Resveratrol increases the activation markers and changes the release of inflammatory cytokines of hepatic stellate cells.
Abstract The phytoalexin Resveratrol (3,5,4'-trihydroxystilbene; RSV) has been related to numerous beneficial effects on health by its cytoprotection and chemoprevention activities. Liver fibrosis is characterized by the extracellular matrix accumulation after hepatic injury and can lead to cirrhosis. Hepatic stellate cells (HSC) play a crucial role during fibrogenesis and liver wound healing by changing their quiescent phenotype to an activated phenotype for protecting healthy areas from damaged areas. Strategies on promoting the activated HSC death, the quiescence return or the cellular activation stimuli decrea...
Source: Molecular and Cellular Biochemistry - October 19, 2020 Category: Biochemistry Authors: de Oliveira CM, Martins LAM, de Sousa AC, Moraes KDS, Costa BP, Vieira MQ, Coelho BP, Borojevic R, de Oliveira JR, Guma FCR Tags: Mol Cell Biochem Source Type: research

Biochemical interaction of salt sensitivity: a key player for the development of essential hypertension.
Abstract Worldwide, more than 1 billion people have elevated blood pressure, with up to 45% of adults affected by the disease. In 2016 the global health study report on patients from 67 countries was released in Lancet, which identified hypertension as the world's leading cause for death and disability-adjusted years since 1990. This paper aims to analyze the pathophysiological connection between hemodynamic inflammatory reactions through sodium balance, salt sensitivity, and potential pathophysiological reactions. Besides, we explore how sodium consumption enhances the expression of transient receptor potent...
Source: Molecular and Cellular Biochemistry - October 18, 2020 Category: Biochemistry Authors: Kazmi I, Al-Maliki WH, Ali H, Al-Abbasi FA Tags: Mol Cell Biochem Source Type: research

Engrafted primary type-2 astrocytes improve the recovery of the nigrostriatal pathway in a rat model of Parkinson's disease.
In this study, we examined the effects of engrafted type-2 astrocytes (T2As) with high expression of FEZ1 on the improvement of the symptoms and functional reconstruction of PD rats. T2As were stereotactically transplanted into the striatum of rats with PD induced by 6-hydroxydopamine (6-OHDA). An examination of apomorphine (APO)-induced rotations was performed to evaluate dopamine neuron damage and motor functions. Remarkably, the grafted cells survived in the lesion environment for six weeks or longer after implantation. In addition, the transplantation of T2As decrease the average velocity and the duration time of the A...
Source: Molecular and Cellular Biochemistry - October 17, 2020 Category: Biochemistry Authors: Sun Y, Lu XJ, Fu X, Zhang Y, Zhan Y, Liu J, Zhao L, Xia CL Tags: Mol Cell Biochem Source Type: research

Statins decrease the expression of c-Myc protein in cancer cell lines.
Abstract Statins are potent inhibitors of the mevalonate/cholesterol biosynthetic pathway and are widely prescribed for the prevention of cardiovascular diseases. Here, we carried out a comprehensive analysis of the effects of three statins, simvastatin, atorvastatin, and lovastatin, on six different cancer cell lines that include a P-glycoprotein-expressing, multidrug resistant variant of an ovarian cancer cell line. Incubation of all cancer cell lines with statins resulted in suppression of cell proliferation without inducing apoptotic cell death. The cell proliferation arrest could be reversed upon transfer of ...
Source: Molecular and Cellular Biochemistry - October 17, 2020 Category: Biochemistry Authors: Rao PS, Rao US Tags: Mol Cell Biochem Source Type: research

SARS-CoV-2 mediated lung inflammatory responses in host: targeting the cytokine storm for therapeutic interventions.
Abstract The recent exposure of novel coronavirus strain, severe acute respiratory syndrome (SARS-CoV-2) has spread to different countries at an alarming rate. Faster transmission rate and genetic modifications have provoked scientists to search for an immediate solution. With an increasing death rate, it becomes important to throw some light on the life cycle of the virus and its associated pathogenesis in the form of lung inflammation through cytokine storm (CS) production. This paper highlights the different stages of viral-mediated inflammatory responses in the host respiratory system. Previously, known anti-i...
Source: Molecular and Cellular Biochemistry - October 16, 2020 Category: Biochemistry Authors: Asrani P, Hassan MI Tags: Mol Cell Biochem Source Type: research

R-sulforaphane modulates the expression profile of AhR, ER α, Nrf2, NQO1, and GSTP in human breast cell lines.
This study aimed to evaluate the effect of R-SFN on phase II enzymes induction and expression of AhR, Nrf2, and ERα in the same breast cell lines. The results showed increased expression of GSTP as a result of treatment with R-SFN in breast cancer cells. An increased NQO1 transcript and protein levels were found in all breast cells, with the most significant increase in MCF7 cells. Similarly, the enhancement of Nrf2 expression was noticed in all tested cells. AhR gene transcript and protein were decreased in MCF7 cells. In MDA-MB-231, increased AhR mRNA was not confirmed at the protein level. No differences were foun...
Source: Molecular and Cellular Biochemistry - October 16, 2020 Category: Biochemistry Authors: Licznerska B, Szaefer H, Krajka-Kuźniak V Tags: Mol Cell Biochem Source Type: research

