Collagen receptor- and metalloproteinase-dependent hypertensive stress response in mesangial and glomerular endothelial cells.
Abstract Progressive alteration of the extracellular matrix (ECM) is the characteristic of hypertensive nephropathy (HN). Both mesangial and endothelial cells have the ability to synthesize and degrade ECM components, including collagens through the activation of matrix metalloproteinases (MMPs) in stress conditions, such as in hypertension. On the other hand, hydrogen sulfide (H2S) has been shown to mitigate hypertensive renal matrix remodeling. Surprisingly, whether H2S ameliorates receptor-mediated (urokinase plasminogen activator receptor-associated protein, uPARAP/Endo180) collagen dysregulation in Ang-II hyp...
Source: Molecular and Cellular Biochemistry - January 7, 2020 Category: Biochemistry Authors: Majumder S, Amin M, Pushpakumar S, Sen U Tags: Mol Cell Biochem Source Type: research

MicroRNA-9119 regulates cell viability of granulosa cells in polycystic ovarian syndrome via mediating Dicer expression.
In conclusion, GCs of PCOS, the expression of miR-9119, and targeted NFκB/p65-DICER axis are upregulated in order to maintain cell viability and prevent apoptosis, thereby promoting Anti-Müllerian hormone production in GCs. This study may provide a new understanding of the mechanism of GC dysfunction. PMID: 31894528 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - January 1, 2020 Category: Biochemistry Authors: Ding Y, He P, Li Z Tags: Mol Cell Biochem Source Type: research

Chemosensory bitter taste receptors T2R4 and T2R14 activation attenuates proliferation and migration of breast cancer cells.
The objective of the current study is to investigate the expression of T2R4 and T2R14 in BC clinical samples and to examine their physiological role using highly metastatic BC and non-cancerous cell lines. Using approaches, which involve receptor knockdown, pharmacological activation and biochemical assays we report that (i) T2R4 and T2R14 expression patterns are dissimilar, with decreased levels of T2R4 and increased levels of T2R14 in BC clinical samples compared to non-cancerous controls. (ii) Activation of T2Rs with their respective agonist elicited physiological responses in metastatic breast cancer cells, and no resp...
Source: Molecular and Cellular Biochemistry - January 1, 2020 Category: Biochemistry Authors: Singh N, Shaik FA, Myal Y, Chelikani P Tags: Mol Cell Biochem Source Type: research

Curcumin amends Ca2+ dysregulation in microglia by suppressing the activation of P2X7 receptor.
Abstract Curcumin (Cur) is widely used as an anti-inflammation agent and has anti-depression potential. Neuroinflammation mediated by Ca2+ channel activation is closely associated with the progression of post-stroke depression (PSD). In the current study, the role of P2X7 receptor (P2X7R) in the anti-PSD function of Cur was explored. Rats were subjected to middle cerebral artery occlusion (MCAO) surgery and chronic mild stress administration to induce PSD symptoms and then treated with Cur. The behaviors of rats were assessed with sucrose preference and forced swim tests. The accumulation of Ca2+ and the systemic ...
Source: Molecular and Cellular Biochemistry - January 1, 2020 Category: Biochemistry Authors: Wang Z, Ren W, Zhao F, Han Y, Liu C, Jia K Tags: Mol Cell Biochem Source Type: research

LncRNA MEG3 inhibits the inflammatory response of ankylosing spondylitis by targeting miR-146a.
Abstract Ankylosing spondylitis (AS) is a progressive systemic disease characterized by chronic inflammation response of the sacroiliac joint and spine. Long non-coding RNAs (lncRNAs) are widely involved in the regulation of various diseases. However, the role of lncRNA maternally expressed gene 3 (MEG3) in the inflammatory response of AS has not been studied. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in tissues and cells. The expression levels of MEG3, m...
Source: Molecular and Cellular Biochemistry - January 1, 2020 Category: Biochemistry Authors: Li Y, Zhang S, Zhang C, Wang M Tags: Mol Cell Biochem Source Type: research

Hypoxia induces the activation of hepatic stellate cells through the PVT1-miR-152-ATG14 signaling pathway.
Abstract Increasing studies have indicated that hypoxia serves as a pivotal microenvironmental factor that facilitates activation of hepatic stellate cells (HSCs). However, the mechanism by which hypoxia activates HSCs is not clear. Here, we demonstrated that plasmacytoma variant translocation 1 (PVT1) and autophagy were overexpressed in liver fibrotic specimens. In primary mouse HSCs, both PVT1 and autophagy were induced by hypoxia. Further study showed that hypoxia-induced autophagy depended on expression of PVT1 and miR-152 in HSCs. Luciferase reporter assay indicated that autophagy-related gene 14 (ATG14) was ...
Source: Molecular and Cellular Biochemistry - December 21, 2019 Category: Biochemistry Authors: Yu F, Dong B, Dong P, He Y, Zheng J, Xu P Tags: Mol Cell Biochem Source Type: research

