Deep Learning-Based Nuclear Morphometry Reveals an Independent Prognostic Factor in Mantle Cell Lymphoma
Am J Pathol. 2022 Sep 20:S0002-9440(22)00282-6. doi: 10.1016/j.ajpath.2022.08.006. Online ahead of print.ABSTRACTBlastoid/pleomorphic morphology is associated with short survival in mantle cell lymphoma (MCL), but its prognostic value is overridden by proliferation index Ki-67 in multivariate analysis. Here we developed a nuclear segmentation model using deep learning, and nuclei of tumor cells in 103 MCL cases were automatically delineated. Eight nuclear morphometric attributes, including length, width, perimeter, area, length/width ratio, circularity, irregularity, and entropy, were extracted from each nucleus. The mean,...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wen-Yu Chuang Wei-Hsiang Yu Yen-Chen Lee Qun-Yi Zhang Hung Chang Lee-Yung Shih Chi-Ju Yeh Samuel Mu-Tse Lin Shang-Hung Chang Shir-Hwa Ueng Tong-Hong Wang Chuen Hsueh Chang-Fu Kuo Shih-Sung Chuang Chao-Yuan Yeh Source Type: research

Mesangial Injury and Capillary Ballooning Precede Podocyte Damage in Nephrosclerosis
Am J Pathol. 2022 Sep 20:S0002-9440(22)00283-8. doi: 10.1016/j.ajpath.2022.08.007. Online ahead of print.ABSTRACTThe development of focal and segmental glomerulosclerosis (FSGS) in consequence of glomerular hypertension due to arterial hypertension is widely considered as a podocyte disease. However, the primary damage is encountered in the mesangium. Mesangial cells, in acute settings, disconnect from their insertions to the glomerular basement membrane (GBM) causing a ballooning of capillaries and severe changes of the folding pattern of the GBM, of the arrangement of the capillaries and thus of the architecture of the t...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wilhelm Kriz Thorsten Wiech Hermann-Josef Gr öne Source Type: research

α-Subunit tyrosine phosphorylation is required for activation of the large conductance Ca < sup > 2+ < /sup > -activated potassium channel in the rabbit sphincter of Oddi
This study provides direct evidence that tyrosine phosphorylation of BKCa α-subunits is required for [Ca2+]i to activate BKCa channels in RSOSMCs, which may be the underlying physiological and pathological mechanism regulating the activity of BKCa channels in SO cells.PMID:36150507 | DOI:10.1016/j.ajpath.2022.08.005 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Dan Feng Yan-Yan Guo Wen Wang Lin-Feng Yan Ting Sun Qing-Qing Liu Guang-Bin Cui Hai-Yan Nan Source Type: research

Deep Learning-Based Nuclear Morphometry Reveals an Independent Prognostic Factor in Mantle Cell Lymphoma
Am J Pathol. 2022 Sep 20:S0002-9440(22)00282-6. doi: 10.1016/j.ajpath.2022.08.006. Online ahead of print.ABSTRACTBlastoid/pleomorphic morphology is associated with short survival in mantle cell lymphoma (MCL), but its prognostic value is overridden by proliferation index Ki-67 in multivariate analysis. Here we developed a nuclear segmentation model using deep learning, and nuclei of tumor cells in 103 MCL cases were automatically delineated. Eight nuclear morphometric attributes, including length, width, perimeter, area, length/width ratio, circularity, irregularity, and entropy, were extracted from each nucleus. The mean,...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wen-Yu Chuang Wei-Hsiang Yu Yen-Chen Lee Qun-Yi Zhang Hung Chang Lee-Yung Shih Chi-Ju Yeh Samuel Mu-Tse Lin Shang-Hung Chang Shir-Hwa Ueng Tong-Hong Wang Chuen Hsueh Chang-Fu Kuo Shih-Sung Chuang Chao-Yuan Yeh Source Type: research

