu, Zhiling Zhang, Shengsheng Xu, Jian Xu, Zhongping John Lin, David LeeAbstractXMT-1522, an antibody-drug conjugate (ADC) currently in Phase I clinical development, represents the first Dolaflexin®-based, cleavable ADC with a high drug-antibody ratio (DAR). In this work, a novel immunocapture LC-MS/MS method was successfully developed for the simultaneous quantification of both total antibody and cleavable antibody-conjugated drug auristatin F-hydroxypropylamide (AF-HPA) in human plasma. This method ...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 13, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolic characterization of diabetic retinopathy: An 1H-NMR-based metabolomic approach using human aqueous humor
Publication date: Available online 10 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Huiyi Jin, Bijun Zhu, Xia Liu, Jing Jin, Haidong ZouAbstractPatients with a long duration of diabetes mellitus (DM) usually have accompanied complications such as diabetic retinopathy (DR), which is a leading cause of blindness and visual impairment among working-age persons in developed countries; nevertheless, some patients have no complications. Thus, various studies, including genomic, transcriptomic, and proteomic studies, have been conducted to identify potential biomarkers for predicting DR and to revea...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 11, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of the stability indicating RP-UHPLC method for the determination of the chemical purity and assay of bimatoprost
Publication date: Available online 10 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marta ZezulaAbstractA simple, rapid and accurate ultra-high performance liquid chromatographic (UHPLC) method with a UV detection for the determination of the chemical purity and assay of bimatoprost (BT-1) was developed. The chromatographic separation was achieved with the use of an Acquity BEH C8, 150 x 2.1 mm, 1.7 µm reversed phase analytical column. The mobile phase consisted of 0.01% H3PO4 : acetonitrile (initial conditions 80 : 20, v/v) was passed through the column at t...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 11, 2019 Category: Drugs & Pharmacology Source Type: research

Evaluation of the quality of Compound Liquorice Tablets by DSC and HPLC fingerprints assisted with dissolution
Publication date: Available online 10 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lili Lan, Yujing Zhang, Mengting Zhang, Guoxiang SunAbstractA systematic quantitative fingerprint method (SQFM) was applied to evaluate the quality of compound liquorice tablets (CPLTs) in this paper. The method contained three main parameters: macroscopic qualitative similarity (Sm), macro quantitative similarity (Pm) and a leveling variance coefficient (α), which were used to analyze the similarity of curves and evaluate the fingerprints of CPLTs. Firstly, Differential Scanning Calorimetry (DSC) was ap...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 11, 2019 Category: Drugs & Pharmacology Source Type: research

Magnetic dispersive solid-phase micro-extraction combined with high-performance liquid chromatography for determining nucleotides in Anoectochilus roxburghii (Wall.) Lindl
Publication date: Available online 7 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaozhen Chen, Youjia Wu, Liying Huang, Lijuan Yang, Ruixia Hong, Hong Yao, Shaoguang LiAbstractA novel, simple, and efficacious analytical method for determining of nucleotides in Anoectochilus roxburghii (Wall.) Lindl (A. roxburghii) was developed. Magnetic dispersive solid-phase micro-extraction (MDSPME) combined with high-performance liquid chromatography was applied for extraction and determination of three nucleotides, such as adenosine-5ʹ-monophosphate (AMP), uridine-5ʹ-monophosphate (UMP) and guanosin...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 8, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid profiling of alkaloid analogues in Sinomenii Caulis by an integrated characterization strategy and quantitative analysis
In conclusion, the proposed method provided a potential approach for chemical profiling and holistic quality control of herbal medicines.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - June 8, 2019 Category: Drugs & Pharmacology Source Type: research

UPLC/MS/MS assay for the simultaneous determination of seven antibiotics in human serum–Application to pediatric studies
Publication date: 10 September 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 174Author(s): Sophie Magréault, Stephanie Leroux, Jeremie Touati, Thomas Storme, Evelyne Jacqz-AigrainAbstractA rapid and highly sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay was developed for quantification of 7 antibiotics in low sample volumes (50 μL): amoxicillin, azithromycin, cefotaxime, ciprofloxacin, meropenem, metronidazole and piperacillin, for both routine monitoring and pharmacokinetic studies. After protein precipitation by acetonitrile, the antibiotic...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 8, 2019 Category: Drugs & Pharmacology Source Type: research