Epigenetic memory: gene writer, eraser and homocysteine.
Abstract Naturally chromatin remodeling is highly organized, consisting of histone acetylation (opening/relaxation of the compact chromatin structure), DNA methylation (inhibition of the gene expression activity) and sequence rearrangement by shifting. All this is essentially required for proper "in-printing and off-printing" of genes thus ensuring the epigenetic memory process. Any imbalance in ratios of DNA methyltransferase (DNMT, gene writer), fat-mass obesity-associated protein (FTO, gene eraser) and product (function) homocysteine (Hcy) could lead to numerous diseases. Interestingly, a similar proc...
Source: Molecular and Cellular Biochemistry - October 8, 2020 Category: Biochemistry Authors: Tyagi SC, Stanisic D, Singh M Tags: Mol Cell Biochem Source Type: research

Role of ACE2 receptor and the landscape of treatment options from convalescent plasma therapy to the drug repurposing in COVID-19.
Abstract Since the first case reports in Wuhan, China, the SARS-CoV-2 has caused a pandemic and took lives of > 8,35,000 people globally. This single-stranded RNA virus uses Angiotensin-converting enzyme 2 (ACE2) as a receptor for entry into the host cell. Overexpression of ACE2 is mainly observed in hypertensive, diabetic and heart patients that make them prone to SARS-CoV-2 infection. Mitigations strategies were opted globally by the governments to minimize transmission of SARS-CoV-2 via the implementation of social distancing norms, wearing the facemasks, and spreading awareness using digital p...
Source: Molecular and Cellular Biochemistry - October 7, 2020 Category: Biochemistry Authors: Kumar P, Sah AK, Tripathi G, Kashyap A, Tripathi A, Rao R, Mishra PC, Mallick K, Husain A, Kashyap MK Tags: Mol Cell Biochem Source Type: research

AGE-RAGE  synergy influences programmed cell death signaling to promote cancer.
AGE-RAGE synergy influences programmed cell death signaling to promote cancer. Mol Cell Biochem. 2020 Oct 06;: Authors: Waghela BN, Vaidya FU, Ranjan K, Chhipa AS, Tiwari BS, Pathak C Abstract Advanced glycation end products (AGEs) are formed as a result of non-enzymatic reaction between the free reducing sugars and proteins, lipids, or nucleic acids. AGEs are predominantly synthesized during chronic hyperglycemic conditions or aging. AGEs interact with their receptor RAGE and activate various sets of genes and proteins of the signal transduction pathway. Accumulation of AGEs and upregulated expr...
Source: Molecular and Cellular Biochemistry - October 6, 2020 Category: Biochemistry Authors: Waghela BN, Vaidya FU, Ranjan K, Chhipa AS, Tiwari BS, Pathak C Tags: Mol Cell Biochem Source Type: research

The functional interplay of transcription factors and cell adhesion molecules in experimental myelodysplasia including hematopoietic stem progenitor compartment.
Abstract Myelodysplastic syndrome is a heterogenous group of disorder with clonal dysregulated hematopoiesis characterized by bone marrow failure, cytogenetic and molecular abnormalities and variable risk of progression to acute myeloid leukemia (AML). The bone marrow niche plays a major role in maintaining the homeostasis and is often injured by the chemotherapeutic drugs leading to catastrophic consequences like myelodysplastic syndrome. In the present study, we made an attempt to find out the osteoblastic niche related alterations in the myelodysplastic bone marrow through mainly flowcytometric and fluorescent ...
Source: Molecular and Cellular Biochemistry - October 3, 2020 Category: Biochemistry Authors: Daw S, Law S Tags: Mol Cell Biochem Source Type: research

Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer  HCT-8/Fu cells.
Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. Mol Cell Biochem. 2020 Oct 03;: Authors: Shan S, Lu Y, Zhang X, Shi J, Li H, Li Z Abstract Nature polyphenols widely present in plants and foods are promising candidates in cancer chemotherapy. Emerging evidence has shown that plant polyphenols regulate the expression of miRNAs to exert the anti-Multidrug resistance (MDR) activity, which partly attributes to their regulation on miRNAs methylation. Our previous study found that bound polyph...
Source: Molecular and Cellular Biochemistry - October 2, 2020 Category: Biochemistry Authors: Shan S, Lu Y, Zhang X, Shi J, Li H, Li Z Tags: Mol Cell Biochem Source Type: research

Telomerase activation in the treatment of aging or degenerative diseases: a systematic review.
Abstract Telomeres are protective structures that are shortened during the lifetime, resulting in aging and degenerative diseases. Subjects experiencing aging and degenerative disorders present smaller telomeres than young and healthy ones. The size of these structures can be stabilized by telomerase, an enzyme which is inactive in adult tissues but functional in fetal and newborn tissues and adult testes and ovaries. The aim of this study was to perform a systematic review to evaluate the effect of telomerase activation in the treatment of degenerative and aging disorders. We accomplished the search using the Pub...
Source: Molecular and Cellular Biochemistry - September 30, 2020 Category: Biochemistry Authors: Prieto-Oliveira P Tags: Mol Cell Biochem Source Type: research