Bone marrow mesenchymal stem cell-secreted exosomes carrying microRNA-125b protect against myocardial ischemia reperfusion injury via targeting SIRT7.
In this study, we aimed to investigate the effect of bone marrow mesenchymal stem cell (BMSC)-derived exosomes carrying miR-125b on I/R rats. The myocardial I/R model in rats was constructed by ligation of the left anterior descending coronary artery (LAD). Rats were randomly divided into I/R and Sham group. Lv-cel-miR-67 (control) or Lv-miR-125b was transfected into BMSCs. Exosomes were extracted from transfected BMSCs, and separately named BMSC-Exo-67, BMSC-Exo-125b, and BMSC-Exo. MTT assay and flow cytometry were used to detect the viability and apoptosis of I/R myocardium cells, respectively. The expression of cel...
Source: Molecular and Cellular Biochemistry - December 19, 2019 Category: Biochemistry Authors: Chen Q, Liu Y, Ding X, Li Q, Qiu F, Wang M, Shen Z, Zheng H, Fu G Tags: Mol Cell Biochem Source Type: research

LncRNA nuclear-enriched abundant transcript 1 regulates hypoxia-evoked apoptosis and autophagy via mediation of microRNA-181b.
Abstract Nuclear-enriched abundant transcript 1 (NEAT1), a vital long noncoding RNA (lncRNA), exhibits the functions in disparate cancers. Nevertheless, the influences of NEAT1 in congenital heart disease (CHD) remain unreported. The research delves into whether NEAT1 affects H9c2 cells apoptosis and autophagy under the hypoxia condition. Overexpressed NEAT1 vector was transfected into H9c2 cells; then, functions of NEAT1 in cell viability, apoptosis, autophagy, PI3K/AKT/mTOR and JAK1/STAT3 pathways were detected in H9c2 cells under hypoxia condition. Expression of NEAT1 and miR-181b in hypoxia and blood samples f...
Source: Molecular and Cellular Biochemistry - December 18, 2019 Category: Biochemistry Authors: Lv Y, Liu Z, Huang J, Yu J, Dong Y, Wang J Tags: Mol Cell Biochem Source Type: research

Substance P receptor blocker, aprepitant, inhibited cutaneous and other neurogenic inflammation side effects of the EGFR1-TKI, erlotinib.
Abstract Cutaneous changes like rash and hair loss, as well as other neurogenic inflammation side effects, occur frequently during anticancer treatment with the epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), erlotinib. These adverse events may be so severe that they impair the patient's compliance with the treatment or even cause its discontinuation. In the current preclinical study, rats (9.2 weeks) were treated with erlotinib (10 mg/kg/day) ± aprepitant (2 mg/kg/day) for 12 weeks. Visual changes in the development of facial skin lesions/hair loss a...
Source: Molecular and Cellular Biochemistry - December 18, 2019 Category: Biochemistry Authors: Chmielinska JJ, Kramer JH, Mak IT, Spurney CF, Weglicki WB Tags: Mol Cell Biochem Source Type: research

Transforming growth factor- β1 enhances proliferative and metastatic potential by up-regulating lymphoid enhancer-binding factor 1/integrin αMβ2 in human renal cell carcinoma.
Transforming growth factor-β1 enhances proliferative and metastatic potential by up-regulating lymphoid enhancer-binding factor 1/integrin αMβ2 in human renal cell carcinoma. Mol Cell Biochem. 2019 Dec 17;: Authors: Liu Y, Shang D Abstract Renal cell carcinoma (RCC) is a kind of malignant tumor with high recurrence, and it is urgent to find molecular markers for diagnosis and prognosis of RCC. Our study investigated the expression and function of integrin αMβ2 in RCC cells, aiming to understand the role of integrin αMβ2 in RCC and develop new therapeutic target fo...
Source: Molecular and Cellular Biochemistry - December 17, 2019 Category: Biochemistry Authors: Liu Y, Shang D Tags: Mol Cell Biochem Source Type: research

The impact of PSRC1 overexpression on gene and transcript expression profiling in the livers of ApoE-/- mice fed a high-fat diet.
Abstract Our previous studies have confirmed that proline/serine-rich coiled-coil 1 (PSRC1) overexpression can regulate blood lipid levels and inhibit atherosclerosis (AS) development. In the current study, the gene and transcript expression profiles in the livers of ApoE-/- mice overexpressing PSRC1 were investigated. HiSeq X Ten RNA sequencing (RNA-seq) analysis was used to examine the differentially expressed genes (DEGs) and differentially expressed transcripts in the livers of PSRC1-overexpressing ApoE-/- and control mice. Then, Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Geno...
Source: Molecular and Cellular Biochemistry - December 14, 2019 Category: Biochemistry Authors: Wei M, Li P, Guo K Tags: Mol Cell Biochem Source Type: research

The functional impact of the C/N-terminal extensions of the mouse retinal IMPDH1 isoforms: a kinetic evaluation.
Abstract Mutations in the retinal inosine monophosphate dehydrogenase1 (IMPDH1) gene is believed to be one cause of retinitis pigmentosa (RP). The main structural difference between the mutation-susceptible retinal isoforms with canonical one resides in the C- and N-terminal extensions. There are limited studies on the structure and function of terminal peptide extensions of the IMPDH1 retinal isoforms. Using recombinant murine IMPDH1 (mH1), we evaluated the kinetics of the retinal isoforms along with inhibition by some of the purine nucleotides. Molecular modeling tools were also applied to study the probable eff...
Source: Molecular and Cellular Biochemistry - December 14, 2019 Category: Biochemistry Authors: Andashti B, Yazdanparast R, Barzegari E, Galehdari H Tags: Mol Cell Biochem Source Type: research