Liquidambar styraciflua L.: A new potential source for therapeutic uses
Publication date: Available online 6 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Graziele Francine Franco Mancarz, Laressa Carolina Laba, Elaine Cristina Pinto da Silva, Maria Rosa Machado Prado, Lauro Mera de Souza, Daiany de Souza, Tomoe Nakashima, Rosiane Guetter MelloAbstractLiquidambar styraciflua L., ALTINGIACEAE, popularly known as sweet gum or alligator tree, is an aromatic tree with a natural distribution in North America and acclimated in Brazil. In traditional medicine, L. styraciflua L is used for the treatment of stomach disorders, wounds, and coughs. The present study was desi...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 7, 2019 Category: Drugs & Pharmacology Source Type: research

Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspectives
Publication date: Available online 6 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gokhan Zengin, E.J. Llorent-Martínez, Kouadio Ibrahime Sinan, Evren Yıldıztugay, Carene Picot-Allain, Mohammad Fawzi MahomoodallyAbstractEthnobotanical evidences substantiate the use of several Centaurea species to treat and/or manage several human ailments. In the present study, the phytochemical profile of the ethyl acetate, methanol, and aqueous extracts (prepared by infusion and decoction) of Centaurea bornmuelleri Hausskn. aerial parts was established. The enzyme inhibitory and antioxidant propert...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 7, 2019 Category: Drugs & Pharmacology Source Type: research

An improved protocol for ICP-MS-based assessment of the cellular uptake of metal-based nanoparticles
Publication date: Available online 5 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Joanna Kruszewska, Agnieszka Kur, Dominika Kulpińska, Ilona Grabowska-Jadach, Magdalena Matczuk, Bernhard K. Keppler, Andrei R. Timerbaev, Maciej JaroszAbstractAlong with a growing interest in biomedical applications of metal-based nanoparticles, there is a compelling need in systematic information on their behavior in human body systems, preferably at the cellular level. However, in most of the in-vitro uptake experiments, the nanomaterial was applied in its native form that in reality can hardly reach the ce...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 6, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a Chiral LC-ESI-MS/MS method for simultaneous determination of the enantiomeric metabolites Isosorbide 2-Mononitrate and Isosorbide 5-Mononitrate of Isosorbide dinitrate in rat and human plasma
Publication date: Available online 5 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bhavesh D. Patel, Nageswararao Pulakundam, Ajit R. Gadekar, Amol A. Raje, Vikram Ramanathan, Rakshit AmetaAbstractA specific liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) assay was developed and validated for simultaneous determination of two active metabolites of isosorbide dinitrate (ISDN), namely, isosorbide 2-mononitrate (IS 2-MN) and isosorbide 5-mononitrate (IS 5-MN). A simple protein precipitation extraction technique was employed using 13C6 isosorbide 5-mononitrate as the internal standa...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 6, 2019 Category: Drugs & Pharmacology Source Type: research

Lipidomics in autoimmune diseases with main focus on Systemic Lupus Erythematosus
Publication date: Available online 6 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Helena Beatriz Ferreira, Ana Margarida Pereira, Tânia Melo, Artur Paiva, M. Rosário DominguesAbstractAutoimmune diseases (AID) are a heterogeneous group of disorders that have in common a chronic inflammation and dysregulation of the immune system. Systemic lupus erythematosus (SLE) is one of the most frequent systemic autoimmune diseases characterized by autoimmune phenomena in multiple organs. The tests used for evolution and prognosis assessment are either non-specific or non-sensitive, impairin...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 6, 2019 Category: Drugs & Pharmacology Source Type: research

