Genetic deletion of Vegfr2 in endothelial cells leads to immediate disruption of tumor vessels and aggravation of hypoxia
Am J Pathol. 2021 Nov 30:S0002-9440(21)00511-3. doi: 10.1016/j.ajpath.2021.11.003. Online ahead of print.ABSTRACTVascular endothelial growth factor (VEGF) blockers are used widely in clinics to target various types of human cancer. Although VEGF blockers exert marked tumor suppressive effects, the therapeutic effects can be limited. Moreover, accumulating evidence shows that VEGF acts not just on endothelial cells but also on various non-endothelial cells, including tumor and immune cells, suggesting a need to revisit the bona fide action of VEGF on endothelial cells using specific genetic mouse models. Here, tamoxifen-ind...
Source: The American Journal of Pathology - December 3, 2021 Category: Pathology Authors: Yasuaki Kido Tomofumi Ando Takahito Iga Masatsugu Ema Yoshiaki Kubota Ikue Tai-Nagara Source Type: research

Single cell transcriptomics reveal disrupted kidney filter cell-cell interactions after early and selective podocyte injury
Am J Pathol. 2021 Nov 30:S0002-9440(21)00513-7. doi: 10.1016/j.ajpath.2021.11.004. Online ahead of print.ABSTRACTThe health of the kidney filtration barrier requires communication between podocytes, endothelial cells and mesangial cells. Disruption of these cell-cell interactions is thought to contribute to disease progression in chronic kidney diseases (CKD). We recently demonstrated that podocyte ablation via doxycycline-inducible deletion of an essential endogenous molecule, CTCF (iCTCFpod-/-), is sufficient to drive progressive CKD. However, the earliest events connecting podocyte injury to disrupted intercellular comm...
Source: The American Journal of Pathology - December 3, 2021 Category: Pathology Authors: Abbe R Clark Jamie Marshall Yiming Zhou Monica S Montesinos Haiqi Chen Lan Nguyen Fei Chen Anna Greka Source Type: research

Genetic deletion of Vegfr2 in endothelial cells leads to immediate disruption of tumor vessels and aggravation of hypoxia
Am J Pathol. 2021 Nov 30:S0002-9440(21)00511-3. doi: 10.1016/j.ajpath.2021.11.003. Online ahead of print.ABSTRACTVascular endothelial growth factor (VEGF) blockers are used widely in clinics to target various types of human cancer. Although VEGF blockers exert marked tumor suppressive effects, the therapeutic effects can be limited. Moreover, accumulating evidence shows that VEGF acts not just on endothelial cells but also on various non-endothelial cells, including tumor and immune cells, suggesting a need to revisit the bona fide action of VEGF on endothelial cells using specific genetic mouse models. Here, tamoxifen-ind...
Source: The American Journal of Pathology - December 3, 2021 Category: Pathology Authors: Yasuaki Kido Tomofumi Ando Takahito Iga Masatsugu Ema Yoshiaki Kubota Ikue Tai-Nagara Source Type: research

Single cell transcriptomics reveal disrupted kidney filter cell-cell interactions after early and selective podocyte injury
Am J Pathol. 2021 Nov 30:S0002-9440(21)00513-7. doi: 10.1016/j.ajpath.2021.11.004. Online ahead of print.ABSTRACTThe health of the kidney filtration barrier requires communication between podocytes, endothelial cells and mesangial cells. Disruption of these cell-cell interactions is thought to contribute to disease progression in chronic kidney diseases (CKD). We recently demonstrated that podocyte ablation via doxycycline-inducible deletion of an essential endogenous molecule, CTCF (iCTCFpod-/-), is sufficient to drive progressive CKD. However, the earliest events connecting podocyte injury to disrupted intercellular comm...
Source: The American Journal of Pathology - December 3, 2021 Category: Pathology Authors: Abbe R Clark Jamie Marshall Yiming Zhou Monica S Montesinos Haiqi Chen Lan Nguyen Fei Chen Anna Greka Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

Osteochondroma Pathogenesis: Mouse Models and Mechanistic Insights into Interactions with Retinoid Signaling
Am J Pathol. 2021 Dec;191(12):2042-2051. doi: 10.1016/j.ajpath.2021.08.003.ABSTRACTOsteochondromas are cartilage-capped tumors that arise near growing physes and are the most common benign bone tumor in children. Osteochondromas can lead to skeletal deformity, pain, loss of motion, and neurovascular compression. Currently, surgery is the only available treatment for symptomatic osteochondromas. Osteochondroma mouse models have been developed to understand the pathology and the origin of osteochondromas and develop therapeutic drugs. Several cartilage regulatory pathways have been implicated in the development of osteochond...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Sonia Arely Garcia Vincent Y Ng Masahiro Iwamoto Motomi Enomoto-Iwamoto Source Type: research