Characterization of IRE1 α in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches.
Characterization of IRE1α in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches. Mol Cell Biochem. 2019 Dec 13;: Authors: Oh-Hashi K, Kohno H, Kandeel M, Hirata Y Abstract IRE1 is the most conserved endoplasmic reticulum (ER)-resident stress sensor. Its activation not only splices XBP1 but also participates in a variety of cell signaling. We elucidated the role of IRE1α in Neuro2a cells by establishing IRE1α-deficient cells and applying four IRE1 inhibitors. IRE1α deficiency prevented almost all spliced XBP1 (sXBP1) protein expression by treatment with thapsigargin (Tg...
Source: Molecular and Cellular Biochemistry - December 13, 2019 Category: Biochemistry Authors: Oh-Hashi K, Kohno H, Kandeel M, Hirata Y Tags: Mol Cell Biochem Source Type: research

Role of alpha-melanocyte stimulating hormone ( α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress.
This study revealed the increase in the expression of genes related to melanogenesis, cell cycle, heat shock proteins, and apoptosis of the cells after UVR stress and demonstrated the role of paracrine factor (α-MSH) in elevating the protection response to stressful conditions like UVR stress by increasing the melanogenesis and decreasing the mitochondrial-mediated apoptosis. Based on the results of the present study, it can be stated that α-MSH can play a pivotal role in the protection of animal skin cells under stressful conditions in climate-changing scenario. PMID: 31823188 [PubMed - as supplied by pub...
Source: Molecular and Cellular Biochemistry - December 10, 2019 Category: Biochemistry Authors: Choudhary R, Sharma A, Kumar S, Upadhyay RC, Singh SV, Mohanty A Tags: Mol Cell Biochem Source Type: research

Potential therapeutic effects of antagonizing adenosine A2A receptor, curcumin and niacin in rotenone-induced Parkinson's disease mice model.
In conclusion, the combination of curcumin, niacin and ZM241385 recorded the most potent treatment effect in Parkinsonism mice followed by ZM241385, as a single treatment. ZM241385 succeeded to antagonize adenosine A2A receptor by diminishing its gene expression and ameliorating all biochemical parameters under investigation. The newly investigated agent; ZM241385 has almost the same pattern of improvement as the classical drug; Sinemet®. This could shed the light to the need of detailed studies on ZM241385 for its possible role as a promising treatment against PD. Additionally, food supplements such as curcumin a...
Source: Molecular and Cellular Biochemistry - December 9, 2019 Category: Biochemistry Authors: Motawi TK, Sadik NAH, Hamed MA, Ali SA, Khalil WKB, Ahmed YR Tags: Mol Cell Biochem Source Type: research

ROS and diseases: role in metabolism and energy supply.
Abstract Researches dedicated to reactive oxygen species (ROS) had been performed for decades, yet the outcomes remain controversial. With the relentless effort of studies, researchers have explored the role of ROS in biosystem and various diseases. ROS are beneficial for biosystem presenting as signalling molecules and enhancing immunologic defence. However, they also have harmful effects such as causing tissue and organ damages. The results are controversial in studies focusing on ROS and ROS-related diseases by regulating ROS with inhibitors or promotors. These competing results hindered the process for further...
Source: Molecular and Cellular Biochemistry - December 7, 2019 Category: Biochemistry Authors: Yang S, Lian G Tags: Mol Cell Biochem Source Type: research

Ephx2-gene deletion affects acetylcholine-induced relaxation in angiotensin-II infused mice: role of nitric oxide and CYP-epoxygenases.
Abstract Previously, we showed that adenosine A2A receptor induces relaxation independent of NO in soluble epoxide hydrolase-null mice (Nayeem et al. in Am J Physiol Regul Integr Comp Physiol 304:R23-R32, 2013). Currently, we hypothesize that Ephx2-gene deletion affects acetylcholine (Ach)-induced relaxation which is independent of A2AAR but dependent on NO and CYP-epoxygenases. Ephx2-/- aortas showed a lack of sEH (97.1%, P 
Source: Molecular and Cellular Biochemistry - December 4, 2019 Category: Biochemistry Authors: Hanif A, Edin ML, Zeldin DC, Nayeem MA Tags: Mol Cell Biochem Source Type: research

Matrix regeneration proteins in the hypoxia-triggered exosomes of shoulder tenocytes and adipose-derived mesenchymal stem cells.
Abstract Regenerative functions of exosomes rely on their contents which are influenced by pathological stimuli, including hypoxia, in rotator cuff tendon injuries (RCTI). The hypoxic environment triggers tenocytes and adjacent adipose-derived mesenchymal stem cells (ADMSCs) to release regenerative mediators to the ECM via the exosomes which elicit autocrine/paracrine responses to protect the tendon matrix from injury. We investigated the exosomal protein contents from tenocytes and subcutaneous ADMSCs from the shoulder of Yucatan microswine cultured under hypoxic conditions (2% O2). The exosomal proteins were det...
Source: Molecular and Cellular Biochemistry - December 3, 2019 Category: Biochemistry Authors: Thankam FG, Chandra I, Diaz C, Dilisio MF, Fleegel J, Gross RM, Agrawal DK Tags: Mol Cell Biochem Source Type: research