A fully automatic cross used solid-phase extraction online coupled with ultra-high performance liquid chromatography-tandem mass spectrometry system for the trace analysis of multi-class pharmaceuticals in water samples
Publication date: Available online 6 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yanshan Liang, Jiaqi Liu, Qisheng Zhong, Dianbao Yu, Jinting Yao, Taohong Huang, Mingjun Zhu, Ting ZhouAbstractA fully automatic system, which integrated cross used solid-phase extraction with ultra-high performance liquid chromatography-tandem mass spectrometry, was developed and validated for the simultaneous determination of multi-class pharmaceuticals (62 in total) in Milli-Q water, tap water, lake water, and ground water. The online system allowed the cross-utilization of two SPE columns without significan...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 6, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 5 September 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 173Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - June 6, 2019 Category: Drugs & Pharmacology Source Type: research

Enhancing counterfeit and illicit medicines grouping via feature selection and X-ray fluorescence spectrometry
Publication date: 10 September 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 174Author(s): Felipe Soares, Michel J. Anzanello, Flavio S. Fogliatto, Rafael S. Ortiz, Kristiane C. Mariotti, Marco F. FerrãoAbstractIn this paper, we propose a novel framework to select the most relevant X-Ray Fluorescence (XRF) energy values (i.e., features) to enhance the clustering (grouping) of counterfeit and illicit medical tablets. The framework is based on the integration of multidimensional scaling (MDS) and Procrustes analysis (PA) multivariate techniques. MDS provides a projection of the original data in...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 5, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative charge-based separation study with various capillary electrophoresis (CE) modes and cation exchange chromatography (CEX) for the analysis of monoclonal antibodies
Publication date: Available online 3 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julia Kahle, Holger Zagst, Rebecca Wiesner, Hermann WätzigAbstractCharge heterogeneity is an important critical quality attribute for the analysis of monoclonal antibodies (mAbs). For this, (imaged) capillary isoelectric focusing ((i)cIEF), ion exchange chromatography (IEC) and, recently, capillary zone electrophoresis (CZE) are the predominantly used techniques. In order to investigate which one is most suitable to answer a specific analytical question, here, the four aforementioned separation techniques ...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 4, 2019 Category: Drugs & Pharmacology Source Type: research

Investigation of Chemical Profile, Biological Properties of Lotus corniculatus L. Extracts and Their Apoptotic-Autophagic Effects On Breast Cancer Cells
This study aimed to reveal chemical profiles and biological activities of ethyl acetate (EA), methanol (MeOH), and water extracts of Lotus corniculatus. Ethnobotanical reports have indicated the importance of phytochemical properties of the genus Lotus. In this study, the effects of medicinal plant extracts on antioxidant (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays), enzyme inhibitory (on cholinesterase, tyrosinase, a-amylase and a-glucosidase), DNA protection and anticancer properties (including anti-proliferative, cell death and telomerase activity marker gene analysis, apoptotic DNA fragment...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 4, 2019 Category: Drugs & Pharmacology Source Type: research

Detection of sildenafil adulterated in herbal products using thin layer chromatography combined with surface enhanced Raman spectroscopy: “Double Coffee-ring effect” based enhancement
In this study, a rapid, sensitive and selective method using thin layer chromatography (TLC) combined with surface-enhanced Raman spectroscopy (SERS) was developed for identification of sildenafil adulteration in herbal drugs and dietary supplements. TLC separation method was developed and different SERS factors were investigated: nanosilver colloid preparation, concentration and volume, and coffee-ring effect (CRE) enhancement for SERS measurement. “Double CRE” - a newly observed effect that resulted in the redistribution of both silver nanoparticles and sildenafil molecules – was reported for the first ...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 3, 2019 Category: Drugs & Pharmacology Source Type: research

Design of immunogens: the effect of bifunctional chelator on immunological response to chelated copper
In this study, Cu2+ was coupled with carrier proteins via three different macrocyclic BFCs: p-SCN-Bn-DOTA, p-SCN-Bn-NOTA, and p-SCN-Bn-TETA. The stability in plasma and the immunogenicity of three copper immunoconjugates were compared. The chelators other than p-SCN-Bn-DOTA were very stable in plasma, with
Source: Journal of Pharmaceutical and Biomedical Analysis - June 3, 2019 Category: Drugs & Pharmacology Source Type: research