Down-Regulation of Casein Kinase 1 α Contributes to Endometriosis through Phosphatase and Tensin Homolog/Autophagy-Related 7-Mediated Autophagy
Am J Pathol. 2021 Dec;191(12):2195-2202. doi: 10.1016/j.ajpath.2021.08.015.ABSTRACTThe present study aimed to explore the roles of casein kinase 1α (CK1α) in endometriosis and its underlying mechanisms. Endometrial specimen were collected from the patients and healthy volunteers. The expression patterns of CK1α, phosphatase and tensin homolog (PTEN), and autophagy-related proteins were determined using immunohistochemistry staining, Western blot analysis, and quantitative RT-PCR. Besides, the CK1α-overexpressing cells and PTEN knockdown cells were constructed in the endometrial stromal cells isolate...
Source: The American Journal of Pathology - November 23, 2021 Category: Pathology Authors: Aixiu Zhou Qiongyi Cai Yiting Hong Yuchun Lv Source Type: research

This Month in AJP
Am J Pathol. 2021 Nov 13:S0002-9440(21)00483-1. doi: 10.1016/j.ajpath.2021.11.002. Online ahead of print.NO ABSTRACTPMID:34785187 | DOI:10.1016/j.ajpath.2021.11.002 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 17, 2021 Category: Pathology Source Type: research

This Month in AJP
Am J Pathol. 2021 Nov 13:S0002-9440(21)00483-1. doi: 10.1016/j.ajpath.2021.11.002. Online ahead of print.NO ABSTRACTPMID:34785187 | DOI:10.1016/j.ajpath.2021.11.002 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 17, 2021 Category: Pathology Source Type: research

This Month in AJP
Am J Pathol. 2021 Nov 13:S0002-9440(21)00483-1. doi: 10.1016/j.ajpath.2021.11.002. Online ahead of print.NO ABSTRACTPMID:34785187 | DOI:10.1016/j.ajpath.2021.11.002 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 17, 2021 Category: Pathology Source Type: research

This Month in AJP
Am J Pathol. 2021 Nov 13:S0002-9440(21)00483-1. doi: 10.1016/j.ajpath.2021.11.002. Online ahead of print.NO ABSTRACTPMID:34785187 | DOI:10.1016/j.ajpath.2021.11.002 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 17, 2021 Category: Pathology Authors: Chhavi Chauhan Source Type: research

This Month in AJP
Am J Pathol. 2021 Nov 13:S0002-9440(21)00483-1. doi: 10.1016/j.ajpath.2021.11.002. Online ahead of print.NO ABSTRACTPMID:34785187 | DOI:10.1016/j.ajpath.2021.11.002 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 17, 2021 Category: Pathology Authors: Chhavi Chauhan Source Type: research

Slide over: advances in slide-free optical microscopy as drivers of diagnostic pathology
Am J Pathol. 2021 Nov 10:S0002-9440(21)00471-5. doi: 10.1016/j.ajpath.2021.10.010. Online ahead of print.ABSTRACTConventional clinical histopathology analysis is based on bright-field microscopy of thinly sliced tissue specimens. Although bright-field microscopy is a simple and robust method for examining microscope slides, preparing the slides needed is a lengthy and labor-intensive process. Slide-free histopathology technologies address this problem by directly imaging intact, minimally processed tissue samples using advanced optical imaging systems, bypassing the extended workflow now required for preparation of tissue ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Yehe Liu Richard M Levenson Michael W Jenkins Source Type: research

Discriminating Neoplastic from Non-Neoplastic Tissues using a microRNA-based Deep Cancer Classifier
Am J Pathol. 2021 Nov 10:S0002-9440(21)00479-X. doi: 10.1016/j.ajpath.2021.10.012. Online ahead of print.ABSTRACTNext-generation sequencing has enabled the collection of large biological datasets, allowing novel molecular-based classification methods to be developed for increased understanding of disease. microRNAs (miRNAs) are small regulatory RNA molecules that can be quantified using next-generation sequencing and are excellent classificatory markers. Here, we adapt a Deep Cancer Classifier (DCC) to differentiate neoplastic from non-neoplastic samples using comprehensive miRNA expression profiles from 1,031 human breast...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Emily Kaczmarek Blake Pyman Jina Nanayakkara Thomas Tuschl Kathrin Tyryshkin Neil Renwick Parvin Mousavi Source Type: research