LncRNA SNHG1 alleviates hypoxia-reoxygenation-induced vascular endothelial cell injury as a competing endogenous RNA through the HIF-1 α/VEGF signal pathway.
LncRNA SNHG1 alleviates hypoxia-reoxygenation-induced vascular endothelial cell injury as a competing endogenous RNA through the HIF-1α/VEGF signal pathway. Mol Cell Biochem. 2019 Dec 02;: Authors: Liang S, Ren K, Li B, Li F, Liang Z, Hu J, Xu B, Zhang A Abstract Long noncoding ribonucleic acids (lncRNAs) are critical regulators in various biological processes. In the present study, we aimed to explore whether miR140-3p was involved in the underlying molecular mechanisms of small nucleolar RNA host gene 1 (SNHG1) in myocardial ischemia/reperfusion (I/R) injury. A mouse model of I/R injury and hy...
Source: Molecular and Cellular Biochemistry - December 2, 2019 Category: Biochemistry Authors: Liang S, Ren K, Li B, Li F, Liang Z, Hu J, Xu B, Zhang A Tags: Mol Cell Biochem Source Type: research

Catestatin reverses the hypertrophic effects of norepinephrine in H9c2 cardiac myoblasts by modulating the adrenergic signaling.
This study, for the first time, provides insights into the modulation of adrenergic signaling in the heart by CST. PMID: 31792650 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - December 2, 2019 Category: Biochemistry Authors: Alam MJ, Gupta R, Mahapatra NR, Goswami SK Tags: Mol Cell Biochem Source Type: research

Role of oxidative stress-related biomarkers in heart failure: galectin 3, α1-antitrypsin and LOX-1: new therapeutic perspective?
In conclusion, α1-antitrypsin and lectin-like oxidized low-density-lipoprotein receptor-1 seem to represent two good markers in HF and therapeutic targets, whereas galectin-3 does not. PMID: 31782085 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - November 29, 2019 Category: Biochemistry Authors: Lubrano V, Balzan S Tags: Mol Cell Biochem Source Type: research

Eukaryotic initiation factor 4E is a novel effector of mTORC1 signaling pathway in cross talk with Mnk1.
Abstract Cellular signals that influence Cap-dependent translation have assumed significant relevance in the backdrop of their enforced dysregulation during oncogenesis. Eukaryotic initiation factor 4E(eIF4E), the mRNA cap-binding protein, has emerged as a key player to facilitate tumor progression through upregulated cap-dependent translation synchronized with enhanced cell division. We provide evidence that eIF4E phosphorylation is regulated by mTORC1 by virtue of its interaction with Raptor through a novel TPTPNPP motif and consequent phosphorylation invitro and in vivo in a Rapamycin-sensitive manner. While we...
Source: Molecular and Cellular Biochemistry - November 28, 2019 Category: Biochemistry Authors: Batool A, Majeed ST, Aashaq S, Majeed R, Bhat NN, Andrabi KI Tags: Mol Cell Biochem Source Type: research

Inhibition of polo-like kinase 1 suppresses microtubule dynamics in MCF-7 cells.
Abstract Polo-like kinase 1 (Plk1) is a mitotic serine/threonine kinase implicated in spindle formation and cytokinesis in mammalian cells. Here, purified Plk1 was found to bind to reconstituted microtubules in vitro. Further, Plk1 was found to co-localize with interphase microtubules in MCF-7 cells and to co-immunoprecipitate with polymerized tubulin. The binding of Plk1 to interphase microtubules appeared to increase with an increase in the level of tubulin acetylation in MCF-7 cells. Interestingly, Plk1 inhibitor III, an inhibitor of Plk1 kinase activity, treatment increased the association of Plk1 with the int...
Source: Molecular and Cellular Biochemistry - November 28, 2019 Category: Biochemistry Authors: Rashid A, Naaz A, Rai A, Chatterji BP, Panda D Tags: Mol Cell Biochem Source Type: research

Identification of the inhibitor of growth protein 4 (ING4) as a potential target in prostate cancer therapy.
This study aimed to assess ING4's role in global transcriptional regulation in PC cells to identify potential cellular processes associated with ING4 loss. RNA-Seq using next-generation sequencing (NGS) was used to identify altered genes in LNCaP PC cells following ING4 depletion. Ingenuity pathways analysis (IPA®) was applied to the data to highlight candidates, ING4-regulated pathways, networks and cellular processes. Selected genes were validated using RT-qPCR. RNA-Seq of LNCaP cells revealed a total of 159 differentially expressed genes (fold change ≥ 1.5 or ≤ - 1.5, FDR&thi...
Source: Molecular and Cellular Biochemistry - November 27, 2019 Category: Biochemistry Authors: Shatnawi A, Malkaram SA, Fandy T, Tsouko E Tags: Mol Cell Biochem Source Type: research

Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells.
In conclusion, the administration of 4000 IU/day of vitD decreased DENV-2 infection. Our findings support a possible role of vitD in improving the innate immune response against DENV. However, further studies are necessary to determine the role of vitD on DENV replication and its innate immune response modulation in MDDCs. PMID: 31758375 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - November 22, 2019 Category: Biochemistry Authors: Martínez-Moreno J, Hernandez JC, Urcuqui-Inchima S Tags: Mol Cell Biochem Source Type: research

Inflammation-induced colon cancer in uPA-deficient mice is associated with a deregulated expression of Notch signaling pathway components.
Abstract Notch is an evolutionarily conserved signaling pathway with an important role in development and cell fate determination. Deregulation of Notch signaling has been associated with several pathological conditions, including cancer. Acting as an oncogene in some types of cancers and as a tumor suppressor in other, Notch effects seem to be highly context-dependent in solid tumors. In the present study, we aimed to investigate gene expression levels of Notch pathway constituents, including ligands, receptors, and target genes, during the early stages of inflammation-associated intestinal carcinogenesis. To ach...
Source: Molecular and Cellular Biochemistry - November 22, 2019 Category: Biochemistry Authors: Afaloniati H, Karagiannis GS, Karavanis E, Psarra TA, Karampatzakis-Kouritas A, Poutahidis T, Angelopoulou K Tags: Mol Cell Biochem Source Type: research