Recent progress in the use of mitochondrial membrane permeability transition pore in mitochondrial dysfunction-related disease therapies.
Abstract Mitochondria have various cellular functions, including ATP synthesis, calcium homeostasis, cell senescence, and death. Mitochondrial dysfunction has been identified in a variety of disorders correlated with human health. Among the many underlying mechanisms of mitochondrial dysfunction, the opening up of the mitochondrial permeability transition pore (mPTP) is one that has drawn increasing interest in recent years. It plays an important role in apoptosis and necrosis; however, the molecular structure and function of the mPTP have still not been fully elucidated. In recent years, the abnormal opening up o...
Source: Molecular and Cellular Biochemistry - September 29, 2020 Category: Biochemistry Authors: Cui Y, Pan M, Ma J, Song X, Cao W, Zhang P Tags: Mol Cell Biochem Source Type: research

Reactivation of fatty acid oxidation by medium chain fatty acid prevents myocyte hypertrophy in H9c2 cell line.
In conclusion, the beneficial effect of MCFA is possibly mediated by stimulation of fatty acid metabolism and modulation of MAPK and Calcineurin A. PMID: 33000353 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - September 29, 2020 Category: Biochemistry Authors: Ismael S, Nair RR Tags: Mol Cell Biochem Source Type: research

miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells.
In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro...
Source: Molecular and Cellular Biochemistry - September 29, 2020 Category: Biochemistry Authors: Shi Y, Mao X, Cai M, Hu S, Lai X, Chen S, Jia X, Wang J, Lai S Tags: Mol Cell Biochem Source Type: research

A comparative study on the cellular stressors in mesenchymal stem cells (MSCs) and pancreatic β-cells under hyperglycemic milieu.
A comparative study on the cellular stressors in mesenchymal stem cells (MSCs) and pancreatic β-cells under hyperglycemic milieu. Mol Cell Biochem. 2020 Sep 30;: Authors: Raghavan S, Malayaperumal S, Mohan V, Balasubramanyam M Abstract β-cell dysfunction is a critical determinant for both type 1 diabetes and type 2 diabetes and β-cells are shown to be highly susceptible to cellular stressors. Mesenchymal stem cells (MSCs) on the other hand are known to have immunomodulatory potential and preferred in clinical applications. However, there is paucity of a comparative study on these cells ...
Source: Molecular and Cellular Biochemistry - September 29, 2020 Category: Biochemistry Authors: Raghavan S, Malayaperumal S, Mohan V, Balasubramanyam M Tags: Mol Cell Biochem Source Type: research

Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies.
Abstract Ataxia telangiectasia mutated (ATM), a critical DNA damage sensor, also possesses non-nuclear functions owing to its presence in extra-nuclear compartments, including peroxisomes, lysosomes, and mitochondria. ATM is frequently altered in several human cancers. Recently, we and others have shown that loss of ATM is associated with defective mitochondrial autophagy (mitophagy) in ataxia-telangiectasia (A-T) fibroblasts and B-cell lymphomas. Further, we reported that ATM protein but not ATM kinase activity is required for mitophagy. However, the mechanism of ATM kinase activation during ionophore-induced mit...
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Sarkar A, Gandhi V Tags: Mol Cell Biochem Source Type: research

Long non-coding RNA HCG18 promotes M1 macrophage polarization through regulating the miR-146a/TRAF6 axis, facilitating the progression of diabetic peripheral neuropathy.
This study provides a possible therapeutic strategy for DPN. PMID: 32996080 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Ren W, Xi G, Li X, Zhao L, Yang K, Fan X, Gao L, Xu H, Guo J Tags: Mol Cell Biochem Source Type: research

Memory impairment of chewing-side preference mice is associated with 5-HT-BDNF signal pathway.
This study evaluates the behavior changes in mice after the loss of teeth on one side and explores the role of serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) signal pathway within these changes. To this end, CSP mouse models with either the removal of left unilateral molars (CSP-L) or right unilateral molars (CSP-R) were established. Morris water maze test and passive avoidance test were performed to evaluate the mice's learning and memory capacity in the 4th and 8th weeks. The correlation between CSP and brain function changes was validated with changes in 5-HT and BDNF levels. CSP mice's cognitive function...
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Jiang H, Yin H, Wang L, Feng C, Bai Y, Huang D, Zhang Q, Liu H, Hu Y Tags: Mol Cell Biochem Source Type: research

KCNQ1OT1/miR-18b/HMGA2 axis regulates high glucose-induced proliferation, oxidative stress, and extracellular matrix accumulation in mesangial cells.
Abstract The dysregulated long noncoding RNAs (lncRNAs) are associated with the pathogenesis of diabetic nephropathy (DN). LncRNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) plays an important role in diabetes, but the role and mechanism of KCNQ1OT1 in DN are largely unknown. Serum samples were collected from 30 DN patients and normal volunteers. High glucose (HG)-challenged human mesangial cells (HMCs) were used as a cell model of DN. KCNQ1OT1, microRNA-18b (miR-18b), and high mobility group protein A2 (HMGA2) abundances were examined via quantitative reverse transcrip...
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Li J, Li M, Bai L Tags: Mol Cell Biochem Source Type: research

