Ptr/CTL0175 Is Required for the Efficient Recovery of Chlamydia trachomatis From Stress Induced by Gamma-Interferon

In this study, we carried out a screen to identify C. trachomatis genes important for recovery from IFNγ- and/or penicillin-induced stress. We found that ptr, encoding a poorly characterized secreted protease, is required for rapid recovery from IFNγ- but not penicillin-induced stress. Materials and Methods Cell Culture, Infection and C. trachomatis Strains HeLa (CCL-2, ATCC) and Vero cells (CCL-81, ATCC) were cultured in high glucose Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FBS (Natocor) without antibiotics in 5% CO2 at 37°C in a humidified atmosphere. Cells were routinely checked for Mycoplasma contamination using PCR, as described previously (van Kuppeveld et al., 1992). Infections were done by adding a previously titered suspension of purified EBs at the specified multiplicity of infection (MOI) in culture media to cell monolayers, followed by centrifugation at 2,500 ×g, 10°C, 30 min. Then infected cells were transferred to a tissue-culture incubator for the indicated hours post infection (hpi). EB purifications were carried out as described in Saka et al. (2011). EB stocks were titered by assessing the number of Inclusion Forming Units (IFUs) per microliter as described in Nguyen and Valdivia (2012), with minor modifications. Briefly, serial dilutions of EBs were added to Vero or HeLa cell monolayers seeded onto 96-well plates. At 24–30 hpi, cells were fixed with ice-cold methanol (15 min) and stained...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 5 July 2020Source: Journal of Alloys and CompoundsAuthor(s): Teakyung Um, Stephen K. Wilke, Heeman Choe, David C. Dunand
Source: Journal of Alloys and Compounds - Category: Chemistry Source Type: research
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New J. Chem., 2020, Accepted Manuscript DOI: 10.1039/D0NJ02210A, PaperXiaolei Jiang, Peng Du, Kan Mi, Fangdong Hu, Debao Wang, Xiuwen Zheng Sodium-ion batteries (SIBs) attract extensive attention as a promising alternative to lithium-ion batteries (LIBs) owing to their abundant reserves and low cost for large-scale energy storage systems. Among the electrode... The content of this RSS Feed (c) The Royal Society of Chemistry
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New J. Chem., 2020, Advance Article DOI: 10.1039/D0NJ01796E, PaperChelliah Koventhan, Venkatachalam Vinothkumar, Shen-Ming Chen, Arumugam Sangili Schematic illustration of the electrocatalytic activity of nitrofurantoin (NFT) on ZnCo2O4 nanosheets. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
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J. Mater. Chem. A, 2020, Accepted Manuscript DOI: 10.1039/D0TA03547E, PaperLu Han, Hailong Huang, Junfeng Li, Xinlu Zhang, Zhongli Yang, Min Xu, Likun Pan With the rapid development of flexible wearable electronic devices and growing energy demands in modern society, the flexible energy storage equipments attract more and more attention. Recently, flexible Zn-ion hybrid... The content of this RSS Feed (c) The Royal Society of Chemistry
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J. Mater. Chem. A, 2020, Advance Article DOI: 10.1039/D0TA03470C, CommunicationYing Pan, Yanfang Wu, H. Alex Hsain, Ran Su, Claudio Cazorla, Dewei Chu Enhanced OER performance of Ni(Fe) hydroxide through UV/ozone treatment. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
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Dalton Trans., 2020, Accepted Manuscript DOI: 10.1039/D0DT01873B, PaperRong Guo, Jian Li, Lihong Chen, Zihuan Yu, Huiqin Yao, Keren Shi, Cuiqing Li, Shulan Ma In this work, we demonstrate the tunable luminescence of the newly synthesized SDC/OS co-intercalated Mg/Al-LDH composites (SDC is 4,4'-stilbenedicarboxylic acid; OS is 1-octanesulfonic acid sodium; LDH is MgAl-layered double hydroxide)... The content of this RSS Feed (c) The Royal Society of Chemistry
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Chem. Commun., 2020, Accepted Manuscript DOI: 10.1039/D0CC02858D, CommunicationSourav Ghosh, Zhimin Qi, Haiyan Wang, Surendra Kumar Martha, Vilas Pol The impact of ester and ether-based electrolytes on the sodium-ion reversible storage in the SbOx-GNP hybrid anode synthesised by ultrafast dry microwave superheating is investigated. Kinetic and diffusion studies further... The content of this RSS Feed (c) The Royal Society of Chemistry
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