The effect of myeloperoxidase isoforms on biophysical properties of red blood cells.
This study investigated if two MPO isoforms have distinct effects on biophysical properties of RBCs. We have found that hemi-MPO, as well as the dimeric form, bind to the glycophorins A/B and band 3 protein on RBC's plasma membrane, that lead to reduced cell resistance to osmotic and acidic hemolysis, reduction in cell elasticity, significant changes in cell volume, morphology, and the conductance of RBC plasma membrane ion channels. Furthermore, we have shown for the first time that both dimeric and hemi-MPO lead to phosphatidylserine (PS) exposure on the outer leaflet of RBC membrane. However, the effects of hemi-MP...
Source: Molecular and Cellular Biochemistry - November 21, 2019 Category: Biochemistry Authors: Shamova EV, Gorudko IV, Grigorieva DV, Sokolov AV, Kokhan AU, Melnikova GB, Yafremau NA, Gusev SA, Sveshnikova AN, Vasilyev VB, Cherenkevich SN, Panasenko OM Tags: Mol Cell Biochem Source Type: research

RASSF1A-Hippo pathway link in patients with urothelial carcinoma of bladder: plausible therapeutic target.
This study finds a previously unrecognized role of RASSF1A in the regulation of CTGF and CYR61 through mediation of Hippo pathway in UBC and supports the significance of this link as a potential therapeutic target for UBC. PMID: 31754973 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - November 21, 2019 Category: Biochemistry Authors: Khandelwal M, Anand V, Appunni S, Seth A, Singh P, Mathur S, Sharma A Tags: Mol Cell Biochem Source Type: research

Bifidobacterium plays a protective role in TNF- α-induced inflammatory response in Caco-2 cell through NF-κB and p38MAPK pathways.
Bifidobacterium plays a protective role in TNF-α-induced inflammatory response in Caco-2 cell through NF-κB and p38MAPK pathways. Mol Cell Biochem. 2019 Nov 18;: Authors: Nie N, Bai C, Song S, Zhang Y, Wang B, Li Z Abstract Kawasaki disease is an immune-mediated acute, systemic vasculitis and is the leading cause of acquired heart disease in children in the developed world. Bifidobacterium (BIF) is one of the dominant bacteria in the intestines of humans and many mammals and is able to adjust the intestinal flora disorder. The Caco-2 cell monolayers were treated with tumor necrosis factor-...
Source: Molecular and Cellular Biochemistry - November 18, 2019 Category: Biochemistry Authors: Nie N, Bai C, Song S, Zhang Y, Wang B, Li Z Tags: Mol Cell Biochem Source Type: research

Analysis of transporter associated with antigen presentation (TAP) genes polymorphisms with HIV-1 infection.
Abstract Human leukocyte antigen (HLA) class I molecules of the human major histocompatibility complex (MHC) play an important role in modulating immune response. HLA class I molecules present antigenic peptides to CD8+ T cells and thereby play a role in the immune surveillance of cells infected with viruses. TAP1 and TAP2 are MHC-II-encoded genes necessary for the generation of a cellular immune response and polymorphism of these genes can influence the specificity of peptides preferentially presented by the MHC class I molecules and the outcome of the immune response. Several studies implicated genetic variation...
Source: Molecular and Cellular Biochemistry - November 16, 2019 Category: Biochemistry Authors: Abitew AM, Sobti RC, Sharma VL, Wanchu A Tags: Mol Cell Biochem Source Type: research

Ligustrazine suppresses renal NMDAR1 and caspase-3 expressions in a mouse model of sepsis-associated acute kidney injury.
In this study, we investigated the effects of ligustrazine (LGZ) on experimental sepsis-associated AKI in mice. Sepsis-associated AKI was induced in a mice model using cecal ligation and puncture (CLP) method. Mice were administered LGZ (10, 30, and 60 mg/kg) via tail vein injection 0.5 h before CLP surgery. Mice survival was evaluated. Renal water content was detected. Urine samples were collected for ELISA of Kim1. Kidneys were collected for nucleic acid analysis and histological examination. Pathological assessment was used to determine the effect of LGZ on sepsis-associated AKI. Caspase-3 expression in kidney...
Source: Molecular and Cellular Biochemistry - November 16, 2019 Category: Biochemistry Authors: Ying J, Wu J, Zhang Y, Han Y, Qian X, Yang Q, Chen Y, Chen Y, Zhu H Tags: Mol Cell Biochem Source Type: research

Effects of 1,25-Dihydroxy vitamin D3 on TNF- α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Effects of 1,25-Dihydroxy vitamin D3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors. Mol Cell Biochem. 2019 Nov 16;: Authors: Avcioglu G, Özbek Ipteç B, Akcan G, Görgün B, Fidan K, Carhan A, Yilmaz G, Kozaci LD Abstract The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4. The HC and SW1353 cells were trea...
Source: Molecular and Cellular Biochemistry - November 16, 2019 Category: Biochemistry Authors: Avcioglu G, Özbek Ipteç B, Akcan G, Görgün B, Fidan K, Carhan A, Yilmaz G, Kozaci LD Tags: Mol Cell Biochem Source Type: research