Utilization of serum procalcitonin as a biomarker in the diagnosis and treatment of children with bacterial hospital-acquired pneumonia.
Abstract Hospital-acquired pneumonia (HAP) is one of the common infections in hospitalized patients. Early and prompt diagnosis of HAP is important because it aids in the appropriate selection of antibiotics and decreases the mortality and morbidity of patients. The investigation on serum procalcitonin (PCT) levels in pediatric patients is limited. Herein we aimed to evaluate the role of PCT in the early diagnosis of children with bacterial HAP. The study enrolled 264 children (
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Wang W, Zhu Y, Yin L, Deng Y, Chu G, Liu S Tags: Mol Cell Biochem Source Type: research

LncRNA OIP5-AS1 facilitates ox-LDL-induced endothelial cell injury through the miR-98-5p/HMGB1 axis.
Abstract Cerebrovascular diseases have a high mortality and disability rate in developed countries. Endothelial cell injury is the main cause of atherosclerosis and cerebrovascular disease. Long non-coding RNA (lncRNA) has been proved to participate in the progression of endothelial cell. Our study aimed to develop the function of lncRNA opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) in oxidative low-density lipoprotein (ox-LDL)-induced endothelial cell injury. The expression of OIP5-AS1, miR-98-5p and High-mobility group protein box-1 (HMGB1) was detected by quantitative real-time polymerase chain reaction ...
Source: Molecular and Cellular Biochemistry - September 28, 2020 Category: Biochemistry Authors: Zheng Z, Zhang G, Liang X, Li T Tags: Mol Cell Biochem Source Type: research

The development and controversy of competitive endogenous RNA hypothesis in non-coding genes.
Abstract As a momentous post-transcriptional regulator, microRNAs (miRNAs) are attracting more and more attention. The classical miRNAs regulated mechanism shows it binds to the targets' 3'UTR thus play the role in post-transcription. Meanwhile, single miRNA can target multiple genes, so those should compete to bind that miRNA. Vice versa, single gene can sponge mass of miRNAs as well. Thus the competitive endogenous RNAs (ceRNAs) hypothesis was put forward in 2011. The ceRNA hypothesis has made huge achievements, in particular in non-coding genes, which including long non-coding RNAs (lncRNAs), circle RNAs (circR...
Source: Molecular and Cellular Biochemistry - September 24, 2020 Category: Biochemistry Authors: Lin W, Liu H, Tang Y, Wei Y, Wei W, Zhang L, Chen J Tags: Mol Cell Biochem Source Type: research

Allicin can suppress the activity of vascular endothelial cells probably by regulating JAK2/STAT3 pathway.
Abstract Whether allicin can suppress the angiogenesis via inhibiting the activity of vascular endothelial cells (VECs) in preventing epidural hypertrophic scars remains unknown. VECs were treated by allicin at a gradient of concentrations. Cell activity was measured by CCK-8 assay, scratch assay and flow cytometry. Reverse-transcription PCR and Western Blot were used to measure the expression levels of relevant genes and proteins. After treated with allicin at concentrations of 0, 25, 50 and 100 mg/L, the viability of VECs significantly decreased at 24 h (p 
Source: Molecular and Cellular Biochemistry - September 24, 2020 Category: Biochemistry Authors: Sun HH, Feng XM, Wang JC, Cai J Tags: Mol Cell Biochem Source Type: research

Tumor suppressor genes are differentially regulated with dietary folate modulations in a rat model of hepatocellular carcinoma.
Abstract The current study evaluated the outcome of dietary folate modulations on the expression of tumor suppressor genes (TSGs) during developmental stages of hepatocellular carcinoma (HCC) in a Wistar rat model. In addition to dietary folate modulations, male rats were administered diethylnitrosamine (DEN) intraperitoneally once a week upto 18 weeks to induce HCC. Serum folate levels were found to be decreased and increased in folate deficiency (FD) and folate-oversupplemented (FO) groups respectively when compared to folate normal (FN) rats. Apoptosis was observed in FD in fibrosis and HCC stages. mRNA ex...
Source: Molecular and Cellular Biochemistry - September 23, 2020 Category: Biochemistry Authors: Sharma R, Ali T, Kaur J Tags: Mol Cell Biochem Source Type: research

MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice.
Abstract Matrix Metalloproteinases (MMPs)-induced altered proteolysis of extracellular matrix proteins and basement membrane holds the key for tumor progression and metastasis. Matrix metalloproteinases-7 (Matrilysin), the smallest member of the MMP family also performs quite alike; thus serves as a potential candidate for anti-tumor immunotherapy. Conversely, being an endogenous tumor-associated antigen (TAA), targeting MMP-7 for immunization is challenging. But MMP-7-based xenovaccine can surmount the obstacle of poor immunogenicity and immunological tolerance, often encountered in TAA-based conventional vaccine...
Source: Molecular and Cellular Biochemistry - September 23, 2020 Category: Biochemistry Authors: Yadav PK, Gupta SK, Kumar S, Ghosh M, Yadav BS, Kumar D, Kumar A, Saini M, Kataria M Tags: Mol Cell Biochem Source Type: research

CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p.
Abstract Circular RNA zinc finger protein 609 (circZNF609) has been reported to involve in nasopharyngeal carcinoma (NPC) tumorigenesis regulation. However, the role and the molecular mechanism of circZNF609 in NPC remain unclear. Levels of circZNF609, microRNA (miR)-338-3p, and GTPase HRas (HRAS) were detected by quantitative real-time polymerase chain reaction or Western blot. Cell proliferation, migration, and invasion were analyzed using cell counting kit-8 assay, colony formation assay, and transwell assay, respectively. Glucose metabolism was calculated by measuring glucose consumption, lactate production, a...
Source: Molecular and Cellular Biochemistry - September 23, 2020 Category: Biochemistry Authors: Liu Z, Liu F, Wang F, Yang X, Guo W Tags: Mol Cell Biochem Source Type: research

Effects of pulsed electrical stimulation on growth factor gene expression and proliferation in human dermal fibroblasts.
In conclusion, PES may activate the cell growth phase of wound healing. PMID: 32968926 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - September 22, 2020 Category: Biochemistry Authors: Urabe H, Akimoto R, Kamiya S, Hosoki K, Ichikawa H, Nishiyama T Tags: Mol Cell Biochem Source Type: research

Gallic acid ameliorates COPD-associated exacerbation in mice.
Abstract COPD is an inflammatory lung disease, which is often exacerbated with microbial infections resulting in worsening of respiratory symptoms. Gallic acid (GA), a naturally occurring phenolic compound is known to possess anti-oxidant/anti-inflammatory activity. We have recently reported that GA protects against the elastase (ET) induced lung inflammation and emphysema and the present work was designed to investigate the beneficial effects of Gallic acid against ET + Lipopolysachharide (LPS) induced COPD exacerbation like condition in mice model. Our data showed that i.t. administration of LPS at...
Source: Molecular and Cellular Biochemistry - September 22, 2020 Category: Biochemistry Authors: Singla E, Puri G, Dharwal V, Naura AS Tags: Mol Cell Biochem Source Type: research

Calcitriol attenuates TLR2/IL-33 signaling pathway to repress Th9 cell differentiation and potentially limits the pathophysiology of rheumatoid arthritis.
Abstract There is limited information regarding the TLR2 signaling pathway involved in Th9 cell differentiation. The role of calcitriol in regulating TLR2-mediated Th9 cell development is unknown. Thus, we aimed to unravel the TLR2 signaling pathway in Th9 cells and its regulation by calcitriol. We have used n = 5-6 animals for each murine experiment. Human studies involved five healthy volunteers. Moreover, ten healthy individuals and ten RA patients were included in the study. Murine and human Th9 cells were treated with Calcitriol (100 nM) and Pam3CSK4 (2 µg/mL). The number of IL-9...
Source: Molecular and Cellular Biochemistry - September 22, 2020 Category: Biochemistry Authors: Vyas SP, Srivastava RN, Goswami R Tags: Mol Cell Biochem Source Type: research

LncRNA MALAT1 mediates doxorubicin resistance of hepatocellular carcinoma by regulating miR-3129-5p/Nova1 axis.
This study would be expected to enrich the understanding of doxorubicin resistance of HCC and provide new ideas for HCC treatment strategies. PMID: 32965597 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - September 22, 2020 Category: Biochemistry Authors: Cao Y, Zhang F, Wang H, Bi C, Cui J, Liu F, Pan H Tags: Mol Cell Biochem Source Type: research

Maternal cholesterol levels during gestation: boon or bane for the offspring?
Abstract An increase in cholesterol levels is perceived during pregnancy and is considered as a normal adaptive response to the development of the fetus. In some pregnancies, excessive increase in total cholesterol with high levels of Low-Density Lipoprotein leads to maladaptation by the fetus to cholesterol demands, resulting in a pathological condition termed as maternal hypercholesterolemia (MH). MH is considered clinically irrelevant and therefore cholesterol levels are not routinely checked during pregnancy, as a consequence of which there is scarce information on its global prevalence in pregnant women. Stud...
Source: Molecular and Cellular Biochemistry - September 21, 2020 Category: Biochemistry Authors: Jayalekshmi VS, Ramachandran S Tags: Mol Cell Biochem Source Type: research