Identification and Analysis of Cardiac Glycosides in Loranthaceae Parasites Taxillus chinensis (DC.) Danser and Scurrula parasitica Linn. and their Host Nerium indicum Mill.
Publication date: Available online 1 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Renyuan Liu, Benwei Su, Feiying Huang, Mei Ru, Hui Zhang, Zhanmin Qin, Yonghua Li, Kaixin ZhuAbstractTo evaluate the effect of the host plant on the quality of Loranthaceae species as medicinal raw material, ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to identify cardiac glycosides in Nerium indicum and its parasitic plant species Taxillus chinensis and Scurrula parasitica. Samples were collected from N. indicum and these parasi...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 2, 2019 Category: Drugs & Pharmacology Source Type: research

Highly sensitive carbon-based nanohybrid sensor platform for determination of 5-hydroxytryptamine receptor agonist (Eletriptan)
Publication date: Available online 31 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): S.I. Kaya, B. Demirkan, N.K. Bakirhan, E. Kuyuldar, S. Kurbanoglu, S.A. Ozkan, F. SenAbstractHighly sensitive nanosensors such as graphene oxide/ platinum-iridium nanohybrid, carboxylic acid functionalized multiwalled carbon nanotubes (GO/Pt-Ir/MWCNT-COOH) and amine functionalized multiwalled carbon nanotubes (GO/Pt-Ir/MWCNT-NH2) modified glassy carbon electrode were developed for the determination of 5-hydroxytryptamine receptor agonist, Eletriptan. Graphene oxide/platinum-iridium nanohybrid was synthesized us...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 1, 2019 Category: Drugs & Pharmacology Source Type: research

Biological activity and stability analyses of knipholone anthrone, a phenyl anthraquinone derivative isolated from Kniphofia foliosa Hochst
Publication date: Available online 31 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ruth Feilcke, Georgette Arnouk, Boingotlo Raphane, Khumoekae Richard, Ian Tietjen, Kerstin Andrae-Marobela, Frank Erdmann, Susanne Schipper, Katja Becker, Norbert Arnold, Andrej Frolov, Norbert Reiling, Peter Imming, Serge A.T. FobofouAbstractKnipholone (1) and knipholone anthrone (2), isolated from the Ethiopian medicinal plant Kniphofia foliosa Hochst. are two phenyl anthraquinone derivatives, a compound class known for biological activity. In the present study, we describe the activity of both 1 and 2 in sev...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 1, 2019 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography-quadrupole time of flight mass spectrometric method for targeted analysis of 111 nitrogen-based compounds in weight loss and ergogenic supplements
Publication date: Available online 31 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bharathi Avula, Ji-Yeong Bae, Amar G. Chittiboyina, Yan-Hong Wang, Mei Wang, Ikhlas A. KhanAbstractThe use of supplements for weight loss and in sports as pre-workout (ergogenic) products is widespread. Many of these supplements were found to contain active components, which were not claimed on the labels. A validated liquid chromatography high-resolution mass spectrometry quadrupole time-of-flight (LC-QToF-MS) method was developed for the simultaneous analysis of 111 amine-based compounds belonging to anorecti...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 1, 2019 Category: Drugs & Pharmacology Source Type: research

Mechanistic Insight into the Oxazoline Decomposition of DFC-M, a Synthetic Intermediate of Florfenicol
Publication date: Available online 31 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rong-Sheng Yang, Xiao Wang, Weijuan Tang, Chander Celly, Huaming ShengAbstract2-(dichloromethyl)-5[4-(methylsulfonyl)-phenyl]-4-(fluoromethyl)-oxazoline (DFC-M, 1) is a key oxazoline-containing intermediate in commercial process for the synthesis of Florfenicol (3), a marketed broad spectrum veterinary antibiotic. DFC-M was not stable in solution due to the presence of oxazoline moiety, which provided further hindrance for analytical sample preparation and HPLC analysis. Hence, the mechanistic study on the in-s...
Source: Journal of Pharmaceutical and Biomedical Analysis - June 1, 2019 Category: Drugs & Pharmacology Source Type: research