Role of inflammation, oxidative stress, and autonomic nervous system activation during the development of right and left cardiac remodeling in experimental pulmonary arterial hypertension.
This study investigated the impact of experimental pulmonary arterial hypertension (PAH) progression by evaluating morphometric and functional parameters, oxidative stress, autonomic nervous system (ANS) activation, and inflammation in the right (RV) and left (LV) ventricles. Male rats were first divided into two groups: monocrotaline (MCT) and control. The MCT group received a single MCT injection (60 mg/kg, intraperitoneal), while control received saline. The MCT and control groups were further divided into four cohorts based on how long they were observed: 1, 2, 3, and 4 weeks. Animals were submitted to echoca...
Source: Molecular and Cellular Biochemistry - November 14, 2019 Category: Biochemistry Authors: Zimmer A, Teixeira RB, Bonetto JHP, Bahr AC, Türck P, de Castro AL, Campos-Carraro C, Visioli F, Fernandes-Piedras TR, Casali KR, Scassola CMC, Baldo G, Araujo AS, Singal P, Belló-Klein A Tags: Mol Cell Biochem Source Type: research

RANKL treatment of vascular endothelial cells leading to paracrine pro-calcific signaling involves ROS production.
Abstract Numerous studies have highlighted the causal link between over-production of reactive oxygen species (ROS) and cardiovascular complications such as vascular calcification (VC). Receptor-activator of nuclear factor-κB ligand (RANKL) has previously been shown to act on endothelial cells, eliciting the production/release of paracrine pro-calcific signals that act, in-turn, upon underlying vascular smooth muscle cells (VSMCs) to induce osteoblastic differentiation and VC. A role for endothelial ROS signaling in this process has not been established however. In the current paper, we investigate the possi...
Source: Molecular and Cellular Biochemistry - November 14, 2019 Category: Biochemistry Authors: Harper E, Rochfort KD, Smith D, Cummins PM Tags: Mol Cell Biochem Source Type: research

Calycosin-7-O- β-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice.
Calycosin-7-O-β-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice. Mol Cell Biochem. 2019 Nov 11;: Authors: Liu Y, Che G, Di Z, Sun W, Tian J, Ren M Abstract Calycosin-7-O-β-D-glucoside (CG) is the component of Astragali Radix, and the aim of the present study is to investigate whether CG protects myocardium from I/R-induced damage by the regulation of IL-10/JAK2/STAT3 signaling pathway. H9C2 cells were subjected to I/R treatment and pretreated with 1 μm CG in vitro. In addition, a mo...
Source: Molecular and Cellular Biochemistry - November 11, 2019 Category: Biochemistry Authors: Liu Y, Che G, Di Z, Sun W, Tian J, Ren M Tags: Mol Cell Biochem Source Type: research

Long non-coding RNA LINC00504 regulates the Warburg effect in ovarian cancer through inhibition of miR-1244.
This study aimed to investigate the function and underlying mechanisms of LINC00504 in OC. The expression levels of LINC00504 in human OC tissues and cell lines were investigated by qRT-PCR analysis. The OC cell proliferation, and apoptosis were evaluated by MTT assay, colony-formation assay, Caspase-3 activity assay, and nucleosome ELISA assay, respectively. The metabolic shift in OC cells was examined by aerobic glycolysis analysis. Dual-luciferase activity reporter assay and mRNA-miRNA pull-down assay were conducted to validate the interaction between LINC00504 and miR-1244. LINC00504 was upregulated in OC cell lines an...
Source: Molecular and Cellular Biochemistry - November 5, 2019 Category: Biochemistry Authors: Liu Y, He X, Chen Y, Cao D Tags: Mol Cell Biochem Source Type: research

CCAAT/enhancer-binding protein beta (C/EBP β) knockdown reduces inflammation, ER stress, and apoptosis, and promotes autophagy in oxLDL-treated RAW264.7 macrophage cells.
CCAAT/enhancer-binding protein beta (C/EBPβ) knockdown reduces inflammation, ER stress, and apoptosis, and promotes autophagy in oxLDL-treated RAW264.7 macrophage cells. Mol Cell Biochem. 2019 Nov 04;: Authors: Zahid MDK, Rogowski M, Ponce C, Choudhury M, Moustaid-Moussa N, Rahman SM Abstract Atherosclerosis is associated with deregulated cholesterol metabolism and formation of macrophage foam cells. CCAAT/enhancer-binding protein beta (C/EBPβ) is a transcription factor, and its inhibition has recently been shown to prevent atherosclerosis development and foam cell formation. However, whethe...
Source: Molecular and Cellular Biochemistry - November 4, 2019 Category: Biochemistry Authors: Zahid MDK, Rogowski M, Ponce C, Choudhury M, Moustaid-Moussa N, Rahman SM Tags: Mol Cell Biochem Source Type: research

In vitro cellular uptake and neuroprotective efficacy of poly-arginine-18 (R18) and poly-ornithine-18 (O18) peptides: critical role of arginine guanidinium head groups for neuroprotection.
This study examined the importance of guanidinium head groups in R18 for peptide cellular uptake, localization, and neuroprotection. This was achieved by using poly-ornithine-18 (O18; 18-mer of ornithine) as a control, which is structurally identical to R18, but possesses amino head groups rather than guanidino head groups. Epifluorescence and confocal fluorescence microscopy was used to examine the cellular uptake and localization of the FITC-conjugated R18 and O18 in primary rat cortical neurons and SH-SY5Y human neuroblastoma cell cultures. An in vitro cortical neuronal glutamic acid excitotoxicity model was used to com...
Source: Molecular and Cellular Biochemistry - November 2, 2019 Category: Biochemistry Authors: MacDougall G, Anderton RS, Ouliel E, Gao J, Redmond SL, Knuckey NW, Meloni BP Tags: Mol Cell Biochem Source Type: research

