Nucleoside Hydrolase NH 36: A Vital Enzyme for the Leishmania Genus in the Development of T-Cell Epitope Cross-Protective Vaccines

Conclusions Nucleoside hydrolases are vital enzymes for the replication of Leishmania, conserved phylogenetic marker of the genus and strong-specific immunogens. We demonstrated that NH36 is an excellent target for chemotherapy of visceral leishmaniasis. Searching for the most immunogenic fraction of promastigotes of Leishmania we described the FML antigen of L. (L.) donovani, that has as its main component, the NH36 Nucleoside hydrolase. We developed second–generation vaccines with the FML and the NH36 native antigens, and with the NH36 recombinant protein. In addition, we obtained a third generation vaccine based upon the NH36 gene. All these vaccines were protective and curative when assayed in the murine and canine models of leishmaniasis, and the FML-vaccine called Leishmune® reduced the incidence of human and canine disease in the endemic areas. Recently we initiate the search for the most immunogenic part of the NH36 sequence aiming to optimize the vaccine efficacy. We observed that the F3 vaccine has an important role in signaling the optimal control of visceral leishmaniasis in mice and that the F1 domain is also needed for protection against tegumentary leishmaniasis. This knowledge allowed us to develop a recombinant chimera vaccine, which optimized the vaccine efficacy. Furthermore, aiming to develop a potential T-cell epitope synthetic vaccine against both human visceral and cutaneous leishmaniasis we identified the major immunogenic regions of NH36 re...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Online Clinical Investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Online Clinical Investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Feature Articles Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
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Source: Critical Care Medicine - Category: Emergency Medicine Tags: Clinical investigations Source Type: research
We examined the incidence and impact of early multiple organ dysfunction syndrome on clinical, functional, and disability outcomes over the year following traumatic brain injury. DESIGN: Retrospective cohort study. SETTING: Patients enrolled in the Transforming Clinical Research and Knowledge in Traumatic Brain Injury study, an 18-center prospective cohort study of traumatic brain injury patients evaluated in participating level 1 trauma centers. SUBJECTS: Adult (age> 17 yr) patients with moderate-severe traumatic brain injury (Glasgow Coma Scale
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Source: Inflammation - Category: Allergy & Immunology Source Type: research
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Source: HealthSkills Weblog - Category: Anesthesiology Authors: Tags: Uncategorized Chronic pain Clinical reasoning pain management Research Source Type: blogs
Joy Jackson is a nurse taking care of students at Wright State University. She's also pursuing her own bachelor’s degree in nursing as a student at Kettering College. Jackson is the clinical coordinator in Student Health Services at Wright State Physicians. She's also one of the Vaccine for Children coordinators for Wright State Physicians. Jackson is a 2021 Health Care Hero in the Patient Experience category. She works alongside two providers, Genessa Merritt, NP and Dr. Marc Raslich, to…
Source: Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
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