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Condition: Osteoarthritis

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Total 352 results found since Jan 2013.

MiR-145 targeting BNIP3 reduces apoptosis of chondrocytes in osteoarthritis through Notch signaling pathway.
CONCLUSIONS: MiR-145 can reduce OA-induced chondrocyte apoptosis through targeted inhibition on BNIP3 and regulation on Notch signaling pathway. PMID: 32894532 [PubMed - as supplied by publisher]
Source: European Review for Medical and Pharmacological Sciences - September 8, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Inhibition of GPR17 with pranlukast protects against TNF- α-induced loss of type II collagen in ATDC5 cells.
In this study, we demonstrate that GPR17 is expressed in ATDC5 cells and is increased in response to TNF-α exposure. We also found that antagonism of GPR17 with pranlukast significantly inhibited oxidative stress by downregulating the intracellular level of reactive oxygen species (ROS) and increasing the activity of super oxide dismutase (SOD) against TNF-α. Interestingly, treatment with pranlukast prevented TNF-α-induced reduction of type II collagen. Additionally, knockdown of GPR17 with siRNA ameliorated TNF-α-induced loss of type II collagen, suggesting the importance of the role of GPR17 in mediating the impairme...
Source: International Immunopharmacology - August 13, 2020 Category: Allergy & Immunology Authors: Wang Z, Zhou W, Zheng G, Yang G Tags: Int Immunopharmacol Source Type: research

Long non-coding RNA PCAT-1 regulates apoptosis of chondrocytes in osteoarthritis by sponging miR-27b-3p
ConclusionsBased on the comparisons and analysis, we could conclude that lncRNA PCAT-1 regulated the apoptosis of chondrocytes through sponging miR-27b-3p in OA. PCAT-1 has potential values to act as a new therapeutic target for OA patients.
Source: Journal of Bone and Mineral Metabolism - August 7, 2020 Category: Orthopaedics Source Type: research

Metformin Mitigates Cartilage Degradation by Activating AMPK/SIRT1-Mediated Autophagy in a Mouse Osteoarthritis Model
Chondrocyte dysfunction is a key mechanism underlying osteoarthritis. Metformin has shown protective effects in many diseases. The present study aimed to investigate the effects of metformin on autophagy and apoptosis in the process of osteoarthritis. A mouse osteoarthritis model was set up by surgically destabilizing medial meniscus in the knee. Intraarticular injection of metformin or vehicle was applied in the right knee for eight weeks. Mouse articular chondrocytes were isolated and passaged for in vitro experiments. Small interfering RNA (siRNA) transfection was used to silence target genes. Western blotting, immunohi...
Source: Frontiers in Pharmacology - July 23, 2020 Category: Drugs & Pharmacology Source Type: research

Suppression of p38/HBP1 pathway alleviates hyperosmotic stress-induced senescent progression of chondrocyte senescence.
This study aims to explore the effect of p38 mitogen-activated protein kinase and its downstream target HMG-box transcription factor 1 (HBP1) in the chondrocyte (CH) senescence caused by hyperosmotic stress. Human cartilage tissue with or without osteoarthritis (OA) were collected to detect the differential expression of p38 and HBP1 by Western blot. CHs were isolated from cartilage without OA and used the hyperosmotic medium to accelerate CH senescence in vitro. A p38 inhibitor and siRNA were used to mediate the expression of p38 and HBP1. The viability of CHs was determined by cell counting kit 8 (CCK8) assay. CH-related...
Source: Journal of Biological Regulators and Homeostatic Agents - June 20, 2020 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

SIRT4 suppresses the inflammatory response and oxidative stress in osteoarthritis.
This study aimed to explore the role of SIRT4 during OA and mechanisms implicated. We extracted total protein and mRNA of the cartilage from OA patients and isolated the chondrocytes from the cartilage in different degenerated degrees for cell culture. Collagen II and SIRT4 levels of the tissues were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Western blot. Chondrocytes were transferred with SIRT4-siRNA, treated with recombinant human SIRT4 protein for 24 h, respectively. Aggrecan, collagen I, collagen II, MMP-13, IL-6, TNF-α, SOD1, SOD2, and CAT expression, and ROS levels were in...
Source: American Journal of Translational Research - June 10, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Lnc-RNA BLACAT1 regulates differentiation of bone marrow stromal stem cells by targeting miR-142-5p in osteoarthritis.
CONCLUSIONS: Lnc-RNA BLACAT1 expression was increased in inflammatory BMSCs, and knockdown of BLACAT1 promoted proliferation and osteogenic differentiation of BMSCs targeting miR-142-5p. PMID: 32271407 [PubMed - as supplied by publisher]
Source: European Review for Medical and Pharmacological Sciences - April 10, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

GAB2 inhibits chondrocyte apoptosis through PI3K-AKT signaling in osteoarthritis.
Abstract Cartilage degeneration is considered the main pathologic feature of osteoarthritis (OA). Cumulative evidence indicates that chondrocyte apoptosis is associated with cartilage degradation. However, the underlying molecular mechanism of chondrocyte apoptosis remains unclear. Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Here, we explored the role of GAB2 in the pathogenesis of osteoarthritis (OA). GAB2 expression was markedly increased in OA articular cartilage. GAB2 expression was also increased in an in vitro mod...
Source: International Journal of Clinical and Experimental Pathology - April 10, 2020 Category: Pathology Authors: Cheng Z, Sun W, Ni X, Xu H, Wang Y Tags: Int J Clin Exp Pathol Source Type: research