Heat shock proteins and their expression in primary murine cardiac cell populations during ischemia and reperfusion.
Abstract A tight quality control system over protein folding, turnover and synthesis, involving molecular chaperones and co-chaperones, maintains the balance of cardiac proteins. Various cardiac pathologies, including myocardial infarction, increase stresses and post-translational modifications favoring misfolding due to an overwhelmed quality control system. The toxic nature of accumulated misfolded proteins further worsens the condition. The important molecular chaperones which act as quality control proteins are involved in protecting the heart, these include heat shock protein70 (HSP70) and HSP90. Here, we rev...
Source: Molecular and Cellular Biochemistry - November 1, 2019 Category: Biochemistry Authors: Nair SP, Sharma RK Tags: Mol Cell Biochem Source Type: research

SIRT3 promotes the invasion and metastasis of cervical cancer cells by regulating fatty acid synthase.
Abstract Sirtuin 3 (SIRT3) modulates mitochondria-localized processes and is implicated in the metabolic reprogramming of cancer cells, especially fatty acid (FA) synthesis. However, the relationship between SIRT3 and aberrant lipid synthesis in cervical cancer remains unclear. Here, we investigated the clinical relevance of SIRT3 expression in cervical squamous cell carcinoma (CSCC), cervical intraepithelial neoplasia (CIN), and normal tissues. To analyze the role of SIRT3 in CCSC in vitro, endogenous SIRT3 levels were up- and down-regulated in SiHa and C33a cells, respectively, via lentiviral-based transfection....
Source: Molecular and Cellular Biochemistry - November 1, 2019 Category: Biochemistry Authors: Xu LX, Hao LJ, Ma JQ, Liu JK, Hasim A Tags: Mol Cell Biochem Source Type: research

Pharmacological inhibition of p38 potentiates antimicrobial peptide TP4-induced cell death in glioblastoma cells.
Abstract Glioblastoma is the most common and deadly type of brain cancer. The poor prognosis may be largely attributed to inadequate disease response to current chemotherapeutic agents. Activation of p38 is associated with deleterious outcomes in glioblastoma patients, as its signaling mediates chemoresistance mechanisms. Antimicrobial peptide tilapia piscidin (TP) 4 was identified from Nile tilapia (Oreochromis niloticus) and exhibits strong bactericidal effects on Gram-positive and Gram-negative bacteria. TP4 also has anticancer activity toward human triple-negative breast cancer cells and glioblastoma cell...
Source: Molecular and Cellular Biochemistry - October 31, 2019 Category: Biochemistry Authors: Su BC, Chen JY Tags: Mol Cell Biochem Source Type: research

Overexpression of Shrm4 promotes proliferation and differentiation of neural stem cells through activation of GABA signaling pathway.
Abstract Shrm4 is a protein that is exclusively expressed in polarized tissues. The physiological function of Shrm4 in the brain was required to be elucidated. Thus, we aimed to explore how the Shrm4-mediated gamma-aminobutyric acid (GABA) pathway affected neural stem cells (NSCs). At first, the Nestin expression in cultured NSCs was identified. After determination of the interaction of Shrm4 and GABAB1, a series of in vitro experiment were performed to detect cell proliferation, the ability of cell colony formation, degree that NSCs differentiated into neurons, the apoptosis rate, and cell cycle. The levels of Sh...
Source: Molecular and Cellular Biochemistry - October 25, 2019 Category: Biochemistry Authors: Tian R, Guo K, Wu B, Wang H Tags: Mol Cell Biochem Source Type: research

The m6A methyltransferase METTL3 cooperates with demethylase ALKBH5 to regulate osteogenic differentiation through NF- κB signaling.
The m6A methyltransferase METTL3 cooperates with demethylase ALKBH5 to regulate osteogenic differentiation through NF-κB signaling. Mol Cell Biochem. 2019 Oct 23;: Authors: Yu J, Shen L, Liu Y, Ming H, Zhu X, Chu M, Lin J Abstract As a m6A methylation modifier, METTL3 is functionally involved in various biological processes. Nevertheless, the role of METTL3 in osteogenesis is not determined up to date. In the current study, METTL3 is identified as a crucial regulator in the progression of osteogenic differentiation. Loss of METTL3 significantly augments calcium deposition and enhances alkaline p...
Source: Molecular and Cellular Biochemistry - October 23, 2019 Category: Biochemistry Authors: Yu J, Shen L, Liu Y, Ming H, Zhu X, Chu M, Lin J Tags: Mol Cell Biochem Source Type: research

Beneficial effect of ER stress preconditioning in protection against FFA-induced adipocyte inflammation via XBP1 in 3T3-L1 adipocytes.
Abstract Adipose tissue inflammation is closely associated with the development of obesity and insulin resistance. Free fatty acids (FFAs) are a major inducer of obesity-related insulin resistance. Previously, we reported that endoplasmic reticulum (ER) stress potentially mediated retinal inflammation in diabetic retinopathy. The unfolded protein response (UPR) protects cells against damage induced by oxidative stress. X-box binding protein 1 (XBP1) plays a major role in protecting cells by modulating the UPR. However, the link between ER stress and adipocyte inflammation has been poorly investigated. In the prese...
Source: Molecular and Cellular Biochemistry - October 16, 2019 Category: Biochemistry Authors: Wang M, Chen X, Zheng Z, Yu S, Zhou B, Liu Y, Liu D, Chen Y, Qian X Tags: Mol Cell Biochem Source Type: research

