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Condition: Osteoarthritis

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Total 352 results found since Jan 2013.

Inhibition of YAP with siRNA prevents cartilage degradation and ameliorates osteoarthritis development
AbstractThe Hippo/YAP signaling pathway is important for mediating organ size and tissue homeostasis, but its role in osteoarthritis (OA) remains unclear. We aimed to investigate the role of Hippo/YAP signaling pathway in OA development. YAP expression in OA cartilage was assessed by immunohistochemistry, RT-qPCR, and Western blotting. The effects of YAP overexpression or knockdown on gene expression related to chondrocyte hypertrophy induced by IL-1 β were examined. The in vivo effects of YAP inhibition were studied. Subchondral bone was analyzed by micro-CT. YAP was increased in mice and human OA articular cartilage and...
Source: Journal of Molecular Medicine - November 21, 2018 Category: Molecular Biology Source Type: research

Intra ‐articular silencing of periostin via nanoparticle‐based siRNA ameliorates post‐traumatic osteoarthritis in mice
ConclusionIntra-articular delivery of Postn siRNA nanocomplex represents a promising clinical approach to mitigate the severity of joint degeneration and provides an unequivocal scientific rationale for longitudinal studies. Employing a cartilage-specific gene knockout strategy will further illuminate the functional role of Postn in OA.
Source: Arthritis and Rheumatology - May 13, 2021 Category: Rheumatology Authors: Xin Duan, Lei Cai, Christine T.N. Pham, Yousef Abu ‐Amer, Hua Pan, Robert H. Brophy, Samuel A. Wickline, Muhammad Farooq Rai Tags: FULL LENGTH Source Type: research

Effective knock down of matrix metalloproteinase‐13 by an intra‐articular injection of small interfering RNA (siRNA) in a murine surgically‐induced osteoarthritis model
This study investigated the effect of MMP‐13 gene knock down on cartilage degradation by injecting small interfering RNA (siRNA) into knee joints in a mouse model of osteoarthritis (OA). OA was induced in male C57BL/6 mice by destabilization of medial meniscus (DMM) surgery. Change of Mmp13 expression over time was determined by qPCR analysis from 3 days to 6 weeks after surgery. Mmp13 and control chemically modified siRNA were injected into the knee joint 1 week after surgery and expression levels were assessed in synovium by qPCR 48 h later. Cartilage degradation was histologically assessed 8 weeks after DMM surgery ...
Source: Journal of Orthopaedic Research - May 23, 2014 Category: Orthopaedics Authors: Ryuichiro Akagi, Takahisa Sasho, Masahiko Saito, Jun Endo, Satoshi Yamaguchi, Yuta Muramatsu, Shunsuke Mukoyama, Yorikazu Akatsu, Joe Katsuragi, Taisuke Fukawa, Kazuhisa Takahashi Tags: Research Article Source Type: research

Effect of inhibiting MMP13 and ADAMTS5 by intra-articular injection of small interfering RNA in a surgically induced osteoarthritis model of mice
AbstractMatrix metalloproteinase 13 (MMP13) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) are thought to play critical roles in cartilage degradation at the early phase of osteoarthritis (OA). The aim of this study is to examine the effect of chemically modifiedMmp13 orAdamts5 small interfering RNA (siRNA), alone or in combination, in a mouse OA model. OA pathology was surgically induced in 9-week-old male C57/BL6 mice (n = 64) via destabilization of the medial meniscus (DMM). We used chemically modified siRNA (Accell siRNAs®) forMmp13 andAdamts5, as well as a non-targeting control and...
Source: Cell and Tissue Research - January 23, 2017 Category: Cytology Source Type: research

Sanguinarine Induces Apoptosis Pathway in Multiple Myeloma Cell Lines via Inhibition of the JaK2/STAT3 Signaling
In this study, we aimed to investigate the potential anti-proliferative and pro-apoptotic activities of SNG in a panel of MM cell lines (U266, IM9, MM1S, and RPMI-8226). SNG treatment of MM cells resulted in a dose-dependent decrease in cell viability through mitochondrial membrane potential loss and activation of caspase 3, 9, and cleavage of PARP. Pre-treatment of MM cells with a universal caspase inhibitor, Z-VAD-FMK, prevented SNG mediated loss of cell viability, apoptosis, and caspase activation, confirming that SNG-mediated apoptosis is caspase-dependent. The SNG-mediated apoptosis appears to be resulted from suppres...
Source: Frontiers in Oncology - April 16, 2019 Category: Cancer & Oncology Source Type: research

Amelioration of Posttraumatic Osteoarthritis in Mice Using Intraarticular Silencing of Periostin via Nanoparticle ‐Based Small Interfering RNA
ConclusionIA delivery of the periostin –siRNA nanocomplex represents a promising clinical approach to mitigate the severity of joint degeneration in OA. Our findings may thus provide an unequivocal scientific rationale for longitudinal studies of this approach. Utilizing a cartilage-specific gene-knockout strategy will further illumina te the functional role of periostin in OA.
Source: Arthritis and Rheumatology - October 28, 2021 Category: Rheumatology Authors: Xin Duan, Lei Cai, Christine T. N. Pham, Yousef Abu ‐Amer, Hua Pan, Robert H. Brophy, Samuel A. Wickline, Muhammad Farooq Rai Tags: Full Length Source Type: research

Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression.
This study examined whether HMGB1 might be involved in the treatment of RA using MTX. Synovial tissues were collected from RA patients who were treated with MTX for at least 6 months (RA-MTX group, 7 cases) and from those without MTX treatment (RA-noMTX group, 7 cases). Additionally, patients with osteoarthritis (OA group, 7 cases) were used as controls. The expression and locations of HMGB1 in the tissues were detected using real-time PCR, western blot, and immunohistochemistry. Additionally, OA-fibroblast-like synoviocytes (FLSs) and RA-FLSs were isolated and cultured, and the expression of HMGB1 was reduced in these ce...
Source: Molecular and Cellular Biochemistry - August 12, 2016 Category: Biochemistry Authors: Li YB, Xu P, Xu K, Cai YS, Sun MY, Yang L, Sun J, Lu SM Tags: Mol Cell Biochem Source Type: research

Single vs. repeated matrix metalloproteinase-13 knockdown with intra-articular short interfering RNA administration in a murine osteoarthritis model.
CONCLUSIONS: Our results suggested that the duration of siRNA effect in the knee joint lasts for at least 1 week, and that no further benefit is achieved by multiple injections. PMID: 30345823 [PubMed - as supplied by publisher]
Source: Connective Tissue Research - October 23, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Lnc-RNA BLACAT1 regulates differentiation of bone marrow stromal stem cells by targeting miR-142-5p in osteoarthritis.
CONCLUSIONS: Lnc-RNA BLACAT1 expression was increased in inflammatory BMSCs, and knockdown of BLACAT1 promoted proliferation and osteogenic differentiation of BMSCs targeting miR-142-5p. PMID: 32271407 [PubMed - as supplied by publisher]
Source: European Review for Medical and Pharmacological Sciences - April 10, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MicroRNA-199-3p up-regulation enhances chondrocyte proliferation and inhibits apoptosis in knee osteoarthritis via DNMT3A repression
ConclusionThis study highlights that miR-199-3p up-regulation or down-regulation of DNMT3A induces chondrocyte proliferation and inhibits apoptosis in KOA, which may widen our eyes to treat patients with KOA.
Source: Inflammation Research - January 12, 2021 Category: Research Source Type: research

Biphasic activation of nuclear factor-kappa B in chondrocyte death induced by interleukin-1beta: The expression of inducible nitric oxide synthase and phagocyte-type NADPH oxidase through immediate and monocarboxylate transporter-1-mediated late-phase activation of nuclear factor-kappa B
Conclusion We found that MCT-1 contributed to the expression of NOX-2 via late-phase activation of nuclear factor κB in a ROS-dependent manner in cells exposed to IL-1β. Hence, MCT-1 could be a potential target for the treatment of degenerative joint diseases.
Source: Journal of Oral Biosciences - February 24, 2016 Category: Biomedical Science Source Type: research

Underlying mechanism of Sirt1 on apoptosis and extracellular matrix degradation of osteoarthritis chondrocytes.
In conclusion, upregulation of Sirt1 expression may inhibit OA chondrocyte apoptosis and extracellular matrix degradation by increasing Bcl‑2 expression and decreasing Bax, MMP1 and MMP13 expression, via downregulation of p38, JNK and ERK phosphorylation. PMID: 28586000 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - June 8, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Applications of RNA interference in the treatment of arthritis.
Abstract RNA interference (RNAi) is a cellular mechanism for post-transcriptional gene regulation mediated by small interfering RNA (siRNA) and microRNA. siRNA-based therapy holds significant promise for the treatment of a wide-range of arthritic diseases. siRNA selectively suppresses the expression of a gene product and can thus achieve the specificity that is lacking in small molecule inhibitors. The potential use of siRNA-based therapy in arthritis, however, has not progressed to clinical trials despite ample evidence for efficacy in preclinical studies. One of the main challenges to clinical translation is the...
Source: Translational Research : the journal of laboratory and clinical medicine - July 9, 2019 Category: Laboratory Medicine Authors: Rai MF, Pan H, Yan H, Sandell LJ, Pham CTN, Wickline SA Tags: Transl Res Source Type: research

LncPVT1 promotes cartilage degradation in diabetic OA mice by downregulating miR-146a and activating TGF- β/SMAD4 signaling
ConclusionsOur results demonstrate LncRNA PVT1 is involved in cartilage degradation in diabetic OA and correlated with disease severity. Efficiency of ad-siRNA-PVT1 in controlling joint inflammation in diabetic OA mice is associated with the suppression of the expression of miR-146a, pro-inflammatory cytokines and activation of TGF- β/SMAD4 pathway.
Source: Journal of Bone and Mineral Metabolism - February 10, 2021 Category: Orthopaedics Source Type: research

Monocarboxylate transporter 4, associated with the acidification of synovial fluid, is a novel therapeutic target for inflammatory arthritis
Conclusion. RA activity correlated with decreased synovial fluid pH. This may be due to increased MCT4 expression in RASFs. Silencing MCT4 induced apoptosis in RASFs and reduced the severity of CIA, suggesting that MCT4 is a potential therapeutic target for inflammatory arthritis. This article is protected by copyright. All rights reserved.
Source: Arthritis and Rheumatism - July 20, 2015 Category: Rheumatology Authors: Wataru Fujii, Yutaka Kawahito, Hidetake Nagahara, Yuji Kukida, Takahiro Seno, Aihiro Yamamoto, Masataka Kohno, Ryo Oda, Daigo Taniguchi, Hiroyoshi Fujiwara, Akika Ejima, Tsunao Kishida, Osam Mazda, Eishi Ashihara Tags: Full Length Source Type: research