Estrogen Inhibits Epithelial Progesterone Receptor-dependent Progestin Therapy Efficacy in a Mouse Model of Cervical Cancer
Am J Pathol. 2021 Nov 10:S0002-9440(21)00469-7. doi: 10.1016/j.ajpath.2021.10.008. Online ahead of print.ABSTRACTWhile the uterine cervix responds to the female sex hormone change, the role of progesterone in cervical cancer is poorly understood. It has been shown that medroxyprogesterone acetate (MPA) regresses cervical cancer in the transgenic mouse model expressing human papillomavirus type 16 (HPV16) E6 and E7 oncogenes. As MPA interacts most strongly with progesterone receptor (PR), we reasoned that PR would contribute to MPA-induced regression of cervical cancer. We also hypothesized that estrogen influences the ther...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Seunghan Baik Fabiola F Mehta Esra Unsal Yuri Park Sang-Hyuk Chung Source Type: research

Delta variants of SARS-CoV-2 cause significantly increased vaccine breakthrough COVID-19 cases in Houston, Texas
Am J Pathol. 2021 Nov 10:S0002-9440(21)00480-6. doi: 10.1016/j.ajpath.2021.10.019. Online ahead of print.ABSTRACTGenetic variants of SARS-CoV-2 have repeatedly altered the course of the COVID-19 pandemic. Delta variants of concern are now the focus of intense international attention because they are causing widespread COVID-19 disease globally and are associated with vaccine breakthrough cases. We sequenced the genomes of 16,965 SARS-CoV-2 from samples acquired March 15, 2021 through September 20, 2021 in the Houston Methodist hospital system. This sample represents 91% of all Methodist system COVID-19 patients during the ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Paul A Christensen Randall J Olsen S Wesley Long Sishir Subedi James J Davis Parsa Hodjat Debbie R Walley Jacob C Kinskey Matthew Ojeda Saavedra Layne Pruitt Kristina Reppond Madison N Shyer Jessica Cambric Ryan Gadd Rashi M Thakur Akanksha Batajoo Regan Source Type: research

Inhibition of VEGF-receptors 1 and 2 attenuates NK cell and innate immune responses in an experimental model for obliterative bronchiolitis
Am J Pathol. 2021 Nov 10:S0002-9440(21)00475-2. doi: 10.1016/j.ajpath.2021.10.018. Online ahead of print.ABSTRACTObliterative bronchiolitis (OB) after lung transplantation is a non-reversible life-threatening complication. We investigated the role of the main receptors for vascular endothelial growth factor (VEGF), VEGFR -1 and -2, in the development of obliterative airway disease (OAD), an experimental model for OB. The non-immunosuppressed recipients were transplanted with fully MHC-mismatched heterotopic tracheal allografts and received VEGFR-1 and -2 -specific monoclonal antibodies either alone or in combination, or ra...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Rainer Krebs Jussi M Tikkanen Alireza Raissadati Maria Hollm én Kishor Dhaygude Karl B Lemstr öm Source Type: research

The neuropeptide alpha-melanocyte-stimulating hormone is critical for corneal endothelial cell protection and graft survival after transplantation
In conclusion, α-MSH/MC1R signaling is critical for CEnC function and graft survival after corneal transplantation.PMID:34774519 | DOI:10.1016/j.ajpath.2021.10.016 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Zala Lu žnik Marzidovšek Tomas Blanco Zhongmou Sun Hamid Alemi Gustavo Ortiz Hayate Nakagawa Sunil K Chauhan Andrew W Taylor Ula V Jurkunas Jia Yin Reza Dana Source Type: research

ZFP36L1 regulates Fgf21 mRNA turnover and modulates alcoholic hepatic steatosis and inflammation in mice
Am J Pathol. 2021 Nov 11:S0002-9440(21)00478-8. doi: 10.1016/j.ajpath.2021.10.017. Online ahead of print.ABSTRACTZinc finger protein 36 like 1 (ZFP36L1) enhances the turnover of mRNAs containing AU-rich elements (AREs) in their 3'untranslated regions (3'UTR). The physiological and pathological functions of ZFP36L1 in liver, however, remain largely unknown. To investigate the role of ZFP36L1 in liver physiology and pathology, we generated liver-specific ZFP36L1-deficient (Zfp36l1flox/flox /Cre+; L1LKO) mice. Under normal conditions, the L1LKO mice and their littermate controls (Zfp36l1flox/flox/Cre-; L1FLX) appeared normal....
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Chandra S Bathula Jian Chen Rahul Kumar Perry J Blackshear Yogesh Saini Sonika Patial Source Type: research