Mesangial Injury and Capillary Ballooning Precede Podocyte Damage in Nephrosclerosis
Am J Pathol. 2022 Sep 20:S0002-9440(22)00283-8. doi: 10.1016/j.ajpath.2022.08.007. Online ahead of print.ABSTRACTThe development of focal and segmental glomerulosclerosis (FSGS) in consequence of glomerular hypertension due to arterial hypertension is widely considered as a podocyte disease. However, the primary damage is encountered in the mesangium. Mesangial cells, in acute settings, disconnect from their insertions to the glomerular basement membrane (GBM) causing a ballooning of capillaries and severe changes of the folding pattern of the GBM, of the arrangement of the capillaries and thus of the architecture of the t...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wilhelm Kriz Thorsten Wiech Hermann-Josef Gr öne Source Type: research

α-Subunit tyrosine phosphorylation is required for activation of the large conductance Ca < sup > 2+ < /sup > -activated potassium channel in the rabbit sphincter of Oddi
This study provides direct evidence that tyrosine phosphorylation of BKCa α-subunits is required for [Ca2+]i to activate BKCa channels in RSOSMCs, which may be the underlying physiological and pathological mechanism regulating the activity of BKCa channels in SO cells.PMID:36150507 | DOI:10.1016/j.ajpath.2022.08.005 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Dan Feng Yan-Yan Guo Wen Wang Lin-Feng Yan Ting Sun Qing-Qing Liu Guang-Bin Cui Hai-Yan Nan Source Type: research

Deep Learning-Based Nuclear Morphometry Reveals an Independent Prognostic Factor in Mantle Cell Lymphoma
Am J Pathol. 2022 Sep 20:S0002-9440(22)00282-6. doi: 10.1016/j.ajpath.2022.08.006. Online ahead of print.ABSTRACTBlastoid/pleomorphic morphology is associated with short survival in mantle cell lymphoma (MCL), but its prognostic value is overridden by proliferation index Ki-67 in multivariate analysis. Here we developed a nuclear segmentation model using deep learning, and nuclei of tumor cells in 103 MCL cases were automatically delineated. Eight nuclear morphometric attributes, including length, width, perimeter, area, length/width ratio, circularity, irregularity, and entropy, were extracted from each nucleus. The mean,...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wen-Yu Chuang Wei-Hsiang Yu Yen-Chen Lee Qun-Yi Zhang Hung Chang Lee-Yung Shih Chi-Ju Yeh Samuel Mu-Tse Lin Shang-Hung Chang Shir-Hwa Ueng Tong-Hong Wang Chuen Hsueh Chang-Fu Kuo Shih-Sung Chuang Chao-Yuan Yeh Source Type: research

Mesangial Injury and Capillary Ballooning Precede Podocyte Damage in Nephrosclerosis
Am J Pathol. 2022 Sep 20:S0002-9440(22)00283-8. doi: 10.1016/j.ajpath.2022.08.007. Online ahead of print.ABSTRACTThe development of focal and segmental glomerulosclerosis (FSGS) in consequence of glomerular hypertension due to arterial hypertension is widely considered as a podocyte disease. However, the primary damage is encountered in the mesangium. Mesangial cells, in acute settings, disconnect from their insertions to the glomerular basement membrane (GBM) causing a ballooning of capillaries and severe changes of the folding pattern of the GBM, of the arrangement of the capillaries and thus of the architecture of the t...
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Wilhelm Kriz Thorsten Wiech Hermann-Josef Gr öne Source Type: research

α-Subunit tyrosine phosphorylation is required for activation of the large conductance Ca < sup > 2+ < /sup > -activated potassium channel in the rabbit sphincter of Oddi
This study provides direct evidence that tyrosine phosphorylation of BKCa α-subunits is required for [Ca2+]i to activate BKCa channels in RSOSMCs, which may be the underlying physiological and pathological mechanism regulating the activity of BKCa channels in SO cells.PMID:36150507 | DOI:10.1016/j.ajpath.2022.08.005 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - September 23, 2022 Category: Pathology Authors: Dan Feng Yan-Yan Guo Wen Wang Lin-Feng Yan Ting Sun Qing-Qing Liu Guang-Bin Cui Hai-Yan Nan Source Type: research

Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing
Am J Pathol. 2022 Sep 9:S0002-9440(22)00202-4. doi: 10.1016/j.ajpath.2022.06.013. Online ahead of print.ABSTRACTMammographic density is associated with increased breast cancer risk. Conventional visual assessment of murine mouse models does not include quantified total density analysis. A bifurcated method was sufficient to obtain relative density scores on a broad range of two-dimensional whole mount images that contained both normal and abnormal findings. Image processing techniques, including a ridge operator and a gaussian denoising method, were used to isolate background away from mammary epithelium and use mean pixel...
Source: The American Journal of Pathology - September 17, 2022 Category: Pathology Authors: Brendan L Rooney Brian P Rooney Vinona Muralidaran Weisheng Wang Priscilla A Furth Source Type: research

Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing
Am J Pathol. 2022 Sep 9:S0002-9440(22)00202-4. doi: 10.1016/j.ajpath.2022.06.013. Online ahead of print.ABSTRACTMammographic density is associated with increased breast cancer risk. Conventional visual assessment of murine mouse models does not include quantified total density analysis. A bifurcated method was sufficient to obtain relative density scores on a broad range of two-dimensional whole mount images that contained both normal and abnormal findings. Image processing techniques, including a ridge operator and a gaussian denoising method, were used to isolate background away from mammary epithelium and use mean pixel...
Source: The American Journal of Pathology - September 17, 2022 Category: Pathology Authors: Brendan L Rooney Brian P Rooney Vinona Muralidaran Weisheng Wang Priscilla A Furth Source Type: research

Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing
Am J Pathol. 2022 Sep 9:S0002-9440(22)00202-4. doi: 10.1016/j.ajpath.2022.06.013. Online ahead of print.ABSTRACTMammographic density is associated with increased breast cancer risk. Conventional visual assessment of murine mouse models does not include quantified total density analysis. A bifurcated method was sufficient to obtain relative density scores on a broad range of two-dimensional whole mount images that contained both normal and abnormal findings. Image processing techniques, including a ridge operator and a gaussian denoising method, were used to isolate background away from mammary epithelium and use mean pixel...
Source: The American Journal of Pathology - September 17, 2022 Category: Pathology Authors: Brendan L Rooney Brian P Rooney Vinona Muralidaran Weisheng Wang Priscilla A Furth Source Type: research

Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing
Am J Pathol. 2022 Sep 9:S0002-9440(22)00202-4. doi: 10.1016/j.ajpath.2022.06.013. Online ahead of print.ABSTRACTMammographic density is associated with increased breast cancer risk. Conventional visual assessment of murine mouse models does not include quantified total density analysis. A bifurcated method was sufficient to obtain relative density scores on a broad range of two-dimensional whole mount images that contained both normal and abnormal findings. Image processing techniques, including a ridge operator and a gaussian denoising method, were used to isolate background away from mammary epithelium and use mean pixel...
Source: The American Journal of Pathology - September 17, 2022 Category: Pathology Authors: Brendan L Rooney Brian P Rooney Vinona Muralidaran Weisheng Wang Priscilla A Furth Source Type: research

Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing
Am J Pathol. 2022 Sep 9:S0002-9440(22)00202-4. doi: 10.1016/j.ajpath.2022.06.013. Online ahead of print.ABSTRACTMammographic density is associated with increased breast cancer risk. Conventional visual assessment of murine mouse models does not include quantified total density analysis. A bifurcated method was sufficient to obtain relative density scores on a broad range of two-dimensional whole mount images that contained both normal and abnormal findings. Image processing techniques, including a ridge operator and a gaussian denoising method, were used to isolate background away from mammary epithelium and use mean pixel...
Source: The American Journal of Pathology - September 17, 2022 Category: Pathology Authors: Brendan L Rooney Brian P Rooney Vinona Muralidaran Weisheng Wang Priscilla A Furth Source Type: research

Dystrophin Restoration after Adeno-Associated Virus U7-Mediated Dmd Exon Skipping Is Modulated by Muscular Exercise in the Severe D2-Mdx Duchenne Muscular Dystrophy Murine Model
In conclusion, voluntary running did not induce muscle damage and had no drastic detrimental effect on the AAV gene therapy exon skipping approach in a severe murine DMD model. Moreover, our results suggested that exercise should be an additional element to consider in the design and conception of future therapeutic approaches for DMD.PMID:36113555 | DOI:10.1016/j.ajpath.2022.07.016 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - September 16, 2022 Category: Pathology Authors: Alexandra Monceau Dylan Moutachi M égane Lemaitre Luis Garcia Capucine Trollet Denis Furling Arnaud Klein Arnaud Ferry Source Type: research