Nucleoside Hydrolase NH 36: A Vital Enzyme for the Leishmania Genus in the Development of T-Cell Epitope Cross-Protective Vaccines

Conclusions Nucleoside hydrolases are vital enzymes for the replication of Leishmania, conserved phylogenetic marker of the genus and strong-specific immunogens. We demonstrated that NH36 is an excellent target for chemotherapy of visceral leishmaniasis. Searching for the most immunogenic fraction of promastigotes of Leishmania we described the FML antigen of L. (L.) donovani, that has as its main component, the NH36 Nucleoside hydrolase. We developed second–generation vaccines with the FML and the NH36 native antigens, and with the NH36 recombinant protein. In addition, we obtained a third generation vaccine based upon the NH36 gene. All these vaccines were protective and curative when assayed in the murine and canine models of leishmaniasis, and the FML-vaccine called Leishmune® reduced the incidence of human and canine disease in the endemic areas. Recently we initiate the search for the most immunogenic part of the NH36 sequence aiming to optimize the vaccine efficacy. We observed that the F3 vaccine has an important role in signaling the optimal control of visceral leishmaniasis in mice and that the F1 domain is also needed for protection against tegumentary leishmaniasis. This knowledge allowed us to develop a recombinant chimera vaccine, which optimized the vaccine efficacy. Furthermore, aiming to develop a potential T-cell epitope synthetic vaccine against both human visceral and cutaneous leishmaniasis we identified the major immunogenic regions of NH36 re...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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OBJECTIVES: To describe short- and long-term neurologic prognosis of patients with thrombotic thrombocytopenic purpura and to identify clusters associated with evolution. DESIGN: Prospective French cohort. SETTING: ICU in a reference center. PATIENTS: All consecutive patients with newly diagnosed thrombocytopenic purpura. INTERVENTION: Comprehensive clinical, biological, and radiological evaluation at admission. Neurocognitive recovery was assessed using Glasgow Outcome Scale (range 1–5, with 1 representing death and 5 representing no or minimal neurologic deficit). MEASUREMENTS AND...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Online Clinical Investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Online Clinical Investigations Source Type: research
OBJECTIVES: To determine the diagnostic and clinical utility of trio-rapid genome sequencing in critically ill infants. DESIGN: In this prospective study, samples from critically ill infants were analyzed using both proband-only clinical exome sequencing and trio-rapid genome sequencing (proband and biological parents). The study occurred between April 2019 and December 2019. SETTING: Thirteen member hospitals of the China Neonatal Genomes Project spanning 10 provinces were involved. PARTICIPANTS: Critically ill infants (n = 202), from birth up until 13 months of life were enrolled based on eligibi...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Feature Articles Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
We examined the incidence and impact of early multiple organ dysfunction syndrome on clinical, functional, and disability outcomes over the year following traumatic brain injury. DESIGN: Retrospective cohort study. SETTING: Patients enrolled in the Transforming Clinical Research and Knowledge in Traumatic Brain Injury study, an 18-center prospective cohort study of traumatic brain injury patients evaluated in participating level 1 trauma centers. SUBJECTS: Adult (age> 17 yr) patients with moderate-severe traumatic brain injury (Glasgow Coma Scale
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Neurologic Critical Care Source Type: research
AbstractEndometritis in dairy cows is a major economic problem worldwide; without advances in lifestyle management and drug treatment, it causes high morbidity and death. Micro ribonucleic acid (miRNAs) these days is seen as an important part of gene control networks. It is a class of small nucleotides 20 –25, single-stranded RNA molecules. In endometritis, the inflammatory response caused by the gram-negative bacteriaEscherichia coli (E. coli) alters the expression of miRNA which can regulate the innate immune system. This manuscript reviews (1) the interaction of miRNAs with the signaling of NF- κB and dysreg...
Source: Inflammation - Category: Allergy & Immunology Source Type: research
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Source: HealthSkills Weblog - Category: Anesthesiology Authors: Tags: Uncategorized Chronic pain Clinical reasoning pain management Research Source Type: blogs
Joy Jackson is a nurse taking care of students at Wright State University. She's also pursuing her own bachelor’s degree in nursing as a student at Kettering College. Jackson is the clinical coordinator in Student Health Services at Wright State Physicians. She's also one of the Vaccine for Children coordinators for Wright State Physicians. Jackson is a 2021 Health Care Hero in the Patient Experience category. She works alongside two providers, Genessa Merritt, NP and Dr. Marc Raslich, to…
Source: bizjournals.com Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
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