Collagen XII regulates corneal stromal structure by modulating TGF- β activity
Am J Pathol. 2021 Nov 11:S0002-9440(21)00476-4. doi: 10.1016/j.ajpath.2021.10.014. Online ahead of print.ABSTRACTCollagen XII is a regulator of corneal stroma structure and function. The current study examined the roles of collagen XII in regulating corneal stromal transforming growth factor (TGF)-β activation and latency. Specifically, with the use of conventional collagen XII null mouse model, the roles of collagen XII in the regulation of TGF-β latency and activity in vivo were investigated. Functional quantification of latent TGF-β in stromal matrix was performed by using transformed mink lung reporter c...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Mei Sun Elena Koudouna Devon Cogswell Marcel Y Avila Manuel Koch Edgar M Espana Source Type: research

METTL3-mediated m6A Modification Links Liver Homeostasis and Pathology
Am J Pathol. 2021 Nov 11:S0002-9440(21)00473-9. doi: 10.1016/j.ajpath.2021.10.015. Online ahead of print.NO ABSTRACTPMID:34774849 | DOI:10.1016/j.ajpath.2021.10.015 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Wenbo Ma Tong Wu Source Type: research

Slide over: advances in slide-free optical microscopy as drivers of diagnostic pathology
Am J Pathol. 2021 Nov 10:S0002-9440(21)00471-5. doi: 10.1016/j.ajpath.2021.10.010. Online ahead of print.ABSTRACTConventional clinical histopathology analysis is based on bright-field microscopy of thinly sliced tissue specimens. Although bright-field microscopy is a simple and robust method for examining microscope slides, preparing the slides needed is a lengthy and labor-intensive process. Slide-free histopathology technologies address this problem by directly imaging intact, minimally processed tissue samples using advanced optical imaging systems, bypassing the extended workflow now required for preparation of tissue ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Yehe Liu Richard M Levenson Michael W Jenkins Source Type: research

Discriminating Neoplastic from Non-Neoplastic Tissues using a microRNA-based Deep Cancer Classifier
Am J Pathol. 2021 Nov 10:S0002-9440(21)00479-X. doi: 10.1016/j.ajpath.2021.10.012. Online ahead of print.ABSTRACTNext-generation sequencing has enabled the collection of large biological datasets, allowing novel molecular-based classification methods to be developed for increased understanding of disease. microRNAs (miRNAs) are small regulatory RNA molecules that can be quantified using next-generation sequencing and are excellent classificatory markers. Here, we adapt a Deep Cancer Classifier (DCC) to differentiate neoplastic from non-neoplastic samples using comprehensive miRNA expression profiles from 1,031 human breast...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Emily Kaczmarek Blake Pyman Jina Nanayakkara Thomas Tuschl Kathrin Tyryshkin Neil Renwick Parvin Mousavi Source Type: research

Estrogen Inhibits Epithelial Progesterone Receptor-dependent Progestin Therapy Efficacy in a Mouse Model of Cervical Cancer
Am J Pathol. 2021 Nov 10:S0002-9440(21)00469-7. doi: 10.1016/j.ajpath.2021.10.008. Online ahead of print.ABSTRACTWhile the uterine cervix responds to the female sex hormone change, the role of progesterone in cervical cancer is poorly understood. It has been shown that medroxyprogesterone acetate (MPA) regresses cervical cancer in the transgenic mouse model expressing human papillomavirus type 16 (HPV16) E6 and E7 oncogenes. As MPA interacts most strongly with progesterone receptor (PR), we reasoned that PR would contribute to MPA-induced regression of cervical cancer. We also hypothesized that estrogen influences the ther...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Seunghan Baik Fabiola F Mehta Esra Unsal Yuri Park Sang-Hyuk Chung Source Type: research