The anticarcinogen activity of β-arbutin on MCF-7 cells: Stimulation of apoptosis through estrogen receptor-α signal pathway, inflammation and genotoxicity.
The anticarcinogen activity of β-arbutin on MCF-7 cells: Stimulation of apoptosis through estrogen receptor-α signal pathway, inflammation and genotoxicity. Mol Cell Biochem. 2020 Sep 22;: Authors: Hazman Ö, Sarıova A, Bozkurt MF, Ciğerci İH Abstract Arbutin is one of the active ingredients employed in cosmetics as a skin whitening agent. In the present study, the possible effects of arbutin on breast cancer were determined with human breast adenocarcinoma (MCF-7) cells. α and β-arbutin cytotoxicity levels in MCF-7 cells were determined with the MTT method. At low (1-10&n...
Source: Molecular and Cellular Biochemistry - September 21, 2020 Category: Biochemistry Authors: Hazman Ö, Sarıova A, Bozkurt MF, Ciğerci İH Tags: Mol Cell Biochem Source Type: research

Circular RNA circ_HN1 facilitates gastric cancer progression through modulation of the miR-302b-3p/ROCK2 axis.
In conclusion, circ_HN1 exerted an oncogenic role in GC through upregulating ROCK2 via sponging miR-302b-3p, offering evidence that circ_HN1 is a potential target for GC therapy. PMID: 32949310 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - September 18, 2020 Category: Biochemistry Authors: Wang D, Jiang X, Liu Y, Cao G, Zhang X, Kuang Y Tags: Mol Cell Biochem Source Type: research

Chemical components of ginseng, their biotransformation products and their potential as treatment of hypertension.
Abstract Ginseng is an ancient perennial herb belonging to the family Araliaceae and genus Panax which has been used for medical therapeutics for thousands of years, particularly in China and other Asian cultures although increasing interest in ginseng has recently emerged in western societies. Ginseng is a complex substance containing dozens of bioactive and potentially effective therapeutic compounds. Among the most studied are the ginsenosides, which are triterpene saponins possessing a wide array of potential therapeutic effects for many conditions. The quantity and type of ginsenoside vary greatly depending o...
Source: Molecular and Cellular Biochemistry - September 16, 2020 Category: Biochemistry Authors: Karmazyn M, Gan XT Tags: Mol Cell Biochem Source Type: research

Heat shock protein B8 promotes proliferation and migration in lung adenocarcinoma A549 cells by maintaining mitochondrial function.
Abstract Heat shock protein B8 (HSPB8) impacts on tumor proliferation and migration of malignancy. However, the role of HSPB8 in lung adenocarcinoma (LUAC) remains unclear. The aim of this study, therefore, was to clarify whether HSPB8 could bring benefits to proliferation and migration of LUAC and its underlying mechanisms. The expression of HSPB8 was first evaluated by immunohistochemistry in 35 LUAC samples. Then, A549 lung adenocarcinoma cells were transfected with pcDNA-HSPB8 or si-HSPB8 to induce HSPB8 overexpression and silence. Cellular activity was evaluated with a Cell Counting Kit-8 (CCK-8) assay. Cell ...
Source: Molecular and Cellular Biochemistry - September 13, 2020 Category: Biochemistry Authors: Yu LL, Wang Y, Xiao ZK, Chen SS Tags: Mol Cell Biochem Source Type: research

The effect of aging on VEGF/VEGFR2 signal pathway genes expression in rat liver sinusoidal endothelial cell.
Abstract Liver sinusoidal endothelial cells (LSECs) play a key role in the initiation and neoangiogenesis of liver regeneration. We presume that the abnormity of the VEGF/VEGFR2 and its pathway gene Id1, Wnt2 and HGF expression in aged LSECs may be an important mechanism to affect liver regeneration of the elderly. LSECs from two different groups (adult and old) were isolated in a rodent model, and observed by SEM and TEM. The adult and old rats were underwent 70% partial hepatectomy. The proliferation of hepatocytes and LSECs were analyzed by Immunofluorescence staining. The expression of VEGF/VEGFR2 and its path...
Source: Molecular and Cellular Biochemistry - September 11, 2020 Category: Biochemistry Authors: Wang WL, Zheng XL, Li QS, Liu WY, Hu LS, Sha HC, Guo K, Lv Y, Wang B Tags: Mol Cell Biochem Source Type: research

Calorie restriction delays cardiac senescence and improves cardiac function in obese diabetic rats.
Abstract The aims of this study were to investigate the impact of caloric restriction (CR) on cardiac senescence in an animal model of diabetes and examine the signal transduction mechanisms for changes in cell survival as well as cardiac function. Male 8-week-old Otsuka Long-Evans Tokushima fatty (OLETF) diabetic rats were divided into 2 groups: a group fed ad libitum (AL), and a group fed with CR (30% energy reduction). Long-Evans Tokushima Otsuka (LETO) non-diabetic rats were used as controls. LETO rats were divided into 3 groups: a high fat diet (HFD) group with a 22% increase in caloric intake, a CR group, an...
Source: Molecular and Cellular Biochemistry - September 11, 2020 Category: Biochemistry Authors: Makino N, Maeda T Tags: Mol Cell Biochem Source Type: research