Segmented scan modes and polarity-based LC-MS for pharmacokinetic interaction study between Fufang Danshen Dripping Pill and Clopidogrel Bisulfate Tablet
Publication date: Available online 30 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shuai Ji, Xian Shao, Zhen-yu Su, Lei Ji, Yu-jie Wang, Yun-su Ma, Lu Zhao, Yan Du, Meng-zhe Guo, Dao-quan TangAbstractFufang Danshen Dripping Pill (FDDP) and Clopidogrel Bisulfate Tablet (CBT) are usually combined for treatment of coronary artery diseases in clinical. To investigate the pharmacokinetic interaction between FDDP and CBT after oral administration of FDDP, CBT and their combination in rats, a novel LC-MS method with segmented scan modes (multiple reaction monitoring and selected ion monitoring) and ...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 31, 2019 Category: Drugs & Pharmacology Source Type: research

Qualitative and quantitative analysis of sesquiterpene lactones in Centipeda minima by UPLC–Orbitrap–MS & UPLC-QQQ-MS
Publication date: Available online 30 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chi-on Chan, Xiu-juan Xie, Siu-wai Wan, Gao-li Zhou, Ailsa Chui-ying Yuen, Daniel Kam-wah Mok, Si-bao ChenAbstractAn ultra-high performance liquid chromatography coupled with high-resolution Orbitrap mass spectrometry (UPLC–Orbitrap–MS) method has been used to identify sesquiterpene lactones in the methanolic extract of Centipeda minima. Fifteen sesquiterpene lactones were tentatively identified based on retention time and accurate mass of external standards or exact accurate mass searching (within ...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 31, 2019 Category: Drugs & Pharmacology Source Type: research

An LC–MS/MS spectrometry method for the simultaneous determination of Rosuvastatin and Irbesartan in rat plasma: Insight into pharmacokinetic and drug-drug interaction studies
Publication date: Available online 31 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed Saleh Elgawish, Moustafa K. Soltan, Mahmoud M. SebaiyAbstractThe synergistic vascular protective effect of statins and angiotensin receptor blockers (ARBs) is well known, however, the pharmacokinetic interaction among these classes is yet to be understood and the necessity of developing analytical methods for their determination in vivo is gradually increased. Herein, first chromatographic separation coupled tandem mass spectrometric was developed and fully validated for simultaneous measurement of rosu...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 31, 2019 Category: Drugs & Pharmacology Source Type: research

A rapid and simple UPLC method for serum vancomycin determination in pediatric patients undergoing continuous infusion or intermittent infusion of vancomycin
Publication date: Available online 29 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Min Zhang, XiaoLan Huang, Wu Yahui, Chong Shi, Pei Pei, Jian Yang, Qu Dong, Xiaodai CuiAbstractVancomycin is a glycopeptide antibiotic widely used against Gram-positive bacteria such as methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus. Several studies have found that in adults, continuous infusions of vancomycin showed earlier attainment of desired target drug concentrations and reduced nephrotoxicity relative to intermittent infusion. In the pediatric population, there is no co...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 30, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous detection of nitrosamines and other sartan-related impurities in active pharmaceutical ingredients by supercritical fluid chromatography
Publication date: Available online 29 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sebastian Schmidtsdorff, Alexander H. SchmidtAbstractSince July 2018, the pharmacological class of “sartans” has been the subject of considerable media and analytical interest, as it became known that they are contaminated with nitrosamines such as N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA) and N-nitrosodiisopropylamine (NDiPA). Previous compendial methods are not able to detect these new contaminants. Using the latest and innovative Quality-by-Design (QbD) approach, it has now been...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 30, 2019 Category: Drugs & Pharmacology Source Type: research

