Membraneless and Membrane-bound Organelles in an Anhydrobiotic Cell Line are Protected from Desiccation-induced Damage
This study offers several exciting avenues for future studies in the animal model and Pv11 cell line that will further our understanding of anhydrobiosis and may lead to advancements in storing sensitive biologics at ambient temperatures for months or years.PMID:38608858 | DOI:10.1016/j.cstres.2024.04.002 (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Clinton J Belott Oleg A Gusev Takahiro Kikawada Michael A Menze Source Type: research
Loss of the histone chaperone UNC-85/ASF1 inhibits the epigenome-mediated longevity and modulates the activity of one-carbon metabolism
Cell Stress Chaperones. 2024 Apr 10:S1355-8145(24)00065-8. doi: 10.1016/j.cstres.2024.04.003. Online ahead of print.ABSTRACTHistone H3/H4 chaperone ASF1 is a conserved factor mediating nucleosomal assembly and disassembly, playing crucial roles in processes such as replication, transcription, and DNA repair. Nevertheless, its involvement in aging has remained unclear. Here, we utilized the model organism Caenorhabditis elegans to demonstrate that the loss of UNC-85, the homolog of ASF1, leads to a shortened lifespan in a multicellular organism. Furthermore, we show that UNC-85 is required for epigenome-mediated longevity, ...
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Bideep Shrestha Anni I Nieminen Olli Matilainen Source Type: research
Membraneless and Membrane-bound Organelles in an Anhydrobiotic Cell Line are Protected from Desiccation-induced Damage
This study offers several exciting avenues for future studies in the animal model and Pv11 cell line that will further our understanding of anhydrobiosis and may lead to advancements in storing sensitive biologics at ambient temperatures for months or years.PMID:38608858 | DOI:10.1016/j.cstres.2024.04.002 (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Clinton J Belott Oleg A Gusev Takahiro Kikawada Michael A Menze Source Type: research
Loss of the histone chaperone UNC-85/ASF1 inhibits the epigenome-mediated longevity and modulates the activity of one-carbon metabolism
Cell Stress Chaperones. 2024 Apr 10:S1355-8145(24)00065-8. doi: 10.1016/j.cstres.2024.04.003. Online ahead of print.ABSTRACTHistone H3/H4 chaperone ASF1 is a conserved factor mediating nucleosomal assembly and disassembly, playing crucial roles in processes such as replication, transcription, and DNA repair. Nevertheless, its involvement in aging has remained unclear. Here, we utilized the model organism Caenorhabditis elegans to demonstrate that the loss of UNC-85, the homolog of ASF1, leads to a shortened lifespan in a multicellular organism. Furthermore, we show that UNC-85 is required for epigenome-mediated longevity, ...
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Bideep Shrestha Anni I Nieminen Olli Matilainen Source Type: research
Loss of the histone chaperone UNC-85/ASF1 inhibits the epigenome-mediated longevity and modulates the activity of one-carbon metabolism
Cell Stress Chaperones. 2024 Apr 10:S1355-8145(24)00065-8. doi: 10.1016/j.cstres.2024.04.003. Online ahead of print.ABSTRACTHistone H3/H4 chaperone ASF1 is a conserved factor mediating nucleosomal assembly and disassembly, playing crucial roles in processes such as replication, transcription, and DNA repair. Nevertheless, its involvement in aging has remained unclear. Here, we utilized the model organism Caenorhabditis elegans to demonstrate that the loss of UNC-85, the homolog of ASF1, leads to a shortened lifespan in a multicellular organism. Furthermore, we show that UNC-85 is required for epigenome-mediated longevity, ...