Role of Nrf2 in cell senescence regulation.
Abstract Nuclear factor-E2-related factor 2 (Nrf2) is a key transcription factor known to be involved in maintaining cell redox balance and signal transduction and plays central role in reducing intracellular oxidative stress damage, delaying cell senescence and preventing age-related diseases. However, it has been shown that the level of Nrf2 decreases with age and that the silencing of the Nrf2 gene is associated with the induction of premature senescence. Therefore, a plethora of researchers have focused on elucidating the regulatory mechanism of Nrf2 in the prevention of cell senescence. This complex regulator...
Source: Molecular and Cellular Biochemistry - September 10, 2020 Category: Biochemistry Authors: Yuan H, Xu Y, Luo Y, Wang NX, Xiao JH Tags: Mol Cell Biochem Source Type: research

The emerging roles of circular RNAs in regulating the fate of stem cells.
Abstract Circular RNAs(circRNAs) are a large family of RNAs shaping covalently closed ring-like molecules and have become a hotspot with thousands of newly published studies. Stem cells are undifferentiated cells and have great potential in medical treatment due to their self-renewal ability and differentiation capacity. Abundant researches have unveiled that circRNAs have unique expression profile during the differentiation of stem cells and could serve as promising biomarkers of these cells. There are key circRNAs relevant to the differentiation, proliferation, and apoptosis of stem cells with certain mechanisms...
Source: Molecular and Cellular Biochemistry - September 10, 2020 Category: Biochemistry Authors: Zhuang Z, Jia L, Li W, Zheng Y Tags: Mol Cell Biochem Source Type: research

Identification of the soluble EphA7-interacting protein Nicalin as a regulator of EphA7 expression.
In this study, we identified Nicalin, a Nicastrin-like protein, as a novel sEphA7-interacting protein using immunoprecipitation (IP)/mass spectrometry (MS). In HEK293 cells, Nicalin interacted with sEphA7 and they predominantly co-localized in the endoplasmic reticulum (ER). Interestingly, Nicalin diminished the protein level of sEphA7 in the membranous fraction but increased that in the insoluble cytoplasmic fraction with a reduced molecular weight, suggesting that Nicalin restricts the entry of sEphA7 into the ER for further modification. sEphA7 probably acted as a chaperone and enhanced the membrane level of EphA7-FL an...
Source: Molecular and Cellular Biochemistry - September 9, 2020 Category: Biochemistry Authors: Wang X, Wang Z Tags: Mol Cell Biochem Source Type: research

LncRNA TUG1 reduces inflammation and enhances insulin sensitivity in white adipose tissue by regulating miR-204/SIRT1 axis in obesity mice.
This study aimed to explore the mechanism of long non-coding RNA TUG/miR-204/SIRT1 axis, which was involved in the pathogenesis of obesity. Obesity mouse model was induced by high-fat diet and treated with taurine upregulated gene1 (TUG1) virus via tail intravenous injection. Then, body weight, serum glucose, insulin tolerance, testicular fat weight were detected, as well as the expression of TUG1, microRNA-204 (miR-204), sirtuin1 (SIRT1), and inflammation and fatty accumulation associated proteins and cytokines. Regulatory relationship between TUG1/SIRT1 and miR-204 was confirmed by dual-luciferase reporter activity assay...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Zhang Y, Gu M, Ma Y, Peng Y Tags: Mol Cell Biochem Source Type: research

Chromosomal terminal methylation status is associated with gut microbiotic alterations.
Abstract We explored the association of fecal bacterial species and somatic telomere changes in patients with chronic disease. The results showed that the length of the combined range of telomere and the methylated subtelomere was correlated with the increase of bacteria species and the numerical superiority of certain strains in feces, the increase of streptococci in men and women, and the increase of E. coli specifically in women. These results suggest that the aging status reflected by telomere length and/or demethylation of neighboring regions correlate with intestinal conditions which influences the proportio...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Maeda T, Horiuchi T, Makino N Tags: Mol Cell Biochem Source Type: research

New perspectives in triple-negative breast cancer therapy based on treatments with TGF β1 siRNA and doxorubicin.
In this study, a combined doxorubicin (DOX) and small interfering RNA (siRNA) therapy is proposed as therapeutic strategy for targeting TGFβ1 gene. Hs578T cell line is used as in vitro model for TNBC, wherein TGFβ1siRNA therapy is employed to enhance therapeutic effects. Cell proliferation rate is measured using an MTT test, and morphological alterations are assed using microscopically approached, while gene expression is determined by qRT-PCR analysis. The combined treatment of TGFβ1siRNA and DOX reduced levels of cell proliferation and mitochondrial activity and promoted the alteration of cell morphology (...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Ciocan-Cȃrtiţă CA, Jurj A, Raduly L, Cojocneanu R, Moldovan A, Pileczki V, Pop LA, Budişan L, Braicu C, Korban SS, Berindan-Neagoe I Tags: Mol Cell Biochem Source Type: research