Application of probe electrospray ionization-tandem mass spectrometry to ultra-rapid determination of glufosinate and glyphosate in human serum
In this study, we addressed this limiting factor by developing an ultra-rapid and simple analytical technique using a combination of probe electrospray ionization (PESI) and tandem mass spectrometry (MS/MS), which gives quantitative results within 0.3 min. Herbicide standards were added to human serum that was then subjected to analysis (N = 5 per concentration). The analysis was repeated daily over eight consecutive days. The limit of detection (LOD) was 0.59 μg/mL for glufosinate and 0.20 μg/mL for glyphosate. The limit of quantitation (LOQ), i.e., the lowest point on the calibrat...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 30, 2019 Category: Drugs & Pharmacology Source Type: research

Enhancing counterfeit and illicit medicines grouping via feature selection and X-ray fluorescence (XRF) spectrometry
Publication date: Available online 30 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Felipe Soares, Michel J. Anzanello, Flavio S. Fogliatto, Rafael S. Ortiz, Kristiane C. Mariotti, Marco F. FerrãoAbstractIn this paper, we propose a novel framework to select the most relevant X-Ray Fluorescence (XRF) energy values (i.e., features) to enhance the clustering (grouping) of counterfeit and illicit medical tablets. The framework is based on the integration of multidimensional scaling (MDS) and Procrustes analysis (PA) multivariate techniques. MDS provides a projection of the original data int...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 30, 2019 Category: Drugs & Pharmacology Source Type: research

Validation of an LC-MS/MS method for the quantitative analysis of 1P-LSD and its tentative metabolite LSD in fortified urine and serum samples including stability tests for 1P-LSD under different storage conditions
Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Christina Grumann, Kerstin Henkel, Alexander Stratford, Maren Hermanns-Clausen, Torsten Passie, Simon D. Brandt, Volker AuwärterAbstractA variety of hallucinogens of the lysergamide type has emerged on the drug market in recent years and one such uncontrolled derivative of lysergic acid diethylamide (LSD) is 1-propionyl-LSD (1PLSD). Due to the high potency of LSD and some of its derivatives (common doses: 50-200 µg), sensitive methods are required for the analysis of biological samples such as...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Evaluation of molecularly imprinted polymers for chlorpromazine and bromopromazine prepared by multi-step swelling and polymerization method. The application for the determination of chlorpromazine and its metabolites in rat plasma by column-switching LC
Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kanae Nishimura, Noboru Okamura, Tetsutaro Kimachi, Jun HaginakaAbstractMonodisperse molecularly imprinted polymers (MIPs) for chlorpromazine (CPZ) and bromopromazine (BPZ), MIPCPZ and MIPBPZ, were prepared using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a crosslinker by multi-step swelling and polymerization. The retention and molecular-recognition properties of MIPCPZ and MIPBPZ were evaluated using a mixture of potassium phosphate buffer and acetonitrile or a mixture of w...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a fast and sensitive UHPLC-DAD assay for the quantification of nitrofurantoin in plasma and urine
Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rixt A. Wijma, Karin E.J. Hoogtanders, Sander Croes, Johan W. Mouton, Roger J.M. BrüggemannAbstractNitrofurantoin is an antimicrobial drug that has been used in the treatment of lower urinary tract infections for more than 50 years. Despite its long use, surprisingly little is known of the pharmacokinetics of nitrofurantoin, whereas this is essential to optimize patient treatment. We developed a novel analytical method for the quantification of nitrofurantoin in plasma and urine using ultra-high performanc...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of bendamustine and γ-hydroxybendamustine in mice dried blood spots and its application in a mice pharmacokinetic study
Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Neeraj Kumar Saini, Suresh P Sulochana, Vinay Kiran, Sadanand Rangnathrao Mallurwar, Wolfgang Ritcher, Nuggehally R Srinivas, Ramesh MullangiAbstractA selective, sensitive and rapid mice dried blood spot (DBS) method has been developed and validated for the simultaneous quantification of bendamustine (BM) and γ-hydroxy-bendamustine (HBM) as per regulatory guidelines using an LC-MS/MS. Quality control, calibration curve and study sample DBS cards were sonicated with 5% formic acid in water before extractio...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