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Bideep Shrestha Anni I Nieminen Olli Matilainen Source Type: research
Membraneless and Membrane-bound Organelles in an Anhydrobiotic Cell Line are Protected from Desiccation-induced Damage
This study offers several exciting avenues for future studies in the animal model and Pv11 cell line that will further our understanding of anhydrobiosis and may lead to advancements in storing sensitive biologics at ambient temperatures for months or years.PMID:38608858 | DOI:10.1016/j.cstres.2024.04.002 (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Clinton J Belott Oleg A Gusev Takahiro Kikawada Michael A Menze Source Type: research
Discovery and Validation of a Novel Inhibitor of HYPE-mediated AMPylation
Cell Stress Chaperones. 2024 Apr 8:S1355-8145(24)00063-4. doi: 10.1016/j.cstres.2024.04.001. Online ahead of print.ABSTRACTAMPylation-the covalent transfer of an AMP from ATP onto a target protein-is catalyzed by the human enzyme HYPE/FicD to regulate its substrate, the heat shock chaperone BiP. HYPE-mediated AMPylation of BiP is critical for maintaining proteostasis in the ER (endoplasmic reticulum) and mounting an UPR (unfolded protein response) in times of proteostatic imbalance. Thus, manipulating HYPE's enzymatic activity is a key therapeutic strategy towards the treatment of various protein misfolding diseases, inclu...
Source: Cell Stress and Chaperones - April 10, 2024 Category: Cytology Authors: Ali Camara Heerak Chugh Alyssa George Lukas Dolidze Kevin Ryu Katrina J Holly Daniel P Flaherty Seema Mattoo Source Type: research
Discovery and Validation of a Novel Inhibitor of HYPE-mediated AMPylation
Cell Stress Chaperones. 2024 Apr 8:S1355-8145(24)00063-4. doi: 10.1016/j.cstres.2024.04.001. Online ahead of print.ABSTRACTAMPylation-the covalent transfer of an AMP from ATP onto a target protein-is catalyzed by the human enzyme HYPE/FicD to regulate its substrate, the heat shock chaperone BiP. HYPE-mediated AMPylation of BiP is critical for maintaining proteostasis in the ER (endoplasmic reticulum) and mounting an UPR (unfolded protein response) in times of proteostatic imbalance. Thus, manipulating HYPE's enzymatic activity is a key therapeutic strategy towards the treatment of various protein misfolding diseases, inclu...
Source: Cell Stress and Chaperones - April 10, 2024 Category: Cytology Authors: Ali Camara Heerak Chugh Alyssa George Lukas Dolidze Kevin Ryu Katrina J Holly Daniel P Flaherty Seema Mattoo Source Type: research
miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes
In this study, we explored the role of miR-92a-3p in the ethanol-induced apoptosis of H9c2 cardiomyocytes and identified its target genes and signaling pathways. H9c2 cells were cultured with or without 100mM ethanol for 24h. The differential expression of miR-92a-3p was verified in H9c2 cells through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To manipulate the expression of miR-92a-3p, both a mimic and an inhibitor were transfected into H9c2 cells. An annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit and apoptosis-related antibodies were used for apoptos...
Source: Cell Stress and Chaperones - April 6, 2024 Category: Cytology Authors: Yan Meng Zhenzhen Hu Chenyi Zhang Hao Bai Zhaoping Li Xinru Guo Liyong Chen Source Type: research
miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes
In this study, we explored the role of miR-92a-3p in the ethanol-induced apoptosis of H9c2 cardiomyocytes and identified its target genes and signaling pathways. H9c2 cells were cultured with or without 100mM ethanol for 24h. The differential expression of miR-92a-3p was verified in H9c2 cells through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To manipulate the expression of miR-92a-3p, both a mimic and an inhibitor were transfected into H9c2 cells. An annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit and apoptosis-related antibodies were used for apoptos...
Source: Cell Stress and Chaperones - April 6, 2024 Category: Cytology Authors: Yan Meng Zhenzhen Hu Chenyi Zhang Hao Bai Zhaoping Li Xinru Guo Liyong Chen Source Type: research
miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes
In this study, we explored the role of miR-92a-3p in the ethanol-induced apoptosis of H9c2 cardiomyocytes and identified its target genes and signaling pathways. H9c2 cells were cultured with or without 100mM ethanol for 24h. The differential expression of miR-92a-3p was verified in H9c2 cells through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To manipulate the expression of miR-92a-3p, both a mimic and an inhibitor were transfected into H9c2 cells. An annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit and apoptosis-related antibodies were used for apoptos...