Delta variants of SARS-CoV-2 cause significantly increased vaccine breakthrough COVID-19 cases in Houston, Texas
Am J Pathol. 2021 Nov 10:S0002-9440(21)00480-6. doi: 10.1016/j.ajpath.2021.10.019. Online ahead of print.ABSTRACTGenetic variants of SARS-CoV-2 have repeatedly altered the course of the COVID-19 pandemic. Delta variants of concern are now the focus of intense international attention because they are causing widespread COVID-19 disease globally and are associated with vaccine breakthrough cases. We sequenced the genomes of 16,965 SARS-CoV-2 from samples acquired March 15, 2021 through September 20, 2021 in the Houston Methodist hospital system. This sample represents 91% of all Methodist system COVID-19 patients during the ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Paul A Christensen Randall J Olsen S Wesley Long Sishir Subedi James J Davis Parsa Hodjat Debbie R Walley Jacob C Kinskey Matthew Ojeda Saavedra Layne Pruitt Kristina Reppond Madison N Shyer Jessica Cambric Ryan Gadd Rashi M Thakur Akanksha Batajoo Regan Source Type: research

Inhibition of VEGF-receptors 1 and 2 attenuates NK cell and innate immune responses in an experimental model for obliterative bronchiolitis
Am J Pathol. 2021 Nov 10:S0002-9440(21)00475-2. doi: 10.1016/j.ajpath.2021.10.018. Online ahead of print.ABSTRACTObliterative bronchiolitis (OB) after lung transplantation is a non-reversible life-threatening complication. We investigated the role of the main receptors for vascular endothelial growth factor (VEGF), VEGFR -1 and -2, in the development of obliterative airway disease (OAD), an experimental model for OB. The non-immunosuppressed recipients were transplanted with fully MHC-mismatched heterotopic tracheal allografts and received VEGFR-1 and -2 -specific monoclonal antibodies either alone or in combination, or ra...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Rainer Krebs Jussi M Tikkanen Alireza Raissadati Maria Hollm én Kishor Dhaygude Karl B Lemstr öm Source Type: research

The neuropeptide alpha-melanocyte-stimulating hormone is critical for corneal endothelial cell protection and graft survival after transplantation
In conclusion, α-MSH/MC1R signaling is critical for CEnC function and graft survival after corneal transplantation.PMID:34774519 | DOI:10.1016/j.ajpath.2021.10.016 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Zala Lu žnik Marzidovšek Tomas Blanco Zhongmou Sun Hamid Alemi Gustavo Ortiz Hayate Nakagawa Sunil K Chauhan Andrew W Taylor Ula V Jurkunas Jia Yin Reza Dana Source Type: research

ZFP36L1 regulates Fgf21 mRNA turnover and modulates alcoholic hepatic steatosis and inflammation in mice
Am J Pathol. 2021 Nov 11:S0002-9440(21)00478-8. doi: 10.1016/j.ajpath.2021.10.017. Online ahead of print.ABSTRACTZinc finger protein 36 like 1 (ZFP36L1) enhances the turnover of mRNAs containing AU-rich elements (AREs) in their 3'untranslated regions (3'UTR). The physiological and pathological functions of ZFP36L1 in liver, however, remain largely unknown. To investigate the role of ZFP36L1 in liver physiology and pathology, we generated liver-specific ZFP36L1-deficient (Zfp36l1flox/flox /Cre+; L1LKO) mice. Under normal conditions, the L1LKO mice and their littermate controls (Zfp36l1flox/flox/Cre-; L1FLX) appeared normal....
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Chandra S Bathula Jian Chen Rahul Kumar Perry J Blackshear Yogesh Saini Sonika Patial Source Type: research

Collagen XII regulates corneal stromal structure by modulating TGF- β activity
Am J Pathol. 2021 Nov 11:S0002-9440(21)00476-4. doi: 10.1016/j.ajpath.2021.10.014. Online ahead of print.ABSTRACTCollagen XII is a regulator of corneal stroma structure and function. The current study examined the roles of collagen XII in regulating corneal stromal transforming growth factor (TGF)-β activation and latency. Specifically, with the use of conventional collagen XII null mouse model, the roles of collagen XII in the regulation of TGF-β latency and activity in vivo were investigated. Functional quantification of latent TGF-β in stromal matrix was performed by using transformed mink lung reporter c...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Mei Sun Elena Koudouna Devon Cogswell Marcel Y Avila Manuel Koch Edgar M Espana Source Type: research

METTL3-mediated m6A Modification Links Liver Homeostasis and Pathology
Am J Pathol. 2021 Nov 11:S0002-9440(21)00473-9. doi: 10.1016/j.ajpath.2021.10.015. Online ahead of print.NO ABSTRACTPMID:34774849 | DOI:10.1016/j.ajpath.2021.10.015 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Wenbo Ma Tong Wu Source Type: research