LncRNA TTTY15 knockdown alleviates H2O2-stimulated myocardial cell injury by regulating the miR-98-5p/CRP pathway.
Abstract Acute myocardial infarction (AMI) can lead to myocardial injury, and long non-coding RNA (lncRNA) has been found to play an important regulatory role in the process of myocardial injury. However, the role and potential mechanisms of lncRNA testis-specific transcript Y-linked 15 (TTTY15) in AMI-induced myocardial injury has not been fully elucidated. Hydrogen peroxide (H2O2)-induced AMI cell model was built and AMI mice model were constructed. Relative expression levels of TTTY15, miR-98-5p and C-reactive protein (CRP) were determined by quantitative real-time PCR (qRT-PCR). Cell counting kit 8 (CCK8) assa...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Ma R, Gao L, Liu Y, Du P, Chen X, Li G Tags: Mol Cell Biochem Source Type: research

Ovariectomized rodents as a menopausal metabolic syndrome model. A minireview.
Abstract Bilateral ovariectomy is the best characterized and the most reported animal model of human menopause. Ovariectomized rodents develop insulin resistance (IR) and visceral obesity, the main risk factors in the pathophysiology of metabolic syndrome (MS). These alterations are a consequence of hypoestrogenic status, which produces an augment of visceral fat, high testosterone levels (hyperandrogenism), as well as inflammation, oxidative stress, and metabolic complications, such as dyslipidemia, hepatic steatosis, and endothelial dysfunction, among others. Clinical trials have reported that menopause per se i...
Source: Molecular and Cellular Biochemistry - August 26, 2020 Category: Biochemistry Authors: Medina-Contreras J, Villalobos-Molina R, Zarain-Herzberg A, Balderas-Villalobos J Tags: Mol Cell Biochem Source Type: research

Recent updates on novel therapeutic targets of cardiovascular diseases.
Abstract In recent times cardiovascular diseases (CVDs) are the leading cause of mortality universally, caused more or less 17.7 million casualties with 45% of all illnesses (except communicable ones) in 2015 as per World Health Organization (WHO). According to American National Center for Health Statistics, cardiac disorders are costliest. Moreover, health care expenditures related to cardiac disorders are anticipated to exceed than diabetes and Alzheimer's. Straining of reactive oxygen species with diminished neutralization & inflammation critically adds to atherosclerosis and also proceed to other cardiovas...
Source: Molecular and Cellular Biochemistry - August 25, 2020 Category: Biochemistry Authors: Afzal M Tags: Mol Cell Biochem Source Type: research

Studies on the effects of bone marrow stem cells on mitochondrial function and the alleviation of ARDS.
Abstract Mesenchymal stem cells (MSCs) can alleviate acute respiratory distress syndrome (ARDS), but the mechanisms involved are unclear, especially about their specific effects on cellular mitochondrial respiratory function. Thirty mice were allocated into the Control, LPS, and LPS + Bone marrow mesenchymal stem cell (BMSC) group (n = 10/group). Mouse alveolar epithelial cells (MLE-12) and macrophage cells (RAW264.7) were divided into the same groups. Pathological variation, inflammation-related factors, reactive oxygen species (ROS), ATP levels, and oxygen consumption rate (OCR) were ...
Source: Molecular and Cellular Biochemistry - August 25, 2020 Category: Biochemistry Authors: Zhang K, Gao Y, Deng Y, Zhou X, Zhu C, He Z, Lv D Tags: Mol Cell Biochem Source Type: research

Myricetin: versatile plant based flavonoid for cancer treatment by inducing cell cycle arrest and ROS-reliant mitochondria-facilitated apoptosis in A549 lung cancer cells and in silico prediction.
Abstract Myricetin is categorized under the secondary metabolite flavonoid which includes a diverse range of consumable plant parts, and it has a potential against several classes of cancer including cancers and tumors. In the present study, the anticancer potential of the unique flavonoid-myricetin in A549 lung cancer cells was evaluated. Among different doses of myricetin, 73 μg/ml was more effective to prevent the cancer cell growth. It also promoted sub-G1 phase aggregation of cells and a equivalent decrease in the fraction of cells entering the S and subsequent phase which indicates apoptotic cell dea...
Source: Molecular and Cellular Biochemistry - August 25, 2020 Category: Biochemistry Authors: Rajendran P, Maheshwari U, Muthukrishnan A, Muthuswamy R, Anand K, Ravindran B, Dhanaraj P, Balamuralikrishnan B, Chang SW, Chung WJ Tags: Mol Cell Biochem Source Type: research

Hemodynamics mediated epigenetic regulators in the pathogenesis of vascular diseases.
Abstract Endothelium of blood vessels is continuously exposed to various hemodynamic forces. Flow-mediated epigenetic plasticity regulates vascular endothelial function. Recent studies have highlighted the significant role of mechanosensing-related epigenetics in localized endothelial dysfunction and the regional susceptibility for lesions in vascular diseases. In this article, we review the epigenetic mechanisms such as DNA de/methylation, histone modifications, as well as non-coding RNAs in promoting endothelial dysfunction in major arterial and venous diseases, consequent to hemodynamic alterations. We als...
Source: Molecular and Cellular Biochemistry - August 24, 2020 Category: Biochemistry Authors: Karthika CL, Ahalya S, Radhakrishnan N, Kartha CC, Sumi S Tags: Mol Cell Biochem Source Type: research