Source: Cell Stress and Chaperones - April 6, 2024 Category: Cytology Authors: Yan Meng Zhenzhen Hu Chenyi Zhang Hao Bai Zhaoping Li Xinru Guo Liyong Chen Source Type: research
miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes
In this study, we explored the role of miR-92a-3p in the ethanol-induced apoptosis of H9c2 cardiomyocytes and identified its target genes and signaling pathways. H9c2 cells were cultured with or without 100mM ethanol for 24h. The differential expression of miR-92a-3p was verified in H9c2 cells through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To manipulate the expression of miR-92a-3p, both a mimic and an inhibitor were transfected into H9c2 cells. An annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit and apoptosis-related antibodies were used for apoptos...
Source: Cell Stress and Chaperones - April 6, 2024 Category: Cytology Authors: Yan Meng Zhenzhen Hu Chenyi Zhang Hao Bai Zhaoping Li Xinru Guo Liyong Chen Source Type: research
Impact of histone deacetylase inhibition and arimoclomol on heat shock protein expression and disease biomarkers in primary culture models of familial ALS
This study compared expression of these HSPs in cultured motor neurons expressing three variants linked to familial ALS: TDP-43G348C, FUSR521G or SOD1G93A. All variants were poor inducers of Hspa1a, and reduced levels of Hspa8 mRNA and protein, indicating multiple compromises in chaperoning capacity. To promote HSP expression, cultures were treated with the putative HSP co-inducer, arimoclomol, class I histone deacetylase (HDAC) inhibitors to promote active chromatin for transcription, and the combination. Treatments had variable, often different effects on expression of Hspa1a and Hspa8, depending on the ALS variant expre...
Source: Cell Stress and Chaperones - April 3, 2024 Category: Cytology Authors: Mario Fern ández Comaduran Sandra Minotti Suleima Jacob-Tomas Javeria Rizwan Nancy Larochelle Richard Robitaille Chantelle F Sephton M Vera Josephine N Nalbantoglu Heather D Durham Source Type: research
Impact of histone deacetylase inhibition and arimoclomol on heat shock protein expression and disease biomarkers in primary culture models of familial ALS
This study compared expression of these HSPs in cultured motor neurons expressing three variants linked to familial ALS: TDP-43G348C, FUSR521G or SOD1G93A. All variants were poor inducers of Hspa1a, and reduced levels of Hspa8 mRNA and protein, indicating multiple compromises in chaperoning capacity. To promote HSP expression, cultures were treated with the putative HSP co-inducer, arimoclomol, class I histone deacetylase (HDAC) inhibitors to promote active chromatin for transcription, and the combination. Treatments had variable, often different effects on expression of Hspa1a and Hspa8, depending on the ALS variant expre...
Source: Cell Stress and Chaperones - April 3, 2024 Category: Cytology Authors: Mario Fern ández Comaduran Sandra Minotti Suleima Jacob-Tomas Javeria Rizwan Nancy Larochelle Richard Robitaille Chantelle F Sephton M Vera Josephine N Nalbantoglu Heather D Durham Source Type: research
Impact of histone deacetylase inhibition and arimoclomol on heat shock protein expression and disease biomarkers in primary culture models of familial ALS
This study compared expression of these HSPs in cultured motor neurons expressing three variants linked to familial ALS: TDP-43G348C, FUSR521G or SOD1G93A. All variants were poor inducers of Hspa1a, and reduced levels of Hspa8 mRNA and protein, indicating multiple compromises in chaperoning capacity. To promote HSP expression, cultures were treated with the putative HSP co-inducer, arimoclomol, class I histone deacetylase (HDAC) inhibitors to promote active chromatin for transcription, and the combination. Treatments had variable, often different effects on expression of Hspa1a and Hspa8, depending on the ALS variant expre...
Source: Cell Stress and Chaperones - April 3, 2024 Category: Cytology Authors: Mario Fern ández Comaduran Sandra Minotti Suleima Jacob-Tomas Javeria Rizwan Nancy Larochelle Richard Robitaille Chantelle F Sephton M Vera Josephine N Nalbantoglu Heather D Durham Source Type: research