Effect of Gubi prescription on caveolin-1 expression and phosphoinositide 3 kinase/protein kinase B and Fas signal pathways in rats with knee osteoarthritis.
CONCLUSION: Gubi prescription suppressed the chondrocyte-related PI3K/Akt and Fas signal pathways and inhibited the overexpression of caveolin-1 in rat chondrocytes. PMID: 32242388 [PubMed - in process]
Source: Journal of Traditional Chinese Medicine - March 31, 2020 Category: Complementary Medicine Authors: He X, Zhou X, Xu L, Cao J, Wang R, Wang M, Xie G, Wang L Tags: J Tradit Chin Med Source Type: research

Pinitol suppresses TNF- α-induced chondrocyte senescence.
Pinitol suppresses TNF-α-induced chondrocyte senescence. Cytokine. 2020 Mar 19;130:155047 Authors: Lou C, Deng A, Zheng H, Sun G, Zhao H, Li A, Liu Q, Li Y, Lv Z Abstract Osteoarthritis (OA) is a highly prevalent joint disorder that is tightly correlated with age. As the body ages, cell replication and function decline until homeostasis can no longer be maintained. This process involves cellular senescence as well as replicative senescence. Telomere length, cell cycle arrest, expression of p16 and p53, and the release of senescence-associated β-galactosidase (SA-β-Gal) are all markers of cell senes...
Source: Cytokine - March 18, 2020 Category: Molecular Biology Authors: Lou C, Deng A, Zheng H, Sun G, Zhao H, Li A, Liu Q, Li Y, Lv Z Tags: Cytokine Source Type: research

Nrf2/ARE is a key pathway for curcumin-mediated protection of TMJ chondrocytes from oxidative stress and inflammation.
This study provides insight into potential therapeutic approaches for TMJ OA. PMID: 32124251 [PubMed - as supplied by publisher]
Source: Cell Stress and Chaperones - March 1, 2020 Category: Cytology Authors: Jiang C, Luo P, Li X, Liu P, Li Y, Xu J Tags: Cell Stress Chaperones Source Type: research

Implication of cell migration inducing hyaluronidase 1 (CEMIP) in hyaluronan degradation by synovial fibroblasts in patients with knee osteoarthritis.
This study examined their expression in synovial tissue and the relationship with molecular weight of HA in SF in knee osteoarthritis patients. Quantification of mRNA demonstrated that HYBID expression is significantly (5.4-fold) higher in osteoarthritic synovium than in normal control synovium, whereas TMEM2 expression level is similar between the two groups. By immunohistochemistry, HYBID was localized mainly to CD68-negative and fibroblast-specific protein 1-positive synovial lining cells and sub-lining fibroblasts in osteoarthritic synovium. The mRNA expression levels of HYBID, but not TMEM2, in osteoarthritic synovium...
Source: The American Journal of Pathology - February 17, 2020 Category: Pathology Authors: Shiozawa J, de Vega S, Cilek MZ, Yoshinaga C, Nakamura T, Kasamatsu S, Yoshida H, Kaneko H, Ishijima M, Kaneko K, Okada Y Tags: Am J Pathol Source Type: research

AMD3100 Attenuates Post-Traumatic Osteoarthritis by Maintaining Transforming Growth Factor- β1-Induced Expression of Tissue Inhibitor of Metalloproteinase-3 via the Phosphatidylinositol 3-Kinase/Akt Pathway
In conclusion, inhibition of the CXCL12a/CXCR4 signaling axis maintained TIMP-3 expression via the PI3K/Akt pathway. Our findings provide insight into the mechanism by which AMD3100 prevents OA.
Source: Frontiers in Pharmacology - January 21, 2020 Category: Drugs & Pharmacology Source Type: research

YAP/TAZ regulates the expression of proteoglycan 4 and tenascin C in superficial-zone chondrocytes.
Authors: Delve E, Co V, Regmi SC, Parreno J, Schmidt TA, Kandel RA Abstract The roles of cell division control protein 42 homologue (CDC42) and actin polymerisation in regulating the phenotype of superficial-zone chondrocytes (SZCs) have been demonstrated in vitro; however, the signalling pathway(s) downstream have yet to be fully elucidated. The study hypothesis was that Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) act downstream to regulate proteoglycan 4 (PRG4) and tenascin C (TNC). Bovine SZCs grown in monolayer were treated with ML141 (CDC42 inhibitor) or the actin...
Source: European Cells and Materials - January 11, 2020 Category: Cytology Tags: Eur Cell Mater Source Type: research

MiR-203 regulates estrogen receptor α and cartilage degradation in IL-1β-stimulated chondrocytes
ConclusionsMiR-203 is critical in the onset and progression of OA, at least in part, caused by estrogen deficiency and ER α instability in OA patients, providing a novel therapeutic target for the treatment of OA.
Source: Journal of Bone and Mineral Metabolism - December 31, 2019 Category: Orthopaedics Source Type: research