A New Titania Glyco-purification Tip for the Fast Enrichment and Efficient Analysis of Glycopeptides and Glycans by MALDI-TOF-MS
Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): KAYILI Hacı Mehmet, AVCI İzzet, SALİH BekirAbstractEnrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis. Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans. The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-gl...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Tailor-designed deep eutectic liquids as a sustainable extraction media: An alternative to ionic liquids
Publication date: Available online 27 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Selin ŞahinAbstractOne of the main issues of Green Chemistry, encouraging innovative technologies, where incorporates solutions to reduce or eliminate existing pollution problems, is the development of novel green solvents. Therefore, design of environmentally friendly solvents has had a strategic preface in the framework of green technologies recently. When looking at the last 20 years, it is seen that Ionic Liquids (IL) has attracted attention as new generation green design solvents. However, IL has disadvan...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Sodium, potassium, calcium lactobionates, and lactobionic acid from Zymomonas mobilis: a novel approach about stability and stress tests
This report brings an amount of results as contribution to the scarce information regarding the chemical and physical-chemical stability of sodium, potassium, calcium lactobionate, and lactobionic acid. These data may be useful and serve as reference, in view of the multipurpose applications of the cited compounds.Graphical Abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - May 29, 2019 Category: Drugs & Pharmacology Source Type: research

Structural Elucidation of a Dimeric Impurity in the Process Development of Ceftolozane using LC/HRMS and 2D-NMR
Publication date: Available online 26 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jing Liao, Huaming Sheng, Josep Saurí, Rong Xiang, Gary MartinAbstractCeftolozane is MSD’s 5th generation cephalosporin antibiotic with broad-spectrum gram-negative activity. While refining the synthetic route to the drug substance, a dimeric impurity was observed in the final active pharmaceutical ingredient (API) of ceftolozane. Through impurity enrichment and preparative isolation, the structure of the impurity was subsequently established through LC/HRMS, HRMSMS, H/D exchange and 2D-NMR studies...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 27, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of epoxide impurity in sarpogrelate hydrochloride intermediate by UHPLC and column-switching liquid chromatography
In this study, we resolved the problem by using the most common UV method with two ideas: one was to improve the absolute sensitivity, and the other was to reduce matrix effects. Both ultra high-performance liquid chromatography (UHPLC with high sensitivity LightPipe™ flow cells) and column-switching liquid chromatography methods were developed and validated for the quantitative determination of epoxide impurity in sarpogrelate hydrochloride intermediates. The limits of detection (LODs) of the UHPLC and column-switching liquid chromatography methods were 0.09 ppm (0.09 μg/g) and 0.33 ppm (0.33 μg/g), and the re...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 26, 2019 Category: Drugs & Pharmacology Source Type: research

UHPLC-HRMS study of Anti-Alzheimer’s drug candidates: metabolism of 7-MEOTA-tryptophan hybrids hampers their passage into brain
Publication date: Available online 24 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): M. Mžik, J. Karasová-Žďárová, K. Chalupová, J Korábečný, V. Palička, V. ŠestákAbstractBeing among the top five causes of death in the developed world, Alzheimer’s disease represents a major socio-economic issue. We administered a single intramuscular dose of two new hybrid anti-Alzheimer’s compounds, with 7-methoxytacrine (7-MEOTA; acetylcholinesterase inhibitor) and tryptophan (inhibitor of amyloid accumulation) in their structure, to rat...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 26, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of Artemisia annua extracts and related products by high performance liquid chromatography-tandem mass spectrometry coupled to sample treatment miniaturisation
Publication date: Available online 23 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Michele Protti, Roberto Mandrioli, Manuela Mandrone, Concettina Cappadone, Giovanna Farruggia, Ilaria Chiocchio, Emil Malucelli, Gloria Isani, Ferruccio Poli, Laura MercoliniABSTRACTArtemisinin, the main antimalarial compound of Artemisia annua L., is currently attracting increasing interest for its antiproliferative properties, but its content is highly variable, depending on several genetic, environmental and processing conditions. Aim of the present study is to analyse the artemisinin content in different pl...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 25, 2019 Category: Drugs & Pharmacology Source Type: research