The stimulatory impact of d- δ-Tocotrienol on the differentiation of murine MC3T3-E1 preosteoblasts.
The stimulatory impact of d-δ-Tocotrienol on the differentiation of murine MC3T3-E1 preosteoblasts. Mol Cell Biochem. 2019 Oct 16;: Authors: Shah AK, Yeganehjoo H Abstract Osteoblasts and osteoclasts play essential and opposite roles in maintaining bone homeostasis. Osteoblasts fill cavities excavated by osteoclasts. The mevalonate pathway provides essential prenyl pyrophosphates for the activities of GTPases that promote differentiation of osteoclasts but suppress that of osteoblasts. Preclinical and clinical studies suggest that mevalonate suppressors such as statins increase bone mineral dens...
Source: Molecular and Cellular Biochemistry - October 16, 2019 Category: Biochemistry Authors: Shah AK, Yeganehjoo H Tags: Mol Cell Biochem Source Type: research

Silibinin inhibits migration and invasion of breast cancer MDA-MB-231 cells through induction of mitochondrial fusion.
Abstract Human triple negative breast cancer cells, MDA-MB-231, show typical epithelial to mesenchymal transition associated with cancer progression. Mitochondria play a major role in cancer progression, including metastasis. Changes in mitochondrial architecture affect cellular migration, autophagy and apoptosis. Silibinin is reported to have anti-breast cancer effect. We here report that silibinin at lower concentrations (30-90 μM) inhibits epithelial to mesenchymal transition (EMT) of MDA-MB-231, by increasing the expression of epithelial marker, E-cadherin, and decreasing the expression of mesenchymal ...
Source: Molecular and Cellular Biochemistry - October 14, 2019 Category: Biochemistry Authors: Si L, Fu J, Liu W, Hayashi T, Nie Y, Mizuno K, Hattori S, Fujisaki H, Onodera S, Ikejima T Tags: Mol Cell Biochem Source Type: research

Baicalin augments the differentiation of osteoblasts via enhancement of microRNA-217.
Abstract Baicalin (BAI), a sort of flavonoid monomer, acquires from Scutellaria baicalensis Georgi, which was forcefully reported in diversified ailments due to the pleiotropic properties. But, the functions of BAI in osteoblast differentiation have not been addressed. The intentions of this study are to attest the influences of BAI in the differentiation of osteoblasts. MC3T3-E1 cells or rat primary osteoblasts were exposed to BAI, and then cell viability, ALP activity, mineralization process, and Runx2 and Ocn expression were appraised through implementing CCK-8, p-nitrophenyl phosphate (pNPP), Alizarin red...
Source: Molecular and Cellular Biochemistry - October 12, 2019 Category: Biochemistry Authors: Wang Q, Shi D, Geng Y, Huang Q, Xiang L Tags: Mol Cell Biochem Source Type: research

Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages.
Abstract Adipose-derived stem cells (ADSCs) and their derivatives have aroused intense interest in fields of dermatological and aesthetic medicine. As a major component detected in ADSCs secretome, platelet-derived growth factor AA (PDGF-AA) has been reported mediating extracellular matrix deposition and remodeling, thus might contribute to its anti-aging effect. On the basis of establishing an experimental model that simulate actual skin aging by exposing HDFs to both intrinsic and extrinsic aging factors, we pretreated human dermal fibroblasts (HDFs) with ADSC-conditioned medium (ADSC-CM) before being irradiated...
Source: Molecular and Cellular Biochemistry - October 10, 2019 Category: Biochemistry Authors: Guo S, Wang T, Zhang S, Chen P, Cao Z, Lian W, Guo J, Kang Y Tags: Mol Cell Biochem Source Type: research

Ac154 carried out anti-apoptotic role during AcMNPV infection process in the host insect cells.
In conclusion, our results demonstrated Ac154 could delay apoptosis process in host cells by regulating the transcription of p35 gene and the expression of SfP53 protein, which provided a more favorable environment for progeny virus replication and packaging, thereby promoting the proliferation of progeny virus. So we provided a potentially improved bac-to-bac eukaryotic protein expression system and biopesticide in this work. PMID: 31602540 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 10, 2019 Category: Biochemistry Authors: Bai H, Liang A, Fu Y Tags: Mol Cell Biochem Source Type: research

Long non-coding RNA LINC00243 promotes proliferation and glycolysis in non-small cell lung cancer cells by positively regulating PDK4 through sponging miR-507.
Abstract Non-small cell lung cancer (NSCLC) is the main subtype of lung cancer. The overall survival of NSCLC patients is relatively low even after various treatments. Accumulating evidence demonstrated that long non-coding RNA (LncRNA) plays crucial roles in different biological process. However, the role of a novel LncRNA, LINC00243, in NSCLC remains unclear. We aimed to explore the biological role of LINC00243 in NSCLC. The mRNA and protein levels were determined by real-time PCR and western blot, respectively. Cell viability in vitro was detected by Cell Counting Kit-8 (CCK-8) assay and colony-formation assay....
Source: Molecular and Cellular Biochemistry - October 8, 2019 Category: Biochemistry Authors: Feng X, Yang S Tags: Mol Cell Biochem Source Type: research