Slide over: advances in slide-free optical microscopy as drivers of diagnostic pathology
Am J Pathol. 2021 Nov 10:S0002-9440(21)00471-5. doi: 10.1016/j.ajpath.2021.10.010. Online ahead of print.ABSTRACTConventional clinical histopathology analysis is based on bright-field microscopy of thinly sliced tissue specimens. Although bright-field microscopy is a simple and robust method for examining microscope slides, preparing the slides needed is a lengthy and labor-intensive process. Slide-free histopathology technologies address this problem by directly imaging intact, minimally processed tissue samples using advanced optical imaging systems, bypassing the extended workflow now required for preparation of tissue ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Yehe Liu Richard M Levenson Michael W Jenkins Source Type: research

Discriminating Neoplastic from Non-Neoplastic Tissues using a microRNA-based Deep Cancer Classifier
Am J Pathol. 2021 Nov 10:S0002-9440(21)00479-X. doi: 10.1016/j.ajpath.2021.10.012. Online ahead of print.ABSTRACTNext-generation sequencing has enabled the collection of large biological datasets, allowing novel molecular-based classification methods to be developed for increased understanding of disease. microRNAs (miRNAs) are small regulatory RNA molecules that can be quantified using next-generation sequencing and are excellent classificatory markers. Here, we adapt a Deep Cancer Classifier (DCC) to differentiate neoplastic from non-neoplastic samples using comprehensive miRNA expression profiles from 1,031 human breast...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Emily Kaczmarek Blake Pyman Jina Nanayakkara Thomas Tuschl Kathrin Tyryshkin Neil Renwick Parvin Mousavi Source Type: research

Estrogen Inhibits Epithelial Progesterone Receptor-dependent Progestin Therapy Efficacy in a Mouse Model of Cervical Cancer
Am J Pathol. 2021 Nov 10:S0002-9440(21)00469-7. doi: 10.1016/j.ajpath.2021.10.008. Online ahead of print.ABSTRACTWhile the uterine cervix responds to the female sex hormone change, the role of progesterone in cervical cancer is poorly understood. It has been shown that medroxyprogesterone acetate (MPA) regresses cervical cancer in the transgenic mouse model expressing human papillomavirus type 16 (HPV16) E6 and E7 oncogenes. As MPA interacts most strongly with progesterone receptor (PR), we reasoned that PR would contribute to MPA-induced regression of cervical cancer. We also hypothesized that estrogen influences the ther...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Seunghan Baik Fabiola F Mehta Esra Unsal Yuri Park Sang-Hyuk Chung Source Type: research

Delta variants of SARS-CoV-2 cause significantly increased vaccine breakthrough COVID-19 cases in Houston, Texas
Am J Pathol. 2021 Nov 10:S0002-9440(21)00480-6. doi: 10.1016/j.ajpath.2021.10.019. Online ahead of print.ABSTRACTGenetic variants of SARS-CoV-2 have repeatedly altered the course of the COVID-19 pandemic. Delta variants of concern are now the focus of intense international attention because they are causing widespread COVID-19 disease globally and are associated with vaccine breakthrough cases. We sequenced the genomes of 16,965 SARS-CoV-2 from samples acquired March 15, 2021 through September 20, 2021 in the Houston Methodist hospital system. This sample represents 91% of all Methodist system COVID-19 patients during the ...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Paul A Christensen Randall J Olsen S Wesley Long Sishir Subedi James J Davis Parsa Hodjat Debbie R Walley Jacob C Kinskey Matthew Ojeda Saavedra Layne Pruitt Kristina Reppond Madison N Shyer Jessica Cambric Ryan Gadd Rashi M Thakur Akanksha Batajoo Regan Source Type: research

Inhibition of VEGF-receptors 1 and 2 attenuates NK cell and innate immune responses in an experimental model for obliterative bronchiolitis
Am J Pathol. 2021 Nov 10:S0002-9440(21)00475-2. doi: 10.1016/j.ajpath.2021.10.018. Online ahead of print.ABSTRACTObliterative bronchiolitis (OB) after lung transplantation is a non-reversible life-threatening complication. We investigated the role of the main receptors for vascular endothelial growth factor (VEGF), VEGFR -1 and -2, in the development of obliterative airway disease (OAD), an experimental model for OB. The non-immunosuppressed recipients were transplanted with fully MHC-mismatched heterotopic tracheal allografts and received VEGFR-1 and -2 -specific monoclonal antibodies either alone or in combination, or ra...
Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Rainer Krebs Jussi M Tikkanen Alireza Raissadati Maria Hollm én Kishor Dhaygude Karl B Lemstr öm Source Type: research