Size-tunable Au@Ag nanoparticles for colorimetric and SERS dual-mode sensing of palmatine in traditional Chinese medicine
Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yaohui Gao, Ziwei Hu, Jiamin Wu, Zan Ning, Jingyi Jian, Ting Zhao, Xiaochen Liang, Xingjie Yang, Zi Yang, Qinyu Zhao, Jianping Wang, Zhigang Wang, Nicoleta Elena Dina, Ana Maria Raluca Gherman, Zhengjin Jiang, Haibo ZhouAbstractPalmatine is a protoberberine alkaloid separated from several plants and application as an anti-inflammatory and antibacterial agent in the therapy of gastrointestinal and genitourinary disorder. Thus, the fast quantification of palmatine is important in clinic medical assays. Herein, we...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 23, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a UPLC-MS/MS method for quantification of doxofylline and its metabolites in human plasma
Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Li-Na Fang, Ming-Qing Mao, Xiao-Hua Zhao, Ling Yang, Hui Jia, Shu-Yue XiaAbstractA sensitive and specific ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for simultaneous determination of doxofylline and its two metabolites in human plasma. After protein precipitation with methanol, the chromatographic separation was carried out on an ACQUITY UPLC HSS T3 column, with acetonitrile and 0.1% formic acid in water as mobile phase at a flow rate of 0.30&...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 23, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of multiple compounds of Da-Huang-Xiao-Shi decoction in rat plasma by LC-MS/MS and its application in a pharmacokinetic study
In this study, we developed a rapid, sensitive, and accurate LC-MS/MS method to simultaneously determine multiple, potentially bioactive compounds of DHXSD, including five alkaloids (berberine, phellodendrine, palmatine, jatrorrhizine, and magnoflorine), five anthraquinones (rhein, aloe-emodin, emodin, chrysophanol, and physcion), two iridoid glycosides (geniposide and genipin 1-gentiobioside), and one iridoid aglycone (genipin) in rat plasma. Plasma samples collected from rats were treated immediately with 5% acetic acid to avoid the degradation of genipin. After protein precipitation with acetonitrile containing 5% aceti...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 23, 2019 Category: Drugs & Pharmacology Source Type: research

Tissue distribution study of Angelica dahurica cv. Yubaizhi in rat by ultra–performance liquid chromatography with tandem mass spectrometry
Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): You-Bo Zhang, Gai-Gai Deng, Tian-Xia Wang, Lu Liu, Xiu-Wei YangAbstractA sensitive and specific ultra–performance liquid chromatographic–tandem mass (UPLC–MS/MS) spectrometric method was established to investigate tissue distribution of fourteen coumarins of Angelica dahurica cv. Yubaizhi roots (ADYR) in rat tissues, including isoimperatorin (1), imperatorin (2), isooxypeucedanin (3), byakangelicin (4), oxypeucedanin hydrate (5), bergapten (6), 2''R–neobyakangelicol (7), phellopterin (8)...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 23, 2019 Category: Drugs & Pharmacology Source Type: research

An integrated strategy based on characteristic fragment filter supplemented by multivariate statistical analysis in multi-stage mass spectrometry chromatograms for the large-scale detection and identification of natural plant-derived components in rat: the rhubarb case
Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yang Xu, Li Zhang, Qing Wang, Gan Luo, Xiaoyan GaoAbstractAn integrated strategy based on characteristic fragment filter (CFF) supplemented by multivariate statistical analysis (MSA) for MSn chromatograms [(CFF)s MSA] was proposed for the large-scale detection of natural plant-derived ingredients in vivo. To prove the practicability of this [(CFF)s MSA] strategy, rhubarb was taken as an example. First, representative authentic standards of homologous components contained in rhubarb were chosen, from which the f...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 23, 2019 Category: Drugs & Pharmacology Source Type: research